Biology Commons^™

Development Of A Novel Edna Sampling Method For Macroinvertebrate Species Of Greatest Conservation Need (Sgcn) In Texas, James York

Biology Theses

Environmental DNA (eDNA) sampling is a new sampling technique that allows for non-invasive sampling of target species or the characterization of entire communities based on barcoding genes. The goal of this study was to sequence common barcoding genes such as 28S rRNA and Cytochrome C Oxidase I (COI), and then develop species-specific primers for eight macroinvertebrates listed as Species of Greatest Conservation Need (SGCN) in Texas. After sampling for the target species, five were not found, all of which were in the Order Trichoptera (caddisflies). A sixth target species, Isoperla sagittata, was found to have only one gap and …

Investigating Scube3 Nuclear Localization In Presence Of Doxorubicin Treatment In Triple Negative Breast Cancer Cells, Lillian Ergle

Merge

Signal peptide-CUB-EGF-like domain-containing protein 3 (SCUBE3) is a glycosylated secreted protein. Prior to its secretion, SCUBE3 localizes in the cytoplasm. We observed that SCUBE3 protein trans-localized to the nucleus following Doxorubicin (DOX) treatment. DOX is one of the strongest chemotherapeutic agents and the first-line drug used in breast cancer treatment. SCUBE3 structural analysis showed that it lacks a DNA binding domain. Based on this observation and other preliminary data, we hypothesized that nuclear SCUBE3 protein promotes the survival of cells against Doxorubicin treatment. To investigate this hypothesis, we made a wild-type construct and three constructs with mutated nuclear localization sequences …

Documentation Of Hadrosaurid Humeri From The Liscomb Bonebed, North Slope, Alaska Prior To Histological Sectioning, Makenna Galvin

Student Research Poster Presentations 2024

Proper documentation of research specimens is a crucial component that must be completed prior to histological sectioning. Since specimens are unique and irreplaceable, documentation provides information that may later be unobtainable after sectioning, due to the inherent nature of the technique having to permanently alter the specimen. The hadrosaurid dinosaur, the Edmontosaurus, has an abundant fossil record, which can be partly attributed to being gregarious, and therefore preserves multiple monodominant bonebeds and several dozen associated skeletons across North America. This makes Edmontosaurus an ideal taxon to use for life history studies. However, previous Edmontosaurus bonebeds have only preserved specimens of …

The Effects Of Bisphenol S On Glial Cells Of The Peripheral Nervous System, Madeline Solomon, Rachael Sennett, Angela Asirvatham

Student Research Poster Presentations 2024

Schwann cells are glial cells in the peripheral nervous system that maintain nerve function and myelination. Peripheral nerve myelination is dependent on intact Schwann cell proliferation. Schwann cell proliferation can be adversely affected by environmental pollutants such as Bisphenol A (BPA). BPA is an endocrine-disrupting chemical found in plastic water bottles, receipt paper, containers, and many everyday products. Studies on BPA exposure showed that it stimulated cellular proliferation in neural stem cells. A commonly used manufactured derivative of BPA, Bisphenol S (BPS), has posed similar concerns. Previous research has found that BPS promotes the proliferation of MCF-7 breast cancer cells …

The Effects Of Bpf On Rt4 Schwannoma Cancer Cells, Rachael Sennett, Madeline Solomon, Angela Asirvatham

Student Research Poster Presentations 2024

Bisphenol A is a harmful compound common in plastics that causes negative health and environmental effects. Due to these effects, new guidelines have been made to limit exposure to BPA. However, modified versions of these compounds, including Bisphenol-F, are being used as a replacement. Exposure to BPF, which is comparable in structure to BPA, has similar effects as BPA on many organ systems with some being unknown. As there is not much known about BPF, especially in the peripheral nervous system, the aim of this research is to analyze proliferation rates in BPF-treated Schwann cells. It was hypothesized that with …

