Medicine and Health Sciences Commons^™

Characterizing The Metabolic Intensity And Cardiovascular Demands Of Walking Football In Southeast Asian Women, D. P. Heil, Robert U. Newton, Dee Dee Ayra Salle

Research outputs 2014 to 2021

Given that the recent rise in obesity rates throughout Southeast Asia is disproportionately driven by women, part of the regional solution may be to encourage more habitual physical activity within this population. Taking advantage of the regional popularity of walking football, this study sought to characterize the cardiovascular demands and metabolic intensity of Southeast Asian women competing in walking football matches to determine the sports’ suitability for promoting physical health. It was hypothesized that both cardiovascular and metabolic intensity measures (≥65% HR% and ≥3.0 METs, respectively) would meet or exceed established thresholds for improving fitness and health. Methods: Women’s teams …

Movement Economy In Soccer: Current Data And Limitations, Filippo Dolci, Nicolas H. Hart, Andrew Kilding, Paola Chivers, Ben Piggott, Tania Spiteri

Research outputs 2014 to 2021

Soccer is an intermittent team-sport, where performance is determined by a myriad of psychological, technical, tactical, and physical factors. Among the physical factors, endurance appears to play a key role into counteracting the fatigue-related reduction in running performance observed during soccer matches. One physiological determinant of endurance is movement economy, which represents the aerobic energy cost to exercise at a given submaximal velocity. While the role of movement economy has been extensively examined in endurance athletes, it has received little attention in soccer players, but may be an important factor, given the prolonged demands of match play. For this reason, …

Strategies To Address The Complex Challenge Of Improving Regional And Remote Children’S Fruit And Vegetable Consumption, Stephanie L. Godrich, Christina R. Davies, Jill Darby, Amanda Devine

Research outputs 2014 to 2021

Fruit and vegetables (F&V) are imperative for good health, yet less than one per cent of Australian children consume these food groups in sufficient quantities. As guided by Social Cognitive Theory (SCT), this paper aimed to: (i) understand key informant perspectives of the amount, types and quality of F&V consumed by rural and remote Western Australian (WA) children; and, (ii) determine strategies that could increase F&V consumption among rural and remote WA children. This qualitative study included 20 semi-structured interviews with health, school/youth and food supply workers, focusing on topics including: quantity and type of F&V consumed and strategies to …

Weight Loss Strategies In Combat Sports And Concerning Habits In Mixed Martial Arts, Oliver R. Barley, Dale W. Chapman, Chris Abbiss

Research outputs 2014 to 2021

Context:

Combat sports are typically divided into weight classes, and body-mass manipulation to reach a weight class is commonplace. Previous research suggests that weight loss practices in mixed martial arts (MMA) may be more extreme than in other combat sports.

Purpose:

To investigate the magnitude of weight loss and the prevalence of weight loss strategies in different combat sports.

Methods:

Competitors (N = 637) from Brazilian jiu-jitsu, boxing, judo, MMA, Muay Thai/kickboxing, taekwondo, and wrestling completed an online questionnaire seeking information regarding their weight loss practices.

Results:

Body-mass manipulation was commonly undertaken by all combat-sport athletes, …

Bridging The Gap Between Clinicians And Fitness Professionals, Rachele Pojednic, Amy Bantham, Fred Arnstein, Mary A. Kennedy, Edward Phillips

Research outputs 2014 to 2021

Objective Health clubs (HC) and personal trainers (PT) are traditional outlets for the promotion of physical activity (PA) and exercise programming. As physicians are increasingly being called on to write exercise prescriptions for their patients, this study sought to investigate the level of integration between the healthcare and fitness systems.

Design An internet study was designed with five domains to understand physicians’: (1) overall perception of HC, (2) appropriateness and recommendation of HC and PT to their patients, (3) attitude regarding specific aspects of HC, (4) support of patient participation in HC sponsored exercise and (5) elements of HC that …

Optimum Power Loads For Elite Boxers: Case Study With The Brazilian National Olympic Team, Irineu Loturco, Chris Bishop, Rodrigo Ramirez-Campillo, Felipe Romano, Mateus Alves, Lucas A. Pereira, Michael Mcguigan

