Veterinary Medicine Commons^™

Effects Of Wintering System On Cow And Calf Performance In A Summer- Calving Intensive Production System, Shelby E. Gardine, Jason M. Warner, Curtis J. Bittner Bittner, Robert G. Bondurant, Karla Jenkins, Richard J. Rasby, Matt K. Luebbe, Galen E. Erickson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A study evaluated the effects of two wintering systems (cornstalk grazing and drylot feeding) on cow- calf performance in a summer- calving intensively managed cowherd at two locations. Grazing cow- calf pairs on cornstalks resulted in lower ending BW of cows and reduced ADG of calves when compared to drylot cow- calf pairs at weaning. A partial budget of incorporating winter cornstalk grazing into an intensive production system suggests that cows wintered on cornstalks were $136.85 more profitable when compared to cows wintered in the drylot.

Effects Of Modified Distillers Grains And Corn Ratios As Supplements In Diets Varying In Forage Quality On Performance Of Growing Beef Steers, Viviane B. Ferrari, Robert G. Bondurant, Galen E. Erickson Erickson, Terry J. Klopfenstein, Jim C. Macdonald

Nebraska Beef Cattle Reports

Distillers grains (DG) have been intensively used for beef production, but prices relative to corn vary such that it may be beneficial to displace a portion of DG with corn in certain circumstances. Four ratios of supplemental energy (40% of diet DM) from modified distillers grains and corn (100:0, 80:20, 60:40, and 40:60) were supplemented in one of 3 forage diets (56% of diet DM) and evaluated for performance. Forages were high (brome hay + sorghum silage) or low quality. Low quality forages were corn residue baled through conventional rake and bale, or by disengaging the spreader on the combine …

Fatty Acid Composition Of Beef Fed Omnigen- Af At Receiving Or Finishing, Katherine I. Domenech, Michael D. Chao, Joe O. Buntyn Buntyn, Ty B. Schmidt, Chris Calkins

Nebraska Beef Cattle Reports

Beef fatty acid profiles and superoxide dismutase activity were determined for cattle receiving OmniGen- AF supplementation (a patented nutritional supplement) at receiving (first 28 d at the feedlot) or throughout finishing (all 210 d of finishing) vs. a control group (non- supplemented). Th e most meaningful change in fatty acid composition from inclusion of OmniGen- AF was total poly- unsaturated fatty acid (PUFA) content where beef from the finishing group had more PUFA content in relation to the receiving group and was not different from the control group. Despite this increase in PUFA, cattle supplemented through finishing tended to have …

Effect Of Backgrounding System On Steer Performance And Carcass Characteristics, Jordan L. Cox, Kristin E. Hales, Kristen M. Ulmer Ulmer, Richard J. Rasby, Steven D. Shackleford, Chad Engle, John Rieckman, Harvey C. Freetly, Mary Drewnoski

Nebraska Beef Cattle Reports

The impact of 3 backgrounding systems: grazing corn residue with distillers grains supplementation at 0.86% BW/d, grazing an oats-brassica forage, or feeding a grower ration in a drylot on finishing performance and carcass characteristics were evaluated. Backgrounding phase gains were greatest for steers fed a grower ration in the drylot (3.58 lb/d), intermediate for steers grazing oats-brassica forage and then fed the grower ration for short period (2.65 lb/d), and least for steers grazing corn residue while supplemented distillers grains and then fed the grower ration for short period (2.22 lb/d). These backgrounding treatment differences did not affect ADG during …

Shelf Life Of Ground Beef From Cattle Fed Distillers Grains Containing Different Amounts Of Oil, Faith D. Rasmussen, Chad G. Bower, Gary A. Sullivan Sullivan

Nebraska Beef Cattle Reports

Beef shoulder clods were collected from steers fed one of four finishing diets: no distillers grains and three with distillers grains containing different amounts of oil. Raw ground beef patties were evaluated for changes in objective color, discoloration, and lipid oxidation during simulated retail display (7 days). Cooked beef links in refrigerated (18 days) and frozen storage (198 days) were analyzed for lipid oxidation throughout shelf life. Fatty acid profiles were evaluated in lean, subcutaneous fat, and ground composite samples. All distillers grain diets increased C18:2 and polyunsaturated fatty acids in beef. There were no dietary differences in lipid oxidation …

