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2011

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Full-Text Articles in Biology

Landsat Remote Sensing Data As An Alternative Approach For Geological Mapping In Tanzania: A Case Study In The Rungwe Volcanic Province, South-Western Tanzania, E. E. Mshiu Dec 2011

Landsat Remote Sensing Data As An Alternative Approach For Geological Mapping In Tanzania: A Case Study In The Rungwe Volcanic Province, South-Western Tanzania, E. E. Mshiu

Tanzania Journal of Science

Rungwe Volcanic Province (RVP) is mostly covered by extrusive rocks that overlain the Precambrian basement. The use of Landsat data in this area has revealed the need of effective use of these data in geological mapping programs in Tanzania. Landsat band ratios 5/1, 3/7, 5/7 and 5/4 as well as R: G: B composite images 7:4:1, 7:5:4, 1/3:5/7:3/5 and 4/5:6/7:4/6 played an important role in identifying different rock types in the study area. Ratio images managed clearly to distinguish between mafic and felsic rocks whereby two lithological blocks were identified, Block 1 covers a combination of intrusive and metamorphic rocks …


Growth Performance, Yields And Economic Benefits Of Nile Tilapia Oreochromis Niloticus And Kales Brassica Oleracea Cultured Under Vegetable-Fish Culture Integration, A. P. Shoko, A. Getabu, G. Mwayuli, Y. D. Mgaya Dec 2011

Growth Performance, Yields And Economic Benefits Of Nile Tilapia Oreochromis Niloticus And Kales Brassica Oleracea Cultured Under Vegetable-Fish Culture Integration, A. P. Shoko, A. Getabu, G. Mwayuli, Y. D. Mgaya

Tanzania Journal of Science

An experiment was conducted for 210 days to demonstrate the role of vegetable-fish culture integration in the growth, yields and economic benefits of fish and vegetables. Two 200 m2 earthen fishponds were stocked with Nile tilapia Oreochromis niloticus at 20,000 fish fingerlings per hectare. Pond A was fertilized with chicken manure and stocked fish fed on 35% crude protein supplementary diet referred to here as treated fish pond (TFP). Another fish pond was not fertilized and the fish stocked in it did not receive any supplementary diet referred to here as non treated fish pond (NTFP). Twelve vegetable plots of …


Variability In Parasites’ Community Structure And Composition In Cat Fish With Respect To Level Of Pollution In Mwanza Gulf, Lake Victoria, Tanzania, C. J. Mwita Dec 2011

Variability In Parasites’ Community Structure And Composition In Cat Fish With Respect To Level Of Pollution In Mwanza Gulf, Lake Victoria, Tanzania, C. J. Mwita

Tanzania Journal of Science

This study investigated the composition and structure of the parasite communities in Cat fish with respect to levels of water pollution in Lake Victoria. A total of 1071 Clarias gariepinus with mean TL range of 19 to 27 cm were analyzed from three localities in Mwanza Gulf (Kirumba, 298 fish infected with 15 parasite species), Nyegezi (376 fish, with 15 parasites species) and Malimbe (397 fish, with 16 parasites species). Trematodes and nematodes dominated the population with six (35.3%) and five (29.4%) species, respectively. Mean species richness and heteroxenous/monoxenous ratio showed significant changes (Fr = 8.000; p = 0.0046), being …


Host-Parasites Relationship For Lake Victoria Clariid Fishes And Their Parasite Fauna, C. J. Mwita Dec 2011

Host-Parasites Relationship For Lake Victoria Clariid Fishes And Their Parasite Fauna, C. J. Mwita

Tanzania Journal of Science

Host-parasite co-evolutionary relationships for the clariid fish species of the Lake Victoria basin and their parasite fauna was inferred by cytochrome b gene and the 18S rDNA using ‘a strict coevolutionary’ constrained tree method, the results of which revealed a phylogenetic incongruence between the fish hosts and their parasite fauna. The present study further indicated that the sympatric association between the clariid hosts and non-clariid fishes in Lake Victoria makes it possible for the parasites to switch from one host to another and hence the large number of shared parasites observed. The specificity of some of the parasites, e.g. Diplostomum …


