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Articles 1141 - 1170 of 9777

Full-Text Articles in Veterinary Medicine

Long-Term Effect Of A Gnrh-Based Immunocontraceptive On Feral Cattle In Hong Kong, Rebecca Pinkham, Ka-Kei Koon, Jason To, Jason Chan, Flavie Vial, Matt Gomm, Douglas C. Eckery, Giovanna Massei Aug 2022

Long-Term Effect Of A Gnrh-Based Immunocontraceptive On Feral Cattle In Hong Kong, Rebecca Pinkham, Ka-Kei Koon, Jason To, Jason Chan, Flavie Vial, Matt Gomm, Douglas C. Eckery, Giovanna Massei

United States Department of Agriculture Wildlife Services: Staff Publications

Increasing human-wildlife conflicts worldwide are driving the need for multiple solutions to reducing “problem” wildlife and their impacts. Fertility control is advocated as a non-lethal tool to manage free-living wildlife and in particular to control iconic species. Injectable immunocontraceptives, such as GonaCon, stimulate the immune system to produce antibodies against the gonadotrophin-releasing hormone (GnRH), which in turn affects the release of reproductive hormones in mammals. Feral cattle (Bos indicus or Bos taurus) in Hong Kong are an iconic species whose numbers and impacts on human activities have increased over the last decade. Previous studies have proven that a …


Long-Term Effect Of A Gnrh-Based Immunocontraceptive On Feral Cattle In Hong Kong, Rebecca Pinkham, Ka-Kei Koon, Jason To, Jason Chan, Flavie Vial, Matt Gomm, Douglas C. Eckery, Giovanna Massei Aug 2022

Long-Term Effect Of A Gnrh-Based Immunocontraceptive On Feral Cattle In Hong Kong, Rebecca Pinkham, Ka-Kei Koon, Jason To, Jason Chan, Flavie Vial, Matt Gomm, Douglas C. Eckery, Giovanna Massei

United States Department of Agriculture Wildlife Services: Staff Publications

Increasing human-wildlife conflicts worldwide are driving the need for multiple solutions to reducing “problem” wildlife and their impacts. Fertility control is advocated as a non-lethal tool to manage free-living wildlife and in particular to control iconic species. Injectable immunocontraceptives, such as GonaCon, stimulate the immune system to produce antibodies against the gonadotrophin-releasing hormone (GnRH), which in turn affects the release of reproductive hormones in mammals. Feral cattle (Bos indicus or Bos taurus) in Hong Kong are an iconic species whose numbers and impacts on human activities have increased over the last decade. Previous studies have proven that a …


Synthesis And Characterization Of D5-Barbarin For Use In Barbarin-Related Research, Sucheta Kudrimoti, Jacob Machin, Adedamola S. Arojojoye, Samuel G. Awuah, Rodney Eisenberg, Clara Fenger, George Maylin, Andreas F. Lehner, Thomas Tobin Aug 2022

Synthesis And Characterization Of D5-Barbarin For Use In Barbarin-Related Research, Sucheta Kudrimoti, Jacob Machin, Adedamola S. Arojojoye, Samuel G. Awuah, Rodney Eisenberg, Clara Fenger, George Maylin, Andreas F. Lehner, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Based on structural similarities and equine administration experiments, Barbarin, 5-phenyl-2-oxazolidinethione from Brassicaceae plants, is a possible source of equine urinary identifications of aminorex, (R,S)-5-phenyl-4,5-dihydro-1,3-oxazol-2-amine, an amphetamine-related US Drug Enforcement Administration (DEA) controlled substance considered illegal in sport horses. We now report the synthesis and certification of d5-barbarin to facilitate research on the relationship between plant barbarin and such aminorex identifications. D5-barbarin synthesis commenced with production of d5-2-oxo-2-phenylacetaldehyde oxime (d5-oxime) from d5-acetophenone via butylnitrite in an ethoxide/ethanol solution. This d5-oxime was then reduced with lithium aluminum …


Ex Vivo Biomechanical Evaluation Of A Novel Screw For Tibial Plateau Leveling Osteotomy, William Seth Kettleman Aug 2022

Ex Vivo Biomechanical Evaluation Of A Novel Screw For Tibial Plateau Leveling Osteotomy, William Seth Kettleman

