Veterinary Medicine Commons^™

Diagnostic Approaches And Therapeutic Evaluation In Canine Benign Prostatic Hyperplasia: An Integrated Analysis Of Prostatic Volume, Serum Canine Prostatic Specific Esterase (Cpse) Levels, And Proteomic Profiles, Panthipa Borikappakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Benign prostatic hyperplasia (BPH) is a highly prevalent condition in intact male dogs. However, its diagnosis and clinical management remain limited by the lack of clear thresholds for clinically significant prostatic enlargement, insufficient biomarkers to monitor treatment response, and an incomplete understanding of the molecular mechanisms driving disease progression. To address these gaps, this thesis integrated ultrasonographic, biochemical, and proteomic approaches across three complementary studies. First, the actual to expected normal prostatic volume ratio was evaluated to establish a clinically meaningful cutoff distinguishing subclinical from clinical BPH, providing an imaging-based indicator for early recognition of clinically relevant enlargement. Building on …

Community Ecology Of Domestic Animals And Wildlife At Solid Waste Disposal Sites Near Dong Phayayen – Khaoyai Forest Complex And Its Impacts On Zoonotic Diseases, Paisin Lekcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Solid waste disposal sites (SWS) at forest edges represent critical zoonotic transmission hotspots where wildlife, domestic animals, and humans converge. This study characterized host community structure, pathogen circulation, and network topology at SWS adjacent to the Dong Phayayen-Khao Yai Forest Complex, Thailand. Three study phases (2021-2023) comprised: (1) camera trapping at five SWS (3,907 trap-nights); (2) small mammal trapping across four habitats (1,420 individuals, 12 species), ectoparasite collection (20,560 chiggers, 14 species), and molecular pathogen detection; and (3) network analysis of connectivity among habitat, host, chigger, and pathogen. Domestic dogs dominated communities (88.3%), creating simplified assemblages. Potential transmission operated through …

Optimization Of Artificial Insemination In Swine Through Gnrh Agonist Application In Boar Semen With Special Emphasis On Epithelial Penetration And Pharmacokinetics, Preechaphon Taechamaeteekul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Induction of ovulation in pigs is a widely applied technique in commercial swine farms, using hormonal treatments to improve reproductive efficiency and productivity. The GnRH agonist buserelin acetate is widely used to induce ovulation and can be administered via intramuscular or intravaginal routes. However, the success of ovulation induction via the intravaginal route still depends on several factors, and information regarding the pharmacokinetics and tissue permeation of buserelin remains limited. This thesis aimed to investigate the pharmacokinetic profile, endometrial permeability, and reproductive effects of buserelin to develop an effective and practical ovulation control strategy for commercial swine production. This thesis …

Surveillance And Genetic Characterization Of Atypical Porcine Pestivirus (Appv) In Central Thailand, Sana Shahid

Chulalongkorn University Theses and Dissertations (Chula ETD)

Atypical Porcine Pestivirus (APPV) is an emerging swine pathogen associated with congenital tremor (CT) Type A-II, a neurological condition characterized by tremors of the head, body, and limbs, as well as poor growth in piglets. From September 2023 to August 2025, nasal and blood samples were collected from 181 pigs from four commercial pig farms in Central Thailand, and APPV was detected in 17 samples. Notably, all APPV-positive samples come from one farm. A follow-up study was conducted at the infected farm to assess APPV persistence and its association with age groups and clinical signs. In total, 275 pigs were …

Genomic Characterization Of Streptococcus Agalactiae And Streptococcus Suis Isolated From Diseased Snakeskin Gourami (Trichopodus Pectoralis), Surawit Chueahiran

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigates severe, recurrent mortality outbreaks in farmed snakeskin gourami (Trichopodus pectoralis) in central Thailand between 2021 and 2023. Characterized by exophthalmos and systemic hemorrhage, these incidents prompted an investigation to identify causative agents, assess antimicrobial resistance (AMR), and explore genomic adaptation. Bacterial isolates from moribund fish in Samut Sakhon province were identified via MALDI-TOF MS and 16S rRNA sequencing, followed by whole-genome sequencing (Illumina HiSeq) and comparative pan-genomic analysis. Two primary pathogens, Streptococcus agalactiae and Streptococcus suis, were confirmed as causative agents of acute bacterial septicemia, occurring in both single and co-infections. Phylogenetic analysis revealed that S. suis …