Research outputs 2014 to 2021

The purpose of this case study was to examine the effects of a resistance-training program based on the optimum power loads (OPL) method on neuromuscular performance of Olympic boxing athletes during preparation for the Rio-2016 Olympic Games. Twelve elite amateur boxers from the Brazilian National Olympic Team participated in this study. Athletes were assessed at four time-points, over two consecutive competitive seasons. In the first season (considered as “control period”), the athletes executed a non-controlled strength-power training program for 10 weeks. In the second season (a seven-week experimental period), the elite boxers performed 14 power-oriented training sessions, comprising bench press …

The Weakening Of Kin Ties: Exploring The Need For Life-World Led Interventions, Gert Schout, Gideon De Jong

Research outputs 2014 to 2021

The protective features that families and wider social relationships can have are required to meet the demands of life in contemporary Western societies. Choice and detraditionalization, however; impede this source of solidarity. Family Group Conferencing (FGC) and other life-world led interventions have the potential to strengthen primary groups. This paper explores the need for such a social intervention, using insights from sociological and philosophical theories and empirical findings from a case study of the research project ‘FGC in mental health’. This need is understandable considering the weakening of kin ties, the poor qualities of state agencies to mobilise self-care and …

Beyond Factor Analysis: Multidimensionality And The Parkinson’S Disease Sleep Scale-Revised, Maria E. Pushpanathan, Andrea M. Loftus, Natalie Gasson, Meghan G. Thomas, Caitlin F. Timms, Michelle Olaithe, Romola S. Bucks

Research outputs 2014 to 2021

Many studies have sought to describe the relationship between sleep disturbance and cognition in Parkinson’s disease (PD). The Parkinson’s Disease Sleep Scale (PDSS) and its variants (the Parkinson’s disease Sleep Scale-Revised; PDSS-R, and the Parkinson’s Disease Sleep Scale-2; PDSS-2) quantify a range of symptoms impacting sleep in only 15 items. However, data from these scales may be problematic as included items have considerable conceptual breadth, and there may be overlap in the constructs assessed. Multidimensional measurement models, accounting for the tendency for items to measure multiple constructs, may be useful more accurately to model variance than traditional confirmatory factor analysis. …

Flavonoid-Rich Apple Improves Endothelial Function In Individuals At Risk For Cardiovascular Disease: A Randomized Controlled Clinical Trial, Nicola P. Bondonno, Catherine P. Bondonno, Lauren C. Blekkenhorst, Michael J. Considine, Ghassan Maghzal, Roland Stocker, Richard J. Woodman, Natalie C. Ward, Jonathan M. Hodgson, Kevin D. Croft

Research outputs 2014 to 2021

Scope

The cardioprotective effects of apples are primarily attributed to flavonoids, found predominantly in the skin. This study aimed to determine if acute and/or chronic (4 weeks) ingestion of flavonoid-rich apples improves endothelial function, blood pressure (BP), and arterial stiffness in individuals at risk for cardiovascular diseases (CVD).

Methods and results

In this randomized, controlled cross-over trial, acute and 4 week intake of apple with skin (high flavonoid apple, HFA) is compared to intake of apple flesh only (low flavonoid apple, LFA) in 30 participants. The primary outcome is endothelial function assessed using flow-mediated dilation (FMD) of the brachial artery, …

Interpreting Signal Amplitudes In Surface Electromyography Studies In Sport And Rehabilitation Sciences, Andrew D. Vigotsky, Israel Halperin, Gregory J. Lehman, Gabriel S. Trajano, Taian M. Vieira

Research outputs 2014 to 2021

Surface electromyography (sEMG) is a popular research tool in sport and rehabilitation sciences. Common study designs include the comparison of sEMG amplitudes collected from different muscles as participants perform various exercises and techniques under different loads. Based on such comparisons, researchers attempt to draw conclusions concerning the neuro- and electrophysiological underpinning of force production and hypothesize about possible longitudinal adaptations, such as strength and hypertrophy. However, such conclusions are frequently unsubstantiated and unwarranted. Hence, the goal of this review is to discuss what can and cannot be inferred from comparative research designs as it pertains to both the acute and …

Barriers To Promoting Mobility In Hospitalized Older Adults, Gordana Dermody, Christine R. Kovach

Research outputs 2014 to 2021

Hospitalized older adults who do not receive sufficient mobility are more likely to sustain negative health outcomes, including higher rates of mortality and institutionalization. Accordingly, the purpose of the current secondary data analysis was to examine the nurse-promoted mobility of hospitalized older adults and the association between nurses’ barriers and nurse-promoted mobility. In addition, the relationship among patient severity of illness, proxy levels for function, and nurse-promoted mobility was examined. The final study sample included 61 nurses working in medical units caring for a total of 77 older adults. Findings suggest nurse knowledge gaps and attitude barriers could potentially influence …