Theses and Dissertations

Twelve pairs of cadaveric pelvic limbs were harvested from skeletally mature dogs. Tibias were randomly assigned pairwise to two study groups: locking buttress screw fixation (LBS) and bone-screw-fastener fixation (BSF). A tibial plateau leveling osteotomy (TPLO) was performed. Each specimen was positioned in a servohydraulic testing machine to simulate physiological orientation and loading at the mid-point of the stance phase at the walk. Cyclic loading was performed for 30,000 cycles at 4Hz with a peak-load of 1000N. The cyclic test was then continued by stepwise increasing the peak-load at a rate of 75N per 500 cycles until failure of the …


Ex Vivo Biomechanical Comparison Of A Novel Compression Screw Fastener And Traditional Ao Cortical Bone Screw For Fixation Of A Simulated Slab Fracture In The Equine Third Carpal Bone, Allison Salinger Aug 2022

Ex Vivo Biomechanical Comparison Of A Novel Compression Screw Fastener And Traditional Ao Cortical Bone Screw For Fixation Of A Simulated Slab Fracture In The Equine Third Carpal Bone, Allison Salinger

Theses and Dissertations

Frontal plane slab fractures account for the majority of third carpal bone (C3) fractures in performance horses. Treatment is stabilization with an AO cortical screw. Complications are fragment splitting, fragment spinning, and irritation of dorsal soft tissue structures. A novel, headless, cannulated screw with interlocking threads (the Headless Compression Screw Fastener, HCSF) has been developed to resist multidirectional forces. Simulated C3 slab fractures were created in nine paired equine carpi. HCSF or AO cortical screws were loaded in shear to failure. Stiffness, maximum load to failure, and yield load was assessed in linear mixed models. No significant difference was detected …


Bacterial Chondronecrosis With Osteomyelitis In Broilers: Genomics, Phylogenomics, And Methods To Detect Specific Pathogens During Outbreaks., Abdulkarim Abdulaziz A. Shwani Aug 2022

Bacterial Chondronecrosis With Osteomyelitis In Broilers: Genomics, Phylogenomics, And Methods To Detect Specific Pathogens During Outbreaks., Abdulkarim Abdulaziz A. Shwani

Graduate Theses and Dissertations

Lameness is a major issue in animal welfare and the broiler industry. Bacterial chondronecrosis with osteomyelitis (BCO) is one of the main causes of lameness. Many staphylococcal species, including Staphylococcus agnetis isolate 908, have been isolated from the bones and blood of lame broilers at the University of Arkansas. Staphylococcus agnetis is a coagulase-variable, Gram-positive bacterial species that has been previously associated with subclinical or mild clinical cases of mastitis in dairy cattle. The annotated complete genome of hypervirulent strain 908 was published at NCBI. In this study, it has been compared to nine genomes we assembled for hypervirulent isolates …


In Vivo Efficacy Of Pyrantel Pamoate As A Post-Exposure Prophylactic For Rat Lungworm (Angiostrongylus Cantonensis), John Jacob, Argon Steel, Lisa Kaluna, Steven Hess, Israel Leinbach, Carmen Antaky, Robert Sugihara, Lindsey Hamilton, Peter Follett, Kathleen Howe, Steven Jacquier, Supakit Wongwiwatthananukit, Susan Jarvi Aug 2022

In Vivo Efficacy Of Pyrantel Pamoate As A Post-Exposure Prophylactic For Rat Lungworm (Angiostrongylus Cantonensis), John Jacob, Argon Steel, Lisa Kaluna, Steven Hess, Israel Leinbach, Carmen Antaky, Robert Sugihara, Lindsey Hamilton, Peter Follett, Kathleen Howe, Steven Jacquier, Supakit Wongwiwatthananukit, Susan Jarvi

United States Department of Agriculture Wildlife Services: Staff Publications

Rat lungworm (Angiostrongylus cantonensis) is a neurotropic nematode, and the leading cause of eosinophilic meningitis worldwide. The parasite is usually contracted through ingestion of infected gastropods, often hidden in raw or partially cooked produce. Pharmaceutical grade pyrantel pamoate was evaluated as a post-exposure prophylactic against A. cantonensis. Pyrantel pamoate is readily available over-the-counter in most pharmacies in the USA and possesses anthelmintic activity exclusive to the gastrointestinal tract (GIT). Administering pyrantel pamoate immediately after exposure should theoretically paralyze the larvae in the GIT, causing the larvae to be expelled via peristalsis without entering the systemic circulation. In this study, pyrantel …


The Role Of Temperature Variation On Macrophage Physiology And Function In Vitro In Mice, Chicken, And Fish, Intisar Abdullah Hassan Aug 2022

