Veterinary Medicine Commons^™

Performance Of Broilers Fed Raw Or Fermented And Redried Wheat, Barley, And Oat Grains, Sulhatti̇n Yaşar, Muhammed Saci̇d Gök, Yavuz Gürbüz

Turkish Journal of Veterinary & Animal Sciences

The efficacy of diets containing 40%-50% wheat, barley, or oat grains, fermented by a solid-state fermentation process, on bird performance, carcass yield, digestive organ development, and intestinal viscosity was tested. Compared with broiler chicks fed unfermented grains, those fed fermented oats had increased feed intake, and treatment with fermented wheat and barley improved the feed conversion ratio at days 21 and 42. Treatment with fermented barley and oats resulted in increased body weight at day 42. Treatment with fermented wheat and oats increased the percentage of carcass yield. Birds fed unfermented grains had highly viscous digesta, not seen among birds …

The Efficacy Of Kaolin Clay In Reducing The Duration And Severity Of `Heat' Diarrhea In Foals, Magdalena Pieszka, Jaroslaw Luszczynski, Magdalena Hedrzak, Katerina Goncharova, Stefan G. Pierzynowski

Turkish Journal of Veterinary & Animal Sciences

'Heat' diarrhea in foals is an onerous but not life-threatening ailment, which indicates that it may be of osmotic origin. This was confirmed by a successful attempt, presented in this paper, to alleviate the severity and duration of foal heat diarrhea with the use of a typical absorbent, kaolin clay, as a feed additive, usually applied in feed production as an anticaking agent. Based on the present results, it can be concluded that treatment of foals maintained on different stud farms with a kaolin paste reduced the duration of heat diarrhea and alleviated its severity (P < 0.05 and P = 0.001). The observed action of kaolin clay, an excellent absorbent, suggests that the so-called heat diarrhea can be caused by disturbances in the intestinal osmotic balance at this specific age of foals. The use of this preparation can reduce the risk of secondary pathological viral or bacterial diarrhea and the requirement for veterinary intervention in foal management. It can also shorten the care period and increase foal well-being, essential for the normal development of a young horse. In addition, prophylactic application of an antidiarrheal preparation based on an industrial by-product, i.e. A cheap component, will reduce the costs of foal care.

Mycoflora And Chemotype Characterization Of Fusarium Graminearum Isolatesobtained From Corn And Barley Used As Feedstuffs In The Northeast Of Iran, Abdollah Salehi, Roya Rezaeian Doloei

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the mycoflora and chemotyping of Fusarium graminearum isolates in corn and barley used in industrial dairy farms in Khorasan-Razavi Province, northeastern Iran. For this purpose, a total of 128 corn and barley samples were collected from 16 different large industrial dairy farms. Mean moisture of the samples collected in different seasons of the year was significantly different (P < 0.05). The most prevalent fungi isolated from corn samples were Penicillium spp. (90.62%) and Aspergillus spp. (82.81%), and that of barley samples was Penicillium spp. (89.06%). Frequency of Fusarium spp. İn corn and barley samples was 85.93% and 26.56%, respectively. All isolates (100%) were confirmed as Fusarium using an ITS primer. Eight (11.11%) out of 72 Fusarium spp. İsolates were confirmed as F. Graminearum using species-specific primers. Five out of 8 F. Graminearum isolates were positive in terms of the presence of the trichothecene-producing gene using a Tri5-specific primer. Three and 2 out of 5 toxin-producing F. Graminearum isolates were positive using Tri13DON- and Tri13NIV-specific primers, respectively. In conclusion, the presence of toxigenic Fusarium in corn and barley samples used in feedstuffs in Iran is a potential risk to animal and human health.

Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial

Turkish Journal of Veterinary & Animal Sciences

The activity of enzymes that digest carbohydrates at various points in the gastrointestinal tract of sheep was examined. In addition, weight and contents of digestive tract segments were examined. The experiment was performed on six adult ewes fed an 85% hay diet. The morphometric measurements revealed that the alimentary tract constituted 32% of the sheep's body mass and wet rumen contents constituted 70% of wet mass of the total digestive tract content. The protozoal population in the rumen varied from 37.2 × 104 to 54.0 × 104/mL rumen fluid. In the examined samples, ciliates were identified as belonging to the …

Appraisal Of The Impact Of Aluminosilicate Use On The Health And Performance Of Poultry, Ali Gilani, Hassan Kermanshahi, Abolghasem Golian, Saeed Seifi

Turkish Journal of Veterinary & Animal Sciences

Aluminosilicates are feed additives used for multiple purposes in the poultry industry. Bentonites, zeolites, and kaolins are types of aluminosilicate compounds that appear in the marketplace due to their capabilities as mycotoxin adsorbents and other qualifications. While there are some differences among their efficacies, anecdotal evidence confirms that these additives ameliorate aflatoxicosis and manure ammonia emission, and improve the durability of pelleted diets. Furthermore, these supplements are safe at 0.2%-1% inclusion levels in the commercial diet of broilers and layers and did not have any adverse effects on productive indices, and even promoted birds' performance. Nevertheless, their upper levels, especially …

Effect Of Dietary Orange Peel Extract On Physiological, Biochemical, And Metabolic Responses Of Japanese Quail Reared Under Low Ambient Temperature, Mehmet Çi̇ftçi̇, Ülkü Gülci̇han Şi̇mşek, Bestami̇ Dalkiliç, Mehmet Ali̇ Azman, Ökkeş Yilmaz, Seda İflazoğlu Mutlu, Mehtap Özçeli̇k, Yasi̇n Baykalir, Fadi̇me Tonbak, Muammer Bahşi̇

Turkish Journal of Veterinary & Animal Sciences

The effects of dietary orange peel extract (OPE) supplementation on growth performance, carcass traits, serum biochemical parameters, antioxidant status of tissues, and fatty acid composition of breast meat in Japanese quails reared under low ambient temperature were investigated in the present study. A total of 108 quails of 15 days old were assigned to 3 groups with 3 replicates. Animals were fed with a corn/soy-based standard diet in the control group and a standard diet supplemented with either 100 or 200 ppm of OPE in the experimental groups (control, OPE-100, and OPE-200 groups, respectively). Room temperature was gradually lowered at …

Overall Prevalence Of Feline Overweight/Obesity In Japan As Determined Froma Cross-Sectional Sample Pool Of Healthy Veterinary Clinic-Visiting Cats In Japan, Nobuko Mori, Eiji Iwasaki, Yuki Okada, Koh Kawasumi, Toshiro Arai

Turkish Journal of Veterinary & Animal Sciences

Many veterinarians feel that feline obesity is increasing in prevalence within Japan, but are unsure as to what real rate of obesity prevalence exists currently. The objectives of this study are twofold. First, we sought to determine the ratio of overweight/obese cats, as determined via body condition score (BCS), based on a cross-sectional pool of healthy cats. Second, using a uniform method of biochemical measurement for cat plasma collected all over Japan, we sought to construct cross-sectional working reference ranges of plasma metabolites using age, BCS, and sex as classifying factors. Overall, the rate of overweight and obese cats (BCS …

The Effects Of Probiotics, Organic Acid, And A Medicinal Plant On The Immune Systemand Gastrointestinal Microflora In Broilers Challenged With Campylobacter Jejuni, Hossein Ebrahimi, Shaban Rahimi, Pejvak Khaki, Jesse L. Grimes, Sophia Kathariou

Turkish Journal of Veterinary & Animal Sciences

Campylobacter jejuni, a zoonotic bacterial pathogen with worldwide distribution, infects about 400 million humans in the world annually. In order to reduce C. Jejuni colonization in the gastrointestinal tracts of broilers and make chickens less susceptible to colonization, four commercial products based on organic acid, probiotics, and medicinal plants were used. In this experiment, 210 one-day-old male broiler chicks (Ross 308) were assigned to 7 treatment groups randomly with 3 replications and 10 birds in each pen. Birds were challenged on day 21 by 1 mL of 6 × 107 CFU/mL C. Jejuni live suspension and samples were collected on …

Application Of Herbs And Propolis In Rabbits With Chronic Diarrhea, Robert Kupczynski, Tomasz Piasecki, Michal Bednarski, Kinga Spitalniak, Anna Budny-Walczak

