Veterinary Medicine Commons^™

Supplementation Of Extracellular Adenosine 5'-Triphosphate Improves Sperm Post-Thawed Quality In Thai Native Chickens, Phakjira Sanguansook, Junpen Suwimonteerabutr, Kaywalee Chatdarong

The Thai Journal of Veterinary Medicine

Thai native chicken meat is a high-quality protein source of unique flavor. Genetic preservation and breeding programs are beneficial to maintain purebred populations. Aiming at improving post-thawed sperm quality, this study is the first to apply extracellular adenosine 5'-triphosphate (ATPe) in freezing extender to preserve Thai native chicken sperm. Eight semen samples from eight Thai native chickens (n = 8) were included. Initial semen evaluation and sperm size measurement were performed. Beltsville poultry semen extender supplemented with 0 and 30 mM ATPe was served as a control and treatment group, respectively. After thawing, motility and motion characteristics were evaluated by …

Diagnostic Imaging And Hematological And Cytological Analyses Play A Vital Role In Clinical Investigation And Selection Of Therapeutic Approaches In A Polo Pony With Septic Olecranon Bursitis, Rungrueang Yodsheewan, Ravisara Limsripan, Metha Chanda

The Thai Journal of Veterinary Medicine

A 19-year-old gelding polo pony was presented with a prominent protuberance at the tip of the right elbow and five days later, developed a marked swelling on the entire right foreleg with a high fever. Ultrasonography, performed on the caudal elbow region, demonstrated heterogeneous echogenic materials encapsulated in the fibrous capsule. The initial hematological analysis revealed anemia and a normal white blood count (WBC). Hyperbilirubinemia was detected biochemically. Pale yellow/turbid fluid was aspirated from the protuberance area, which showed septic exudate characteristics and contained Streptococcus spp. The pony was diagnosed with septic olecranon bursitis associated with streptococcal infection. The fluid-filled …

Stifle Joint Reconstruction Technique Using Fascia Lata In A Cat With Cranial Cruciate Ligament Rupture, Hern-Yeu Lee, Wei-Yau Shia, Tien-Huan Hsu, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

A two-year-old spayed female mongrel cat presenting with right hind limb lameness with intermittent weight bearing while walking was taken to the veterinary medicine teaching hospital of National Chung Hsing University. Both drawer test and tibial compression test were positive, stifle radiography showed synovial fluid had become more radiopaque. The tentative diagnosis for the cat was rupture of the right cranial cruciate ligament (CrCL), medical management using corticosteroids was prescribed with anticoagulants and surgical treatment recommended. Intracapsular reconstruction was performed and the postoperative recovery of this cat presented normal physical status with improved right hind limb weight-bearing activity. This report …

Mycotic Pyogranulomatous Enteritis In A German Shepherd: Imaging Diagnosis And Treatment, Phasamon Saisawart, Somchin Sutthigran, Apichart Srifawattana, Onjira Mitroupathum, Tanhatai Krajarngjang, Anudep Rungsipipat, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

A five-year old, intact male, German Shepherd dog was presented to the hospital due to a history of depression, anorexia, vomiting and diarrhea. Physical examination revealed moderate emaciation and showed mild abdominal pain when Palpation. Abdominal radiographs and ultrasounds showed the distension of the small bowel loop due to partial obstruction of the colon by mural concentric thickening. Computed tomography (CT) scan showed an homogeneous, soft tissue mass with asymmetric thickening of the cecum traversing the colonic wall. Pre-operative blood profiles indicated normocytic normochromic mild anemia, hypoalbuminemia and mild hyperglobulinemia. Intestinal resection and anastomosis at the distal ileum to the …

Gangliosidoses In Cats, Latticha Pluemhathaikij, Waruntip Bunyaputikul, Katriya Chankow, Benchaphorn Limcharoen, Nardtiwa Chaivoravitsakul, Rampaipat Penchome, Sawang Kesdangsakonwut, Kasem Rattanapinyopituk, Wijit Banlunara

