Biology Commons^™

Multivariate Ecological Assessment Of Flora Of Village Karri Haider, District Tank, Kp, Numan Khan, Muhammad Azeem, Muhammad Adnan Hamza, Muhammad Aleem, Arshad Rasool, Ammara Wakeel, Sehrish Sadia

Journal of Bioresource Management

Spatial and temporal patterns of vegetation change are influenced by multiple factors, including the physical environment, land-use history, prior disturbance, and initial vegetation composition. The primary aim of this study was to understand floristic vegetation features, species diversity, and the classification of plant communities in Village Karri Haider, District Tank, Khyber Pakhtunkhwa (KP). Samples were collected from each transect using quadrats of 1 m × 1 m for herbs. Soil from three quadrats was mixed to make composite samples, and soil elements were quantified through laboratory analyses. Two surveys were conducted from July to December, 2023. In the first survey, …

From Genes To Streams: Mitochondrial Approaches To Population Monitoring Of The Santa Ana Sucker Catostomus Santaanae, Mariana Antonio

Electronic Theses, Projects, and Dissertations

This study was developed to use aquatic environmental DNA (eDNA) as a tool to detect the presence of the Santa Ana sucker (Catostomus santaanae), a federally threatened fish species, across the Santa Ana River in Southern California. Environmental DNA is any genetic material that an organism sheds into the environment. We can get an idea of what aquatic species are present when sampling water at a given site. We sequenced the complete mitochondrial genome (mitogenome) of the Santa Ana sucker and designed custom DNA primers to improve eDNA detection accuracy. These primers were selected based on high genetic …

Using Temporal Genomic Data To Uncover Patterns Of Genetic Diversity And Signatures Of Selection For Management Of A Threatened Minnow Species, Erika A. Dunn

Biology ETDs

The Little Colorado River (LCR) spinedace persists only in three isolated watersheds within the LCR Basin in Arizona. This imperiled minnow faces ongoing pressure from habitat alteration, climatic shifts, and invasive non-native predators. Over the past two decades, managers have translocated spinedace within watersheds to increase abundance and expand habitat occupancy. We sequenced a reference genome and analyzed single nucleotide polymorphisms (SNPs) from 475 individuals over their geographic range. DNA samples were obtained from a previous study in the mid-1990s and compared with samples from 2024 and 2025, allowing us to track genetic diversity, effective population size, population structure, and …

Scientific Note: Here The Whole Time: A Revised Understanding Of White Alumroot (Heuchera Alba, Saxifragaceae) In The Susquehanna River Valley., Anais Barnes, Madeline Wickers, Scott Schuette, Tanisha M. Williams, Ryan Folk, Christopher T. Martine

Faculty Journal Articles

Heuchera alba and H. pubescens (Saxifragaceae) are closely related species of alumroot endemic to the Appalachian region of eastern North America that can be difficult to distinguish morphologically. Heuchera pubescens has been understood to occupy a range from Kentucky to Pennsylvania, with the distribution of H. alba restricted to Virginia and West Virginia—plus a recently-recorded extension into Pennsylvania discovered with the help of social media. The uncovering of H. alba in Pennsylvania has challenged historical perceptions of Heuchera distributions in the state, particularly as this relates to the current concept of H. pubescens which may be in need of revision. …

Life Histories Of Two Sgcn Procambarus Species In East Texas, Mckenzie A. Adkins

Biology Theses

Within freshwater systems, crayfish play notable roles by shaping habitats and linking food web levels. Yet even with such influence, knowledge about numerous species is incomplete, which hinders protection strategies. Found only in the Neches River basin in eastern Texas, Procambarus nechesae and Procambarus nigrocinctus have different conservation statuses, mainly because data on their life history remains sparse. These two species are not closely related and are analyzed independently in this study. Research into traits tied to survival and environmental preferences was carried out so that informed decisions on conservation efforts can be made for these species. The goal of …

Assessing The Conservation Needs Of Native Texas Crayfish Species Through Distribution Analysis And Species Distribution Modeling, Elizabeth J. Croft

Biology Theses

Crayfish have essential roles in ecosystems because they are ecosystem engineers and keystone species. However, crayfish species are threatened by degradation and loss of habitats, introduction of invasive species, and high levels of endemism. Texas provides no legal protections for crayfish, despite its large number of species and endemics in the state. The overall goal of this study is to clarify the distributions of native Texas crayfish species using literature records, citizens science, and field sampling data, and highlight species in need of more conservation research. Occurrence records from all collected data were combined to visualize the distributions of species …

