Veterinary Medicine Commons^™

Alteration Of Gene Expression In Microvascular Endothelial Cells After Exposure To Foot-And-Mouth Disease Virus 146s Antigen, Tao Zhang, Shuang Zhang, Li-Fang Tian, Ge Hu, Zhan-Wei Suo, Wu Chen, Hui-Qin Duan, Xiang Mu, Ke-Dao Teng

Turkish Journal of Veterinary & Animal Sciences

To investigate the global response of microvascular endothelial cells (MVECs) to foot-and-mouth (FMDV)disease vaccine (FMDV) antigen and to explore the role of MVECs in FMD vaccine immunity, the gene expression alterations of rat myocardium microvascular endothelial cells (RMMVECs) after stimulation with FMD virus (FMDV) 146S antigen were assayed using cDNA microarrays. We observed that 18 genes and 67 genes in RMMVECs were respectively altered by FMDV 146S antigen at 6 h and 24 h, respectively. The former were all up-regulated genes, and the latter included 60 up-regulated genes and 7 down-regulated genes. Among the differentially expressed genes, many were associated …

Diet Preference And Breeding Success In Captive-Bred Greater Rheas (Rhea Americana): A Preliminary Study, Gisela Bazzano, Joaquin. L. Navarro, Monica. B. Martella

Turkish Journal of Veterinary & Animal Sciences

We conducted 2 consecutive experiments to verify whether adult captive Greater rheas (Rhea americana) could select diets differing in nutritional and energetic content, and to evaluate the effect of those diets on egg production and hatchability. From August to January, animals were offered four diets: processed feed for rheas (diet 1), diet 1 + soybean (diet 2), processed feed for chicken (diet 3), and diet 3 + soybean (diet 4). Rheas preferred the diets containing feed for chicken (diets 3 and 4, 179,67 ± 12.12 [S.E.] g/ind./day). Diet 2 was more consumed (95.67 ± 16.70 g/ind./day) than diet 1 (60.09 …

Association Between Polymorphisms Of Cyp19, Cyp21, And Er1 Genes And Milk Production Traits In Black-And-White Cattle, Magdalena Jedrzejczak, Wilhelm Grzesiak, Iwona Szatkowska, Andrzej Dybus, Magdalena Muszynska, Daniel Zaborski

Turkish Journal of Veterinary & Animal Sciences

The relationships between the SNPs of the cytochrome P450 gene (CYP19/PvuII), steroid 21-hydroxylase (P450c21) gene (CYP21/HpaII), 2 polymorphic sites of estrogen receptor alpha gene (ER1/BglI and ER1/SnaBI), and milk production traits of Black-and-White cattle were analyzed. A total of 472 cows were included in the study and genotyped using PCR-RFLP. The frequencies of alleles for the Black-and-White cows were as follows: 0.923 - CYP19A, 0.077 - CYP19B, 0.042 - ER1/BglIA, and 0.958 for ER1/BglIG. For ER1/SnaBI, the frequency of allele A was 0.960 and that of allele G was 0.040. In the CYP21, all cows were genotyped as AA (no …

Haematology Of Downer Dairy Cows With Fatty Liver, Emmanouil Kalaitzakis, Nikolaos Panousis, Nikolaos Roubies, Eleni Kaldrymidoy, Harilaos Karatzias

Turkish Journal of Veterinary & Animal Sciences

This study examined downer dairy cows with concurrent fatty liver of different degrees and evaluated the haematological values, the relationship and joint occurrence of fatty liver severity and haematology and compared the haematological values in the downer animals that finally died or cured. Blood and liver biopsy samples were obtained from 36 Holstein downer cows shortly after they became recumbent and before they were treated. Blood analysis included determination of haematocrit, haemoglobin, white blood, granular cell and lymphocytes count, and platelet number. Liver tissue was examined histologically and was classified according to severity of fatty liver. The majority of downer …

Aflatoxin M1 Levels In Retail Yoghurt And Ayran In Erzurum In Turkey, Meryem Aydemi̇r Atasever, Mustafa Atasever, Korhan Özturan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the presence and levels of aflatoxin M1 (AFM1) in yoghurt and ayran consumed in the province of Erzurum, Turkey. For this purpose, a total of 80 yoghurt samples and 80 ayran samples were randomly obtained from markets. The samples' AFM1 content and concentrations were examined by competitive ELISA method. AFM1 at detectable level (5 ng/kg) was found in 87.5% of the yoghurt samples and in 90.0% of the ayran samples. AFM1 levels in 16 (20%) yoghurt and in 11 (13.6%) ayran samples exceeded the maximum tolerable limit of the Turkish Food Codex. The …