Kibra Is Associated With Accelerated Cognitive Decline And Hippocampal Atrophy In Apoe Ε4-Positive Cognitively Normal Adults With High Aβ-Amyloid Burden, Tenielle Porter, Samantha Burnham, Vincent Doré, Greg Savage, Pierrick Bourgeat, Kimberly Begemann, Lidija Milicic, David Ames, Ashley Bush, Paul Maruff, Colin Masters, Christopher Rowe, Stephanie Rainey-Smith, Ralph Martins, David Groth, Guiseppe Verdile, Victor Villemagne, Simon Laws

Research outputs 2014 to 2021

A single nucleotide polymorphism, rs17070145, in the KIdney and BRAin expressed protein (KIBRA) gene has been associated with cognition and hippocampal volume in cognitively normal (CN) individuals. However, the impact of rs17070145 on longitudinal cognitive decline and hippocampal atrophy in CN adults at greatest risk of developing Alzheimer’s disease is unknown. We investigated the impact rs17070145 has on the rate of cognitive decline and hippocampal atrophy over six years in 602 CN adults, with known brain Aβ-amyloid levels and whether there is an interactive effect with APOE genotype. We reveal that whilst limited independent effects of KIBRA genotype …

Genetic Variation In Aquaporin-4 Moderates The Relationship Between Sleep And Brain Aβ-Amyloid Burden, Stephanie Rainey-Smith, Gavin N. Mazzucchelli, Victor L. Villemagne, Belinda M. Brown, Tenielle Porter, Michael Weinborn, Romola Bucks, Lidija Milicic, Hamid R. Sohrabi, Kevin Taddei, David J. Ames, Paul T. Maruff, Colin L. Masters, Christopher C. Rowe, Olivier Salvado, Ralph N. Martins, Simon M. Laws

Research outputs 2014 to 2021

The glymphatic system is postulated to be a mechanism of brain Aβ-Amyloid clearance and to be most effective during sleep. Ablation of the astrocytic end-feet expressed water-channel protein, Aquaporin-4, in mice, results in impairment of this clearance mechanism and increased brain Aβ-Amyloid deposition, suggesting that Aquaporin-4 plays a pivotal role in glymphatic function. Currently there is a paucity of literature regarding the impact of AQP4 genetic variation on sleep, brain Aβ-Amyloid burden and their relationship to each other in humans. To address this a cross-sectional observational study was undertaken in cognitively normal older adults from the Australian Imaging, Biomarkers and …

In The Shadow Of The Ivory Tower: Experiences Of Midwives And Nurses Undertaking Phds, Sadie Geraghty, Kim Oliver

Research outputs 2014 to 2021

Aims: The aims of this study were to gain an understanding of the experiences of nurses and midwives enrolled in a PhD, explore any barriers that PhD students encounter whilst completing the degree, and develop recommendations for consideration in formulating support strategies to encourage completion for nurses and midwives enrolled on a PhD degree. Background: It is important to understand what is happening at doctoral level education for nurses and midwives, and how those enrolled on PhD courses can successfully complete their studies, transition learning, and acquire the ability to undertake research into the clinical environments. Design: The criteria for …

Rpe Vs. Percentage 1rm Loading In Periodized Programs Matched For Sets And Repetitions, Eric R. Helms, Ryan K. Byrnes, Daniel M. Cooke, Michael H. Haischer, Joseph P. Carzoli, Trevor K. Johnson, Matt R. Cross, John B. Cronin, Adam G. Storey, Michael C. Zourdos

Research outputs 2014 to 2021

Purpose: To investigate differences between rating of perceived exertion (RPE) and percentage one-repetition maximum (1RM) load assignment in resistance-trained males (19-35 years) performing protocols with matched sets and repetitions differentiated by load-assignment. Methods: Participants performed squats then bench press 3x/weeks in a daily undulating format over 8-weeks. Participants were counterbalanced by pre-test 1RM then assigned to percentage 1RM (1RMG, n = 11); load-assignment via percentage 1RMs, or RPE groups (RPEG, n = 10); participant-selected loads to reach target RPE ranges. Ultrasonography determined pre and post-test pectoralis (PMT), and vastus lateralis muscle thickness at 50 (VLMT50) and 70% (VLMT70) femur-length. Results: …