Investigating The Taxonomic Uncertainties Of Potentilla Rupincola, Natalie Elizabeth Hieber

Master's Theses

Landscape stewardship is an integral role for land managers that becomes more important when rare or endangered species occur within managed areas. Conservation across the landscape is a primary concern for land managers, but resources allocated for conservation can be limited due to many competing goals. Taxonomic uncertainty surrounding rare and endangered taxa, particularly plant taxa, that have historically been classified solely using morphological features further complicates conservation planning. One such taxon is Potentilla rupincola, a rare plant endemic to the eastern Rocky Mountains in Colorado. For over a century, botanists have debated whether P. rupincola is a distinct species …

Characterization Of A Core Fungal Community And Captivity-Induced Gut “Mycobiome” Change In Fowler's Toad (Anaxyrus Fowleri), Alexander J. Bradshaw, Sinlan Poo, Tracey E. Malter, Rylie M. Strasbaugh, Brianna Bodner, Madison R. Hincher, Anne Devan-Song, Javier F. Tabima

Biology

Amphibious animals, such as frogs, are found at the intersection of aquatic and terrestrial ecosystems. They may serve as keystone and sentinel species and play key roles in nutrient cycling and food webs. In recent decades, amphibians have experienced drastic population declines due to habitat loss, climate change, and disease. These declines have prompted investments in ex situ conservation and captive breeding programs, which aim to reduce extinction risk by creating assurance colonies and reintroducing individuals once threats are mitigated. A critical component of these programs is proper husbandry, which ensures the health and longevity of captive populations and their …

Cryptic Diversity Within Nyctixalus (Anura: Rhacophoridae): A New Species, A Resurrection, And A Redescription, Jennifer Dever, Jimmy A. Mcguire, Djoko T. Iskandar, Amir Hamidy, Lee L. Grismer

Biology Faculty Publications

The examination of molecular markers confirms hidden diversity and reveals a new species within the Nyctixalus pictus (Peters, 1871) complex. Three genetically distinct clades were recovered: one from the Thai-Malay Peninsula; one from Sumatra, Indonesia; and one from Sarawak, Borneo, East Malaysia. Voucher specimens from each region were compared, and significantly different mean values were found through morphological analyses. The populations also differ significantly in morphospace, as determined by PCA, DAPC, and MFA analyses. The Thai-Malay Peninsula individuals have a distinct, rounded and slightly elongated snout shape compared to the other two clades. Based on these combined data, these three …

Assessing Urban–Rural Differences In Largemouth Bass Abundance In Minnesota, Jonathan Gates

Journal of Earth and Life Science

Urbanization can significantly alter freshwater ecosystems and influence sport fish populations. This study examined differences in largemouth bass Micropterus nigricans catch-per-unit-effort (CPUE) between urban and rural lakes in Minnesota and evaluated temporal changes between 2000–2010 and 2011–2021. Forty lakes (20 urban, 20 rural) were selected using Minnesota Department of Natural Resources fisheries survey data. Lakes were classified using NLCD 2019 land cover data, with ≥10% developed land designated as urban. Nonparametric analyses (Wilcoxon rank-sum and signed-rank tests) were used to assess spatial and temporal differences in CPUE. Results showed that rural lakes had consistently higher median CPUE than urban lakes …

Analyzing The Impact Of Diet On Dusky Gopher Frog (Lithobates Sevosus) Development In Head Start Programs, Mckalyn Nelson

Honors Theses

Species are constantly impacted by human activity, directly or indirectly. One critically endangered species under investigation is the dusky gopher frog (Lithobates sevosus), which is an endemic species of frog found in the southeastern United States. Historically found from Louisiana to Alabama, a drought in 2002 narrowed the dusky gopher frog range even further. Head start programs have been shown to be effective in increasing population numbers; however, there is not much published data on the effectiveness of captive rearing diets for these programs. The dusky gopher frog head start program at the University of Southern Mississippi’s Lake …

Improving Coral Restoration Outcomes Through Novel Molecular Applications, Matthew H. Gamache

USF Tampa Graduate Theses and Dissertations

Coral reef ecosystems are declining globally from numerous stressors, including localized pollution, disease, and marine heatwaves induced by climate change. To preserve these ecosystems, it is critical to identify corals that can withstand stressors—primarily future marine heatwaves—and prioritize them for coral restoration efforts until these stressors are mitigated. Coral responses to thermal stress can be influenced by several partners, including mutualistic microalgae and a vast prokaryotic community. Here, I use advanced molecular techniques to examine which partners (coral host, algal symbiont, and microbial communities) are associated with coral health, how they contribute to holobiont physiology, and their potential to increase …

