Biology Commons^™

Temperature And Salinity Effects On The Physiology Of Bullfrog Tadpoles (Lithobates Catesbeianus) From A Suburban Temperate Wetland, Angela Brown

Theses, Dissertations and Culminating Projects

One of the most robust amphibians is the American Bullfrog, Lithobates catesbeianus, which is a widespread species that is invasive worldwide, and is broadly tolerant of a range of environmental conditions. Despite this tolerance, survival and performance of adult L. catesbeianus can be negatively affected by increasing temperatures and salinity resulting from anthropogenic impacts, such as climate change and road salts. This study focuses on the effects of acute salinity exposure (mimicking road salt influx into a pond) on L. catesbeianus tadpoles at different larval stages. Increased salinity was predicted to negatively affect two physiological performance measures. Critical maximum temperature …

Validating The Expression Of Genes Potentially Involved In The Development Of Aggressive Melanoma Tumor In The Zebrafish Melanoma Model, Mayra Matos Diaz

Theses, Dissertations and Culminating Projects

Melanoma is an aggressive form of skin cancer characterized by rapid progression, metastatic potential, and the frequent development of resistance to targeted therapies. Although the Inducible cAMP Early Repressor (ICER) has been identified as a potential tumor suppressor in melanoma, the molecular mechanisms underlying its role in tumor progression remain poorly understood. Building upon the transcriptomic findings of Wheelan et al. (2025), this study investigates the relationship between ICER and ten candidate genes (gdf6a, pdgfra, col4a4, fgfr2, pdgfrb, cdkn1a, fgf5, akt1, met, and notch3) associated with BMP signaling. Quantitative polymerase chain reaction (qPCR) was used to compare gene expression between …

The Evolution Of Reproduction Without Blood Feeding In An Invasive Vector Mosquito Aedes Albopictus, Samantha L. Sturiale, Mara Heilig, Matthew L. Aardema, Luciano V. Cosme, Margaret Corley, Sarah Marzec, Micah Hamilton, Daniel Vizcarra, Luke T. Anderson, Christina M. Holzapfel, William E. Bradshaw, Megan E. Meuti, Adalgisa Caccone, Peter A. Armbruster

Department of Biology Faculty Scholarship and Creative Works

Background: Mosquitoes transmit a diverse range of deadly pathogens, including dengue and Plasmodium spp., when they consume the blood of vertebrate hosts in order to nutritionally provision their eggs. However, mosquito species from multiple genera have independently evolved the ability to produce eggs without blood. The molecular and physiological processes underlying the evolution of blood-independent reproduction are largely unresolved, in part because the two most well-studied models of mosquito reproductive physiology and genetics are obligate blood feeders. Herein, we integrate life-history, genomics, and gene expression to identify physiological processes and candidate genes associated with the evolution of blood-independent reproduction in …

Decomposition In Urban Forest Patches Across A New York-New Jersey Urbanization Gradient, Cailee Sprouse

Theses, Dissertations and Culminating Projects

"Urbanization causes habitat fragmentation, pollution, homogenization of soil profiles, and replacement of native flora and fauna, which can alter vital ecological processes like decomposition. It is essential to investigate the impacts urbanization may have on ecosystem functioning to better inform urban land management. Studies have shown conflicting results on the impacts of urbanization on soil decomposition rates, with some showing higher, lower, or similar decomposition rates in urban soils compared to rural soils. This study aims to investigate the effects of urbanization and soil characteristics on decomposition rates in three urban forest patches in New Jersey and New York using …

Individual Recognition: Do Horned Passalus, Odontotaenius Disjunctus, Display Individual Discrimination Of Familiar And Unfamiliar Conspecifics Through Changes In Their Behavior?, Sabrina Galloza

Theses, Dissertations and Culminating Projects

The horned passalus, Odontotaenius disjunctus, are subsocial beetles that inhabit and defend decaying wood where they create galleries to live, mate, breed and provide brood care. The species is well known for their stridulations, making up to 14 different sounds, having one of the greatest repertoire of sounds for any known arthropod. Typically living in family units, it has been found that several families can inhabit the same log, therefore, the horned passalus can come into contact with both kin and non-kin. It is currently unknown whether the horned passalus can distinguish between nestmates and non-nestmates. Additionally, it is unknown …

Differential Salinity Tolerance And Compositional Shift In Zooplankton Communities From Coastal And Inland Wetlands, Marisa Contreras

