Veterinary Medicine Commons^™

Carbon Dioxide Laser-Assisted Staphylectomy In Brachycephalic And Nonbrachycephalic Dogs, Adolfo Maria Tambella, Cecilia Vullo, Fabrizio Dini, Angela Palumbo Piccionello, Anna Rita Attili

Turkish Journal of Veterinary & Animal Sciences

Elongated soft palate is a common upper airway anomaly in brachycephalic dogs. The scientific literature considering this pathology in nonbrachycephalic dogs is limited. The aim of this study was to estimate the importance of the elongated soft palate in nonbrachycephalic dogs (n = 10), carrying out, at the same time, a comparison with selected brachycephalic dogs (n = 19), on the effect of carbon dioxide laser staphylectomy. The selected population had only elongated soft palates and no other disorders. The surgery was rapid and uncomplicated; no suture material was used. The comprehensive recovery was significant (P < 0.0001): 68.4% of brachycephalic dogs and 100% of nonbrachycephalic dogs had complete recovery. The incomplete recovery in older brachycephalic dogs matched a more advanced pathologic phase. This study suggests that, in the case of high respiratory tract alteration and poor exercise tolerance, it is imperative to consider in differential diagnosis the elongated soft palate in nonbrachycephalic breeds, too; their clinical signs are less severe and frequently underestimated. The surgical treatment has a favorable prognosis when the only alteration is elongated soft palate. The carbon dioxide laser has been confirmed as an useful option for staphylectomy in dogs.

First Report Of Cryptosporidium Spp. And Giardia Duodenalis In Calves From Northeastern Brazil, Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, Ana Célia Rodrigues Athayde

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in cattle calves from the Sertão region of Paraíba State in northeastern Brazil. The experiment was conducted using 100 calves from 10 farms. To evaluate the presence of oocysts, the technique of flotation in a sucrose solution was used, and for detection of C. parvum and G. duodenalis, the immunochromatographic test RIDA®QUICK Cryptosporidium/Giardia strips were used. It was noted that animals of 1 to 30 days of age were statistically more affected (P < 0.05) by Cryptosporidium spp. Only 4 calves (25% of the positive animals) had diarrhea (P < 0.05). Nine animals were positive for G. duodenalis; diarrhea was present in 6 (66.67%) of those animals, which was considered a risk factor (P < 0.05). It was concluded that Cryptosporidium spp. and G. duodenalis were present in calves from northeastern Brazil.

Semen Quality In White Leghorn Chicken Selected For Egg Production Traits, Shanmugam Murugesan, Niranjan Matam, Rakesh Kulkarni, Tarun Kumar Bhattacharya, Rudranath Chatterjee

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to evaluate the effect of selection for egg production traits in White Leghorn chicken based upon the semen quality. Males of 3 pure lines of White Leghorn, namely IWH, IWI, and IWK, along with a pedigree random-bred control (IWC) population, were selected and housed in individual cages. The semen collected from individual birds was evaluated for different physical and biochemical parameters. There was significant (P =< 0.013) difference in semen quality parameters studied between the lines. High egg-producing lines IWH and IWI had poor semen quality in comparison with that of the control line (IWC). Thus, it can be concluded that selection for higher egg production affects the semen quality of males in the selected lines.

First Report Of Abnormal Pigmentation In A Surmullet, Mullus Surmuletus L. (Osteichthyes: Mullidae), Adnan Tokaç, Okan Akyol, Celaletti̇n Aydin, Ali̇ Ulaş

Turkish Journal of Veterinary & Animal Sciences

On 17 October 2012, an abnormally pigmented specimen of the surmullet Mullus surmuletus L. with a standard length of 164 mm was caught off the coast of Urla in İzmir Bay (Aegean Sea). This is the first report of abnormal pigmentation of the surmullet in the Mediterranean Basin. The sample fish had a patterned blue color on the back and its flanks were silvery. This kind of malpigmentation has not been observed in any mullids up to now.

