Veterinary Medicine Commons^™

Impact Of Biochar Supplementation In Growing Diets On Greenhouse Gas Emissions, Jessica L. Sperber, Braden C. Troyer, Levi Mcphillips, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

A study was conducted to evaluate the impact of feeding biochar growing diets on cattle performance and methane and carbon dioxide emissions. Two treatments were evaluated, a forage- based control diet without biochar and a diet with biochar included at 0.8% of the diet dry matter, replacing ! ne ground corn in the supplement. Pens of cattle were rotated through a two- sided emissions barn (2 pens evaluated simultaneously) to capture CH4 and CO2 production. " ere were no statistical differences in performance or gas emissions for steers fed a biochar supplemented diet compared to control. Numerically, biochar supplemented steers …

Alternative Heifer Development Systems Utilizing Corn Residue And Cover Crops, Hannah F. Speer, Hannah E. Riley, R. A. Cushman, Harvey C. Freetly, M. E. Drewnoski

Nebraska Beef Cattle Reports

Copyright © 2020 The Board of Regents of the University of Nebraska.

Evaluation Of Biochar On Nutrient Loss From Fresh Cattle Manure, Jessica L. Sperber, Tyler Spore, Galen E. Erickson, Andrea K. Watson

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the impact of biochar and time on manure nutrient retention. Pans were used to simulate feedlot pens with 10 replications per treatment. Biochar was included at 0, 5, or 10% of manure dry matter with 30 and 60 day durations to evaluate pan contents over time. There was a 13- percentage unit increase in organic matter losses from day 30 to 60 for pans without biochar, and a 3- percentage unit increase for pans containing biochar. The least nitrogen loss was measured on the pans without biochar harvested at 30 days. Pans harvested at …

Continental-Scale Dynamics Of Avian Influenza In U.S. Waterfowl Are Driven By Demography, Migration, And Temperature, Erin E. Gorsich, Colleen T. Webb, Andrew A. Merton, Jennifer A. Hoeting, Ryan S. Miller, Matthew Farnsworth, Seth R. Swafford, Thomas J. Deliberto, Kerri Pedersen, Alan B. Franklin, Robert G. Mclean, Kenneth R. Wilson, Paul Doherty

United States Department of Agriculture Wildlife Services: Staff Publications

Emerging diseases of wildlife origin are increasingly spilling over into humans and domestic animals. Surveillance and risk assessments for transmission between these populations are informed by a mechanistic understanding of the pathogens in wildlife reservoirs. For avian influenza viruses (AIV), much observational and experimental work in wildlife has been conducted at local scales, yet fully understanding their spread and distribution requires assessing the mechanisms acting at both local, (e.g., intrinsic epidemic dynamics), and continental scales, (e.g., long-distance migration). Here, we combined a large, continental-scale data set on low pathogenic, Type A AIV in the United States with a novel network-based …

Avian Use Of Exotic Street Treescapes In Metropolitan Areas Of Phoenix, Arizona, Brian E. Washburn, Karen Hoss, David L. Bergman

United States Department of Agriculture Wildlife Services: Staff Publications

Urban areas are highly modified environments that are strongly influenced by a variety of anthropogenic factors. Consequently, these areas contain unique wildlife communities typically dominated by species that are generalist in nature or highly adaptable. We examined the use of five species of exotic treescapes by exotic and native birds in metropolitan areas of Phoenix, Arizona. House Sparrows [Passer domesticus (37%)], European Starlings [Sturnus vulagris (27%)], Mourning Doves [Zenaida macroura (11%)], and Great-tailed Grackles [Quiscalus mexicanus (7%)] were the most frequently observed species during the study. Approximately two-thirds (67%) of the birds observed during the study were exotic species. Avian …

Diets Of Double-Crested Cormorants In The Lake Winnebago System, Wisconsin, Ryan P. Koenigs, Daniel J. Dembkowski, Charles D. Lovell, Daniel A. Isermann, Adam D. Nickel

