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Articles 2941 - 2970 of 9758

Full-Text Articles in Veterinary Medicine

Oxidative Stress And Antioxidant In Canine Cutaneous Mast Cell Tumors, Arayaporn Macotpet, Ekkachai Pattarapanwichien, Fanan Suksawat, Patcharee Boonsiri Dec 2018

Oxidative Stress And Antioxidant In Canine Cutaneous Mast Cell Tumors, Arayaporn Macotpet, Ekkachai Pattarapanwichien, Fanan Suksawat, Patcharee Boonsiri

The Thai Journal of Veterinary Medicine

Oxidative stress can result from either the excessive production of reactive oxygen species (ROS) or an impaired antioxidant system, or both. It causes damage to lipids, proteins and DNA. Therefore, oxidative stress may be involved in carcinogenesis, and is associated with many types of cancer in dogs. The objective of this study was to compare the levels of malondialdehyde, protein hydroperoxides, glutathione, retinol and alpha-tocopherol between dogs with mast cell tumors and clinically healthy controls. Blood samples were obtained from eighteen clinically healthy dogs and fourteen dogs with spontaneous mast cell tumors. Malondialdehyde and protein hydroperoxides levels were measured by …

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Impact Of Feed Characteristics For Monitoring Afb1 Contamination In Commercial Concentrate Feeds Used In Small Holder Dairy Farms, Chiangmai, Thailand, Wasana Chaisri, Wantanwa Mongkon, Yoshigo Sugita-Konishi, Witaya Suriyasathaporn Dec 2018

Impact Of Feed Characteristics For Monitoring Afb1 Contamination In Commercial Concentrate Feeds Used In Small Holder Dairy Farms, Chiangmai, Thailand, Wasana Chaisri, Wantanwa Mongkon, Yoshigo Sugita-Konishi, Witaya Suriyasathaporn

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the impact of abnormal physical characteristics for monitoring aflatoxin B1 (AFB1) contamination in commercial dairy concentrate feeds. The study was conducted during March to May 2013 using 82 dairy farms in Chiang Mai province, Thailand. All feed samples were collected from new packages and their physical characteristics were subsequently determined. AFB1 concentrations in the samples were measured using the enzyme-linked immunosorbent assay (ELISA), and feed samples with AFB1 of more than 5 µg/Kg were determined as AFB1 contaminated samples. The impact of physical characteristics on AFB1 contamination were evaluated using a multiple …

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Comparative Effect Of Dl-Methionine And Dl-Methionine Hydroxy Analogue Supplemented Diet On Productive Performance, Fat Accumulation And Lipid Profile In Blood Of Meat-Type Ducks, Chanwit Kaewtapee, Chaiyapoom Bunchasak Dec 2018

Comparative Effect Of Dl-Methionine And Dl-Methionine Hydroxy Analogue Supplemented Diet On Productive Performance, Fat Accumulation And Lipid Profile In Blood Of Meat-Type Ducks, Chanwit Kaewtapee, Chaiyapoom Bunchasak

The Thai Journal of Veterinary Medicine

The objective of this study was to compare the effect of DL-methionine (DLM) and DL-methionine hydroxy analogue (DL-MHA) supplementation on the growth performance, carcass quality, fat accumulation and lipid profile in blood of meat-type duck. Three hundred male Cherry Valley ducks were divided into 3 groups with 4 replications of 25 ducklings each. Each group was given a diet as follows: 1) basal diet, 2) basal diet + DLM, and 3) basal diet + DLMHA. The results show that dietary supplementation with DLM and DL-MHA increased body weight (P<0.01), feed intake (P<0.01) and pectoralis major (P<0.05), but decreased abdominal fat (P<0.01) when compared to the basal diet. Supplementation with DL-MHA also resulted in greater levels of triglycerides (P<0.05), low-density lipoprotein (P<0.05) and cholesterol (P<0.05) in the blood compared to the DLM group, in addition to greater levels of triglycerides (P<0.05) and high-density lipoprotein (P<0.05) compared to the basal diet. Furthermore, supplementation with DLM was shown to lower the level of plasma uric acid (P<0.05) compared to the basal diet. It is concluded that DL-MHA can promote the same productive performance in meat-type ducks as DLM, but the fat accumulation and lipid profile in blood was greater with the DL-MHA supplementation than with DLM. Furthermore, the underlying mechanism of different effects between DL-MHA and DLM on the lipid metabolism in ducks should be further investigated.