Rumen Undegradable Protein And Bambermycins Supplementation Of Calves Grazing Corn Residue, Cody A. Welchons, Robert G. Bondurant, F. Henry Hilscher Hilscher, Jim C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Steer calves grazing non- irrigated corn residue were supplemented with a corn residue and by- product pellet that contained either Soypass, soybean meal, or processed soybean meal at a rate of 4 lb / d. Additionally, a supplement was fed at 1 lb / d that provided 0 or 20 mg / steer daily of bambermycins (Gainpro®). There was no interaction between bambermycins inclusion and pellet type. Likewise, there was no effect of pellet type or bambermycins on ending BW or ADG. In order to maximize gain of calves grazing corn residue it is important to provide a supplement that …

Delineating Complex Forage Mixtures Using Plant-Wax Markers, Napoleón Vargas Jurado, Hannah C. Hamilton, Ronald M. Lewis

Nebraska Beef Cattle Reports

Plant waxes provide a marker profile of individual plants that, when sufficiently distinct, can be used to estimate the diet composition of grazing cattle. They also may provide a tool for reliably predicting feed intake. The traditional method (nonnegative least squares) to use these markers to predict diet composition has limitations. A newer statistical approach (Bayesian linear unmixing) deemed more efficient was tested with simulation. Samples from 8 forage species in Nebraska were analyzed for their plant- wax marker contents. Those concentrations were used to simulate 1000 diets for 4 mixtures containing 2, 3, 5 or 8 plants. The efficiency …

Effect Of Corn Residue Grazing Or Baling On Subsequent Crop Yield And Nutrient Removal, Kristen M. Ulmer, Jordan L. Cox, Manbir K. Rakkar Rakkar, Robert G. Bondurant, Humberto Blanco-Canqui, Mary Drewnoski, Karla H. Jenkins, James C. Macdonald, Rick J. Rasby

Nebraska Beef Cattle Reports

A 2- year study evaluated the effect of corn residue baling or grazing on subsequent yields, as well as, nutrient removal by baling at five locations in Nebraska. Three treatments were applied to each field: baled, grazed, and control (no baling and no grazing). Grain and stover yields were measured by hand harvest at maturity. No differences were observed among treatments for corn yield with baled having yields of 234 bu grain / acre, grazed with 239 bu grain / acre and control with 223 bu grain / acre. Th ere was no difference in stover yield among treatments (8,326, …

Timed Insemination Vs. Modified Estrus Detection In Beef Heifers, Benjamin T. Tibbitts, Tonya L. Meyer, Dan J. Kelly Kelly, Richard N. Funston

Nebraska Beef Cattle Reports

Th e objective of this study was to compare a modified estrus detection and fixed time AI vs. no estrus detection and fixed time AI on subsequent pregnancy rates. Yearling heifers were estrus synchronized and AI at 72 ± 2 h after prostaglandin injection. In one group estrus was not detected and all heifers received gonadotropin releasing hormone at the fixed- time AI; in the other group estrus was detected at 58 ± 2 and 70 ± 2 h after prostaglandin and inseminated in the following order at 72 ± 2 h: heifers in estrus at 58 h, heifers in …

Growth And Reproductive Performance Of Yearling Beef Heifers Implanted With Revalor G In The Nebraska Sandhills, Benjamin T. Tibbitts, Hazy Rae Nielson, K. H. Ramsay Ramsay, Richard N. Funston

Nebraska Beef Cattle Reports

Th is study evaluated effects of a single stocker implant (Revalor G) on growth and reproductive performance of yearling beef heifers in the Nebraska Sandhills. Crossbred heifers, grazing native Sandhills range, were randomly assigned to either be implanted 82 ± 2 days prior to estrus synchronization, or not implanted, to determine the effects of growth implants on heifer performance. Heifers were bred via artificial insemination followed with clean- up bulls. Implanted heifers gained more and were heavier at the end of the trial, but had a reduced pregnancy rate than non- implanted heifers. Implanted heifers also had a lower pregnancy …

