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Articles 3151 - 3180 of 9777

Full-Text Articles in Veterinary Medicine

Comparative Study Of Growth Patterns In Seven Strains Of Japanese Quail Using Nonlinear Regression Modeling, Hadi Farajiarough, Mohammad Rokouei, Ali Maghsoudi, Mahmoud Ghazaghi Jan 2018

Comparative Study Of Growth Patterns In Seven Strains Of Japanese Quail Using Nonlinear Regression Modeling, Hadi Farajiarough, Mohammad Rokouei, Ali Maghsoudi, Mahmoud Ghazaghi

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the growth pattern of seven strains of Japanese quail including Wild, Italian Speckled, Tuxedo, Scarlett, English white, White button, and A&M Texas by nonlinear growth functions including Gompertz, Richards, logistic, Lopez, and Weibull functions. The body weights of the unselected randomly bred populations of these strains were measured weekly from 1 to 42 days of age and then used to fit functions. The fitness of each function was assessed by statistics including the mean square error, Akaike information criterion, Bayesian information criterion, and adjusted coefficient of determination. The results showed that Gompertz …

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Severe Granulomatous Hepatitis Caused By Capillaria Hepatica In A Puppy, Ayli̇n Alasonyalilar Demi̇rer, Ahmet Akkoç, Bayram Şenli̇k, İbrahi̇m Taci̇ Cangül Jan 2018

Severe Granulomatous Hepatitis Caused By Capillaria Hepatica In A Puppy, Ayli̇n Alasonyalilar Demi̇rer, Ahmet Akkoç, Bayram Şenli̇k, İbrahi̇m Taci̇ Cangül

Turkish Journal of Veterinary & Animal Sciences

This report describes the inflammation caused by Capillaria hepatica in the liver of a female stray dog of approximately 2 months old for the first time in Turkey. Histopathological examination revealed severe granulomatous hepatitis with occasional adult nematodes and abundant eggs in the hepatic parenchyma. The present case differs from the other capillariasis cases by the age of the dog and the presence of mature female parasites and severe inflammation.

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Alterations Of Circulating Cortisol, Igf-1, And Thyroid Hormones In Darehshori Horses During A Year, Aliasghar Chalmeh, Mehrdad Pourjafar, Khalil Badiei, Mohammad Mazrouei Sebdani, Ali Sadeghpour, Hasan Sheikhi, Ali Khezri Jan 2018

Alterations Of Circulating Cortisol, Igf-1, And Thyroid Hormones In Darehshori Horses During A Year, Aliasghar Chalmeh, Mehrdad Pourjafar, Khalil Badiei, Mohammad Mazrouei Sebdani, Ali Sadeghpour, Hasan Sheikhi, Ali Khezri

Turkish Journal of Veterinary & Animal Sciences

Season may be considered as a factor that affects the equine endocrine profile. Hence, from January to November 2015, 27 clinically healthy adult Darehshori racehorses were selected. Thirteen of them were stallions and the others were mares. Ten of them were more than 10 years old and 17 horses were under the age of 10 years. None of them were pregnant or lactating during the study. Body condition scores of 12 horses were below and the others were above 5. Blood samplings and body condition score evaluations were performed every 45 days during a year. Serum levels of cortisol, IGF-1, …

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Determination Of Methicillin Resistance And Some Genotypic Characteristics Ofstaphylococci Isolated From Dogs And Their Owners, Arzu Findik, Alper Çi̇ftci̇, Taylan Önyay, Merve Gi̇zem Sezener, Yağmur Koçak, Ti̇mur Gülhan Jan 2018

Determination Of Methicillin Resistance And Some Genotypic Characteristics Ofstaphylococci Isolated From Dogs And Their Owners, Arzu Findik, Alper Çi̇ftci̇, Taylan Önyay, Merve Gi̇zem Sezener, Yağmur Koçak, Ti̇mur Gülhan

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the methicillin resistance and some genotypic characteristics of staphylococci in dogs and their owners. A total of 132 swab samples from 33 healthy dogs and their owners were taken. Isolated staphylococci were identified by PCR. The antibiotic sensitivities of the isolates were determined by disc diffusion method. Determination of pvl, mecA, bla, and fem genes and SCCmec types was performed by PCR. Isolates were genotyped according to coa and spa gene polymorphisms by PCR. Fortyfive isolates were identified as Stapyloccoccus spp. Among them, 8 isolates were identified as S. aureus and 23 isolates were identified …