Association Of Obstructive Sleep Apnea With Hypertension: A Systematic Review And Meta-Analysis, Haifeng Hou, Yange Zhao, Xiaotong Xue, Jian Ding, Wei Jia Xing, Wei Wang

Research outputs 2014 to 2021

Results: Twenty-six studies with 51 623 participants (28 314 men, 23 309 women; mean age 51.8 years) met inclusion criteria and were included in this study. Among them, six studies showed a significant association between OSA and resistant hypertension (pooled OR = 2.842, 95% CI = 1.703-3.980, P < 0.05). Meanwhile, the combination of 20 original studies on the association of OSA with essential hypertension also presented significant results with the pooled ORs of 1.184 (95% CI = 1.093-1.274, P < 0.05) for mild OSA, 1.316 (95% CI = 1.197-1.433, P < 0.05) for moderate OSA and 1.561 (95% CI = 1.287-1.835, P < 0.05) for severe OSA.

Conclusions: Our findings indicated that OSA is related to an increased risk of resistant hypertension. Mild, moderate and severe OSA are associated essential hypertension, as well a dose-response manner relationship is manifested. The associations are relatively stronger among Caucasians and male OSA patients.

Background: Obstructive sleep apnea (OSA) is a sleep disorder …

Metabolic And Fatigue Profiles Are Comparable Between Prepubertal Children And Well-Trained Adult Endurance Athletes, Anthony Birat, Pierre Bourdier, Enzo Piponnier, Anthony J. Blazevich, Hugo Maciejewski, Pascale Duche, Sebastien Ratel

Research outputs 2014 to 2021

The aim of this study was to determine whether prepubertal children are metabolically comparable to well-trained adult endurance athletes and if this translates into similar fatigue rates during high-intensity exercise in both populations. On two different occasions, 12 prepubertal boys (10.5 ± 1.1 y), 12 untrained men (21.2 ± 1.5 y), and 13 endurance male athletes (21.5 ± 2.7 y) completed an incremental test to determine the power output at VO_2max (PVO_2max) and a Wingate test to evaluate the maximal anaerobic power (P_max) and relative decrement in power output (i.e., the fatigue index, FI). Furthermore, …

The Role Of Adult Day Services In Supporting The Occupational Participation Of People With Dementia And Their Carers: An Integrative Review, Janice Du Preez, J. L. Millsteed, Ruth Marquis, Janet Richmond

Research outputs 2014 to 2021

The increasing numbers of people with dementia places considerable stress on health and aged care services and has resulted in the development of community adult day services. Aim: The aim of this integrative review is to determine the extent to which these services support the occupational participation of people with dementia, and how they impact their primary carers. Method: The mixed-methods appraisal tool (MMAT) was used to identify relevant studies in the period 2011–2016. Results: Nine databases were searched and yielded 16 articles with a variety of research designs for inclusion in the review. Conclusions: Findings …

Geographical Variations In The Clinical Management Of Colorectal Cancer In Australia: A Systematic Review, Fiona Crawford-Williams, Sonja March, Michael Ireland, Arlen Rowe, Belinda Goodwin, Melissa Hyde, Suzanne K. Chambers, Joanne Aitken, Jeff Dunn

Research outputs 2014 to 2021

Background: In Australia, cancer survival is significantly lower in non-metropolitan compared to metropolitan areas. Our objective was to evaluate the evidence on geographical variations in the clinical management and treatment of colorectal cancer (CRC).

Methods: A systematic review of published and gray literature was conducted. Five databases (CINAHL, PubMed, Embase, ProQuest, and Informit) were searched for articles published in English from 1990 to 2018. Studies were included if they assessed differences in clinical management according to geographical location; focused on CRC patients; and were conducted in Australia. Included studies were critically appraised using a modified Newcastle–Ottawa Scale. PRISMA systematic review …

Properties Of Traditional Bamboo Carrying Poles Have Implications For User Interactions, Ryan T. Schroeder, James L. Croft, Giang D. Ngo, John E. A. Bertram

Research outputs 2014 to 2021

Compliant bamboo poles have long been used for load carriage in Asian cultures. Although this custom differs from Western conventions of rigid body attachments (e.g. backpack), potential benefits include reduced peak shoulder forces as well as metabolic transport cost savings. Evidence that carrying a flexible pole benefits locomotion remains mixed, perhaps in part because the properties of pole design (e.g. bamboo material, structural geometry, etc.) have largely been neglected. These properties influence vibrational forces and consequently, the energy required by the user to manage the oscillations. We collected authentic bamboo poles from northern Vietnam and characterized their design parameters. Four …