Glutaredoxin-2 Is Required To Control Proton Leak Through Uncoupling Protein-3, Ryan J. Mailloux, Jian Ying Xuan, Brittany Beauchamp, Marjorie F. Lou, Mary-Ellen Harper

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutathionylation has emerged as a key modification required for controlling protein function in response to changes in cell redox status. Recently, we showed that the glutathionylation state of uncoupling protein-3 (UCP3) modulates the leak of protons back into the mitochondrial matrix, thus controlling reactive oxygen species production. However, whether or not UCP3 glutathionylation is mediated enzymatically has remained unknown because previous work relied on the use of pharmacological agents, such as diamide, to alter the UCP3 glutathionylation state. Here, we demonstrate that glutaredoxin-2 (Grx2), a matrix oxidoreductase, is required to glutathionylate and inhibit UCP3. Analysis of bioenergetics in skeletal muscle …

Omega-3 Fatty Acid Supplementation In The Management Of Chronic Lower Airway Inflammatory Conditions In Horses, Nora Nogradi

Open Access Theses

Chronic lower airway inflammatory diseases, such as recurrent airway obstruction (RAO) and inflammatory airway disease (IAD) are common in horses. RAO and IAD present many similarities with asthma in people. Based on studies in human asthma, omega - 3 polyunsaturated fatty acid (PUFA) supplementation has been proposed to improve clinical signs in horses with chronic lower airway inflammatory disease. The purpose of this study was to evaluate the clinical efficacy of an omega-3 PUFA containing equine feed supplement (Aleira^TM) in horses with chronic lower airway disease. The study consisted of two separate experiments. First, a pilot study was …

A Dysfunctional Tricarboxylic Acid Cycle Enhances Fitness Of Staphylococcus Epidermidis During Β-Lactam Stress, Vinai Chittenzham Thomas, Lauren Kinkhead, Ashley Janssen, Carolyn Schaeffer, Keith Woods, Jill Lindgren, Johnathan Peaster, Sujata Chaudhari, Marat Sadykov, Joselyn Jones, Sameh Mohamadi Abdelghani, Matthew Zimmerman, Kenneth W. Bayles, Greg Somerville, Paul Fey

School of Veterinary and Biomedical Sciences: Faculty Publications

A recent controversial hypothesis suggested that the bactericidal action of antibiotics is due to the generation of endogenous reactive oxygen species (ROS), a process requiring the citric acid cycle (tricarboxylic acid [TCA] cycle). To test this hypothesis, we assessed the ability of oxacillin to induce ROS production and cell death in Staphylococcus epidermidis strain 1457 and an isogenic citric acid cycle mutant. Our results confirm a contributory role for TCA-dependent ROS in enhancing susceptibility of S. epidermidis toward β-lactam antibiotics and also revealed a propensity for clinical isolates to accumulate TCA cycle dysfunctions presumably as a way to tolerate these …

Manipulation Of Cellular Processing Bodies And Their Constituents By Viruses, Asit K. Pattnaik, Phat X. Dinh

School of Veterinary and Biomedical Sciences: Faculty Publications

The processing bodies (PBs) are a form of cytoplasmic aggregates that house the cellular RNA decay machinery as well as many RNA-binding proteins and mRNAs. The PBs are constitutively present in eukaryotic cells and are involved in maintaining cellular homeostasis by regulating RNA metabolism, cell signaling, and survival. Virus infections result in modification of the PBs and their constituents. Many viruses induce compositionally altered PBs, while many others use specific components of the PBs for their replication. PB constituents are also known to restrict virus replication by a variety of mechanisms. Further, continuing studies in this rapidly emerging field of …

Characterization Of A Serologic Marker Candidate For Development Of A Live-Attenuated Diva Vaccine Against Porcine Reproductive And Respiratory Syndrome Virus, Hiep Vu, Byungjoon Kwon, Marcelo De Lima, Asit K. Pattnaik, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