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effect of ethanolic extract of propolis (EEP) and an herbal mixture (Rumex crispus, Potentilla anserina, and Polygonum aviculare) on diarrhea course, hematological and biochemical parameters, and acid-base balance of rabbits. Twenty-four meat rabbits (Hyplus) were divided into four groups of eight animals. The rabbits were randomly divided into the following groups: HR - healthy rabbits, DR - rabbits with diarrhea symptoms, H - treated with 10% herbal extract, and EEP - treated with 10% ethanolic extract of propolis at 4 mL/L. The extracts were supplemented for 10 days into the drinking …

Influence Of Flaxseed Combined With Thyme, Rosemary, And Sage Leaves Asfodder Additives On Antioxidant Status In The Liver Of Japanese Quail, Radoslaw Drozd, Artur Rybarczyk, Agata Wasak, Malgorzata Jakubowska, Kinga Rybak, Magdalena Skolmowska

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the influence of a combination of plant supplements with antioxidant properties on the total antioxidant status measured by FRAP and the activity of selected antioxidant enzymes in the livers of Japanese quails. The birds were given feed with the 4% addition of flaxseed combined with a 1% admixture of dried thyme, rosemary, or sage leaves, respectively. The results showed an increased value of the antioxidant potential in the livers of quails fed with a diet that contained the combination of flaxseed and thyme, compared to the control diet and the diet that …

Evaluation Of Immunotherapeutic Potential Of Ocimum Sanctumin Bovine Subclinical Mastitis, Tawheed Ahmad Shafi, Baljinder Kumar Bansal, Dhiraj Kumar Gupta, Shashi Nayyar

Turkish Journal of Veterinary & Animal Sciences

Mastitis, the inflammation of mammary glands, is caused by a wide range of contagious and environmental microbes, whose clearance from the udder is generally dependent on the use of antibiotics. However, antibiotic therapy may result in development of drug-resistant microbes and depress the activity of polymorphonuclear cells. The present study was therefore undertaken to explore the immunotherapeutic potential of an herb, Ocimum sanctum, in mastitis in dairy cows. The study involved twenty HF × Sahiwal lactating dairy cows, each identified with at least one specific subclinical mastitis quarter as per International Dairy Federation criteria. The cows were divided into two …

Animal Mourning: Précis Of How Animals Grieve (King 2013), Barbara J. King

Animal Sentience

Abstract: When an animal dies, that individual’s mate, relatives, or friends may express grief. Changes in the survivor’s patterns of social behavior, eating, sleeping, and/or of expression of affect are the key criteria for defining grief. Based on this understanding of grief, it is not only big-brained mammals like elephants, apes, and cetaceans who can be said to mourn, but also a wide variety of other animals, including domestic companions like cats, dogs, and rabbits; horses and farm animals; and some birds. With keen attention placed on seeking where grief is found to occur and where it is absent …

The Science Of Sentience Is Reshaping How We Think About Animals, Debra Durham

Animal Sentience

Broom’s Sentience and Animal Welfare (2014) provides its readers with an excellent overview of the evolving role of sentience in our understanding animal welfare as well as insights into why sentience matters for practice and policy.

Breaking The Silence: The Veterinarian’S Duty To Report, Martine Lachance

Animal Sentience

Animals, like children and disabled elders, are not only the subjects of abuse, but they are unable to report and protect themselves from it. Veterinarians, like human physicians, are often the ones to become aware of the abuse and the only ones in a position to report it when their human clients are unwilling to do so. This creates a conflict between professional confidentiality to the client and the duty to protect the victim and facilitate prosecution when the law has been broken. I accordingly recommend that veterinarian associations make reporting of abuse mandatory.