The Role Of Temperature Variation On Macrophage Physiology And Function In Vitro In Mice, Chicken, And Fish, Intisar Abdullah Hassan

Graduate Theses and Dissertations

Fever is an essential component of the immune response. Baseline body temperatures vary in different species, and fever represents increased temperature over baseline. Fever initiates and enhances immune responses as well as creates an environment in which the body has advantages over pathogens. Macrophages are often the first line of cells that come in contact with pathogens, asthey reside in and traffic through tissues. They are important for their engulfment of pathogens that results in the digestion of the pathogen, and they also produce nitric oxide and cytokines that contribute to immune responses in a variety of ways, including initiating …


Chondronecrosis With Osteomyelitis In Broilers: Bacterial Species Involvement And Influence Of Probiotics And Synbiotics, Amer Abdullah Hasan Aug 2022

Chondronecrosis With Osteomyelitis In Broilers: Bacterial Species Involvement And Influence Of Probiotics And Synbiotics, Amer Abdullah Hasan

Graduate Theses and Dissertations

Since 1945, the market for broiler production has evolved from a collection of smallholder poultry farms into an industry worth billions of dollars. Poultry production has not gone without challenges. Lameness in young broilers is presently a serious issue in the poultry industry for reasons of animal health and welfare, as well as significant lost production and revenue. Bacterial chondronecrosis with osteomyelitis (BCO) is a major form of lameness in broilers. This dissertation contains information about (i) the influence of Staphylococcus agnetis, Staphylococcus saprophyticus, Staphylococcus epidermidis and Enterococcus faecalis on inducing BCO lameness, (ii) evaluation of of probiotics for reducing …


Current Ecotoxicity Testing Needs Among Selected U.S. Federal Agencies, Patricia Ceger, Natalia Garcia-Reyero Vinas, David Allen, Elyssa Arnold, Raanan Bloom, Jennifer C. Brennan, Carol Clarke, Karen Eisenreich, Kellie Fay, Jonathan Hamm, Paula F.P. Henry, Katherine E. Horak, Wesley Hunter, Donna Judkins, Patrice Klein, Nicole Kleinstreuer, Kara Koehrn, Carlie A. Lalone, James P. Laurenson, Jessica K. Leet, Anna Lowit, Scott G. Lynn, Teresa Norberg-King, Edward J. Perkins, Elijah J. Petersen, Barnett A. Rattner, Catherine S. Sprankle, Thomas Steeger, Jim E. Warren, Sarah Winfield, Edward Odenkirchen Aug 2022

Current Ecotoxicity Testing Needs Among Selected U.S. Federal Agencies, Patricia Ceger, Natalia Garcia-Reyero Vinas, David Allen, Elyssa Arnold, Raanan Bloom, Jennifer C. Brennan, Carol Clarke, Karen Eisenreich, Kellie Fay, Jonathan Hamm, Paula F.P. Henry, Katherine E. Horak, Wesley Hunter, Donna Judkins, Patrice Klein, Nicole Kleinstreuer, Kara Koehrn, Carlie A. Lalone, James P. Laurenson, Jessica K. Leet, Anna Lowit, Scott G. Lynn, Teresa Norberg-King, Edward J. Perkins, Elijah J. Petersen, Barnett A. Rattner, Catherine S. Sprankle, Thomas Steeger, Jim E. Warren, Sarah Winfield, Edward Odenkirchen

United States Department of Agriculture Wildlife Services: Staff Publications

U.S. regulatory and research agencies use ecotoxicity test data to assess the hazards associated with substances that may be released into the environment, including but not limited to industrial chemicals, pharmaceuticals, pesticides, food additives, and color additives. These data are used to conduct hazard assessments and evaluate potential risks to aquatic life (e.g., invertebrates, fish), birds, wildlife species, or the environment. To identify opportunities for regulatory uses of non-animal replacements for ecotoxicity tests, the needs and uses for data from tests utilizing animals must first be clarified. Accordingly, the objective of this review was to identify the ecotoxicity test data …


Defining An Epidemiological Landscape That Connects Movement Ecology To Pathogen Transmission And Pace-Of-Life, Kezia Manlove, Mark Wilber, Lauren White, Guillaume Bastille-Rousseau, Anni Yang, Marie L.J. Gilbertson, Meggan E. Craft, Paul C. Cross, George Wittemyer, Kim M. Pepin Aug 2022