Nutritional Convergence In Plants Growing On Gypsum Soils In Two Distinct Climatic Regions, Clare T. Muller, Andreu Cera, Sara Palacio, Michael J. Moore, Pablo Tejero, Juan F. Mota, Rebecca E. Drenovsky

2024 Faculty Bibliography

Background and Aims

Soil endemics have long fascinated botanists owing to the insights they can provide about plant ecology and evolution. Often, these species have unique foliar nutrient composition patterns that reflect potential physiological adaptations to these harsh soil types. However, understanding global nutritional patterns to unique soil types can be complicated by the influence of recent and ancient evolutionary events. Our goal was to understand whether plant specialization to unique soils is a stronger determinant of nutrient composition of plants than climate or evolutionary constraints. Methods

We worked on gypsum soils. We analysed whole-plant nutrient composition (leaves, stems, coarse …

Description Of Six New Cyanobacterial Species From Soil Biocrusts On San Nicolas Island, California, In Three Genera Previously Restricted To Brazil, Brian Jusko, Jeffrey R. Johansen

2024 Faculty Bibliography

As the taxonomic knowledge of cyanobacteria from terrestrial environments increases, it remains important to analyze biodiversity in areas that have been understudied to fully understand global and endemic diversity. This study was completed as part of a larger algal biodiversity study of the soil biocrusts of San Nicholas Island, California, USA. Among the taxa isolated were several new species in three genera (Atlanticothrix, Pycnacronema, and Konicacronema) which were described from, and previously restricted to, Brazil. New taxa are described herein using a polyphasic approach to cyanobacterial taxonomy that considers morphological, molecular, ecological, and biogeographical factors. Morphological data corroborated by molecular …

The Impact Of Isolation From Other Members Of The Greater Bamboo Lemur (Prolemur Simus) Species On The Behavior Of A Lone Greater Bamboo Lemur In Ranomafana National Park, Marissa N. Mccandless, Alex Casamassima, Sophie Thomas

Undergraduate Research (Journal)

Across Madagascar, many lemur species are declining in number due to deforestation perpetuated by slash and burn agriculture. Ranomafana National Park hosts 12 unique lemur species, one of which has a local population of just a single individual- the Greater Bamboo lemur (Prolemur simus). Being extremely social beings, we theorize that isolation from members of one’s species will change behavioral patterns. To test this hypothesis, we used focal animal sampling to record the behavior of the Greater Bamboo lemur for about 40 hours over a five day period. We compared our results to behavioral data collected of lemurs in a …

A Comparative Study Of Biocrusts On Gypsum And Non-Gypsum Soils In The Northern Chihuahuan And Eastern Mojave Deserts, Usa: Biocrust Mosses Respond To Soil, Environmental, And Climatic Conditions, Katelyn Gobbie

Masters Theses

Biological soil crust communities (biocrusts) growing on gypsum soils have been well- documented for their prolific appearance and rich diversity of lichens and bryophytes. However, studies characterizing gypsum biocrusts have primarily occurred outside of the U.S., most of which lack comparisons to other soil types. We conducted intensive field surveys to evaluate the cover and frequency of biocrust functional groups and moss species on gypsum and non-gypsum soils in the U.S. regions with the most extensive gypsum outcrops, the northern Chihuahuan and eastern Mojave Deserts. We employed canonical correspondence analysis (CCA) to relate the observed differences in biocrust abundance and …

Interplay Between Rna Editing And Alternative Splicing Modulates Biophysical And Pharmacological Properties Of The Ion Channel Cav1.3, Willy Munyao

Theses and Dissertations

Alternative splicing and adenosine-to-inosine (A-to-I) RNA editing are post-transcriptional mechanisms that promote proteomic diversity in the brain. The impact of RNA editing on brain physiology is underscored by its association with the etiology and pathogenesis of various neurological and neurodegenerative diseases. Alternative splicing of exon 43 within the CaV1.3 C-terminus produces long (CaV1.3L) and short (CaV1.343S) variants exhibiting distinct gating and pharmacological properties. While the physiological role of CaV1.3 A-to-I RNA editing in brain function is still unfolding, recent findings indicate that unedited CaV1.3 channels display larger calcium influx and enhanced neuronal excitability, suggesting that A-to-I RNA editing may act …