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Molecular Detection Of Virulent Mannheimia Haemolytica And Pasteurella Multocida In Lung Tissues Of Pneumonic Sheep From Semiarid Tropics, Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Rajendra Kumar Meena Jan 2018

Molecular Detection Of Virulent Mannheimia Haemolytica And Pasteurella Multocida In Lung Tissues Of Pneumonic Sheep From Semiarid Tropics, Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Rajendra Kumar Meena

Turkish Journal of Veterinary & Animal Sciences

The present study was planned to detect the genetic elements of Mannheimia haemolytica and Pasteurella multocida in pneumonic sheep lungs. Pneumonia was diagnosed on the basis of gross pathological lesions. Lung tissues were collected at necropsy of sheep (n = 96) and subjected to isolation of total DNA. The M. haemolytica-specific PHSSA and Rpt2 genes, and the P. multocidaspecific KMT1 and the Omp87 genes were amplified using polymerase chain reaction (PCR). A housekeeping gene targeting the sheep cellular mitochondrial 12S ribosomal DNA was used as an internal control. PCR reactions were optimized using the positive and negative controls. Gene-specific PCR …

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Investigation Of The Efficiency And Safety Of Tilmicosin Phosphate In Treating Experimentalmycoplasmal Infections In Pigs, Xiaohui Zhang, Jinzhe Pan, Ning Wu, Shu Tang, Xiangdong Lei, Yangyang Sun, Joerg Hartung, Endong Bao Jan 2018

Investigation Of The Efficiency And Safety Of Tilmicosin Phosphate In Treating Experimentalmycoplasmal Infections In Pigs, Xiaohui Zhang, Jinzhe Pan, Ning Wu, Shu Tang, Xiangdong Lei, Yangyang Sun, Joerg Hartung, Endong Bao

Turkish Journal of Veterinary & Animal Sciences

In order to evaluate the in vivo therapeutic effect of oral administration of tilmicosin phosphate on mycoplasmal pneumonia in swine and its safety for the infected swine, the model of mycoplasmal pneumonia disease in swine was established by artificial infection, and indices such as efficiency, cure rate, death rate, the score of lung lesion, and the routine blood tests, blood biochemistry, and routine urine tests of tested swine were determined. The results showed that all of the dosage groups of 100 mg/L, 80 mg/L, 60 mg/L of 10% tilmicosin phosphate soluble powder demonstrated distinct therapeutic effects on mycoplasmal pneumonia in …

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A Stereological Study On Calculation Of Volume Values Of The Cervical Spinalsegments In Ducks, Gamze Çakmak, Hüseyi̇n Karadağ, Zafer Soygüder, Murat Çeti̇n Rağbetli̇, Serkan Yildirim, Haci Keleş Jan 2018

A Stereological Study On Calculation Of Volume Values Of The Cervical Spinalsegments In Ducks, Gamze Çakmak, Hüseyi̇n Karadağ, Zafer Soygüder, Murat Çeti̇n Rağbetli̇, Serkan Yildirim, Haci Keleş

Turkish Journal of Veterinary & Animal Sciences

In the present study, volume values of the cervical segments in the spinal cords of adult ducks weighing 3-4 kg were investigated. Volume values of the grey matter and white matter in the cervical segment of the spinal cord of the ducks were examined stereologically. Ten ducks (genus Anas) without regarding the sex of the animals. All animals were perfused using 10% formaldehyde. The animals were kept in 10% formaldehyde for 1 week to ensure fixation. The ducks were then dissected. The cervical segments were uncovered by removing the cervical vertebrae. Tissue samples were obtained from each of the cervical …

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Chronic Diarrhea Due To Lymphosarcoma In An Adult Cow: A Sporadic Clinical Report, Nuzhat Hassan, Charanjit Singh Randhawa, Swaran Singh Randhawa, Deepti Narang Jan 2018

Chronic Diarrhea Due To Lymphosarcoma In An Adult Cow: A Sporadic Clinical Report, Nuzhat Hassan, Charanjit Singh Randhawa, Swaran Singh Randhawa, Deepti Narang