Biodiversity Conservation Depends On The Expansion Of Taxonomy And Systematics Research, Luis A. Ruedas, Scott L. Gardner

Biology Faculty Publications and Presentations

Despite mammals constituting fewer than 0.3% of all described species, their conservation is compelling for a number of reasons. They contribute to biodiversity and are important in maintaining and regulating ecological communities. Many mammals have significant cultural and economic value, serving as sources of food, medicine, and tourism revenue. Furthermore, the conservation of mammals often leads to the protection of entire habitats, benefiting numerous other species and preserving ecosystem services that are critical for human well- being. Humans share a unique evolutionary history with other mammal species, making their preservation important for scientific research, education, and understanding of our own …

Using Vegetation Communities To Compare Natural And Constructed Wetlands In The Red River Basin In Texas And Louisiana, Kennedi K. Davis

Biology Theses

Wetland vegetation provides an abundance of ecosystem services such as water quality regulation, flood control, and it provides habitat space for fish, amphibians, and macroinvertebrates. Not only that, but these plants also act as excellent indicators of wetland conditions as dominant vegetation types are often used as biological criteria for classifying wetlands and other habitats (Cowardin and Golet, 1995). This project will assess the structure of vegetation communities to determine how they differ in constructed and natural wetlands within the Red River drainage basin to see if Agricultural Conservation Easement Program – Wetland Reserve Easement (ACEP-WRE) constructed wetlands are effective …

Restoration At The Intersection Of Science And Outreach-The Working Woods Learning Forest, Katharine L. Stuble, David J. Burke, Samuel C. Harbol, Chad Knisely, Jessica Miller Mecaskey, Alexa S. Wagner, Michael Watson, Kevin E. Mueller

Biological, Geological, and Environmental Faculty Publications

Botanical gardens and arboreta have enormous (if often under-realized) potential to increase understanding of restoration as a predictive science while simultaneously serving as platforms promoting large-scale restoration by public and private stakeholders. Public gardens often have deep histories of both research and outreach and, increasingly, engagement in restoration efforts. As a team of researchers and practitioners, we share our experiences using a forest management manipulation at the Holden Arboretum for both research and outreach. Forest management is a key tool for enhancing biodiversity and ecosystem services in young forests. Known as the Working Woods Learning Forest, this restoration simultaneously serves …

Using Herpetofauna To Determine Wetland Health And Function In The Red River Watershed, Shelby Rodriguez-Edwards

Biology Theses

Reptiles and amphibians are commonly used indicator species in wetlands because of their increased sensitivity to environmental conditions and use of both aquatic and terrestrial habitats. Herpetofauna can reflect wetland ecological functionality through their community composition and responsiveness to environmental stressors. This study evaluated reptile and amphibian communities in natural and ACEP-WRE wetlands within the Red River Watershed of northeastern Texas and Northwestern Louisiana to assess whether restored wetlands support herpetofaunal assemblages compares to their natural counterparts. Fifteen wetlands were surveyed from February to June 2024 using visual encounter surveys, call surveys, and standardized trapping methods to document species richness …

Scientific Note: Garden Loosestrife (Lysimachia Vulgaris, Primulaceae), An Emerging Invasive Plant Species Of Riparian Corridors In The Mid-Atlantic Region, Christopher T. Martine, Amy L. Jewitt, Claire M. Ciafre, Kaitlin G. Henry, Elizabeth H. Williams, Pj Newhart, Melody P. Sain

Faculty Journal Articles

Lysimachia vulgaris (garden loosestrife, Primulaceae) is an herbaceous perennial native to Europe that has been identified as an aggressive invasive species in parts of North America. The status of the species in the Mid-Atlantic region of the US, however, is poorly determined. Using recent field observations (from iNaturalist, iMapInvasives, and EDDMapS records) we here report localized incursions of L. vulgaris in the Susquehanna River Valley of central Pennsylvania, along with notes on its ecology, threats to native plant communities, and broader distribution in the state.

