Veterinary Medicine Commons^™

Cereal Rye, Winter Triticale Or Winter Wheat: Which Is Best For Early Spring Grazing?, Alyssa K. Hall, Abigail M. Sartin, Kallie J. Calus, Morgan T. Grabau, Daren D. Redfearn, Robert B. Mitchell, Mary E. Drewnoski

Nebraska Beef Cattle Reports

A study was conducted to determine which winter-hardy small cereal grain was best suited for early spring grazing. Three species were evaluated: winter wheat, cereal rye, and winter triticale, as a double-cropped forage in a continuous soybean rotation. Within this rotation the number of grazing days is limited, but all three species provided high rates of cattle weight gain, with an average daily gain (ADG) of 3 lb/d in April. However, in a year where freezing conditions occurring after cattle started grazing, cattle grazing cereal rye had the greatest ADG, likely due to greater forage growth. Thus, cereal rye may …

Vitamin A In Cow-Calf Production: Impacts Of Maternal Supplementation And Status On Offspring, Hannah F. Speer, Harvey C. Freetly, Karla H. Jenkins, Mary E. Drewnoski

Nebraska Beef Cattle Reports

The young calf is at greatest risk of vitamin A deficiency when cow vitamin intake is low in late pregnancy. Two studies were conducted to evaluate the relationship between cow and calf vitamin A status and how vitamin A status of cow-calf pairs was influence by maternal vitamin A supplementation. In general, calves did not have adequate liver vitamin A concentrations despite cows having adequate liver vitamin A stores following calving. Both cow iver stores and cow vitamin A intake during late gestation influence the amount of vitamin A in colostrum, so it benefits the calf if the cow has …

The Effect Of Postpartum Supplementation Strategy On Performance In May-Calving 2- And 3-Yr-Old Range Cows, Nicole M. Woita, Selby L. Boerman, J. A. Musgrave, Kacie L. Mccathy, J. Travis Mulliniks

Nebraska Beef Cattle Reports

Reproductive performance in young 2- and 3-yr-old cows are often the lowest in the cow herd, which is due to their inability to consume enough energy and protein to meet their requirements for growth and lactation. A 3-yr study was conducted to evaluate the effects of postpartum supplementation strategy on reproduction, cow body weight, and calf performance in lactating young 2- and 3-yr-old May-calving range cows. Supplementation was initiated 20 d prior to the start of the breeding season (45-d postpartum) and continued throughout the 45-d breeding season (125-d postpartum). Supplementation was provided daily with treatments being 1) mineral supplement …

The Impact Of Prepartum Supplementation Strategy On Cow-Calf Performance In May-Calving Mature Cows, J. A. Musgrave, Kacie L. Mccathy, J. Travis Mulliniks

Nebraska Beef Cattle Reports

Implementation of minimal supplemental protein strategies during late gestation may have the potential to minimize any negative postweaning progeny performance while decreasing feed costs. A 3-yr study was conducted to evaluate the effects of mid- to late-gestation supplementation strategies on reproduction, cow body weight, and calf performance in May-calving mature range cows grazing upland native range. Supplementation was provided 2x weekly with treatments being 1) 05 lb per day of a 30% CP distillers-based supplement, 2) 1 lb per day of a 30% CP distillers-based supplement, 3) a negative control as a flexible supplementation strategy. The Flex strategy was developed …

The Effect Of Late Gestation Supplementation Strategy On Cow-Calf Performance In March-Calving Mature Cows, J. A. Musgrave, Kacie L. Mccathy, J. Travis Mulliniks

Nebraska Beef Cattle Reports

Implementation of supplemental protein strategies during late gestation has been shown to positively affect postweaning progeny performance. A 2-yr study was conducted to evaluate the effects of late gestation supplementation strategies on reproduction, cow body weight, and calf performance in March-calving mature range cows grazing dormant upland range. Supplementation was individually fed and provided daily with treatments being: 1) no supplementation, 2) 2 lb per day of a 30% CP distillers-based supplement, 3) 2 lb per day of a 30% CP distillers-based supplement with the inclusion of 160 mg/cow daily of monensin, 4) 2 lb per day of a 30% …

Effect Of Glucogenic Feed Additive On Reproductive Performance In Young Postpartum Range Cows, Tasha M. King, J. A. Musgrave, Nicole M. Woita, Shelby L. Boerman, J. C. Macdonald, J. Travis Mulliniks