The Thai Journal of Veterinary Medicine

Two 3-month-old, male, domestic short hair, cats of the same litter were presented to the small animal teaching hospital with the clinical sign of head tremor for 2 weeks. Three months later, the clinical sign had worsened. Both kittens had loss of balance and showed intention tremor. Physical examination revealed hypertelorism, depressed bridge of the nose and back pain. Radiography showed shortened cervicothoracic vertebral bodies. Both kittens died at 8 and 9 months of age, case no.1 and 2 respectively. Both carcasses were submitted for necropsy. Both brains were grossly normal. The costal cartilages of both cats were deformed into …

Hepatotoxicity In Acute And Subchronic Oral Gavage Of Ethanolic Extract Of Christia Vespertilionis (L.F.) Bakh. F. Leaf In Male Sprague Dawley Rats, Nurul S.A.S., Nurul S.A.S., Hazilawati H., Rosly S.M., Farhan Hanif M.R., Abdullahi A., Shanmugavelu S., Noordin M.M., Mazlina M., Azrina A., Norhaizan M.E.

The Thai Journal of Veterinary Medicine

Increased interest in Christia vespertilionis (L.f.) Bakh. f. leaves is due to their real potential of their health-enhancing properties. This study aims to evaluate the possible in vivo toxicity in 14-day acute and 90-day subchronic oral toxicity of ethanolic extract Christia vespertilionis (L.f.) Bakh. f. in male Sprague Dawley rats. In the acute study, the rats were divided into control, 5% DMSO (vehicle) and 2000 mg/kg groups. For the subchronic toxicity study, a total of 30 rats were divided into five groups consisting of a control, 5% DMSO (vehicle), low dose (75 mg/kg), medium dose (125 mg/kg) and high dose …

Recombinant Scfv Inhibit Newcastle Disease Virus In Vitro Infection, Sanfeng Peng, Lintian Yu, Ying He, Dongyang Chen, Li Wang, Xueliang Zhou, Long Xie, Yangqing Lu

The Thai Journal of Veterinary Medicine

Newcastle disease (ND) is an avian respiratory disease caused by the Newcastle disease virus (NDV) and is prevalent worldwide but lacks a reliable treatment to surmount it. The single-chain fragment variable (scFv) antibody is the smallest functional unit in an immunoglobulin retaining antigen-binding activities. Previous studies have demonstrated that by acting against the NDV phosphoprotein, scFv is capable of neutralizing the virus in infected cells. In this study, two anti-NDV scFv expressing cassettes driven by CAG promoter and EF1α promoter, respectively, were constructed using the piggyBac transposon plasmid backbone. The recombinant plasmids were transfected into DF-1 and 293T cells and …

Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate, Bo Dong, Bo Dong, Xiaodong Zhang, Xiaodong Zhang, Yafei Song, Chengyu Li, Gaoqiang Zhang, Weiming Lin

The Thai Journal of Veterinary Medicine

FCoV viruses exhibit great genetic diversity, leading to the presence of FIPV-causing variants. Current molecular evolution analysis and genetic variation studies of FCoV in China are predominately focused on gene encoding the spike protein or other structural proteins, while few studies have evaluated genetic variations in nonstructural FCoV genes, which can play an important role in disease pathogenesis. In this study, the gene encoding the open reading frame (ORF) 7b nonstructural FCoV protein of the Chinese Fujian strain FJLY20201 was amplified from the ascitic fluid of a Chinese domestic cat infected with FIPV and compared with ORF 7b from previously …

Yield, Composition, Fatty Acid Profile And Cla Content Of Milk From Goats Fed With Different Levels Of Opf, Pitunart Noosen, Ulia Renfelia Baysi

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the effect of different inclusion levels of OPF in total mixed ration (TMR)-based diet on yield, composition, FA profile and CLA content of milk in dairy goats. Six nulliparous female dairy goats (Saanen x Thai native, 19-21 months old, first lactation) were randomly set according to a 3x3 Latin square design with 2 replications for receiving diets consisted of 0% OPF (OPF0), 20% OPF (OPF20) and 40% OPF (OPF40). The average weight of the goats was 32.48±1.30 kg (mean±s.d.). The findings of this study showed that dry matter intake (DMI) and all …

Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam, Tran Duc Hoan, Doan Thi Thao, Nguyen Thi Huong Giang, Nguyen Thi Thu Huyen, Nguyen Thi Thu Huyen

The Thai Journal of Veterinary Medicine

To identify further the infectivity, genetic diversity and molecular characteristics of the porcine epidemic diarrhea virus (PEDV). 286 samples of the intestinal tissue of pigs with severe diarrhea were assessed from 5 provinces in the north of Vietnam. The full-length ORF3 gene of 20 representative field strains from 36 farms in 5 provinces was sequenced and analyzed. The prevalence of PEDV was 90.56% and was detected in 259 of 286 samples, with 94.44% (34 of 36) of pig farms harboring the disease. Based on the phylogenetic analysis of the ORF3 genes, our isolates all fell into group G2 (variants) and …

Investigation Of The Microstructure And Mechanical Properties Of The Domestic Cat’S Paw Pads, Ye Mei

The Thai Journal of Veterinary Medicine

Domestic cats have a biological function such as cushioning when they are jumping or walking which is mainly due to the biological coupling system formed in the long-term evolution process of nature, including the trunk-leg-paw pad structure. Among them, the paw pad, as the only part of the body that directly contacts with the ground in the process of movement, plays a key role in its biological function. Hence, in order to clarify the micro structure, mechanical properties and functional characteristics of the paw pad and reveal the relationship between it and the domestic cat’s biological function, this paper has …

Optimal Infusion Rate Of Hypertonic Saline To Encephalic Vessel Vasodilatation Using Magnetic Resonance Imaging In Normovolemic Dogs, Yoshiki Murakami, Kenji Tsukano, Haruyuki Hirata, Hiroshi Koie, Kazuyuki Suzuki

The Thai Journal of Veterinary Medicine

This study aimed to investigate, using magnetic resonance imaging, whether continuous hypertonic saline (HSS) infusion induces a superior vasodilating effect on the encephalic vessels compared to bolus infusion in dogs. The relative cross-sectional area of superior sagittal sinus increased to 1.96±0.09 and 1.69±0.31 times of the pre-value in the continuous and bolus groups, respectively, at t=30 min (p<0.001). However, the relative cross-sectional area of superior sagittal sinus was larger in the continuous group than that in the bolus group (p<0.001). The results suggest that continuous infusion of HSS might be superior to bolus infusion in inducing vasodilatation of the encephalic circulation.

Oxygenated Dressing Quickens Skin Wound Healing Of Bama Miniature Pigs, Yingqiu Wang, Limin Qiao, Hong An, Zhenhao Zhang, Yan Zheng, Chang Chen, Jingrui Zhang, Xiangguo Wang

The Thai Journal of Veterinary Medicine

Oxygen is a vital factor in the process of wound healing and is involved in collagen synthesis, synthesis and the release of growth factors, angiogenesis and epidermal cell contraction. Hence, local oxygen control of wounds plays an important role in skin wound healing. Herein, we developed a novel oxygenated dressing for the treatment of skin wound healing in Bama miniature pigs. The oxygenated dressing was mainly composed of potassium iodide, glucose, glucose oxidase, polyvinyl alcohol, polypropylene, polyvinylpyrrolidone and water. The oxygenated dressing significantly quickens the healing of full-thickness skin wounds in Bama miniature pigs and promotes the proliferation of epidermal …

Feline Inductive Odontogenic Tumor In A Young Cat, Chakkarin Satthathum, Sunee Kunakornsawat, Chaiyan Kasorndorkbua

The Thai Journal of Veterinary Medicine

A 6-month-old, 4.45 kg, intact, male domestic shorthair cat was presented with a gingival mass located on the left rostral maxilla and second left upper premolar tooth. Intraoral x-ray of the left rostral maxilla revealed left maxilla bone lysis with a unilocular cystic lesion. The cytology result revealed an epithelial cell tumor .A left rostral maxillectomy was performed and the tumor was submitted for histological assessment .Histopathological diagnosis of the tissue revealed a feline inductive odontogenic tumor .There was no evidence of tumor recurrence 24 months after surgery.

