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Articles 15571 - 15600 of 30246
Full-Text Articles in Mechanical Engineering
Effect Of Powder Particle Size On The Fabrication Of Ti-6al-4v Using Laser Metal Deposition From Elemental Powder Mixture, Xueyang Chen, Lei Yan, Wei Li, Frank W. Liou, Joseph William Newkirk
Effect Of Powder Particle Size On The Fabrication Of Ti-6al-4v Using Laser Metal Deposition From Elemental Powder Mixture, Xueyang Chen, Lei Yan, Wei Li, Frank W. Liou, Joseph William Newkirk
Mechanical and Aerospace Engineering Faculty Research & Creative Works
Direct LMD (laser metal deposition) was used to fabricate thin-wall Ti-6Al-4V using the powder mixture of Ti-6 wt.%Al-4 wt.%V. SEM (scanning electron microscopy), OM (optical microscopy) and EDS (energy dispersive spectroscopy) were employed to examine the chemical composition and microstructure of the as-deposited sections. Vickers hardness tests were then applied to characterize the mechanical properties of the deposit samples which were fabricated using pre-mixed elemental powders. The EDS line scans indicated that the chemical composition of the samples was homogenous across the deposit. After significant analysis, some differences were observed among two sets of deposit samples which varied in the …
Aerodynamic And Modal Analyses Of Small Scale Horizontal Axis Wind Turbine With Various Numbers Of Blade Design, Emile Maroha
Aerodynamic And Modal Analyses Of Small Scale Horizontal Axis Wind Turbine With Various Numbers Of Blade Design, Emile Maroha
College of Graduate Studies: Theses & Dissertations
With the global energy demand rising to unprecedented numbers, the need for alternative energy sources is ever prevalent. Wind energy is the most alternative renewable sources today due to its year-round availability and pollution-free nature. Horizontal Axis Wind Turbines (HAWT) are the most popular because of their higher efficiency. The aerodynamic characteristics and vibration of small scale HAWT with various numbers of blade design have been investigated in this numerical study in order to improve its performance. SolidWorks was used for designing CAD models, and ANSYS software was used to study the dynamic flow around the turbine as well as …
Enhancing Project Management With Lean World Class Manufacturing In Construction, Jacob Randles
Enhancing Project Management With Lean World Class Manufacturing In Construction, Jacob Randles
College of Graduate Studies: Theses & Dissertations
In construction, the goal of commercial projects is to turn a profit. This is achieved by minimizing inputs and maximizing outputs while building a product that meets the customer’s perception of value. Value is determined through pre-negotiated specifications, plans, and material quality. Even in U.S. military construction, the unit executing the construction wants to complete the task on time and on budget, which can be difficult to do. Studies on project completion showcase a surprisingly high rate of failure in reaching the aforementioned “on time, on budget” goal. It is important to understand the nuances and details of U.S. Army …
Complex Capillary Fluidic Phenomena For Passive Control Of Liquids In Low-Gravity Environments, Logan Torres
Complex Capillary Fluidic Phenomena For Passive Control Of Liquids In Low-Gravity Environments, Logan Torres
Maseeh Summer Undergraduate Research Experience
In an effort to further apply the recent results of puddle jumping research, we seek to expand the oblique droplet impact studies of others by exploiting large liquid droplets in the near weightless environment of a drop tower. By using the spontaneous puddle jump mechanism, droplets of volumes 1 mL ≤ V ≤ 3 mL with corresponding Weber numbers of We ≈ 1 are impinged on surfaces inclined in the range 40° ≤ α ≤ 80° (measured from the horizontal plane). Impact surface wetting characteristics exhibit static contact angles θstatic = 165 ± 5°. All impacts result in complete rebound. …
Design For Fractal Grid Generated Turbulence, Moira Gion
Design For Fractal Grid Generated Turbulence, Moira Gion
Maseeh Summer Undergraduate Research Experience
Fractal geometry can be used to find organization or order in something that appears to be in disorder, or chaos. Turbulence is viewed as one place where fractal geometry can be found in nature. The irregularity in fluid flow is dependent on the initial conditions. In the past, the idea of self-similarity has been used to develop statistical theories of energy cascade in a turbulent flow. Recent studies have done experiments on obstructing flows with fractal objects to observe intermittency, (or self-similarity) within a turbulent flow. Experiments on fractal grid generated turbulence has been performed by other research groups, so …
Finite Element And Probabilistic Analysis Of Soft Tissue Structures Of The Human Lumbar Spine, Dana Joseph Coombs
Finite Element And Probabilistic Analysis Of Soft Tissue Structures Of The Human Lumbar Spine, Dana Joseph Coombs
Electronic Theses and Dissertations
Human lumbar spine mechanics are influenced by soft tissue structures. Understanding and properly modeling these structures can help determine pathology, treatment, and implant design and performance. Finite element models of the L4-L5 level of the lumbar spine are often used, which include a representation of the intervertebral disc and spinal ligaments. Validation of these models are typically based on torque rotation data from a single subject or the models use average properties reported in literature. However, experimental testing reports variation up to 40% in ligament stiffness and even greater variability for annulus fibrosis properties. Probabilistic approaches enable consideration of the …
Methods And Implementation Of Fluid-Structure Interaction Modeling Into An Industry-Accepted Design Tool, Donn R. Sederstrom
Methods And Implementation Of Fluid-Structure Interaction Modeling Into An Industry-Accepted Design Tool, Donn R. Sederstrom
Electronic Theses and Dissertations
Fluid-structure interaction (FSI) modeling is a method by which fluid and solid domains are coupled together to produce a single result that cannot be produced if each physical domain was evaluated individually. The work presented in this dissertation is a demonstration of the methods and implementation of FSI modeling into an industry-appropriate design tool. Through utilizing computationally inexpensive equipment and commercially available software, the studies presented in this work demonstrate the ability for FSI modeling to become a tool used broadly in industry.
To demonstrate this capability, the cases studied purposely include substantial complexity to demonstrate the stability techniques required …
An Assessment Of The Validity Of The Kinetic Model For Liquid-Vapor Phase Change By Examining Cryogenic Propellants, Kishan Bellur
An Assessment Of The Validity Of The Kinetic Model For Liquid-Vapor Phase Change By Examining Cryogenic Propellants, Kishan Bellur
Dissertations, Master's Theses and Master's Reports
Evaporation is ubiquitous in nature and occurs even in a microgravity space envi- ronment. Long term space missions require storage of cryogenic propellents and an accurate prediction of phase change rates. Kinetic theory has been used to model and predict evaporation rates for over a century but the reported values of accommodation coefficients are highly inconsistent and no accurate data is available for cryogens. The proposed study involves a combined experimental and computational approach to ex- tract the accommodation coefficients. Neutron imaging is used as the visualization technique due to the difference in attenuation between the cryogen and the metallic …
Drive Signal Development For The Thermoacoustic Loudspeaker, Troy Bouman
Drive Signal Development For The Thermoacoustic Loudspeaker, Troy Bouman
Dissertations, Master's Theses and Master's Reports
Carbon nanotube thermophones can create acoustic waves from 1 Hz to 100 kHz. The thermoacoustic effect that allows for this non-vibrating sound source is naturally inefficient. Prior efforts have not explored their true efficiency (i.e. the ratio of the total acoustic power to the electrical input power). All previous works have used the ratio of sound pressure to input electrical power. A method for true power efficiency measurement is shown using a fully anechoic technique. True efficiency data are presented for five different drive signal processing techniques: standard alternating current (AC), direct current added to AC (DCAC), amplitude modulation of …
Finite Element Modeling Of Active And Passive Behavior Of The Human Tibialis Anterior: A Preliminary Approach, David Joda
Dissertations, Master's Theses and Master's Reports
This research project serves as exploratory work in the field of computational human biomechanics. A connection between muscular force and intramuscular pressure (IMP) has been uncovered that could prove invaluable in medical diagnostics as a method to circumvent the use of electromyography.