Defining An Epidemiological Landscape That Connects Movement Ecology To Pathogen Transmission And Pace-Of-Life, Kezia Manlove, Mark Wilber, Lauren White, Guillaume Bastille-Rousseau, Anni Yang, Marie L.J. Gilbertson, Meggan E. Craft, Paul C. Cross, George Wittemyer, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

Pathogen transmission depends on host density, mobility and contact. These components emerge from host and pathogen movements that themselves arise through interactions with the surrounding environment. The environment, the emergent host and pathogen movements, and the subsequent patterns of density, mobility and contact form an ‘epidemiological landscape’ connecting the environment to specific locations where transmissions occur. Conventionally, the epidemiological landscape has been described in terms of the geographical coordinates where hosts or pathogens are located. We advocate for an alternative approach that relates those locations to attributes of the local environment. Environmental descriptions can strengthen epidemiological forecasts by allowing for …


Effect Of A Glp-1 Mimetic On The Insulin Response To Oral Sugar Testing In Horses, Darko Stefanovski, Mary A Robinson, Andrew Van Eps Jul 2022

Effect Of A Glp-1 Mimetic On The Insulin Response To Oral Sugar Testing In Horses, Darko Stefanovski, Mary A Robinson, Andrew Van Eps

Faculty, Staff and Student Publications

BACKGROUND: Insulin dysregulation (ID) is the most important risk factor for the development of laminitis in horses and therapies to control it are needed.

HYPOTHESIS/OBJECTIVES: To assess the effects of a single dose of the synthetic GLP-1 analog exenatide on postprandial insulin dynamics. We hypothesized that exenatide would improve insulin sensitivity and lower postprandial blood insulin concentrations.

STUDY DESIGN: Randomized, crossover, experimental study.

ANIMALS: Six horses (3 mares, 3 geldings; 2 with normal insulin regulation [NIR] and 4 with mild ID).

METHODS: Horses completed both study arms: subcutaneous administration of exenatide (or no treatment) 30 min before an oral sugar …


Optimising Response To An Introduction Of African Swine Fever In Wild Pigs, Kim M. Pepin, Vienna R. Brown, Anni Yang, James C. Beasley, Raoul Boughton, Kurt C. Vercauteren, Ryan S. Miller, Sarah N. Bevins Jul 2022

Optimising Response To An Introduction Of African Swine Fever In Wild Pigs, Kim M. Pepin, Vienna R. Brown, Anni Yang, James C. Beasley, Raoul Boughton, Kurt C. Vercauteren, Ryan S. Miller, Sarah N. Bevins

United States Department of Agriculture Wildlife Services: Staff Publications

African swine fever virus (ASFv) is a virulent pathogen that threatens domestic swine industries globally and persists in wild boar populations in some countries. Persistence in wild boar can challenge elimination and prevent disease-free status, making it necessary to address wild swine in proactive response plans. In the United States, invasive wild pigs are abundant and found across a wide range of ecological conditions that could drive different epidemiological dynamics among populations. Information on the size of the control areas required to rapidly eliminate the ASFv in wild pigs and how this area should change with management constraints and local …


Amplification Of Black Vulture (Coragyps Atratus) Dna From Regurgitated Food Pellets, Daniel R. Taylor, Bryan M. Kluever, John S. Humphrey, Iona M. Hennessy, Amber Sutton, William E. Bruce, Antoinette J. Piaggio Jul 2022

Amplification Of Black Vulture (Coragyps Atratus) Dna From Regurgitated Food Pellets, Daniel R. Taylor, Bryan M. Kluever, John S. Humphrey, Iona M. Hennessy, Amber Sutton, William E. Bruce, Antoinette J. Piaggio

United States Department of Agriculture Wildlife Services: Staff Publications

Studies that rely on noninvasive collection of DNA for birds often use feces or feathers. Some birds, such as vultures, regurgitate undigested matter in the form of pellets that are commonly found under roost sites. Our research demonstrates that regurgitated pellets are a viable, noninvasive source of DNA for molecular ecology studies of vultures. Our objectives were to amplify 5 microsatellite loci designed for distinguishing Turkey Vultures (Cathartes aura) and Black Vultures (Coragyps atratus) in a single, multiplexed PCR, and to determine how long the target nuclear DNA persists after a vulture pellet is regurgitated and …


Evaluation Of Residual Antibacterial Effects On Canine Skin Surface And Hairs Following Treatment With Five Commercial Mousse Products Against Staphylococcus Pseudintermedius In Vitro, Chi-Yen Wu Jul 2022

