Veterinary Medicine Commons^™

Impact Of Black Soldier Fly (Hermetia Illucens) Fresh Meal On The Growth Performance, Digestive Enzymes, Hematology, And Intestinal Histology Of Cork Fish (Channa Striata), Boby Muslimin, Danang Yonarta, Eny Heriyati, Helmizuryani Helmizuryani, Meika Puspita Sari, Dewi Apriyanti, Rahmat Kurniawan

Turkish Journal of Veterinary & Animal Sciences

Black Soldier Fly (BSF) is an alternative ingredient for Fish Meal (FM) which is expected to reduce aquaculture operational costs and positively impact fish growth and health. The effect of BSF fresh meal on growth response, digestibility, intestinal hematology, and intestinal histology of cork fish was investigated. The BSF experiment with mixed percentages as treatments, by 20%, 50%, 80%, and 100%, was repeated four times. Furthermore, there were 50 individuals/m3 cork fish with an average weight of 1.6 ± 0.05 g distributed in each treatment. An increase in BSF influences a significant decrease in the Relative Growth Weight (RGW) and …

Evaluation Of Different Stocking Densities On Fattening Performance, Slaughter, Carcass, Meat Quality Characteristics, And Fatty Acid Composition In New Zealand Rabbits, Büşra Yaranoğlu, Mustafa Hi̇lmi̇ Yaranoğlu, Sali̇ha Uysal, Adnan Adi̇l Hi̇şmi̇oğullari

Turkish Journal of Veterinary & Animal Sciences

The aim of the research was to investigate the effects of different stocking densities on fattening performance, slaughter, carcass, meat quality characteristics, and fatty acid composition in New Zealand rabbits raised in cage conditions and also determine the ideal housing frequency so that yields were not adversely affected. In the study, 36 New Zealand rabbits were used. After weaning, the fattening was started and rabbits were put into cages at different stocking densities as 1 rabbit/cage, 2 rabbits/cage, and 3 rabbits/ cage. The live weight of rabbits and feed consumption data were taken every week. At the end of 8 …

Expression Of Hif-1Α, Caspase-3, P53, Hsp70, Hsp90, And Nrf2 In The Canine Spleen With Siderofibrotic Nodules: An Immunohistochemical And Immunofluorescence Perspective On Apoptosis, Ferroptosis, And Oxidative Stress, Ali Babanezhad-Gajouti, Amir Amniattalab

Turkish Journal of Veterinary & Animal Sciences

Splenic siderofibrotic nodules (SFNs) are benign tiny yellow-brownish foci found in dogs. Their pathogenesis is unclear whereas trauma and hemorrhage are assumed to be the possible causative factors. The spleen of 73 Iranian dogs of the mixed breed was examined for possible splenomegaly with SFNs. Ultrasonography, blood biochemistry, pathology, and immunohistochemistry revealed splenomegaly (n = 7), splenomegaly with SFNs (n = 6) and normal spleens (n = 60). There was no significant difference between the prevalence of splenomegaly with SFN among the examined dogs and their age or sex (p > 0.05). Ultrasonography represented a significant increase (p ≤ 0.01) in …

An Evaluation Of Canine Inguinal Hernias Containing The Uterus: Clinical Experience Of Four Cases (2017-2022) And Literature Review, Fi̇rdevs Bi̇nli̇, Fati̇h Büyükbudak, İpek İnan, Volkan Ferahoğlu, Anil Gürkan Aksu, Murat Findik, Serhan Serhat Ay

Turkish Journal of Veterinary & Animal Sciences

The aim of this study is to identify and summarize the evidence and risk factors of canine inguinal hernia containing the uterus (Inguinal Hysterocele; IH) etiology by presenting our clinical experience with a literature review. A total of 18 canine IH cases were evaluated in the study. Of these, four have not yet been published anywhere, two have been published before, and 12 were obtained through a literature review. According to the findings, 81.3% of the cases were small/medium races, 83.3% were ≥5 years old, and 72.7% were multiparous. Totally 83.3% of the cases were chronic and 72.2% of the …

Comparison Of Bacterial Profile And Antibiotic Susceptibility Isolated From Surgical Site After Ventral Midline And Lateral Flank Approaches Of Ovariohysterectomy In Queens And Bitches, Ebru Karakaya Bi̇len, Serpi̇l Kahya Demi̇rbi̇lek, Öznur Yilmaz, Muhammet Ali̇ Karadağ, Havva Aner

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to identify the bacterial profile and antimicrobial susceptibility from surgical site cultures in queens and bitches undergoing ovariohysterectomy via ventral midline and lateral flank approaches. Healthy 22 queens and 21 bitches were assigned randomly either ventral midline or lateral flank approach for routine ovariohysterectomy. Surgical site samples were collected before the surgery and on the 1$^{st}$, 2$^{nd}$ , and 3 $^{rd}$days after the surgery for microbiological analysis. A total of 70 different strains were isolated from 50 (29%) of 172 samples. In both queens and bitches the total number of bacteria isolated from the …

Stereological Investigation Of Medulla Oblongata Volume In Norduz Sheep, Ozan Ertaş, Gamze Çakmak

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to stereologically examine the medulla oblongata volume of Norduz sheep in the Gürpınar District of Van Province. Upon receiving news from the sections in the Gürpınar region, we went to the region. A total of 12 Norduz sheep skulls, 6 females and 6 males, were received from the Norduz sheep slaughter in the region. After the brains of Norduz sheep were dissected, they were fixed as a whole in formaldehyde for 1 week. Medulla oblongatas were separated from the brain and kept in formaldehyde for 1 week. Tissues obtained by sequential random sampling from …

Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron

Turkish Journal of Veterinary & Animal Sciences

Mastitis is a contagious disease that causes significant milk loss and serious economic damage in dairy animals. Streptococcus agalactiae causes clinical and subclinical mastitis in animals and infections in humans, especially adults, and newborns. This study aimed to confirm the identification of S. agalactiae isolates by PCR in buffalo milk; to determine the antibiotic resistance and virulence genes by multiplex PCR. The material of this study consisted of 30 (12%) S. agalactiae strains identified by phenotypic, biochemical, and PCR methods (rRNA-16S) from 250 isolates with bacterial growth from milk samples of buffaloes with mastitis. In addition, the Kirby-Bauer disc diffusion …

Effect Of Feeding Frequency On Growth, Fecundity, And Meat Quality Of Abant Trout (Salmo Abanticus Tortonese, 1954) Broodstock, Mehmet Zeki̇ Alkan, Nadi̇r Başçinar

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the growth data, egg production and changes in the biochemical structure of meat of Abant trout depending on feeding frequency (once a day, twice a day, three times a day, four times a day) in order to contribute to the economy through the private sector. All fish in the groups were planned to be of similar weight at the beginning of the study. Individual fish weights were measured at the beginning of the study, and the average was found to be 224.87 ± 0.69 g. At the end of the study, the weights …

In Vitro Antimicrobial And Antibiofilm-Forming Activities Of Origanum Munzurense Plant Extract On Some Foodborne Gram-Negative Bacteria, Hali̇l Yalçin, Esra Zeybek, Oktay Özkan, Asim Kart

Turkish Journal of Veterinary & Animal Sciences

lants are an important source for the discovery of antimicrobial formulations due to the many biologically active components they contain. In this study, in vitro antimicrobial and antibiofilm forming activities of ethanolic extract of Origanum munzurense were investigated against some strains of foodborne pathogenic bacteria. To investigate the antimicrobial activity, the minimal inhibitory concentration values against the selected bacteria and antibiofilm-forming activity were tested with the liquid microdilution method and microplate method, respectively. MICs of Origanum munzurense extract against the bacteria tested are 32 mg/mL for Escherichia coli O157: H7 ATCC 35150, 32 mg/mL for Escherichia coli O157: H7 ATCC …

The First Data Of A Newly Recorded Native Goat Genotype Called Manavlı In Türkiye: Growth Traits Of Kids, Aykut Asim Akbaş, Mustafa Saatci, Özkan Elmaz, Can Meti̇n Yazici

Turkish Journal of Veterinary & Animal Sciences

This study aimed to define growth traits of kids named Manavlı goat from six flocks under the breeder?s conditions in Denizli Province. The average birth weights of the kids were 3.63 kg, 4.28 kg, 3.85 kg, 4.11 kg, 3.83 kg, and 3.64 kg for the six flocks, respectively. The average live weights of male and female kids on 60th, 90th, and 150th day of age were 16.50 kg, 22.35 kg, and 34.82 kg and 15.33 kg, 20.14 kg, and 32.06 kg, respectively (p < 0.05). While the average live weights of single kids on 60th, 90th were 16.70 kg and 21.96 kg, the values for twins were 15.13 kg and 21.03 kg, respectively (p < 0.05). Body measurements of male kids such as height at withers, rump height, rump width, body length, heart girth, head length, ear length, tail length, and front and back wrist girth were measured as 70.26 cm, 70.21 cm, 16.23 cm, 66.80 cm, 70.11 cm, 19.51 cm, 22.16 cm, 18.59 cm, 11.85 cm, and 11.57 cm, respectively, on the 150th day of age. This study is the first research related to growth traits of Manavlı kids, for which no features have been reported to date. It was thought that the results obtained will help to characterise the Manavlı goat and will provide a database for the following studies.

The Devil You Know And The Devil You Don’T: Current Status And Challenges Of Bovine Tuberculosis Eradication In The United States, Daniel J. O'Brien, Tyler C. Thacker, Liliana C.M. Salvador, Anthony G. Duffiney, Suelee Robbe‑Austerman, Mark S. Camacho, Jason E. Lombard, Mitchell V. Palmer

United States Department of Agriculture Wildlife Services: Staff Publications

Having entered into its second century, the eradication program for bovine tuberculosis (bTB, caused by Mycobacterium bovis) in the United States of America occupies a position both enviable and daunting. Excepting four counties in Michigan comprising only 6109 km2 (0.06% of US land area) classified as Modified Accredited, as of April 2022 the entire country was considered Accredited Free of bTB by the US Department of Agriculture for cattle and bison. On the surface, the now well-described circumstances of endemic bTB in Michigan, where white-tailed deer (Odocoileus virginianus) serve as a free-ranging wildlife maintenance host, may appear to be …

Evaluation Of The Virulence Potential Of Avian Pathogenic Escherichia Coli Isolated From Broiler Breeders With Colibacillosis In Mississippi, Jiddu Joseph

Theses and Dissertations

Avian pathogenic Escherichia coli (APEC) is a bacterium that is responsible for colibacillosis in birds. However, information about broiler breeder APEC isolates is limited, but the data is critical due to the transfer of this bacteria down the production pyramid to progenies resulting in high mortality. Therefore, we evaluated the phenotypic virulence characteristics of 28 isolates using embryo lethality and day-old chick challenge assays. Also, the in vitro adhesion and invasion potential of selected nine isolates were identified. Results showed more than 1/3^rd of the isolates were highly virulent and the virulence increased as the number of virulence-associated genes …

Nutritional Strategies To Reduce Salmonella Enteritidis Infections In Laying Hens: Prebiotic, Probiotic, And Precision Biotics, Ishab Poudel

Theses and Dissertations

Salmonella Enteritidis (SE) infections in laying hens is a significant cause of foodborne illness linked to the consumption of contaminated eggs. This dissertation encompasses novel strategies aimed to explore appropriate in-vitro and in-vivo models to evaluate the effects of these novel strategies to reduce SE in laying hens. Firstly, we investigated the potential probiotics, prebiotics, and essential oil combinations to reduce SE in-vitro. The results showed that probiotics and prebiotics are equally effective to reduce SE. In subsequent studies, we evaluated the role of vaccination history on cecal microbiota and SE infections with various doses of oral SE challenge. …

Loaded Lipid Emulsified Volatile Anesthetics In Canine Primary Hepatocytes, Patricia De Carvalho Ibrahim Obeid

Theses and Dissertations

In the 19th century, halothane hepatitis became a sensitive and well-known subject in human anesthesiology due to the production of a noxious metabolite further discovered, trifluoroacetic acid. Subsequently, isoflurane, enflurane, and desflurane were also investigated for potentially causing hepatitis through the same metabolite. Sevoflurane, however, does not generate trifluoroacetic acid and is quickly conjugated and excreted.

For more than four decades these anesthetics have been experimentally developed for intravenous injection by having added either a lipid or fluorocarbon-based carrier to produce general anesthesia with less drug and faster onset of action. The use of intravenous emulsified halogenated anesthetics as an …

Comparison Of Aging Techniques In Wild Bottlenose Dolphins (Tursiops Truncatus) Of Unknown Age From The Mississippi Sound., Christa Elizabeth Barrett

Theses and Dissertations

Age determination of bottlenose dolphins (Tursiops truncatus) is a critical tool in understanding their health. There are many methods of aging dolphins including morphometrics, counting growth layer groups (GLGs) of teeth, comparing dentin to pulp cavity ratio on dental radiographs, analysis of the pectoral flipper radiographs, and epigenetic testing. The most common method for aging toothed cetaceans is the counting of GLGs in the teeth. Since there are many challenges associated with aging dolphins based on the counting of GLGs, a processing technique that results in better resolution of GLGs is needed. This is a two-part study that …

The Effect Of An Aiming Device On The Accuracy Of Humeral Transcondylar Screw Placement, Katherine Neal

Theses and Dissertations

Canine cadaveric forelimbs (136) were randomly assigned to either treatment (aiming device) or control (fluoroscopic-guided) groups, and to either diplomate or resident for humeral transcondylar screw placement. Each limb underwent a transcondylar humeral screw placement from a lateral to medial direction. Digital radiography was used to evaluate screw trajectory, measured as deviation from the epicondylar line, and eccentricity on the humeral condyle. Procedure time, difference in outcome based on experience level, and complications were recorded. In the right limb, screw trajectory with the aiming device was decreased compared to fluoroscopy. There was no difference of accuracy between leg laterality with …

A Literature Review Of The Psychosocial Impacts On Livestock Producers And Veterinary Responders Involved With Depopulation During And After An Animal Health Emergency, Beth Galles

Capstone Experience: Master of Public Health

A vital aspect of an animal health emergency in the United States is removing and disposing of diseased and healthy animals through depopulation. It is frequently required to prevent the spread of diseases that affect human health or economic stability. Production animals are eventually slaughtered, but this does not negate the human-animal bond felt between these animals and their caretakers. There is increasing recognition that participation in depopulation may be a traumatic event requiring care for the individuals involved, including livestock producers and veterinarians. Despite this, limited resources are available for these individuals when an animal health emergency occurs. This …

Leveraging Eco-Evolutionary Models For Gene Drive Risk Assessment, Matthew A. Combs, Andrew J. Golnar, Justin M. Overcash, Alun L. Lloyd, Keith R. Hayes, David A. O'Brochta, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

Engineered gene drives create potential for both widespread benefits and irreversible harms to ecosystems. CRISPR-based systems of allelic conversion have rapidly accelerated gene drive research across diverse taxa, putting field trials and their necessary risk assessments on the horizon. Dynamic processbased models provide flexible quantitative platforms to predict gene drive outcomes in the context of system-specific ecological and evolutionary features. Here, we synthesize gene drive dynamic modeling studies to highlight research trends, knowledge gaps, and emergent principles, organized around their genetic, demographic, spatial, environmental, and implementation features. We identify the phenomena that most significantly influence model predictions, discuss limitations of …

Assessing The Effects Of A Mixed Eimeria Spp. Challenge On Performance, Intestinal Integrity, And The Gut Microbiome Of Broiler Chickens, Danielle Graham, Victor Petrone-Garcia, Xochitl Hernandez-Velasco, Makenly E. Coles, Marco A. Juarez-Estrada, Juan D. Latorre, Jianmin Chai, Stephanie Shouse, Jiangchao Zhao, Aaron Forga, Roberto Señas-Cuesta, Lauren Laverty, Kristen Martin, Carolina Trujillo-Peralta, Ileana Loeza, Latasha Gray, Billy M. Hargis, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

A mixed Eimeria spp. challenge model was designed to assess the effects of challenge on broiler chicken performance, intestinal integrity, and the gut microbiome for future use to evaluate alternative strategies for controlling coccidiosis in broiler chickens. The experimental design involved broiler chickens divided into two groups: a control group (uninfected) and a positive control group, infected with Eimeria acervulina (EA), Eimeria maxima (EM), and Eimeria tenella (ET). At day-of-hatch, 240 off-sex male broiler chicks were randomized and allocated to one of two treatment groups. The treatment groups included: (1) Non-challenged (NC, n = 5 replicate pens); and (2) challenged …

Harnessing Autoimmunity With Dominant Self-Peptide: Modulating The Sustainability Of Tissue-Preferential Antigen-Specific Tregs By Governing The Binding Stability Via Peptide Flanking Residues, Youwei Lin, Shun Sakuraba, Chandirasegaran Massilamany, Jayagopala Reddy, Yoshimasa Tanaka, Sachiko Miyake, Takashi Yamamura

School of Veterinary and Biomedical Sciences: Faculty Publications

Sensitization to self-peptides induces various immunological responses, from autoimmunity to tumor immunity, depending on the peptide sequence; however, the underlying mechanisms remain unclear, and thus, curative therapeutic options considering immunity balance are limited. Herein, two overlapping dominant peptides of myelin proteolipid protein, PLP136-150 and PLP139-151, which induce different forms of experimental autoimmune encephalomyelitis (EAE), monophasic and relapsing EAE, respectively, were investigated. Mice with monophasic EAE exhibited highly resistant to EAE re-induction with any encephalitogenic peptides, whereas mice with relapsing EAE were susceptible, and progressed, to EAE re-induction. This resistance to relapse and reinduction in monophasic EAE mice was associated with …

The Knowns And Unknowns Of Cardiac Autoimmunity In Viral Myocarditis, Kiruthiga Mone, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Myocarditis can result from various infectious and non‐infectious causes that can lead to dilated cardiomyopathy (DCM) and heart failure. Among the infectious causes, viruses are commonly suspected. But the challenge is our inability to demonstrate infectious viral particles during clinical presentations, partly because by that point, the viruses would have damaged the tissues and be cleared by the immune system. Therefore, viral signatures such as viral nucleic acids and virus-reactive antibodies may be the only readouts pointing to viruses as potential primary triggers of DCM. Thus, it becomes hard to explain persistent inflammatory infiltrates that might occur in individuals affected …

Devoting Continuous Efforts To Innovations And Development Of Ruminant Livestock Life-Cycle Nutrition Program, Zhixiong He, Zhaomin Hu, Hongsheng Wang, Jinzhen Jiao, Xuefeng Han, Yong Liu, Shaoxun Tang, Chuanshe Zhou, Min Wang, Zhiliang Tan

Bulletin of Chinese Academy of Sciences (Chinese Version)

Ruminant livestock such as cattle and sheep can provide high-quality beef, mutton and milk, which are high-quality protein sources meeting the increasingly diverse food consumption needs for human being. Developing high-quality herbivorous animal husbandry is a concrete practice of practicing the “Greater Food” approach. The precise supply of forages and concentrates meeting the requirements of all nutrients in the whole life cycle is vital for cost saving and efficiency increasing of ruminant livestock industry, which is an important way to high-quality development. The whole life cycle nutrition is developed on the basis of traditional ruminant nutrition and new technologies made …

Nerve Transfer For Restoration Of Lower Motor Neuron-Lesioned Bladder, Urethral, And Anal Sphincter Function In A Dog Model. Part 3. Nicotinic Receptor Characterization, Nagat Frara, Mary F. Barbe, Dania Giaddui, Danielle S. Porreca, Alan S. Braverman, Ekta Tiwari, Attia Ahmad, Justin M. Brown, Benjamin R. Johnston, Stanley F. Bazarek, Michael R. Ruggieri

Department of Neurology Faculty Papers

Very little is known about the physiological role of nicotinic receptors in canine bladders, although functional nicotinic receptors have been reported in bladders of many species. Utilizing in vitro methods, we evaluated nicotinic receptors mediating bladder function in dogs: control (9 female and 11 male normal controls, 5 sham operated), Decentralized (9 females, decentralized 6–21 mo), and obturator-to-pelvic nerve transfer reinnervated (ObNT-Reinn; 9 females; decentralized 9–13 mo, then reinnervated with 8–12 mo recovery). Muscle strips were collected, mucosa-denuded, and mounted in muscle baths before incubation with neurotransmitter antagonists, and contractions to the nicotinic receptor agonist epibatidine were determined. Strip response …

Evaluating Vaccine Management Strategies For Edwardsiella Ictaluri Infections In Zebrafish (Danio Rerio), Brandy Malbrough

LSU Master's Theses

Aquaculture is a globally important industry that faces significant challenges due to infectious diseases, which can result in substantial financial losses. In 2011, a new strain of Edwardsiella ictaluri emerged as a major pathogen affecting zebrafish in the ornamental fish industry, leading to high mortality rates and posing a severe threat to the sector. Vaccines provide potential benefits for disease prevention in aquaculture, particularly live-attenuated vaccines (LAVs), which induce strong host immune responses. Bacterial attenuation for vaccine development has evolved from serial passage to more targeted genetic modification techniques. However, regulatory constraints limit the availability of licensed LAVs for aquaculture. …

Predicted Structure And Functional Roles Of Alphaherpesvirus Tegument Protein Ul37 In Virion Morphogenesis And Innate Immunity, Therese Marie Collantes

LSU Doctoral Dissertations

Several members of the Family Herpesviridae are clinically significant pathogens, affecting humans and a wide range of animals. Alphaherpesviruses infect epithelial cells at the primary site of infection and subsequently invade adjacent sensory neurons. Infectious bovine rhinotracheitis (IBR) is an economically important disease caused by bovine herpesvirus 1 (BoHV-1), which belongs to the subfamily Alphaherpesvirinae, genus Varicellovirus. To date, vaccination is the only preventive measure against IBR. Understanding determinants of herpesvirus replication and innate immune regulation is important to help further develop vaccines that are efficacious and incapable of establishing latency in the host nervous system. The alphaherpesvirus …

Naturally Occurring Highly Pathogenic Avian Influenza Virus H5n1 Clade 2.3.4.4b Infection In Three Domestic Cats In North America During 2023, Sarah J. Sillman, Mary Drozd, Duan S. Loy, Seth P. Harris

School of Veterinary and Biomedical Sciences: Faculty Publications

The Eurasian strain of highly pathogenic avian influenza (HPAI) H5N1 is a devastating pathogen for birds that also has the capacity to infect mammals. This report describes the presentation, clinical case findings (including haemogram and serum biochemistry), gross and microscopic lesions and virus detection in three HPAI H5N1-infected domestic cats from the USA in 2023. All three cats presented with neurological abnormalities and were euthanized due to a poor prognosis within 2 days (two cats) or 10 days (one cat) of known clinical disease onset. Necropsy consistently revealed pulmonary congestion and oedema, and cerebrocortical malacia with haemorrhage was also seen …

Long-Term Monitoring Of Clodronate In Equine Hair Using Liquid Chromatography-Tandem Mass Spectrometry, Heather K. Knych, D. S. Mckemie, S. Yim, S. D. Stanley, R. M. Arthur

UK CARES Faculty Publications

Given the potential for long-term inhibition of bone remodeling/healing and detrimental effects to horses in training, bisphosphonates are tightly regulated in horseracing. Hair has proven to be an effective matrix for detection of drug administration to horses and has been particularly effective in detecting drugs for a long period of time post administration. Thus, hair may prove to be a useful matrix for detection of administration of this class of drugs. The objective of the current study was to develop an assay and assess the usefulness of hair as a matrix for long-term detection of clodronate to horses. Seven horses …

Growing South Dakota (Summer 2023), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

3 SDSU Little International Celebrates 100 years of Tradition
7 2023 CAFES Celebration of Faculty Excellence
11 South Dakota Agricultural Experiment Station Locations and Research Initiatives
15 Every Acre Counts
16 Undergraduate Research
19 Tanner Sloan: South Dakota State Wrestler & Animal Science Student Takes Home NCAA Division I & U23 World Silver Medals
21 2023 CAFES Outstanding Seniors
23 Robert Streeter: International Advocate for Wildlife Conservation
25 Collegiate Cattlemen's Club
27 Jim and Melody Mielke: Lifelong Donors Contribute to Agriculture in More Ways than One
29 Jackrabbits Now and Then

Molecular Simulation Of B-Cell Epitope Mapping From Nipah Virus Attachment Protein To Construct Peptide-Based Vaccine Candidate: A Reverse Vaccinology Approach, Viol Dhea Kharisma, Farida Aryani Dian, Pavel Burkov, Pavel Scherbakov, Marina Derkho, Ekaterina Sepiashvili, Teguh Hari Sucipto, Arli Aditya Parikesit, Ahmad Affan Ali Murtadlo, Vikash Jakhmola, Rahadian Zainul

Makara Journal of Science

There are no specific drugs or vaccines for Nipah virus (NiV), which is a new Paramyxovirus that infects swine and humans. This study was conducted to investigate B-cell epitope mapping of the NiV attachment glycoprotein and to construct peptide-based vaccine candidates using the reverse vaccinology approach. To generate the linear B-cell epitope, the NiV isolates were extractad from GenBank, NCBI, using the IEDB web server; peptide modeling was conducted using PEP-FOLD3; docking was conducted using PatchDock and FireDock; and in silico cloning was designed using SnapGene. Various peptides were successfully identified from the NiV attachment glycoprotein based on B-cell epitope …

Quantitative Risk Assessment Of Oocyst Versus Bradyzoite Foodborne Transmission Of Toxoplasma Gondii In Brazil, Sophie Zhu, Elizabeth Vanwormer, Beatriz Martínez-López, Lílian Maria Garcia Bahia-Oliveira, Renato Augusto Damatta, Pedro Souto Rodrigues, Karen Shapiro

School of Veterinary and Biomedical Sciences: Faculty Publications

Toxoplasma gondii is a globally distributed zoonotic protozoan parasite. Infection with T. gondii can cause congenital toxoplasmosis in developing fetuses and acute outbreaks in the general population, and the disease burden is especially high in South America. Prior studies found that the environmental stage of T. gondii, oocysts, is an important source of infection in Brazil; however, no studies have quantified this risk relative to other parasite stages. We developed a Bayesian quantitative risk assessment (QRA) to estimate the relative attribution of the two primary parasite stages (bradyzoite and oocyst) that can be transmitted in foods to people in …