Expression Patterns Of Alpha-Fetoprotein And Phosphodiesterase 4d In Canine Mammary Tumors, Thi Kieu Trinh Mai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine mammary tumors (CMTs) represent one of the most common neoplasms in female dogs, with a substantial proportion exhibiting malignant behavior and aggressive progression. Despite their clinical importance and relevance to comparative oncology, the molecular mechanisms underlying CMT development remain incompletely understood. This study aimed to evaluate the expression patterns of alpha-fetoprotein (AFP) and phosphodiesterase 4D (PDE4D) in canine mammary tissues, and to investigate their correlations with Ki-67, a standard marker of cell proliferation. A total of 50 dogs were included in this study (40 diagnosed with CMTs and 10 without CMTs), yielding 90 FFPE tissue samples for protein analysis …

Leveraging Clinical Standards To Improve The Translational Relevance Of Therapies For Experimental Spinal Cord Injury, Aaron L. Silverstein

Theses and Dissertations--Neuroscience

Efforts to improve treatment of spinal cord injury (SCI) often use animals to develop effective therapeutics, intending to improve the survival and the quality of life of persons with SCI. Over half of all SCIs in the United States occur at the cervical level and often cause locomotor deficits and life-threatening breathing dysfunction. Thus, my dissertation is focused on this important patient population.

After beginning with a description of translational research and surveying SCI from a clinical standpoint, I turned to describe experimental models of cervical SCI and more detailed neuroimmunological findings relevant to my work. Then, I conducted a …

Gene Expression In Relation To Bone Fractures In Throughbred Racehorses, Anna M. Rauber-Ramos

Theses and Dissertations--Veterinary Science

Evidence that fractures in Thoroughbred racehorses are outcomes of chronic bone microdamage has led to extensive research into identifying horses at increased risk of fracture. This includes the use of biomarkers and messenger RNA (mRNA) as a measure of bone turnover. Previous research has found expression differences in horses with fractures compared to controls. Utilizing pre-race whole blood samples collected over an 18-month period from Thoroughbred racehorses in Southern California, RNA-sequencing was conducted on horses with bone fractures and race-matched controls to identify differences in gene expression. Focusing on third metacarpal (MC3) and proximal sesamoid bone (PSB) fractures, differentially expressed …

Engineering Novel Biomimetic Models Of Equine Host-Microbe Interactions, Lynn M. Leedhanachoke

Theses and Dissertations--Veterinary Science

One major limitation to the study of equine disease is the lack of in vitro models that accurately reflect the dynamic physiology of the horse. Thus, there is a pressing need to develop in vitro systems that recapitulate diverse equine tissue and organ microenvironments. To overcome this deficiency, we developed multiple biomimetic equine organoid systems that can be employed for a variety of investigations aimed at resolving outstanding questions in infectious disease pathogenesis, therapeutic development and drug metabolism, host defense mechanisms, immune-modulated disease, and equine developmental biology. Our innovative platform incorporates novel equine intestinal enteroid and airway organoid models derived …

Impacts Of Early/Mid-Gestation Shearing On Umbilical Blood Flow And Lamb Birth Weight, Mara Johnson

Electronic Theses and Dissertations

Studies outside the USA have reported that shearing pregnant ewes during mid to late gestation increases lamb birth weight. Our hypothesis was that shearing pregnant ewes on day 50 of gestation would increase umbilical blood flow (UBF), fetal size, lambing and placental weights. Twenty-eight Polypay ewes were randomly assigned to two treatments: shorn (S) and sham-shorn (Control, CON) and divided in singleton (S: n=4; CON: n=4), twins (S: n=5; CON: n=5) and triplets (S: n=5; CON: n=5). Umbilical BF and fetal and placental measurements were obtained via ultrasonography every 10 days from day 50 to 110 of gestation. Lamb birth …

Effect Of Forage Allocation On Steer Performance When Grazing Cereal Rye, Shelby L. Davies-Jenkins, Abigail M. Sartin, Daren D. Redfearn, Mary E. Drewnoski

Nebraska Beef Cattle Reports

Summary with Implications

Cereal rye is the most common cover crop planted today. With its good winter tolerance and rapid spring growth during a gap in perennial pasture productivity, it is an attractive forage source in early spring. Unfortunately, optimum stocking rates and how they are related to forage biomass availability are unknown. The study objective was to understand the effect of forage allowance on steer performance when rotationally grazing fall-planted cereal rye in spring. Stocking rate was negatively correlated with pounds of forage offered. Surprisingly, amount of forage offered related to average daily gain. Growing steers grazing cereal rye …