United States Department of Agriculture Wildlife Services: Staff Publications

Double-crested cormorant Phalacrocorox auritus Lesson (cormorant) populations have increased throughout the Great Lakes region of North America causing concern related to the impact of cormorant predation on fish communities. A recent decline in yellow perch Perca flavescens (Mitchill) abundance within the Lake Winnebago System, Wisconsin, USA, prompted an assessment of cormorant diets to evaluate potential effects of cormorant predation on the sportfish community. Diets were collected from 883 cormorants (417 from Lake Winnebago and 466 from Lake Butte des Morts) between 2015 and 2017. Cormorant diets on both waterbodies consisted mostly of freshwater drum Aplodinotus grunniens Rafinesque and gizzard shad …

Evaluation Of A Warfarin Bait For Controlling Invasive Wild Pigs (Sus Scrofa), James C. Beasley, Lindsay M. Clontz, Allison Rakowski, Nathan P. Snow, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Wild pigs (Sus scrofa) cause widespread environmental and economic damage, and as a result are subjected to extensive control. Current management strategies have proven insufficient, and there is growing interest in use of toxicants to control invasive populations of this species. In 2017 a low-dose warfarin bait was federally approved for use in controlling wild pigs in the United States. However, no states have allowed use of this bait due to unanswered questions regarding welfare concerns, field efficacy, and non-target impacts.

RESULTS: All captive wild pigs fed 0.005% warfarin baits in no choice feeding trials succumbed in an average …

Molecular Characterization And Phylogenetic Analysis Of Infectious Bursal Disease Virus (Ibdv) From Commercial Broiler Farms In Luzon, Philippines, Mark Lawrence G. Atienza

Undergraduate Theses

Decades after its discovery, the infectious bursal disease virus (IBDV) still poses significant risks in poultry industry, due to its immunosuppressive effect on afflicted animals. This study aims to determine the different genotypes of IBDV that are present in the country, through molecular characterization and phylogenetic analysis of the VP2 hypervariable domain of infectious bursal disease virus. Nested RT-PCR was used to confirm the presence of IBDV in samples collected from 10 farms. Subsequent genetic analysis through nucleotide sequencing and phylogenetic analysis revealed the presence of three (3) variants in Luzon broiler farms. The variant IBDV was found to be …

Molecular Detection Of Aquatic Bird Bornavirus In Waterfowls In Calamba, Laguna, Gio Tristan J. Esguerra

Undergraduate Theses

Waterfowls are believed to be reservoirs and transmitters of aquatic bird bornavirus. The virus targets the central and peripheral nervous system and causes neurologic abnormalities and/or gastrointestinal dysfunction due to the involvement of the gastrointestinal autonomic nervous system. Infected waterfowls are typically asymptomatic and tend to be lifelong carriers and long-term shedders of the virus. A total 81 fecal samples were collected from five enclosures housing various species of waterfowls from an aviary in Calamba, Laguna. The samples were analyzed by RT-PCR using primers that target a highly conserved region of the matrix (M) gene located between nucleotide positions 1911 …

Development Of Elisa For Detecting Antibody Against Bartonella Spp. Specific Protein In Cats And Its Application In Natural Infection, Krissda Boonaramrueng

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aims to evaluate the risk factor and an association between Bartonella spp. infection and immune status by using retroviral-infected cats as an immunocompromised model and to develop the recombinant B. henselae specific antigen protein (17kDa and GroEL)-based ELISA test for antibody detection against Bartonella spp. infection in cats. From 2017 to 2020, 161 client-owned clinical healthy cats at veterinary clinics and hospitals in the Bangkok metropolitan area were recruited and tested for Bartonella spp. infection statuses (PCR and IFA serology), blood profiles, feline retroviral statuses (FIV and FeLV), and T lymphocyte subsets (CD4+, CD8+, and CD4+ to CD8+ …