Impact Of Heifer Development System In Two Different Breeding Seasons, Shelby A. Springman, Hazy Rae Nielson, Jacqueline A. Musgrave Musgrave, John Nollette, Andy Applegarth, Richard N. Funston

Nebraska Beef Cattle Reports

Replacement heifers from March and May calving herds were offered ad libitum meadow hay and 4 lb/d supplement or grazed meadow and offered 1 lb/d supplement from mid- January to mid- April. Heifers fed hay gained more during the treatment; however, heifers grazing meadow experienced compensatory gain, resulting in similar body weight at pregnancy diagnosis in both calving herds. Pregnancy rates were similar between treatment groups in March and May heifers. A reduced input winter management system is a viable option to maintain pregnancy rates in early and late summer breeding seasons.

Effects Of Distillers Grains Or Fiber On Enterohemorrhagic Escherichia Coli In Steers, Liesel G. Schneider, Curtis J. Bittner, Jordan E. Burhoop Burhoop, F. Henry Hilscher, Galen E. Erickson, Terry J. Klopfenstein, Rodney A. Moxley, David R. Smith

Nebraska Beef Cattle Reports

Increased probability for fecal shedding and colonization by Escherichia coli O157:H7 has been observed in previous studies when steers are fed increased inclusions of distillers grains. A feeding study was conducted to determine if distillers grains or fiber from distillers grains in the finishing ration of feedlot steers affected fecal shedding prevalence of seven serogroups of enterohemorrhagic Escherichia coli (EHEC- 7). For EHEC O45 and EHEC O103, the greatest prevalence of shedding occurred when steers were fed increased distillers grains For all EHECs except for O111, fecal shedding prevalence was similar between the corn control and either of the corn …

Effect Of Long- Term Corn Residue Grazing On Soil Properties, Manbir K. Rakkar, Humberto Blanco, Mary E. Drewnoski Drewnoski, Jim C. Macdonald, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the effect of long- term (16 years) corn residue grazing with cattle on soil compaction, soil structure, soil organic matter, and nutrients. Three treatments: 1) fall grazing (November through January; 1.8 to 2.5 AUM/ac), 2) spring grazing (February to middle April; 2.3 to 3.1 AUM/ac), and 3) control (no grazing) under a no- till irrigated corn- soybean system in eastern Nebraska were studied. Crop yields were increased and soil bulk density and cone index (parameters of soil compaction), wet soil aggregate stability (parameter of soil structural quality), and organic matter content were not affected …

Effect Of Increasing Supplemental Rumen Undegradable Protein (Rup) On Performance Of Calves Fed A Silage Growing Diet, Colton R. Oney, F. Henry Hilscher, Robert G. Bondurant Bondurant, Andrea K. Watson, Galen E. Erickson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A growing study evaluated the effects of increasing supplemental rumen undegradable protein (RUP) on performance of calves fed an 85% corn silage diet. Five levels of supplementation were evaluated with 12 individually fed steers per level of supplement. Supplement levels consisted of 0, 3.25, 6.5, 9.75 and 13% supplemental RUP (% of diet DM) fed as a blend of 60% Soypass and 40% Empyreal. Increasing supplemental RUP in the diet increased ending body weight and average daily gain linearly, and decreased F:G linearly while DMI remained constant among treatments. As cattle grew from 600 to 730 lb, F:G was improved …

2017 Nebraska Beef Cattle Report (Complete)

Nebraska Beef Cattle Reports

Contents

Cow/Calf: Effect of Heifer Development System on Reproduction and Subsequent Gain as a Pregnant Heifer • Impact of Heifer Development System in Two Breeding Seasons • Timed Insemination vs. Modifi ed Estrus Detection in Beef Heifers • Growth and Reproductive Performance of Yearling Beef Heifers Implanted with Revalor G in the Nebraska Sandhills • Development of Terminal and Maternal Economic Selection Indices in Beefmaster Cattle • Effects of Wintering System on Cow and Calf Performance in a Summer- Calving Intensive Production System • Methods to Increase Productivity of Spring Calving Production Systems in the Nebraska Sandhills