Nebraska Beef Cattle Reports

Performance of young March-Calving range cows receiving a protein supplement with the addition of either monensin or propionate salt were compared to evaluate the effect of feed additive on overall production in the postpartum stage. cow body weight and body condition were not impacted by postpartum supplementation throughout the study. Calf body weights were not impacted by type of feed additive at birth, weaning, or 205-d. Twenty-four-hour milk production was not impacted by the type of feed additive. Conception rates for cows receiving postpartum supplementation containing propionate salt were greater than cows receiving monsensin. This implies that the addition of …

Effect Of Heifer Percent Mature Body Weight At Breeding On Heifer Performance, Calf Production, And Subsequent Pregnancy Rates, Josie N. Crouch, J. Travis Mulliniks, J. A. Musgrave, Kathy J. Hanford, Kacie L. Mccathy

Nebraska Beef Cattle Reports

A retrospective study was done utilizing 1,434 March- and May- calving Red Angus x Simmental crossbred cows and heifers from 2005 through 2019. Heifer weight as a percentage of mature body weight at breeding was used to conduct a regression analysis from 50 to 70% of mature body weight to determine the impact of body weight at breeding on reproductive performance. Heifer pregnancy rates and second pregnancy rates as 2-year-olds were greater for heifers at 60, 65, and 70% of mature body weight at breeding than heifers at 50 and 55%. However, heifer percent mature body weight at breeding did …

Impact Of Increasing Level Of Milk Production On Cow-Calf Performance In Nebraska Sandhills, Selby L. Boerman, Jacki A. Musgrave, Kathryn J. Hanford, Mitchell B. Stephenson, J. Travis Mulliniks

Nebraska Beef Cattle Reports

In a 2-yr study, data were collected on 118 crossbred cow-calf pairs from March and May-calving herds. On approximately 30, 60, 90, 120, and 210 d postpartum, individual cow 24-h bilk yield was estimated through weigh-suckle weigh techniques. Cow body weight (BW) and body condition score (BCS) were collected weekly through breeding. Calf BW was recorded at each milking. Individual cow milk area under the curve (AUC) values were calculated and data were analyzed using linear regression analysis. Results from this study illustrate that increasing total milk produced throughout the lactation period had minimal influence on the cow production parameters …

Distribution Pattern And Risk Factors Of Foot And Mouth Disease Outbreaks In Java Island, Indonesia, Virgilius Martin Kelake Kedang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Indonesia experienced new outbreaks of foot and mouth disease (FMD) in 2022 after being officially free from the disease for several decades. The outbreak started in April 2022 in East Java and rapidly spread to many regions in Indonesia. This study aimed to investigate the distribution pattern and risk factors associated with the FMD outbreaks in Java Island, Indonesia, from 2022 to 2023. Descriptive, spatial, spatiotemporal, and risk factor analyses were employed to examine the outbreak dynamics and contributing factors. The results indicated that the outbreaks were distributed across the island, with East Java being the most affected region. The …

Epidemiological And Molecular Studies On Lumpy Skin Disease Outbreaks In Beef Cattle In Thailand During 2021-2022, Phurida Sripipattanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lumpy skin disease (LSD) is a vector-borne viral disease of cattle caused by the Lumpy skin disease virus (LSDV). LSD is considered as an emerging infectious disease in cattle in Asia and poses a significant threat to cattle production across Africa, Europe, and Asia. This study aimed to describe the epidemiological characteristics of LSD outbreaks in beef cattle in Thailand and to conduct a molecular survey of LSDV infection in beef cattle from May 2021 to December 2022. The study included 594 beef cattle from 257 farms affected by the LSD outbreaks in 28 provinces. We collected hair samples (n=492) …

Comparative Study Of The Effects Of Dietary Supplements On Reproductive And Growth Performance In Sows And Nursery Pigs, Panumas Kongpanna