Dexamethasone Serum Concentrations After Intravenous Administration In Horses During Race Training, S. Mcclure, C. K. Fenger, Bradley Brown, George Maylin, W. Duer, L. Dirikolu, Kimberly Brewer, Jacob Machin, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Dexamethasone (DXM) sodium phosphate is a widely used corticosteroid for inflammatory conditions in horses, regulated in racing jurisdictions in the USA by a 0.005 ng/ml serum/plasma threshold. This study seeks to describe serum concentrations of DXM at 48 and 72 h after intravenous administration of 20 mg DXM sodium phosphate over 1 to 5 days, and to identify a possible source of DXM overages. 74 horses (39 Thoroughbreds, 13 Standardbreds, 22 Quarter Horses) in active race training received 20 mg DXM sodium phosphate. Serum was collected before injection, at 48 and 72 h post last injection, and analysed by LC/MS-MS …

Detection Of Varroa Mites From Honey Bee Hives By Smart Technology Var-Gor: A Hivemonitoring And Image Processing Device, Sedat Sevi̇n, Hi̇dayet Tutun, Seli̇me Mutlu

Turkish Journal of Veterinary & Animal Sciences

The major honey bee parasite Varroa destructor reduces hive vitality and honey yields by preventing growth of heathy bees and causes drastic loss in apiculture. Therefore, mite infestation in honey bee needs to be constantly controlled by beekeepers. To minimize this loss, a system called Var-Gor has been developed with the aim of controlling Varroa mite infestation before/just after entering the hive instead of the late period. Var-Gor is a hive entrance attachable device box consist of bee passage tunnels (width: 25 mm, height: 15 mm, and depth: 50 mm), autofocus detection camera combined with interface (process sensor: IV-HG10) and …

Effects Of Different Selection Strategies On Subsequent Breeder Performance, Slaughter,And Carcass Traits Of Japanese Quail, Sohail Ahmad, Jibran Hussain, Hafiz Muhammad Ishaq, Athar Mahmud, Shahid Sherzada, Muhammad Usman, Abdur Rehman, Muhammad Zaid

Turkish Journal of Veterinary & Animal Sciences

The present study evaluated the effect of different selection strategies in four closebred flocks (CBF) at three ages on subsequent breeder performance and carcass traits. A total of 540 Japanese quails already selected for higher body weight from four CBF (Major, Kaleem, Saadat, and Zahid) at three ages (10, 12, and 14 weeks) were subjected to 3 selection strategies (i.e. pedigree, mass selection, and randombred control). In pedigree selection, 108 birds with higher body weight were selected with full pedigree record whereas, in the mass selection, 324 birds with higher body weight were selected to be the parents of the …

Effect Of Egg Weight On Development, Bone Morphology, And Breast Muscle Histology Of Embryos From Fast-And Slow-Growing Strains, Mojtaba Vafaeinia, Gamze Turgay İzzetoğlu, Zi̇şan Servet Yalçin

Turkish Journal of Veterinary & Animal Sciences

The present study aimed to determine the impact of egg weight on embryonic development, breast muscle histology, and bone morphology of fast- and slow-growing embryos and chicks. For this purpose, total 192 eggs from fast-(Ross 308) (FG) and slow-growing (Hubbard JA) (SG) broiler breeders were used. Eggs were classified as medium (64 ± 1 g, Megg) and heavy (72 ± 1 g, Hegg) eggs. At 18 days of incubation, egg weight loss, embryo weight, embryo length, breast width and length, yolk sac weight, and bone morphometric parameters were measured and breast muscle histology were studied. The same measurements were repeated …

Investigating The Effect Of Decontaminants On Microbiological And Chemical Properties Of Rainbow Trouts, Abdullah Di̇ki̇ci̇, Emi̇ne Özpolat, Sümeyye Betül Bozatli, Ahmet Koluman, Bahri̇ Patir, Mehmet Çalicioğlu

Turkish Journal of Veterinary & Animal Sciences

This study was designed to determine the effect of decontamination on the shelf life of whole rainbow trouts. For this purpose 0.5% cetylpyridinium chloride (CPC), 10% trisodium phosphate (TSP), 2.5% acetic acid (AA), 2.5% lactic acid (LA), 1200 ppm acidified sodium chloride (ASC) and control (tap water) were used as decontaminants. After the decontamination process, the samples were stored in cold storage and subjected to microbiological and chemical analyzes on days 0, 3, 6, 9, 12 and 15. Mesophilic bacteria, psychrophilic bacteria, lactic acid bacteria, Pseudomonas spp., Enterobacteriaceae and coliform bacteria were enumerated for the evaluation of microbiological quality, whereas …

