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Articles 2281 - 2310 of 9777

Full-Text Articles in Veterinary Medicine

Examining Traumatic Bone Fracture Healing Versus Surgical Osteotomies In Canines And Resulting Rates Of Infection, Grace Gsellman Jan 2020

Examining Traumatic Bone Fracture Healing Versus Surgical Osteotomies In Canines And Resulting Rates Of Infection, Grace Gsellman

Williams Honors College, Honors Research Projects

The development of osteomyelitis after surgical osteotomies and fracture repair in canines can propose serious health risks, ultimately leading to additional intervention from veterinarians that can become costly for pet owners and effect prognosis for the patient. Preventive measures are currently implemented during surgery and postoperatively. However, infection rates still range from 3.0% to 7.9% (Clark et al., 2018). By examining traumatic bone fracture healing versus surgical osteotomies in canines through the collection of both clinical and radiographic medical records, our primary objective was to further understand the physiological mechanism by which osteomyelitis develops. Limitations related to the collection of …


Identification Of Immunogenic Epitopes That Permit The Detection Of Antigen-Specific T Cell Responses In Multiple Serotypes Of Group B Coxsackievirus Infections, Ninaad Lasrado, Arunakumar Gangaplara, Rajkumar Arumugam, Chandirasegara Massilamany, Sayli Pokal, Yuzhen Zhou, Shi-Hua Xiang, David Steffen, Jay Reddy Jan 2020

Identification Of Immunogenic Epitopes That Permit The Detection Of Antigen-Specific T Cell Responses In Multiple Serotypes Of Group B Coxsackievirus Infections, Ninaad Lasrado, Arunakumar Gangaplara, Rajkumar Arumugam, Chandirasegara Massilamany, Sayli Pokal, Yuzhen Zhou, Shi-Hua Xiang, David Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Coxsackievirus group B (CVB) contains six serotypes that can affect various organs. Some of these organ-specific diseases such as myocarditis and pancreatitis can be caused by more than one serotype. Thus, development of immunological tools common to multiple serotypes is desired. This is especially critical for analyzing antigen-specific T cell responses at a single cell level. To this end, we made efforts to identify the immunogenic epitopes of CVB3 leading us to localize three T cell epitopes within the viral protein 1 (VP1) namely, VP1 681–700, VP1 721–740 and VP1 771–790. First, we confirmed their immunogenicity in the immunization settings. …


Methods Of Treating Or Preventing Zika Virus Infection, Shi-Hua Xiang, Nicholas Palermo, Asit K. Pattnaik, Aryamay Pattnaik Jan 2020

Methods Of Treating Or Preventing Zika Virus Infection, Shi-Hua Xiang, Nicholas Palermo, Asit K. Pattnaik, Aryamay Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

This document relates to methods and materials for treating a mammal having a Zika virus (ZIKV) infection. For example, a composition including one or more non-nucleoside RNA polymerase inhibitors can be administered to a mammal having, or at risk of developing, a ZIKV infection to treat the mammal.


Evaluation Of A Baculovirus-Expressed Vp2 Subunit Vaccine For The Protection Of White-Tailed Deer (Odocoileus Virginianus) From Epizootic Hemorrhagic Disease, Sun Young Sunwoo, Leela E. Noronha, Igor Morozov, Jessie D. Trujilo, In Joong Kim, Erin E. Schirtzinger, Bonto Faburary, Barbara S. Drolet, Kinga Urbaniak, D. Scott Mcvey, David A. Meekins, Mitchell V. Palmer, Velmurugan Balaraman, William C. Wilson, Juergen A. Richt Jan 2020

Evaluation Of A Baculovirus-Expressed Vp2 Subunit Vaccine For The Protection Of White-Tailed Deer (Odocoileus Virginianus) From Epizootic Hemorrhagic Disease, Sun Young Sunwoo, Leela E. Noronha, Igor Morozov, Jessie D. Trujilo, In Joong Kim, Erin E. Schirtzinger, Bonto Faburary, Barbara S. Drolet, Kinga Urbaniak, D. Scott Mcvey, David A. Meekins, Mitchell V. Palmer, Velmurugan Balaraman, William C. Wilson, Juergen A. Richt