Strategies Affecting Performance And Profitability Of May-Born Steers, Dempster M. Christenson, Jacki A. Musgrave, Richard N. Funston

Nebraska Beef Cattle Reports

Summary with Implications

This study compared the performance and economic outcomes of differing backgrounding and feedlot systems in May-born yearling steers. We hypothesized reduced supplementation with adequate forage availability while backgrounding weaned steers will yield a similar carcass with greater profitability. It was hypothesized yearling steers backgrounded on pasture before finishing in feedlot would have a more valuable carcass that is more profitable than steers that enter the feedlot immediately. Weaned steer calves were backgrounded with ad libitum hay and 4 lb/d supplement in drylot or on pasture with 1 lb/d supplement. As yearlings, half of each backgrounding group immediately …

Impact Of Feeding Distillers Grains Compared To New Fractionated Distillers Grains (Solbran) On Feedlot Cattle Performance And Enteric Methane, Debora G. Sousa, Rebecca L. Mcdermott, Mitchell M. Norman, James C. Macdonald, Rick Stock, Ryan A. Mass, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

This study evaluated feeding wet or dry traditional distillers grains with wet and dry fractionated distillers (Solbran) fed at 40% of diet DM compared to a corn control diet on enteric methane emissions and performance. Compared to the control, there was no difference in carbon dioxide production, but there was an increase in methane production in the steers fed fractionated distillers compared to conventional distillers. Feeding steers traditional or fractionated distillers grains increased consumption, increased gain, but improved conversions only for steers fed WDGS, while steers fed the other distillers grains had worse or the same feed …

Form And Function In Mobulids: A Comparative Analysis Of Filter Morphology With Bioinspiration Applications, J. B. Teeple, S. R. Kahane-Rapport, K. E. Cohen, L. Hamann, J. A. Strother, E. W. M. Paig-Tran

Biological Sciences Faculty Publications

Mobulas (manta and devil rays) are large-scale ram filter feeders that separate planktonic food particles from large volumes of water with minimal clogging. This contrasts with most human-made filters that can suffer from problematic clogging requiring additional mechanisms for clearing blocked surfaces and maintaining performance. Prior studies have shown that mobulas employ a unique mechanism referred to as ricochet separation to filter feed, whereby captive vortices in filter pores cause particles to bounce off the filter surfaces and away from the filter pores. This mechanism enables the filtration of particles smaller than the pore size and reduced clogging. However, few …

Assessing Reproductive Health In Zoo-Managed African Elephant Bulls (Loxodonta Africana), Kaitlyn Marie Campbell

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Populations of zoo-managed African elephant (Loxodonta Africana) exhibit substantial individual- and population-level variation in reproductive success, yet research has largely focused on females, leaving male fertility poorly understood. Subfertility in bulls, characterized by low libido and inconsistent semen quality, poses significant challenges to genetic management and long-term population sustainability. This study a) systematically evaluates semen quality, b) identifies physiological, behavioral, social, and environmental determinants of fertility, and c) advances ex situ conservation through the expansion of the African elephant sperm bank. Between January 2023 and July 2025, 209 semen collection events were conducted from 13 sexually mature bulls …

A Two-Hit Model Of Executive Dysfunction: Simulated Galactic Cosmic Radiation Primes Latent Deficits Revealed By Sleep Fragmentation, Richard A. Britten, Ella N. Tamgue, Paola Arriaga Alvarado, Arriyam S. Fesshaye, Larry D. Sanford

Department Radiation Oncology & Biophysics Faculty Publications

Future Artemis-class missions to Mars will expose astronauts to prolonged space radiation (SR), sleep disruption, and operational demands requiring greater autonomy, placing decision making and executive function at heightened risk. Both SR and sleep fragmentation (SF) independently impair cognition, yet their combined effects remain poorly understood. Using the Associative Recognition Memory and Interference (ARMIT) task, we assessed cognitive performance in male rats exposed to 10 cGy of Galactic Cosmic Ray simulation (GCRsim), SF, or both. Under well-rested conditions, GCRsim-exposed rats exhibited overt deficits in the C.1.2 stage, performing at chance when reinforcement contingencies shifted, consistent with impaired cognitive flexibility. In …

Hair Growth Rate Estimation In North American Ursids, Jennifer H. Stern, Karyn D. Rode, Craig A. Stricker, Sheriden Ploof, Cindy L. J. Roberts, Celess Edinger, Mary Humbyrd, Nate Wagner, Megan A. Owen, John P. Whiteman, Thea Bechshoft, Brent White, Kristin L. Laidre