The Effect Of The Metabolic Syndrome On The Histological Structure Of The Testes Tissue And The Sperm Morphology In The Rats, Ayşe Alkandurur, Şadi̇ye Kum

Turkish Journal of Veterinary & Animal Sciences

In this study, we aimed to investigate the effect of metabolic syndrome on spermatozoon morphology and histological, histochemical, histomorphometric changes in the testes. Twenty-four male Sprague Dawley rats (60 day-olds) were used for this purpose. The rats in the control group were fed only water and rat food, while the rats in the experimental group were fed with water containing 20% D-fructose and rats food for 16 weeks with ad libitum. At the end of the experimental period, the testes and epididymis of the rats were removed. The testes were embedded in paraffin by performing the necessary histological procedure. In …

Effect Of Buck Existence On Some Reproductive Hormone Levels During Premating Inakkeçi Goats, Ali̇ Şenok, Erkan Pehli̇van, Fati̇n Cedden

Turkish Journal of Veterinary & Animal Sciences

In this study, the effect of buck presence on serum progesterone, estrogen and FSH levels during the premating in 20 female Akkeçi goats was investigated. The goats were assigned to two groups as follows: control group (n = 10) and treatment group (n = 10). At the beginning of study, first blood samples (day 0) were taken from each goat kept isolated away from bucks at mating season. At day 13, female goats in treatment group were placed in a pen next to, that of bucks in order to provide buck effect. Second blood samples were taken from each does …

Efficacies Of Thai Probiotic Strains Against Antibiotic Resistance Bacteria And Modulate Gut Microbiome And Resistome In Pig Model, Prasert Apiwatsiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lactic acid bacteria (LAB) have been widely used as probiotics in the livestock industry because of their high potential for antibacterial activity, anticonjugation, antibiofilm capacity, and gut microbiome regulation. Lactobacillus plantarum 22F, 25F (L22F and L25F), and Pediococcus acidilactici 72N (P72N) showed several promising in vitro and in vivo properties, according to our recent research. The objective of this study was to evaluate the Thai LAB efficacy on anticonjugation and antibiofilm activities in E. coli harboring the mcr-1 gene. When compared to the neutralizing cell-free supernatant (CFS), we discovered that the CFS derived from our LAB strains at 1:16 dilution …

Molecular Detection And Genetic Diversity Of Rotavirus A In Domestic Dogs And Cats In Bangkok, Thailand, Ekkapat Chamsai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Rotavirus A (RVA) in one of the important pathogens causing gastroenteritis in humans and many animal species. The zoonotic potential of RVA has been reported and raises major concerns, especially in high animal-human interfaces settings. The objectives of this thesis were to determine the occurrence of RVAs in dogs and cats, and to investigate the genetic diversity and genetic relationship among RVAs in dogs, cats, human, and other animal species. The total of 572 rectal swab samples were collected from dogs and cats in animal hospitals, in Bangkok and vicinities, during January 2020 to June 2021. The conventional one-step RT-PCR …

In Vitro Generation Of Transplantable Insulin-Producing Cells From Canine Adipose-Derived Mesenchymal Stem Cells, Quynh Le Dang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine mesenchymal stem cells (cMSCs) have potential applications for regenerative therapy, including the generation of insulin-producing cells (IPCs) for studying and treating diabetes. In this study, we established a useful protocol for generating IPCs from canine adipose mesenchymal stem cells (cAD-MSCs). Subsequently, in vitro preservation of pluronic F127-coated alginate (ALGPA)-encapsulated cAD-MSC-derived IPCs was performed to verify ready-to-use (RTU) IPCs. IPCs were induced from cAD-MSCs with the modulated three-stepwise protocol. The first step of definitive endoderm (DE) induction showed that the cooperation of Chir99021 and Activin A created the effective production of Sox17-expressed DE cells. The second step for pancreatic endocrine …