School of Veterinary and Biomedical Sciences: Faculty Publications

Epizootic hemorrhagic disease virus (EHDV) is an arthropod-transmitted RNA virus and the causative agent of epizootic hemorrhagic disease (EHD) in wild and domestic ruminants. In North America, white-tailed deer (WTD) experience the highest EHD-related morbidity and mortality, although clinical disease is reported in cattle during severe epizootics. No commercially licensed EHDV vaccine is available in North America. The objective of this study was to develop and evaluate a subunit vaccine candidate to control EHD in WTD. Recombinant VP2 (rVP2) outer capsid proteins of EHDV serotypes 2 (EHDV-2) and 6 (EHDV-6) were produced in a baculovirus-expression system. Mice and cattle vaccinated …


Amphiphilic Cyclobutenes And Cylobutanes, Wantanee Sittiwong, Patrick H. Dussault, Raul G. Barletta, Robert Powers Jan 2020

Amphiphilic Cyclobutenes And Cylobutanes, Wantanee Sittiwong, Patrick H. Dussault, Raul G. Barletta, Robert Powers

School of Veterinary and Biomedical Sciences: Faculty Publications

This disclosure relates to amphiphilic compounds containing a cyclobutene or cyclobutane moiety. In some embodiments, the compounds are useful for treating infection by Mycobacterium such as Mycobacterium tuberculosis. Cyclobutene containing compounds are also useful as monomers in the preparation of amphiphilic polymers.


Mechanisms Of Sex Hormones In Autoimmunity: Focus On Eae, Ninaad Lasrado, Ting Jia, Chandirasegaran Massilamany, Rodrigo Franco, Zsolt Illes, Jay Reddy Jan 2020

Mechanisms Of Sex Hormones In Autoimmunity: Focus On Eae, Ninaad Lasrado, Ting Jia, Chandirasegaran Massilamany, Rodrigo Franco, Zsolt Illes, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Sex-related differences in the occurrence of autoimmune diseases is well documented, with females showing a greater propensity to develop these diseases than their male counterparts. Sex hormones, namely dihydrotestosterone and estrogens, have been shown to ameliorate the severity of inflammatory diseases. Immunologically, the beneficial effects of sex hormones have been ascribed to the suppression of effector lymphocyte responses accompanied by immune deviation from pro-inflammatory to anti-inflammatory cytokine production. In this review, we present our view of the mechanisms of sex hormones that contribute to their ability to suppress autoimmune responses with an emphasis on the pathogenesis of experimental autoimmune encephalomyelitis.


Porcine Reproductive And Respiratory Syndrome Virus Reverse Genetics And The Major Applications, Jayeshbhai Chaudhari, Hiep Vu Jan 2020

Porcine Reproductive And Respiratory Syndrome Virus Reverse Genetics And The Major Applications, Jayeshbhai Chaudhari, Hiep Vu

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive sense, single-stranded RNA virus that is known to infect only pigs. The virus emerged in the late 1980s and became endemic in most swine producing countries, causing substantial economic losses to the swine industry. The first reverse genetics system for PRRSV was reported in 1998. Since then, several infectious cDNA clones for PRRSV have been constructed. The availability of these infectious cDNA clones has facilitated the genetic modifications of the viral genome at precise locations. Common approaches to manipulate the viral genome include site-directed mutagenesis, deletion of viral genes or …


Viral Myocarditis Involves The Generation Of Autoreactive T Cells With Multiple Antigen Specificities That Localize In Lymphoid And Non-Lymphoid Organs In The Mouse Model Of Cvb3 Infection, Rakesh H. Basavalingappa, Rajkumar Arumugam, Ninaad Lasrado, Bharathi Yalaka, Chandirasegara Massilamany, Arunakumar Gangaplara, Jean-Jack Riethoven, Shi-Hua Xiang, David Steffen, Jay Reddy Jan 2020