Association Analysis Of Aquaporin 7 (Aqp7) Gene Variants With Semen Quality And Fertility In Bulls, Tenghe Ma, Jifeng Liu, Ruifeng Zhao, Hao Jiang, Lisheng Dai, Yumin Zhao, Zhihui Zhao, Jiabao Zhang

Turkish Journal of Veterinary & Animal Sciences

Aquaporin 7 (AQP7) gene as a candidate Antifreeze gene was investigated and associated with fresh and frozen semen quality traits in 45 Simmental and Charolais bulls. PCR-SSCP, PCR-RFLP, and sequencing method were employed to detect the single nucleotide polymorphisms (SNPs) of the AQP7 gene. Two mutations, A264G and G371C, were identified. The allele frequencies were A (0.97, 0.96) to B (0.03, 0.04) and C (0.97, 0.91) to D (0.03, 0.09) in Simmental and Charolais bulls, respectively. A264G substitution was significantly associated with acrosome integrity ratio (P < 0.01) and motility (P < 0.05) in frozen semen. Meanwhile, G371C substitution was significantly associated with acrosome integrity ratio (P < 0.05), percentages of viable sperm (P < 0.05), and motility (P < 0.01) in frozen semen. The results prove that AQP7 gene SNPs are useful for marker-assisted selection in early seed selection in the bull industry.

Effects Of Varying Levels Of Protected Fat On Performance Of Shami Goats During Early And Mid Lactation, Hosam Titi

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the responses of early lactating Shami goats to varying amounts of dietary supplementation of Ca salts of fatty acids. Shami goats (n = 75) in their first week of lactation were randomly assigned to 3 isonitrogenous dietary (TMR) treatments containing 0%, 3%, or 5% added Ca salt. The study took 60 days during which the following variables were measured: milk production and composition, body weight, feed intake, serum cholesterol and triglyceride, kids weaning weight, and milk fatty acids. Milk production and energy corrected milk were higher (P < 0.05) at 5% level. Percentages of milk composition were higher (P < 0.05) at 3% with CP% not different from 5%. No differences were observed in yields of different milk components including casein, final body weights, feed intake, or weaning weights of kids. Intake of metabolizable energy was higher (P < 0.05) at 5% level of Ca salts. Differences in milk fatty acids were mainly restricted to unsaturated ones with no effect on the saturated fatty acids. Such results suggest that milk production and content of lactating Shami goat can be changed by a level of 5% of Ca salts.

Analysis Of The Orf Region Of The Prion Protein Gene In The Botosani Karakul Sheep Breed From Romania, Steliana Elvira Maria Kevorkian, Mihaela Zaulet, Maria Adina Manea, Sergiu Emil Georgescu, Marieta Costache

Turkish Journal of Veterinary & Animal Sciences

Ovine scrapie is considered a neurodegenerative disease characterized by the accumulation of an abnormal, protease-resistant isoform of PrP. The polymorphisms at codons 136, 154, and 171 are associated with susceptibility to scrapie. In order to detect the existing polymorphisms at the 3 codons we used the real-time PCR technique. The obtained genotypes were confirmed by PCR amplification and sequencing of a 402 bp fragment of exon 3 of the prion gene. Known polymorphisms were detected at codons 136, 154, and 171 and 5 different genotypes were determined. The results showed that all animals were homozygous for A at codon 136 …

The Effect Of Ovine Placenta Extract, On Mammogenesis, Lactogenesis And Galactopoiesis In Sheep, Gabriel Cotor, Aneta Pop, Marian Ghita

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to evaluate the effect of ovine placenta extract, obtained by affinity chromatography on immobilized wheat germ lectin, on mammogenesis, lactogenesis, and galactopoiesis in pregnant and lactating sheep. Placental extract (100 \mug/mL protein concentration) was subcutaneously injected to pregnant sheep in days 1, 4, 7, 10, and 13 of the experiment (totally 10 mL placenta extract/animal) alternatively on the right and left side of the neck. Placental extract induced a more intense development of udder parameters; udder circumference increase was of 16.73%, anteroposterior length increase was of 15.9%, and latero-lateral length of 11.1%. Placental …

