Veterinary Medicine Commons^™

Influence Of Electromagnetic Stimulation On Blood Macro And Micro Elements In Dairy Cows, Ljiljana Andjusic, Zoran Milankov, Dusan Maric, Bozidar Milosevic, Radojica Djokovic, Marko Cincovic, Nebojsa Lalic, Zvonko Spasic

The Thai Journal of Veterinary Medicine

The aim of this study is to determine the influence of EM stimulations on macro and micro element concentrations in the blood, to determine if changes in ion concentrations depends on their basal values and to determine the usefulness of this kind of stimulation in the maintenance of the blood macro and micro element levels in cows. 17 cows were included in the experimental group (affected by a unique EM stimulation protocol of three weeks duration) and 9 cows were included in the control group. Significantly higher (P<0.01) concentrations of Ca, P, Na and Cu and lower (P<0.05) concentrations of Zn were found in the experimental group after EM stimulation. Dynamic changes of macro and micro element concentrations were inversely proportional to their basalvalue before the beginning of the experiment which means that animals with lower basal concentrations had greater changes of value during stimulation. Dynamicchanges of Ca, P, Mg, Fe, K and Cu were more notable in the experimental group that was exposed to EM stimulation compared to the control group. However, dynamic changes of Na and Zn concentrations were identical in the experimental and control groups. Exposure of cows to EM stimulation had a positive effect at Ca and P concentrations and improved calcaemia and phosphatemia in all the exposed animals.

A Study Of The Temporal Dynamics And Human Exposure To The Formosan Ferret-Badger (Melogale Moschata Subaurantiaca) Rabies, 2013 To 2019, Taiwan, Wen-Jane Tu, Mei-Chuan Wang, Guo-Chin Jau, Cheng-Ta Tsai, Chung-Ching Lin, Satoshi Inoue, Prawit Butudom, Cheng-Hung Lai, Chang-Young Fei

The Thai Journal of Veterinary Medicine

The World Health Organization declared Taiwan rabies-free in 1961. On July 17, 2013, Taiwan confirmed the detection of rabies virus in wild Formosan ferret-badgers.This study investigated the epidemiology of Formosan ferret-badger rabies from July 2013 to December 2019 on Taiwan Main Island. There were two objectives of this study: 1) to study thetemporal dynamics of the epidemics during this period; 2) to assess the risk of human exposure to ferret-badger rabies. Results indicated that atotal of 805 rabies-confirmed ferret-badgers and 9 cases of spillover infection in non-reservoir hosts. The temporal dynamics showed only the epidemic in Eastern Taiwan exhibited the …

Assessment Of Antibacterial And Immunostimulating Activity Of Black Cumin (Nigella Sativa) Extract Against Vibriosis In White Shrimp (Litopenaeus Vannamei), Indriyani Nur, Linianti, Waode Munaeni, La Ode Baytul Abidin, Maulidiyah

The Thai Journal of Veterinary Medicine

It is assumed that the active components of Black cumin seed (Nigella sativa) contain beneficial effects on shrimp health as in humans. Therefore, this study aims to explore the antibacterial and immunostimulant potential of black cumin extract for disease control across V. harveyi. Treatments applied include the administration ofethanol extract mixed into shrimp feed at varying dosages (2500, 5000, 7500 ppm), hereinafter referred to as treatments A, B, C, respectively. All shrimps were exposed to pathogen V. harveyi at a density of 106 CFU ml-1after 14 days of testing duration excluding the negative control group. The immune responses of the …

Gel Protein Supplementation Enhanced Serum Igf-1 Concentration In Pre-Weaning Piglets, Morakot Nuntapaitoon, Junpen Suwimonteerabutr, Nutthee Am-In

The Thai Journal of Veterinary Medicine

The piglet pre-weaning mortality is a major problem in swine production. The objective of the present study was to determine the effect of dietary gel protein supplementation on pre-weaning mortality and growth in piglets. A total of 48 Landrace x Yorkshire crossbred sows were included in this study. On farrowing day, the sows were randomly allocated into two experimental groupsby parity: Control (n=20)and Treatment (n=28).The piglets in the Control group sows (n=234) were not orallysupplemented and the piglets in the Treatment group sows (n=316) were orallysupplemented with gel protein (Porcistart®, Virbac. Co., Ltd., Loudeac, France). The piglets in the treatment …