Field Journal: The Excluded Middle, Carrie E. Kouts

Masters Theses

What does it look like to engage with organisms and landscapes at the periphery of anthropocentric value structures? How does one break the internalized myth that the “built” environment is excluded from the natural world? When does a hyper-mobile and hyper-commodified society confront the exponential crisis of animal death? Within this series of journal entries, field notes, collection observations, weird prose, and sensory musings, one will find questions on the nature of being human and the complex narratives of care we encounter in a world shared with more-than-humans. Each handwritten vignette and photograph from daily life weaves a non-linear and …

Comparative Methylation Analyses Across Juglans Species To Investigate Epigenetic Contributions To Fungal Resistance, Keertana Chagari

Honors Scholar Theses

Juglans cinerea (butternut) is a critically threatened North American tree species experiencing severe declines due to the fungal pathogen Ophiognomonia clavigignentijuglandacearum. In contrast, its Asian relative, Juglans ailantifolia (Japanese walnut), shows natural resistance. To investigate the genomic and epigenetic factors underlying this difference, we constructed and analyzed high-quality, chromosome-level genome assemblies for both species from long-read sequencing data (J. ailantifolia: 527Mb; J. cinerea: 586Mb), focusing on transposable element (TE) content, DNA methylation patterns, and regulation of pathogen resistance genes (PRGs).

Repeat analysis revealed that J. cinerea has a slightly higher overall transposable element (TE) content, with …

A Floristic Study Of The Vascular Flora Of The Brokeoff Mountains, Southcentral New Mexico, Bryana L. Olmeda, Harpo Faust, Hannah E. Marx

Biology ETDs

The vascular flora detailed here describes 438 km² (169 mi²) of the Brokeoff Mountains of southeastern New Mexico. The Brokeoffs are part of the northern extent of the Chihuahuan Desert, the largest desert in North America and one of the ecoregions least represented in botanical collections. Prior to 2023, only 254 plant collections were known from the study site, representing roughly 1.5 collections per 2.59 km² (1 mi²), despite strong research interest in its neighboring Guadalupe Mountains. Over two field seasons, 1920 plant collections were made, over 81 days of field work conducted, in …

Balancing Burrowing Owl (Athene Cunicularia Hypugaea) Protections While Maintaining Renewable Energy Development Within The San Joaquin Valley, Madison Lenzi Mcginn

Master's Projects and Capstones

Balancing the needs of wildlife and people has long been a complex challenge. The San Joaquin Valley exemplifies this tension, as space for agriculture and urbanization compete for land use of native grasslands– often resulting in the conversion of grasslands for agriculture fields, commercialized areas, or energy development. With an increasing number of agricultural landscapes going fallow and the passing of SB-100 in 2018, solar farm development has emerged as an attractive option to offset financial losses from unproductive farmland. However, the same landscapes favored for solar development also provide suitable habitat for sensitive species such the western burrowing owl …

Genomic Structure Among Populations Of A Regionally Rare Perennial Plant Indicates The Need For Reevaluation Of Management Units, Cheyenne Moore, Angela J. Mcdonnell, Scott Schuette, Christopher T. Martine

Faculty Journal Articles

Conservation of rare species often relies on the delineation of management units and genomic tools can now be applied for this purpose. However, this is not as common on a local scale, where populations are often small and fragmented, despite the utility of informing conservation and management practice at the regional level. We use a genotyping by sequencing (GBS) approach to assess the perennial riparian plant species Baptisia australis (Fabaceae) in Pennsylvania, where the taxon is at the edge of its natural distribution and considered threatened. In this system, we investigate whether sampled subpopulations exhibit genetic structure. We find that …

Assessing Phenotypic Variation Across Generations Of Iberodes Littoralis Under Climate Change, Mariangel M. Fernandez Silva

Electronic Theses, Projects, and Dissertations

The consequences of climate change and anthropogenic activity have decreased wildlife biodiversity. Plants are particularly vulnerable to climate change due to their large biomass on the planet and sessile nature. A particularly useful method for plants to cope with environmental changes is phenotypic plasticity. Adjusting morphological traits through plasticity can help plants cope with environmental changes. Furthermore, plastic responses can be passed down through methods such as changes in DNA sequence or epigenetic modifications.

Our research project focuses on an herb, Iberodes littoralis, a species of concern native to the Siberian Peninsula with approximately thirteen extant populations. The data that …

All Stressed Out: An Investigation On The Effects Of Environmental Stressors On The Northern Leopard Frog (Rana Pipiens), Ella C. Wittmuss

Honors Thesis

Corticosterone (CORT) is the primary glucocorticoid in many vertebrates, including mammals, birds, reptiles, and amphibians. When an organism is exposed to external or internal stressors, CORT levels often increase (Rich & Romero, 2005; Titon et al., 2021). An increase in CORT can cause adverse physiological effects such as slower growth, malformations in different parts of the body, and specific blood sugar diseases such as diabetes (Glennemeier & Denver, 2002; Tarlow &Blumstein, 2007). CORT levels are indicators of survival, growth, and reproduction in larval amphibians. Many species, including our model organism, the Northern Leopard Frog (Rana pipiens), are often subjected to …