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Emergence Of A Classical Variant Of Porcine Epidemic Diarrhea Virus Novel To Thailand Responsible For The Milder Clinical Disease In A Herd Previously Infected With A Pandemic Variant, Gun Temeeyasen, Anchalee Srijangwad, Thitima Tripipat, Angkana Tantituvanont, Dachrit Nilubol Dec 2018

Emergence Of A Classical Variant Of Porcine Epidemic Diarrhea Virus Novel To Thailand Responsible For The Milder Clinical Disease In A Herd Previously Infected With A Pandemic Variant, Gun Temeeyasen, Anchalee Srijangwad, Thitima Tripipat, Angkana Tantituvanont, Dachrit Nilubol

The Thai Journal of Veterinary Medicine

In 2014, porcine epidemic diarrhea (PED) outbreaks characterized by mild clinical disease and low preweaning mortality increased in frequency in Thailand. An outbreak investigation, based on the detection of the classical variant and the allowance of the herd owners, was conducted in 5 swine herds and 25 intestinal samples were submitted from herds with outbreaks of diarrhea for sequencing analysis. PEDV isolation and complete nucleotide sequences of the spike (S) glycoprotein were determined. The PEDV isolate EAS1 was identified. Phylogenetic analysis based on the full-length spike gene demonstrated that EAS1 belonged to the classical variant group with high nucleotide and …

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Comparative Ultrasonographic And Computed Tomographic Images Of The Adrenal Glands Of Healthy Cats, Panrawee Phoomvuthisarn, Kiatpichet Komin, Nan Choisunirachon Dec 2018

Comparative Ultrasonographic And Computed Tomographic Images Of The Adrenal Glands Of Healthy Cats, Panrawee Phoomvuthisarn, Kiatpichet Komin, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

Feline adrenal disorders that affect the size of the adrenal glands can be detected by diagnostic imaging including ultrasonography and Computed Tomography (CT). The objectives of this study were to compare the appearance of the adrenal gland in healthy cats between these two techniques and establish the reference values of the CT dimensions of the adrenal gland in cats. We found that there was no significant difference in the length of both adrenal glands between the two techniques but the cranial and caudal heights of the left adrenal glands and cranial height of the right adrenal gland obtained from the …

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Ophthalmology Snapshot, Nalinee Tuntivanich Dec 2018

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

A 6-month-old French Bulldog had been presented with left eye blepharospasm. The puppy had been treated with topical antibiotics for weeks from the local veterinarian. However, blepharospasm still persisted. Ophthalmic examination from distance showed mild conjunctival hyperemia. Excessive tear was observed around the eyeball. Close ophthalmic examinations revealed prominent conjunctival blood vessels at the outer portion of the nictitating membrane. Clump of abnormal hairs were identified at the ventro-nasal region of the palpebral conjunctiva, next to eyelid margin. Schirmer tear test 1 level of the left eye was 25 mm wetness, while it was 16 on the right.

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Microbiota Metabolic Product Deoxycholic Acid Prevents Campylobacter Jejuni Chicken Colonization Through Modulating Ceca Anaerobes, Bilal Ali Alrubaye Dec 2018

Microbiota Metabolic Product Deoxycholic Acid Prevents Campylobacter Jejuni Chicken Colonization Through Modulating Ceca Anaerobes, Bilal Ali Alrubaye

Graduate Theses and Dissertations

Campylobacter jejuni is a prevalent infectious enteritis mainly foodborne from chickens. Despite of reducing C. jejuni food contamination dramatically decreases campylobacteriosis, few effective approaches are available for the bacterial reduction in chickens. The aim of this study was to use microbial metabolic product deoxycholic acid (DCA) to reduce C. jejuni chicken colonization. Broiler chicks were fed 0 or 1.5 g/kg DCA, lithocholic acid (LCA), or urodeoxycholic acid (UDCA) in diets or orally gavaged with cholic acid (CA, 1.5g/kg body weight). Birds were also transplanted with DCA modulated anaerobes (DCA-Anearo) or aerobes (DCA-Aero). Birds were infected with 109 CFU/bird human clinical …

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The Advancements Of Feline Renal Allograft Transplantation Techniques And Treatments, Morgan Simon Nov 2018

The Advancements Of Feline Renal Allograft Transplantation Techniques And Treatments, Morgan Simon