Evaluation Of Residual Antibacterial Effects On Canine Skin Surface And Hairs Following Treatment With Five Commercial Mousse Products Against Staphylococcus Pseudintermedius In Vitro, Chi-Yen Wu

LSU Master's Theses

Topical products are essential treatments for canine superficial bacterial folliculitis. However, studies for commercial products typically evaluate antibacterial effects on hairs rather than the skin surface. In addition, the interference from the hair length has not been evaluated in these studies. Therefore, the study objective for this study was to evaluate the residual antibacterial effects of five mousse products, (1) 2% chlorhexidine and 2% miconazole, (2) 0.05% phytosphingosine, (3) 2% salicylic acid and 10% ethyl lactate, (4) 3% chlorhexidine and 0.5% climbazole, and (5) 2% chlorhexidine and 1% ketoconazole on both the skin surface and hairs in both short- and …


Seasonal Climatic Niche And Migration Movements Of Double-Crested Cormorants, D. Tommy King, Guiming Wang, Fred L. Cunningham Jul 2022

Seasonal Climatic Niche And Migration Movements Of Double-Crested Cormorants, D. Tommy King, Guiming Wang, Fred L. Cunningham

United States Department of Agriculture Wildlife Services: Staff Publications

Avian migrants are challenged by seasonal adverse climatic conditions and energetic costs of long-distance flying. Migratory birds may track or switch seasonal climatic niche between the breeding and non-breeding grounds. Satellite tracking enables avian ecologists to investigate seasonal climatic niche and circannual movement patterns of migratory birds. The Double-crested Cormorant (Nannopterum auritum, hereafter cormorant) wintering in the Gulf of Mexico (GOM) migrates to the Northern Great Plains and Great Lakes and is of economic importance because of its impacts on aquaculture. We tested the climatic niche switching hypothesis that cormorants would switch climatic niche between summer and winter …


Understanding How High Levels Of Noise Affect The Equine Auditory System, Shelby Brown Jul 2022

Understanding How High Levels Of Noise Affect The Equine Auditory System, Shelby Brown

Ursidae: The Undergraduate Research Journal at the University of Northern Colorado

Abstract

Mounted shooting is becoming a very popular sport within the equestrian community, exposing many horses to extreme levels of noise that the animals would not be exposed to otherwise. I selected this project because it is important for me to know how this hobby that I personally enjoy is impacting the health and well-being of my equine partners. Veterinarians can provide an elementary answer by clapping and checking for a physical reaction. However, that does not tell the client much other than the animal responded to one auditory cue.

It is important to understand how firearm exposure could affect …


Solar Radiation And Soil Moisture Drive Tropical Forest Understory Responses To Experimental And Natural Hurricanes, J. Aaron Hogan, Joanne M. Sharpe, Ashley Van Beusekom, Sarah Stankavich, Samuel Matta Carmona, John E. Bithorn, Jamarys Torres-Díaz, Grizelle González, Jess K. Zimmerman, Aaron B. Shiels Jul 2022

Solar Radiation And Soil Moisture Drive Tropical Forest Understory Responses To Experimental And Natural Hurricanes, J. Aaron Hogan, Joanne M. Sharpe, Ashley Van Beusekom, Sarah Stankavich, Samuel Matta Carmona, John E. Bithorn, Jamarys Torres-Díaz, Grizelle González, Jess K. Zimmerman, Aaron B. Shiels

United States Department of Agriculture Wildlife Services: Staff Publications

Tropical forest understory regeneration occurs rapidly after disturbance with compositional trajectories that depend on species availability and environmental conditions. To predict future tropical forest regeneration dynamics, we need a deeper understanding of how pulse disturbance events, like hurricanes, interact with environmental variability to affect understory demography and composition. We examined fern and sapling mortality, recruitment, and community composition in relation to solar radiation and soil moisture using 17 years of forest dynamics data (2003–2019) from the Canopy Trimming Experiment in the Luquillo Experimental Forest, Puerto Rico. Solar radiation increased 150% and soil moisture increased 40% following canopy trimming of experimental …


Comparative Susceptibility Of Eastern Cottontails And New Zealand White Rabbits To Classical Rabbit Haemorrhagic Disease Virus (Rhdv) And Rhdv2, Fawzi Mohamed, Thomas Gidlewski, Mary L. Berninger, Heather M. Petrowski, Alexa J. Bracht, Carla Bravo De Rueda, Roger W. Barrette, Meredith Grady, Emily S. O'Hearn, Charles E. Lewis, Karen E. Moran, Tracy L. Sturgill, Lorenzo Capucci, J. Jeffrey Root Jul 2022

Comparative Susceptibility Of Eastern Cottontails And New Zealand White Rabbits To Classical Rabbit Haemorrhagic Disease Virus (Rhdv) And Rhdv2, Fawzi Mohamed, Thomas Gidlewski, Mary L. Berninger, Heather M. Petrowski, Alexa J. Bracht, Carla Bravo De Rueda, Roger W. Barrette, Meredith Grady, Emily S. O'Hearn, Charles E. Lewis, Karen E. Moran, Tracy L. Sturgill, Lorenzo Capucci, J. Jeffrey Root

United States Department of Agriculture Wildlife Services: Staff Publications

Rabbit haemorrhagic disease virus (RHDV) is associated with high morbidity and mortality in the European rabbit (Oryctolagus cuniculus). In 2010, a genetically distinct RHDV named RHDV2 emerged in Europe and spread to many other regions, including North America in 2016. Prior to this study it was unknown if eastern cottontails (ECT(s); Sylvilagus floridanus), one of the most common wild lagomorphs in the United States, were susceptible to RHDV2. In this study, 10 wild-caught ECTs and 10 New Zealand white rabbits (NZWR(s); O. cuniculus) were each inoculated orally with either RHDV (RHDVa/GI.1a; n = 5 per species) or RHDV2 (a recombinant …


Genomic Tools Reveal Complex Social Organization Of An Invasive Large Mammal (Sus Scrofa), Chelsea L. Titus, Courtney F. Bowden, Timothy J. Smyser, Stephen L. Webb, James C. Beasley Jun 2022

Genomic Tools Reveal Complex Social Organization Of An Invasive Large Mammal (Sus Scrofa), Chelsea L. Titus, Courtney F. Bowden, Timothy J. Smyser, Stephen L. Webb, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

A comprehensive understanding of sociality in wildlife is vital to optimizing conservation and management efforts. However, sociality is complicated, especially for widely distributed species that exhibit substantive behavioral plasticity. Invasive wild pigs (Sus scrofa), often representing hybrids of European wild boar and domestic pigs, are among the most adaptable and widely distributed large mammals. The social structure of wild pigs is believed to be similar to European wild boar, consisting of matriarchal groups (sounders) and solitary males. However, wild pig social structure is understudied and largely limited to visual observations. Using a hierarchical approach, we incorporated genomic tools …


Hepatotoxicity In Acute And Subchronic Oral Gavage Of Ethanolic Extract Of Christia Vespertilionis (L.F.) Bakh. F. Leaf In Male Sprague Dawley Rats, Nurul S.A.S., Nurul S.A.S., Hazilawati H., Rosly S.M., Farhan Hanif M.R., Abdullahi A., Shanmugavelu S., Noordin M.M., Mazlina M., Azrina A., Norhaizan M.E. Jun 2022

Hepatotoxicity In Acute And Subchronic Oral Gavage Of Ethanolic Extract Of Christia Vespertilionis (L.F.) Bakh. F. Leaf In Male Sprague Dawley Rats, Nurul S.A.S., Nurul S.A.S., Hazilawati H., Rosly S.M., Farhan Hanif M.R., Abdullahi A., Shanmugavelu S., Noordin M.M., Mazlina M., Azrina A., Norhaizan M.E.

The Thai Journal of Veterinary Medicine

Increased interest in Christia vespertilionis (L.f.) Bakh. f. leaves is due to their real potential of their health-enhancing properties. This study aims to evaluate the possible in vivo toxicity in 14-day acute and 90-day subchronic oral toxicity of ethanolic extract Christia vespertilionis (L.f.) Bakh. f. in male Sprague Dawley rats. In the acute study, the rats were divided into control, 5% DMSO (vehicle) and 2000 mg/kg groups. For the subchronic toxicity study, a total of 30 rats were divided into five groups consisting of a control, 5% DMSO (vehicle), low dose (75 mg/kg), medium dose (125 mg/kg) and high dose …


Recombinant Scfv Inhibit Newcastle Disease Virus In Vitro Infection, Sanfeng Peng, Lintian Yu, Ying He, Dongyang Chen, Li Wang, Xueliang Zhou, Long Xie, Yangqing Lu Jun 2022