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study evaluated the effects of dietary supplementation with a multi-strain probiotic containing Bacillus subtilis (5 × 107 CFU/g) and Lactobacillus plantarum (1 × 109 CFU/g) on reproductive performance in multiparous sows and growth performance in weaned piglets. In Experiment 1, 300 sows were assigned to control or probiotic diets across five parity groups (2, 3, 4, 5 and 6 to 9) using a 2 × 5 factorial design. Experiment 2 involved 120 weaned piglets randomly allocated to four dietary treatments: control, antibiotic, enzyme, or probiotic. Measurements included reproductive and litter performance, colostrum IgG, milk composition, and piglet growth, digestibility, …

A Novel Non-Rodent Animal Model Of Hydrochloric Acid-Induced Acute And Chronic Lung Injury, Pavel A. Solopov, Ruben Manuel Luciano Biancatelli, Tierney Day, Christiana Dimitropoulou, John D. Catravas

Bioelectrics Publications

Hydrochloric acid is one of the most prevalent and hazardous chemicals. Accidental spills occur in industrial plants or during transportation. Exposure to HCl can induce severe health impairment, including acute and chronic pulmonary diseases. We have previously described the molecular, structural, and functional aspects of the development of chronic lung injury and pulmonary fibrosis caused by intratracheal instillation of HCl in mice. Although mouse models of human disease have many advantages, rodents are evolutionary far from human and exhibit significant anatomical and physiological differences. Genetic and anatomic similarities between rabbits and humans are significantly higher. Rabbit models of HCl-induced lung …

Toxicity Of Crude Oil-Derived Polar Unresolved Complex Mixtures To Pacific Herring Embryos: Insights Beyond Polycyclic Aromatic Hydrocarbons, Maxwell L. Harsha, Yanila Salas-Ortiz, Alysha D. Cypher, Ed Osborn, Eduardo Turcios Valle, Jacob L. Gregg, Paul K. Hershberger, Yuri Kurerov, Sarah King, Aleksandar I. Goranov, Patrick G. Hatcher, Anastasia Konefal, T. Erin Cox, Justin B. Greer, James P. Meador, Matthew A. Tarr, Patrick L. Tomco, David C. Podgorski

Chemistry & Biochemistry Faculty Publications

Crude oil toxicity to early life stage fish is commonly attributed to polycyclic aromatic hydrocarbons (PAHs). However, it remains unclear how the polar unresolved complex mixture (UCM), which constitutes the bulk of the water-soluble fraction of crude oil, contributes to crude oil toxicity. Additionally, the role of photomodification-induced toxicity in relation to the polar UCM is not well understood. This study addresses these knowledge gaps by assessing the toxicity of two laboratory generated polar UCMs from Cook Inlet crude oil, representing the readily water-soluble fraction of crude oil and photoproduced hydrocarbon oxidation products (HOPs), to Pacific herring (Clupea pallasii …

Evidence Synthesis Methodology: Promoting Reproducible Methodology Through Modified Assignments, Laura Lipke, Neyda Gilman

Library Created Resources

Initially, systematic reviews in healthcare served to synthesize findings from clinical studies, aiding comprehensive access to the most effective treatments within evidence-based practice. Over time, their scope has broadened beyond treatment effectiveness, leading to a surge in publication. Consequently, many health science faculty now assign systematic reviews in classrooms without a full grasp of the standardized process, leaving students ill-equipped due to insufficient guidance and resources.

While mastering systematic review techniques is crucial for evidence-based practice, faculty can opt for modified assignments to foster a deeper understanding of the review process. These modified tasks, can be completed within a semester, …

Current And New Perspectives On Equine Protozoal Myeloencephalitis Therapeutics, Izabela De Assis Rocha

Theses and Dissertations--Veterinary Science

Equine protozoal myeloencephalitis (EPM) is an important neurologic disease of horses in the Americas. It is caused by the apicomplexan parasite Sarcocystis neurona and less commonly by the related parasite Neospora hughesi. Current antiprotozoal treatment relies on the use of either folate inhibitors (sulfadiazine/pyrimethamine) or triazine drugs (diclazuril/ponazuril), with a success rate of 60-70% regardless of the drug of choice. Successful treatment is defined as the improvement of at least one ataxia grade following the modified Mayhew scale, which often is not enough for the horse to return to the previous level of performance. The goals of this dissertation …

Statistics Used In The Nebraska Beef Reports And Their Purpose, University Of Nebraska-Lincoln

Nebraska Beef Cattle Reports

The purpose of beef cattle and beef product research at UNL is to provide reference information that represents the various populations (cows, calves, heifers, feeders, carcasses, retail products, etc) of beef production. Obviously, the researcher cannot apply treatments to every member of a population; therefore he/she must sample the population. The use of statistics allows the researcher and readers of the Nebraska Beef report the opportunity to evaluate separation of random (chance) occurrences and real biological effects of a treatment. Following is a brief description of the major statistics used in the beef report. For a more detailed description of …

Evaluation Of Vivalto® On Growth Performance And Carcass Characteristics In Growing And Finishing Beef Steers, Garrett N. Lemon, Rebecca Mcdermott, Galen E. Erickson, Jeff Heldt, Pablo L. Loza

Nebraska Beef Cattle Reports

A finishing trial conducted at the Pan-handle Research, Extension, and Education Center (PREEC) near Scottsbluff, NE evaluated the effect of Vivalto^®, a B-vitamin complex, on the feeding performance and carcass characteristics of feedlot steers. The design of the study was completely randomized block design with three treatments, control without Vivalto^®, 1g Vivalto^®/steer/day, and 2g Vivalto^®/steer/day. Cattle fed the diets that included Vivalto^® feed had lower intakes during the first 56 days than cattle that received no Vivalto^® in the diet. No significant differences were observed for ADG, intake, feed efficiency …

Effect Of Biochar On Enteric Methane Production And Cattle Performance, Hanna Cronk, Holly Heil, Rebecca Mcdermott, Mitch Norman, Galen E. Erickson, Andrea Watson

Nebraska Beef Cattle Reports

Methane is a more potent greenhouse gas than carbon dioxide. Because ruminant animals, including cattle, emit methane, strategies are actively being sought to reduce these emissions. Pistachio shell-sourced biochar was included at 0.5% of a finishing cattle diet to determine effects on methane emissions and cattle performance. Eight pens of cattle were rotated through a 2-chambered emissions barn to analyze methane and carbon dioxide produced by the cattle. Biochar tended to increase methane emissions on a g/d basis with no effect on methane emissions as g/lb of feed intake and no differences in carbon dioxide emissions. There were no differences …

Impact Of Strip-Grazing Stockpiled Annual/Cover Crop Forages On Carrying Capacity And Animal Performance, Shelby L. Davies-Jenkins, Abigail Sartin, Devin Jakub, Zac Carlson, Erin Laborie, Jack Arterburn, Ben Beckman, Brad Schnick, M. E. Drewnoski

Nebraska Beef Cattle Reports

Annual forages/cover crops can be used to fill the fall/winter grazing gap, and strip grazing may increase carrying capacity by reducing trampling losses of the forage. The current experiment utilized a series of on-farm experiments across two growing seasons to compare continuous and strip grazing of various summer planted cover crops. Strip grazing increased carrying capacity by an average of 47 ± 15% and gain per acre by 44 ± 5% compared to continuous grazing although significant variability in the amount of increase was observed. This variability can likely be attributed to forage type (quality), frequency of moves, and forage …

Strategies For Ddgs Supplementation Frequency To Frazing Yearling Streers, Sydney T. Vanderhoff, Rebecca L. Sjostrand, Mitchell M. Norman, Isaque F. Vicci, Jim C. Macdonald, Mary E. Drewnoski

Nebraska Beef Cattle Reports

Infrequent supplementation saves labor and may reduce animal performance, but recent research with reduced fat distillers grains has provided inconsistent results. This experiment evaluated the effects of daily and three times a week supplementation of dried distillers grains on yearling steer performance grazing smooth bromegrass pastures from May to August. Daily supplemented steers received 5.6 pounds of dry matter per steer of dried distillers grains with solubles 7 days/week. The three times a week supplemented steers received 13.0 pounds of dry matter per steer of dried distillers grains with solubles three days/week (Monday, Wednesday, Friday). A control treatment received no …

Effect Of Methionine Supplementation During Late Gestation In Beef Females, Landon F. Tadich, Jim R. Teichert, Kathryn J. Hanford, Jacki A. Musgrave, Rich N. Funston

