Veterinary Medicine Commons^™

Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp stem cell (hDPSC)-derived IPCs (hDPSC-IPCs) by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG- and ALGPA-encapsulated hDPSC-IPCs maintained viability for at least 336 hours and sustained …

Detection And Differentiation Of Haemosporidian Parasites In Chickens (Gallus Gallus Domesticus) Using Molecular Diagnostic Approach, Thi Xuan Mai Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Haemosporidioses in domestic chicken (Gallus gallus domesticus) are not only widely distribute but causing high mortality and non-specific symptoms. The multiplex PCR assay developed in this study can concurrently detect and differentiate four haemosporidian parasites Leucocytozoon caulleryi, Leucocytozoon sabrazesi, Plasmodium juxtanucleare, and Plasmodium gallinaceum. Comparing to microscopic examination performed in parallel on 157 samples, multiplex PCR assay exhibited significantly higher detection rate in diagnosis of haemosporidians (p = 0.000). The results showed that, L. sabrazesi, P. juxtanucleare, and P. gallinaceum detection rate of multiplex PCR were all higher than that of microscopic examination with p = 0.002, 0.000, and 0.004, …

Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aeromonas veronii outbreaks in tilapia farming caused relatively high mortality. Moreover, it was resistant to many kinds of antimicrobial used in Thailand aquaculture. According to no CLSI standard, the determination of antimicrobial efficacy has been limited phenotypically; the genomics study is required. This research aims to analyze the resistome of A. veronii isolated from diseased Tilapia in Chainat, Nong Khai and Uttaradit province, Thailand. Twelve isolates of A. veronii were identified base on gyrB sequencing then determined the MIC value to eight antimicrobials (AMP, AML, GEN, ENR, OXO, OXY, SXT, and FFC). According to MIC patterns, five representatives were performed …

Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Osteoarthritis (OA) is found secondary from medial patellar luxation (MPL) which is one of the most common diseases in small-breed dogs. Managements of the disease are surgical correction or a medical treatment. In the patients who are unable to do the surgical correction, non-steroidal anti-inflammatory drugs (NSAIDs) remains the drug of choice for OA therapy. Nowadays nutraceuticals have become more popular since there are no side effects once given for a long period of time. The purpose of this study was to evaluate the clinical chondroprotective effects of undenatured collagen type-II (UCII) on OA secondary to canine medial patellar luxation. …

Comparison Of The Physical Capacity Using 6-Minute Walk Test And Questionnaire Before And After Resection Of Elongated Soft Palate By Bipolar Sealing Device In French Bulldogs, Tamolwan Anyamaneecharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brachycephalic airway obstruction syndrome (BAOS) is an anatomical abnormality of upper airway commonly found in brachycephalic breeds leading to clinical signs and physical capacity impairment. The purposes of this study were to compare clinical signs and physical capacity in French bulldogs between before and after surgical correction using questionnaire and 6-minute walk test (6-MWT). Twenty-seven French bulldogs were enrolled in this study and divided into normal group (n=7) and BAOS group (n=20). History taking, clinical signs and questionnaire information were recorded and 6-MWT was performed in both groups. BAOS dogs had significantly higher severity of clinical signs from questionnaire with …

Serological Proteomic Screening And Evaluation Of A Recombinant Egg Antigen For The Diagnosis Of Low-Intensity Schistosoma Mansoni Infections In Endemic Area In Brazil, Vanessa Silva-Moraes, Lisa Marie Shollenberger, William Castro-Borges, Ana Lucia Teles Rabello, Donald A. Harn, Lia Carolina Soares Medeiros, Wander De Jesus Jeremias, Liliane Maria Vidal Siqueira, Caroline Stephane Salviano Pereira, Maria Luysa Camargos Pedrosa, Nathalie Bonatti Franco Almeida, Aureo Almeida, Jose Roberto Lambertucci, Nidia Francisca De Figueiredo Carneiro, Paulo Marcos Zech Coelho, Refaella Fortini Queiroz Grenfell