Acetic Acid Modulates Induction Of Pulmonary Hypertension In Broiler Chickens: Based On Electrocardiographic Parameters, Hossein Hassanpour, Abdolkarim Zamani Moghadam, Hamed Zarei, Neda Taghavi

Turkish Journal of Veterinary & Animal Sciences

To clarify the effect of acetic acid on the electrocardiographic parameters of pulmonary hypertensive broilers, chicks were reared at high altitude and treated with acetic acid (0.4 and 0.9 g/L) for 45 days. The right ventricle-to-total ventricle ratio was noted as an index of pulmonary hypertension that was significantly (P < 0.05) decreased in the treated groups. S amplitudes were significantly decreased only at 36 days (leads II and III) and 45 days (lead aVF) in many treated groups. There were significant (P < 0.05) reductions of T amplitudes at 28 days (leads aVR and aVL) and 36 days (lead aVL), and significant elevations of QRS (leads II, III, and aVF) and QT intervals (leads II, aVR, aVL, and aVF) at different ages of many treated groups. R amplitudes were significant (P < 0.05) at 28 days (leads aVL and aVF, at both doses of acetic acid), 36 days (leads aVR and aVL, at 0.4 g/L of acetic acid), and 45 days (lead aVR, at both doses of acetic acid). The RR interval also significantly increased at 28 days (leads aVR and aVL) and 45 days (lead aVL). It was concluded that acetic acid modulates induction of ventricular hypertrophy, dilation, and arrhythmia. These effects are detectable in many electrocardiographic parameters.

Haematological And Biochemical Values Of The Blood Of Pheasants (Phasianus Colchicus) Of Different Ages, Tufan Keçeci̇, Ramazan Çöl

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine selected haematological and biochemical parameters of pheasants (Phasianus colchicus) bred in Konya city in Turkey. Forty-five healthy pheasants of different ages (1, 5, and 12 months old) were examined. In the blood samples taken from these animals, haematological and biochemical values were determined. Comparing the haematological and biochemical parameters among the age groups, red blood cell counts, haemoglobin amount, haematocrit values, lymphocyte percentage, and total protein and albumin levels increased with the advancement of age. However, glucose, length and width of erythrocyte, length and width of erythrocyte nucleus, and percentages of monocyte and basophile …

Effects Of Dietary Fish Oil Replacement By Cottonseed Oil On Growth Performance And Fatty Acid Composition Of Rainbow Trout (Oncorhynchus Mykiss), Müjde Güler, Mustafa Yildiz

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine the effects of replacing fish oil (FO) with cottonseed oil (CSO), as an alternative plant lipid source in diets for rainbow trout (89.3 ± 1.1 g mean individual weight), on the growth and fatty acid composition of fish. As a control diet, 5 experimental diets were formulated with pure FO and with partial or complete (25%, 50%, 75%, or 100% CSO, respectively) replacement of FO with CSO. The fish were fed 2% of their body weight per day for 60 days at an average water temperature of 10.2 °C. At the …

Monitoring Of Somatic Cell Count Variations During Lactation In Primiparous And Multiparous Turkish Saanen Goats (Capra Hircus), Abdülkadi̇r Orman, Ayteki̇n Günay, Faruk Balci, Mehmet Koyuncu

Turkish Journal of Veterinary & Animal Sciences

In this study, a total of 28 Saanen does were used to evaluate the relationship between somatic cell count (SCC) and milk yield. The study included 14 first-lactation (primiparous) does and 14 does in second lactation or greater (multiparous). Individual milk samples from both morning and evening milkings were taken every 15 days throughout the lactation period of 7 months. While somatic cell counts were higher during the evening milking (840.40 versus 532.30 cells mL-1; 1050.40 versus 759.70 cells mL-1), milk yields were lower during the evening milking (115.40 versus 193.80 kg; 177.40 versus 280.00 kg) for both primiparous and …

The Increase Of Cd4+Cd25+ T Cells In The Peripheral Blood Of Pigs Persistently Infected With Porcine Reproductive And Respiratory Syndrome Virus, Jingwen Zeng, Danting He, Xin Guo, Xinna Ge, Hanchun Yang