Growing: Effect of …

In Situ Digestibility Of Residue Parts Of Corn Planted In Different Populations And Row Widths, Viviane B. Ferrari, Janessa J. Updike, Jana Harding Harding, Keith Glewen, Terry J. Klopfenstein, Jim C. Macdonald

Nebraska Beef Cattle Reports

Plant density can be changed by altering row width and/or number of plants within a row. The use of narrower rows at seeding may increase yield and reduce plant-toplant competition. Corn seeds were planted at 2 row widths (15 and 30- inch rows) and 3 plant populations (25,200, 35,000 and 54,000 seeds / acre). Samples of corn residue were collected 4 dates (October 8, October 24, November 5 and November 19) postharvest and separated into cob, stem, leaf and husk to determine changes in quality over time. Th e greatest plant population increased the NDF content and NDF digestibility of …

Microfluidics-Based Single Cell Isolation And Trapping Of Canine Cutaneous Mast Cell Tumor Cells, Dettachai Ketpun

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this research, the primary objective was to develop a microfluidic platform utilized for the size-based single cell sorting of canine cutaneous mast cell tumor (MCT) cells which were categorized into 4 groups; I, II, III and IV, upon their individual sizes ranged from 5-9, 10-14, 15-19, and equal to or over than 20 mm respectively. And the secondary aim was to establish the microfluidic-based entrapping microdevice which had a capacity to ensnare single MCT cells into its triangular microwell array at the ratio of one cell per one microwell. The study consequence consistently indicated the accomplishment of device developments. …

The Indirect Effect Of Bvdv On Immune Suppression: The Role Of Infected Macrophages In Lymphocyte Apoptosis, Karim Abdelsalam

Electronic Theses and Dissertations

BVDV is an important pathogen of cattle that affects both the dairy and beef industry causing severe economic losses. The main problem of BVDV is the immune suppression that is associated with lymphoid depletion shortly post infection. The mechanism of this immune suppression is not well understood. In this in vitro study, we sought to determine the indirect effect of BVDV infection in macrophages as a key player in the pathogenesis of BVD especially, the apoptotic effect induced on lymphocytes. We infected bovine monocyte-derived macrophages (MDM) with either high or low virulent strains of cytopathogenic or non-cytopathogenic strains of BVDV …

Intranasal Immunization Of Pigs With Porcine Reproductive And Respiratory Syndrome Virus Like Particles Plus 2’, 3’ –Cgamp Vaccigrade™ Adjuvant Exacerbates Viremia After Virus Challenge, Alexandria Marie Van Noort

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically devastating virus that causes a persistent infection and spreads quickly. The virus causes abortions in sows and respiratory diseases in young piglets. Current PRRSV vaccines appear to provide limited cross-protection. A virus-like particles (VLPs) vaccine is made up of key PRRSV structural proteins, which could be cross protective among the different strains. We tested PRRSV VLPs as a vaccine candidate with a 2’, 3’-cGAMP VacciGradeTM adjuvant against pigs infected with PRRSV after intranasal immunization to evaluate the immune response and protective efficacy by the VLPs and adjuvant. The VLPs contain …

Surveillance Of Emerging Livestock Viruses, Faten A. Okda

Electronic Theses and Dissertations

Accurate and rapid diagnostic tests are essential for the management and control of emerging infectious diseases. Therefore, the overall goals of studies presented in this dissertation were to provide improved diagnostic and surveillance capabilities for several emerging viral diseases impacting the livestock industry and to provide insight into appropriate control strategies. Recent, severe outbreaks of porcine epidemic diarrhea virus (PEDV) in Asia and North America highlighted the need for well-validated diagnostic tests for identification of PEDV infected animals and evaluation of their immune status. PEDV was first detected in the U.S. in May 2013 and another swine coronavirus, porcine deltacoronavirus …