Spatial Variation In Mercury Contamination In Freshwater Fish Species Across Rhode Island, David L. Taylor, Brianna Lotti, Jonathan Serbst, Jim Lake, Alan Libby, Mariel Sorlien

Student Research Symposium

Fish provide essential nutrients and proteins beneficial to human health, yet may pose certain risks due to contaminants in edible tissues. For example, mercury (Hg) is a widespread toxicant that bioaccumulates in fish muscle (filet) tissue. Accordingly, federal and state government agencies issue consumption advisories to minimize diet related Hg exposure. Fish consumption advisories, however, often fail to account for small-scale spatial variations in Hg contamination, especially in freshwater fishes. This study examined site-specific Hg concentrations in bluegill sunfish (Lepomis macrochirus), pumpkinseed sunfish (L. gibbosus), largemouth bass (Micropterus salmoides), chain pickerel (Esox niger), and yellow perch (Perca flavescens), all of …

Maternal Mercury Transfer From Pregnant Spiny (Squalus Acanthias) And Smooth (Mustelus Canis) Dogfish To Their Pups Through Differing Reproductive Strategies, David L. Taylor, Maxwell Ajemian, Abrielle Remick

Student Research Symposium

Mercury (Hg) is a bioaccumulative contaminant that can be transferred from pregnant females to developing embryos (i.e., intrauterine maternal Hg transfer); thus, leading to health deficits in offspring. This study examined Hg transfer in the ovoviviparous spiny dogfish (Squalus acanthias) and viviparous smooth dogfish (Mustelus canis). Female dogfish were collected from Narragansett Bay, Rhode Island Sound, and Block Island Sound via rod & reel, gillnet, and trawling. In the laboratory, intrauterine pups were excised from pregnant dogfish, after which samples of mother and pup muscle tissue were analyzed for total Hg concentrations ([Hg] in ppm dry weight) using automated atomic-absorption …

Mark-Recapture Methods In Eastern Diamondback Rattlesnake (Crotalus Adamanteus) Monitoring, Carissa J. Adams

Theses, Dissertations and Capstones

Eastern diamondback rattlesnakes (Crotalus adamanteus; EDB) are an imperiled species native to the Southeastern United States longleaf pine (Pinus palustris) ecosystem. Several markrecapture methods have been used range-wide in hopes of increasing knowledge on their population numbers. I explored the efficacy of two mark-recapture methods (scale cauterization and dorsal head photography) using the Marine Corps Recruit Depot, Parris Island, SC EDB population in this multi-part study. The first method I evaluated was ventral scale branding, which has been widely implemented without knowledge of brand permanence. I classified misidentification errors at capture status (new versus recapture) and …

Factors Determining The Success Of Recently Planted Spartina Alterniflora On A Living Shoreline In Jamaica Bay, Ny, Abby K. Bressette, Shakira Thomas, Brett F. Branco

Environmental Studies Publications

Natural and nature-based features have been implemented throughout New York City to provide habitat and reduce flooding, erosion, and pollution. The West Pond Living Shoreline in Jamaica Bay, NY was designed with organic breakwaters and native marsh plants, including S. alterniflora. Post-construction monitoring in 2021 found that percent coverage of S. alterniflora was lower than expected. To inform future management, we studied the effect of elevation, planting method, and shoreline energy exposure on the percent cover, average stem height, and average stem density of S. alterniflora. Elevation and plant growth data were collected within 8 one sq. meter quadrats at …