DIVA (differentiating infected from vaccinated animals) vaccines have proven extremely useful for control and eradication of infectious diseases in livestock. We describe here the characterization of a serologic marker epitope, so-called epitope-M201, which can be a potential target for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). Epitope-M201 is located at the carboxyl terminus (residues 161-174) of the viral M protein. The epitope is highly immunodominant and well-conserved among type-II PRRSV isolates. Rabbit polyclonal antibodies prepared against this epitope are non-neutralizing; thus, the epitope does not seem to contribute to the protective immunity against …

Trex1 Regulates Lysosomal Biogenesis And Interferon-Independent Activation Of Antiviral Genes, Maroof Hasan, James Koch, Dinesh Rakheja, Asit K. Pattnaik, James Brugarolas, Igor Dozmorov, Beth Levine, Edward K. Wakeland, Min Ae Lee-Kirsch, Nan Yan

School of Veterinary and Biomedical Sciences: Faculty Publications

Innate immune sensing of viral nucleic acids triggers type I interferon (IFN) production, which activates interferon-stimulated genes (ISGs) and directs a multifaceted antiviral response. ISGs can also be activated through IFN-independent pathways, although the precise mechanisms remain elusive. Here we found that the cytosolic exonuclease Trex1 regulates the activation of a subset of ISGs independently of IFN. Both Trex1−/− mouse and TREX1-mutant human cells express high levels of antiviral genes and are refractory to viral infections. The IFN-independent activation of antiviral genes in Trex1−/− cells requires STING, TBK1 and IRF3 and IRF7. We also found that Trex1-deficient cells display expanded …

Influence Of Therapeutic Ceftiofur Treatments Of Feedlot Cattle On Fecal And Hide Prevalences Of Commensal Escherichia Coli Resistant To Expanded-Spectrum Cephalosporins, And Molecular Characterization Of Resistant Isolates, John W. Schmidt, Dee Griffin, Larry A. Kuehn, Dayna M. Brichta-Harhay

School of Veterinary and Biomedical Sciences: Faculty Publications

In the United States, the bla_CMY-2 gene contained within incompatibility type A/C (IncA/C) plasmids is frequently identified in extended-spectrum-cephalosporin-resistant (ESC^r) Escherichia coli strains from both human and cattle sources. Concerns have been raised that therapeutic use of ceftiofur in cattle may increase the prevalence of ESC^r E. coli. We report that herd ESC^r E. coli fecal and hide prevalences throughout the residency of cattle at a feedlot, including during the period of greatest ceftiofur use at the feedlot, were either not significantly different (P≥0.05) or significantly less (Pr E. coli shedding that follows ceftiofur …

Complete Closed Genome Sequences Of Mannheimia Haemolytica Serotypes A1 And A6, Isolated From Cattle, Gregory P. Harhay, Sergey Koren, Adam M. Phillippy, D. Scott Mcvey, Jennifer Kuszak, Michael L. Clawson, Dayna M. Harhay, Michael P. Heaton, Carol G. Chitko-Mckown, Timothy P. L. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

Mannheimia haemolytica is a respiratory pathogen affecting cattle and related ruminants worldwide. M. haemolytica is commonly associated with bovine respiratory disease complex (BRDC), a polymicrobial multifactorial disease.Wepresent the first two complete closed genome sequences of this species, determined using an automated assembly pipeline requiring no manual finishing.

Deep Sequencing And Genome-Wide Analysis Reveals The Expansion Of Microrna Genes In The Gall Midge Mayetiola Destructor, Chitvan Khajuria, Christie E. Williams, Mustapha El Bouhssini, R. Jeff Whitworth, Stephen Richards, Jeffrey J. Stuart, Ming-Shun Chen

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in regulating post transcriptional gene expression. Gall midges encompass a large group of insects that are of economic importance and also possess fascinating biological traits. The gall midge Mayetiola destructor, commonly known as the Hessian fly, is a destructive pest of wheat and model organism for studying gall midge biology and insect – host plant interactions.