Turkish Journal of Veterinary & Animal Sciences

The present clinical report describes a rare sporadic clinical case of chronic diarrhea due to lymphosarcoma in a 7-yearold crossbred Holstein?Friesian cattle. Diarrhea occurred due to intestinal lymphoid tissue involvement. The clinical diagnosis of lymphosarcoma was derived from peritoneal fluid aspirate, lymph node enlargement, and absence of serological and fecal evidence of any parasitic or bacterial infection. Reactive proliferative mesothelial hyperplasia in peritoneal fluid examination confirmed lymphosarcoma. The cells form numerous 3-dimensional clusters in mesothelial lymphosarcoma. Postmortem examinations confirmed visceral lymph node enlargements. A hematological examination revealed leukocytosis and a marked decrease in hemoglobin level, which reflected a severe degree …

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Systems Biology Approach For The Establishment Of Veterinary Bone Tissue Engineering: The Proteomics Of Cbm-Mscs And Cdpscs Osteogenic Models, Sirirat Nantavisai Jan 2018

Systems Biology Approach For The Establishment Of Veterinary Bone Tissue Engineering: The Proteomics Of Cbm-Mscs And Cdpscs Osteogenic Models, Sirirat Nantavisai

Chulalongkorn University Theses and Dissertations (Chula ETD)

The utilization of canine mesenchymal stem cells (cMSCs) with multipotent capabilities has been regarded for possible therapy of incorrigible bone disease. Although various sources of cMSCs show similar characteristics, they are different in osteogenic potential due to their original cellular sources. This study was designed to globally explore and analyze the in vitro differentiation potential and behavior of canine bone-marrow derived mesenchymal stem cells (cBM-MSCs) and canine dental pulp stem cells (cDPSCs) toward osteogenic lineage. An in vitro osteogenic differentiation potential of the cells was preliminarily compared in terms of alkaline phosphatase activity assay and Von Kossa staining. Global study …

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Seroepidemiology And Molecular Investigation Of Pestiviruses Among Sheep And Goats In Northwest Anatolia, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ Jan 2018

Seroepidemiology And Molecular Investigation Of Pestiviruses Among Sheep And Goats In Northwest Anatolia, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

Pestiviruses, which are distributed worldwide, may cause losses in sheep and goat populations. The current seroprevalence and circulation of pestiviruses in small ruminants of the South Marmara Region of Turkey were investigated. A total of 607 blood samples were collected from 22 different flocks in 4 different provinces, and 24 necropsy materials were investigated. Pestivirus seroprevalence in sheep and goats was determined by virus neutralization test against the NADL strain of bovine viral diarrhea virus. The mean antibody titer was 3.5, while the overall seropositivity rate was 32.78% (199/607). Seronegativity was markedly more prevalent in goats. The mean detected antibody …

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The Effects Of Dietary Biotechnological Products Of Saccharomices Cerevisiae On Growth Performance, Health Status, And Meat Composition In Broiler Chickens, Ionel Toader, Mihai Iacob Bentea, Ilie Cornoiu, Otilia Bobis Jan 2018

The Effects Of Dietary Biotechnological Products Of Saccharomices Cerevisiae On Growth Performance, Health Status, And Meat Composition In Broiler Chickens, Ionel Toader, Mihai Iacob Bentea, Ilie Cornoiu, Otilia Bobis

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to investigate the effects of three biotechnological products of Saccharomyces cerevisiae (organic selenium, concentrated mannan of S. cerevisiae, and living cells of the same yeast) used in broiler diet on growth performance, health status, and meat quality and composition. One hundred day-old broiler chickens were divided into four groups (control and three experimental groups using the above-mentioned diet supplements) and fed basic feed mixture (developed by the Nutrition Department at the University of Agricultural Sciences and Veterinary Medicine in Cluj-Napoca, Romania) for 42 days. After this period, from each group, five birds were sacrificed and …

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Development Of Septic Polysynovitis And Uveitis In Foals Experimentally Infected With Rhodococcus Equi, Laura Huber, Steeve Giguère, Londa J. Berghaus, Amanda Hanafi, Sarah Vitosh-Sillman, Sarah L. Czerwinski Jan 2018