Turkish Journal of Veterinary & Animal Sciences

Regulatory T lymphocytes (T-regs) may be involved in suppressing the host's immunity, which is important for viral clearance, and may therefore contribute to persistent viral infection. However, nothing is known regarding the situation of porcine T-regs during persistent infection with porcine reproductive and respiratory syndrome virus (PRRSV). This study, which used flow cytometry and real-time PCR, determined that the frequencies of CD4+CD25+ T-cells and porcine Forkhead/winged helix transcription factor (FoxP3) mRNA levels in peripheral blood significantly increased during PRRSV persistence, indicating that CD4+CD25+ T-regs might play an important role in PRRSV persistent infection. This study contributes to a better understanding …

Population Ecology Of A Phthirapteran Occurring On The Common Baya (Ploceus Philippinus) (Ploceidae: Passeriformes: Aves), Gaurav Arya, Nayanci Bansal, Aftab Ahmad, Nidhi Gupta, Arun Kumar Saxena

Turkish Journal of Veterinary & Animal Sciences

One hundred common Bayas (Ploceus philippinus) were examined for their ectoparasites, during April 2006 to December 2008 in the Rampur district of India (28°49'12"N 79°1'11"E). Seventy-four animals were found infested (mean intensity 13.97, sample mean abundance 10.3, range of infestation 1-45) with an ischnoceran louse, Brueelia plocea. The frequency distribution pattern of the louse on the aforesaid bird was aggregated but did not conform to the negative binomial model. The sex ratio was female biased while the nymphal population was dominant over adults.

Comparison Of Placentome Diameters In Single And Twin-Pregnant Sheep By Ultrasonographic Method, Güven Kaşikçi, Özge Yilmaz, Mehmet Can Gündüz, İsmai̇l Kirşan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the correlation between fetal number and placentome diameters in pregnant sheep. In the present study 25 Kıvırcık mixed breed pregnant ewes were used. Pregnancy of the ewes was diagnosed by transrectal ultrasonography by the 25th day after insemination. A transabdominal probe (6.5 MHz) was used on the pregnant ewes at the 30th day. At this time the placentomes appeared as hypoechogenic areas with a "C"-like shape. Throughout the pregnancies 5 placentomes were measured from each ewe every week. The results showed no significant difference between placentome diameter size in single and twin-pregnant …

Effects Of Halving Intravaginal Sponges And Ecg Dose On Estrus Response And Fertility In Tahirova Ewes During The Breeding Season, Güven Kaşikçi, Ümüt Ci̇ri̇t, Mehmet Can Gündüz, Süleyman Bacinoğlu, Ahmet Sabuncu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the effect of halving the intravaginal sponges impregnated with 20 mg of flugestone acetate (FGA; 20 or 10 mg) and a dose of equine chorionic gonadotropin (eCG; 600 or 300 IU) on fertility in Tahirova ewes. A total of 200 Tahirova ewes were randomly assigned to 1 of 2 groups during the breeding season. Half of the ewes (n = 100) were treated with whole intravaginal sponges (WS, 20 mg of FGA) and the other half (n = 100) with halved sponges (HS, 20 mg of FGA) for 14 days. The ewes …

Comparative Ultrasonographic Study Of The Prostate Complex And Bulbourethral Glands Of The Domestic Rabbit (Oryctolagus Cuniculus), Rosen Dimitrov, Kamelia Stamatova

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to provide a comparative description of the rabbit prostate complex and bulbourethral glands by means of ultrasonography, in order to obtain results that may be useful for the diagnosis of reproductive pathology in this species. The prostate and bulbourethral glands were studied sonographically in 12 sexually mature, clinically healthy male New Zealand rabbits that were 12 months old and weighed 2.8 to 3.2 kg. The prostate complex, composed of proprostate, prostate, and paraprostate parts, was observed using prepubic ultrasonography in sagittal and transversal planes and presented a hyperechoic stroma. The bulbourethral glands were …

Massive Toxoplasma Abortions In A Dairy Sheep Flock And Therapeutic Approach With Different Doses Of Sulfadimidine, Nektarios D. Giadinis, Konstantinos Terpsidis, Anastasia Diakou, Victoria Siarkou, Panayiotis Loukopoulos, Ridvan Osman, Harilaos Karatzias`, Margarita Papazahariadou