Theses, Dissertations and Culminating Projects

Anthropogenic salinization from road salt application has degraded freshwater ecosystems across the United States, yet the tolerance of freshwater communities to elevated salinity may depend on their historical exposure. Coastal systems experience natural salt intrusion through tides and storm events, potentially fostering greater salinity tolerance amongst resident organisms, while inland systems lack this exposure history. To investigate, I studied the abiotic and biotic responses of zooplankton communities from four wetlands across a salinity gradient to assess tolerance to experimental salt exposure. Two inland freshwater wetlands are subject to anthropogenic salinization from road salt runoff, while two coastal wetlands experience natural …

Shape Variation In The Mandible Of The Borophagine Canids And Implications For Predatory Behavior, Benjamin Neavear

Theses, Dissertations and Culminating Projects

The borophagines are an extinct lineage of canids that inhabited North America for more than 20 million years. This group included genera such as Aelurodon, Borophagus, and Epicyon. Borophagines are commonly referred to as “bone-crushing dogs” because their skull morphology converged with that of extant hyenas, predators capable of consuming bone. The purpose of this study was to determine whether convergence was present in the mandible, an anatomical structure strongly correlated with feeding behavior, particularly in the genera Aelurodon and Borophagus. It was hypothesized that most borophagine taxa possessed mandibular shapes similar to those of hyenas. Numerous mandibular characters were …

Cis-Regulatory Evolution Of Wnt Family Genes Contributes To A Morphological Difference Between Silkworm Species, Kenta Tomihara, Ana Pinharanda, Young Mi Kwon, Andrew M. Taverner, Laura S. Kors, Matthew L. Aardema, Julia C. Holder, Lin Poyraz, Takashi Kiuchi, Peter Andolfatto

Department of Biology Faculty Scholarship and Creative Works

Closely related species often exhibit distinct morphologies that can contribute to species-specific adaptations and reproductive isolation. One example is Lepidopteran caterpillar appendages, such as the “caudal horn” of Bombycoidea moths, which have evolved substantial morphological diversity among species in this group. Using interspecific crosses, we identify the genetic basis of the caudal horn size difference between Bombyx mori and its closest relative Bombyx mandarina. The three largest of eight QTL account for one third the mean horn length difference between the species. The largest of these, on chromosome 4, encompasses a conserved Wnt family gene cluster, key upstream regulators …

Science Communication Tools: Rubrics For Generating Posters And Manuscripts That Are Authentic To The Practice Of Science, Quinn Vega

Department of Biology Faculty Scholarship and Creative Works

Traditional undergraduate science courses often prioritize content mastery over authentic engagement with the scientific process. Course-based research, also referred to as course-based undergraduate research experiences (CUREs), addresses this limitation by immersing students in authentic scientific practice. In course-based research, assessment practices can also mirror the authentic scientific practice, where extensive formative feedback supports refinement of skills and understanding. Here, we present two rubrics designed to support the teaching and assessment of science communication in a way that reflects how scientists prepare to disseminate their research findings. One rubric is for creating scientific posters and another for writing short-format manuscripts. Developed …

American Kestrel Population Trends And Vital Rates At The Continental Scale, Paige E. Howell, Abigail J. Lawson, Kristin P. Davis, Guthrie S. Zimmerman, Orin J. Robinson, Matthew A. Boggie, Mitchell J. Eaton, Fitsum Abadi, Jessi L. Brown, Julie A. Heath, John A. Smallwood, Karen Steenhof, Ted Swem, Brian W. Rolek, Christopher J.W. Mcclure, Jean Francois Therrien, Karl E. Miller, Brian A. Millsap

Department of Biology Faculty Scholarship and Creative Works

The American kestrel (Falco sparverius, hereafter referred to as kestrel) has declined across much of its North American range since at least the mid-1960s. Kestrel population dynamics have been explored through a multitude of local studies and two broad reviews of available data. Across large geographic extents, however, the demographic cause(s) of kestrel population declines remain(s) largely unknown. As part of a collaborative effort to elucidate the drivers of kestrel population declines, we developed a continental-scale integrated population model using band-recovery data, productivity data, and Breeding Bird Survey indices from 1986 to 2019 to estimate indices of annual population sizes, …