Tissue Restricted Splice Junctions Originate Not Only From Tissue-Specific Gene Loci, But Gene Loci With A Broad Pattern Of Expression, Matthew S. Hestand, Zheng Zeng, Stephen J. Coleman, Jinze Liu, James N. Macleod

Maxwell H. Gluck Equine Research Center Faculty Publications

Cellular mechanisms that achieve protein diversity in eukaryotes are multifaceted, including transcriptional components such as RNA splicing. Through alternative splicing, a single protein-coding gene can generate multiple mRNA transcripts and protein isoforms, some of which are tissue-specific. We have conducted qualitative and quantitative analyses of the Bodymap 2.0 messenger RNA-sequencing data from 16 human tissue samples and identified 209,363 splice junctions. Of these, 22,231 (10.6%) were not previously annotated and 21,650 (10.3%) were expressed in a tissue-restricted pattern. Tissue-restricted alternative splicing was found to be widespread, with approximately 65% of expressed multi-exon genes containing at least one tissue-specific splice junction. …

Summer-Long Grazing Of High Vs. Low Endophyte (Neotyphodium Coenophialum)-Infected Tall Fescue By Growing Beef Steers Results In Distinct Temporal Blood Analyte Response Patterns, With Poor Correlation To Serum Prolactin Levels, Joshua J. Jackson, Merlin D. Lindemann, James A. Boling, James C. Matthews

Animal and Food Sciences Faculty Publications

Previously, we reported the effects of fescue toxicosis on developing Angus-cross steer growth, carcass, hepatic mRNA, and protein expression profiles of selected serum proteins, and blood clinical and chemical profiles, after summer-long grazing (85 days) of high endophyte (HE)- vs. low endophyte (LE)-infected fescue pastures. We now report the temporal development of acute, intermediate, and chronic responses of biochemical and clinical blood analytes determined at specified time intervals (period 1, day 0–36; period 2, day 37–58; and period 3, day 59–85). Throughout the trial, the alkaloid concentrations of the HE forage was consistently 19–25 times greater (P ≤ 0.002) …

Autonomic Nervous System Reactivity In A Free-Ranging Mammal: Effects Of Dominance Rank And Personality, Elodie F. Briefer, James A. Oxley, Alan G. Mcelligott

Ethology Collection

Modulation of the autonomic nervous system (ANS) activity allows animals to effectively respond to internal and external stimuli in everyday challenges via changes in, for example, heart and respiration rate. Various factors, ranging from social such as dominance rank to internal such as personality or affective states can impact animal physiology. Our knowledge of the combinatory effects of social and internal factors on ANS basal activity and reactivity, and of the importance that each factor has in determining physiological parameters, is limited, particularly in nonhuman, free-ranging animals. In this study, we tested the effects of dominance rank and personality (assessed …

Prevalence Of Tick-Borne Pathogens In Small Mammals And White-Tailed Deer In Southeast Nebraska, Tim M. Hotaling

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The prevalence of tick-borne diseases has been increasing in the United States for the past couple decades. Studies have been conducted throughout the US identifying tick-borne disease pathogens as well as their hosts and prevalence. Research was conducted in Nebraska to determine the presence of some tick-borne disease pathogens, their vectors, and their hosts, with emphasis made on Borrelia spp., Rickettsia rickettsii, and Ehrlichia chaffeensis.

Small rodents in southeast Nebraska were trapped and sampled at eight study sites using live capture traps. Captured rodents were assessed for active parasitism by ticks which were collected and placed in alcohol. …

Characterization Of Internal Transcribed Spacer Of Nuclear Ribosomal Dna Of Trichuris Ovis From Goat In Hunan Province, China, Fen Li, Yi Liu, Wen-Cheng Chen, Tao Hu, Liu-Chun Duan, Wei Liu, Tian-Yin Cheng

The Thai Journal of Veterinary Medicine

Trichuris ovis parasitizes the caeca and colon of hosts, causing trichuriasis. Despite this parasite being of animal health significance, causing considerable socio-economic losses globally, little is known of the molecular characteristics of T. ovis. In the present study, the entire first and second internal transcribed spacer (ITS-1 and ITS-2) regions of nuclear ribosomal DNA (rDNA) of T. ovis from goats in Hunan province, China were amplified by polymerase chain reaction (PCR), and the representative amplicons were subjected to sequencing in order to estimate sequence variation. The ITS rDNA sequences for the T. ovis samples were 1289-1303 bp in length. Sequence …

Effect Of Serum Il-1beta Of Pcso-524 And Firocoxib In Dogs Undergoing Medial Patellar Luxation Repair, Sararat Kongwut, Kumpanart Soontornvipart, Meena Sarikaphuti, Pussadee Makoom, Korakot Nganvongpanit