Senior Honors Theses

In recent years, kidney transplants have become a viable treatment option for chronic kidney disease in felines. Chronic kidney disease is the leading disease in geriatric cats with 20% of these geriatric cats being affected. Feline kidney transplantation has been met with many different issues regarding ethics, diagnosis, methods, and complications. There are some individuals that believe kidney transplants should not be done in felines because consent from the feline cannot be obtained. The diagnosis of chronic kidney disease has been on the rise in recent years which may be due to new classification standards. There have been many different …

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Assessment Of Recent Cases Of Animal Hoarding In Germany: The Challenge For Animal Shelters And Public Authorities, Sophie Arnold, Henriette Mackensen, Evelyn Ofensberger, Brigitte Rusche Nov 2018

Assessment Of Recent Cases Of Animal Hoarding In Germany: The Challenge For Animal Shelters And Public Authorities, Sophie Arnold, Henriette Mackensen, Evelyn Ofensberger, Brigitte Rusche

People and Animals: The International Journal of Research and Practice

Animal hoarding is a severe problem in the field of human-animal interaction. The goal of this study was to assess the current situation of animal hoarding in Germany. Reports of animal hoarding cases were collected from animal shelters and public media between January 2012 and December 2015; 120 cases were analyzed. A total of 9,174 animals were hoarded during the investigated time period. The results showed that cases involving cats were most common, followed by cases involving dogs and small mammals. The average number (x¯) of animals hoarded per case was 76 (x˜ = 43). Small mammals were hoarded in …

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Improved Reference Genome For The Domestic Horse Increases Assembly Contiguity And Composition, Theodore S. Kalbfleisch, Edward S. Rice, Michael S. Depriest Jr., Brian P. Walenz, Matthew S. Hestand, Joris R. Vermeesch, Brendan L. O'Connell, Ian T. Fiddes, Alisa O. Vershinina, Nedda F. Saremi, Jessica L. Petersen, Carrie J. Finno, Rebecca R. Bellone, Molly E Mccue, Samantha A. Brooks, Ernest Bailey, Ludovic Orlando, Richard E. Green, Donald C. Miller, Douglas F. Antczak, James N. Macleod Nov 2018

Improved Reference Genome For The Domestic Horse Increases Assembly Contiguity And Composition, Theodore S. Kalbfleisch, Edward S. Rice, Michael S. Depriest Jr., Brian P. Walenz, Matthew S. Hestand, Joris R. Vermeesch, Brendan L. O'Connell, Ian T. Fiddes, Alisa O. Vershinina, Nedda F. Saremi, Jessica L. Petersen, Carrie J. Finno, Rebecca R. Bellone, Molly E Mccue, Samantha A. Brooks, Ernest Bailey, Ludovic Orlando, Richard E. Green, Donald C. Miller, Douglas F. Antczak, James N. Macleod

Maxwell H. Gluck Equine Research Center Faculty Publications

Recent advances in genomic sequencing technology and computational assembly methods have allowed scientists to improve reference genome assemblies in terms of contiguity and composition. EquCab2, a reference genome for the domestic horse, was released in 2007. Although of equal or better quality compared to other first-generation Sanger assemblies, it had many of the shortcomings common to them. In 2014, the equine genomics research community began a project to improve the reference sequence for the horse, building upon the solid foundation of EquCab2 and incorporating new short-read data, long-read data, and proximity ligation data. Here, we present EquCab3. The count of …

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Checklist Of Bloodfeeding Mites (Acari: Spinturnicidae) From The Wings Of Bats (Mammalia: Chiroptera) In The Manú Biosphere Reserve, Peru, Donald Gettinger Oct 2018

Checklist Of Bloodfeeding Mites (Acari: Spinturnicidae) From The Wings Of Bats (Mammalia: Chiroptera) In The Manú Biosphere Reserve, Peru, Donald Gettinger

MANTER: Journal of Parasite Biodiversity

A survey collection of mites of the family Spinturnicidae from Peruvian bats includes 11 species of Periglischrus (acutisternus, gameroi, grandisoma, herrerai, hopkinsi, iheringi, micronycteridis, ojasti, paracutisternus, paravargasi, and ramirezi) and 2 Spinturnix (americanus and bakeri); almost all represent new locality records. This survey collection is available for further study at the following repositories: The Harold W. Manter Laboratory of Parasitology, University of Nebraska–Lincoln; the Field Museum of Natural History, Chicago; and the Laboratório de Espeleobiologia y Acarologia, Universidad Nacional Autónoma de México. When spinturnicid mites are …

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Lasix Lessons Learned: Science Confirms What American Horsemen Knew 50 Years Ago, Clara Fenger, Steve Selway, Thomas Tobin Sep 2018

Lasix Lessons Learned: Science Confirms What American Horsemen Knew 50 Years Ago, Clara Fenger, Steve Selway, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.