Viral Myocarditis Involves The Generation Of Autoreactive T Cells With Multiple Antigen Specificities That Localize In Lymphoid And Non-Lymphoid Organs In The Mouse Model Of Cvb3 Infection, Rakesh H. Basavalingappa, Rajkumar Arumugam, Ninaad Lasrado, Bharathi Yalaka, Chandirasegara Massilamany, Arunakumar Gangaplara, Jean-Jack Riethoven, Shi-Hua Xiang, David Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Autoreactive T cells may contribute to post-viral myocarditis induced with Coxsackievirus B3 (CVB3), but the underlying mechanisms of their generation are unclear. Here, we have comprehensively analyzed the generation of antigen-specific, autoreactive T cells in the mouse model of CVB3 infection for antigens implicated in patients with myocarditis/dilated cardiomyopathy. First, comparative analysis of CVB3 proteome with five autoantigens led us to identify three mimicry epitopes, one each from adenine nucleotide translocator 1 (ANT), sarcoplasmic/ endoplasmic reticulum Ca2+ ATPase 2a (SERCA2a) and cardiac troponin I. None of these induced cross-reactive T cell responses. Next, we generated major histocompatibility complex (MHC) class …


Multi-Isotopic (Δ2h, Δ13c, Δ15n) Tracing Of Molt Origin For European Starlings Associated With U.S. Dairies And Feedlots, Scott Werner, J. W. Fischer, Keith A. Hobson Jan 2020

Multi-Isotopic (Δ2h, Δ13c, Δ15n) Tracing Of Molt Origin For European Starlings Associated With U.S. Dairies And Feedlots, Scott Werner, J. W. Fischer, Keith A. Hobson

United States Department of Agriculture Wildlife Services: Staff Publications

Introduced bird species can become invasive in agroecosystems and their management is inhibited if their origin and movements are not well understood. Stable isotope measurements of feathers can be used to infer molt origins and interstate movements in North America. We analyzed stable-hydrogen (δ2H), carbon (δ13C) and nitrogen (δ 15N) isotope ratios in feathers to better understand the molt origin of European starlings (Sturnus vulgaris) collected at dairies and feedlots throughout the United States. Primary feathers were used from 596 adult and 90 juvenile starlings collected during winter at dairies …


Tissue Tropisms Of Avian Influenza A Viruses Affect Their Spillovers From Wild Birds To Pigs, Xiaojian Zhang, Fred L. Cunningham, Lei Li, Katie Hanson-Dorr, Liyuan Liu, Kaitlyn Waters, Minhui Guan, Alicia K. Olivier, Brandon S. Schmit, Jacqueline M. Nolting, Andrew S. Bowman, Mia Kim Torchetti, Thomas J. Deliberto, Xiu-Feng Wan Jan 2020

Tissue Tropisms Of Avian Influenza A Viruses Affect Their Spillovers From Wild Birds To Pigs, Xiaojian Zhang, Fred L. Cunningham, Lei Li, Katie Hanson-Dorr, Liyuan Liu, Kaitlyn Waters, Minhui Guan, Alicia K. Olivier, Brandon S. Schmit, Jacqueline M. Nolting, Andrew S. Bowman, Mia Kim Torchetti, Thomas J. Deliberto, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Wild aquatic birds maintain a large, genetically diverse pool of influenza A viruses (IAVs), which can be transmitted to lower mammals and, ultimately, humans. Through phenotypic analyses of viral replication efficiency, only a small set of avian IAVs were found to replicate well in epithelial cells of the swine upper respiratory tract, and these viruses were shown to infect and cause virus shedding in pigs. Such a phenotypic trait of the viral replication efficiency appears to emerge randomly and is distributed among IAVs across multiple avian species and geographic and temporal orders. It is not determined by receptor binding preference …