An Overview Of Technical Considerations When Using Quantitative Real-Time Pcr Analysis Of Gene Expression In Human Exercise Research, Jujiao Kuang, Xu Yan, Amanda Genders, Cesare Granata, David J. Bishop

Research outputs 2014 to 2021

Gene expression analysis by quantitative PCR in skeletal muscle is routine in exercise studies. The reproducibility and reliability of the data fundamentally depend on how the experiments are performed and interpreted. Despite the popularity of the assay, there is a considerable variation in experimental protocols and data analyses from different laboratories, and there is a lack of consistency of proper quality control steps throughout the assay. In this study, we present a number of experiments on various steps of quantitative PCR workflow, and demonstrate how to perform a quantitative PCR experiment with human skeletal muscle samples in an exercise study. …

Ischemic Stroke Is Associated With The Pro-Inflammatory Potential Of N-Glycosylated Immunoglobulin G, Di Liu, Zhongyao Zhao, Anxin Wang, Siqi Ge, Hao Wang, Xiaoyu Zhang, Qi Sun, Weijie Cao, Ming Sun, Lijuan Wu, Manshu Song, Yong Zhou, Wei Wang, Youxin Wang

Research outputs 2014 to 2021

Background

Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes. Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension.

Methods

The study aimed to investigate the association between IgG N-glycosylation and IS in a Chinese population. IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography.

Results

Eleven initial glycans and 10 …

Intrarater Reliability And Agreement Of The Physioflow Bioimpedance Cardiography Device During Rest, Moderate And High-Intensity Exercise, Nicole Gordon, Chris R. Abbiss, Andrew J. Maiorana, Kieran J. Marston, Jeremiah J. Peiffer

Research outputs 2014 to 2021

The PhysioFlow bioimpedance cardiography device provides key measures of central systolic and diastolic and peripheral vascular function. Many of these variables have not been assessed for intrarater reliability and agreement during rest, submaximal exercise and high-intensity interval exercise. Twenty healthy adults (age: 26±4 years) completed two identical trials beginning with five minutes of rest followed by two 5-minute submaximal cycling bouts at 50% and 70% of peak power output. Subjects then completed ten 30-second cycling intervals at 90% of peak power output interspersed with 60 s of passive recovery. Bioimpedance cardiography (PhysioFlow; Manatec Biomedical, France) monitored heart rate, stroke volume, …

The Effects Of Acute And Chronic Sprint-Interval Training On Cytokine Responses Are Independent Of Prior Caffeine Intake, Guilherme Ferreira, Leandro Felippe, Rômulo Bertuzzi, David Bishop, Emiliano Barreto, Fernando De-Oliveira, Adriano Lima-Silva

Research outputs 2014 to 2021

We examined the effect of acute and chronic sprint interval training (SIT), with or without prior caffeine intake, on levels of exercise-induced inflammatory plasma cytokines [interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-α]. Twenty physically-active men ingested either a placebo (n = 10) or caffeine (n = 10) 1 h before each SIT session(13-s × 30-s sprint/15 s of rest) during six training sessions (2 weeks). The early (before, immediately after, and 45 min after the exercise) and late (24 and 48 h after the exercise) cytokine and creatine kinase (CK) responses were analyzed for the first and last …

Can Exercise Delay Transition To Active Therapy In Men With Low-Grade Prostate Cancer? A Multicentre Randomised Controlled Trial, Daniel A. Galvao, Dickon Hayne, Mark Frydenberg, Suzanne K. Chambers, Dennis R. Taaffe, Nigel Spry, Paul A. Scuffham, Robert S. Ware, Nicolas H. Hart, Robert U. Newton

Research outputs 2014 to 2021

Introduction

Active surveillance is a strategy for managing low-risk, localised prostate cancer, where men are observed with serial prostate-specific antigen assessments to identify signs of disease progression. Currently, there are no strategies to support active surveillance compliance nor are there interventions that can prevent or slow disease progression, ultimately delaying transition to active treatment before it is clinically required. Recently, we proposed that exercise may have a therapeutic potential in delaying the need for active treatment in men on active surveillance.