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Investigation Into Toxin And Slime Genes In Staphylococci Isolated From Goat Milk And Goat Cheese In Southern Turkey, Sevda Pehlivanlar Onen, Zafer Cantekin, Faiza Mebkhout, Osman Aygun, Yasar Ergun Sep 2018

Investigation Into Toxin And Slime Genes In Staphylococci Isolated From Goat Milk And Goat Cheese In Southern Turkey, Sevda Pehlivanlar Onen, Zafer Cantekin, Faiza Mebkhout, Osman Aygun, Yasar Ergun

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the presence of Staphylococcus aureus and coagulase-negative staphylococci (CNS) isolated from goat milk and cheese, as well as their toxin genes and slime genes. S. aureus and CNS isolates were subjected to polymerase chain reaction (PCR) analyses to determine the prevalence of enterotoxin (sea, seb, sec, sed, see), toxic shock syndrome (TSS) toxin (tst), exfoliative toxin (eta and etb) and slime genes (icaA and icaD). sec, sed and tst genes together were detected in 4 (33.3%), sec + tst genes in 2 (16.7%) and tst gene in 1 (8.3%) of the 12 …

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Current Situation Of Neospora Caninum And Toxoplasma Gondii Infection Among Beef Cattle In Kanchanaburi, Ratchaburi And Nakhon Patom Provinces, Thailand, Ruenruetai Udonsom, Yaowalark Sukthana, Yoshifumi Nishikawa, Ragab M. Fereig, Charoonluk Jirapattharasate Sep 2018

Current Situation Of Neospora Caninum And Toxoplasma Gondii Infection Among Beef Cattle In Kanchanaburi, Ratchaburi And Nakhon Patom Provinces, Thailand, Ruenruetai Udonsom, Yaowalark Sukthana, Yoshifumi Nishikawa, Ragab M. Fereig, Charoonluk Jirapattharasate

The Thai Journal of Veterinary Medicine

Toxoplasmosis and neosporosis are parasitic diseases of livestock globally. In this study, we investigate the presence of Neospora caninum and Toxoplasma gondii in 250 beef cattle from Kanchanaburi, Ratchaburi and Nakhon Patom provinces, Thailand comparing with the results from 5 years earlier. The serological diagnosis for toxoplasmosis and neosporosis were performed by indirect ELISA technique using recombinant antigens TgGRA7 and NcSAG1, respectively. Furthermore, risk factors at the animal- and herd-levels were also evaluated. Seroprevalence of T. gondii, N. caninum and co-infections were 15.2%, 20.8% and 27.2%, respectively. In comparison to the results from 5 years earlier, seropositivity of N. caninum …

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Novel Methods To Diagnose Pulmonary Hypertension In Dogs Assessed By Thoracic Radiography, Kawinthip Chanroon, Thanat Tuntipas, Promsin Sukpanich, Pawit Punyarit, Sirilak Disatian Surachetpong Sep 2018

Novel Methods To Diagnose Pulmonary Hypertension In Dogs Assessed By Thoracic Radiography, Kawinthip Chanroon, Thanat Tuntipas, Promsin Sukpanich, Pawit Punyarit, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

The objective of this retrospective study was to distinguish between dogs with pulmonary hypertension (PH) and non-PH by using parameters obtained from thoracic radiographs. Data of 72 dogs of small breeds aged more than 1 year old and weighing less than 10 kg were retrieved. Echocardiography was used as a gold standard to diagnose PH and separate the dogs into two groups: PH (n=15) and non-PH (n=57). Seven formulae were created from measurements on right lateral and ventrodorsal radiographic views. Five formulae from the ventrodorsal view provided an outcome that could differentiate PH and non-PH dogs. Two formulae are recommended …

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The Effects Of Submaximal Exercise Training On Cardiovascular Functions And Physical Capacity In Dogs With Myxomatous Mitral Valve Disease, Saikaew Sutayatram, Chollada Buranakarl, Anusak Kijtawornrat, Kumpanart Soontornvipart, Pakit Boonpala, Prapawadee Pirintr Sep 2018