Preliminary finite element simulations were conducted to model the human tibialis anterior muscle in passive lengthening and active contraction. These simulations, totaling over 50 unique runs, utilized a novel constitutive model developed within the IMP research group. Volumetric strain, reaction forces, and pressure gradients were compared to data acquired from ongoing in vivo human experiments. A mechanism for …
Design And Fabrication Of A Suspension Rig, Abhijith Geo Philip
Design And Fabrication Of A Suspension Rig, Abhijith Geo Philip
Dissertations, Master's Theses and Master's Reports
Owing to its placement under the vehicle body, the suspension system is one of the parts of the vehicle which are difficult to comprehend completely, albeit being a vital component. For demonstrating the working of a suspension system, a physical model in which one can actually see the movement of all the components would be a great tool. A Mustang II independent front suspension system was purchased from Heidts Engineering Performance and a rig that can emulate some of the real world situations of vehicle suspension action was designed and fabricated. All the components are accessible and conditions like bounce, …
Evaluation Of Angular Distribution Of Incident Field At The Transmission Loss Window In Michigan Tech’S Reverberant Chamber, Abhishek Thyagarajan
Evaluation Of Angular Distribution Of Incident Field At The Transmission Loss Window In Michigan Tech’S Reverberant Chamber, Abhishek Thyagarajan
Dissertations, Master's Theses and Master's Reports
Transmission Loss prediction accuracy is highly dependent on a good understanding of the angular distribution of incident field on the panel. Traditionally, the incident field has been assumed to be either completely random (equal probability of incidence at all angles from 0° - 90°) or field incidence (where the field is assumed to be completely diffuse between 0° - 78°). Studies1-3 have shown that these models are not completely representative of the incident field. This incident field is studied in the Michigan Tech Transmission Loss suite using two different methods in this study; beamforming and acoustic intensity. The beamforming method …
Experimental Study Of The Flow Of Ferrofluid In A Porous Media Under A Magnetic Field, Muskaan Khurana
Experimental Study Of The Flow Of Ferrofluid In A Porous Media Under A Magnetic Field, Muskaan Khurana
Dissertations, Master's Theses and Master's Reports
This research presents results from a laboratory-scale experimental setup that was designed to visualize the behavior of ferrofluid percolation through a porous media. Ferrofluids are colloidal suspensions made of magnetic particles of a few nanometers and stabilized in carrier liquids like water or mineral oil. Ferrofluids get magnetized and align themselves in the direction of a magnetic field when a field gradient is applied.
With the help of this experiment we investigate the viability of controlling fluid flow in porous medium by a magnetic field in vicinity. The experiments show that ferrofluids can be used as a transporting media to …
Finite Element Analysis And Topology Optimization Of Differential Case And Control Arm For Static And Fatigue Loading, Pankaj N. Kalan
Finite Element Analysis And Topology Optimization Of Differential Case And Control Arm For Static And Fatigue Loading, Pankaj N. Kalan
Dissertations, Master's Theses and Master's Reports
Optimization of the automobile components can result in a significant decrease in vehicle weight, increase in fuel efficiency and reduction in environmental damage. For example, the lightweight vehicle production will save over 4.4×109 GJ of energy and 4.1×108 tons of CO2 over a 10 year period, compared to the current non-light weight vehicles. This equates to 890 billion miles or 42 billion gallons of gas savings (Wu 2016). This study evaluates the weight reduction possible by using manual and automatic (using Optistruct software) topology optimization processes. Optimization of a vehicle differential case done in this study shows that a weight …
Competition Vehicle Based Intake Manifold Design, Joshua N. Matiash
Competition Vehicle Based Intake Manifold Design, Joshua N. Matiash
Dissertations, Master's Theses and Master's Reports
A competitive vehicle in Formula SAE needs to be easy for unskilled drivers to extract the maximum performance from. This requires a predictable and manageable torque curve. This report details the development of an intake manifold for a Formula SAE car from a vehicle-based approach to produce this manageable and predictable torque. The current vehicle was instrumented and driven on a representative track to determine the usage of available torque. Based on these findings an ideal torque curve was chosen that favored increased torque at upper engine speed ranges and decreased torque at lower engine speed ranges. A 1-D engine …
Analyses Of 476 Mhz And 952 Mhz Crab Cavities For Jlab Electron Ion Collider, Hyekyoung Park, A. Castilla, J. R. Delayen, S. U. De Silva, V. Morozov
Analyses Of 476 Mhz And 952 Mhz Crab Cavities For Jlab Electron Ion Collider, Hyekyoung Park, A. Castilla, J. R. Delayen, S. U. De Silva, V. Morozov
Physics Faculty Publications
Center for Accelerator Science at ODU has designed, fabricated and successfully tested a crab cavity for MEIC at Jefferson Lab*. This proof of principle cavity was based on the earlier MEIC design which used 748.5 MHz RF system. The updated MEIC design** utilizes the components from PEP-II. It results in the change on the bunch repetition rate of stored beam to 476.3 MHz. The ion ring collider will eventually require 952.6 MHz crab cavity. This paper will present the analyses of crab cavities of both 476 MHz and 952 MHz options. It compares advantages and disadvantages of the options which …
Anisotropic Electrical Response Of Carbon Fiber Reinforced Composite Materials, Mohammad Faisal Haider
Anisotropic Electrical Response Of Carbon Fiber Reinforced Composite Materials, Mohammad Faisal Haider
Theses and Dissertations
Composites materials are often subjected to multi-physical conditions in different applications where, in addition to mechanical loads, they also need to sustain other types of loads such as electrical currents. The multi-physical behavior of composites needs to be understood and analyzed to facilitate new multi-functional material design. An essential first step towards this goal is to understand how multi-physics properties depend on local details (e.g. micro-structure). Composite materials have heterogeneous electrical properties (carbon/epoxy) at the local level that can be different at the global level. To conduct the multi-physics study, the electrical signal is employed to the composite sample for …
Techno Economical Analysis Of Solid Oxide Iron-Air Redox Battery For Power Generation And Energy Storage, Farzana Yasmeen
Techno Economical Analysis Of Solid Oxide Iron-Air Redox Battery For Power Generation And Energy Storage, Farzana Yasmeen
Theses and Dissertations
The largest source of greenhouse gas emissions in the United States is from burning fossil fuels for electricity, heat, and transportation. Due to increasing environmental constraints associated with greenhouse gas emissions and uncertainty in the supply of fossil fuels, power systems of the future will become more reliable on renewable power source. To overcome the intermittency issue of renewable power, a promising cost effective electricity-storage systems is vital. The U.S. Department of Energy (DOE) has established detailed cost targets for energy storage system. One of the targets is capital cost which should be under $150/kWh for new technology. Another target …
Automated Tool Selection And Tool Path Planning For Free-Form Surfaces In 3-Axis Cnc Milling Using Highly Parallel Computing Architecture, Andrey Balabokhin
Automated Tool Selection And Tool Path Planning For Free-Form Surfaces In 3-Axis Cnc Milling Using Highly Parallel Computing Architecture, Andrey Balabokhin
Theses and Dissertations
This research presents a methodology to automatically select cutters and generate tool paths for all stages in 3-axis CNC Milling of free-form surfaces. Tools are selected and tool paths are planned in order to minimize the total machining time. A generalized cutter geometry model is used to define available cutters and an arbitrary milling surface is initially defined by a triangular mesh.
The decisions made by process engineers in selecting cutting geometry and generating tool paths for milling dramatically influence the final result. Often, the resulting tool path is non-optimal, because the engineers cannot consider all the available information. However, …
Modeling Radionuclide Diffusion Using Moose A Multiscale, Multiphysics Platform, Jonathon Gardner
Modeling Radionuclide Diffusion Using Moose A Multiscale, Multiphysics Platform, Jonathon Gardner
Theses and Dissertations
Very durable ceramic waste forms have been proposed which offer longterm stability by binding certain radionuclides in their specific crystalline network. Moreover, multiple such ceramic phases can be tailored to contain specific radionuclides generated in the fuel cycle. Many such candidate ceramic forms are in the early stages of development with limited data. Modeling and simulation including modeling of diffusion can inform and provide direction to ongoing fabrication and experimental efforts. Material properties important in modeling can be obtained through laboratory measurement where available and atomistic simulation. Since diffusion occurs over multiple scales and follows multiple pathways, multiscale modeling is …
A Shipboard Instrument For Underway Estimates Of Marine N2 Fixation, Weiyi Tang
A Shipboard Instrument For Underway Estimates Of Marine N2 Fixation, Weiyi Tang
Link Foundation Ocean Engineering and Instrumentation Fellowship Reports
Marine N2 fixation converts nitrogen gas into ammonia and accounts for the largest external nitrogen input to the oceans1. Since large portions of the global oceans are nitrogen deficient, marine N2 fixation can relieve the nitrogen limitation, thereby increasing the growth of phytoplankton and stimulating biological uptake of atmospheric carbon dioxide2,3,4. However, the magnitude and distribution of marine N2 fixation are poorly constrained5. In addition, the environmental controls on marine N2 fixation remain elusive6. This situation is in part attributable to the limited number of observations and poor coverage of marine N2 fixation measurements. Current observations rely on discrete sampling …
Anatomy, Implant Selection And Placement Influence Spine Mechanics Associated With Total Disc Replacement, Justin F.M. Hollenbeck
Anatomy, Implant Selection And Placement Influence Spine Mechanics Associated With Total Disc Replacement, Justin F.M. Hollenbeck
Electronic Theses and Dissertations
Through aging and injury, the intervertebral disc of the lumbar spine can undergo degeneration, leading to collapse of the vertebrae and low back pain, a symptom that affects half the adult population in any given year. In an effort to reduce low back pain, total disc replacement treatment removes the degenerated disc, restores natural height and lordosis of the segment, and preserves motion at the joint. Patient anatomy, implant selection, and implant placement play significant roles in a patient's outcomes after total disc replacement surgery. Thus, the objective of the work presented in this thesis was to develop a suite …
A Nocsae Drop Test/ 3d-Fem Study On The Relationship Between Kinematic Response Of The Head, Head Impact Contact Pressure (Hicp) And Kinetic Response Of The Brain To Delineate The Risk Of Traumatic Brain Injuries (Tbi)., Raghavendra Krishna Tej Bhamidipati
A Nocsae Drop Test/ 3d-Fem Study On The Relationship Between Kinematic Response Of The Head, Head Impact Contact Pressure (Hicp) And Kinetic Response Of The Brain To Delineate The Risk Of Traumatic Brain Injuries (Tbi)., Raghavendra Krishna Tej Bhamidipati
Dissertations, Master's Theses and Master's Reports
How many times have you seen a person slip and fall down and had a good laugh about it? How many times we slip and fall down on the floor and got up and walked away thinking everything is normal? Probably we might wonder “Are drops which occur at a small height dangerous?” Yes, they can be. Brain injuries are main reason for the fatality in youth [1], and low level falls are one of the most neglected and understated injuries.