Natural Variation In Gestational Cortisol Is Associated With Patterns Of Growth In Marmoset Monkeys (Callithrix Geoffroyi), Aaryn C. Mustoe, Andrew K. Birnie, Andrew V. Korgan, Jonathan Bruce Santo, Jeffrey French Dec 2011

Natural Variation In Gestational Cortisol Is Associated With Patterns Of Growth In Marmoset Monkeys (Callithrix Geoffroyi), Aaryn C. Mustoe, Andrew K. Birnie, Andrew V. Korgan, Jonathan Bruce Santo, Jeffrey French

Psychology Faculty Publications

High levels of prenatal cortisol have been previously reported to retard fetal growth. Although cortisol plays a pivotal role in prenatal maturation, heightened exposure to cortisol can result in lower body weights at birth, which have been shown to be associated with adult diseases like hypertension and cardiovascular disease. This study examines the relationship between natural variation in gestational cortisol and fetal and postnatal growth in marmoset monkeys. Urinary samples obtained during the mother’s gestation were analyzed for cortisol. Marmoset body mass index (BMI) was measured from birth through 540 days in 30- or 60-day intervals. Multi-level modeling was used …


Reverse Protection Assay: A Tool To Analyze Transcriptional Rates From Individual Promoters, Yan O. Zubo, Victor V. Kusnetsov, Thomas Börner, Karsten Liere Dec 2011

Reverse Protection Assay: A Tool To Analyze Transcriptional Rates From Individual Promoters, Yan O. Zubo, Victor V. Kusnetsov, Thomas Börner, Karsten Liere

Dartmouth Scholarship

Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.


Role Of Pseudomonas Produced Hydrogen Cyanide In Biological Control Of Plant-Parasitic Nematodes, Haytham Aly Dec 2011

Role Of Pseudomonas Produced Hydrogen Cyanide In Biological Control Of Plant-Parasitic Nematodes, Haytham Aly

Dissertations

Plant-parasitic nematodes are among the most devastating pests world-wide, causing extensive damage to important agriculture crops. The management of nematodes is challenging. Current methods used for nematode control rely mostly on using chemical nematicides and naturally resistant plants. Because of serious health and environmental problems associated with chemical control and over use of resistant plants resulting in resistant breaking strains of nematodes, alternative nematode-control methods are needed. Several potential nematode-biocontrol agents, including bacteria and fungi, have been studied for their antagonistic effect toward plant-parasitic nematodes. However, a concise picture about the biological agent’s mechanisms used to antagonize nematodes remains elusive. …


The Role Of The Nascent Polypeptide-Associated Complex In Caenorhabditis Elegans, Paul T. Arsenovic Dec 2011

The Role Of The Nascent Polypeptide-Associated Complex In Caenorhabditis Elegans, Paul T. Arsenovic

Masters Theses, 2010-2019

The nascent polypeptide-associated complex (NAC) is a highly conserved protein complex known to play an important role in the development of metazoan organisms, but its molecular function is not well understood. Recent evidence from experiments using Saccharomyces cerevisiae as model supported the hypothesis that the NAC is either a chaperone or a component of the cytosolic chaperone network that interacts with nascent peptides emerging from the ribosome. We tested this model in C. elegans and found that the homologues of the NAC , icd-1 and icd-2, behave like chaperones in the worm. Lack of icd-1 or icd-2 altered the worms …


Effects Of The Exotic Shrub Lonicera Maackii On The Plant Animal Interactions In The Invaded Habitat, Humberto De Paula Dutra Dec 2011

Effects Of The Exotic Shrub Lonicera Maackii On The Plant Animal Interactions In The Invaded Habitat, Humberto De Paula Dutra