Development Of Septic Polysynovitis And Uveitis In Foals Experimentally Infected With Rhodococcus Equi, Laura Huber, Steeve Giguère, Londa J. Berghaus, Amanda Hanafi, Sarah Vitosh-Sillman, Sarah L. Czerwinski

School of Veterinary and Biomedical Sciences: Faculty Publications

Rhodococcus equi is one of the most important causes of disease in foals. Infection is typically characterized by pyogranulomatous pneumonia although extrapulmonary infections occur occasionally. Uveitis and polysynovitis have been reported in foals naturally infected with R. equi and are thought to be the result of an immune-mediated process. However, the pathogenesis of these conditions is poorly understood. The objectives of this study were to document the occurrence of uveitis and polysynovitis after experimental infection with R. equi and to determine if these disorders are the direct result of infection at these sites. Foals between 3 and 4 weeks of …

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Circadian Behavioral Responses To Light And Optic Chiasm-Evoked Glutamatergic Epscs In The Suprachiasmatic Nucleus Of Iprgc Conditional Vglut2 Knock-Out Mice, Michael G. Moldavan, Patricia J. Sollars, Michael R. Lasarev, Charles N. Allen, Gary E. Pickard Jan 2018

Circadian Behavioral Responses To Light And Optic Chiasm-Evoked Glutamatergic Epscs In The Suprachiasmatic Nucleus Of Iprgc Conditional Vglut2 Knock-Out Mice, Michael G. Moldavan, Patricia J. Sollars, Michael R. Lasarev, Charles N. Allen, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

Intrinsically photosensitive retinal ganglion cells (ipRGCs) innervate the hypothalamic suprachiasmatic nucleus (SCN), a circadian oscillator that functions as a biological clock. ipRGCs use vesicular glutamate transporter 2 (vGlut2) to package glutamate into synaptic vesicles and light-evoked resetting of the SCN circadian clock is widely attributed to ipRGC glutamatergic neurotransmission. Pituitary adenylate cyclase-activating polypeptide (PACAP) is also packaged into vesicles in ipRGCs and PACAP may be coreleased with glutamate in the SCN. vGlut2 has been conditionally deleted in ipRGCs in mice [conditional knock-outs (cKOs)] and their aberrant photoentrainment and residual attenuated light responses have been ascribed to ipRGC PACAP release. However, …

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Application Of Needle-Free Injection Technology For Foot And Mouth Disease Vaccination Via Intradermal Route, Sirirat Wataradee Jan 2018

Application Of Needle-Free Injection Technology For Foot And Mouth Disease Vaccination Via Intradermal Route, Sirirat Wataradee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Foot and mouth disease (FMD) is an endemic disease in Thailand and caused severe economic loses. In order to prevent and control disease, the vaccination program is routinely administered for 2-3 times a year via subcutaneous route (SC). Even though, the vaccination is performed regularly, the outbreaks have often reported. The outbreaks occur due to the failure of number of immunity animal in population or herd immunity. The limitation of vaccine distribution to all animal population and difficulty of cattle restraint are forcible several farmers to failing to vaccinate their animals. Based on previous studies, the intradermal route (ID) can …

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Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum Jan 2018

Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum

Chulalongkorn University Theses and Dissertations (Chula ETD)

The main overall goal of this study is to investigate the application of modified chitosan as a potential vector for apoptotic gene delivery to gonadotropin-releasing hormone receptor (GnRHR)-expressing cells (i.e. testicular cells and mammary cancer cells). This study reported Gonadotropin Releasing Hormone-modified Chitosan (GnRH-CS) nanoparticle as a promising vector for targeted gene delivery, and a GnRH peptide was used for active targeting of a transgene in GnRHR expressing cells. The physiological characterizations of the prepared nanoparticle were investigated by proton nuclear magnetic resonance spectroscopy (1H NMR), Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and Nanosizer. The prepared GnRH-CS was …

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Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti Jan 2018

Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Diagnosis of Canine Monocytic Ehrlichiosis is in light of anamesis, visualized cytoplasmic morulae, clinical signs and laboratory test result of platelet counts. The PCR technique is always a definitive confirmatory test of Ehrlichia canis infection. However, the drawback of PCRs test is crucially needed of laboratory equipments. This drawback is somehow enabled newly DNA based technology to be developed in order to eliminate laborious thermocycler and gel electrophoresis. The LAMP-LFD is on focus by using a single temperature to amplify DNA and no gel electrophoresis involved to detect DNA product. LAMP-LFD is included in DNAsensor test kit that recently developed …