Biological Sciences Faculty Publications

Background

Despite decades of use of control programs, schistosomiasis remains a global public health problem. To further reduce prevalence and intensity of infection, or to achieve the goal of elimination in low-endemic areas, there needs to be better diagnostic tools to detect low-intensity infections in low-endemic areas in Brazil. The rationale for development of new diagnostic tools is that the current standard test Kato-Katz (KK) is not sensitive enough to detect low-intensity infections in low-endemic areas. In order to develop new diagnostic tools, we employed a proteomics approach to identify biomarkers associated with schistosome-specific immune responses in hopes of developing …

Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan

Animal and Food Sciences Faculty Publications

Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …

Kinetics Of The Chromosome 14 Microrna Cluster Ortholog And Its Potential Role During Placental Development In The Pregnant Mare, Pouya Dini, Peter Daels, Shavahn C. Loux, Alejandro Esteller-Vico, Mariano Carossino, Kirsten E. Scoggin, Barry A. Ball

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: The human chromosome 14 microRNA cluster (C14MC) is a conserved microRNA (miRNA) cluster across eutherian mammals, reported to play an important role in placental development. However, the expression kinetics and function of this cluster in the mammalian placenta are poorly understood. Here, we evaluated the expression kinetics of the equine C24MC, ortholog to the human C14MC, in the chorioallantoic membrane during the course of gestation.

Results: We demonstrated that C24MC-associated miRNAs presented a higher expression level during early stages of pregnancy, followed by a decline later in gestation. Evaluation of one member of C24MC (miR-409-3p) by in situ hybridization …

Hitting The Threshold Of Common Sense: The Time For Screening Limits To Guard Against Environmental Transfer Is Now, Clara Fenger, Thomas Tobin, Maria Catignani, Theodore Shults

Maxwell H. Gluck Equine Research Center Faculty Publications

Recreational drug use and the opiate epidemic have taken a great toll on the human population of the United States and beyond. Year over year, deaths from synthetic opioids—of which fentanyl ranks as the most prevalent—have skyrocketed, reaching 29,406 in 2017. Environmental transfer of drugs of human addiction to horses is nothing new. Cocaine and its primary metabolite, benzoylecgonine, have long been identified as environmental substances in post-race samples, and many jurisdictions have screening limits in place as a result. More recently, methamphetamine has begun to show up in post-race samples, reflecting the increasing addiction problem associated with this drug. …

Food Is Medicine, Katherine Keane

Senior Honors Projects

No abstract provided.

Presence Of Antibiotic Resistant Salmonella Spp. In Backyard Poultry And Their Environment, Nicole Land

Master's Theses

As keeping backyard poultry rises, human contact with zoonotic pathogens will increase. One such pathogen that backyard enthusiasts have exposure risks to is Salmonella spp. which may cause a potential public health threat due to its increasing multidrug resistancy. Salmonella spp. were present in 33 of 50 samples collected from 29 sites with backyard poultry coops in San Luis Obispo County during March to May in 2014. Two different Hardy-CHROME™ Salmonella Selective Media plates were used to culture and isolate positive samples of Salmonella spp.. Each positive isolate was tested for antimicrobial sensitivity to 6 standard antibiotics: Ampicillin, Bacitracin, …

Review Of The Cardiovascular Toxicity Of Amitriptyline Treatment For Canine Neuropathic Pain, Saikaew Sutayatram, Kumpanart Soontornvipart, Piyasiri Glangosol

The Thai Journal of Veterinary Medicine

Nowadays, the lifespan of pets has been increased by improvements in veterinary practice but age-related disorders are also increasing. Several nervous system attenuations are well-known to be the result of aging and/or pathological lesions. In many cases, neuropathic pain (NP) can develop and cause a significant impact on the clinical outcome and quality of life. With the difficulty in identification of the specific cause of NP, most NP management is planned according to clinical manifestations and recommended trial therapy. The drug of choice for trial therapy seems to be amitriptyline, a tricyclic antidepressant with multiple effects on neurotransmitter receptors and …