Dissertations

Invasive plants are an economical, political, social, and ecological problem. Some invasive species are a serious concern for society because some of them are diseases, some are agricultural pests, and some become major threats to the ecosystems. Most studies with invasive species are limited because they measure only the impact of the invasion via direct pathways. Unfortunately, the potential for non-native plant species to alter ecosystem functions via indirect pathways mediated by interactions with animals just recently became broadly recognized. This research investigates the interactions of Amur honeysuckle with native fauna and how these interactions indirectly affect other trophic levels …


A Floristic Survey Of The Lichens Of The Spring Mountains, Nevada, Usa, Monica W. Proulx Dec 2011

A Floristic Survey Of The Lichens Of The Spring Mountains, Nevada, Usa, Monica W. Proulx

Theses and Dissertations

This thesis is the culmination of a graduate research project involving a floristic survey of the lichens of the Spring Mountain National Recreation Area (SMNRA), Humboldt-Toiyabe National Forest, Nevada. The project was based on extensive collections made between 1997 and 2007 as part of an air pollution biomonitoring program and a baseline established by Larry St. Clair (BYU). The Spring Mountains are a sky island mountain range in the Mojave Desert located less than an hour northwest of Las Vegas. A floristic survey of the lichen communities in the Spring Mountains represents a major addition to our understanding of the …


Reproducible Hairy Root Transformation And Spot-Inoculation Methods To Study Root Symbioses Of Pea, Scott R. Clemow, Lindsey Clairmont, Lene H. Madsen, Frédérique C. Guinel Dec 2011

Reproducible Hairy Root Transformation And Spot-Inoculation Methods To Study Root Symbioses Of Pea, Scott R. Clemow, Lindsey Clairmont, Lene H. Madsen, Frédérique C. Guinel

Biology Faculty Publications

Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodulating pea mutants, 2) a rhizobial inoculation method which allows the study of early cellular events giving rise to nodule primordia, and 3) a targeted fungal inoculation method which allows us to study short segments of mycorrhizal roots assured to be infected. These tools are certain to advance our knowledge of pea root symbioses.


Discrimination And Aggregative Patterns Among And Between Populations Of Entamoeba Spp., Katelyn M. Higgins Dec 2011

Discrimination And Aggregative Patterns Among And Between Populations Of Entamoeba Spp., Katelyn M. Higgins

Honors Senior Capstones and Theses

Kin recognition facilitates the evolution of cooperation in animals, but its relevance in microorganisms and their behavior toward relatives remains unclear. The Entamoeba lineage constitutes an ideal model to determine the behavioral and signaling cues needed for aggregative preference. Chemical cues have been reported for E. histolytica but not E. dispar, suggesting cell-communication between con-specifics and behavioral differences with relevance for disease. Entamoeba varieties have been reported as ‘morphologically undistinguishable’. Two strains of E. invadens have been isolated from different hosts (VK-1: NS - Varanus komodoensis and IP1- serpentes) but were classified within the same ‘species’. Trophozoites …


Selectivity Of Prey Capture Based On Prey Size In The Venus Fly Trap (Dionaea Muscipula Ellis), Jessica Humenik Dec 2011

Selectivity Of Prey Capture Based On Prey Size In The Venus Fly Trap (Dionaea Muscipula Ellis), Jessica Humenik

Honors Theses

Venus fly traps (Dionaea muscipula Ellis) are carnivorous plants that live in nutrient poor soils and must digest insects to supplement their diets. When a Venus fly trap captures an insect, the plant's traps do not fully close for several minutes, which may allow small prey to escape. It would be beneficial for the plant to consume a large, nutrient-rich prey item as opposed to a small prey item. We tested the hypothesis that Venus fly traps select larger prey by offering plants small and large crickets. A Kruskal-Wallis test and Wilcoxon signed rank test were used to test significance …


Lesson Not Learned: Deepwater Horizon Research And Media Coverage Exposes Gaps In Knowledge And Risky Protocol Within The Oil Industry, Lauren Haller Dec 2011