Turkish Journal of Veterinary & Animal Sciences

In a 500 head dairy sheep flock in Northern Greece, massive abortions (60%) were observed at 110-130 days of gestation. Attempts were made to treat the abortions with oxytetracyclin L.A. 20% but they were not successful. Toxoplasmosis was confirmed by laboratory examination of blood sera of ewes, aborted fetuses, and fetal brain samples. The remaining 200 pregnant sheep were divided in 2 groups of 100 ewes each. Animals of Group 1 were treated with 20 mg/kg b.w. sulfadimidine every other day 4 times, while the animals in Group 2 received 33 mg/kg b.w. sulfadimidine every other day 4 times via …

Mechanical Evaluation Of Aluminum Oxide Roughened Screws In Equine Third Metacarpal Bone, Petrisor Baia

LSU Master's Theses

ABSTRACT Objectives – To compare the osseointegration of roughened and electropolished 5.5 mm cortical screws used to secure a 4.5 mm broad dynamic compression plate (DCP) in equine third metacarpal (MC3) bones. Study Design – In vivo study Animals – 5 Adult thoroughbred horses (2-7 years old). Methods – For each horse one MC3 was randomly assigned to secure a 4.5 mm broad DCP with 4aluminum oxide (Al2O3) roughened screws on the dorsal mid diaphysis. Four regular electropolished screws used to secure a similar plate on the contralateral limb served as control. They were removed at 12 weeks and the …

The Role Of Inflammatory Molecules In Hypertension, Jeffrey Paul Cardinale

LSU Doctoral Dissertations

Hypertension is a serious condition with high morbidity and mortality rates, as well as a major predisposing factor for multiple cardiovascular and renal diseases. Angiotensin II (Ang II) of the renin-angiotensin system (RAS) plays a pivotal role in propagating the hypertensive response. Moreover, hypertension is considered a chronic low-grade inflammatory condition that intimately involves the actions of proinflammatory cytokines (PICs) such as tumor necrosis factor-alpha (TNF). Recent evidence highlights the role that inflammation, specifically TNF, plays in hypertension and in regulating the RAS, but the understanding as to how inflammation is regulated following Ang II or TNF activation, as well …

Characterization Of Spiroplasma Mirum And Its Role In Transmissible Spongiform Encephalopathies, Hilari Maree French

LSU Doctoral Dissertations

There are many contradictory reports in the literature involving the culture of Spiroplasma mirum, its resistance to disinfectants and antibiotics, and its potential role in Transmissible Spongiform Encephalopathies (TSEs). These contradictions led to an interest in a possible link between spiroplasma and TSEs and the development of a research plan to elucidate this connection. It was hypothesized that Spiroplasma was associated with a neurodegenerative disease such as TSE. In this work we further characterized S. mirum laboratory strains and continued to evaluate the possible correlations they have with TSE infections and the prion. To optimize recovery of S. mirum from …

Arginine Metabolism In The Edwardsiella Ictaluri- Channel Catfish Macrophage Dynamic, Wes Arend Baumgartner

LSU Doctoral Dissertations

Edwardsiella ictaluri encodes a urease operon and an arginine decarboxylase (AdiA) that are required for virulence in head kidney derived macrophages (HKDM). The urease produces ammonia in amounts sufficient to alter environmental pH from acid to neutral. A hypothetical model was proposed, involving arginine metabolism in E. ictaluri infected HKDM, focusing on bacterial urease, AdiA, a second arginine decarboxylase (SpeA), and agmatinase (SpeB). Using fluorescence based ratiometric pH determination of E. ictaluri in live HKDM, it was shown that E. ictaluri modulates HKDM phagosome pH to above six. Urease and AdiA mutants failed to up-regulate vacuole pH, while vacuole pH …

Cystic Ovaries In Dairy Cattle, Lacey Morgan Rosenberg

Dairy Science

The objective of this literature review was to determine the potential causes and factors affecting the incidence of ovarian cysts in dairy cattle as well as to examine functionality of various preventative methods and treatments of cysts. The end product was to be a document outlining all the information available on the three different types of cysts that can develop on the bovine ovary, follicular cysts, luteal cysts, and cystic corpora lutea. The literature that was reviewed, most in the forms of research papers, articles, and reviews, came primarily from scientific journals and published books. The literature reviewed was only …

Characterization Of The Sero-Reactivity Of Proteins Map1152 And Map1156 From Mycobacterium Avium Subspecies Paratuberculosis, Avery L. Paulson