Results: In this study, we systematically analyzed miRNAs from the Hessian fly. Deep-sequencing a Hessian fly larval transcriptome led to the identification of 89 miRNA species that are either identical or very …

Development And Evaluation Of A Replicon Particle Vaccine Expressing The E2 Glycoprotein Of Bovine Viral Diarrhea Virus (Bvdv) In Cattle, John Dustin Loy, Jill Gander, Mark Mogler, Ryan Vander Veen, Julia Ridpath, Delbert Hank Harris, Kurt Kamrud

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Bovine viral diarrhea virus is one of the most significant and costly viral pathogens of cattle worldwide. Alphavirus-derived replicon particles have been shown to be safe and highly effective vaccine vectors against a variety of human and veterinary pathogens. Replicon particles are non-propagating, DIVA compatible, and can induce both humoral and cell mediated immune responses. This is the first experiment to demonstrate that Alphavirus-based replicon particles can be utilized in a standard prime/boost vaccination strategy in calves against a commercially significant bovine pathogen.

Findings: Replicon particles that express bovine viral diarrhea virus sub-genotype 1b E2 glycoprotein were …

Mutation From Arginine To Lysine At The Position 189 Of Hemagglutinin Contributes To The Antigenic Drift In H3n2 Swine Influenza Viruses, Jianqiang Ye, Yifei Xu, Jillian Harris, Hailiang Sun, Andrew S. Bowman, Fred L. Cunningham, Carol Cardona, Kyoungjin J. Yoon, Richard D. Slemons, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Two distinct antigenic clusters were previously identified among the H3N2 swine influenza A viruses (IAVs) and were designated H3N2SIV-alpha and H3N2SIV-beta (Feng et al., 2013. Journal of Virology 87(13), 7655–7667). A consistent mutation was observed at the position 189 of hemagglutinin (R189K) between H3N2SIV-alpha and H3N2SIV-beta fair isolates. To evaluate the contribution of R189K mutation to the antigenic drift from H3N2SIV-alpha to H3N2SIV-beta, four reassortant viruses with189R or189K were generated. The antigenic cartography demonstrated that the R189K mutation in the hemagglutinin of H3N2IAV contributed to the antigenicdrift, separating these viruses into H3N2SIV-alpha to H3N2SIV- beta. This R189K mutation was also …

Herpes Simplex Virus Type 1 Glycoprotein Gm And The Membrane Associated Protein Ul11 Are Required For Virus-Induced Cell Fusion And Efficient Virus Entry, In Joong Kim

LSU Doctoral Dissertations

HSV-1 facilitates virus entry into cells and cell-to-cell spread by mediating fusion of the viral envelope with cellular membranes and fusion of adjacent cellular membranes. Although virus strains isolated from herpetic lesions cause limited cell fusion in cell culture, clinical herpetic lesions typically contain large syncytia, underscoring the importance of cell-to-cell fusion in virus spread in infected tissues. Certain mutations in gB, gK, UL20 and other viral genes, drastically enhance virus-induced cell fusion in vitro and in vivo. Recent work has suggested that gB is the sole fusogenic glycoprotein, which is regulated by interactions with viral glycoproteins gD, gH/gL, gK, …

Characterization, Prevalence, And Risk Factors Of Spontaneous And Experimental Atherosclerosis And Vascular Imaging In Psittaciformes, Hugues Beaufrère

LSU Doctoral Dissertations

Atherosclerosis is a degenerative and inflammatory vascular disease characterized by the accumulation of inflammatory cells, lipids, calcium, and formation of large fibrofatty lesions within the intima of arteries resulting in the disorganization of the arterial wall and stenosis of the lumen. Despite the importance of atherosclerosis in psittacine cardiology, there are few pathologic, clinical, and experimental investigations in psittaciformes. In order to expand the knowledge on psittacine atherosclerosis and provide fundamental observational information for future research, a series of studies was conducted on psittaciformes: 1) psittacine atherosclerotic lesions were characterized and categorized based on histopathology, histochemistry, transmission (TEM), and scanning …