Downstream Persistence Of Cyanobacteria In New Jersey's Raritan River Basin, Kyle R. Clonan, Meiyin Wu, Heather Heckathorn, Heather A. Desko, Eric Ernst, Pam Reilly, Bob Schuster, Robert Newby, Annie C. Hurley, Alessandra Rossi

Department of Biology Faculty Scholarship and Creative Works

Few studies have examined cyanobacteria persistence starting from lacustrine cyanobacteria harmful algal blooms (HABs) downstream. Multiple lakes and reservoirs within New Jersey's Raritan Basin Water Supply Complex (RBWSC) feature recurrent HABs and discharge water into the Raritan River. As the RBWSC provides drinking water to 1.5 million people, these HABs create drinking water treatment and human health concerns. This study quantitatively reviews the persistence of cyanobacteria communities across the RBWSC from cyanobacteria-dominated lacustrine sources to downstream drinking water and qualitatively assess what, if any, water chemistry parameters influenced the persistence, growth, or decline of cyanobacteria during downstream transport. Statistical tests …

Crispr Transgenic Clytia Hemisphaerica As A Vector For Toxin Delivery In A Zebrafish Melanoma Model, Theodore G. Paz

Theses, Dissertations and Culminating Projects

The jellyfish Clytia hemisphaerica has the potential to be used in cancer therapies with the discovery of potential active toxin target regions to be modified for use. The program Venomix is a Uniprot-based database that was utilized in this experiment to generate likely flagged hit regions using Clytia hemisphaerica CDS files run against its database of terrestrial toxins (Furuno et al., 2003; Macrander et al., 2018). By themselves they are indeterminate as to whether they are active toxin region hits (Macrander et al., 2018). With further analysis such as toxin homology in closely related, annotated marine taxa and accounting for …

Soil Respiration Drivers And Challenges In A Changing Environment, Olivia Rae Haas

Theses, Dissertations and Culminating Projects

This study aims to understand post agricultural forested soils (sandy loam, silt loam, and loam) and the influences that drive carbon sequestration and soil respiration flux. Duke Farms in Hillsborough NJ, has historically been utilized as an agricultural zone for farming a variety of crops. Currently the area is protected with a focus on environmental conservation and restoration of the land. This includes getting a better understanding of the forested portions of the property. Understanding the quality of forested areas is vital as they have the potential to act as a carbon sink which can mitigate the effects of climate …

Nanobubble-Enabled Foam Fractionation To Remove Freshwater Microalgae And Microcystin, Yihan Zhang, Lili Li, Shan Xue, Tsung-Ta David Hsu, Anne C. Hurley, Meiyin S Wu, Xuezhi Zhang, Wen Zhang

Department of Biology Faculty Scholarship and Creative Works

Climate change and water pollution intensify algal blooms in natural lakes and reservoirs, leading to significant water quality challenges. Effectively removing excessive microalgae and algal toxins or micropollutants is essential for improving water quality, preserving aesthetic value, and protecting human health. This study evaluated the use of nanobubble-enabled foam fractionation for the removal of Microcystis aeruginosa and three microcystin congeners (MC-LR, MC-RR, and MC-YR). Foam was generated using air nanobubbles combined with surfactants—cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), and chitosan—to enhance foamability and stability. Among the tested surfactants, CTAB exhibited the highest foamability, yielding improved removal performance. When CTAB …

Inferring Genes And Biological Systems Under Selective Pressure In The Secondarily Temperate Cryonotothenioids, Esteban Cabrera

Theses, Dissertations and Culminating Projects

Notothenioid fishes represent one of the most compelling examples of adaptive radiation driven by extreme environmental change, having diversified in the chronically cold waters of the Southern Ocean. While most cryonotothenioids evolved highly specialized cold-adapted characteristics, several secondarily temperate species later re-invaded warmer environments. These lineages provide a rare opportunity to examine evolutionary reversibility and the genomic mechanisms enabling escape from extreme specialization. In this study, we investigate two secondarily temperate species, Dissostichus eleginoides and Champsocephalus esox, comparing their protein-coding orthogroups to background sets from Antarctic cryonotothenioids and basal temperate teleosts. Using phylogeny based evolutionary models (BUSTED, aBSREL, and RELAX) …

A Trajectory Of Zostera Marina (Eelgrass) Ecosystem Recovery: Pre- And Post-Hurricane Sandy Degradation In Barnegat Bay, New Jersey, Edgar A. Medina