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease (JD) in ruminants. Development of genetic tools and completion of the MAP genome sequencing project expanded opportunities for antigen discovery. In this thesis, I review the current trends in diagnosis and disease control of JD and present the results of the studies on the seroreactivity of two proteins encoded for by the MAP1152-MAP1156 gene cluster. MAP1152 encodes for a PPE protein and MAP1156 encodes a diacylglycerol acyltransferase involved in triglyceride metabolism and classified in the uncharacterized protein family UPF0089. Maltose-binding protein (MBP) tagged recombinant MAP proteins were purified from Escherichia coli. …

Staphylococcus Aureus Virulence Factors Synthesis Is Controlled By Central Metabolism, Yefei Zhu

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Staphylococcus aureus is a versatile pathogen that can survive in diverse host environments. This versatility depends on its ability to sense nutrients and respond by modulating gene expression, including the synthesis of virulence determinants. In addition to its ability to synthesize virulence factors, the capacity of S. aureus to form biofilms is an important mediator of virulence in certain infections. Biofilms are a complex aggregation of bacteria commonly encapsulated by an adhesive exopolysaccharide matrix (polysaccharide intercellular adhesin; PIA). To study S. aureus biofilm formation, we assessed the metabolic requirements of S. aureus growing in a biofilm and …

How I Became A Scientist: Activity Book For 3rd Graders, Jessica Schneider, Kauline Davis, Thad Blossom

College of Veterinary Medicine eBooks

This activity book highlights how eight people became scientists from all over the world who work with animals. These scientists work hard to make sure that people and their animals stay healthy. Activities in the book cover topics related to each scientist's area of study.

Published in full color, the book features fun activities relevant to the different scientific specialties represented for children to complete. The publication of the book has been supported by the National Institutes of Health, the National Center for Research Resources, and a Scientific Education Partnership Award.

Disseminated Tuberculosis In Captive Malayan Tapir (Tapirus Indicus), Theerayuth Kaewamatawong, Wijit Banlunara, Anudep Rungsipipat, Nopadon Pirarat, Suphasawatt Puranaveja, Angkana Sommanustweechai

The Thai Journal of Veterinary Medicine

Tuberculosis caused by Mycobacterium tuberculosis is an important zoonotic disease that concerns the survival of endangered species and public health aspects. A 17-year-old, female Malayan tapir from a zoo in Thailand died with a sign of panting after anesthesia for the treatment of her oral problems. Grossly, multiple caseous white nodules were scattered throughout the parenchyma and bulged from the capsular surface in affected organs such as lung, lymph node and liver. Histologic examination revealed multifocal to coalescing granulomas characterized by caseous necrotic center, loosely organized inflammatory zone with low numbers of Langhans’s giant cells and thin fibrotic zone. A …

Tetranucleotide Microsatellite Markers For Molecular Testing In Thai Domestic Elephants (Elephas Maximus Indicus), Duangsmorn Suwattana, Jutarat Jirasupphachok, Sumolya Kanchanapangka, Weerapong Koykul

The Thai Journal of Veterinary Medicine

Eleven microsatellite loci were evaluated for genetic profiles of Thai domestic elephants (Elephas maximus indicus) and their suitability as genetic markers for molecular testing. A total of 66 animals were tested. 10 out of 11 loci could be amplified and they demonstrated polymorphism with allelic numbers per locus ranging from 7 (LaT06) to 39 (LaT18). Values of expected heterozygosity (He) and Polymorphic Information Content (PIC) were between 0.6449 (LaT17) – 0.9593 (LaT05) and 0.5934 (LaT17) – 0.9578 (LaT05), respectively. Analysis of the ten microsatellite markers revealed Combined Exclusion Probability (CEP) of 99.99998783% or 1.2167 x 10-7 and 99.91% confident for …

An Outbreak Of Avibacterium Paragallinarum Serovar B In A Thai Layer Farm, Kridda Chukiatsiri, Suwit Chotinun, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Avibacterium paragallinarum serovar B was diagnosed and revealed in a layer farm. The infected chickens showed mucous nasal discharge, facial edema and conjunctivitis. Egg production also decreased dramatically. Necropsy findings showed lesions of conjunctivitis and edematous tissue around the eyes. The infraorbital sinuses were filled with a caseopurulent mass and tracheitis. Bacterial isolation and identification was performed and confirmed by PCR technic. A hemagglutination inhibition test using specific antibodies against serovar B revealed the Avibacterium paragallinarum serovar B infection. A pathogenicity test done in 10 layer chickens, showed clinical signs of nasal discharge, facial edema and conjunctivitis. This is the …