Open Access. Powered by Scholars. Published by Universities.®

Veterinary Medicine Commons

Open Access. Powered by Scholars. Published by Universities.®

Discipline
Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 2071 - 2100 of 9758

Full-Text Articles in Veterinary Medicine

Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li Sep 2020

Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li

The Thai Journal of Veterinary Medicine

Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and …


Repairing Of Rabbit Calvarial Defects By Rapid Prototyping Bisgma And Hydroxy-Appatite Incorporated Bisgma, Naris Thengchaisri, Chaiyakorn Thitiyanaporn, Siriporn Tanodekaew Sep 2020

Repairing Of Rabbit Calvarial Defects By Rapid Prototyping Bisgma And Hydroxy-Appatite Incorporated Bisgma, Naris Thengchaisri, Chaiyakorn Thitiyanaporn, Siriporn Tanodekaew

The Thai Journal of Veterinary Medicine

The in vivo bone regenerative capacity and biocompatibility of bisphenol A glycidylmethacrylate (BisGMA) implants fabricated by Stereolithography Rapid Prototyping machine (SLA) wasinvestigated in rabbit’s calvarial defect model. The study focused on two different materials: BisGMA alone and hydroxyapatite (HA) incorporated BisGMA, of which were formulated especially for SLA fabrication. Each rabbit’s skull implant was fabricated with size 1x1 cm2according to the rabbit skull’s contour based on its Computed Tomographic dataand replaced at the full thickness cavarial defects in 6 rabbits. Computed Tomography (CT) scan and blood tests were performed at certain time points during 6 months after surgical operation to …


Molecular Characterization Of Equine Herpesvirus-1 And Asinine Herpesvirus -5 Isolated From Aborted Fetuses Of Arabian Horses, Rabab Taha Hassanien, Ehab Mostafa El-Nahas, Nahed Ahmed Mahmoud, Gabr Fikry El-Bagoury Sep 2020

Molecular Characterization Of Equine Herpesvirus-1 And Asinine Herpesvirus -5 Isolated From Aborted Fetuses Of Arabian Horses, Rabab Taha Hassanien, Ehab Mostafa El-Nahas, Nahed Ahmed Mahmoud, Gabr Fikry El-Bagoury

The Thai Journal of Veterinary Medicine

Infections with equine herpesviruses(EHVs) are widespread in equine populations worldwide. Whilst EHV-1 produces well-documented reproductive losses in equids, the role of asinineherpesviruses-5 (AHV-5) to induce abortion is still enigmatic. In the present study, EHV-1 and AHV-5 were detected in aborted fetuses of Arabian horses in Egypt by consensus nested PCR of DNA polymerase gene, with a trial for viral isolation on Rabbit Kidney 13 cell line (RK-13) and identification by indirect immunofluorescence. The partial analysis of DNA polymerase gene sequence revealed that the Egyptian strains EHV-1 and AHV-5 were related to Australian origin EHV-1 838/89 (Accession. no, KF434370) and Austrian …


Analyses Of The Expression, Immunohistochemical Properties And Serodiagnostic Potential Of Schistosoma Japonicum Peroxiredoxin-4, Minh-Anh Dang-Trinh, Jose Ma. M. Angeles, Kharleezelle J. Moendeg, Adrian Miki C. Macalanda, Thu-Thuy Nguyen, Luna Higuchi, Shotaro Nakagun, Masahi Kirinoki, Yuichi Chigusa, Yasuyuki Goto, Shin-Ichiro Kawazu Sep 2020

Analyses Of The Expression, Immunohistochemical Properties And Serodiagnostic Potential Of Schistosoma Japonicum Peroxiredoxin-4, Minh-Anh Dang-Trinh, Jose Ma. M. Angeles, Kharleezelle J. Moendeg, Adrian Miki C. Macalanda, Thu-Thuy Nguyen, Luna Higuchi, Shotaro Nakagun, Masahi Kirinoki, Yuichi Chigusa, Yasuyuki Goto, Shin-Ichiro Kawazu

Biology Faculty Publications

Background

Schistosoma japonicum, which inhabits the mesenteric vein of the mammalian hosts for about 20 to 30 years, is subjected to the oxidative stresses from the host defense mechanism during their intra-mammalian stages. To counteract this host immune attack, the parasite utilizes their antioxidant system for survival inside the host. Peroxiredoxins (Prxs), thiol-specific antioxidant proteins, play an essential role for protecting the parasite against oxidative stress by reducing hydrogen peroxide to water. Only three types of 2-Cys Prxs have been previously characterized in S. japonicum whereas a fourth Prx has been identified for Schistosoma mansoni as Prx-4. A sequence …