Methods and analysis

A single-blinded, two arm, multicentre randomised controlled trial will be undertaken with 168 patients randomly allocated …

A Diagnostic Autoantibody Signature For Primary Cutaneous Melanoma, Pauline Zaenker, Johnny Lo, Robert L. Pearce, Phillip Cantwell, Lester Cowell, Mark Lee, Christopher Quirk, Henry Law, Elin S. Gray, Mel R. Ziman Dr

Research outputs 2014 to 2021

Melanoma is an aggressive form of skin cancer that is curable by surgical excision in the majority of cases, if detected at an early stage. To improve early stage melanoma detection, the development of a highly sensitive diagnostic test is of utmost importance. Here we aimed to identify antibodies to a panel of tumour associated antigens that can differentiate primary melanoma patients and healthy individuals. A total of 245 sera from primary melanoma patients and healthy volunteers were screened against a high-throughput microarray platform containing 1627 functional proteins. Following rigorous statistical analysis, we identified a combination of 10 autoantibody biomarkers …

Metabolic Syndrome Among Schizophrenic Patients: A Comparative Cross-Sectional Study In The Middle Belt Of Ghana, Angela Owusu-Ansah, Anto Berko Panyin, Christian Obirikorang, Christian Agyare, Emmanuel Acheampong, Simon Kwofie, Enoch Odame Anto, Emmanuella Nsenbah Batu

Research outputs 2014 to 2021

The study determined the prevalence ofMetS in patients with schizophrenia at the Psychiatric Unit of the Komfo Anokye Teaching Hospital (KATH), Kumasi, Ghana. This comparative cross-sectional study recruited 348 schizophrenic patients comprising 236 antipsychotic-treated and 112 newly diagnosed treatment-na¨ıve patients. The MetS prevalence was assessed based on World Health Organization (WHO), International Diabetes Federation (IDF), and the National Cholesterol Education Programme, Adult Treatment Panel III (NCEP ATP III) criteria. The overall prevalence of MetS was 14.1%, 20.4%, and 23.6% using NCEP ATP III, WHO, and IDF criteria, respectively, compared to 7.8%, 3.9%, and 2.2% reported in the generalGhanaian population.The prevalence …

What Drives Food Insecurity In Western Australia? How The Perceptions Of People At Risk Differ To Those Of Stakeholders, Lucy M. Butcher, Maria M. Ryan, Therese A. O'Sullivan, Johnny Lo, Amanda Devine

Research outputs 2014 to 2021

Food insecurity is considered a “wicked” problem due to the highly complex and at times undefined casual factors. Although many stakeholders are working to address the problem, a possible divergence exists between their views on food insecurity and those of the people who are actually experiencing the problem. The purpose of this study was to investigate whether there was a difference between the opinions of those “at risk” and stakeholders. A total of seven focus groups (two stakeholder groups n = 10, five “at-risk” groups n = 34) and three interviews (stakeholders n = 3) were conducted to ascertain perceptions. …

Development Of An Australian Nursing Critical Thinking Tool Using A Delphi Process, Elisabeth R. Jacob, Christine M. Duffield, Darren Jacob

Research outputs 2014 to 2021

Aim: To develop a critical thinking assessment tool for Australian undergraduate nurses. Background: Critical thinking is an important skill but difficult to assess in nursing practice. There are often many responses a nurse can make to a clinical problem or situation. Some responses are more correct than others and these decisions have an impact on a patient's care and safety. Differences in a response can relate to the depth of knowledge, experience and critical thinking ability of the individual nurse. Design: This study used a Delphi process to develop five clinical case studies together with the most appropriate clinical responses …

Efficient Production Of A Mature And Functional Gamma Secretase Protease, Imran Khan, Sudarsan Krishnaswamy, Miheer Sabale, David M. Groth, Linda Wijaya, Michael Morici, Imre Berger, Christiane Schaffitzel, Paul E. Fraser, Ralph N. Martins, Giuseppe Verdile

Research outputs 2014 to 2021

Baculoviral protein expression in insect cells has been previously used to generate large quantities of a protein of interest for subsequent use in biochemical and structural analyses. The MultiBac baculovirus protein expression system has enabled, the use of a single baculovirus to reconstitute a protein complex of interest, resulting in a larger protein yield. Using this system, we aimed to reconstruct the gamma (γ)-secretase complex, a multiprotein enzyme complex essential for the production of amyloid-β (Aβ) protein. A MultiBac vector containing all components of the γ-secretase complex was generated and expression was observed for all components. The complex was active …