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The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya Jan 2018

The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya

Chulalongkorn University Theses and Dissertations (Chula ETD)

Monosodium glutamate (MSG) is composed of sodium salt and amino acids that is commonly used to enhance food taste. Although it is safe at consumption level, the adverse effect of MSG had been reported. The present research was intended to evaluate the effect of MSG on the brain, liver, kidney, testes, and ovaries of zebrafish (Danio rerio) which were exposed from embryo stage to adult stage, along with the analyzing of the fertilization rate and hatching rate of zebrafish embryos. From the observation of histopathological changing of different organs of zebrafish after exposed to 10, 100, and 1000 ppm MSG …

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Synbiotic Effects Of Jerusalem Artichoke (Helianthus Tuberosus) And Lactobacillus Rhamnosus-Supplemented Diet On Growth Performance, Immunological Parameters And Protection Against Aeromonas Veronii Challenge In Juvenile Red Tilapias (Oreochromis Spp.), Mariya Sewaka Jan 2018

Synbiotic Effects Of Jerusalem Artichoke (Helianthus Tuberosus) And Lactobacillus Rhamnosus-Supplemented Diet On Growth Performance, Immunological Parameters And Protection Against Aeromonas Veronii Challenge In Juvenile Red Tilapias (Oreochromis Spp.), Mariya Sewaka

Chulalongkorn University Theses and Dissertations (Chula ETD)

Synbiotics, a synergistic combination of probiotics and prebiotics, are now highlighted as one of the most practical nutritional supplements in red tilapia farms. The aim of the present study was to assess the effect of the dietary inclusion of Jerusalem artichoke, Lactobacillus rhamnosus, or their combination, on serum biochemical, growth performance, intestinal morphology, immune parameters, intestinal bacterial count and protection against Aeromonas veronii in juvenile red tilapia (Oreochromis spp.). One hundred and eighty male red tilapias (average body weight of 14.05±0.42 g) were distributed into six 1000-liter tanks containing water under continuous aeration and an average temperature of 25–28°C. Tilapias …

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Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij Jan 2018

Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brugia pahangi is a major causative agent of animal lymphatic filariasis, one of the neclected tropical disease. Recent studies have also indicated that B. pahangi can cause clinical infection in human, with clinical appearances which are consistent with human lymphatic filariasis. Nowadays, the recognition of Wolbachia as new alternative drug choice for treatment and control of lymphatic filariasis is the most important in anti-filarial chemotherapy. Since, Wolbachia play important roles, including the development, viability, fertility and fecundity of filarial parasites. Depletion of Wolbachia results in sterilization, stunting and death of filarial worms. In this study, we investigated the effects of …

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Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen Jan 2018

Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Retinal (retinaldehyde) is one of natural vitamin A metabolites. It is widely used in clinical dermatology such as acne, acne scar, photoaging, wrinkles, psoriasis, skin neoplasia and seborrheic dermatitis. Retinal is a pivotal key to regulate keratinocyte proliferation and differentiation in the epidermis resulting in epidermal thickening and inhibit collagen destruction in the dermis. However, topical application of retinal is still irritative to the skin and chemically and photochemically unstable. Topically applied retinal can induce a retinoid dermatitis. This inflammation is induced by an overload of non-physiological amounts of exogenous retinoic acid in the skin. The adverse effects and physicochemical …

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Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn Jan 2018

Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Flavobacterium columnare is Gram negative bacteria which caused columnaris disease in various kinds of freshwater fishes. This study is the first report of F. columnare infection in freshwater culturing Asian sea bass in Thailand. Phenotypic characterization, antimicrobial susceptibility test and quinolone resistant-associated genes of F. columnare were performed. A total 15 of F. columnare were isolated from diseased fishes and phylogenetic analysis revealed that these isolates were belonged to genetic group 2 and 4. From disk diffusion test results, 5 and 6 isolates were resistant to oxolinic (OA) and nalidixic acid (NA) respectively. The highest MIC values to OA and …