Understanding The Effect Of Bvdv On Innate Immune Response Of Neutrophils, Neelu Singh Thakur

Electronic Theses and Dissertations

Bovine viral diarrhea virus (BVDV) is one of the highly prevalent and economically important diseases of cattle industry worldwide. The two major consequences of this disease are persistent infection and immunosuppression. Several studies have been done to determine the underline mechanisms of BVDV-induced immunosuppression targeting antigen presenting cells, adaptive immune system cells and cytokine gene expression. However, very little research has been done to determine the effect of BVDV on neutrophils. Neutrophils are one of the most abundant while blood cells (WBC) in the peripheral blood, which play a critical role in the innate as well as adaptive immune response. …

Immune Response In The Mouse Cornea Elicited By Herpes Simplex Vaccine Vc2, Logan C. Kinamore

Honors Capstones

No abstract provided.

Reagents And Tools To Identify Epizootic Hemorrhagic Disease Virus-2, Meghan Syrstad

Electronic Theses and Dissertations

The purpose of this project was to define monoclonal antibodies against viral proteins from Epizootic Hemorrhagic Disease Virus (EHDV) to be used in quality control of vaccines produced by an industrial partner, and development of tools to identify EHDV. EHDV is an Orbivirus, which also includes Bluetongue Virus and African Horse Sickness, which are all transmitted through arthropod vectors. EHDV causes significant morbidity and mortality in white-tailed deer, but has recently been found to infect cattle. EHDV recently caused significant outbreaks affecting both the farmed and wild cervid industry, however few reagents and tools exist to protect against this disease …

Senecavirus A: Epidemiology, Pathogenesis And Infection Dynamics, Lok Raj Joshi

Electronic Theses and Dissertations

Senecavirus A (SVA) is a positive sense single-stranded virus that belongs to the genus Senecavirus within the family Picornaviridae. Senecavirus has been present in US swine population since 1988. However, there have been only a few reports of SVA associated with disease in last two decades. In late 2014 early 2015, a sudden increase in the number of cases of Senecavirus A associated with vesicular disease was observed in Brazil, the US and China. Affected pigs presented vesicles on snout, coronary band, and dewclaw. Additionaly, increased neonatal mortality has been reported in the field. Many aspects of SVA epidemiology, …

Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya

Turkish Journal of Veterinary & Animal Sciences

The purpose of the study was to use decision trees to predict increased levels of somatic cells in cow's milk. The material for the study comprised data collected in 2012-2014 from five farms in Poland equipped with an automatic milking system. Data on 803 Polish Holstein-Friesian cows were collected. In order to predict somatic cell count data mining techniques were used to build a graphical model of a decision tree. This study found that the most important factors to anticipate an elevated somatic cell count in cow's milk are milk conductivity, lactation stage, and lactation (primiparous and multiparous cow groups), …

Effective Use Of Probiotic-Glyconutrient Combination As An Adjuvant To Antibiotic Therapy For Diarrhea In Rearing Dairy Calves, Gilberto Lopez Valencia, Octavio Zapata-Ramirez, Lilia Núñez-González, Victor Núñez-Benítez, Heriberto Landeros- López, Maria Lopez-Soto, Alberto Barreras Serrano, Victor Gonzalez-Vizcarra, Alfredo Estrada-Angulo, Richard Zinn, Alejandro Plascencia

Turkish Journal of Veterinary & Animal Sciences

Thirty Holstein calves presented with infectious diarrhea during the second week following birth were utilized to evaluate the effects of a standardized product containing a combination of probiotics, ß-glucans, and glyconutrients (GLY) in support of antibiotic therapy during the early rearing phase (day 7 to day 45 following birth). Treatments were 1) antibiotic therapy (trimethoprim and sulfas; ANTIB), and 2) ANTIB plus 5 g/calf per day GLY. Treatments did not affect diarrhea duration, averaging 16.7 days. However, GLY support decreased (P < 0.05) the mortality rate and increased (P < 0.05) live weight gain. Increased weight gain was attributable to enhanced (P < 0.05) consumption of calf starter. Supplemental GLY increased (P < 0.05) serum glucose and lowered (P < 0.05) serum urea nitrogen, nonesterified fatty acids, and cortisol concentration. We conclude that daily provision of 5 g of a standardized product containing probiotics, ß-glucans, and glyconutrients to diarrheic calves under antibiotic therapy will promote health and enhance early growth performance.