Lesson Not Learned: Deepwater Horizon Research And Media Coverage Exposes Gaps In Knowledge And Risky Protocol Within The Oil Industry, Lauren Haller

Honors Theses

An insatiable thirst for oil has led poorly coordinated, risk-prone megasystems deeper into the ocean in search of new oil reserves. Profit-driven agendas at the corporate level have a top-down effect within these megasystems. Cost-cutting and risk-downplaying leaves the field employees unprepared to handle emergencies. A series of costly mistakes led to the 2010 BP Deepwater Horizon oil spill, which caused extensive damage to an already fragile ecosystem in the Gulf of Mexico. The wealth and political influence of the oil industry overpowers lax regulatory agencies and legislation-even though media and research has exposed frustrating parallels between the Deepwater Horizon …


The Involvement Of 26s Proteasome Complex In Selenium Toxicity, Melissa Sabbagh Dec 2011

The Involvement Of 26s Proteasome Complex In Selenium Toxicity, Melissa Sabbagh

Honors Theses

Plants that hyperaccumulate elements like selenium to potentially toxic levels may use proteasome complexes to reduce toxicity. The 26S proteasome complex may be a pathway that these plants take to rid themselves of selenium toxicity by destroying damaged proteins caused by selenium. A method to test this hypothesis is to use a hyperaccumulator of selenium Stanleya pinnata and nonhyperaccumulators Populous tremula and Arabdopsis thaliana to evaluate their proteasome abundance without and with selenium. To compare these species western blots were made to show the differences in proteasome abundance. Also to compare the amount of oxidized and an ubiquinated protein in …


Aging Of Florida Blue Crabs, Callinectes Sapidus, Through The Biochemical Extraction Of Lipofuscin, Claire Elizabeth Crowley Dec 2011

Aging Of Florida Blue Crabs, Callinectes Sapidus, Through The Biochemical Extraction Of Lipofuscin, Claire Elizabeth Crowley

USF Tampa Graduate Theses and Dissertations

The blue crab, Callinectes sapidus, represents an ecologically and economically important component of marine and estuarine ecosystems. In Florida, blue crab landings accounted for $9.6 million dollars during the 2010 fishing season. Accurate stock assessments for this valuable fishery are essential. Age is a critical biological component of accurate stock assessments; however, blue crabs and other crustaceans are especially difficult to age because of the complex nature of discrete growth. Biochemical extraction of an aging pigment, lipofuscin, was developed using blue crab eyestalks. The current study investigated the effects of freezing preservation on lipofuscin extracts and examined whether the …


Elucidating Matrix Protein Import And Beta-Oxidation Pathways In Plant Peroxisomes, Bibi Rafeiza Khan Dec 2011

Elucidating Matrix Protein Import And Beta-Oxidation Pathways In Plant Peroxisomes, Bibi Rafeiza Khan

Dissertations

Plant peroxisomes function in fatty acid β-oxidation, jasmonic acid (JA) synthesis, and conversion of indole-3-butyric acid (IBA) to indole-3-acetic acid (IAA). Peroxisomes lack genetic material; proteins required for peroxisomal processes are imported posttranslationally. The PEX5 and PEX7 receptors import proteins containing one of two peroxisomal targeting signals (PTS1 or PTS2). I took a genetic approach to better understand protein import and to elucidate the role of Acyl-CoA Oxidase (ACX) enzymes in the model plant Arabidopsis thaliana. ACX enzymes catalyze the first step in β-oxidation. There are six ACX genes in Arabidopsis and each ACX enzyme acts on specific chain-length targets, …


Species Level Differences In The Ecology Of Two Neotropical Tadpole Species: Responses To Nonlethal Predators And The Roles Of Competition And Resource Use, Zacharia Costa Dec 2011

Species Level Differences In The Ecology Of Two Neotropical Tadpole Species: Responses To Nonlethal Predators And The Roles Of Competition And Resource Use, Zacharia Costa