Recombinant Scfv Inhibit Newcastle Disease Virus In Vitro Infection, Sanfeng Peng, Lintian Yu, Ying He, Dongyang Chen, Li Wang, Xueliang Zhou, Long Xie, Yangqing Lu

The Thai Journal of Veterinary Medicine

Newcastle disease (ND) is an avian respiratory disease caused by the Newcastle disease virus (NDV) and is prevalent worldwide but lacks a reliable treatment to surmount it. The single-chain fragment variable (scFv) antibody is the smallest functional unit in an immunoglobulin retaining antigen-binding activities. Previous studies have demonstrated that by acting against the NDV phosphoprotein, scFv is capable of neutralizing the virus in infected cells. In this study, two anti-NDV scFv expressing cassettes driven by CAG promoter and EF1α promoter, respectively, were constructed using the piggyBac transposon plasmid backbone. The recombinant plasmids were transfected into DF-1 and 293T cells and …


Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate, Bo Dong, Bo Dong, Xiaodong Zhang, Xiaodong Zhang, Yafei Song, Chengyu Li, Gaoqiang Zhang, Weiming Lin Jun 2022

Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate, Bo Dong, Bo Dong, Xiaodong Zhang, Xiaodong Zhang, Yafei Song, Chengyu Li, Gaoqiang Zhang, Weiming Lin

The Thai Journal of Veterinary Medicine

FCoV viruses exhibit great genetic diversity, leading to the presence of FIPV-causing variants. Current molecular evolution analysis and genetic variation studies of FCoV in China are predominately focused on gene encoding the spike protein or other structural proteins, while few studies have evaluated genetic variations in nonstructural FCoV genes, which can play an important role in disease pathogenesis. In this study, the gene encoding the open reading frame (ORF) 7b nonstructural FCoV protein of the Chinese Fujian strain FJLY20201 was amplified from the ascitic fluid of a Chinese domestic cat infected with FIPV and compared with ORF 7b from previously …


Yield, Composition, Fatty Acid Profile And Cla Content Of Milk From Goats Fed With Different Levels Of Opf, Pitunart Noosen, Ulia Renfelia Baysi Jun 2022

Yield, Composition, Fatty Acid Profile And Cla Content Of Milk From Goats Fed With Different Levels Of Opf, Pitunart Noosen, Ulia Renfelia Baysi

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the effect of different inclusion levels of OPF in total mixed ration (TMR)-based diet on yield, composition, FA profile and CLA content of milk in dairy goats. Six nulliparous female dairy goats (Saanen x Thai native, 19-21 months old, first lactation) were randomly set according to a 3x3 Latin square design with 2 replications for receiving diets consisted of 0% OPF (OPF0), 20% OPF (OPF20) and 40% OPF (OPF40). The average weight of the goats was 32.48±1.30 kg (mean±s.d.). The findings of this study showed that dry matter intake (DMI) and all …


Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam, Tran Duc Hoan, Doan Thi Thao, Nguyen Thi Huong Giang, Nguyen Thi Thu Huyen, Nguyen Thi Thu Huyen Jun 2022

Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam, Tran Duc Hoan, Doan Thi Thao, Nguyen Thi Huong Giang, Nguyen Thi Thu Huyen, Nguyen Thi Thu Huyen

The Thai Journal of Veterinary Medicine

To identify further the infectivity, genetic diversity and molecular characteristics of the porcine epidemic diarrhea virus (PEDV). 286 samples of the intestinal tissue of pigs with severe diarrhea were assessed from 5 provinces in the north of Vietnam. The full-length ORF3 gene of 20 representative field strains from 36 farms in 5 provinces was sequenced and analyzed. The prevalence of PEDV was 90.56% and was detected in 259 of 286 samples, with 94.44% (34 of 36) of pig farms harboring the disease. Based on the phylogenetic analysis of the ORF3 genes, our isolates all fell into group G2 (variants) and …


Investigation Of The Microstructure And Mechanical Properties Of The Domestic Cat’S Paw Pads, Ye Mei Jun 2022

Investigation Of The Microstructure And Mechanical Properties Of The Domestic Cat’S Paw Pads, Ye Mei

The Thai Journal of Veterinary Medicine

Domestic cats have a biological function such as cushioning when they are jumping or walking which is mainly due to the biological coupling system formed in the long-term evolution process of nature, including the trunk-leg-paw pad structure. Among them, the paw pad, as the only part of the body that directly contacts with the ground in the process of movement, plays a key role in its biological function. Hence, in order to clarify the micro structure, mechanical properties and functional characteristics of the paw pad and reveal the relationship between it and the domestic cat’s biological function, this paper has …