The Effects Of Submaximal Exercise Training On Cardiovascular Functions And Physical Capacity In Dogs With Myxomatous Mitral Valve Disease, Saikaew Sutayatram, Chollada Buranakarl, Anusak Kijtawornrat, Kumpanart Soontornvipart, Pakit Boonpala, Prapawadee Pirintr

The Thai Journal of Veterinary Medicine

Myxomatous mitral valve disease (MMVD), an important disease in senile small breed dogs, leads to deterioration of cardiovascular function and impairment of functional capacity resulting in poor quality of life. Submaximal treadmill running has been used as both cardiovascular training and testing worldwide in humans and recently in animals. The objectives of this study were to examine the effects of submaximal endurance training (ET) by treadmill running on safety, adverse events, cardiovascular adaptations, hematology, functional capacity, and the feasibility of submaximal treadmill running test and 6-minute walk test (6-MWT) in dogs with MMVD. Six adult beagle dogs with MMVD, stage …

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Evaluation Of A Commercial Elisa Test Kit On Classical Swine Fever Antibody Detection Using Oral Fluid Samples, Panchan Sitthicharoenchai, Yonlayong Woonwong, Korakrit Poonsuk, Jirapat Arunorat, Chonnatcha Muangpaisarn, Kanokwan Samatiwat, Worapatch Konthong, Whannaphorn Sattathara, Roongroje Thanawongnuwech Sep 2018

Evaluation Of A Commercial Elisa Test Kit On Classical Swine Fever Antibody Detection Using Oral Fluid Samples, Panchan Sitthicharoenchai, Yonlayong Woonwong, Korakrit Poonsuk, Jirapat Arunorat, Chonnatcha Muangpaisarn, Kanokwan Samatiwat, Worapatch Konthong, Whannaphorn Sattathara, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Classical swine fever virus (CSFV) contributes to economic loss of swine production in endemic countries. Serum neutralization and enzyme-linked immunosorbent assay (ELISA) are serological tests commonly used for monitoring CSFV antibody status with serum samples. This experiment evaluated the detection of CSFV antibody in oral fluid samples in commercial ELISA test kit by in vitro study of negative oral fluid mixed with serum of known CSFV serum neutralizing (SN) titer and in vivo oral fluid samples obtained from experimental animals. Correlations of SN titer and S/P ratio of ELISA were observed with the in vitro oral fluid samples, indicating the …

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Effects Of Coenzyme Q10 Supplementation On Cardiac Troponin I Level, Heart Rate Variability, And Echocardiographic Profiles In Canine With Myxomatous Degenerative Mitral Valve Disease: A Pilot Study, Kittipong Tachampa, Tuchakorn Lertwanakarn, Panupong Atchariyasakchai, Vichayanee Pumpitakkul, Supapatt Kireewan, Chollada Buranakarl Sep 2018

Effects Of Coenzyme Q10 Supplementation On Cardiac Troponin I Level, Heart Rate Variability, And Echocardiographic Profiles In Canine With Myxomatous Degenerative Mitral Valve Disease: A Pilot Study, Kittipong Tachampa, Tuchakorn Lertwanakarn, Panupong Atchariyasakchai, Vichayanee Pumpitakkul, Supapatt Kireewan, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

The clinical benefits of Coenzyme Q10 (CoQ10) have been well documented in heart failure patients. In veterinary medicine, however, the clinical benefits of CoQ10 have not been well established in dogs with heart diseases. This study aimed to determine the effects of CoQ10 supplementation on 1) preventing myocardial injury 2) cardiac function 3) autonomic balance in dogs with myxomatous degenerative mitral valve disease (MMVD). Thirteen dogs with MMVD at ACVIM stage C were recruited in this study. Dogs were further divided into group I (Body weight [BW] < 6 kg.; n = 7) and group II (BW ≥ 6 kg.; n = 6). Cardiac troponin I (cTnI), echocardiographic examination, and 2 hours of Holter recording were performed on Day 0 and after receiving CoQ10 at 100 mg/dog twice daily for 28 days. CoQ10 caused a reduction of cTnI level in 71% of the dogs. Median cTnI tended to cause tended to decline but not significantly. Systolic function [i.e. fractional shortening (FS) and ejection fraction (EF)] increased significantly by 8 % and 9% (P < 0.05), respectively in group I after CoQ10 supplementation. Other echocardiographic parameters were not altered in either group. HRV analysis revealed no change in autonomic function and balance. cTnI negatively correlated with FS (P < 0.01) and positively correlated with LF/HF ratio (P < 0.05). In conclusion, this study demonstrated the clinical benefits of CoQ10 supplementation in dogs with MMVD. CoQ10 improves cardiac function in small dogs with MMVD. Finally, supplementation of CoQ10 in dogs should be based on body weight.