Acute Oral Toxicity Study Of Ethanol Extract Of Oroxylum Indicum Leaf In Mice, Mohd Farhan Hanif Reduan, Fathin Faahimaah Abdul Hamid, Muhammad Luqman Nordin, Rumaizi Shaari, Ruhil Hayati Hamdan, Eric Lim Teik Chung, Tan Li Peng, Intan Noor Aina Kamaruzaman, Noramalina Noralidin

The Thai Journal of Veterinary Medicine

The Oroxylum indicumplant is aherbal plant commonly eaten by the locals in Malaysia while the application of herbal remedies from the plant has been inherited and passed down through generations. However, there is a lack of toxicity profiling of the plant, hence this research aimed toinvestigate acute oral toxicity ofethanol extract of O. indicumin C57BL/6 male mice at different concentrations, to determine the LD50of the plant extract. A total of twenty-five mice were randomly assigned into five experimental groups comprising the control (normal saline), vehicle (5% DMSO), low dose (1000 mg/kg bw), medium dose (2000 mg/kg bw) and high dose …

Glucosamine Contents Of Milk Hydrolysates From Various Mammals, Zenta Takatsu, Hiroshi Matsumoto, Kumiko Hanaki, Zhihe Zhang, Rong Hou, Hairui Wang, Zheng Yan, Ying Yao, Norikatsu Yasuda, Yukiko Shimokawa, Hiroyuki Kurokawa

The Thai Journal of Veterinary Medicine

It was reported in the authors’ previous study that the glucosamine (GlcN) content of Asian elephantmilk (516 mg GlcN/100 g milk) was markedly higher than that of cow, mare and human breast milk. Based on these findings, the GlcN levels of milk from other mammals, especially herbivores, were analyzed using the identical high performance ion exchange liquid chromatography as was employed in the previous study. The following mg GlcN/(100 g milk) values were obtained for milk hydrolysates from various mammals: Jersey cow 12; water buffalo 6; goat 10; hippopotamus 19; dog 24; bear 97; giant panda 72; seal 114; and …

Dynamic Changes Of Trim62 Distribution In Rev Infected Chickens, Jie Yang, Ling Li, Xiaoman Wang, Ziqiang Cheng, Guihua Wang

The Thai Journal of Veterinary Medicine

The tripartite motif containing 62 (TRIM62) is a host antiviral factor. It was demonstrated in our previous study that chicken TRIM62 significantly inhibited the replication of reticuloendotheliosisvirus (REV). However, the effect of viral infection on the distribution of TRIM62 is still unknown. In the present study, we tested and evaluated the tissue distribution of TRIM62 and the REV infection correlativity. We found that the distribution and mRNA expression of TRIM62 were first increased and then decreased upon REV infection. The decrease of TRIM62 was mostly in the spleen. The results suggest REV inhibited the antiviral activity of TRIM62 by decreasing …

Surveillance For Influenza Virus In Nonhuman Primates (Nhps) In Thailand, 2009-2018, Wanvisa Surarith, Natthaphat Ketchim, Somjit Chaiwattanarungruengpaisan, Supakarn Kaewchot, Paisin Lekcharoen, Chalisa Mongkolphan, Metawee Thongdee, Weena Paungpin

The Thai Journal of Veterinary Medicine

The present study conducted a longitudinal serological survey and active surveillance for influenza virus in free-ranging nonhuman primates (NHPs) in Thailand. One hundred and nine serum samples from NHPs were collected between 2009 and 2017, in 4 provinces of Thailand; while 282 oropharyngeal swabs were collected between April to August, 2018, in 8 provinces of Thailand. Our results demonstrated that no antibody to influenza virus was found in NHPseraby using blocking enzyme-linked immunosorbent (ELISA) assay and hemagglutination inhibition (HI) assay. Moreover, no influenza genome could be detected in all new NHP swab samples by using real-time RT-PCR assay. However, monitoring …

Developing A Rabies Prevention Program Based On Rabies Exposure Data In Nebraska, Jill Oatman

Capstone Experience: Master of Public Health

Eighty-nine percent of Nebraska’s towns are considered rural, with populations of less than 3,000 people and with many towns having less than 1,000 residents. Rural areas consist of crop ground, livestock facilities and regions inhabited by wildlife. Nebraska has two large urban cities, Lincoln and Omaha. Animal and human health in these communities can be greatly impacted by infectious diseases that are transmitted between animals and humans, known as zoonotic diseases. One zoonotic disease of great concern is rabies. Zoonotic diseases not only impact the health of animals and humans, but the social well-being of communities and result in increased …