The main objective of this study is to study the response of the brain for low level falls from …
Pulsed Laser Cutting Of Magnesium-Calcium For Biodegradable Stents, M. P. Sealy, Y. B. Guo, J. F. Liu, C. Li
Pulsed Laser Cutting Of Magnesium-Calcium For Biodegradable Stents, M. P. Sealy, Y. B. Guo, J. F. Liu, C. Li
Department of Mechanical and Materials Engineering: Faculty Publications
There is growing interests in the use of biodegradable magnesium implants for cardiovascular and pulmonary applications such as stents. Magnesium is a metal that has the ability to gradually dissolve and absorb into the human body after implantation. There is very little work discussing the relationship between process parameters and cut quality of magnesium stents by laser cutting. The objective of this research is to determine the effect of laser cutting conditions including peak laser power and cutting speed of a millisecond range pulsed laser on kerf geometry, surface topography, surface roughness, and microstructure. An assessment on the experimental work …
Impact Of Patient Factors On Operative Duration During Laparoscopic Cholecystectomy: Evaluation From The National Surgical Quality Improvement Program Database, Bethany Rose Lowndes, Cornelius A. Thiels, Elizabeth B. Habermann, Juliane Bingener, M. Susan Hallbeck, Denny Yu
Impact Of Patient Factors On Operative Duration During Laparoscopic Cholecystectomy: Evaluation From The National Surgical Quality Improvement Program Database, Bethany Rose Lowndes, Cornelius A. Thiels, Elizabeth B. Habermann, Juliane Bingener, M. Susan Hallbeck, Denny Yu
Department of Mechanical and Materials Engineering: Faculty Publications
BACKGROUND: Patient factors impact laparoscopic cholecystectomy (LC) difficulty, specifically operative duration. This study quantifies the impact of patient factors on LC duration.
METHODS: The national surgery database (American College of Surgeons National Surgical Quality Improvement Program) was reviewed for all elective LC for biliary colic from 2005 to 2013. Multivariate general linear model and logistic regression were used to evaluate patient factors as predictors of operative duration greater than 60 minutes, adjusted for resident involvement and cholangiography.