The Effects Of Water Submersion On Cattle Ticks, Atchara Artchayasawat, Parichart Boueroy, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Pranee Sriraj, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Opal Pitaksakulrat, Panaratana Ratanasuwan, Apiporn Suwannatrai, Chatanun Eamudomkarn, Porntip Laummaunwai, Wu Zhiliang Sep 2020

The Effects Of Water Submersion On Cattle Ticks, Atchara Artchayasawat, Parichart Boueroy, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Pranee Sriraj, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Opal Pitaksakulrat, Panaratana Ratanasuwan, Apiporn Suwannatrai, Chatanun Eamudomkarn, Porntip Laummaunwai, Wu Zhiliang

The Thai Journal of Veterinary Medicine

Rhipicephalus (Boophilus)microplusis an important vector transmitting hemoparasitesin cattle and is, therefore, the cause of significant loss to cattle production. The control of ticks with synthetic acaricides is partially successful. However, parasite resistance to these compoundshas been reported.This study aimedto evaluate the effects of water submersion on cattle ticks. Ticks were divided into three weight classes[small (3-10 mg),medium (10-30 mg)and large(>70 mg)].All ticks in the small weight class died after immersion in water for as little as 5 mins. Some ticks in the medium weight class died after immersion for10 and 15 minsbutnone in the largest weight class died at …


Cardiac Myxoma In The Pulmonary Trunk Of A Canine: Presence Of Mesenchymal Stem-Like Cells, Luis Alfaro, Alexis Enciso, Javier Enciso Sep 2020

Cardiac Myxoma In The Pulmonary Trunk Of A Canine: Presence Of Mesenchymal Stem-Like Cells, Luis Alfaro, Alexis Enciso, Javier Enciso

The Thai Journal of Veterinary Medicine

Cardiac myxoma is a rare disease in dogs. Its histogenic origin has not yet been established, but the involvement of mesenchymal stem cells has been suggested in humans. Here we report an encapsulated and benign cardiac myxoma located at the entrance to the pulmonary trunk of the heart of an 8-year-old male Yorkshire Terrier dog presenting sudden death in the absence of clinical symptoms related to cardiac alterations. Hematoxylin/eosin staining demonstrated the mixoid origin of the myxoma, showing abundant hypocellular stroma with polygonal cells, while immunohistochemistry (IHC) corroborated its mesenchymal lineage with the detection of vimentin and α-SMA positive cell …


Antiviral Evaluation Of Bioactive Azo Derivatives To Treat Endemic Poultry Viruses, Tehreem Tahir, Muhammad Ashfaq, Mirza Imran Shahzad, Rukhsana Tabassum Sep 2020

Antiviral Evaluation Of Bioactive Azo Derivatives To Treat Endemic Poultry Viruses, Tehreem Tahir, Muhammad Ashfaq, Mirza Imran Shahzad, Rukhsana Tabassum

The Thai Journal of Veterinary Medicine

The present study focused on the synthesis, In Ovoantiviral evaluation and In silicostudy of most active antiviral compounds of the azo series. The synthesis of title compounds was done by the coupling reaction of diazonium salt solutions withactive methylene (1,3-dioxolane and benzimidazole), to yield [(E)-1-(1,3-dioxolan-2-yl)-2-phenyldiazene] (A1), [(E)-1-(1,3-dioxolan-2-yl)-2-(4-methyl-phenyl)diazene] (A2), 2-[(E)-phenyldiazenyl]-1H-benzimidazole] (A3) , [(E)-1-(1,3-dioxolan-2-yl)-2-(4-ethylphenyl)diazene] (A4) and [(E)-1-(1,3-dioxolan-2-yl)-2-(2-methylphenyl)diazene] (A5). The structures of newly synthesized molecules were elucidated by spectroscopic techniques (EI-MS and FT-IR). In Ovoscreening of compounds against avian influenza virus (AIV) H9N2 strain and Newcastle Disease virus (NDV) Lasota strain was done. The evaluation data suggested that azo compound (A5) exhibited the …


Cryopreservation Of Boar Semen: Where We Are, Panida Chanapiwat, Kampon Kaeoket Sep 2020