The Thai Journal of Veterinary Medicine

Thirty client-owned Pomeranians, between one and seven years old that had grade-three medial patellar luxation, were enrolled in this experiment. The dogs did not have other diseases. All the dogs received the same physical and clinical orthopaedic examinations, as well as the peak vertical force gait analysis, radiographic examination for assessment of bone deformities. Then, blood was collected from each dog for evaluation of complete blood counts (CBCs), blood chemistry and biomarker assays (IL-1β) before surgery. After surgery to correct their medial patellar luxations, they were divided into three groups by random sampling. The first group received only Firocoxib (5 …

The Neurobiology Of Circadian Rhythms, Patricia J. Sollars, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

Daily rhythms in nature, such as the opening and closing of flowers or our patterns of sleep and wakefulness and their association with the perpetual alteration of night and day, were recognized in antiquity although their origins were not questioned until the eighteenth century. The French Astronomer Jean-Jacques d’Ortous de Mairan conducted an investigation into whether the leaves of the Mimosa plant opened in response to light.¹ While de Mairan’s experiments were the first to question the origin of such daily rhythms, Augustin Pyramus de Candolle is credited with the first suggestion that they arose through an internal timekeeping …

A Synthetic Porcine Reproductive And Respiratory Syndrome Virus Strain Confers Unprecedented Levels Of Heterologous Protection, Hiep Vu, Fangrui Ma, William W. Laegreid, Asit K. Pattnaik, David Steffen, Alan R. Doster, Fernando Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

Current vaccines do not provide sufficient levels of protection against divergent porcine reproductive and respiratory syndrome virus (PRRSV) strains circulating in the field, mainly due to the substantial variation of the viral genome. We describe here a novel approach to generate a PRRSV vaccine candidate that could confer unprecedented levels of heterologous protection against divergent PRRSV isolates. By using a set of 59 nonredundant, full-genome sequences of type 2 PRRSVs, a consensus genome (designated PRRSV-CON) was generated by aligning these 59 PRRSV full-genome sequences, followed by selecting the most common nucleotide found at each position of the alignment. Next, the …

Efficiency Of Energy Utilization And Fecal Mineral Excretion In Holstein And Jersey Dairy Cows, Gabriel Garcia Gomez

Department of Animal Science: Dissertations, Theses, and Student Research

Eight multiparous Holstein and 8 Jersey dairy cows were used to complete energy balance trials designed to determine the effect of breed on the production and energy efficiency of milk, and energy partitioning. A repeated switchback design was used to compare the effect of cow breed. Diets consisted of 24.5% corn silage, 18.4% alfalfa hay, 6.9% grass hay, with either 22.9% rolled corn and 14.8% soybean meal (CON) or 8.95% rolled corn and 28.8% reduced fat distillers grains (RFDDGS) DM-basis. Diets were offered ad libitum for a 28 d adaptation period and 95% ad libitum for a 4 d collection …

General Anesthesia, Utilizing Etorphine Hydrochloride And Azaperone With Supplementary Isoflurane Inhalation Anesthesia, For Gastro-Esophageal Endoscopy In A Domesticated Asian Elephant (Elephas Maximus): A Case Report, Nikorn Thongtip, Aumaporn Maikaew, Wanlaya Tipkantha, Daraka Thongthainan, Nicharee Income, Benjapol Lorsunyaluck, Pornchai Sunyathitiseree, Boripat Siriaroonrat, Sumate Kamolnoranarth

The Thai Journal of Veterinary Medicine

A 30-year-old female domestic Asian elephant (Elephas maximus) was referred to Kasetsart University Veterinary Teaching Hospital for investigation of an esophageal obstruction. “Vomiting” had been noted for several days before admission. Initial sedation was achieved by azaperone (80 mg), delivered intramuscularly (IM). Once sedated supporting straps and a sling were fitted, and weight-bearing support was provided by a crane. General anesthesia was induced using 2 mg etorphine hydrochloride (0.00066 mg/kg) by intravenous (IV) injection. Using the crane, she was lowered onto a thickly bedded area on the floor and placed in right lateral recumbency. Oxygen was initially provided via the …

Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram, Nawat Sannamwong

The Thai Journal of Veterinary Medicine

No abstract provided.