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Syrian Hamster (Mesocricetus Auratus) As An Animal Model For Haplorchis Taichui Infection: Biology, Morphology, And Pathology, Jiraporn Songsri, Thidarut Boonmars, Sirintip Boonjaraspinyo, Ratchadawan Aukkanimart, Pranee Sriraj, Parichart Boueroy, Nadchanan Wongchalee Sep 2018

Syrian Hamster (Mesocricetus Auratus) As An Animal Model For Haplorchis Taichui Infection: Biology, Morphology, And Pathology, Jiraporn Songsri, Thidarut Boonmars, Sirintip Boonjaraspinyo, Ratchadawan Aukkanimart, Pranee Sriraj, Parichart Boueroy, Nadchanan Wongchalee

The Thai Journal of Veterinary Medicine

Haplorchis taichui is a trematode that can mature in the small intestine of mammals and birds, including humans. Larval stages occur in various invertebrate and cold-blooded vertebrate hosts. The purpose of this study was to investigate parasite growth, including worm recovery rate, the body size of adult worms, eggs per worm and eggs per gram of feces and pathological changes of the small intestine in experimental hamsters. In this study, 6- to 8-weekold male Syrian hamsters (Mesocricetus auratus) were each infected with 200 H.taichui metacercariae and then sacrificed at weeks 1, 2, 3, 4, 6 and 8 post-infection. The small …

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Development Of An Insoluble Hyaluronic Acid Membrane As An Effective Material For The Prevention Of Post-Thoracotomy Pleural Adhesions In Dogs, Akiko Uemura, Takashi Tanaka, Pinkarn Chantawong, Pitipat Kitpipatkun, Ryou Tanaka Sep 2018

Development Of An Insoluble Hyaluronic Acid Membrane As An Effective Material For The Prevention Of Post-Thoracotomy Pleural Adhesions In Dogs, Akiko Uemura, Takashi Tanaka, Pinkarn Chantawong, Pitipat Kitpipatkun, Ryou Tanaka

The Thai Journal of Veterinary Medicine

A novel membrane was previously found to be effective in preventing post-thoracotomy pleural adhesions in dogs. The aim of the present study was to verify the animals’ clinical condition, the biological fate of the residual membrane, the presence or absence of pleural adhesions, and any inflammatory reactions over a 10-week observation period following membrane implantation. The animals used were two male beagles. The experimental membrane, an insoluble hyaluronic acid membrane containing glycerin, was implanted under general anesthesia above the visceral pleura in the left pleural cavity of each dog after exposure to air for 30 min. A drainage tube was …

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Immunohistochemical Staining Of Peroxisome Proliferatoractivated Receptor Alpha And Gamma In Normal, Benign, And Malignant Canine Mammary Tissues, Wuthichai Klomkleaw, Promporn Raksaseri Sep 2018

Immunohistochemical Staining Of Peroxisome Proliferatoractivated Receptor Alpha And Gamma In Normal, Benign, And Malignant Canine Mammary Tissues, Wuthichai Klomkleaw, Promporn Raksaseri

The Thai Journal of Veterinary Medicine

The peroxisome proliferator-activated receptors (PPARs) belong to nuclear receptor superfamily acting as transcription factors related to lipid and glucose metabolism. There are 3 subtypes including PPARα, PPAR, and PPAR. PPARα and PPAR activations can inhibit tumor cell proliferation and differentiation in many cell types. However, the expressions of these receptors in canine mammary tissue are still unknown. This study was performed to investigate the expressions of PPARα and PPAR in normal, benign, and malignant canine mammary tissues. Twenty four bitches at the age of 5-15 years old, which undergone unilateral mastectomy, were used in the study. Mammary tissues were sectioned …

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Molecular Characterization Of Trichuris Ovis From Goats In Hunan Province Of China, Jia-Min Tang, Lu-Lin Song, Lin-Feng Nie, Fen Li Sep 2018

Molecular Characterization Of Trichuris Ovis From Goats In Hunan Province Of China, Jia-Min Tang, Lu-Lin Song, Lin-Feng Nie, Fen Li