Cryopreservation Of Boar Semen: Where We Are, Panida Chanapiwat, Kampon Kaeoket

The Thai Journal of Veterinary Medicine

The preservation of boar semen can be divided intotwo methods as follows: fresh boar semen and frozen boar semen. The first method is only to preserve the sperm for a short period, ranging from 3 to 10 days and depends on the semen extender used, however, its benefit is only in use for artificial insemination on a particular farm or for a short distant transportation. The latter method has been developed in order to keep the semen of superior genetic boars for long distant transportation or preservation of their genetics for future production or to set upa new herd in …


Effects Of Systemic Administrations Of Medetomidine And Xylazine On Ex Vivo Platelet Aggregation In Clinically Normal Cats, Takuya Matsukawa, Yoshiaki Hikasa Sep 2020

Effects Of Systemic Administrations Of Medetomidine And Xylazine On Ex Vivo Platelet Aggregation In Clinically Normal Cats, Takuya Matsukawa, Yoshiaki Hikasa

The Thai Journal of Veterinary Medicine

This study aimed to investigate and compare the effects of systemic administration of medetomidine and xylazine on ex vivo platelet aggregation inclinically normal cats.Five cats were repeatedly used in each of 7groups.The cats received saline as the non-medicated control; 0.5, 2.0, and 4.0 mg/kg xylazine; and 20, 80, and 160 μg/kg BW medetomidine intramuscularly.Venous blood was collected 4 times (0, 2, 4, and 7 h) after injection of both agents and platelet-rich plasma was prepared. Ex vivo percent platelet aggregationwas determined via a turbidimetric method. Collagen and adenosine diphosphate (ADP) were used to initiate aggregation. Administrations of xylazine at all …


Biosecurity Practices In Commercial And House Hold Poultry Farms In The Delta Region, Egypt: I-Correlation Between Level Of Biosecurity And Prevalence Ofpoultry Mites, Fatma Abouelenien, Maha Eleisway, Ismail Elshahawy, Samy Almidany, Nagham Elsaidy Sep 2020

Biosecurity Practices In Commercial And House Hold Poultry Farms In The Delta Region, Egypt: I-Correlation Between Level Of Biosecurity And Prevalence Ofpoultry Mites, Fatma Abouelenien, Maha Eleisway, Ismail Elshahawy, Samy Almidany, Nagham Elsaidy

The Thai Journal of Veterinary Medicine

The risk of parasitic disease outbreaks among commercial poultry farms can result in significant economic loss for the farmer and the integration. These diseases can be reduced by the proper application of biosecurity measures. This study primarily aimed at assessing the current biosecurity practices applied in different kinds of poultry farm (broiler, layer, duck and mixed farms) inKafrelshiekh Governorate (Delta region), Egypt.Correlating the compliance of the studied biosecurity measures with the prevalence of poultry mites in the surveyed farms was the second aim. For achieving these aims, on-farm questionnaire surveys and observational studies were conducted from January 2017 to August …


Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwannachai, Kumpanart Soontornvipart Sep 2020

Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwannachai, Kumpanart Soontornvipart

The Thai Journal of Veterinary Medicine

Osteoarthritis (OA) is found secondary to medial patellar luxation (MPL) which is one of the most common diseases in small-breed dogs. Management of the disease is surgical correction or medical treatment. In patients who are unable to do the surgical correction, non-steroidal anti-inflammatory drugs (NSAIDs) remain the drugs of choice for OA therapy. Nowadays nutraceuticals have become more popular since there are no side effects when given for along period. The purpose of this study was to evaluate the clinical chondroprotective effects of undenatured collagen type-II (UCII) on osteoarthritis secondary to canine medial patellar luxation. Nine small-breed dogs (thirteen stifle …


Clinical Assessment Of Brachycephalic Airway Obstructive Syndrome Using Questionnaire And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Sumit Durongphongtorn, Pasakorn Brikshavana Sep 2020

Clinical Assessment Of Brachycephalic Airway Obstructive Syndrome Using Questionnaire And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Sumit Durongphongtorn, Pasakorn Brikshavana

The Thai Journal of Veterinary Medicine

Brachycephalic airway obstructive syndrome (BAOS) is an anatomical abnormality of the upper airway commonly found in brachycephalic breeds leading to clinical signs and physical capacity impairment. The purposes of this study were to objectively assess the severity of the clinical signs and physical capacity in French Bulldogs with BAOS using questionnaire and 6-minute walk test (6-MWT), respectively. A total of 26 French Bulldogs were enrolled in this study and divided into a normal group (n = 7), a moderate BAOS group (n = 8) and a severe BAOS group (n = 11). After history taking and physical examination, the questionnaire …


Isolation And Identification Of Klebsiella Aerogenes From Bee Colonies In Bee Dysbiosis, Oleksandr Galatiuk, Tatiana Romanishina, Anastasiia Lakhman, Olga Zastulka, Ralitsa Balkanska Sep 2020

Isolation And Identification Of Klebsiella Aerogenes From Bee Colonies In Bee Dysbiosis, Oleksandr Galatiuk, Tatiana Romanishina, Anastasiia Lakhman, Olga Zastulka, Ralitsa Balkanska

The Thai Journal of Veterinary Medicine

This article presents modern methods for obtaining and isolatingpure culture from bee colonies (from honeycombswith infected broodsand contaminated by faeces of bees), and the identification and indication of pathogenic bacteria of Klebsiellaaerogenes species in honey beesduring the winter, spring,summer andthe autumn. The purposewas to isolate and identifythe pure culture of enterobacteria from the pathological material of diseased bee colonies. The study of their basic biological properties for the possible use of the obtained strains, which should be used as indicator bacterial cultures in the study of the biological properties of drugs for the treatment and prevention of enterobacteriosis of beeswas …


Production Of Immunoglobulin Y Against Dog Erythrocyte Antigen (Dea) 1.1 From Egg Yolk, Noppadol Prasertsincharoen, Pornphimon Metheenukul Sep 2020

Production Of Immunoglobulin Y Against Dog Erythrocyte Antigen (Dea) 1.1 From Egg Yolk, Noppadol Prasertsincharoen, Pornphimon Metheenukul

The Thai Journal of Veterinary Medicine

The dog erythrocyte antigen (DEA) as a blood group antigen in dogs, especially DEA 1.1, has the highest immunogenicity. IgYis an excellent antibody for use in immunological assays involving mammalian sera. The aim of this study was to produce and purify IgY against DEA1.1 from chicken egg yolks for canine blood group detection. Hens were immunized by intramuscular vaccination. IgY was achieved after three booster immunizations by DEA 1.1-positive canine ghost cells. The extraction of total IgY from the egg yolk was carried out using precipitation by polyethylene glycol 6000 and dialysis. The purified protein and yield of IgY were …


Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici Sep 2020

Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici

The Thai Journal of Veterinary Medicine

In this study, the possible protective effect of red radish containing total phenolic content and antioxidant components on histopathological and immuno histochemical active caspase-3, TNF-α and Iba-1, serum ALT activity, triglyceride, total cholesterol, LDL-cholesterol, liver malondialdehyde (MDA) and nitric oxide (NO) levels were evaluated in CCl4induced liver injury in Wistar rats. The rats were randomly divided into six groups (n= 12 to each group). The first group was used as a control which was given only intraperitoneal 0.9% NaCl (0.2 mL / kg / bw); while fermented red radish (FRR) at a dose of 250 mg/kg or 500 mg/kg was …


Preparation Of Swine Isg15 Polyclonal Antibody And Its Application In Classical Swine Fever Virus, Xin-Bin Cai, Hua-Lin Hou, Li-Yuan Zhang, Rong-Ze Tang, Shan Yin, Xiao-Quan Li, Ke Zhang, Qian-Nan Zhao, Xue Ying, Li-Juan Su, Shi-Kai Sun, Xiao-Ning Li, Ting Rong Luo Sep 2020

Preparation Of Swine Isg15 Polyclonal Antibody And Its Application In Classical Swine Fever Virus, Xin-Bin Cai, Hua-Lin Hou, Li-Yuan Zhang, Rong-Ze Tang, Shan Yin, Xiao-Quan Li, Ke Zhang, Qian-Nan Zhao, Xue Ying, Li-Juan Su, Shi-Kai Sun, Xiao-Ning Li, Ting Rong Luo

The Thai Journal of Veterinary Medicine

Interferon-stimulated gene 15 (ISG15) is known to participate in innate and adaptive immune responses against viral infections. Classical swine fever virus (CSFV) causes a global, economically important and highly contagious disease in pigs. To explore theinteraction between ISG15 and CSFV, the ISG15gene was amplified, cloned and expressed in E. coliBL21 cells. The expressed ISG15 protein was purified by Ni-NTA agarose column and used to prepare a polyclonal mouse antibody. The anti-ISG15 antibody produced in mice could react with ISG15 expressed in the prokaryotic BL-21 cells as well as natural swine ISG15 expressed in PK-15 cells. This polyclonal antibody against swine …


Correction Of Diaphragmatic Hernia Using The Transverse Colon In A Dog, Dongwook Kim, Gonhyung Kim Sep 2020

Correction Of Diaphragmatic Hernia Using The Transverse Colon In A Dog, Dongwook Kim, Gonhyung Kim

The Thai Journal of Veterinary Medicine

A 3-year-old female Golden Retriever was referred with weakness, tachypnea and dyspnea. An incomplete diaphragmatic silhouette, gas-filled small intestine and pleural effusion in the thoracic cavitywere revealed in radiographs, implying a diaphragmatic hernia. After laparotomy, the abdominalstructureswere gently retrieved into the abdomen and a defect of 2×7 cm2was found. The transverse colonwas used as an autologous tissue flap for the repair of the defect. There were no complications identified for 2 years after surgery. Thus, patching with the transverse colon can be considered as an alternative for treating a defect in patients with a diaphragmatic hernia.


Vampire Bats: Preparing For Range Expansion Into The U.S., Michael J. Bodenchuk, David L. Bergman Aug 2020

Vampire Bats: Preparing For Range Expansion Into The U.S., Michael J. Bodenchuk, David L. Bergman

United States Department of Agriculture Wildlife Services: Staff Publications

The common vampire bat apparently is expanding its range northwards in Mexico and seems poised to enter the southern United States. Climate models predict suitable habitat in the U.S. in south Texas and parts of southern Arizona. While vampire bats’ northward range expansion is not unexpected, the fact that this species brings a strain of rabies that impacts livestock and people warrants a strategic response. Annual economic damages from bats are estimated between $7M and $9M, largely associated with deaths of livestock from rabies. To prepare for the emerging rabies issue, USDA Wildlife Services programs in Texas and Arizona have …


The P-Glycoprotein Repertoire Of The Equine Parasitic Nematode Parascaris Univalens, Alexander P. Gerhard, Jürgen Krücken, Emanuel Heitlinger, I. Jana I. Janssen, Marta Basiaga, Sławomir Kornaś, Céline Beier, Martin K. Nielsen, Richard E. Davis, Jianbin Wang, Georg Von Samson-Himmelstjerna Aug 2020

The P-Glycoprotein Repertoire Of The Equine Parasitic Nematode Parascaris Univalens, Alexander P. Gerhard, Jürgen Krücken, Emanuel Heitlinger, I. Jana I. Janssen, Marta Basiaga, Sławomir Kornaś, Céline Beier, Martin K. Nielsen, Richard E. Davis, Jianbin Wang, Georg Von Samson-Himmelstjerna

Maxwell H. Gluck Equine Research Center Faculty Publications

P-glycoproteins (Pgp) have been proposed as contributors to the widespread macrocyclic lactone (ML) resistance in several nematode species including a major pathogen of foals, Parascaris univalens. Using new and available RNA-seq data, ten different genomic loci encoding Pgps were identified and characterized by transcriptome-guided RT-PCRs and Sanger sequencing. Phylogenetic analysis revealed an ascarid-specific Pgp lineage, Pgp-18, as well as two paralogues of Pgp-11 and Pgp-16. Comparative gene expression analyses in P. univalens and Caenorhabditis elegans show that the intestine is the major site of expression but individual gene expression patterns were not conserved between the two nematodes. In P. …


An Evaluation Of Bird And Bat Mortality At Wind Turbines In The Northeastern United States, Daniel Y. Choi, Thomas W. Wittig, Bryan M. Kluever Aug 2020

An Evaluation Of Bird And Bat Mortality At Wind Turbines In The Northeastern United States, Daniel Y. Choi, Thomas W. Wittig, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

Wind energy offers substantial environmental benefits, but wind facilities can negatively impact wildlife, including birds and bats. Researchers and managers have made major efforts to chronicle bird and bat mortality associated with wind facilities, but few studies have examined the patterns and underlying mechanisms of spatial patterns of fatalities at wind facilities. Understanding the horizontal fall distance between a carcass and the nearest turbine pole is important in designing effective search protocols and estimating total mortality. We explored patterns in taxonomic composition and fall distance of bird and bat carcasses at wind facilities in the Northeastern United States using publicly …