Detoxification Of Mitochondrial Oxidants And Apoptotic Signaling Are Facilitated By Thioredoxin-2 And Peroxiredoxin-3 During Hyperoxic Injury, Benjamin J. Forred, Darwin R. Daugaard, Brianna K. Titus, Ryan R. Wood, Miranda J. Floen, Michelle L. Booze, Peter F. Vitiello

School of Veterinary and Biomedical Sciences: Faculty Publications

Mitochondria play a fundamental role in the regulation of cell death during accumulation of oxidants. High concentrations of atmospheric oxygen (hyperoxia), used clinically to treat tissue hypoxia in premature newborns, is known to elicit oxidative stress and mitochondrial injury to pulmonary epithelial cells. A consequence of oxidative stress in mitochondria is the accumulation of peroxides which are detoxified by the dedicated mitochondrial thioredoxin system. This system is comprised of the oxidoreductase activities of peroxiredoxin-3 (Prx3), thioredoxin-2 (Trx2), and thioredoxin reductase-2 (TrxR2). The goal of this study was to understand the role of the mitochondrial thioredoxin system and mitochondrial injuries during …

Maternal Inflammation At Mid-Gestation In Pregnant Rats Impairs Fetal Muscle Growth And Development At Term, C. N. Cadaret, K. A. Beede, E. M. Merrick, T. L. Barnes, J. D. Loy, D. T. Yates

School of Veterinary and Biomedical Sciences: Faculty Publications

Intrauterine growth restriction (IUGR) is a leading cause of perinatal morbidity and mortality. Low birth weight resulting from preterm birth and/or IUGR is an underlying factor in 60–80% of perinatal death worldwide, and is particularly common in developing countries (UNICEF, 2008). Furthermore, studies have linked IUGR and the associated fetal malnutrition to increased incidence of metabolic syndrome in adult life (Barker et al., 1993; Godfrey and Barker, 2000). The “thrifty phenotype hypothesis” developed by David Barker (Hales et al., 1991) states that IUGR-associated fetal malnutrition forces the fetus to spare nutrients by altering tissue-specific metabolism in order to survive. In …

A Parapoxviral Virion Protein Targets The Retinoblastoma Protein To Inhibit Nf-Κb Signaling, Ponnuraj Nagendraprabhu, Sushil Khatiwada, Sabal Chaulagain, Gustavo A. Delhon, Daniel L. Rock

School of Veterinary and Biomedical Sciences: Faculty Publications

Poxviruses have evolved multiple strategies to subvert signaling by Nuclear Factor κB (NF-κB), a crucial regulator of host innate immune responses. Here, we describe an orf virus (ORFV) virion-associated protein, ORFV119, which inhibits NF-κB signaling very early in infection (≤30 min post infection). ORFV119 NF-κB inhibitory activity was found unimpaired upon translation inhibition, suggesting that virion ORFV119 alone is responsible for early interference in signaling. A C-terminal LxCxE motif in ORFV119 enabled the protein to interact with the retinoblastoma protein (pRb) a multifunctional protein best known for its tumor suppressor activity. Notably, experiments using a recombinant virus containing an ORFV119 …

The Effect Of Whole Body Vibration On Equine Hoof Growth, Sharlee Lowe

Undergraduate Honors Thesis Projects

Equine foot pain can be a result of many conditions and disease processes, all of which lead to a loss of function in the horse. When the horse loads the foot, pressure applied to the frog translates to the digital cushion and lateral cartilages, compressing the blood vessels, perfusing the area, and circulating blood back up the leg; this constant flow of blood stimulates growth of the hoof wall. Whole body vibration plates are thought to result in rapid muscle contractions and subsequently, increased circulation through the foot. An increase in hoof growth could prove whole body vibration beneficial as …