Biological Sciences Faculty Publications

The feeding ecology of wildlife populations has important implications for individual health, population productivity and distribution patterns. For ursids (bears), food resources and feeding behaviour primarily affect population dynamics via effects on cub production and survival. Much of what is known about the feeding ecology of bears is based on analyses of tissues collected from capture-based research efforts, harvested animals or non-invasive approaches. However, inference about diet from hair has been limited by a lack of quantitative data on the timing of the moult and hair growth rates. We conducted a study to develop and test two methods of quantifying …

Trace Level Benzoylecgonine Detections In Equine Plasma/Serum – A Proposed Interim Plasma Level Regulatory “Cut-Off“, Andreas F. Lehner, Kimberly Brewer, Levent Dirikolu, Abelardo Morales Briceñod, Robert Holland, George Maylin, Clara Fenger, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Cocaine is a widely used human recreational substance routinely transferring at pharmacologically irrelevant trace level amounts to non-user humans and horses. Horses and humans rapidly demethylate cocaine to benzoylecgonine which is excreted in urine at concentrations approaching two thousand times higher than the corresponding plasma cocaine concentrations. In human workplace drug testing, these usually pharmacologically irrelevant trace level urinary detections of benzoylecgonine are handled by application of a 100 ng/ml urinary “cut-off“, as presented by the Substance Abuse and Mental Health Services Administration (SAMHSA). In horse racing several American states have long used published urinary “cut-offs“ for benzoylecgonine of from …

Effects Of Ionophore And Yeast Addition To The Diet On Starch Digestion And Rumen Andblood Parameters In Sheep With Subacute Rumen Acidosis, Şevket Evci̇, Mehmet Aki̇f Karsli, Mehmet Demi̇rci̇, Gökhan Şen

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the prevention of acidosis in ruminants, which causes yield and financial losses, and toevaluate the effects of feed additives on starch digestion. Four male Bafra yearling lambs with cannulated rumens were used in a 60-daystudy. Each lamb underwent 10 days of adaptation to a ration and 5 days of sampling for four different rations including a control ration(CR), monensin ration (MR), Saccharomyces cerevisiae ration (SR), and a ration with the combination of monensin and Saccharomycescerevisiae (MSR). The experiment followed a 4 × 4 Latin-square design. Dry matter intake and digestion rates were recorded as 2404 …

Effects Of A Diet Supplemented With A Blend Of Protected Organic Acids And Essential Oilson Growth Performance And Cecal Microbiota Composition In Broilers, Mehmet Akan, Kadi̇r Serdar Di̇ker, Serdar Özlü, Murat Çelebi̇, Seyyi̇de Sariçam İnce, Barişhan Doğan, Okan Eli̇bol

Turkish Journal of Veterinary & Animal Sciences

Organic acids (OAs) and essential oils (EOs) have several positive effects on the gut microbiota of broilers. In this study, weaimed to determine the efficacy of a blend of protected OAs and EOs [P(OA+EO)] on the growth performance and cecal microbiota ofbroiler chickens. A total of 160 Ross 308 broiler chickens, including 80 male and 80 female birds, all 1 day old, were randomly distributedbetween the control group (basal diet) and the treatment group [basal diet + 300 g/t P(OA+EO)] according to an 8-pen arrangementwith 4 replicates of 20 birds. Body weight (BW), body weight gain (BWG), feed consumption (FC), …

Characterization Of The Biofilm-Forming Ability And Certain Virulence Characters Ofaeromonas Spp. Isolates From Rainbow Trout, Türker Çelebi̇, Ertan Emek Onuk, Esra Demi̇rbaş, Gökçen Di̇nç

Turkish Journal of Veterinary & Animal Sciences

Aeromonas spp., commonly found in the aquatic environment, cause infections in both fish and humans. Their pathogenicitydepends on various virulence factors and ability to form biofilm. The current study aimed to determine the biofilm-forming capabilitiesand certain virulence characteristics of 25 Aeromonas spp. isolates obtained from rainbow trout in Türkiye. The biofilm-forming abilitiesof isolates were investigated comparatively using Congo red agar (CRA), the tube method (TM), and tissue culture plates (TCPs).Potential virulence factors, including hemolytic activity, gelatinase production, serine protease activity, DNase activity, and motility,were evaluated using phenotypic tests. Additionally, the distribution of four known virulence genes, namely aerA, exu, fla, …