Evaluation Of Hormonal Suppression Treatments Of Endometriosis, Olivia Vermeulen

Biology and Microbiology Graduate Students Plan B Research Projects

Endometriosis is a chronic, multifactorial, and estrogen dependent disease that causes lesions made of endometrial tissue to implant in areas other than the uterus such as the ovaries, fallopian tubes, abdominal cavity, lungs, and even heart (1). These lesions can cause an immense amount of pain in the implanted area during the menstrual cycles and can cause infertility issues, but unfortunately there are not many available and effective treatment options available that do not involve surgically removing the lesions (2). However, there are certain hormonal suppression medications being that are new or are being tested that can alleviate inflammation and …

Unveiling The Past: A Comparative Demographic Analysis Of The Black Population In Columbus, Georgia With Other Contemporaneous Data (1700'S To Present), Torie Hartis, Michael Newbrey, Jennifer Newbrey

CSU Tower Day

No abstract provided.

Two New Species Of Dulcicalothrix (Nostocales, Cyanobacteria) From India And Erection Of Brunnivagina Gen. Nov., With Observations On The Problem Of Using Multiple Ribosomal Operons In Cyanobacterial Taxonomy, Aniket Saraf, Prashant Singh, Naresh Kumar, Sagarika Paul, Jeffrey R. Johansen

2024 Faculty Bibliography

Two new species of Dulcicalothrix, D.adhikaryi sp. nov. and D.iyengarii sp. nov., were discovered in India and are characterized and described in accord- ance with the rules of the International Code of Nomenclature for algae, fungi, and plants (ICN). As a result of phylogenetic analysis, Calothrix elsteri is reas- signed to Brunnivagina gen. nov. During comparison with all Dulcicalothrix for which sequence data were available, we observed that the genus has six ribosomal operons in three orthologous types. Each of the three orthologs could be identified based upon indels occurring in the D1–D1′ helix sequence in the ITS rRNA region …

Four Novel Species Of Kastovskya (Coleofasciculaceae, Cyanobacteriota) From Three Continents With A Taxonomic Revision Of Symplocastrum, Brian Jusko, Jeffrey R. Johansen, Smail Mehda, Elvira Perona, M Angeles Muñoz-Martín

2024 Faculty Bibliography

Studies performed in North America, Africa, and South America have led to the isolation of four new species of Kastovskya, a filamentous cyanobacterial genus that before this manuscript had only one species, Kastovskya adunca from Chile. Kastovskya nitens and K. viridissima were isolated from soils on San Nicolas Island, K. sahariensis was isolated from hypolithic habitats from the Sahara Desert in Algeria, and K. circularithylacoides was isolated from hypolithic habitats in Chile. The molecular analyses are corroborated by morphological data, morphometric analysis, and ecological and biogeographical considerations for robust polyphasic descriptions of all taxa. The peculiar transatlantic distribution of this …

A Multivariate Analysis Of Phytoplankton Assemblages And Environmental Variables In Chautauqua Lake, New York, Emily Palmer

Masters Theses

No abstract provided.

Ms Environmental Biology Capstone Project, Klaudia Sowizral

Regis University Student Publications (comprehensive collection)

Thelephorales fungi are considered important ectomycorrhizal fungi (EMF) in forest ecosystems, yet the patterns of diversity and distribution and the processes that shape them remain poorly understood. This study aims to address this research gap by conducting an exploratory analysis of Thelephorales fungi in soil and litter across various habitat types and environmental characteristics. To do so, we used data collected and synthesized by the Climush project, a multi-year, systematic sampling of macrofungal diversity across North America, that aims to assess sexual (sporocarp and spore trap data) and asexual (soil and endophyte metabarcode data) diversity and distribution in relation to …

Ms Environmental Biology Capstone Project, Sarah Luper

Regis University Student Publications (comprehensive collection)