Theses and Dissertations

Closely related species at the same trophic level are often considered to be ecologically equivalent. However, it is clear that individuals species can have unique functional roles that drive community and ecosystem processes. In this study we examine the growth responses of two Neotropical hylid tadpole species, Agalychnis callidryas and Dendropsophus ebraccatus, to intraspecific and interspecific competition. We also look at density-dependent effects of each on phytoplankton, periphyton and zooplankton, as well as their responses to a caged dragonfly predator through ontogeny. Intraspecific competition affected both species similarly, and their effects on resources were qualitatively similar but quantitatively different. Predators …


The Direct Role Of Enzyme Hydrolysis On Ammonium Regeneration Rates In Estuarine Sediments, Afonso C. Souza, Tamara K. Pease, Wayne S. Gardner Dec 2011

The Direct Role Of Enzyme Hydrolysis On Ammonium Regeneration Rates In Estuarine Sediments, Afonso C. Souza, Tamara K. Pease, Wayne S. Gardner

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Benthic ammonium (NH4 +) regeneration in coastal marine sediments has a fundamental role in nitrogen (N) cycling and N supply to primary producers. Nitrogen regeneration involves benthic microbial mineralization of organic-N, which, in turn, depends on protein hydrolysis. These processes were examined in Aransas Bay (Texas, USA) sediments by monitoring NH4 + evolution as a function of enzyme activity in controlled sediment slurries. Casein and tannic acid were added to evaluate the direct role of aminopeptidase on NH4 + production and the effects of a polyphenolic enzyme inhibitor, respectively. Casein additions increased the NH4 + concentration from 19 ± 0.3 …


Evaluation Of Brook Trout (Salvelinus Fontinalis) Introductions And Re-Introductions Into Four Virginia Blue Ridge Mountain Streams, Michael Isel Iii Dec 2011

Evaluation Of Brook Trout (Salvelinus Fontinalis) Introductions And Re-Introductions Into Four Virginia Blue Ridge Mountain Streams, Michael Isel Iii

Theses and Dissertations

Approximately 100 brook trout were transplanted into each of four Virginia streams in September 2008. Garth Run and Kinsey Run were re-introductions, and Wildcat Hollow and Sweet Run were new introductions. Single pass electrofishing (EF) surveys were conducted with a backpack electrofisher five times during the study. Selected trout > 70 mm but < 100 mm and all trout > 100 mm received a Biomark® 134.2 kHz passive integrated transponder (PIT) tag to identify individual fish upon recapture. Trout were present in all streams almost three years post introductions. Catches decreased across the first four surveys. Young of year catch rates severely decreased from June 2009 (n = …


Characterization Of A Novel Gef Kiaa1362, Emily Roach Dec 2011

Characterization Of A Novel Gef Kiaa1362, Emily Roach

Honors Capstones

Capstone submitted as a graduation requirement for the BSU Honors Program.


Factors Controlling Dissolved Organic Carbon Lability And Ecological Fate In The East Branch Swift River, Massachusettes, Hall Eric Dec 2011

Factors Controlling Dissolved Organic Carbon Lability And Ecological Fate In The East Branch Swift River, Massachusettes, Hall Eric

Theses and Dissertations

Fluvial systems have been estimated to transform, transport, or store 2.75 petagrams (Pg) of Organic Carbon (OC) per year. Although approximately 1Pg per year of terrestrial carbon is fluxed to the atmosphere through inland waters, little is known about the factors regulating its eventual ecological fate. 28 day lability incubations were conducted concurrent with the measurement of several environmental parameters including discharge, nutrient concentration, DO13C, and DOC:DON at several sites along Bigelow Brook and the East Branch of the Swift River, Massachusetts. Temporal and spatial variation of DOC, DOC:DON and DO13C were explored. Two distinct DOC consumption rates, short and …


Global Change, Global Trade, And The Next Wave Of Plant Invasions, Bethany A. Bradley, