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Articles 5971 - 6000 of 9758
Full-Text Articles in Veterinary Medicine
Premature Expression Of The Latency-Related Rna Encoded By Bovine Herpesvirus Type 1 Correlates With Higher Levels Of Beta Interferon Rna Expression In Productively Infected Cells, Sandra Perez, Florencia Meyer, Kazima Saira, Alan R. Doster, Clinton J. Jones
Premature Expression Of The Latency-Related Rna Encoded By Bovine Herpesvirus Type 1 Correlates With Higher Levels Of Beta Interferon Rna Expression In Productively Infected Cells, Sandra Perez, Florencia Meyer, Kazima Saira, Alan R. Doster, Clinton J. Jones
School of Veterinary and Biomedical Sciences: Faculty Publications
Bovine herpesvirus type 1 (BHV-1) is an important pathogen that can initiate bovine respiratory disease complex. Like other members of the subfamily Alphaherpesvirinae, BHV-1 establishes latency in sensory neurons. The latency-related (LR) gene expresses a family of alternatively spliced transcripts in infected sensory neurons that have the potential to encode several LR proteins. An LR mutant virus that contains three stop codons near the 5’ terminus of the first open reading frame in the LR gene does not express two LR proteins or reactivate from latency. In addition, the LR mutant virus induces higher levels of apoptosis in trigeminal …
Effects Of Fescue Herbicides Plateau® And Cimarron® On Pregnancy Maintenance In Broodmares And On Alkaloid Concentrations In Endophyte Infected Tall Fescue, Kathleen Scarlett Black
Effects Of Fescue Herbicides Plateau® And Cimarron® On Pregnancy Maintenance In Broodmares And On Alkaloid Concentrations In Endophyte Infected Tall Fescue, Kathleen Scarlett Black
University of Kentucky Master's Theses
Ingestion of endophyte infected (E+) fescue by pregnant mares can cause significant reproductive problems. Plateau® and Cimarron® herbicides suppress fescue while leaving desired forages unharmed. To determine if these herbicides are harmful to pregnant mares, they were allowed to graze pastures treated with Plateau®, Cimarron®, or vehicle carrier. Pregnancies were monitored via ultrasonography, blood chemistry, and hematology. Of the components measured only creatinine differed among treatments over time (P=0.0003) and that increase was only significant in one of four studies.
Two additional experiments were conducted to determine the effect of the herbicides on alkaloids within E+ fescue. A greenhouse experiment …
Advancing Epidemiological Science Through Computational Modeling: A Review With Novel Examples, Scott M. Duke-Sylvester, Eli N. Perencevich, Jon P. Furuno, Leslie A. Real, Holly Gaff
Advancing Epidemiological Science Through Computational Modeling: A Review With Novel Examples, Scott M. Duke-Sylvester, Eli N. Perencevich, Jon P. Furuno, Leslie A. Real, Holly Gaff
Biological Sciences Faculty Publications
Computational models have been successfully applied to a wide variety of research areas including infectious disease epidemiology. Especially for questions that are difficult to examine in other ways, computational models have been used to extend the range of epidemiological issues that can be addressed, advance theoretical understanding of disease processes and help identify specific intervention strategies. We explore each of these contributions to epidemiology research through discussion and examples. We also describe in detail models for raccoon rabies and methicillin-resis-tant Staphylococcus aureus, drawn from our own research, to further illustrate the role of computation in epidemiological modeling.
The Influence Of Veterinary Schools On The Veterinary Labor Market, Kyle Bosh
The Influence Of Veterinary Schools On The Veterinary Labor Market, Kyle Bosh
MPA/MPP/MPFM Capstone Projects
Problem Statement:
Does the presence of a veterinary school in a state influence the supply of veterinarians practicing in that state? Citizens and animals in states without a veterinary school are at a disadvantage if the presence of a veterinary school significantly improves public health and access to medical services in those states that have a veterinary school.
Research Strategy:
The unit of analysis in this study is 50 states and the District of Columbia. The study is designed as a cross-sectional analysis of 2006 pet, 2002 livestock, 2006 human, labor, and economic data. The study uses simultaneous equations to …
Small-Ruminant Lentivirus Enhances PrpSc Accumulation In Cultured Sheep Microglial Cells, James B. Stanton, Donald P. Knowles, Katherine I. O'Rourke, Lynn M. Herrmann-Hoesing, Bruce A. Mathison, Timothy V. Baszler
Small-Ruminant Lentivirus Enhances PrpSc Accumulation In Cultured Sheep Microglial Cells, James B. Stanton, Donald P. Knowles, Katherine I. O'Rourke, Lynn M. Herrmann-Hoesing, Bruce A. Mathison, Timothy V. Baszler
Zoonotics and Wildlife Disease: Publications
Sheep scrapie is the prototypical transmissible spongiform encephalopathy (prion disease), which has a fundamental pathogenesis involving conversion of normal cellular prion protein (PrPC [C superscript stands for cellular]) to disease-associated prion protein (PrPSc [Sc superscript stands for sheep scrapie]). Sheep microglial cell cultures, derived from a prnp 136VV/171QQ near-term fetal brain, were developed to study sheep scrapie in the natural host and to investigate potential cofactors in the prion conversion process. Two culture systems, a primary cell culture and a cell line transformed with the large T antigen of simian virus 40, were developed, and both were identified …
Bovine Herpesvirus Type 1 Induces Cell Death By A Cell Type Dependent Fashion, Vicki Geiser, Suzanne Rose, Clinton J. Jones
Bovine Herpesvirus Type 1 Induces Cell Death By A Cell Type Dependent Fashion, Vicki Geiser, Suzanne Rose, Clinton J. Jones
School of Veterinary and Biomedical Sciences: Faculty Publications
Bovine herpesvirus 1 (BHV-1), a member of the alpha-herpesvirinae subfamily, causes significant losses to the cattle industry. BHV-1 establishes latency in trigeminal ganglionic sensory neurons, but periodically reactivates from latency. Previous studies suggested that infection with BHV-1 induced novel morphological changes in rabbit skin cells (RS) versus bovine kidney cells (MDBK). Consequently, we hypothesized that viral infection led to a novel form of cell death in RS cells compared to MDBK cells. To test this hypothesis, we examined the levels of apoptosis in these cell types following infection with BHV-1. Infection of RS, but not MDBK, cells leads to high …
A Review Of The Biology Of Bovine Herpesvirus Type 1 (Bhv-I), Its Role As A Cofactor In The Bovine Respiratory Disease Complex And Development Of Improved Vaccines, Clinton J. Jones, Shafiqul Chowdhury
A Review Of The Biology Of Bovine Herpesvirus Type 1 (Bhv-I), Its Role As A Cofactor In The Bovine Respiratory Disease Complex And Development Of Improved Vaccines, Clinton J. Jones, Shafiqul Chowdhury
School of Veterinary and Biomedical Sciences: Faculty Publications
Infection of cattle by bovine herpesvirus type 1 (BHV-1) can lead to upper respiratory tract disorders, conjunctivitis, genital disorders and immune suppression. BHV-1-induced immune suppression initiates bovine respiratory disease complex (BRDC), which costs the US cattle industry approximately 3 billion dollars annually. BHV-1 encodes at least three proteins that can inhibit specific arms of the immune system: (i) bICPO inhibits interferon-dependent transcription, (ii) the UL41.5 protein inhibits CD8+ T-cell recognition of infected cells by preventing trafficking of viral peptides to the surface of the cells and (iii) glycoprotein G is a chemokine-binding protein that prevents homing of lymphocytes to …
How Does Age Affect Baseline Screening Mammography Performance Measures? A Decision Model, James Keen, John D. Keen
How Does Age Affect Baseline Screening Mammography Performance Measures? A Decision Model, James Keen, John D. Keen
School of Veterinary and Biomedical Sciences: Faculty Publications
Background: In order to promote consumer-oriented informed medical decision-making regarding screening mammography, we created a decision model to predict the age dependence of the cancer detection rate, the recall rate and the secondary performance measures (positive predictive values, total intervention rate, and positive biopsy fraction) for a baseline mammogram.
Methods: We constructed a decision tree to model the possible outcomes of a baseline screening mammogram in women ages 35 to 65. We compared the single baseline screening mammogram decision with the no screening alternative. We used the Surveillance Epidemiology and End Results national cancer database as the primary input to …
Characterization And Comparison Of Cell Frequency, Growth, And Multipotential Differentiation Of Adult Mesenchymal Stromal Cells Derived From Equine Bone Marrow And Adipose Tissue, Martin Andreas Vidal
Characterization And Comparison Of Cell Frequency, Growth, And Multipotential Differentiation Of Adult Mesenchymal Stromal Cells Derived From Equine Bone Marrow And Adipose Tissue, Martin Andreas Vidal
LSU Doctoral Dissertations
Equine bone marrow-derived mesenchymal stromal cells (MSCs) and adipose tissue-derived stromal cells (ASCs) were compared for frequency within their respective tissues, cell doubling characteristics and differentiation multipotential in culture based on histochemical staining and compositional analysis. Equine MSCs and ASCs from young adult horses were harvested and isolated from sternal bone marrow and supragluteal subcutaneous adipose tissue, respectively, and grown up to passage 10 (P10) to determine cell doubling characteristics. Limit dilution assays were performed on primary and passaged (P2, P4) MSCs and ASCs to determine the frequency of colony forming units with a fibroblastic phenotype (CFU-F), and the frequency …
Determination Of The Amino Acid And Chemical Composition Of Canned Smoked Mussels (Mytilus Galloprovincialis, L.), Gülgün F. Ünal Şengör, Hüseyi̇n Gün, Hani̇fe Kalafatoğlu
Determination Of The Amino Acid And Chemical Composition Of Canned Smoked Mussels (Mytilus Galloprovincialis, L.), Gülgün F. Ünal Şengör, Hüseyi̇n Gün, Hani̇fe Kalafatoğlu
Turkish Journal of Veterinary & Animal Sciences
In this research smoking and canning techniques were applied to cultured mussels (Mytilus galloprovincialis, L.) from the Çanakkkale Strait in Eceabat, Turkey. Mussels that were smoked by liquid and traditional methods were canned in different sauces. The chemical composition and amino acid composition of the canned smoked mussels were determined by the results of laboratory analyses. As a result of smoking and canning mussels, a food with high nutritional value was obtained. It was determined that the heat applied to the product during smoking and canning did not effect amino acid composition negatively and that if any losses occur, they …
Effect Of Different Diets On Growth Performance And Feed Efficiency In Early Weaned Piglets, Fi̇li̇z Akdağ, Özkan Elmaz, Can Kutay, Hidir Demi̇r
Effect Of Different Diets On Growth Performance And Feed Efficiency In Early Weaned Piglets, Fi̇li̇z Akdağ, Özkan Elmaz, Can Kutay, Hidir Demi̇r
Turkish Journal of Veterinary & Animal Sciences
In this study the effects of different diets on the survival, growth performance, and feed efficiency of early weaned piglets were investigated. In this study, 96 crossbred piglets obtained from uncontrolled interbreeding of Bulgarian Large White, Large Black, and Turopolje for many years, which had weaned at the age of 28 days, were used and they were grouped equally to form one control and 2 experimental groups. Each group was fed with a different protein diet for 4 weeks: the first group was fed sunflower meal (SFM), the second group was fed soybean meal (SBM), and the control group was …
A Study Of Somatic Cell Counts In The Milk Of Holstein-Friesian Cows Managed In Mediterranean Climatic Conditions, Atakan Koç
Turkish Journal of Veterinary & Animal Sciences
Somatic cell counts (SCC) in the milk of Holstein-Friesian (HF) cows were determined by direct microscopic SCC technique. For a two-year period, milk samples were collected monthly from buckets during the morning and evening milkings at 4 different dairy farms. In total, 1,464 SCC readings from 88 HF cows were analyzed by using repeated measures. Herd, lactation month, parity, milking time, and herd interaction effects on SCC were statistically significant (P < 0.05). The average SCC in milk per herd was between 296,483 and 688,811 cells/ml. SCC in milk increased as parity increased. The average SCC in milk from evening milking was about 83,165 cells/ml higher than from morning milking. Improving milking management, reducing stress, providing extra care during the first month of lactation, and milking at a uniform interval will help to decrease SCC in milk and the prevalence of mastitis.
Semen Collection From Japanese Quail (Coturnix Japonica) Using A Teaser Female, Bronislawa Chelmonska, Anna Jerysz, Ewa Lukaszewicz, Artur Kowalczyk, Irek Malecki
Semen Collection From Japanese Quail (Coturnix Japonica) Using A Teaser Female, Bronislawa Chelmonska, Anna Jerysz, Ewa Lukaszewicz, Artur Kowalczyk, Irek Malecki
Turkish Journal of Veterinary & Animal Sciences
The quantity and quality of Japanese quail (Coturnix japonica) semen collected individually after stimulation by a teaser female was assessed. From among 25 mature males, 10 were selected for experimental purposes on the basis of reaction intensity to stimulation, size of the proctodeal foam gland, and ejaculation speed. Males were kept individually in cages (32 x 44 x 24 cm) and 5 females that served for male stimulation were kept in a group cage (60 x 45 x 48 cm). The applied method of semen collection was very effective and with it we obtained ejaculates of good quality; average volume …
Harvesting And Evaluation Of Riverine Buffalo Follicular Oocytes, Huma Jamil, Hafiz Abdus Samad, Zafar Iqbal Qureshi, Najeeb Ur Rehman, Laeed Akbar Lodhi
Harvesting And Evaluation Of Riverine Buffalo Follicular Oocytes, Huma Jamil, Hafiz Abdus Samad, Zafar Iqbal Qureshi, Najeeb Ur Rehman, Laeed Akbar Lodhi
Turkish Journal of Veterinary & Animal Sciences
The low superovulatory response and poor recovery rate of oocytes are major impediments to the application of in vitro fertilization and embryo transfer technology in buffaloes. The present study aimed to evaluate the comparative efficacy of oocyte collection methods, season (low and peak breeding season), and ovarian status (presence or absence of corpus luteum) on the recovery rate and quality of recovered buffalo follicular oocytes. For this purpose riverine buffalo ovaries were collected from buffaloes slaughtered at Faisalabad Municipal Corporation Slaughter House. To study the efficiency of 3 different recovery methods the ovaries were dissected (n = 291), punctured (n …
The Effect Of Stocking Density On Stress Reaction In Broiler Chickens During Summer, Mehmet Kenan Türkyilmaz
The Effect Of Stocking Density On Stress Reaction In Broiler Chickens During Summer, Mehmet Kenan Türkyilmaz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the effect of stocking density on the heterophil to lymphocyte ratio (H:L), blood corticosterone concentration, immune response, and some performance parameters in broilers during summer. In all, 300 1-day-old commercial broiler chicks (Ross 308) were housed at densities of 15, 20, and 25 birds/m^2, with 2 replicates of each. H:L ratio and blood corticosterone concentration were used to measure the level of stress in the birds. Newcastle disease (ND) antibody titers were also analyzed to determine the level of immune response with a commercial ELISA kit. Mean H:L ratio, corticosterone concentration, and …
Antibiotic Susceptibility Patterns Of Pseudomonas Aeruginosa Strains Isolated From Dogs With Otitis Externa, Süheyla Türkyilmaz
Antibiotic Susceptibility Patterns Of Pseudomonas Aeruginosa Strains Isolated From Dogs With Otitis Externa, Süheyla Türkyilmaz
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were to isolate Pseudomonas aeruginosa from dogs with otitis externa (OE), to determine the susceptibility of isolated strains to antibiotics, and to evaluate the incidence of the infection in the Aydın region. For this purpose, ear swab samples were obtained from 92 dogs with symptoms of OE infection and the samples were examined bacteriologically. Bacteria were isolated from 88 (96%) of 92 samples but no isolation was performed on 4 (4%) dogs. In total 93 microorganisms were identified. Among these microorganisms, staphylococci species were the most isolated one in number (43%), followed by P. aeruginosa …
Mannan Oligosaccharides (Mos) From Saccharomyces Cerevisiae In Broilers: Effects On Performance And Blood Biochemistry, İlkay Yalçinkaya, Tüli̇n Güngör, Mehmet Başalan, Evren Erdem
Mannan Oligosaccharides (Mos) From Saccharomyces Cerevisiae In Broilers: Effects On Performance And Blood Biochemistry, İlkay Yalçinkaya, Tüli̇n Güngör, Mehmet Başalan, Evren Erdem
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to investigate the effects of mannan oligosaccharides (MOSs), which are commonly used as alternatives to antibiotics, on the growth performance and some blood parameters in broiler chickens. The study included 120 Ross 308 male broiler chicks (1-day-old at the beginning of the study). There were 4 treatment groups, each consisting of 3 replicates. MOSs were added at the level of 0.05%, 0.10%, and 0.15% to the starter and finisher diets. The experiment lasted 42 days. Body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) were not significantly different (P > 0.05) between MOS treatment …
The Determination Of The Shelf Life And Some Nutritional Components Of Gilthead Seabream (Sparus Aurata L., 1758) After Cold And Hot Smoking, Şengül Bi̇lgi̇n, Mustafa Ünlüsayin, Levent İzci̇, Ali̇ Günlü
The Determination Of The Shelf Life And Some Nutritional Components Of Gilthead Seabream (Sparus Aurata L., 1758) After Cold And Hot Smoking, Şengül Bi̇lgi̇n, Mustafa Ünlüsayin, Levent İzci̇, Ali̇ Günlü
Turkish Journal of Veterinary & Animal Sciences
This study aimed to determine the shelf life, as well as chemical and microbiological quality of gilthead seabream (Sparus aurata L., 1758) prepared by 2 different methods of smoking (hot smoking and cold smoking). The effects of hot and cold smoking on the chemical composition and microbial load of gilthead seabream, as well as organoleptic analysis of the smoked product were investigated. Significant (P < 0.05) differences were found in the chemical composition of fresh and smoked seabream. The panelists liked the hot smoked fish more than the cold smoked fish, according to sensory analysis results. Changes in pH, thiobarbituric acid (TBA), and total volatile basic nitrogen (TVB-N) values were significant (P < 0.05) during storage at 4 °C. Microbiological analysis results demonstrated that the smoking techniques reduced the microbial content of the fish, whereas microbial content increased during storage. The smoking methods tested had a small effect on the level of vitamin D_3 in gilthead seabream.
Epidemiology Of Tropical Theileriosis In The Cappadocia Region, Abdullah İnci̇, Anil İça, Alparslan Yildirim, Zati̇ Vatansever, Ayşe Çakmak, Hasan Albasan, Yücel Çam, Ayhan Atasever, Önder Düzlü
Epidemiology Of Tropical Theileriosis In The Cappadocia Region, Abdullah İnci̇, Anil İça, Alparslan Yildirim, Zati̇ Vatansever, Ayşe Çakmak, Hasan Albasan, Yücel Çam, Ayhan Atasever, Önder Düzlü
Turkish Journal of Veterinary & Animal Sciences
A total of 554 cattle from the Cappadocia region were divided into 5 different groups according to vaccination and were examined for Theileria annulata by microscope and indirect fluorescence antibody test (IFAT). The sampled cattle were checked for tick infestation. The prevalence of T. annulata was 60.5% by microscopic examination and 67.5% by IFAT. According to the microscopic examination, the prevalence of T. annulata was significantly (P < 0.001) higher in the unvaccinated group. The seropositivity according to IFAT was significantly (P < 0.001) lower in the unvaccinated group. The morbidity was significantly (P < 0.001) higher in the unvaccinated, semi-grazed, pure breed, and >2 years age groups. The mortality was significantly higher in the unvaccinated (P < 0.001), semi-grazed (P < 0.05), pure breed (P < 0.05), and female (P < 0.001) groups. A total of 1585 tick specimens belonging to the genera Hyalomma, Rhipicephalus, Dermacentor, Boophilus, and Ornithodorus were detected in 21.48% of examined cattle. The developing forms of T. annulata were determined in the H. anatolicum anatolicum ticks microscopically. Out of 554 cattle, 156 (27.61%) were diagnosed with acute tropical theileriosis. Out of these 156 cattle, 86 (56.21%) died due to the disease.
Uterine And Ovarian Metastasis Of Transmissible Venereal Tumor In A Bitch, Ayhan Baştan, Duygu Baki̇ Acar, Mehmet Cengi̇z
Uterine And Ovarian Metastasis Of Transmissible Venereal Tumor In A Bitch, Ayhan Baştan, Duygu Baki̇ Acar, Mehmet Cengi̇z
Turkish Journal of Veterinary & Animal Sciences
In this case report, metastasis of a transmissible venereal tumor (TVT) to the uterus and ovaries is presented. A 7-year-old female boxer dog with hemorrhagic vulvar discharge and a vaginal mass was brought to Ankara University Faculty of Veterinary Medicine, Clinics of Gynecology and Obstetrics. TVT was diagnosed by clinical findings and cytologic examination. Multifocal nodules in the uterus and enlargements of the uterine wall were detected by ultrasonographic examination. Ovariohysterectomy was performed because of uterine pathology. Histopathologic examination of the uterus and ovarium showed that the multifocal masses in the uterus and ovaries originated from vaginal TVT.
Influence Of Additives And Fermentation Periods On Silage Characteristics, Chemical Composition, And In Situ Digestion Kinetics Of Jambo Silage And Its Fodder In Nili Buffalo Bulls, M. Aasif Shahzad, Muhammad Sarwar, Mahr-Un Nisa, N. A. Tauqir
Influence Of Additives And Fermentation Periods On Silage Characteristics, Chemical Composition, And In Situ Digestion Kinetics Of Jambo Silage And Its Fodder In Nili Buffalo Bulls, M. Aasif Shahzad, Muhammad Sarwar, Mahr-Un Nisa, N. A. Tauqir
Turkish Journal of Veterinary & Animal Sciences
Jambo grass (Sorghum bicolour x Sorghum sudanefe) harvested 50 days after sowing was ensiled in laboratory silos using 2 additives, cane molasses and ground corn grains, each at the rate of 2%, 4%, and 6% levels for 30, 35, and 40 days at room temperature (28 °C). Data regarding incubation days were pooled. The pH and lactic acid concentration in jambo grass silage were not affected by the additive type, level, or fermentation period. Dry matter (DM) content of jambo silage was significantly (P < 0.05) affected by additive type; however, additive levels and fermentation periods did not affect its DM contents. Crude protein content of jambo grass was not affected by ensiling time, additive type, or level. The true protein contents of jambo grass silage were significantly (P < 0.05) higher with molasses than with ground corn. Neutral detergent fiber (NDF) and hemicellulose content of jambo grass were significantly (P < 0.05) affected by additives and their levels, but fermentation periods did not influence the NDF contents of jambo grass silage. Cellulose, acid detergent fiber, and acid detergent lignin contents of jambo silage were not affected by additive type, level, or fermentation period. Therefore, jambo grass ensiled with 2% molasses for 30 days was selected for in situ digestion kinetics in bulls. Dry matter and NDF degradabilities of jambo grass were significantly higher than those of its silage at 48 h of ruminal incubation. Ruminal lag time, and rate and extent of DM and NDF degradation were similar for jambo grass and its silage. The results of this study implied that jambo grass ensiled with 2% molasses for 30 days has similar nutritive value to that of its forage in bulls.
Application Of Mentofin^® In Broilers With Clinical Infectious Bursal Disease To Reduce Escherichia Coli Related Problems After Vaccination Against Newcastle Disease, K. Tayfun Çarli, Kaan Önat, Elçi̇n Günaydin
Application Of Mentofin^® In Broilers With Clinical Infectious Bursal Disease To Reduce Escherichia Coli Related Problems After Vaccination Against Newcastle Disease, K. Tayfun Çarli, Kaan Önat, Elçi̇n Günaydin
Turkish Journal of Veterinary & Animal Sciences
In this study Mentofin^® was used in commercial broiler chickens naturally exposed to infectious bursal disease virus (IBDV) and administered live Newcastle disease virus (NDV) vaccine to evaluate the ability of Mentofin^® to reduce Escherichia coli-related respiratory lesions, immunomodulate Newcastle disease (ND) vaccine response, change pharyngeal aerobic bacterial counts, and have certain impacts on specific production parameters. Mentofin^® was added to the drinking water (100 ml/500 l water) of broiler chickens aged between 3 and 5 days. ND and IBD vaccinations were administered via drinking water to 15-day-old chickens. NDV vaccination was given again when the chickens were 20 days …
Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan
Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan
Turkish Journal of Veterinary & Animal Sciences
Presence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks (16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples …
A Bicistronic Dna Vaccine Containing Gene Of Fmdv And Bovine Ifn-\Alpha Can Prime Humoral And Cellular Immune Responses Of Guinea Pigs, Huichen Guo, Shiqi Sun, Jiangtao Ma, Zaixin Liu, Xiangtao Liu, Qingge Xie
A Bicistronic Dna Vaccine Containing Gene Of Fmdv And Bovine Ifn-\Alpha Can Prime Humoral And Cellular Immune Responses Of Guinea Pigs, Huichen Guo, Shiqi Sun, Jiangtao Ma, Zaixin Liu, Xiangtao Liu, Qingge Xie
Turkish Journal of Veterinary & Animal Sciences
The study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine against foot-and-mouth disease virus (FMDV). Genes encoding the P1, 2A, 3C of FMDV O/China99 and bovine IFN-\alpha were cloned into pcDNA3.1 (+) expression vector under CMV promoter and FMDV IRES control, respectively. The recombinant plasmids were administered to guinea pigs by intramuscular injection with mono- or bicistronic expression plasmids and aurintricarboxylic acid (ATA). After 2 sequential vaccinations with plasmid DNA, all of guinea pigs were challenged with 100 ID_{50} FMDV O/China99. Anti-FMDV antibodies were detected by ELISA and neutralization assay, and the phenotyping of T-cell …
Comparative Results Of Survival Of Vitrified Biopsied Goat Embryos And Mouse Morulae, Aqin Chen, Rui Zhang, Songdong Yu
Comparative Results Of Survival Of Vitrified Biopsied Goat Embryos And Mouse Morulae, Aqin Chen, Rui Zhang, Songdong Yu
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to find an efficient cryoprotectant and a suitable procedure for vitrification of mouse morulae. The method found with mouse morulae was then applied to the cryopreservation of biopsied goat embryos. Mouse morulae were exposed, in 1- or 2-step, to the ethylene glycol (EG)-based solutions EFS30 or EDFS30, which contained 30% EG and 15% EG plus 15% dimethylsulphoxide (DMSO), respectively, in phosphate buffer saline (PBS) containing 30% (w/v) Ficoll and 0.5 M sucrose, cooled in liquid nitrogen and warmed up rapidly. After warming, the rate of the hatched blastocyst formation in mouse morulae vitrified with …
The Effects Of A Nutritionally Unbalanced Diet On Some Blood And Postpartum Fertility Parameters In Dairy Cows, Erol Alaçam, Şaki̇r Doğan Tuncer, M. Rifat Salmanoğlu, Seher Küçükersan, Kemal Küçükersan, Abdurrahman Özlüer
The Effects Of A Nutritionally Unbalanced Diet On Some Blood And Postpartum Fertility Parameters In Dairy Cows, Erol Alaçam, Şaki̇r Doğan Tuncer, M. Rifat Salmanoğlu, Seher Küçükersan, Kemal Küçükersan, Abdurrahman Özlüer
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine the effects of mineral imbalances and negative energy balance on reproductive functions during the early and later stages of the postpartum period in dairy cows. The study included 100 randomly selected multiparous Holstein cows aged between 2 and 8 years (mean weight: 650 kg). The animals´ diets were not manipulated and they were fed the rations used by the study farm. Blood samples were taken on postpartum days 0, 20, 24, 30, 31, 38, 45, 60, and 90. Cows with calving and/or puerperium problems were recorded. Analyses were made for non-esterified fatty acids, …
Genetic Heterogeneity And Antibiotic Susceptibility Of Vibrio Alginolyticus Strains Isolated From Horse-Mackerel (Trachurus Trachurus L., 1758), Selmi̇n Özer, Gönül Aslan, Seda Tezcan, Pinar Sevi̇m Bulduklu, Mehmet Sami̇ Seri̇n, Gürol Emekdaş
Genetic Heterogeneity And Antibiotic Susceptibility Of Vibrio Alginolyticus Strains Isolated From Horse-Mackerel (Trachurus Trachurus L., 1758), Selmi̇n Özer, Gönül Aslan, Seda Tezcan, Pinar Sevi̇m Bulduklu, Mehmet Sami̇ Seri̇n, Gürol Emekdaş
Turkish Journal of Veterinary & Animal Sciences
This study was performed to investigate the presence of halophylic Vibrio spp. in horse-mackerel (Trachurus trachurus L., 1758). In total, 80 fish samples were collected from fish markets and supermarkets between 04.04.2006 and 16.05.2006 in the city of Mersin. All fish samples were microbiologically tested for halophylic Vibrio spp. Nine (11.25%) Vibrio spp. strains were isolated and identified as V. alginolyticus. Molecular epidemiological discrimination and antimicrobial susceptibility patterns of V. alginolyticus strains were also analysed by arbitrarily primed polymerase chain reaction (AP-PCR) and Kirby-Bauer disc diffusion, respectively. Genotypic heterogeneity of these 9 isolates discriminated by AP-PCR determined that while only …
Effects Of Dietary Boric Acid Supplementation On Performance, Eggshell Quality And Some Serum Parameters In Aged Laying Hens, Derya Yeşi̇lbağ, Mustafa Eren
Effects Of Dietary Boric Acid Supplementation On Performance, Eggshell Quality And Some Serum Parameters In Aged Laying Hens, Derya Yeşi̇lbağ, Mustafa Eren
Turkish Journal of Veterinary & Animal Sciences
The effects of dietary boric acid (BA) supplementation on feed consumption, feed efficiency, egg production, egg weight, eggshell thickness, eggshell breaking strength, and some serum parameters (Ca, P, and Mg) were examined in 100 layer hens at 60 weeks of age. Commercial corn-soy-based layer diets were formulated as basal diets for the control treatment. Basal diets were supplemented with 25, 50, and 100 mg/kg BA (H_3BO_3), respectively, in experimental groups I, II, and III. In total, 4 dietary treatments were employed in the experiment. The experiment lasted 75 days. Feed efficiency and egg production values were not affected significantly by …
Investigation Of The Incidence And Diagnosis Of Subclinical Mastitis In Early Lactation Period Cows, Ayhan Baştan, Ci̇han Kaçar, Duygu B. Acar, Mi̇that Şahi̇n, Mehmet Cengi̇z
Investigation Of The Incidence And Diagnosis Of Subclinical Mastitis In Early Lactation Period Cows, Ayhan Baştan, Ci̇han Kaçar, Duygu B. Acar, Mi̇that Şahi̇n, Mehmet Cengi̇z
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to compare the results of the California mastitis test (CMT) and bacteriological culture for detection of subclinical intramammary infections after 10 days postpartum. Samples were collected from 102 cows. The CMT was performed once on each cow. The results of bacteriological culture and the CMT were compared in 344 milk samples. Two hundred fifty samples were CMT (+) and 94 samples were CMT (-); 224 samples were bacteriologic culture (+) and 120 samples were culture (-). Both CMT and culture (+) samples numbered 212, CMT and culture (-) samples 82, CMT (+) and culture …
Antibioresistance Of Escherichia Coli Strains Isolated From Chicken Colibacillosis In Western Algeria, Abdelhamid Hamoudi, Hebib Aggad
Antibioresistance Of Escherichia Coli Strains Isolated From Chicken Colibacillosis In Western Algeria, Abdelhamid Hamoudi, Hebib Aggad
Turkish Journal of Veterinary & Animal Sciences
In western Algeria, 251 Escherichia coli isolates were recovered from broilers with clinical signs and lesions of colibacillosis. Serotyping showed that 82% of the isolates belong to one of the following serotypes: O78, O2, and O1. Antibiograms revealed a high level of resistance to oxytetracycline (82%), amoxicilline and ampicilline (47%), trimethoprim-sulfmethoxazole (42%), oxolinic acid and flumequine (31%). However, only a low percentage of strains were resistant to enrofloxacine (6%) and colistine (3%). Although multiresistance was common, low association was found between the serotype and antibioresistance.