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Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain Jan 2018

Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain

Chulalongkorn University Theses and Dissertations (Chula ETD)

Live bird markets play a significant role in highly pathogenic avian influenza (HPAI) virus spread and transmission. In Myanmar, the first HPAI-H5N1 outbreak was reported in 2006. Since 2014, routine influenza A virus surveillance in LBMs have been conducted in Myanmar by the Livestock Breeding and Veterinary Department in cooperation with FAO. However, there is limited information on virus characterization and evolution of influenza viruses in Myanmar. The objectives of this thesis were to detect influenza virus subtypes circulating in different poultry species from Mingalar-Taung-Nyunt live bird market (MTN-LBM) and to determine genetic characteristics and genetic diversity of influenza A …

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Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri Jan 2018

Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Campylobacter has been known as an important cause of gastroenteritis in humans. The primary source is raw or undercooked poultry meat. Previous study showed that Campylobacter isolated from slaughterhouses were associated with Campylobacter isolated from broiler farms. Thus, detection of Campylobacter flock status and sorting flocks prior to slaughtering processes may help prevent Campylobacter contamination between Campylobacter-positive and negative flocks during slaughtering processes. Unfortunately, appropriate sample type and isolation method have not been clearly specified for Campylobacter detection at the farm level. Because Campylobacter are fastidious bacteria, improper detection method can attribute to false negative results. Therefore, the objectives of …

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Development Of Reverse Transcription Loop-Mediated Isothermal Amplification Combine With Lateral Flow Device As A Detection Assay For Swine Influenza Virus, Albarriyati Ummi Asih Jan 2018

Development Of Reverse Transcription Loop-Mediated Isothermal Amplification Combine With Lateral Flow Device As A Detection Assay For Swine Influenza Virus, Albarriyati Ummi Asih

Chulalongkorn University Theses and Dissertations (Chula ETD)

Swine influenza causes high morbidity and low mortality in pigs. The disease spreads rapidly in pig farms causing the delay of pig weight to reach market. Moreover, pigs play important role as "mixing vessel" of influenza viruses. Pigs can support infection and replication of influenza viruses from avian, human and swine origins. These lead to major concerns of the important of swine influenza which are not only the economic impacts but also the human health. There is the need of accurate and rapid diagnosis for swine influenza virus (SIV) to minimize further spreading of the viruses in pig population and …

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Pedv Monitoring Using Oral Fluid, Feces And Surface Swab Samples After The Ped Outbreak, Busayamas Jantrasakul Jan 2018

Pedv Monitoring Using Oral Fluid, Feces And Surface Swab Samples After The Ped Outbreak, Busayamas Jantrasakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 220 pooled samples from 3 collection times were conducted. Eighty pooled samples from the first collection at 1 month after PED outbreak (1MAO) included 10 pooled oral fluid samples, 30 pooled fecal samples and 40 pooled surface swab samples. Eighty-five pooled samples from the second collection at 2MAO included 10 pooled oral fluid samples, 30 pooled fecal samples and 45 pooled surface swab samples. Fifty-five pooled samples from the third collection at 8MAO included 5 pooled oral fluid samples, 15 pooled fecal samples and 35 pooled surface swab samples. All samples collected from the PED-affected swine farm …

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Phenotypic And Genotypic Characteristics Of Extended-Spectrum Β-Lactamase (Esbl) Production And Colistin-Resistance In Salmonella Enterica And Escherichia Coli Isolated From​ Pigs And Their Meat Products In The Border Provinces Between Thailand And Cambodia, Lao Pdr And Myanmar, Kyaw Phyoe Sunn Jan 2018

Phenotypic And Genotypic Characteristics Of Extended-Spectrum Β-Lactamase (Esbl) Production And Colistin-Resistance In Salmonella Enterica And Escherichia Coli Isolated From​ Pigs And Their Meat Products In The Border Provinces Between Thailand And Cambodia, Lao Pdr And Myanmar, Kyaw Phyoe Sunn