Estimation Of Wildlife Damage From Federal Crop Insurance Data, Sophie Mckee, Stephanie A. Shwiff, Aaron M. Anderson Aug 2020

Estimation Of Wildlife Damage From Federal Crop Insurance Data, Sophie Mckee, Stephanie A. Shwiff, Aaron M. Anderson

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Wildlife damage to crops is a persistent and costly problem for many farmers in the USA. Most existing estimates of crop damage have relied on direct assessment methods such as field studies conducted by trained biologists or surveys distributed to farmers. In this paper, we describe a new method of estimating wildlife damage that exploits federal crop insurance data. We focused our study on four crops: corn, soybean, wheat, and cotton, chosen because of their economic importance and their vulnerability to wildlife damage.

RESULTS: We determined crop-raiding hot spots across the USA over the 2015–2019 period and identified the …


Metagenomic, Viral And Host Genetic Analyses Of Congenital Tremor In Pigs, Kylee Marie Sutton Aug 2020

Metagenomic, Viral And Host Genetic Analyses Of Congenital Tremor In Pigs, Kylee Marie Sutton

School of Biological Sciences: Dissertations, Theses, and Student Research

The Pestivirus genus contains several viral species having a major impact on the livestock species. While there were only four major pestiviral species for a time, recent metagenomic sequencing approaches identified additional species, such as atypical porcine pestivirus (APPV). Congenital tremor was first identified almost a hundred years ago and since still has an impact on swine health. There are two main types of congenital tremor, type A and B, with type A congenital tremor further subcategorized based on causative agent. Until recently, type A-II congenital tremor did not have a known cause. Recent viral sequencing of affected samples, including …


New World Mammarenaviral Hemorrhagic Fever Virus Determinants Of Infection And Disease, Brady T. Hickerson Aug 2020

New World Mammarenaviral Hemorrhagic Fever Virus Determinants Of Infection And Disease, Brady T. Hickerson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several New World mammarenaviruses (NWM) are able to cause life-threatening hemorrhagic disease in humans. These agents use human transferrin receptor 1 (hTfR1), the main protein involved in cellular uptake of iron, to infect cells. Furthermore, use of hTfR1 correlates with the ability of these viruses to cause severe disease in humans. The most prevalent pathogenic NWM is Jun√≠n virus (JUNV), which is the etiological agent of Argentine hemorrhagic fever (AHF).The pathogenesis of AHF is poorly understood. A cardinal feature of severe cases of AHF is a prolonged increase of serum interferon-a (IFN-a) concentration. This increase is hypothesized to play a …


Evaluation Of Enrichment And Identification Media For Isolation Of Common Bovine Salmonella Serotypes, Katie Irene Swanson Aug 2020

Evaluation Of Enrichment And Identification Media For Isolation Of Common Bovine Salmonella Serotypes, Katie Irene Swanson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Salmonellosis is an important cause of diarrhea in humans and animals, which can lead to death in severe cases. Rapid and efficient detection of Salmonella spp. is essential to limit the spread of disease. Many enrichment procedures have been described for the isolation of Salmonella, and often include variations on Rappaport-Vassiliadis medium, which is recommended by the Food and Drug Administration (FDA). The current procedures vary in incubation temperature, incubation time, and type of media used for identification of Salmonella. This project compared four procedures for enrichment and identification of common bovine Salmonella serotypes. We determined the best …


Effects Of Freshwater Crayfish On Influenza A Virus Persistence In Water, J. Jeffrey Root, Jeremy W. Ellis, Susan A. Shriner Jul 2020

Effects Of Freshwater Crayfish On Influenza A Virus Persistence In Water, J. Jeffrey Root, Jeremy W. Ellis, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

Several investigations have recently assessed the ability of some aquatic invertebrates to act as tools for avian influenza A virus (IAV) surveillance as well as their potential role(s) in IAV ecology. Because of this, as well as the high IAV seroprevalence rates noted in select mesocarnivores that commonly inhabit aquatic and semi-aquatic habitats, we evaluated the effects that freshwater crayfish have on IAV in water at three dose levels and monitored for the presence of IAV in crayfish tissues (gill and green gland) and haemolymph at multiple time points. At relatively high, medium 432 and low (approximately 10 , 10 …


Brain Complexity, Sentience And Welfare, Donald M. Broom Jul 2020

Brain Complexity, Sentience And Welfare, Donald M. Broom

Animal Sentience

Neither sentience nor moral standing is confined to animals with large or human-like brains. Invertebrates deserve moral consideration. Definition of terms clarifies the relationship between sentience and welfare. All animals have welfare but humans give more protection to sentient animals. Humans should be less human-centred.