Nebraska Beef Cattle Reports

Some amino acids are known to be essential to cattle and effect protein availability to the animal, especially during gestation when nutrient requirements are higher. Methionine is found to be one of the most limiting in low quality forage diets. Two 3-yr studies were performed to evaluate the impact of methionine supplementation during late gestation on intake, body weight, average daily gain, and subsequent calf performance in primiparous and multiparous females. In exp 1, 120 artificially inseminated pregnancy heifers were placed in a Calan gate feeding system (n=40/yr) and assigned 1 of 3 treatments during late gestation and fed ad …

Artificial Insemination Of Beef Heifers With Multi-Sire Semen, Dempster Christenson, Jordan Thomas, Daniel J. Kelly, John G. Maddux, R. Funston

Nebraska Beef Cattle Reports

The study compared pregnancy rates of beef heifers artificially inseminated with multi-sire semen to single-sire semen at ranch 1 and demonstrated pregnancy to multi-sire sexed semen at rand 2. It was hypothesized pregnancy rates resulting from multi-sire semen would be increased compared to single-sire semen. Ranch 1 heifers were inseminated with either single-sire or multi-sire semen and all heifers expressing estrus at ranch 2 were inseminated with multi-sire semen averaged numerically greater pregnancy rate than the average single-sire pregnancy rate and pregnancy outcomes from multi-sire sexed semen exceed previous literature but cannot be directly compared. Despite similar pregnancy results between …

Long-Term Performance Of Steers Grazing Smooth Bromegrass Pastures, Morgan Hodges, Holly Heil, Rebecca Mcdermott, Anastasios Mazis, Tala Awada, Galen E. Erickson, Andrea K. Watson

Nebraska Beef Cattle Reports

Summary with Implications

Body weight gain of yearling steers grazing smooth bromegrass pastures was measured across 17 years from 2005 to 2021. Three treatments were applied, (1) control pastures with no additional inputs, (2) pastures fertilized with 80 lb of N per acre, and (3) pastures grazed with cattle supplemented daily with approximately 5 lb of dry distillers grains. The cnntrol treatment pastures (1.4 acres/steer) were stocked 30% lighter than the fertilized and supplemented pastures (1.0 acre/steer). Across the 17 years, the supplemented cattle gained 2.24 lb/day while the non-supplemented cattle (both control and fertilized treatments) gained 1.57 lb/day. Body …

Strategies Of Utilizing Sex-Sorted Semen To Improve Beef Cattle Herd Production And Using Lasia Spinosa Thw. And Zinc Supplementation To Improve Semen Quality Of Bulls And Boars With Poor Semen Profile, Supitcha Kaewma

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of the thesis were i) to improve the success of using sex-sorted semen and ii) to enhance the semen quality in low semen quality bulls and boars. Four experiments were conducted to test the hypothesis as 1) on-farm factors influence the success of sexed semen artificial insemination (AI) in cattle herds, 2) estrus synchronization protocols can be integrated in sex-sorted semen AI in cattle herd, 3) Lasia spinosa Thw. (LST) supplementation for improving semen quality in bull and boar sires with poor semen quality, 4) zinc supplementation for improving semen quality in subfertile boar. Study 1: Experiment 1, …

Effect Of Haemonchus Contortus Excretory/Secretory Protein On Differences In Host Neutrophil Migration, Hannah Grace Teddleton

Graduate Theses, Dissertations, and Problem Reports (ETD)

An excretory/secretory (E/S) product, common to many helminths, is neutrophil inhibitory factor (NIF) which negatively affects neutrophil migration and activity. Due to differences in neutrophil accumulation between parasite resistant St. Croix STC and parasite susceptible Suffolk (SUF) sheep, we hypothesized that Hc-NIF may inhibit migration of SUF and not STC neutrophils. Previous studies have demonstrated that Hc produces NIF but is not commercially available. To test our hypothesis, neutrophils were cultured from STC and SUF sheep in the presence of Ancylostoma caninum-derived NIF (Ac-NIF) and measure chemotaxis to Interleukin-8 (IL-8), HcLA or H. contortus third-stage excretory/secretory (E/S) products. Neutrophils were …

Steps Towards Operationalizing One Health Approaches, Kim M. Pepin, Keith M. Carlisle, Dean Anderson, Michael G. Baker, Richard B. Chipman, Jackie Benschop, Nigel P. French, Suzie Greenhalgh, Scott Mcdougall, Petra Muellner, Emil Murphy, Dion R. J. Neale, Michael J. Plank, David T. S. Hayman

United States Department of Agriculture Wildlife Services: Staff Publications

One Health recognizes the health of humans, agriculture, wildlife, and the environment are interrelated. The concept has been embraced by international health and environmental authorities such as WHO, WOAH, FAO, and UNEP, but One Health approaches have been more practiced by researchers than national or international authorities. To identify priorities for operationalizing One Health beyond research contexts, we conducted 41 semi-structured interviews with professionals across One Health sectors (public health, environment, agriculture, wildlife) and institutional contexts, who focus on national-scale and international applications. We identify important challenges, solutions, and priorities for delivering the One Health agenda through government action. Participants …

Changing Fire Regimes And Nuanced Impacts On A Critically Imperiled Species, Jeremy T. Rockweit, Katie M. Dugger, Damon B. Lesmeister, Raymond J. Davis, Alan B. Franklin, J. Mark Higley

United States Department of Agriculture Wildlife Services: Staff Publications

Wildfire activity throughout western North America is increasing which can have important consequences for species persistence. Native species have evolved disturbance-adapted traits that confer resilience to natural disturbance provided disturbances operate within their historical range of variability. This resilience can erode as disturbance regimes change and begin operating outside this range. We assessed wildfire impacts during 1987–2018 on the northern spotted owl, an imperiled species with complex relationships with late and early seral forest in the Pacific Northwest, USA. We analyzed population- and individual-level wildfire impacts across the frequent-fire portion of the owl’s geographic range at two spatial scales and …

Heating Decoys To Mimic Thermal Signatures Of Live Animals For Drones, Landon R. Jones, Cerise Mensah, Jared S. Elmore, Kristine O. Evans, Morgan B. Pfeiffer, Brad F. Blackwell, Raymond B. Iglay

United States Department of Agriculture Wildlife Services: Staff Publications

Method name

Heating animal decoys to mimic thermal signatures for drones

Abstract

Thermal sensors mounted on drones (unoccupied aircraft systems) are popular and effective tools for monitoring cryptic animal species, although few studies have quantified sampling error of ani- mal counts from thermal images. Using decoys is one effective strategy to quantify bias and count accuracy; however, plastic decoys do not mimic thermal signatures of representative species. Our objective was to produce heat signatures in animal decoys to realistically match thermal images of live animals obtained from a drone-based sensor. We tested commercially available methods to heat plastic decoys of …

Competitive Behaviors Between Black Vultures (Coragyps Atratus) And Turkey Vultures (Cathartes Aura) During Scavenging / Comportamientos Competitivos Entre Coragyps Atratus Y Cathartes Aura Durante El Carroñeo, Amanda M. Herbert, Patrick A. Zollner, Landon R. Jones, Marian L. Wahl, Grant N. Burcham, Bryan M. Kluever, Lee A. Humberg, Brandon M. Quinby

United States Department of Agriculture Wildlife Services: Staff Publications

Abstract

Interference competition occurs when an individual prohibits another’s access to a resource through antagonistic interactions. Despite considerable research on scavenger interactions at carrion, the limited evidence for competitive interactions between black vultures (Coragyps atratus) and turkey vultures (Cathartes aura) relies upon the frequency of antagonistic interactions without considering other forms of competition. Using remote cameras, we recorded the duration of aggression, scavenging, and vigilance behaviors exhibited by individual black vultures and turkey vultures scavenging stillborn domestic cattle carcasses within flocks composed of one or both vulture species. We tested for differences of durations of each …

The Texas Feral Swine Eradication And Control Pilot Program, David R. Pipkin, Bruce R. Leland, Katherine Garwood, Linda Tschirhart-Hejl, Michael J. Bodenchuk, John M. Tomecek

United States Department of Agriculture Wildlife Services: Staff Publications

The United States Agriculture Improvement Act of 2018 (the 2018 Farm Bill) established the Feral Swine Eradication and Control Pilot Program. The program funded US$75M for 5 years, split evenly between the Natural Resources Conservation Service (NRCS) and the Animal and Plant Health Inspection Service (APHIS), both programs within the United States Department of Agriculture. The agencies solicited joint programs from states with high densities of feral swine in two phases. In Texas, NRCS and APHIS submitted three multi-county project areas along watersheds for Phase I funding and one eradication effort along with two crop protection projects in Phase II. …