Sporadic Worldwide “Clusters” Of Feed Driven Zilpaterol Identifications In Racing Horses: A Review And Analysis, Jacob Machin, Kimberly Brewer, Abelardo Morales Briceno, Clara Fenger, George Maylin, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Zilpaterol is a β2-adrenergic agonist medication approved in certain countries as a cattle feed additive to improve carcass quality. Trace amounts of Zilpaterol can transfer to horse feed, yielding equine urinary “identifications” of Zilpaterol. These “identifications” occur because Zilpaterol is highly bioavailable in horses, resistant to biotransformation and excreted as unchanged Zilpaterol in urine, where it has a 5 day or so terminal half-life.

In horses, urinary steady-state concentrations are reached 25 days (5 half-lives) after exposure to contaminated feed. Zilpaterol readily presents in horse urine, yielding clusters of feed related Zilpaterol identifications in racehorses. The first cluster, April …

Comparison Of Abdominal Computed Tomography To Ultrasound In The Diagnosis Of Canine Biliary Disease Manifesting As Acute Abdominal Signs, Shanna Christine Marroquin

Theses and Dissertations

Biliary diseases are uncommon, potentially fatal causes of acute abdomen in dogs. Little information is present comparing the performance of computed tomography (CT) to ultrasound in identifying canine biliary pathology. Thirty-five client-owned dogs presenting for acute abdomen signs received an abdominal ultrasound and contrast-enhanced abdominal CT. Two authors reviewed the randomized, anonymized CT and ultrasound studies. Twenty-eight dogs had biliary pathology and seven dogs serving as controls had no evidence of biliary disease. The final diagnoses of patients with biliary pathology included cholelithiasis, gallbladder mucoceles, cholangiohepatitis/cholangitis, extrahepatic biliary obstruction, gallbladder wall edema, gallbladder wall mass, and cystic mucosal hyperplasia. Computed …

Seasonal Variation And Tracking Of Climate Niche Of A Migratory Bird, Javier Gutiérrez Illán, Guiming Wang, D. Tommy King, Fred L. Cunningham

United States Department of Agriculture Wildlife Services: Staff Publications

Migratory birds inhabit different areas during breeding and non-breeding seasons. Depending on the time of the year, they may utilize different resources available in seasonal habitats, but also are subjected to changing climate regimes during their annual life cycle. Migratory birds may adopt ecological niche tracking to cope with different environmental conditions between breeding and non-breeding grounds. The American White Pelican (Pelecanus erythrorhynchos, hereafter ‘AWPE’) is a short-distance migrant between the Gulf of Mexico coastal regions (nonbreeding grounds) and the Northern Great Plains (breeding grounds) of Canada and the US. The American White Pelican is a piscivore, feeding …

Liquid Chromatography With Tandem Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Symone T. Whalin

Masters Theses & Specialist Projects

Bovine mastitis, caused by a wide array of pathogens, results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the bovine’s innate immune system and act as the first line of defense against mastitis. Only foundational research has been done on β-defensins’ ability to treat and prevent mastitis. There have been no analytical methods reported in the literature for analyzing β-defensins in bovine milk. This research aims to create an analytical approach to determine β-defensins in bovine milk. It is challenging to determine an analyte in a complex sample matrix, and milk is …

Time Course Investigation Of The Dermal Leukocyte Response To Lipoteichoic Acid In Chickens, Ian Gilbert