The Thai Journal of Veterinary Medicine

Trichuris ovis (Nematoda: Trichuridae) is a widespread nematode that can infect a broad range of animal hosts, such as goats, sheep and oryx. This parasite is of vital importance as a pathogen, but its population genetics, molecular epidemiology and biology are still not well understood. In this study, T. ovis isolates were collected from goats in Hunan province, China, and sequences variation in cox1 gene were examined. A portion of cox1 gene from each collected adult T. ovis individual was separately amplified using the PCR method, and the amplicons were sequenced from both directions. The sequences of pcox1 gene were …

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Long-Term Effect Of Low-Dose Imatinib Therapy For Pulmonary Hypertension Due To Chronic Degenerative Mitral Valve Disease In Six Dogs, Zi Ping Leong, Shinji Arita, Yoshiaki Hikasa Sep 2018

Long-Term Effect Of Low-Dose Imatinib Therapy For Pulmonary Hypertension Due To Chronic Degenerative Mitral Valve Disease In Six Dogs, Zi Ping Leong, Shinji Arita, Yoshiaki Hikasa

The Thai Journal of Veterinary Medicine

Six dogs were diagnosed with pulmonary hypertension (PH) secondary to chronic degenerative mitral valve disease (CDMVD) on echocardiography. Imatinib (3 mg/kg, every 24 hours, PO) was initiated without any changes to the background therapy to treat the PH. Follow-up evaluations at 1, 3, 5 and 6 months revealed substantial clinical and hemodynamic improvements. One dog showed deterioration after the imatinib withdrawal to necessitate a restart therapy. No side effects were observed throughout the 6-month treatment course. Low-dose imatinib may provide a promising treatment alternative for CDMVD-associated PH in dogs.

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Ophthalmology Snapshot, Nalinee Tuntivanich Sep 2018

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

A nine-year-old, female Shih Tzu had presented with left buphthalmic eye. She had a history of chronic glaucoma (left eye), which had been treating with topical hypotensive medication for several months. Her right eye is normal.

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Comparison Of Dried Blood Spot, Buccal Swab, Cloacal Swab And Feces As Dna Sources To Identify Avian Sexes By Pcr, Sariya Asawakarn, Intuorn Teeranuwat, Natchaya Watcharaprapapong, Nutta Siriwatchaiporn, Panithan Somsai, Mullika Kuldee, Gunnaporn Suriyaphol, Sirakarnt Dhitavat Sep 2018

Comparison Of Dried Blood Spot, Buccal Swab, Cloacal Swab And Feces As Dna Sources To Identify Avian Sexes By Pcr, Sariya Asawakarn, Intuorn Teeranuwat, Natchaya Watcharaprapapong, Nutta Siriwatchaiporn, Panithan Somsai, Mullika Kuldee, Gunnaporn Suriyaphol, Sirakarnt Dhitavat

The Thai Journal of Veterinary Medicine

Absence of sexual dimorphism makes it difficult to determine bird’s sex. A number of both invasive and noninvasive methods as a DNA template of sampling for avian sexing can be used. The aim of this study was to compare the efficacy of different sources of DNA for avian sexing by PCR. Samples were collected from dried blood spots, buccal swabs, cloacal swabs and feces. The highest success rate for sexing determination was from the dried blood spots, accounting for 100%, while those from the buccal swabs, cloacal swabs and feces were 74%, 75.47% and 29.17%, respectively. The non-urate fecal samples …

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Comparison Of Gts And Rapd Assays To Characterize Thai Mycoplasma Gallisepticum Strains, Arithat Limsatanun, Jiroj Sasipreeyajan, Somsak Pakpinyo Sep 2018

Comparison Of Gts And Rapd Assays To Characterize Thai Mycoplasma Gallisepticum Strains, Arithat Limsatanun, Jiroj Sasipreeyajan, Somsak Pakpinyo

The Thai Journal of Veterinary Medicine

Mycoplasma gallisepticum (MG) is one of the important pathogens which have economic impact on the poultry industry worldwide. Molecular characterization is the effective method to study the relation among international strains and the epidemiology of MG transmission. In this study, 17 Thai MG strains were characterized by using 2 molecular assays including random amplified polymorphic DNA (RAPD) and gene-targeted sequencing (GTS) assays. Results showed that the RAPD assay could classify the Thai MG strains into 3 patterns.Most MG strains obtained from the same area were in the same pattern. Partial mgc2 gene was used to distinguish between the Thai MG …