RESULTS: A total of 24,099 LC met inclusion criteria. Regression analysis found procedure duration greater than 60 minutes was less likely for patients …
A Comparative Study On Machining Capabilities Of Wet And Dry Nanoscale Electro-Machining, Muhammad P. Jahan, Kamlakar P. Rajurkar, Ajay P. Malshe
A Comparative Study On Machining Capabilities Of Wet And Dry Nanoscale Electro-Machining, Muhammad P. Jahan, Kamlakar P. Rajurkar, Ajay P. Malshe
Department of Mechanical and Materials Engineering: Faculty Publications
Presently, the nano scale electro-machining (nano-EM) process has been demonstrated in both the liquid and air dielectric mediums, which are known as wet and dry nano-EM respectively. In the current study, two important aspects of the nano-EM have been investigated: the minimum possible feature dimension and mass fabrication capability of nano-EM. Firstly, the investigation has been done on the capability of machining graphene at atomic scale with focus on obtaining smallest possible nano-feature using the wet nano-EM. Secondly, the ability of the nano-EM process for the fabrication of arrays of nano-holes has been investigated using dry nano-EM. It was found …
Bioink Properties Before, During And After 3d Bioprinting, Katja Holzl, Shengmao Lin, Liesbeth Tytgat, Sandra Van Vlierberghe, Linxia Gu, Aleksandr Ovsianikov
Bioink Properties Before, During And After 3d Bioprinting, Katja Holzl, Shengmao Lin, Liesbeth Tytgat, Sandra Van Vlierberghe, Linxia Gu, Aleksandr Ovsianikov
Department of Mechanical and Materials Engineering: Faculty Publications
Bioprinting is a process based on additive manufacturing from materials containing living cells. These materials, often referred to as bioink, are based on cytocompatible hydrogel precursor formulations, which gel in a manner compatible with different bioprinting approaches. The bioink properties before, during and after gelation are essential for its printability, comprising such features as achievable structural resolution, shape fidelity and cell survival. However, it is the final properties of the matured bioprinted tissue construct that are crucial for the end application. During tissue formation these properties are influenced by the amount of cells present in the construct, their proliferation, migration …
Integration Of Flow Studies For Robust Selection Of Mechanoresponsive Genes, Nataly Maimari, Ryan M. Pedrigi, Alessandra Russo, Krysia Broda, Rob Krams
Integration Of Flow Studies For Robust Selection Of Mechanoresponsive Genes, Nataly Maimari, Ryan M. Pedrigi, Alessandra Russo, Krysia Broda, Rob Krams
Department of Mechanical and Materials Engineering: Faculty Publications
Blood flow is an essential contributor to plaque growth, composition and initiation. It is sensed by endothelial cells, which react to blood flow by expressing >1000 genes. The sheer number of genes implies that one needs genomic techniques to unravel their response in disease. Individual genomic studies have been performed but lack sufficient power to identify subtle changes in gene expression. In this study, we investigated whether a systematic meta-analysis of available microarray studies can improve their consistency.
We identified 17 studies using microarrays, of which 6 were performed in vivo and 11 in vitro. The in vivo studies were …
Development Of A Synthetic Gene Network To Modulate Gene Expression By Mechanical Forces, Zoltan Kis, Tania Rodin, Asma Zafar, Zhangxing Lai, Grace Freke, Oliver Fleck, Armando Del Rio Hernandez, Leila Towhidi, Ryan M. Pedrigi, Takayuki Homma, Rob Krams
Development Of A Synthetic Gene Network To Modulate Gene Expression By Mechanical Forces, Zoltan Kis, Tania Rodin, Asma Zafar, Zhangxing Lai, Grace Freke, Oliver Fleck, Armando Del Rio Hernandez, Leila Towhidi, Ryan M. Pedrigi, Takayuki Homma, Rob Krams
Department of Mechanical and Materials Engineering: Faculty Publications
The majority of (mammalian) cells in our body are sensitive to mechanical forces, but little work has been done to develop assays to monitor mechanosensor activity. Furthermore, it is currently impossible to use mechanosensor activity to drive gene expression. To address these needs, we developed the first mammalian mechanosensitive synthetic gene network to monitor endothelial cell shear stress levels and directly modulate expression of an atheroprotective transcription factor by shear stress. The technique is highly modular, easily scalable and allows graded control of gene expression by mechanical stimuli in hard-to-transfect mammalian cells. We call this new approach mechanosyngenetics. To insert …
Comparison Between Direct And Reverse Electroporation Of Cells In Situ: A Simulation Study, Leila Towhidi, Delaram Khodadadi, Nataly Maimari, Ryan M. Pedrigi, Henry Ip, Zoltan Kis, Brenda R. Kwak, Tatiana W. Petrova, Mauro Delorenzi, Rob Krams
Comparison Between Direct And Reverse Electroporation Of Cells In Situ: A Simulation Study, Leila Towhidi, Delaram Khodadadi, Nataly Maimari, Ryan M. Pedrigi, Henry Ip, Zoltan Kis, Brenda R. Kwak, Tatiana W. Petrova, Mauro Delorenzi, Rob Krams
Department of Mechanical and Materials Engineering: Faculty Publications
The discovery of the human genome has unveiled new fields of genomics, transcriptomics, and proteomics, which has produced paradigm shifts on how to study disease mechanisms, wherein a current central focus is the understanding of how gene signatures and gene networks interact within cells. These gene function studies require manipulating genes either through activation or inhibition, which can be achieved by temporarily permeabilizing the cell membrane through transfection to deliver cDNA or RNAi. An efficient transfection technique is electroporation, which applies an optimized electric pulse to permeabilize the cells of interest. When the molecules are applied on top of seeded …