The Surveillance Of Gastrointestinal Parasitic Nematodes Of Northwest Arkansas Dairy Cattle Using Traditional And Genetic Parasitological Identification Procedures, Eva M. Wray

Graduate Theses and Dissertations

Traditional and genetic parasitological identification procedures were compared using naturaland artificial nematode parasite infections in Holstein steer calves. The traditional parasitological procedures measured fecal egg counts, coprocultures with subsequent larval collection and adult nematodes collected at necropsy. The genetic identification procedures measured ITS-2 sequences extracted from different stages of nematode development: raw feces, concentrated nematode eggs, third stage larvae and adults. The primary nematodes observed were Cooperia oncophora, Cooperia punctata and Ostertagia ostertagi. The traditional techniques were not significantly different from one another, while the genetic sequencing showed variation amongst the different procedures. The raw feces sequences showed the most …

Effect Of Feed Additives And Toxic Elements On Swine Growth Performance, Nutrient Digestibility, Immune Function And Reproductive Performance, Richard A. Mudarra Hernández

Graduate Theses and Dissertations

To evaluate the effects of peptide in combination of zinc oxide (Zno) or acidifiers (Exp 1), and gossypol (G) from cottonseed meal (CSM) ( Exp 2&3) on growth performance, complete blood cell counts (Exp 1, 2&3), nutrient digestibility (Exp 1), plasma gossypol (Exp 2&3) and semen quality (Exp 3), weaned pigs (Exp 1), growing gilts (Exp 2) and growing boars (Exp 3) were randomly allotted to dietary treatments. Treatments for Exp 1 during phase 1&2 were: (1) Positive Control (PC), formulated to meet NRC (2012) nutrient requirements; (2) Negative control (NC), fish meal was reduced to achieve -0.13% SID lysine; …

Identification Of Bovine T-Cell Populations Involved In Placental Growth And Development, Kelsy A. Leppo

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Today, the dairy industry is comprised of cows that can produce around 20,000 pounds of milk in a year, yet producers often see only 30% to 50% of animals produce a live calf after each breeding. While many factors may be at play, there is evidence that the immune system plays a large role in regulating pregnancy and in preventing immune mediated abortions. Maternal immune system changes during pregnancy have been shown to occur both locally in the uterus, which is in direct contact with the placenta, and systemically in the blood. Research in humans and mice has shown that …

Whole Genome Sequencing Identified A 16 Kilobase Deletion On Eca13 Associated With Distichiasis In Friesian Horses, E. A. Hisey, H. Hermans, Z. T. Lounsberry, F. Avila, R. A. Grahn, K. E. Knickelbein, S. A. Duward-Akhurst, M. E. Mccue, Theodore S. Kalbfleisch, M. E. Lassaline, W. Back, R. R. Bellone

Veterinary Science Faculty Publications

BACKGROUND: Distichiasis, an ocular disorder in which aberrant cilia (eyelashes) grow from the opening of the Meibomian glands of the eyelid, has been reported in Friesian horses. These misplaced cilia can cause discomfort, chronic keratitis, and corneal ulceration, potentially impacting vision due to corneal fibrosis, or, if secondary infection occurs, may lead to loss of the eye. Friesian horses represent the vast majority of reported cases of equine distichiasis, and as the breed is known to be affected with inherited monogenic disorders, this condition was hypothesized to be a simply inherited Mendelian trait.

RESULTS: A genome wide association study (GWAS) …

Linking Mosquito Surveillance To Dengue Fever Through Bayesian Mechanistic Modeling, Clinton B. Leach, Jennifer A. Hoeting, Kim M. Pepin, Alvaro E. Eiras, Mevin B. Hooten, Colleen T. Webb

United States Department of Agriculture Wildlife Services: Staff Publications

Our ability to effectively prevent the transmission of the dengue virus through targeted control of its vector, Aedes aegypti, depends critically on our understanding of the link between mosquito abundance and human disease risk. Mosquito and clinical surveillance data are widely collected, but linking them requires a modeling framework that accounts for the complex non-linear mechanisms involved in transmission. Most critical are the bottleneck in transmission imposed by mosquito lifespan relative to the virus’ extrinsic incubation period, and the dynamics of human immunity. We developed a differential equation model of dengue transmission and embedded it in a Bayesian hierarchical …