Animal Science Undergraduate Honors Theses

Lipoteichoic acid (LTA) is component of the cell wall of Gram-positive bacteria that stimulates inflammation during bacterial infection. However, few studies have investigated the in vivo immune response to LTA, and none of the in vivo studies done have been performed in birds. For this project, the pulp (a skin-derivative) of growing feathers (GFs) of chickens were used as a test site to investigate the in vivo effects of intradermally injected LTA. In Study 1, the pulp of 12 GFs of 11-week-old Light-brown Leghorn (LBL) males were injected with 10 μL of differing concentrations of LTA (0.1, 1.0, 10, 100 …

Barriers To Client Compliance And Understanding Of Heartworm Prevention In The Canine Patient, Emily Fischels

Honors College Theses

Dirofilaria immitis is a common and largely preventable mosquito-borne parasite that causes dirofilariasis, or heartworm disease, in a number of animal species. The necessity of heartworm prevention in the canine patient is undisputed and well-known among veterinary professionals, yet there are still many dogs not protected from this fatal parasite. Heartworm prevention traditionally requires a once-a-month administration of medication; therefore, its efficacy relies on owner compliance. The barriers to canines receiving heartworm prevention were assessed using a survey targeted at pet owners whose dogs have seen the veterinarian in the past year. The main barriers identified were inadequate client communication …

Relative Palatability And Efficacy Of Brodifacoum-25d Conservation Rodenticide Pellets For Mouse Eradication On Midway Atoll, Peter J. Kappes, Shane R. Siers, Israel L. Leinbach, Robert T. Sugihara, Wesley J. Jolley, Jonathan H. Plissner, Elizabeth N. Flint, Kelly L. Goodale, Gregg R. Howald

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive mice (Mus spp.) can negatively impact island species and ecosystems. Because fewer island rodent eradications have been attempted for mice compared to rats (Rattus spp.), less is known about efficacy and palatability of rodenticide baits for mouse eradications. We performed a series of bait acceptance and efficacy cage trials using a standard formulation of brodifacoum-based rodenticide on wild-caught mice from Sand Island, Midway Atoll, to help inform a proposed eradication there. Mice were offered ad libitum brodifacoum pellets along with various alternative food sources, and a “no choice” treatment group received only bait pellets. Mortality in the no choice …

Intercontinental Movement Of Highly Pathogenic Avian Influenza A(H5n1) Clade 2.3.4.4 Virus To The United States, 2021, Sarah N. Bevins, Susan A. Shriner, James C. Cumbee Jr., Krista E. Dilione, Kelly E. Douglass, Jeremy W. Ellis, Mary Lea Killian, Mia K. Torchetti, Julianna B. Lenoch

United States Department of Agriculture Wildlife Services: Staff Publications

We detected Eurasian-origin highly pathogenic avian influenza A(H5N1) virus belonging to the Gs/GD lineage, clade 2.3.4.4b, in wild waterfowl in 2 Atlantic coastal states in the United States. Bird banding data showed widespread movement of waterfowl within the Atlantic Flyway and between neighboring flyways and northern breeding grounds.

What Is The Prevalence Of Dirofilaria Immitis (Dog Heartworm) In Invasive Aedes Mosquitoes?, Leen Yousef, Johan Espinoza Corrales, Daniela H. Dolar, Taylor K. Trieu, Aisha Popalzai, Agamchat Dhami

Pacific Undergraduate Research and Creativity Conference (PURCC)

No abstract provided.

Evaluating The Microbial Quality And Use Of Antimicrobials In Raw Pet Foods, Leslie Pearl Cancio

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Raw meat-based diets (RMBDs) are emerging pet foods that pose food safety risks because of the potential presence of pathogens that could cause illness to humans. In this research, the microbial quality of select RMBD products sold by pet food companies online and the use of chemical antimicrobials to reduce the microbial load in chicken liver, a common RMBD ingredient, were evaluated.