Repellent Surface Applications For Pest Birds, Shelagh T. Deliberto, James C. Carlson, Hailey E. Mclean, Caroline S. Olson, Scott J. Werner Jan 2020

Repellent Surface Applications For Pest Birds, Shelagh T. Deliberto, James C. Carlson, Hailey E. Mclean, Caroline S. Olson, Scott J. Werner

United States Department of Agriculture Wildlife Services: Staff Publications

Common pest birds in the United States include the non-native European starling (Sturnus vulgaris), house sparrow (Passer domesticus), and the pigeon (Columba livia domestica), as well as native birds including Canada geese (Branta canadensis) and gull species (Laridae). Large concentrations of pest birds can create human health hazards and monetary losses due to consumption of crops, depredation, and fecal contamination and accumulation. Fecal contamination hazards include the potential spread of zoonotic diseases including antimicrobial-resistant zoonoses and human injury due to the accumulation of fecal material on walking surfaces. Additionally, fecal accumulation causes …


Serum Chemistry Values In Wild Black Vultures In Mississippi, Usa, Fred L. Cunningham, Sherman W. Jack, Amanda R. Deese, Eric R. Wengert, Kyle Van Why, Carla L. Huston, Scott Lemmons, Richard B. Minnis Jan 2020

Serum Chemistry Values In Wild Black Vultures In Mississippi, Usa, Fred L. Cunningham, Sherman W. Jack, Amanda R. Deese, Eric R. Wengert, Kyle Van Why, Carla L. Huston, Scott Lemmons, Richard B. Minnis

Human–Wildlife Interactions

Vultures (Cathartidae and Accipitridae) play an important role in ecosystem balance by rapidly disposing animal carcasses and thus preventing the potential spread of pathogens. Blood chemistry values provide a means of assessing the health of wildlife and wild animal populations; however, there are significant differences in chemistries among species and when comparing captive and free-living New and Old World vultures. In 2007, we collected blood serum from 30 female and 14 male wild, healthy black vultures (Coragyps atratus) live-trapped by the U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services from a power substation in …


Clinical Diagnosis, Ultrastructural Study And Antigenic Characterization Of Platynosomum Fastosum In Cats, Babi Kyi Soe Jan 2020

Clinical Diagnosis, Ultrastructural Study And Antigenic Characterization Of Platynosomum Fastosum In Cats, Babi Kyi Soe

Chulalongkorn University Theses and Dissertations (Chula ETD)

Platynosomum fastosum is a cat liver fluke causing hepatobiliary diseases in cats. To evaluate the criterior to support the suspicion index for early disease detection during subclinical infection, the biochemical parameters composed of liver, pancreatic enzymes, tumor markers were evaluated in 14 P. fastosum naturally-infected cats. As a result, alanine transaminase (ALT) and gamma glutamyl transferase (GGT) were found elevated in 57.8% (8/14) of infected cats. For the diagnostic imaging, bile sediments were present in 85.7% (12/14) of infected cats in their gallbladders. Since the number of bile-derived P. fastosum eggs were significantly higher than that from fecal samples (p=0.001), …


Fabrication Of Three-Dimensional (3-D) Corneal Transplantable Model From Silk Fibroin/Gelatin-Based Biomaterial, Chutirat Torsahakul Jan 2020

Fabrication Of Three-Dimensional (3-D) Corneal Transplantable Model From Silk Fibroin/Gelatin-Based Biomaterial, Chutirat Torsahakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine corneal blindness is a common cause of vision loss and visual impairment worldwide. While the demand of canine corneal graft is continuously elevated, the clinical useable grafts are limited to acellular biomaterials and allograft. This study aimed to generate the tissue-engineered canine cornea in the part of corneal epithelium and underlying stroma based on cLESCs (canine limbal epithelial stem cells) seeded silk fibroin and gelatin (SF/G) film and cCSSCs (canine corneal stromal stem cells) seeded SF/G scaffold respectively. Both cell types were successfully isolated by collagenase I. SF/G films and scaffolds served as the prospective substrates for cLESCs and …