Spatiotemporal Genetic Diversity Of Lions Reveals The Influence Of Habitat Fragmentation Across Africa, Caitlin J. Curry, Brian W. Davis, Laura D. Bertola, Paula A. White, William J. Murphy, James N. Derr Jul 2020

Spatiotemporal Genetic Diversity Of Lions Reveals The Influence Of Habitat Fragmentation Across Africa, Caitlin J. Curry, Brian W. Davis, Laura D. Bertola, Paula A. White, William J. Murphy, James N. Derr

Publications and Research

Direct comparisons between historical and contemporary populations allow for detecting changes in genetic diversity through time and assessment of the impact of habitat fragmentation. Here, we determined the genetic architecture of both historical and modern lions to document changes in genetic diversity over the last century. We surveyed microsatellite and mitochondrial genome variation from 143 high-quality museum specimens of known provenance, allowing us to directly compare this information with data from several recently published nuclear and mitochondrial studies. Our results provide evidence for male-mediated gene flow and recent isolation of local subpopulations, likely due to habitat fragmentation. Nuclear markers showed …


An Applied Ecology Of Fear Framework: Linking Theory To Conservation Practice, Kaitlyn M. Gaynor, Michael J. Cherry, Sophie L. Gilbert, Michael T. Kohl, Courtney L. Larson, Thomas M. Newsome, Laura R. Prugh, Justin P. Suraci, Julie K. Young, Justine A. Smith Jul 2020

An Applied Ecology Of Fear Framework: Linking Theory To Conservation Practice, Kaitlyn M. Gaynor, Michael J. Cherry, Sophie L. Gilbert, Michael T. Kohl, Courtney L. Larson, Thomas M. Newsome, Laura R. Prugh, Justin P. Suraci, Julie K. Young, Justine A. Smith

United States Department of Agriculture Wildlife Services: Staff Publications

Research on the ecology of fear has highlighted the importance of perceived risk from predators and humans in shaping animal behavior and physiology, with potential demographic and ecosystem-wide consequences. Despite recent conceptual advances and potential management implications of the ecology of fear, theory and conservation practices have rarely been linked. Many challenges in animal conservation may be alleviated by actively harnessing or compensating for risk perception and risk avoidance behavior in wild animal populations. Integration of the ecology of fear into conservation and management practice can contribute to the recovery of threatened populations, human–wildlife conflict mitigation, invasive species management, maintenance …


Edwardsiella Ictaluri Is Capable Of Persisting In Channel Catfish By Evading Host T Cell And Cell Death Responses, Elizabeth Watts Griggs Jul 2020

Edwardsiella Ictaluri Is Capable Of Persisting In Channel Catfish By Evading Host T Cell And Cell Death Responses, Elizabeth Watts Griggs

LSU Doctoral Dissertations

Edwardsiella ictaluri is a gram-negative bacterium of the family Enterobacteriaceae that infects and causes enteric septicemia (ESC) of channel catfish (Ictalurus punctatus), a fatal disease costing the catfish industry millions of dollars in losses each year. Edwardsiella. ictaluri is capable of replicating in catfish head-kidney-derived-macrophages (HKDM), and like many other gram-negative bacteria, E. ictaluri encodes a Type III Secretion System (T3SS) that is required for virulence and intracellular replication. In the case of E. ictaluri, the T3SS translocates effectors from the Edwardsiella containing vacuole (ECV) through the bacterial cell wall and the vacuolar membrane directly to the …


Growing South Dakota (Summer 2020), College Of Agriculture, Food And Environmental Sciences Jul 2020

Growing South Dakota (Summer 2020), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 COVID-19: From Our Students' Perspective
[Page] 11 Class of 2020 Q&A
[Page] 19 Alumni Advice for the Future Generation
[Page] 21 Recent Alumni Features
[Page] 25 Student Features
[Page] 31 CAFES News, Student Success, Awards and Achievements
[Page] 57 Jackrabbits Now and Then: A Current Student and Alumni Q&A