Demography And Habitat Associations Of The Basalt Cactus Pediocactus Nigrispinus, Zarha S. Dillon-Zuppelli, Mary E. Poulson, Eric A. Graham

Biology Faculty Scholarship

Cacti are iconic members of arid ecosystems. Pediocactus nigrispinus is a species of cactus endemic to the Pacific Northwest and has been listed as a sensitive species due to its vulnerability to habitat loss. Best conservation methods for this species are poorly understood, due in part to a limited understanding of its demography and habitat associations that may help define its occurrence. A demographic study from four plots in Washington State was implemented in 2016 to provide a baseline of the population dynamics for P. nigrispinus and to enhance conservation efforts for this sensitive species. Five years of size, fecundity, …

Measuring Spatial And Temporal Variation In Species Composition Of Ephemeral Pond Amphibian Assemblages Using Metabarcoding, Elizabeth Strasko

Masters Theses & Specialist Projects

Amphibian populations worldwide face severe threats, heightening the need for reliable monitoring methods. In this thesis, I explored and tested environmental DNA (eDNA) metabarcoding protocols to survey amphibian assemblages in ephemeral ponds of the the Green River Preserve in central Kentucky, USA. First, I evaluated whether the commonly used mitochondrial cytochrome c oxidase subunit I (COI) gene could effectively capture species-level variation in local amphibians for eDNA metabarcoding. Extensive primer design and testing revealed significant challenges in amplifying fragmented eDNA from water samples. In a second study, I applied a metabarcoding approach using the mitochondrial 12S ribosomal RNA (12S) gene …

Understanding The Individual Behavioral Variation Of Asian Elephants (Elephas Maximus) As An Indicator Of Human Elephant Conflict, Julia Brzezicki

Masters Theses & Specialist Projects

Human-Elephant Conflict (HEC) is an increasingly common event that arises from resource competition due to overlaps in human and elephant environments. Using a conservation behavior approach, the behaviors of elephants can be studied to enhance conservation efforts to mitigate HEC by better predicting which elephants engage in HEC. To understand the factors that affect individual behavioral types of adult Asian elephants (Elephas maximus), my project examined the behavioral patterns displayed by an elephant population in Sri Lanka. I collected demographic and behavioral data of a subset of 15 adult elephants to determine individual differences in five behavioral categories: Affiliation, aggression, …

The Influence Of Anthropogenic Infrastructure On The Reproductive Success Of Northern House Wrens (Troglodytes Aedon) In Proximity To Highways, Nana-Esi Irie Hanson

Master's Theses or Doctor of Nursing Practice

Anthropogenic disturbances, particularly road infrastructure and noise pollution, significantly affect avian populations, influencing their ecology, behaviour, and reproductive success. This study examined the impact of highway proximity on the reproductive success of the Northern House Wrens (Troglodytes aedon) in a rural landscape. Noise levels and reproductive outcomes were compared between two sites in Hays, Kansas: a high-traffic site near Interstate 70 (Sternberg Museum Nature Trail) and a lower-traffic site at Big Creek on the Fort Hays State University campus. I hypothesized that increased traffic volume and noise pollution would negatively affect Northern House Wren reproductive success. Ambient noise …

Determinants Of Bat Activity And Habitat-Use At Runkle Woods And Huffman Metropark, Ohio, Heidi E. Orloff

Browse all Theses and Dissertations

North American bats are an excellent control of pests as most are insectivorous feeders. They face numerous threats including habitat loss, anthropogenic development, disease (White-nose Syndrome), and potentially artificial light at night (ALAN). It is important to track species populations and habitat-use to determine the magnitude of effects from these threats. Acoustic monitoring has proven a useful and non-invasive method for tracking bat populations. In my research, I conducted stationary acoustic surveys of bat activity at Wright State University’s Runkle Woods and nearby Huffman MetroPark during the summers of 2023 and 2024. I recorded bat calls in three different habitats …

Rooting For Resilience: Investigating The Success Of Seagrass Restoration Methods, Olivia K. Stockdale

The Plymouth Student Scientist

Seagrasses generate vital ecosystem services to coastal communities, whilst also protecting marine biodiversity. Despite their significance, seagrass ecosystems face a plethora of threats from both anthropogenic and natural sources, causing a 20 % loss of seagrass area over the last century. To combat this, numerous restoration methods exist for seagrass habitats globally, with varying degrees of success. Through a comprehensive systematic review and subsequent meta-analysis, this study illustrates the varying success rates of different transplant and seed-based restoration methods over differing timescales. Accounting for varying timescales, results showed that restoration method had a significant effect on success rate (χ^{2 …}