Optimal Infusion Rate Of Hypertonic Saline To Encephalic Vessel Vasodilatation Using Magnetic Resonance Imaging In Normovolemic Dogs, Yoshiki Murakami, Kenji Tsukano, Haruyuki Hirata, Hiroshi Koie, Kazuyuki Suzuki Jun 2022

Optimal Infusion Rate Of Hypertonic Saline To Encephalic Vessel Vasodilatation Using Magnetic Resonance Imaging In Normovolemic Dogs, Yoshiki Murakami, Kenji Tsukano, Haruyuki Hirata, Hiroshi Koie, Kazuyuki Suzuki

The Thai Journal of Veterinary Medicine

This study aimed to investigate, using magnetic resonance imaging, whether continuous hypertonic saline (HSS) infusion induces a superior vasodilating effect on the encephalic vessels compared to bolus infusion in dogs. The relative cross-sectional area of superior sagittal sinus increased to 1.96±0.09 and 1.69±0.31 times of the pre-value in the continuous and bolus groups, respectively, at t=30 min (p<0.001). However, the relative cross-sectional area of superior sagittal sinus was larger in the continuous group than that in the bolus group (p<0.001). The results suggest that continuous infusion of HSS might be superior to bolus infusion in inducing vasodilatation of the encephalic circulation.


Oxygenated Dressing Quickens Skin Wound Healing Of Bama Miniature Pigs, Yingqiu Wang, Limin Qiao, Hong An, Zhenhao Zhang, Yan Zheng, Chang Chen, Jingrui Zhang, Xiangguo Wang Jun 2022

Oxygenated Dressing Quickens Skin Wound Healing Of Bama Miniature Pigs, Yingqiu Wang, Limin Qiao, Hong An, Zhenhao Zhang, Yan Zheng, Chang Chen, Jingrui Zhang, Xiangguo Wang

The Thai Journal of Veterinary Medicine

Oxygen is a vital factor in the process of wound healing and is involved in collagen synthesis, synthesis and the release of growth factors, angiogenesis and epidermal cell contraction. Hence, local oxygen control of wounds plays an important role in skin wound healing. Herein, we developed a novel oxygenated dressing for the treatment of skin wound healing in Bama miniature pigs. The oxygenated dressing was mainly composed of potassium iodide, glucose, glucose oxidase, polyvinyl alcohol, polypropylene, polyvinylpyrrolidone and water. The oxygenated dressing significantly quickens the healing of full-thickness skin wounds in Bama miniature pigs and promotes the proliferation of epidermal …


Feline Inductive Odontogenic Tumor In A Young Cat, Chakkarin Satthathum, Sunee Kunakornsawat, Chaiyan Kasorndorkbua Jun 2022

Feline Inductive Odontogenic Tumor In A Young Cat, Chakkarin Satthathum, Sunee Kunakornsawat, Chaiyan Kasorndorkbua

The Thai Journal of Veterinary Medicine

A 6-month-old, 4.45 kg, intact, male domestic shorthair cat was presented with a gingival mass located on the left rostral maxilla and second left upper premolar tooth. Intraoral x-ray of the left rostral maxilla revealed left maxilla bone lysis with a unilocular cystic lesion. The cytology result revealed an epithelial cell tumor .A left rostral maxillectomy was performed and the tumor was submitted for histological assessment .Histopathological diagnosis of the tissue revealed a feline inductive odontogenic tumor .There was no evidence of tumor recurrence 24 months after surgery.


Assessment Of Abdominal Fat Distribution By Computed Tomography In Obese Dogs, Yunseo Jang, Hyemin Na, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee Jun 2022

Assessment Of Abdominal Fat Distribution By Computed Tomography In Obese Dogs, Yunseo Jang, Hyemin Na, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee

The Thai Journal of Veterinary Medicine

Obesity is a risk factor for canine health but there has been no study on the tendency of body fat accumulation naturally occurring in obese dogs. This retrospective observational study aims to evaluate the tendency to accumulate visceral and subcutaneous fat using computed tomography (CT) in naturally occurring obese dogs. The study included 15 dogs with a body condition score of 8 or 9 that underwent abdominal CT scans. Total fat area (TA), visceral fat area (VA), subcutaneous fat area (SA) and body area (BA) were measured by non-contrast transverse image at the third lumbar vertebra (L3) and the sixth …