Evaluation Of Bacillus Spp Candidates For Aflatoxin B1 Biodegradation In Broiler Chickens, Rosario Galarza Seeber

Graduate Theses and Dissertations

Poultry health has been traditionally maintained by hygenic measures, vaccinations, and the use of antibiotics. Modern husbandry management considers the use of probiotics as a natural way to protect birds against many everyday pathogens. Different strains of Bacillus spp. have proved to have beneficial effects in poultry production. However, the most used bacterium in commercial probiotics is Lactobacillus, a vegetative cell. In contrast, Bacillus spp are bacterial spores, highly resistant to harsh conditions, which makes them preferable, in some cases, to Lactobacillus because of shelf life and storage conditions. There is published information regarding mycotoxin detoxification by bacteria. Mycotoxins are …

Induction Of Parturition In Sows, Roy N. Kirkwood

The Thai Journal of Veterinary Medicine

When levels of neonatal mortality are considered too high or there is a need for relatively high levels of cross fostering, a protocol of induced farrowing should be implemented to facilitate farrowing supervision. Before implementing an induction protocol the normal gestation length for the farm should be established and sows not induced more than 2 days in advance. Earlier injections will result in low birthweight pigs with potentially compromised lung development. For optimal induction, inject prostaglandin or an analogue into the vulva at the label or 50% dose in the morning and again 6 to 8 hours later. Do not …

Quantitative Method For Detecting Listeria Species Using The Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Saburo Ohkawa, Yukio Morita, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

We developed a quantitative method for detecting Listeria species in food using the DOXTM system. The system could detect low concentrations (10 cfu/ml) of Listeria monocytogenes, L. innocua, and L. welshimeri, and could not detect medium concentrations (103 cfu/ml) of the 29 non-Listeria organisms examined in this study. The relationship between detection time and bacterial count of the 3 Listeria species had a good linear calibration curve. We carried out a recovery examination by inoculating 15 food samples with L. monocytogenes, and each sample was examined 5 times. L. monocytogenes was detected in 68 of the 75 samples inoculated with …

Effects Of Varying Levels Of Liquid Dl-Methionine Hydroxy Analog Free Acid In Drinking Water On Production Performance And Gastrointestinal Tract Of Broiler Chickens At 42 Days Of Age, Kanokkarn Poosuwan, Chaiyapoom Bunchasak, Jumroen Thiengtham, Kanchana Markvichitr, Somchai Chansawarng, Apassara Chutesa

The Thai Journal of Veterinary Medicine

This study was conducted to evaluate the effects of adding liquid DL-methionine hydroxyl analog free acid (LMA) to drinking water on the growth performance and gastrointestinal tract in broiler chicks during 1-42 days of age. The chicks were divided into 5 groups as follows, Group 1 (negative control); chicks received a methionine (Met) deficient diet without LMA supplementation in drinking water. Group 2, 3, 4 and 5 were diets supplemented with DL-methionine (DLM) that meet total sulfur amino acids (TSAA) requirement, then each group was graded level with LMA in drinking water at 0%, 0.025%, 0.05% and 0.1%, respectively. Average …

The Use Of Omega-3 Concentrate To Relieve Coxofemoral Osteoarthritic Pain In Dogs, Amornrate Sastravaha, Nirut Suwanna, Chayakrit Sinthusingha, Jatuporn Noosud, Em-On Olanratmanee, Atthaporn Roongsitthichai

The Thai Journal of Veterinary Medicine

The current study aimed to assess the effect of using omega-3 concentrate to relieve coxofemoral osteoarthritic pain in dogs. In total, ten dogs with coxofemoral OA were orthopedically evaluated at the coxofemoral joints in order to grade the pain severity. Pain scores were assessed from lameness at walk and trot (1-6), pain on manipulation (1-3), and range of motion (1-4). All dogs orally received omega-3 concentrate (Omacor®, Banner Pharmacaps Europe BV, the Netherlands) for successively four weeks. During the study, they were evaluated for pain scores on a weekly basis. The results revealed that mean body weight of the patients …