Comparison Of Isolation Techniques For Bone Marrow Derived Canine Mesenchymal Stem Cells (Mscs) And The Compatibility Of Mscs Loaded Onto Polycarpolactone Hydroxyapatite, Chalika Wangdee, Sasithorn Panasophonkul, Napaphat Thadavirul, Kumpanart Soontornvipart, Prasit Pavasant, Pitt Supaphol, Mongkol Techakumphu, Theerawat Tharasanit

The Thai Journal of Veterinary Medicine

Bone marrow was collected from 7 dogs and submitted to 3 different MSC isolation techniques (direct plating, red blood cell lysis and gradient density). The number of cells, expression of MSC markers and in vitro the osteogenic differentiation obtained from each technique were examined. In order to study in vivo the osteogenic capability of derived MSCs, non-union ulna lesions (n=3) were firstly induced by transplantation of a composite of polycarprolactone/ hydroxyapatite (PCL/HAp) scaffold experimentally on to a critical-size ulna bone defect. The MSCs were injected into the lesions. The non-union sites were examined by radiography, angiography and histology at 2, …

High Infection Rate Of Zika Virus In Mosquitoes Collected From An Area Of Active Zika Virus Transmission In Eastern Thailand, Apiwat Tawatsin, Atchara Phumee, Usavadee Thavara, Patcharawan Sirisopa, Wanapa Ritthison, Kitsanaphong Thammakosol, Proawpilart Intayot, Yutthana Joyjinda, Supaporn Wacharapluesadee, Thiravat Hemachudha, Padet Siriyasatien

The Thai Journal of Veterinary Medicine

Zika virus (ZIKV), chikungunya virus (CHIKV) and dengue virus (DENV) are emerging and re-emerging arboviral diseases. These viruses are transmitted to humans through the bites of Aedes mosquitoes. Recently, ZIKV infection has been described as an emerging disease in Thailand and many countries, especially in tropical and subtropical areas. Specific drugs and vaccines against these infections are unavailable; therefore, effective disease control relies on vector control measures only. To understand the transmission cycle of these viruses and mosquito vectors, this study is designed to investigate the natural infection of ZIKV, CHIKV and DENV in field-caught mosquitoes by molecular techniques. Adults …

Computed Tomographic Contrast Enhancement And Tumor Angiogenesis In Canine Oral Tumors, Urapa Klansnoh, Wijit Banlunara, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

The potential value of bolus tracking contrast enhanced computed tomography (CT) in the diagnosis of oral tumors in dogs was investigated by examining the relationship between contrast-enhanced CT image density and microvascularization. Among 20 dogs with oral tumors, aged 8 – 17 years, weight 3.6 – 40.0 kg, 13 dogs were suffering with melanoma (MM), 4 with squamous cell carcinoma (SCC), and 3 with fibrosarcoma. Based on contrast-enhanced CT scan, the time lag from the injection of contrast medium into the right cephalic vein until the contrast medium returned to the mid-cervical external jugular vein at the level of the …

A Preliminary Study On Diversity Of Midgut Microbiota In Aedes Aegypti (Linnaeus) And Culex Quinquefasciatus (Say) Collected From Bangkok, Thailand, Sonthaya Tiawsirisup, Jirinnut Sirijutalak, Mananya Sondang, Manunya Supol, Nut Ansusinha, Ranida Tuanudom, Channarong Rodkhum

The Thai Journal of Veterinary Medicine

Aedes aegypti and Culex quinquefasciatus are important mosquito vectors for many infectious diseases. A number of factors affect the vector competence of these mosquitoes for a specific pathogen. The bacteria harbored in the midgut are known to influence mosquito physiology and can alter the response to various pathogens. Bacteria from the midgut of Ae. aegypti and Cx. quinquefasciatus were cultured and identified using bacteriological and molecular techniques in this study in which two groups of mosquitoes were examined. The first group was laboratory- reared, and the second group were field- collected mosquitoes from Bangkok. Twelve bacterial genera ( i. e. …