The Effects Of In Utero Exposure To Aerosols Generated By Electronic Nicotine Delivery Systems In Neonatal And Adult Offspring, Kerin M. Cahill

LSU Master's Theses

Recently, electronic nicotine delivery systems (ENDS) have become increasingly popular alternatives to cigarette smoking and are perceived as “safe” substitutes. The effects that these devices have on healthy adult lungs are still not fully understood, much less the effects on vulnerable populations such as expectant mothers and neonates. In an effort to better understand the potentially detrimental outcomes that gestational usage may have on both mothers and offspring, we conducted mouse studies on in utero exposures to both, third generation (“box mod”) ENDS aerosols and fourth generation (“pod mod”) JUUL aerosols. In exposures using the box mod device, in utero …

Spotted Owls And Forest Fire: Comment, Gavin M. Jones, R. J. Gutiérrez, William M. Block, Peter C. Carlson, Emily J. Comfort, Samuel A. Cushman, Raymond J. Davis, Stephanie A. Eyes, Alan B. Franklin, Joseph L. Ganey, Shaula Hedwall, John J. Keane, Rodd Kelsey, Damon B, Lesmeister, Malcolm P. North, Susan L. Roberts, Jeremy T. Rockweit, Jamie S. Sanderlin, Sarah C. Sawyer, Ben Solvesky, Douglas J. Tempel, Ho Yi Wan, A. Leroy Westerling, Gary C. White, M. Zachariah Peery

United States Department of Agriculture Wildlife Services: Staff Publications

Western North American forest ecosystems are experiencing rapid changes in disturbance regimes because of climate change and land use legacies (Littell et al. 2018). In many of these forests, the accumulation of surface and ladder fuels from a century of fire suppression, coupled with a warming and drying climate, has led to increases in the number of large fires (Westerling 2016) and the proportion of areas burning at higher severity (Safford and Stevens 2017, Singleton et al. 2018). While the annual area burned by fire is still below historical levels (Taylor et al. 2016), some forest types in the west …

Loci Associated With Antibody Response In Feral Swine (Sus Scrofa) Infected With Brucella Suis, Courtney F. Pierce, Vienna R. Brown, Steven C. Olsen, Paola Boggiatto, Kerri Pedersen, Ryan S. Miller, Scott E. Speidel, Timothy J. Smyser

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine (Sus scrofa) are a destructive invasive species widespread throughout the United States that disrupt ecosystems, damage crops, and carry pathogens of concern for the health of domestic stock and humans including Brucella suis—the causative organism for swine brucellosis. In domestic swine, brucellosis results in reproductive failure due to abortions and infertility. Contact with infected feral swine poses spillover risks to domestic pigs as well as humans, companion animals, wildlife, and other livestock. Genetic factors influence the outcome of infectious diseases; therefore, genome wide association studies (GWAS) of differential immune responses among feral swine can provide …

A Review Of Avian Influenza A Virus Associations In Synanthropic Birds, Susan A. Shriner, J. Jeffrey Root

United States Department of Agriculture Wildlife Services: Staff Publications

Avian influenza A viruses (IAV) have received significant attention due to the threat they pose to human, livestock, and wildlife health. In this review, we focus on what is known about IAV dynamics in less common avian species that may play a role in trafficking IAVs to poultry operations. Specifically, we focus on synanthropic bird species. Synanthropic species, otherwise known as peridomestic, are species that are ecologically associated with humans and anthropogenically modified landscapes, such as agricultural and urban areas. Aquatic birds such as waterfowl and shorebirds are the species most commonly associated with avian IAVs, and are generally considered …

Comparative Analysis Of Intestinal Helminth Infections In Colic And Non-Colic Control Equine Patients, Heidrun Gehlen, Nadine Wulke, Antonia Ertelt, Martin K. Nielsen, Simone Morelli, Donato Traversa, Roswitha Merle, Douglas Wilson, Georg Von Samson-Himmelstjerna

Maxwell H. Gluck Equine Research Center Faculty Publications

All around the world, intestinal helminths constitute one of the most prevalent life-long occurring infections and re-infections affecting all horse age groups. A range of parasite species among strongyles, ascarids, and tapeworms is known to have the potential to cause colic in horses. However, there is a lack of current scientific evidence on the actual relevance of helminth infection levels in the context of colic in horses kept during prevailing epidemiological conditions. Thus, a prospective case-control study on the occurrence of intestinal helminths in a total of 620 mainly adult equine clinic patients was conducted to investigate the association between …