Comparison Of Canine Urinary Bacterial Population In Calcium Oxalate Urolithiasis And Healthy Conditions, Nichamon Rakprakobkij Jan 2020

Comparison Of Canine Urinary Bacterial Population In Calcium Oxalate Urolithiasis And Healthy Conditions, Nichamon Rakprakobkij

Chulalongkorn University Theses and Dissertations (Chula ETD)

Urolithiasis is one of the most common lower urinary tract diseases in dogs. Struvite is the most canine uroliths reported, and the second reported is calcium oxalate (CaOx). However, the proportion of CaOx urolithiasis tends to be increased. CaOx urolithiasis has high incidence of recurrence after the treatment and the pathogenesis of the stone formation is uncleared. Recent studies revealed that oxalate degrader bacteria in the gastrointestinal tract has important roles in CaOx stone formation but no research has been done to study the composition and the role of microbiome in the urinary tract of dogs with CaOx urolithiasis. This …


Development Of Nanostructured Lipid Carrier Clove Oil As Anesthetic Agent In White Shrimp (Litopenaeus Vannamei), Somrudee Kaewmalun Jan 2020

Development Of Nanostructured Lipid Carrier Clove Oil As Anesthetic Agent In White Shrimp (Litopenaeus Vannamei), Somrudee Kaewmalun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Clove oil is an essential oil extracted from the dried buds and leaves of the clove plant (Syzygium aromaticum). Clove oil contains eugenol as the main active ingredient. Clove oil used as an anesthesia functions as calming effect, loss of equilibrium, non-reactivity and consciousness in lead to reduce activity, physiological stress, mortality rate, and preventing cannibalism method during handling and transportation. However, the component of clove oil is insoluble in water and must be dissolved in ethanol. So, clove oil ethanolic solution leading to toxic and irritation in animal and user. In this study, to avoid this side effect, the …


Antimicrobial Resistance In Salmonella Enterica Isolated From Dogs And Cats In Northern And Northeastern Thailand, Thanich Chantharothaiphaichit Jan 2020

Antimicrobial Resistance In Salmonella Enterica Isolated From Dogs And Cats In Northern And Northeastern Thailand, Thanich Chantharothaiphaichit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Antimicrobial resistance (AMR) is One Health issue involved in humans, animals and environment and a unified One Health approach is required to contain this problematic issue. However, data on AMR in companion animals is limited. Dogs and cats are popular pet animals and are known to carry many bacterial pathogens that are of public health importance, including Salmonella. This study aimed to identify serovars of Salmonella and phenotypically and genetically characterize AMR in Salmonella isolated from dogs and cats in Northern and Northeastern Thailand. A total of 178 S. enterica isolates from dogs (n= 159) and cats (n= 19) collected …


Recovery Of Isolated Pancreatic Isletsderived From Gut Leak-Induced Diabetes Type Ii Mouse Model, Adretta Soedarmanto Jan 2020

Recovery Of Isolated Pancreatic Isletsderived From Gut Leak-Induced Diabetes Type Ii Mouse Model, Adretta Soedarmanto

Chulalongkorn University Theses and Dissertations (Chula ETD)

Gut leak in the obese patient was known to be one of the predisposing factors causing diabetes type II. Indeed, the interleukin-10 (IL-10) plays a key role in gut mucosa homeostasis preventing the gut leak. Nevertheless, the linkage of diabetes type 2 caused by gut leak with IL-10 deficiency in the non-diabetes patient has not been investigated yet. Islets transplantation is known as the best alternative treatment for diabetes. The culture of isolated islets is still facing difficulties to preserve the morphology, viability, and functionality. Therefore, the present study was aiming to prove the concept of gut-leak on IL-10 deficient …