Comparison Of Three Chimeric Zika Vaccine Prototypes Developed On The Genetic Background Of The Clinically Proven Live-Attenuated Japanese Encephalitis Vaccine Sa14-14-2, Byung-Hak Song, Jordan C. Frank, Sang-Im Yun, Justin G. Julander, Jeffrey B. Mason, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Xin Dai, Young-Min Lee

Animal, Dairy, and Veterinary Science Student Research

Zika virus (ZIKV) is a medically important mosquito-borne orthoflavivirus, but no vaccines are currently available to prevent ZIKV-associated disease. In this study, we compared three recombinant chimeric viruses developed as candidate vaccine prototypes (rJEV/ZIKV^MR-766, rJEV/ZIKV^P6-740, and rJEV/ZIKV^PRVABC-59), in which the two neutralizing antibody-inducing prM and E genes from each of three genetically distinct ZIKV strains were used to replace the corresponding genes of the clinically proven live-attenuated Japanese encephalitis virus vaccine SA₁₄-14-2 (rJEV). In WHO-certified Vero cells (a cell line suitable for vaccine production), rJEV/ZIKV^P6-740 exhibited the slowest viral growth, formed …

Retrospective Analysis Of Senecavirus A Emergence In Diagnostic Samples From 2010-2021 In Thailand, Parin Watcharavongtip, Patumporn Jermsutjarit, Angkana Tantituvanont, Dachrit Nilubol

The Thai Journal of Veterinary Medicine

This study investigates Senecavirus A (SVA) in Thai swine using 932 clinical samples, comprising 185 serum samples and 747 vesicular fluid and lesion tissue samples. These samples were collected from suspected cases between 2010 to 2021 at pig farms in the northern, western, and central regions of Thailand by the Swine Viral Evolution and Vaccine Development Research Unit (SVEVR) at Chulalongkorn University, Bangkok, Thailand. SVA was first detected in Thailand in 2016 and has been widespread since then, with PCR-based molecular detection showing an average prevalence of 16.3% for SVA, 30.7% for foot and mouth disease virus (FMDV), and 7.5% …

Diagnosis And Treatment Of Crop Inflammation Of Rough-Legged Buzzard (Buteo Lagopus) In Daqing Wildlife Nature Reserve, Heilongjiang Province, China, Wang Zheng, Ding Ruxin, Ge Yansong, Xu Enshuang, Zheng Jiasan

The Thai Journal of Veterinary Medicine

This article describes a Rough-legged Buzzard (Buteo lagopus) from the Wildlife Nature Reserve in Daqing, China. According to the staff of Daqing Wildlife Nature Reserve, the Rough-legged Buzzard was found to have impaired flight ability, depression, and loss of aggression, but its feathers were full and there were no obvious external injuries, so it was sent to the Wildlife Rehabilitation Center of the Teaching Animal Hospital of Heilongjiang Bayi Agricultural University for treatment. After the vet conducted X-ray and blood tests, he diagnosed the bird with a foreign body in the crop and crop inflammation. He finally treated …

Hematology, Blood Biochemistry And Cytochemical Characteristics Of Blood Cells Of Captive Aldabra Giant Tortoise (Aldabrachelys Gigantea) In Thailand, Thanida Haetrakul, Nitiwadee Keschumras, Sirawich Srisiri, Narinthip Chaiyanont, Nisama Wongnitipat, Phatcharaporn Phuasombatcharoen, Praewa Sirichitr, Nantarika Chansue

The Thai Journal of Veterinary Medicine

Aldabra giant tortoises (Aldabrachelys gigantea), well-known pet tortoises worldwide, were classified as Appendix II by The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). This study aims to investigate the hematological, blood biochemical, and cytochemical characteristics of blood cells in healthy Aldabra giant tortoises in captivity. Blood samples were collected from the dorsal tail vein of 12 tortoises (6 males and 6 females) during May and October 2022. The hematological values showed no statistically significant differences between male and female tortoises. However, in female tortoises, calcium and phosphorus levels were significantly higher than …

Administering Tolfenamic Acid To Gilts And Sows Before Artificial Insemination Did Not Improve Litter Size But Tended To Reduce Post-Service Serum Prostaglandin F2Α Levels, Pachara Pearodwong, Nithitad Jiebna, Padet Tummaruk