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 809 samples of pig rectal swab from slaughterhouses (n=441) and pork from retail market (n=368) were collected in the border provinces among Thailand, Cambodia, Lao PDR and Myanmar between October 2016 and March 2017. The objective of this study was to determine resistance to extended-spectrum ß-lactams (ESBLs) and colistin in Salmonella enterica and Escherichia coli. A total of Salmonella (n=463) and E. coli (n=767) were collected and determined for the ESBL-production and for the Minimum Inhibitory Concentrations (MICs) of colistin and; the presence of ESBL gene and mcr gene. The results showed that the prevalence of Salmonella …

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Genetic Characteristics Of Avian Influenza Viruses Isolated From Ducks In Java Island, Indonesia Year 2015-2017, Lestari - Jan 2018

Genetic Characteristics Of Avian Influenza Viruses Isolated From Ducks In Java Island, Indonesia Year 2015-2017, Lestari -

Chulalongkorn University Theses and Dissertations (Chula ETD)

Avian influenza becomes public health concern over years due to the serious impacts in both animal and human health. This study aimed to identify and characterize avian influenza viruses isolated from ducks in Java Indonesia during 2015-2017. Total 50 samples of previously identified as avian influenza viruses were acquired from the virus/culture collections of Disease Investigation Center (DIC) Wates Yogyakarta, Indonesia. The samples were recovered from either the avian influenza surveillance or avian influenza outbreaks in ducks in 3 provinces, East Java (n=26), Central Java (n=16), and Yogyakarta (n=8). Then the samples were processed for influenza A virus screening by …

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Genome Characterization Of Hahella Chejuensis Strain Hn01, A Pathogen Of Red Egg Disease In Tilapia (Oreochromis Sp.), Putu Cri Devischa Gallantiswara Jan 2018

Genome Characterization Of Hahella Chejuensis Strain Hn01, A Pathogen Of Red Egg Disease In Tilapia (Oreochromis Sp.), Putu Cri Devischa Gallantiswara

Chulalongkorn University Theses and Dissertations (Chula ETD)

Red eggs disease is an emerging disease occurred in Tilapia hatcheries which caused by a Gram-negative bacterium called Hahella chejuensis HN01. The disease infects to all the stage of fish eggs and reduces the hatchability. However, there are limited study about the bacterial pathogenicity. In this study, we analyzed the phylogenetic relationship and characterized the virulence traits of Hahella chejuensis by using genomic approach. The bacterial genome was sequenced using Illumina Miseq platform. The phylogenetic tree based on several housekeeping genes confirmed that the bacterium was belong to Genus Hahella and species chejuensis. the Average Nucleotide Identity calculation with the …

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Detection Of Antimicrobial Resistance Determinants In Vibrio Parahaemolyticus Isolated From Cultivated Oysters And Estuarine Waters, Winn Khant Jan 2018

Detection Of Antimicrobial Resistance Determinants In Vibrio Parahaemolyticus Isolated From Cultivated Oysters And Estuarine Waters, Winn Khant

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, we aimed to detect antimicrobial resistance determinants and virulence factor genes in V. parahaemolyticus isolates from cultivated oysters and estuarine waters in southern Thailand. A total of 594 V. parahaemolyticus isolates were collected from pooled oysters (n=361) and estuarine waters (n=233). The samples were collected monthly between March 2016 and February 2017 from Thap Put district, Phang Nga province in southern Thailand. Confirmation of V. parahaemolyticus was carried out by PCR assay detecting species-specific (tlh) gene and the presence of virulence genes (tdh and trh). Antimicrobial susceptibility test was performed in eight antimicrobials, and the occurrence of …

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Biomechanical And Biological Properties Of Cryopreserved Canine Amniotic Membrane, Nathawan Withavatpongtorn Jan 2018

Biomechanical And Biological Properties Of Cryopreserved Canine Amniotic Membrane, Nathawan Withavatpongtorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Amniotic membrane (AM) is the innermost fetal membrane. Due to its many known properties, human AM is a favorable corneal graft. Cryopreservation at -80ºC maintains most of biological properties. Due to shortage of supply, AM of other species are studied. The objective of this study is to explore the biomechanical and biological properties of canine AM (CAM) after cryopreservation for 7 and 30 days. CAMs were collected from healthy bitches undergoing cesarean sections. After preservation, they were tested for tensile strength by Universal Testing Machine, transparency by spectrophotometer, sterility by microbial culture, cell viability by trypan blue staining, and histological …

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