Ground meat blends and livers from four animal species (beef, pork, chicken, turkey) were purchased from four online companies that delivers directly to consumers through parcel businesses. Products were procured at three different times during one year and …

Individual Trophic Niche Specialization In American Beaver (Castor Canadensis), Jimmy Taylor, Robert Francis, Scott Rush, Bronson Strickland, Guiming Wang

United States Department of Agriculture Wildlife Services: Staff Publications

The American beaver (Castor canadensis) has been described as a choosy generalist at the species/population scale, yet observational studies have shown little variation in diet among individuals. We compared isotopic values of δ¹³C or δ¹⁵N taken from hair of 32 beaver, representing seven colonies in northern Alabama, USA to determine 1) if colonies of beaver show overlap in isotopic niche width as a result of the similar use of food resources and 2) if individual trophic niche specialization occurs within colonies. Total Trophic Niche Width varied across the wetland with the widest being twice …

Intra-Articular Buprenorphine In Horses, Gabriel Castro-Cuellar Dr

LSU Master's Theses

Opioid drugs have the potential of provide local analgesia in inflamed joints. To date, morphine is the only opioid that has been tested for intra-articular (IA) administration in horses. Having an alternative drug, other than morphine, could widen the therapeutic options, particularly in cases of drug shortages or inaccessibility to specific drugs. The work presented in this dissertation reports the cytotoxic effects of buprenorphine on cultured equine chondrocytes, and the pharmacokinetic, pharmacodynamic and analgesic effects, of IA administered buprenorphine in horses with experimentally induced synovitis.

To evaluate the potential cytotoxic effects on equine cartilage, chondrocytes were obtained from normal equine …

Polydactyly In Cats, Rosa Benson, Cassie Morris, Leaha Mancuso, Bernardo Mesa

Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity

Polydactyly is a genetic condition in which a cat is born with more than the typical number of toes. It is a dominant genetic mutation and is fairly common in cats. (Class Project)

Editorial: Special Issue “Innovative Techniques And Approaches In The Control And Prevention Of Rabies Virus”, Amy T. Gilbert, Ryan M. Wallace, Charles E. Rupprecht

United States Department of Agriculture Wildlife Services: Staff Publications

Rabies is an ancient lethal scourge that has plagued humankind for centuries. Globally, 60,000 human deaths are estimated to occur each year from rabies virus (RABV) transmission in domestic dogs, mostly affecting children. While rabies is recognized as a neglected disease, there is cause for optimism in the context of growing global recognition, collaboration and commitment to advance a tripartite agenda to eliminate human deaths transmitted from rabid dogs by 2030, also known as “Zero By Thirty” (ZBT). Nevertheless, the ZBT goal must also confront competing challenge(s) of tracking and mitigating human morbidity and mortality during a global pandemic caused …

Can We Use Antipredator Behavior Theory To Predict Wildlife Responses To High-Speed Vehicles?, Ryan B. Lunn, Bradley Blackwell, Travis L. Devault, Esteban Fernández-Juricic

United States Department of Agriculture Wildlife Services: Staff Publications

Animals seem to rely on antipredator behavior to avoid vehicle collisions. There is an extensive body of antipredator behavior theory that have been used to predict the distance/time animals should escape from predators. These models have also been used to guide empirical research on escape behavior from vehicles. However, little is known as to whether antipredator behavior models are appropriate to apply to an approaching high-speed vehicle scenario. We addressed this gap by (a) providing an overview of the main hypotheses and predictions of different antipredator behavior models via a literature review, (b) exploring whether these models can generate quantitative …

A Novel Endo Gia™ Staple Cartridge For Canine Lung Lobectomies, Alissa St. Blanc

LSU Master's Theses

The canine lungs are well suited for ventilation, gas exchange, and gas transport. In diseased states, changes within bronchi and pulmonary parenchyma alter the blood-gas interface. Diagnostic workups to characterize pulmonary disease may include blood gas analysis, bronchoalveolar lavage, tracheobronchoscopy, radiography and/or computed tomography. Surgical pulmonary diseases include cysts, bullae, and blebs; congenital anomalies; consolidation or abscessation; bronchiectasis; lacerations; lung lobe torsions; and neoplasia. Surgical options may include laceration repair, partial or total lung lobectomy, and pneumonectomy. These options are most commonly utilized in cases that fail medical management. Lung lobectomies were originally performed using suture ligation. Stapling devices have …