Theses, Dissertations and Culminating Projects

Hurricane Sandy struck Barnegat Bay, New Jersey in 2012 and obliterated the benthic marine ecosystem with extensive damage to Zostera marina meadows. Previous genetic assessments of Barnegat Bay Z. marina in 2008 revealed low heterozygosity and low connectivity between populations. The great disruption caused by Hurricane Sandy motivated us to investigate the long-term effects of the storm on the genetic condition of the eelgrass populations in the year 2021. The investigation revealed improvements in heterozygosity, inbreeding, and connectivity. These findings drove us to investigate the genetics of Barnegat Bay eelgrass populations in the intervening years of 2013 and 2017 with …

Behavioral Strategies And Neural Mechanisms Underlying Short-Range Navigation In Teleost Fish, Adelaide Sibeaux, Theresa Burt De Perera, Thomas Mueller, Ronen Segev

Department of Biology Faculty Scholarship and Creative Works

Studies of spatial cognition in teleosts have uncovered a remarkable array of behavioral strategies and neural mechanisms. While there is compelling evidence that short-range navigation in teleost fish is connected to the telencephalon, the rostral part of the forebrain, little is known about the telencephalic regions mediating this behavior. On the cellular level, several neuron types have been identified in specific regions of the telencephalon that respond to different aspects of the fish’s individual position within a given space. To comprehensively understand spatial representation in fish, we must bridge behavioral and neurophysiological studies. Here, we review the behavioral strategies used …

Habitat Determines The Effects Of Wild Ungulate Grazing On Soils: Case Studies From The Iberian Peninsula, Jennifer Adams Krumins, Shih-Chieh Chien, Ramon Perea, Emmanuel Antonio Serrano Ferron, Elena Baraza Ruiz, The Incremento Consortium

Department of Biology Faculty Scholarship and Creative Works

Background and Aims

The bidirectional relationship between plants and their soils can be disrupted by the influence of ungulate grazers. Indeed, herbivores affect plant productivity and above-ground community structure, and plants affect soil health and below-ground community structure. However, the effects of herbivory can be subtle and challenging to address experimentally, even in the case of relatively large ungulate grazers. We attempt to resolve this paradox and experimentally test effects of ungulate grazers in two case studies on the Spanish Iberian Peninsula.

Methods

At the two locations, we experimentally enclosed red deer (Cervus elaphus) at high and hyper …

Phosphorylation Deficient Inducible Camp Early Repressor (Icer) Modulates Tumorigenesis And Survival In A Transgenic Zebrafish (Danio Rerio) Model Of Melanoma, Justin Wheelan, Melissa Rose Spigelman, Angelo Cirinelli, James Reilly, Carlos A. Molina

Department of Biology Faculty Scholarship and Creative Works

Melanoma, the most lethal form of skin cancer, is commonly associated with mutations in the BRAF gene, particularly BRAF^V600E, which drives tumor proliferation via the ERK1/2 signaling cascade. While BRAF inhibitors initially demonstrate efficacy, therapeutic resistance remains a significant challenge. Emerging evidence implicates the cAMP signaling pathway, particularly the cAMP response element-binding protein (CREB) and its repressor, inducible cAMP early repressor (ICER), in melanoma progression and drug resistance. ICER, a transcriptional repressor regulated via Ras/MAPK-mediated phosphorylation and ubiquitination, is degraded in melanoma, undermining its tumor-suppressive role. In a braf^V600E; p53 (loss of function) transgenic zebrafish ( …

Dopaminergic Neurons In The Zebrafish Subpallium Belong To The Extended Medial Amygdala, Daniel Armbruster, Thomas Mueller, Wolfgang Driever

Department of Biology Faculty Scholarship and Creative Works

The amygdala is a heterogeneous, multinuclear telencephalic structure critical for motivated and emotion-related behaviors in vertebrates. In ray-finned fish (Actinopterygii) like the teleost zebrafish, a telencephalic outward-growing process called eversion makes defining amygdaloid territories particularly challenging. Teleosts are also peculiar in that they develop numerous dopaminergic (DA) neurons in the subpallium, while in tetrapods, such populations are less prominent or appear only transiently. To shed light on the organization of the amygdala in teleosts, we pursued an evolutionary developmental approach focusing on the topological origin of subpallial DA neurons. Specifically, we analyzed the distribution of tyrosine hydroxylase (Th) in conjunction …