Recreational trails and the presence of invasive species such as Bromus inermis can both disturb grassland community composition. Trails can act as dispersal corridors for plant material, but can also be a source of trampling and compaction. Invasive grasses like Bromus inermis can easily establish in grasslands and decrease species richness by increasing competition. Understanding these effects, this study aims to understand how both the presence of recreational trails as well as the presence of Bromus inermis affect species richness and overall community composition at Two Ponds National Wildlife Refuge. Belt transect sampling took place in fall 2019 and again …

Patterns Of Genetic Structure In Spiders Beetles, Naiya Sims

Mahurin Honors College Capstone Experience/Thesis Projects

Spider beetles are a poorly known group of North American beetles in the family Ptinidae. Protection of biodiversity requires knowledge of species- and population-level distinctiveness, so data regarding genetic structure of spider beetles can shed light on conservation priorities. Spider beetles in the genus, Coleotestudus, are distributed broadly in the southwestern US. Given their small size and lack of flight, it is presumed that the dispersal capability of Coleotestudus is limited, leading to strong population structure on a small spatial scale. We used a three-enzyme restriction-site-associated DNA sequencing (3RAD) strategy to identify single nucleotide polymorphisms (SNPs) throughout the genome …

Analysis Of Protein-Protein Interactions Between Legionella Pneumophilia Effectors, Briana Harness

Mahurin Honors College Capstone Experience/Thesis Projects

Legionella pneumophila an intracellular, opportunistic pathogen that is the causative agent of Legionnaires’ disease and Pontiac fever. A key component of Legionella’s pathogenicity is a series of effector proteins, which aid in the establishment of the Legionella-containing vacuole (LCV). The abundant library of effector proteins L. pneumophila has within its arsenal gives L. pneumophila the ability to infect several different hosts. The pathogenesis of L. pneumophila within various hosts depends on its ability to modulate the activity of its effector proteins through protein-protein interactions within effectors and eukaryotic host cell proteins. By understanding how L. pneumophila regulates its effectors, a …

Mercury Content In The Baleen Plates Of Rorqual Whales, David L. Taylor, Ava Cieplinkski, Max Ajemian, Matthew Savoca

Student Research Symposium

Mercury is a persistent environmental contaminant posing significant risks to wildlife health due to its propensity for bioaccumulation in organismal tissues. This study investigated total mercury concentrations in the baleen plates of rorqual whales (Family Balaenopteridae), specifically the Fin whale (Balaenoptera physalus), Blue whale (B. musculus), and Rice’s whale (B. ricei). Archived baleen samples were sourced from the Smithsonian’s National Museum of Natural History and analyzed for total mercury (Hg) content (ppm dry weight) using atomic absorption spectroscopy. Male Fin and Blue whales from the Antarctic, South Pacific region (1947-1948), with body lengths ranging from 21 to 24 m, exhibited …

Environmental Biology Masters Capstone, Antonio Gonzalez-Pita

Regis University Student Publications (comprehensive collection)

Human wildlife interactions (HWI) pose a complex challenge for wildlife managers. Human encroachment into wildlife habitat and the growing number of outdoor recreationists are increasing the frequency of contact and conflict, especially in regions such as the Front Range of Colorado. Geographic information systems (GIS), which use a combination of remote sensing and environmental survey data, allow for predictive spatial analyses of where human wildlife interactions are likely to occur. I used publicly reported observations of moose to create spatial predictive maps in a species distribution model framework. Slope and elevation were shown to be the strongest predictors of HWI, …

The Influence Of Abiotic Stress And Cultivar Variation On Plant Defenses And Resistance To Emerald Ash Borer (Agrilus Planipennis) In Cultivated Olive (Olea Spp), Idris B. Elesin

Browse all Theses and Dissertations

Emerald ash borer (EAB), Agrilus planipennis, is an invasive pest that significantly impacts olive trees (Olea europaea). This study aimed to assess the role of abiotic stress factors such as drought and salinity in influencing plant defenses and resistance to EAB, while also examining cultivar variation in resistance. The study specifically aimed to address: (1) What effects do varying degrees of abiotic stress (drought, salinity) have on olive trees' antioxidant and peroxidase activity in olive tree? (2) Do distinct olive cultivars exhibit varying levels of resistance to EAB under various stressors? We used a controlled experimental design with two stress …