Active And Passive Immune Responses And Protection Of Nile Tilapia (Oreochromis Niloticus) Immunized With Tilapia Lake Virus Inactivated Vaccines, Thao Thu Mai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tilapia tilapinevirus, also known as tilapia lake virus (TiLV), is a contagious viral pathogen, resulting in mass mortalities and economic losses for tilapia industry. Here, we developed two simple cell-culture, heat-killed and formalin-killed vaccines (HKV and FKV) aiming to prevent this disease. The vaccine efficacies were evaluated by intraperitoneal injection in juvenile tilapia with each vaccine containing 1.8 × 106 TCID50 inactivated virus, followed by a booster dose at 21-day post primary vaccination (dppv) in the same manner. At 28 dppv, the fish were challenged with a lethal dose of TiLV. Expression of five immune genes in head kidney and …

Dirofilaria Immitis Prevalence In Canis Latrans In Kentucky, Melanie Ann Brandon

Murray State Theses and Dissertations

Dirofilaria immitis (canine heartworm) was found in forty-two (42) of the two hundred seventy-five (275) Canis latrans (coyote) necropsied in the state of Kentucky from November 27, 2019 through March 3, 2021. Thirty-five (35) of the positive cases were from western Kentucky region with the other seven spread across the state. With this group of coyotes, one hundred fifty-eight (158) were male and the other one hundred six-teen (116) were female. The estimated age ranged from a pup to senior dogs. A little over forty percent of the dogs were obtained through coyote/predator tournaments; the remaining were acquired from pest …

Effects Of Dietary Supplementation Of Citric Acid And Mannanase On The Growth Performance Of Broilers, Maria Patricia T. Arellano

Undergraduate Theses

The study was conducted to evaluate the effects of citric acid and mannanase feed supplementation on the growth performance of broiler chickens. Three hundred twenty (320) day-old unsexed Cobb broiler chicks from a commercial hatchery plant were used in the study. The chicks were randomly assigned to four treatment groups following a completely randomized design. The following treatment groups were used: T1: Control, T2: 3% Citric Acid supplementation, T3: 80 ppm Beta-Mannanase supplementation, and T4: 3% Citric Acid and 80 ppm Beta-Mannanase supplementation. The production parameters for growth performance that were evaluated are body weight, body weight gain, feed intake, …

Prevalence Of Canine Atopic Dermatitis And Distribution Of Causative Allergens In Bangkok, Sopon Sornsanit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine atopic dermatitis (CAD) is a pruritic allergic skin disease caused by an overreaction to environmental allergens. The diagnosis of CAD is made on the basis of the patient's history, physical examination, and exclusion of other pruritic skin diseases. Allergen-specific IgE serology (ASIS) is one of the most frequently recommended tests in veterinary dermatology for identifying causative allergens. The purpose of this study was to determine the prevalence of CAD and the types of allergens that cause it in atopic dogs using ASIS in the Bangkok metropolitan area. The study was divided into two sections. Between 2015 and 2019, 383 …

Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species, Karim Abdelsalam

Electronic Theses and Dissertations

BVDV is a major pathogen of ruminants that causes immune dysfunction and lead to secondary bacterial infection. The underlying mechanism of this dysfunction is not well understood. Based on previous results, we believe that macrophages play a central role in BVDV-associated immune dysfunction. Previously, we have shown that the infected monocyte-derived macrophage (MDM) supernatant of the high virulent strains of BVDV significantly induced lymphocyte apoptosis. In the current study, we performed experiments to identify the apoptotic factors in the infected MDM supernatants in vitro. Also, we have extended our investigation to include testing the impact of infected macrophages on neutrophil …

Genetic Variation In The Bovine Myogenic Determination Factor 1 (G.782g>Apolymorphism) And Its Influence On Carcass Traits In Turkish Grey Steppe Cattle, Sena Ardiçli, Özden Çobanoğlu