Differential Regulation Of The Prkacaa Promoter In Zebrafish Melanoma Cell Lines, Maria Fiuk

Theses, Dissertations and Culminating Projects

The Inducible cAMP Early Repressor (ICER) comprises a group of isoforms that function as transcription factors within the cyclic AMP (cAMP) signaling pathway. These isoforms are encoded by the cAMP Response Element Modulator (CREM) gene. The cAMP signaling pathway and its associated transcriptional regulators have been implicated in the development of resistance to BRAF/MEK-targeted therapies in melanoma. Recent findings from our laboratory demonstrated that transgenic ICER expression in a zebrafish melanoma model promotes the formation of aggressive melanomas. Cell lines were established from tumors expressing ICER, as well as from control tumors expressing Green Fluorescent Protein (GFP). To begin characterizing …

Neuronal Tol-1 Integrates Microbial And Metabolic Cues To Modulate Feeding And Aversion In C. Elegans, Gursimran Singh Gujral

Theses, Dissertations and Culminating Projects

Toll-like receptors (TLRs) are key mediators of innate immunity, but emerging evidence suggests that they also play critical roles in the functioning of the central nervous system. This thesis explores the function of TOL-1, the Caenorhabditis elegans homolog of mammalian TLRs, in sensory neurobiology and behavioral responses to nutritive food. In particular, carbon dioxide and ethanol are commonly encountered in microbial environments and can signal metabolically active food sources or potential pathogens. Using tol-1 loss-of-function mutants, neuronal rescue lines, and a combination of molecular, tissue imaging, and behavioral assays, we demonstrate that TOL-1 functions in chemosensory neurons to regulate feeding …

Juvenile And Adult Survival Estimates Of American Kestrels Throughout The Full Annual Cycle In Eastern North America, Mercy Melo, Joseph M. Kolowski, Rebecca A. Mccabe, John A. Smallwood, Allison Cornell, David King, Jean François Therrien

Department of Biology Faculty Scholarship and Creative Works

Measuring survival in a declining species provides the foundation for continued research, demographic modeling, and informed conservation strategies. Although American Kestrel (Falco sparverius) populations have continued to decline since the 1970s, little is known about their survival throughout the full annual cycle, making it difficult to identify factors responsible for these declines. We used radio telemetry to provide the first known-fate estimates of adult kestrel survival, and we present data across breeding stages, and between resident and migratory wintering individuals, in addition to separately modeling fledgling survival. Temporal and spatial stratification of study sites allowed for the investigation of different …

Connecting The Dots Between Narcissism And Fame Appeal: The Role Of Imaginary Audience Fantasies, Dara Greenwood, Qiana Archer, Sydney Ash, Julian Paul Keenan

Department of Biology Faculty Scholarship and Creative Works

Background:
Although prior research has found a significant association between narcissistic tendencies and fame interest, details regarding the particular nature of this relationship remain to be elucidated. The present online survey study (N = 355) replicates and extends prior research examining the links between two subtypes of narcissism and fame appeal. Specifically, we distinguish between grandiose and vulnerable narcissism, as predictors of Visibility, Status, and Prosocial domains of fame appeal. Further, we examine the potential mediating role of imaginary audience fantasies.

Participants and procedure:
Participants were drawn from a student sample (74% female-identifying) aged 18 to 25 who completed an …

Quantifying The Vulnerability Of Smooth Dogfish And Winter Skate To Electromagnetic Fields From Offshore Wind Transmission Cables In The Mid-Atlantic Shelf, Rachel A. Sechrist

Theses, Dissertations and Culminating Projects

The development of offshore wind structures in the northeastern U.S. will contribute to renewable energy goals, but will overlap with many marine species as well as economically important fisheries. High voltage transmission cables from offshore wind farms emit electromagnetic fields (EMFs) that elasmobranchs may detect using sensory organs known as ampullae of Lorenzini. However, sensitivity to EMFs varies by species due to differences in the number and arrangement of pores, the length of subdermal canals within the ampullae of Lorenzini sensory network, and the habitats they reside and regions they forage. For species who reside or forage in benthic regions, …

Neuroimmune Toll-Like Receptor Signaling Modulates C. Elegans Feeding Behavior In A Hunger State-Dependent Manner, Retwika Ganguly

Theses, Dissertations and Culminating Projects

Animals face the risk of encountering pathogenic microbes while searching for nutritive resources. Assessing the interplay between the benefits of nutrition vs. the risk of infection might influence the behavioral regulation of immune processes. In Caenorhabditis elegans (C. elegans), a nematode roundworm, behavioral immunity is partially regulated by the innate immune molecule TOL-1: a homolog of vertebrate Toll-like Receptor (TLR) proteins. TOL-1 influences C. elegans pathogen avoidance behaviors by promoting the development of CO2-detecting chemosensory neurons, which detect a signal linked to the presence of harmful pathogens. While TOL-1′s role in pathogen avoidance is well established, its impact in an …

Toll-Like Receptor Signaling In Neurons Modulates C. Elegans Feeding Behavior In A Hunger State-Dependent Manner, Neel Patel, Joseph Rios, Retwika Ganguly, Cindy Mutafoglu, Nour Shalash, Karla Gallardo, Malak Saleh, John Chahine, Emily Kopecky, Gursimran Gujral, Kamya Shah, Christos Suriano

Department of Biology Faculty Scholarship and Creative Works

Animals face the risk of encountering pathogenic microbes while foraging for resources. Assessing the risk of nutrition vs. infection can result in the behavioral regulation of immune processes. Behavioral immunity in the nematode roundworm Caenorhabditis elegans (C. elegans) is regulated, in part, by the innate immune molecule TOL-1: a homolog of vertebrate Toll-like Receptor (TLR) proteins that influences C. elegans pathogen avoidance behaviors by promoting the development of CO2-detecting chemosensory neurons. While TOL-1′s role in pathogen avoidance is well established, its role in an opposing behavior – foraging – has not been examined. In addition to pathogenic bacteria, preferred food …

No Detected Signal Of The 2022 Epizootic From Multiannual Diadema Antillarum Settlement Data In The Florida Keys, Jason Albrecht

Theses, Dissertations and Culminating Projects

The sea urchin Diadema antillarum is a key coral reef grazer that remains functionally extinct on many Caribbean reefs following a mass mortality event in 1983–84. In 2022, another widespread mortality event, likely caused by a scuticociliate parasite, again decimated D. antillarum at reefs across the Caribbean. Understanding the population-level impacts of this recent epizootic will be important for coral reef conservation efforts. This study examines multi-year larval settlement data from the Florida Keys to determine if the 2022 mortality event reduced larval settlement. Larval settlement was assessed monthly at three long-term monitoring sites (Delta Shoal, Tennessee Reef, and Pickles …

Draft Assembly And Annotation Of The Cuban Crocodile (Crocodylus Rhombifer) Genome, Robert W. Meredith, Yoamel Milián-García, John Gatesy, Michael A. Russello, George Amato

Department of Biology Faculty Scholarship and Creative Works

Objectives: The new data provide an important genomic resource for the Critically Endangered Cuban crocodile (Crocodylus rhombifer). Cuban crocodiles are restricted to the Zapata Swamp in southern Matanzas Province, Cuba, and readily hybridize with the widespread American crocodile (Crocodylus acutus) in areas of sympatry. The reported de novo assembly will contribute to studies of crocodylian evolutionary history and provide a resource for informing Cuban crocodile conservation. Data description: The final 2.2 Gb draft genome for C. rhombifer consists of 41,387 scaffolds (contigs: N50 = 104.67 Kb; scaffold: N50-518.55 Kb). Benchmarking Universal Single-Copy Orthologs (BUSCO) identified 92.3% of the 3,354 genes …

Salt Pollution Reduces Turbidity, Dissolved Organic Matter, And Cyanobacteria In Experimental Vernal Pool Communities, Jared P. Vigil, Matthew S. Schuler

Department of Biology Faculty Scholarship and Creative Works

Anthropogenic activities such as the over-application of road deicers are causing an increase in the concentration of salts in historically fresh waters. Experimental and field investigations demonstrate that freshwater salinization disrupts ecosystem functions and services, causing the death of freshwater organisms and changes to nutrient conditions. Wetland habitats are one system negatively affected by salt pollution, including ephemeral wetlands (vernal pools) that fill with salt-polluted water after snowmelt. In urbanized areas, the degradation of these ecosystems could result in irreversible ecological damage including reduced water quality and a reduction in biodiversity. To investigate the effects of freshwater salinization on vernal …