Effect Of Dietary Microencapsulated Organic Acid And Essential Oil On Sow Performance And Nursery Piglet Growth Performance And Fecal Bacteria, Aprilia Rizky Riadini Jan 2020

Effect Of Dietary Microencapsulated Organic Acid And Essential Oil On Sow Performance And Nursery Piglet Growth Performance And Fecal Bacteria, Aprilia Rizky Riadini

Chulalongkorn University Theses and Dissertations (Chula ETD)

The effect of feed supplementation with microencapsulated organic acids and essential oils (MOE, Porcinate+) in feed was investigated according to sow and nursery pig growth performances, and fecal bacteria population. Experiment 1: healthy three hundred and twenty sows during its late gestation period (7 days before farrowing) until the weaning phase (28 days after farrowing) were randomly divided as control group (160 sows) which was fed with basal diet, and treatment group (160 sows), which was fed with basal diets supplemented with 2 kilograms (kg) of MOE in 1 ton of feed. Each of 4 replications was composed of 40 …


Porcine Reproductive And Respiratory Syndrome Virus: Genetic Diversity, Pathogenesis And Modified-Live Vaccines, Adthakorn Madapong Jan 2020

Porcine Reproductive And Respiratory Syndrome Virus: Genetic Diversity, Pathogenesis And Modified-Live Vaccines, Adthakorn Madapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine reproductive and respiratory syndrome virus (PRRSV) is a causative agent of PRRS that reproductive failure in sows and respiratory problems in piglets is the hallmark of the disease. Recently, several PRRSV modified live vaccines (MLV) are available, and vaccine selection is a concern. Therefore, the objectives of this study were: 1) to investigate the genetic diversity of Thai PRRSV isolates during 2001-2017; 2) to evaluate the pathogenicity of Thai PRRSV isolates; 3) to determine the efficacy of PRRSV MLV against Thai field PRRSV infection and 4) to investigate the effectiveness of PRRSV MLV when administered via intramuscular (IM) and …


Genetic Characteristics Of Antimicrobial Resistance In Escherichia Coli Isolated From Pigs, Pork And Humans In Thailand And Lao Pdr Border Provinces, Chanika Pungpian Jan 2020

Genetic Characteristics Of Antimicrobial Resistance In Escherichia Coli Isolated From Pigs, Pork And Humans In Thailand And Lao Pdr Border Provinces, Chanika Pungpian

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this study were to monitor the prevalence of antimicrobial resistance (AMR) among Escherichia coli isolated from pigs, pork and humans in Thailand-Laos border areas, characterize AMR of E. coli isolated from pigs, pork and humans in Thailand-Laos border areas, and compare the resistance to last-line antimicrobials of E. coli and Salmonella isolated from pigs and pork in Thailand-Laos and Thai-Cambodia border areas. Three study projects were conducted. Project 1 demonstrated the results of phenotypic and genotypic monitoring of AMR in E. coli in pigs, pork, and humans in Thailand and Lao PDR border provinces. A total of …


Microbiological Quality Of And Antimicrobial Resistance In Commercial Probiotic Products For Food Animals In Thailand, Hoang My Tran Jan 2020

Microbiological Quality Of And Antimicrobial Resistance In Commercial Probiotic Products For Food Animals In Thailand, Hoang My Tran

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 45 commercial probiotic products for food animals were investigated for the number of viable cells, bacterial species and the presence of antimicrobial resistance (AMR) genes. All products were enumerated for viable bacterial cells of Lactobacillus, Bacillus and Enterococcus. Confirmation of species was carried out by multiplex Polymerase Chain Reaction (PCR) for Lactobacillus (n=20), Enterococcus (n=20), and Amplified Ribosomal DNA Restriction Analysis (ARDRA) for Bacillus (n=190). The presence of Clostridium species was examined by PCR. The contamination of E. coli and Salmonella was also determined. Minimal Inhibitory Concentrations (MICs) for 14 antimicrobials was examined in the bacterial isolates …


Localization Of Tilapia Tilapinevirus By In Situ Hybridization In The Brain Of Nile Tilapia (Oreochromis Niloticus), Hung Dinh Nguyen Jan 2020

Localization Of Tilapia Tilapinevirus By In Situ Hybridization In The Brain Of Nile Tilapia (Oreochromis Niloticus), Hung Dinh Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tilapia tilapinevirus or tilapia lake virus (TiLV) is an emerging virus that poses a significant negative impact on farmed tilapia globally. Abnormal behaviours such as lethargy, loss of appetite, erratic swimming, stop schooling have been recorded during disease occurrence, which is possibly linked to brain activities. Previous studies described detection of the virus in multiple organs of infected fish; however, little is known about the in-depth localization of the virus in the central nervous system. Herein, we determined the distribution of TiLV and histopathological alterations in the entire brain of Nile tilapia experimentally infected fish. By in situ hybridization (ISH) …


Detection Of Indicator And Pathogenic Bacteria In Raw Vegetables And Fruits Sold In Fresh Markets And Supermarkets, Bangkok, Thailand, Jutanat Srisamran Jan 2020

Detection Of Indicator And Pathogenic Bacteria In Raw Vegetables And Fruits Sold In Fresh Markets And Supermarkets, Bangkok, Thailand, Jutanat Srisamran

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to compare the concentrations of fecal coliforms and Escherichia coli, and the contamination of Salmonella, Shigella, E. coli O157: H7 and Listeria monocytogenes in raw vegetables and fruits between fresh markets and supermarkets in Bangkok, Thailand. A total of 405 produce samples, consisting of sweet basil, spring onion, coriander, cabbage, lettuce, cucumber and tomato, were randomly collected from seven fresh markets (n=203) and 29 supermarkets (n=202) in seven districts of Bangkok between June 2018 and July 2019. T-test and one-way ANOVA were used to compare the concentrations of fecal coliforms and E. coli (log10MPN/g) between fresh markets …


A Study Of Using Corncob, Aspen Shaving, Dried Water Hyacinth And Banana Midrib As Bedding For Laboratory Mice, Nantaporn Maytayapirom Jan 2020

A Study Of Using Corncob, Aspen Shaving, Dried Water Hyacinth And Banana Midrib As Bedding For Laboratory Mice, Nantaporn Maytayapirom

Chulalongkorn University Theses and Dissertations (Chula ETD)

A study of using corncob, aspen shaving, dried water hyacinth and banana midrib as bedding for laboratory mice. The objective of this study was to evaluate the physical characteristics (i.e., absorbency and surface moisture), the physiological effects and intracage ammonia level of 4 types of beddings in laboratory mice. This study was divided into two parts. In the first part, the fluid absorption properties of these four beddings were studied using measurement of liquid absorption and the surface moisture. The results showed that the volumetric absorbency of aspen shaving was significantly higher than other beddings. While, the surface moisture showed …


Targeted Surveillance Of Potential Zoonotic Respiratory And Enteric Viruses In Dogs In Bangkok, Thailand, Kamonpan Charoenkul Jan 2020

Targeted Surveillance Of Potential Zoonotic Respiratory And Enteric Viruses In Dogs In Bangkok, Thailand, Kamonpan Charoenkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human-dog interface poses a risk of transmission and spread of zoonotic viruses. The objective of the thesis entitled is to survey and genetic characterize zoonotic viruses in dogs and humans who have high-risk occupations and in close contact with dogs. This thesis contains 8 topics. The results of the first to third topics were the surveillance of respiratory viruses in dogs including Canine Parainfluenza type 5 (CPIV-5), Canine influenza virus (CIV) and Coronavirus (CoV). The nasal swab samples were collected from dogs with respiratory signs during 2015-2018. The results showed that the occurrence of CPIV-5, CIV and CoV were 5.6% …


The Study Of Changes In Gene Regulation Of Serotonin Signaling Pathway And Circulating Serotonin Concentration In Dogs With Pulmonary Hypertension Due To Degenerative Mitral Valve Disease, Nattawan Tangmahakul Jan 2020

The Study Of Changes In Gene Regulation Of Serotonin Signaling Pathway And Circulating Serotonin Concentration In Dogs With Pulmonary Hypertension Due To Degenerative Mitral Valve Disease, Nattawan Tangmahakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Pulmonary hypertension (PH) is an abnormal increase in pulmonary vascular pressure. In dogs, PH is commonly found secondary to degenerative mitral valve disease (DMVD), especially in small breed dogs. Serotonin, a biogenic amine playing an essential role in both physiology and abnormalities of several organs, is a vasoactive substance that has been one of the suspicious mediators for the development of PH. Both local and circulating effects of serotonin have been investigated to discover the involvement of serotonin and the pathogenesis of PH in both humans and animals. The present study aimed to investigate the local serotonin signaling in lung …


Comparison Of Laser Class 1m And Class 4 Applications In Canine Cranial Cruciate Ligament Rupture Evaluated By Clinical Outcomes And Ultrasonography, Noppakrit Ekakaraworapong Jan 2020

Comparison Of Laser Class 1m And Class 4 Applications In Canine Cranial Cruciate Ligament Rupture Evaluated By Clinical Outcomes And Ultrasonography, Noppakrit Ekakaraworapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Nineteen dogs diagnosed of cranial cruciate ligament rupture were included in this study before TPLO was performed. They were classified into 3 groups which consisted of control, low-level laser therapy class 1M and low-level laser therapy class 4 groups. The number of each group were 4, 8 and 7 dogs, respectively, and the duration of treatment protocol was 4 weeks consecutively. On the first day of the study, dogs were examined and assessed of the clinical signs such as lameness score, synovial fluid analysis, canine brief pain inventory questionnaire (CBPI) and ultrasonographic score. In ultrasonographic monitoring, ultrasonographic scores on synovial …


Efficiency And Clinical Outcomes Of Platlet-Rich Plasma Therapy On Caninecoxofemoral Osteoarthritis, Patama Arunrattanakul Jan 2020

Efficiency And Clinical Outcomes Of Platlet-Rich Plasma Therapy On Caninecoxofemoral Osteoarthritis, Patama Arunrattanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study was to find out for the optimal technique to prepare plasma with highest platelet concentration in platelet rich plasma (PRP), as well as determine the result of autologous PRP from the dogs with osteoarthritis(OA) condition. It was shown that the PRP could reduce inflammation, cell regeneration, and enhance angiogenesis to support the injured tissue. This study was composed of two phases; the first phase was aimed to find the best centrifugation speed and time that could be able to produce the plasma with the highest platelet concentration by conducting the experiment from a total of …


Surveillance Of Coronaviruses In Fruit Bats (Pteropus Lylei) Of Thailand, Pichamon Moolthep Jan 2020

Surveillance Of Coronaviruses In Fruit Bats (Pteropus Lylei) Of Thailand, Pichamon Moolthep

Chulalongkorn University Theses and Dissertations (Chula ETD)

Coronavirus (CoV) is an enveloped single positive-stranded RNA virus CoV can cause enteric and respiratory diseases in mammals, avian and human. Bats are the natural reservoir of CoVs and distribute all over Thailand. Sample collection was performed in two bat colonies in Ayutthaya and Saraburi provinces during March 2018 - February 2019. Total 1,487 samples were collected from 730 bats. The bat species identification via morphological measurement and cytochrome B detection showed that bats in both colonies are Lyle's flying fox (Pteropus lylei). The results of CoV detection showed that 4.30% of samples were positive to CoV. Phylogenetic analysis showed …