General Reproductive Properties In Pigs, İrfan Tur

Turkish Journal of Veterinary & Animal Sciences

Pig breeding is usually undertaken for fat and meat production, but different sectors (cosmetics industry, pharmaceutical industry, medical sector, etc.) can also use pigs for alternate purposes. Pork is evaluated based on the status of an amended law regarding butchers' meat in Turkey. There have been 25 pig farms registered in Turkey since 7 July 2006 in the framework of compliance with the European Union. On average, a sow has 2.3 farrowings per year, producing 9.5 to 12.5 piglets per litter. It is important that high proportions of selected replacement gilts reach puberty at an early age in order to …

Prevention Of Embryonic Death Using Different Hormonal Treatments In Ewes, Mehmet Bozkurt Ataman, Mehmet Aköz, Mustafa Kemal Saribay, Hüseyi̇n Erdem, Mustafa Numan Bucak

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to evaluate different treatment protocols to prevent embryonic death in ewes. A total of 180 Akkaraman crossbred ewes and 10 healthy rams were used as material. The ewes were divided into 3 equal groups, with each of the 3 groups then separated into 3 subgroups. Ewes in estrus, determined with teaser rams, were exposed to mating. Three different treatment protocols of gonadotropin-releasing hormone (GnRH) analog (buserelin, intramuscularly) at a dose of 20 µg, vaginal sponges containing 30 mg fluorogestone acetate (FGA), and saline at a dose of 1 mL (control, intramuscularly) were applied on …

Microsatellite Analysis For Parentage Testing Of The Arabian Horse Breed From Syria, Anas Mahmoud Khanshour, Eleanore Kathleen Conant, Rytis Juras, Ernest Gus Cothran

Turkish Journal of Veterinary & Animal Sciences

Parentage testing is very important for genetically determining the accuracy of the pedigree of domestic animals. The Arabian horse population in Syria might be expected to have a high level of homozygosity because the traditional method of breeding results in such a small population. For this reason, using a set of highly polymorphic markers such as microsatellites is required for parentage testing. The purpose of this study was to analyze the usefulness of 16 STR markers for routine parentage testing in 94 samples from registered and nonregistered horses collected from Syria. The mean number of alleles was 5.69 per locus …

Effect Of Different Transport Temperatures On In Vitro Maturation Of Oocytes Collected From Frozen-Thawed Sheep Ovaries, Özen Banu Özdaş, Alper Baran, Muzaffer Taş, Ümüt Ci̇ri̇t, Kamber Demi̇r, Süleyman Bacinoğlu, Serhat Pabuccuoğlu, Kemal Ak

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of 2 different transport temperatures on the in vitro maturation of oocytes collected from frozen-thawed sheep ovaries. Sheep ovaries were transferred into saline at temperatures of 4 °C and 32 °C. After the 2 experimental groups (A: fresh cortex, B: frozen-thawed cortex) were formed, each group was divided into 2 subgroups (group A1: 4 °C, group A2: 32 °C [control]; group B1: 4 °C, group B2: 32 °C). The cortexes were dissected into slices 1-3 mm thick and pieces of 0.5 cm2. For groups B1 and B2, 1-2 cortex pieces …

Effect Of Oocyte Quality And Activation Protocols On Bovine Embryo Development Following Intracytoplasmic Sperm Injection, Ömer Korkmaz, Şükrü Küplülü, Yüksel Ağca, İbrahi̇m Mert Polat