Turkish Journal of Veterinary & Animal Sciences

MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic …

Effect Of Neem (Azadirachta Indica) And Tulsi (Ocimum Sanctum) Leaves Powder Ongrowth Performance, Hemato-Biochemical Profile And Carcass Traits Of Japanese Quail (Coturnix Japonica), Luqman Arshad, Syed Zeeshan Haider Naqvi, Jibran Hussain, Hala Naeem, Saima Shahzad, Amna Aslam, Abd Ur Rehman, Abdul Ghayas

Turkish Journal of Veterinary & Animal Sciences

The current research investigated the growth performance, hematology, serum biochemistry of Japanese quails fed Neem (Azadirachta indica) and Tulsi (Ocimum sanctum) leaves powder with basic diet. A total number of 300 one-week old Japanese quail chicks were divided randomly into four dietary treatments with five replicates each (15 birds per replicate). Dietary treatments were: T0 or control or basal diet, T1 (Neem 0.5%), T2 (Tulsi 1%) and T3 (Neem 0.25% + Tulsi 0.5%) in basal diet. Growth performance parameters (feed intake, body weight, weight gain and feed conversion rate) were determined weekly. Hematology, serum biochemistry, carcass traits were evaluated on …

Elastography Acoustic Radiation Force Impulse (Arfi): An Investigation Of Ocularelasticity In Dogs With Chronic Secondary Glaucoma, Gabriela Morais Madruga, Marcus Antonio Rossi Feliciano, Marjury Cristina Maronezi, Marcella Rosa Filézio, Thaís Guimarães Morato Abreu, Alexandre Pinto Ribeiro, Feancimery Aparecida Fachini, Karina Kamachi Kobashigawa, Igor Cezar Kniphoff Da Cruz, Ricardo Andres Ramirez Uscategui, José Luiz Laus

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate and compare the elasticity of the optic nerve (ON) and retinal-choroid-sclera complex (RCSC) in dogs with healthy eyes and secondary glaucoma using ARFI elastography. ARFI elastography was performed on normal eyes (n = 25) and affected by glaucoma (n = 25). The mean values of shear wave velocity (SWV) of the ON and RCSC were compared between groups by the Student?s t-test. The correlation between elastography measurements of the ON and RCSC and the intraocular pressure (IOP) was measured. The SWV of the optic nerve in glaucomatous eyes (4.77 ± 1.59 m/s) …

Antioxidant Supplementation Ameliorates Bull Sperm Parameters And Fertilizing Abilityfollowing The Freeze-Thaw Process, Pürhan Barbaros Tuncer, Serpi̇l Sariözkan, Mustafa Numan Bucak, Serhat Büyükleblebi̇ci̇

Turkish Journal of Veterinary & Animal Sciences

This study's goal was to reveal the effects of antioxidant supplementation on motility, motion characters, morphology, DNA integrity, and fertilizing potential of cryopreserved bovine sperm. The ejaculates were collected from three Holstein bulls. At least ten ejaculates were collected per bull. The ejaculates were immediately separated into five aliquots, diluted in the Cryobos (Magapor Co. Ltd., Zaragoza, Spain) commercial extender, including 2.5 mM taurine, 2.5 mM cysteine, 2.5 mM methionine, 2.5 mM glutamine, and no additives were used. Afterward, they were frozen in 0.25 mL French straws, and liquid nitrogen was used for the storage of semen. The supplementation of …

Identification Of Micrornas In The Follicular And Luteal Tissues Of Holstein-Friesian Cows, Selçuk Özdemi̇r, Seli̇m Çomakli

Turkish Journal of Veterinary & Animal Sciences

MicroRNAs (miRNAs) regulate many physiological pathways, including development, cell differentiation, immune response as well as diseases by post-transcriptional processes. However, information is limited regarding the biological roles of miRNAs in the development of the follicular and luteal tissues in cows. The main aim of the present study was to identify miRNAs that are expressed in the follicular and luteal tissues of cows. We used a comparative RNA sequencing method to identify miRNA candidates that might play key roles in the follicular and luteal phases of Holstein cows. Nine miRNAs were expressed at a high ratio: 8 in the follicular tissue …