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Monitoring Il-13 Expression In Relation To Mirna-155 And Mirna-133 Changes Following Intra-Tracheal Administration Of Mesenchymal Stem Cells And Conditioned Media In Ovalbuminsensitized Rats, Mahdi Ahmadi, Reza Rahbarghazi, Amir-Ali Shahbazfar, Rana Keyhanmanesh Sep 2018

Monitoring Il-13 Expression In Relation To Mirna-155 And Mirna-133 Changes Following Intra-Tracheal Administration Of Mesenchymal Stem Cells And Conditioned Media In Ovalbuminsensitized Rats, Mahdi Ahmadi, Reza Rahbarghazi, Amir-Ali Shahbazfar, Rana Keyhanmanesh

The Thai Journal of Veterinary Medicine

The overall goal of the current study was to show possibly mechanisms by which mesenchymal stem cells (MSCs) or conditioned media (CM) exert immune-modulatory properties for amelioration of asthmatic changes. Male rats were classified into healthy (C) and ovalbumin-sensitized groups (S), which further were divided into three subgroups (n=6); rats given intratracheally PBS (CPT and SPT groups), CM (CST and SST groups) and MSCs (CCT and SCT groups). Two weeks post treatment, IL-13, miRNA- 133 and miRNA-155 transcripts, pathological injuries and the homing of MSCs into the lung parenchyma were evaluated. The local administration of CM and notably MSCs blunted …

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E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai Sep 2018

E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai

The Thai Journal of Veterinary Medicine

The main purpose of the present study was to investigate the localization of epithelial cadherin (E-cadherin) in the oviduct and uterine horn of swamp buffalo at the follicular and mid-luteal phases. E-cadherin is a transmembrane glycoprotein which plays a vital role in the mechanism of the maintenance epithelial architecture, cell growth and proliferation, cell to cell adhesion including cell to epithelium adhesion. Female reproductive tracts of swamp buffaloes (n=30) were collected from local abattoirs and categorized into follicular (n=15) and mid-luteal (n=15) phases. Tissue samples from the oviducts and uterine horns were regularly handled according to histological and immunohistochemical techniques. …

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Therapeutic Use Of Sulfadimethoxine-Ormetoprim For Control Of Streptococcus Agalactiae Infection In Nile Tilapia (Oreochromis Niloticus) Fry, Nion Vinarukwong, Mintra Lukkana, Jan O. Berntsen, Janenuj Wongtavatchai Sep 2018

Therapeutic Use Of Sulfadimethoxine-Ormetoprim For Control Of Streptococcus Agalactiae Infection In Nile Tilapia (Oreochromis Niloticus) Fry, Nion Vinarukwong, Mintra Lukkana, Jan O. Berntsen, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

The therapeutic efficacy of sulfadimethoxine-ormetoprim (SDMX-OMP) against Streptococcus agalactiae (S. agalactiae) infection in tilapia (Oreochromis niloticus) was examined in tilapia fry. Fish (3+0.44 g) underwent 3-hour immersion challenge with 50% infective dose (2.58 x 106 CFU/ml) of S. agalactiae and were treated at 24 hours post challenge with daily dosages of 30 and 50 mg SDMX-OMP/kg body weight (BW) feeding medication for 7 days. The administration of medicated feed increased the survival of the infected tilapia fry. The survival rate of the challenged, non-medicated group was 51.25+4.79%. The fish which were challenged with S. agalactiae and received the 30 and …

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Decreasing Duration Of Androgenic Hormone Feeding Supplement For Production Of Male Monosex In Tilapia (Oreochromis Spp.) Fry, Nion Vinarukwong, Mintra Lukkana, Janenuj Wongtavatchai Sep 2018

Decreasing Duration Of Androgenic Hormone Feeding Supplement For Production Of Male Monosex In Tilapia (Oreochromis Spp.) Fry, Nion Vinarukwong, Mintra Lukkana, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

The effects of an exogenous androgenic hormone on sex differentiation were examined in Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis spp.) fry. Tilapia aquaculture in Thailand commonly applies methyltestosterone at the dose of 80 mg/kg diet on the first feeding of fish fry for 21-28 days to achieve male-monosex crop. In this study, male sex reversal was induced with an alternative androgenic hormone, 17α- methyldihydrotestosterone (MDHT), at a dose of 80 mg/kg diet for shorter periods; 5, 10, 15, or 20 days. Microscopic examination of fish gonad stained with aceto-carmine was used to determine male and female fish fry …

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