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to investigate the effects of oocyte quality and activation protocols on the in vitro developmental competence of bovine embryos after intracytoplasmic sperm injection (ICSI). Bovine oocytes were grouped as being of excellent, good, and poor quality. All of the oocytes were activated using a calcium ionophore only, ethanol only, and 6-dimethylaminopurine (6-DMAP) following calcium ionophore. For the excellent quality oocytes, cleavage rates after ICSI were 70% in the Ca ionophore + 6-DMAP activation group, 47% in the ethanol activation group, 41.82% in the Ca ionophore group, and 30% in the nontreated control group. Cleavage …

Seroepidemiology Of Canine Brucellosis In Beijing, China, Fu Xiang, Zhaofei Xia, Qingming Wu, Yanyun Chen, Jinhau Yu, Jianging Wan

Turkish Journal of Veterinary & Animal Sciences

A seroepidemiological survey of canine brucellosis was carried out in Beijing, China. Serum samples were taken from dogs admitted to the Beijing Companion Animal Hospital for immigration and emigration inspection, including dogs from 4 breeding farms and stray dogs (n = 1200, 14 districts), between January 2008 and April 2009. All of the sera were first tested and preselected by Rose Bengal plate agglutination test (RBAT), and the positive samples by RBAT were determined by tube agglutination test (TAT) again. Of the 1200 dogs studied, 60 (5%) were positive by RBAT, 21 (51.7%) of which were confirmed by TAT. The …

Changes In The Bioactive Protein Concentrations In The Bovine Colostrum Of Jersey And Polish Holstein–Friesian Cows, Monika Sobczuk-Szul, Zofia Wielgosz-Groth, Marek Wronski, Arkadiusz Rzemieniewski

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine changes in the bioactive protein concentrations in the bovine colostrum of Jersey and Polish Holstein-Friesian (PHF) cows. Double colostrum samples were collected from 16 Jersey and PHF cows, during 8 successive milking runs after calving. A total of 256 colostrum samples were analyzed to determine the pH, density, percentage share, and content of selected bioactive proteins, including the percentage share of casein fractions (\alpha_s-, \beta-, \gamma-, and \kappa-) in the total casein, and the concentrations of \alpha-lactalbumin (\alpha-LA), \beta-lactoglobulin (\beta-LG), lactoferrin (LF), interleukin-1\beta, interleukin-6, and tumor necrosis factor-alpha. The concentrations of the …

A Retrospective Study Of Tetralogy Of Fallot In Dogs, Urszula Paslawska, Agnieszka Noszczyk-Nowak, Alicja Cepiel, Maciej Staszczyk, Adrian Janiszewski

Turkish Journal of Veterinary & Animal Sciences

Tetralogy of Fallot is a rare congenital heart defect; however, it is the most common cyanotic heart malformation in humans and animals. It consists of a ventricular septal defect, pulmonic stenosis, displacement of the aortic root, and hypertrophy of the right ventricle. At the Department of Internal Medicine Clinic of Diseases of Horses, Dogs, and Cats at the Wroclaw University of Environmental and Life Sciences, patients with Fallot syndrome constituted 4.44% of all patients with congenital heart diseases. There was no breed predisposition found. The most common clinical signs were exercise intolerance, dyspnea, and cyanosis of the mucous membranes, but …

The Effect Of Selenized Yeast Supplementation On Some Performance Parameters In Sheep, Bogumila Pilarczyk, Agnieszka Tomza-Marciniak, Zbigniew Dobrzanski, Malgorzata Szewczuk, Tomasz Stankiewicz, Dariusz Gaczarzewicz, Wlodzimierz Lachowski

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the levels of serum selenium in coarse-haired Pomeranian sheep and to evaluate the effect of the oral supplementation of selenized yeast on selected performance parameters in such sheep. The sheep were divided into 2 groups, with 53 ewes forming the experimental group and 34 ewes forming the control group. The concentration of serum selenium in all 87 sheep from the 2 groups was analyzed. The sheep in the experimental group received selenized yeast for the 3 days before and 3 days after mating (1 g preparation/animal daily, with 1 g of preparation …