Role Of Insulin-Like Growth Factor-1 Signaling In Diet-Enhanced Bone Elongation, Cassaundra A. White

Theses, Dissertations and Capstones

Childhood obesity has increased precipitously to the point that almost 20% of children between the ages of 2 and 19 have been diagnosed with obesity. While obesity is a complex metabolic disorder, one contributing factor is diet, particularly a diet high in fat. Among many problems associated with diet-induced obesity, the impact on skeletal health is often overlooked. In addition to increased stress on the skeleton due to excess weight, obesity also disrupts the endocrine and immune systems that, in turn, can secondarily alter skeletal growth. Obesity during the early years of childhood is particularly detrimental because the skeleton is …

Locus-Specific Differential Expression Of Human Satellite Sequences In The Nuclei Of Cancer Cells And Heat-Shocked Cells, Christina Rabeler, N. Paterna, Rajiv Potluri , '20, Lia R. D’Alessandro , '21, Anusha Bhatia , '24, Shu Yi Chen , '24, Johanna Lee , '23, Bereketab Abeje , '23, Benjamin Lipchin , '25, B. R. Carone, Dawn M. Carone

Biology Faculty Works

Human satellites (HSats) are pericentric, tandemly repeating satellite DNA sequences in the human genome. While silent in normal cells, a subset of HSat2 noncoding RNA is expressed and accumulates in the nucleus of cancer cells. We developed a FISH-based approach for identification of the distribution of three subfamilies of HSat2 (A1, A2, B) sequences on individual human chromosomes. Further, using the HSat subfamily annotations in the T2T completed centromere satellite (CenSat) sequence, we isolated, defined and mapped differentially expressed sequence variants of nuclear-restricted HSat2 and HSat3 RNA from cancer cell lines and heat-shocked cells. We identified chromosome-specific and subfamily-specific expression …

The Iglon Family Of Cell Adhesion Molecules Expressed In Developing Neural Circuits Ensure The Proper Functioning Of The Sensory System In Mice, K. Singh, M. Jayaram, A. Hanumantharaju, T. Tõnissoo, T. Jagomäe, K. Mikheim, S. Muthuraman, Scott F. Gilbert, M. Plaas, M. K. E. Schäfer, J. Innos, K. Lilleväli, M.-A. Philips, E. Vasar

Biology Faculty Works

Deletions and malfunctions of the IgLON family of cell adhesion molecules are associated with anatomical, behavioral, and metabolic manifestations of neuropsychiatric disorders. We have previously shown that IgLON genes are expressed in sensory nuclei/pathways and that IgLON proteins modulate sensory processing. Here, we examined the expression of IgLON alternative promoter-specific isoforms during embryonic development and studied the sensory consequences of the anatomical changes when one of the IgLON genes, Negr1, is knocked out. At the embryonal age of E12.5 and E13.5, various IgLONs were distributed differentially and dynamically in the developing sensory areas within the central and peripheral nervous …

Noise And Light Pollution Elicit Endocrine Responses In Urban But Not Forest Frogs, J. A. H. Smit, R. Vooijs, P. Lindenburg, Alexander T. Baugh, W. Halfwerk

Biology Faculty Works

Urban areas are characterised by the presence of sensory pollutants, such as anthropogenic noise and artificial light at night (ALAN). Animals can quickly adapt to novel environmental conditions by adjusting their behaviour, which is proximately regulated by endocrine systems. While endocrine responses to sensory pollution have been widely reported, this has not often been linked to changes in behaviour, hampering the understanding of adaptiveness of endocrine responses. Our aim was, therefore, to investigate the effects of urbanisation, specifically urban noise and light pollution, on hormone levels in male urban and forest túngara frogs (Engystomops pustulosus), a species with …