The Thai Journal of Veterinary Medicine

This study examined the impact of intramuscular administration of tolfenamic acid 10 min before artificial insemination (AI) on serum PGF2α levels and reproductive performance in gilts and sows. In Experiment I, 20 gilts were divided into two groups: control (n = 10) and treatment (n = 10). Serum samples were collected 30 min before and after insemination to measure PGF2α levels. In Experiment II, 175 gilts and sows were divided into control (25 gilts and 56 sows) and treatment (16 gilts and 78 sows) groups. In both experiments, the treatment group received an intramuscular injection of 2 mg/kg tolfenamic acid …

Distinct Effects Of Extra- And Intracellular Trehalose Supplementation In Freezing Medium On The Post-Thaw Developmental Capacity Of Feline Oocytes, Saengtawan Arayatham, Wirakan Kallayanathum, Panuwat Padungros, Theerawat Tharasanit

The Thai Journal of Veterinary Medicine

Oocyte cryopreservation is a crucial technique for preserving the genetic material of female cats, yet maintaining post-thaw oocyte developmental competence remains a significant challenge. This study investigated the impact of intra- and extracellular trehalose on the developmental competence of feline oocytes following cryopreservation. In Experiment 1, immature feline oocytes were matured in vitro (IVM) with varying concentrations (0, 1.5, and 3 mM) of intracellular trehalose hexaacetate (Tre-(OAc)6). The matured oocytes were then cryopreserved and thawed prior to in vitro fertilization and embryo culture. Experiment 2 examined the effects of extracellular trehalose supplementation at concentrations of 0, 0.125, 0.25, and 0.5 …

Medication-Induced Esophageal Injury In Cats And Dogs: A Comprehensive Review, Naowarat Suthamnatpong, Aranya Ponpornpisit

The Thai Journal of Veterinary Medicine

Medication-induced esophageal injury (MIEI) is a significant concern in veterinary medicine, affecting the health and well-being of animals undergoing various pharmaceutical treatments. If not addressed promptly, MIEI can lead to severe complications such as esophagitis, esophageal stricture, perforation, and even mortality. This review aims to provide a comprehensive examination of MIEI in veterinary medicine, covering essential aspects such as the basic structure of the esophagus, the epidemiology and etiology of MIEI, and the factors influencing susceptibility. The mechanisms of injury are discussed in detail, focusing on direct mechanical injuries, chemical injuries from medication components, and systemic effects. The review also …

Interaction Of Cumulus-Oocyte Complex (Coc) Number, Oocyte Quality, And Blastocyst Numbers By Repeated Ovum Pick-Up (Opu) In In Vitro Embryo Production, Sakine Ülküm Çizmeci, Dursun Ali Dinc, Mustafa Numan Bucak, Ömer Faruk Yesilkaya, Muhammed Furkan Ciftci, Mehmet Buğra Kivrak, Ayşe Sari, Hasan Ali Cay, Maide Golbasi, Mustafa Bodu

The Thai Journal of Veterinary Medicine

Ovum pick-up (OPU) is a repeatable technique that is used for the retrieval of large numbers of immature oocytes from the antral follicles of live animals. The aim of the present study was to determine the effect of repeated OPU application on cumulus-oocyte complex (COC) number, oocyte quality, and blastocyst rates. The animal material for the study consisted of 10 Holstein heifers. Heifers selected as donors were administered OPU a total of 9 times on random days of the cycle at 1-week intervals. All antral follicles with a diameter of 2-8 mm were aspirated during the OPU procedure. In vitro …

Clinical Consideration In Total Intravenous Anesthesia Using Xylazine And Propofol In Calves, Keita Tokimitsu, Momoko Yachida, Wataru Miki, Tadashi Kuramoto, Nanako Mochizuki, Sakura Yoshida, Tadahiro Goto, Yasuhiro Morita

The Thai Journal of Veterinary Medicine

Surgery must often be performed in fields where only simple equipment and facilities are available for cattle practice, and anesthesia with minimal procedures should be improved for sufficient surgery in the field and on farms. The study aimed to investigate total intravenous anesthesia (TIVA) using xylazine and propofol to improve the anesthesia protocol for field surgeries in calves on farms. Fourteen calves undergoing laparotomy (every seven cases of herniorrhaphy for umbilical hernia repair and cryptorchidectomy) were assigned to this experiment and randomly divided into two groups using TIVA: continuous intravenous infusion with only xylazine (XY, 0.24-0.26 mg/kg/h), and xylazine and …