Whole-Blood Thrombelastography Using Calcium Chloride Activation In Healthy Cats, Ramazan Çöl, Alicia Montgomery, Maria Cristina Iazbik, Ashley Defelice, Poulo Vilar Saavedra, Guillermo Couto

Turkish Journal of Veterinary & Animal Sciences

Thrombelastography (TEG, Haemoscope Corp., Niles, IL, USA) allows for a global evaluation of the hemostatic system; while conventional coagulation tests typically evaluate only one part of the coagulation system, TEG simultaneously examines the interaction between platelets, clotting factors, the fibrinolytic system, and clot retraction mechanisms. Since its development, TEG has been widely employed in human clinical medicine and research, but has only recently gained popularity in veterinary medicine. The purpose of this study was to establish TEG reference ranges in healthy cats using the citrated native technique. In this study, 31 clinically healthy cats were evaluated. We sampled healthy adult …

A Pilot Study On The Epidemiological Status Of Equine Infectious Anaemia, Equine Viral Arteritis, Glanders, And Dourine In Turkey, Maria Luisa Marenzoni, Vincenzo Cuteri, Francesca De Parri, Maria Luisa Danzetta, Zeki̇ Yilmaz, Cagla Parkan Yaramis, Engi̇n Kennerman, Mehmet Erman Or, Silvia Marchi, Cristina Casciari, Gian Mario De Mia, Carlo Valente, Silva Costarelli

Turkish Journal of Veterinary & Animal Sciences

A serological investigation of equine infectious anaemia (EIA), equine viral arteritis (EAV), glanders, and dourine was conducted on the sera of 346 Turkish horses using a combination of tests in series (ELISA and agar gel immunodiffusion) for EIA, virus neutralisation for EAV, and complement fixation (CF) for glanders and dourine. Forty-nine sera showed anticomplementary reactions and were not assessable in the CF test for glanders and dourine. No positive samples were detected for EIA, dourine, and glanders. Fifty-seven sera were positive for EAV. A systematic review of the distribution of these diseases in Turkey was conducted to describe their epidemiological …

Determination Of Newcastle Disease Virus Among Wild Bird Populations In Lake Van Basin, Turkey, Banur Boynukara, Ti̇mur Gülhan, Fethi̇ye Çöven, İlhami̇ Ki̇zi̇roğlu, Ati̇lla Durmuş

Turkish Journal of Veterinary & Animal Sciences

In this study, 540 feces samples taken from a total of 22 bird species, including transit migrant, winter visitor, migratory, and resident bird species, in Lake Van basin were examined by real-time polymerase chain reaction (RT-PCR) with respect to the Newcastle disease virus (NDV). All of the feces samples were cultured in specific pathogen-free embryonated chicken eggs (ECEs) for NDV isolation. The NDV isolates were analyzed for virulent fusion (F) protein by RT reverse transcription PCR (RRT-PCR). Of the 540 examined feces samples, 28 (5.18%) were found to be positive by RT-PCR. The same samples were cultured in ECEs and, …

Susceptibility Of Prototheca Zopfii Strains Isolated From Cows With Mastitis To Chlorhexidine And Iodine, Henryk Krukowski, Andrzej Lisowski, Bozena Nowakowicz-Debek, Lukasz Wlazlo

Turkish Journal of Veterinary & Animal Sciences

Bovine mastitis caused by Prototheca spp. can assume high significance because of economic losses and the potential risk to public health. The aim of our study was to evaluate the effect of chlorhexidine and iodine on Prototheca zopfii strains isolated from bovine milk. For this study, 15 Prototheca zopfii isolates previously obtained from cows with clinical and subclinical mastitis in Poland were used. The in vitro susceptibility tests to chlorhexidine and iodine of the strains of Prototheca zopfii for the determination of their minimal microbicidal concentrations (MMCs) were performed using the tube dilution method in Sabouraud dextrose broth and the …