Survival Time And Prognosis Factors In Hypertrophic Cardiomyopathy Cats With Congestive Heart Failure, Lalida Tantisuwat, Panduangjai Puangampai, Pornchanok Panpakdee, Thitinan Tangarsasilp, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

Hypertrophic cardiomyopathy (HCM) is a common myocardial disease in cats. Nowadays, there is no study determining the survival time of cats affected with HCM in Thailand. This study aimed to evaluate the survival time in HCM cats with congestive heart failure (CHF) in Thailand and to determine the factors associating with survival time. A retrospective study was performed in 47 HCM cats to analyze survival time and the association of survival time and factors including sex, age, breeds, weight, physical findings, the presence of complications, medication and echocardiographic findings. The data was analyzed by the Log-Rank test and displayed with …

Effectiveness Of Sentinel Rodents For Surveillance Of Exposure To Undocumented Bacterial Pathogens In Animal Research Facility, Shih-Keng Loong, Haryanti-Azura Mohammad-Wali, Nurul-Asma-Anati Che-Mat-Seri, Nur-Hidayana Mahfodz, Dzuzaini Mohd-Ghazali, Pooi-Fong Wong, Sazaly Abubakar

The Thai Journal of Veterinary Medicine

The use of sentinel animals in animal research facilities is the cornerstone of animal health monitoring for exposure to natural pathogens of laboratory animals. This is because infection with these pathogens produces no overt signs of disease yet the infection may affect the outcome of research utilising these animals. One of the important pathogens includes Klebsiella pneumoniae, whose prevalence can be high in laboratory animals. This study sought to document the prevalence of K. pneumoniae and other undocumented bacterial pathogens in laboratory rodents for a 3- year period. K. pneumoniae and Chryseobacterium gleum were isolated from sentinel ICR mice housed …

Surgical Treatment For Tubular Colonic Duplication Communicating To Retroperitoneal Space In An Adult Dog, Nithida Boonwittaya, Piyathip Choochalermporn, Sunee Kunakornsawat, Teerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

Canine colonic duplication is a rare congenital anomaly, usually symptomatic in the first year of life, with the common signs of bowel obstruction. Most importantly, colonic duplication in an adult dog has not been reported. This case document reports a non-communicating tubular colonic duplication with concurrent retroperitoneal mass in a 3- year-old female crossbred dog, presented for further diagnosis and treatment of the abdominal mass. Without any remarkable clinical signs, physical examination noted only abdominal distension with mild discomfort. Ultrasonography demonstrated a large mass in the mid-caudal abdomen, a tubular mass appearance on the colonic wall and the presence of …

Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram

The Thai Journal of Veterinary Medicine

A 10-year-old female Pomeranian dog was presented to the Small Animal Teaching Hospital, Chulalongkorn University, on December, 2018, with a history of anorexia, frequent vomiting, depress and oliguria. Physical examination revealed that the dog was depressed but responsive to the stimuli. The dog was dehydrated (7%) with pale-pink mucous membrane. The capillary refilling time of 1.5 seconds with strong femoral pulse were detected. The respiratory rate was 65 breath per minute with normal sound while heart rate was approximately 60 beats per minute without cardiac murmur from auscultation using stethoscope. The systolic blood pressure was 120 mmHg. The blood collection …

What Is Your Diagnosis?, Ninlawan Thammasiri, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

A thirteen kilograms, 6-year-old, male, Shih Tzu dog.