Field Evaluation Of Recombinant Antigen Elisa In Detecting Zoonotic Schistosome Infection Among Water Buffaloes In Endemic Municipalities In The Philippines, Jose Ma. M. Angeles, Yasuyuki Goto, Masahi Kirinoki, Elena A. Villacorte, Kharleezelle J. Moendeg, Pilarita T. Rivera, Yuichi Chigusa, Shin-Ichiro Kawazu

Biology Faculty Publications

In this study, we investigated the use of recombinant antigens thioredoxin peroxidase-1 (rSjTPx-1) and tandem repeat rSj1TR in evaluating the antibody positivity rates of Schistosoma japonicum infection among water buffaloes from four endemic areas in the Philippines, two municipalities with high endemicity (Calatrava, Negros Occidental and Catarman, Northern Samar) and two municipalities nearing elimination with no cases of human schistosomiasis (Talibon and Trinidad, Bohol). These recombinant antigen ELISA assays were compared with other diagnostic tests including SEA-ELISA, FECT, and fecal-based PCR. Results showed that rSj1TR-ELISA has the highest agreement with PCR in all study areas. Furthermore, significant positivity rates among …

Meta-Analysis Of Cyathostomin Species-Specific Prevalence And Relative Abundance In Domestic Horses From 1975-2020: Emphasis On Geographical Region And Specimen Collection Method, Jennifer L. Bellaw, Martin K. Nielsen

Maxwell H. Gluck Equine Research Center Faculty Publications

BACKGROUND: Cyathostomins infect virtually all horses, and concomitant infections with 10 or more species per horse is standard. Species-specific knowledge is limited, despite potential species bias in development of disease and anthelmintic resistance. This is the first meta-analysis to examine effects of geographical region and cyathostomin collection method on reported composition of cyathostomin communities.

METHODS: Thirty-seven articles published in English in 1975 or later, in which adults of individual species were systematically enumerated, were included. Seven regions; North America, South America, eastern Europe, western Europe, northern Europe, southern Africa, and Oceania, and three cyathostomin collection methods; (i) standard necropsy recovery …

Prevalence Of Taeniid Eggs In The Faeces Of Domesticated And Free-Roaming Dogs In Basrah, Iraq, And The Knowledge Of Dog Owners On Cystic Echinococcosis, Mohanad Faris Abdulhameed, Ian Robertson, Suzan Abd Al-Jabbar Al-Azizz, Ihab Habib

Karbala International Journal of Modern Science

The larval stages (metacestodes) of some taeniids of carnivores, particularly Echinococcus spp., can result in severe disease for humans and livestock. The prevalence of taeniid eggs by direct examination of faecal smears in 335 free-roaming dogs (FRD) and owned domesticated dogs in Basrah Province was 10.1% (95% CI 7.1, 13.9). A structured questionnaire was administered to 86 dog owners to investigate the influence of socio-demographic factors and management and husbandry practices on their knowledge of cystic echinococcosis (CE). The results of a multivariable logistic regression analysis revealed that dog owners who fed offal had less knowledge of CE (OR=0.17, 95% …

Nad Metabolism In Male Reproductive Aging, Mirella L. Meyer-Ficca

Funded Research Records

No abstract provided.

Tools, Techniques And Teamwork: Transferring New Technologies For Wildlife Management, Conservation And Economic Development, John Eisemann, Gail Keirn

United States Department of Agriculture Wildlife Services: Staff Publications

Did you ever wonder who invented radio telemetry? Or breakaway snares? What about the bird repellent methyl anthranilate or the livestock protection collar? These and many other tools used in wildlife damage management resulted from creative thinking by biologists, technicians, researchers and managers working to solve complex wildlife issues. Over the past 90 years, these “aha moments” within the U.S. Department of Agriculture’s Wildlife Services program have ranged from developing novel scare devices, traps and vaccines to exploiting an animal’s visual capabilities to stop unwanted behaviors. Once discovered, these unique ideas may lead to practical, feasible and cost-effective wildlife damage …

Instability Of Glucocorticoid Metabolites In Coyote Scats: Implications For Field Sampling, Erika T. Stevenson, Eric M. Gese, Lorin A. Neuman-Lee, Susannah S. French

United States Department of Agriculture Wildlife Services: Staff Publications

Studying physiologic stress responses can assist in understanding the welfare of animals. One method of measuring the physiologic stress response is evaluating concentrations of glucocorticoid metabolites in feces. Previously, using an adrenocorticotropic hormone challenge, we found fecal glucocorticoid metabolite levels were a reliable indicator of physiologic stress response in coyotes (Canis latrans). We determine whether glucocorticoid metabolite concentrations remain stable when collecting feces over a 2-week period, a timeframe commonly used in scat surveys for wild canids. We collected feces from 6 captive coyotes maintained at the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Predator …

Importation Of Macrocyclic Lactone Resistant Cyathostomins On A Us Thoroughbred Farm, Martin K. Nielsen, M. Banahan, R. M. Kaplan

Maxwell H. Gluck Equine Research Center Faculty Publications

Anthelmintic resistance in equine cyathostomins is both widespread and highly prevalent in the benzimidazole and tetrahydropyrimidine classes; however, reports of resistance to macrocyclic lactone (ML) drugs are sparse and sporadic. This study reports a case of clear ML resistance in a group of Thoroughbred yearlings imported from Ireland to the US in 2019. Fecal egg count reduction (FECR) following ivermectin administered in February 2020 demonstrated 100% reduction in the US bred yearlings, but 93.5%, 70.5%, and 74.5% reduction in three groups of the imported yearlings. The two former groups were then retreated with ivermectin, yielding FECRs of 33.8% and 23.5%, …

Evaluating Red Wolf Scat To Deter Coyote Access To Urban Pastureland, Meghan M. Louis, Samuel M. Tucker, Michael K. Stoskopf, Suzanne Kennedy-Stoskopf

Human–Wildlife Interactions

Depredation of domestic livestock by wildlife is a leading source of human–wildlife conflict, often requiring intervention at the local level. Historically, these interventions have resulted in the use of lethal methods to remove the offending animal. In response to increased public opposition to lethal control methods, wildlife managers have sought to identify effective nonlethal biological options to mitigate wildlife depredations. In 2018, we tested the concept of a biological deterrent using red wolf (Canis rufus) scat that had historically been spread along fence lines to prevent depredation of lambs (Ovis aries) and kid goats (Capra …

Molecular And Biological Epidemiology Of Mosquito Vecors For Canine Heartworm Infection In The Cumberland Gap Region, Charles T. Faulkner, Michelle Norden

Faculty Other Scholarly Content

Canine heartworm (Dirofilaria immitis), is a nematode parasite that infects canines, both wild and domestic and other vertebrate hosts like ferrets, otters, and cats worldwide. The disease, dirofilariasis, can be fatal in advanced stages if untreated and is recognized as a significant concern in veterinary medicine. Canine heartworm has been reported in 49 states of the United States (U.S.) and is considered enzootic. The nematode lifecycle is dependent upon canines infected with adult worms that produce microfilaria, the vermiform embryonic stage, that circulate in the peripheral blood of the host and the mosquito vectors that ingest them taking …

Influence Of Season On Stereological And Histomorphometric Characteristics Of Testes Of Ouled Djellal Rams In Algeria, Yamina Belkhiri, Souheyla Benbia, Amal Djaout

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the influence of season on testicular morphometry, on ram body weight, testosterone concentration and on stereological structure of the testes in Ouled Djellalrams. Twenty seven rams were randomly selected and the live weight and testicular size were recorded. At the beginning of each season (autumn, winter, spring), among the study group, three rams were randomly selected, slaughtered and their left testes were removed and weighed. For microscopic study, tissue samples were excised from the left testes, fixed in formalin solution and embedded in paraffin and, afterwards, quantitative evaluation was performed. Statistical analysis …

The Effects Of Pumpkin Seed Oil On Carbon Tetrachloride Induced Chronic Hepatic Damage In Rats, Ayhan Atasever, Meryem Senturk, Gorkem Ekebas, Duygu Yaman Gram, Meryem Eren

The Thai Journal of Veterinary Medicine

The present study determined the possible protective effect of pumpkin seed oil (PSO) on histopathological changes in liver tissue and serum alanine aminotransferase (ALT) activity, glucose, Non-Esterified Fatty Acids (NEFA) and triglyceride (TG) levels on chronic liver injury induced by carbon tetrachloride (CCl4) in rats. A total of 80 Wistar rats were divided into eight groups of ten rats each: the first group served as the control, group II was given intraperitoneal 0.2 mL/kg CCl4, twice a week for 8 weeks. Groups III-V were treated daily with PSO for 8 weeks (1, 2 and 3 mL/kg respectively). Groups (VI-VIII) were …