Small Dosages Of Alpha1 Adrenoceptor Antagonist On Goat Semen Quality And Seminal Fluid Volume, Sakdichod Kimsakulvech, Sookruetai Boonmasawai, Dulyatud Gronsang, Chowalit Nakthong, Yupaporn Lanamtiang

The Thai Journal of Veterinary Medicine

The frozen goat semen process must wash seminal fluid from goat semen. A small dosage of alpha1 adrenoceptor antagonist or tamsulosin may reduce seminal fluid in goat. A latin square 4x4 was applied to twelve male goats to receive either normal saline (NSS), tamsulosin 3, 6 and 9 µg/kg intramuscular injection at one-week intervals. Semen collection by artificial vagina and libido scoring was performed within one-hour post injection. Semen quality and seminal fluid were evaluated after semen collection The results showed that none of the libido scores in the groups was different. Anejaculations occurred in 1 (8.3%), 3 (25%) and …

Effects Of Supplementing Varying Levels Of 1,3-Diacylglycerol (Dag) On Growth Performance, Apparent Nutrient Digestibility And Selected Fecal Microbial Populations In Growing Pigs, Wen Chao Liu, Kwan Sik Yun, In Ho Kim

The Thai Journal of Veterinary Medicine

The objective of the present study was to investigate the effects of 1,3-diacylglycerol (DAG) supplementation on growth performance, apparent digestibility and selected fecal microbial populations in growing pigs. A total of 80 crossbred pigs [(Landrace × Yorkshire) × Duroc] with an average initial body weight (BW) of 27.52 ± 0.20 kg were used in a 6 week feeding trial. Pigs were allotted to 4 dietary treatments based on their initial BW and sex (5 replications; 4 pigs per pen with 2 barrows and 2 gilts). Dietary treatments included: 1) CON, basal diets; 2) CON+0.075% 1,3-DAG; 3) CON+0.10% 1,3-DAG; 4) CON+0.15% …

Quantitative Microbial Risk Assessment For Parts, Ground, And Msc Poultry Product Including Intervention Analysis And Exploration Of Enterobacteriaceae As An Indicator Organism In Poultry Processing, Leigh Ann Parette

Graduate Theses and Dissertations

Samples collected at five different large bird poultry processing facilities over a period of 7 months from prescald to post debone locations were enumerated for Enterobacteriaceae, Salmonella spp., and Campylobacter spp. and the results were used to create Quantitative Microbial Risk Analyses (QMRA) models for parts, ground, and mechanically separated chicken (MSC) products. Sensitivity analyses indicated the points in the process at which reductions would be most advantageous to the endpoint and simulation models were run to test reductions required to meet the current USDA performance standards.

These data were analyzed to determine the reductions from one node (location) to …

Anti-Proliferative And Total Erk1/2 Inhibitory Effects Of Plant Flavonols On Human Cervical Cancer (Hela) Cells, Sookruetai Boonmasawai, Ladawan Sariya, Arpron Leesombun, Kridsada Chaichoun, Jarupha Taowan, Orathai Thongjuy

The Thai Journal of Veterinary Medicine

Kaempferol, myricetin and quercetin are abundant flavonols in edible fruit and vegetables. Much previous data has shown the beneficial effects of these flavonols in cancer treatments. Thus, the cytotoxic effects on rhesus monkey kidney epithelial cells (LLC-MK2) and the anti-proliferative effects on human cervical cancer (HeLa) cells through total ERK protein expression of kaempferol, myricetin and quercetin have been investigated. The cytotoxic assay at 24h revealed the high safety of three flavonols on LLC-MK2 cells. Kaempferol (1-1000 µM) did not have any significant effect on the viability of these normal epithelial cells. The cytotoxicity of myricetin and quercetin was 5 …

Hemostatic Efficacy Of Sheep-Derived Fibrin Glue For Liver Biopsy In Swine, Siriluck Luckanahasaporn, Theerawat Tharasanit, Passakorn Briksawan, Sumit Durongphongthron

The Thai Journal of Veterinary Medicine

Fibrin glue has been widely used for controlling hemorrhage and sealing tissue in surgery. The purpose of this study was to evaluate the hemostatic efficacy of sheep-derived fibrin glue. Six Marino sheep were used to collect concentrate fibrinogen preparation by the ammonium sulfate precipitation method. Crossbred pigs (n=6) were used to evaluate hemostatic efficacy by monitoring whole blood clotting time by the glass slide method, bleeding time and bleeding quantity during liver biopsy. Mean whole blood clotting time on a glass slide started at 201.1±90.47 and 4.43±3.73 seconds, and was completed at 447.83±63.77 and 31.93±4.28 seconds in control and treatment …

The Potential Use Of Phytoestrogen Containing The Herb, Pueraria Mirifica, For Bone Healing In Osteoporotic Monkeys, Donlaporn Kittivanichkul, Nan Choisunirachon, Kumpanart Soontornvipart, Suchinda Malaivijitnond

The Thai Journal of Veterinary Medicine

Within the last decade, the positive effects of Pueraria mirifica on osteoporosis have been established in rats and monkeys. However, the effect of P. mirifica on bone healing has not been elucidated. In this study, post-menopausal osteoporotic monkeys were subjected to an iliac crest biopsy, divided into two groups (5 animals/group), and fed daily with standard monkey diet alone (PMP0 group) or mixed with 1,000 mg/kg body weight of P. mirifica powder (PMP1000 group) for 16 months. The progression of the bone healing was continuously assessed by X-ray radiography, and three individuals from each group were selected for a computed …

Comparison Of Sperm Freezability And Fertility Among Hmong Black-Bone, Barred Plymouth Rock And Thai Native (Pradu Hang Dam) Chickens, Saennamphung Somtaw, Pornjit Sonseeda, Nalinee Tubtimtong, Yupin Phasuk, Thevin Vongpralub

The Thai Journal of Veterinary Medicine

Semen cryopreservation is the most useful method for the long-term storage of chicken genetic resources. The objective of this study was to compare post- thaw semen quality of Hmong Black- Bone to Barred Plymouth Rock and Thai native ( Pradu Hang Dam) chickens in terms of sperm motility, viability and fertility. A total of 9 males of each breed were used; semen collected from each cock was subsequently evaluated under a microscope. Semen samples were diluted with Schramm diluent, cooled down from 20– 25oC to 5oC and diluent plus DMF ( dimethylformamide) was added (6% DMF of final volume when …

Efficacy Of Different Vaccination Programs Of Recombinant Hvt-Ndv Vaccine Against Genotype Vii Ndv Challenge In Broiler Chickens, Nataya Charoenvisal, Bubpa Supannamoke, Rik Koopman, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This study determined the efficacy of recombinant HVT- NDV ( rHVT- NDV) vaccine simultaneously vaccinated with live vaccine at 1 day old and a booster vaccination with live vaccine at 10 days old against Asiatic, Genotype VII NDV in broiler chickens. The chickens were divided into 5 groups. The chickens in groups 1, 2 and 3 were vaccinated with rHVT- NDV vaccine and different live vaccines at 1 day old, and then finally received a booster vaccination at 10 days old. The chickens in groups 4 and 5 served as the positive and negative control groups, respectively. The chickens in …

Plasmid Profile Of Enterococcus Faecium And Enterococcus Faecalis Isolated From Pigs, Pork And Humans In Thai-Laos Border Provinces, Wink Phyo Thu, Nuananong Sinwat, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

This study aimed to determine plasmid profile of E. faecium and E. faecalis isolated from pigs, pig products and humans in Thailand-Laos border provinces. A total of 96 Enterococcus spp. including E. faecium (n=59) and E. faecalis (n=37) from pigs, pork and humans were included. All were determined for their antimicrobial susceptibilities and plasmid profile. Most isolates (96.9%) carried one to four plasmids with a molecular weight of 0.03-35 kb in E. faecium and 19-40 kb in E. faecalis. The 26 kb plasmid was most commonly detected. Variable plasmid profiles were defined, of which the most common pattern was, 23 …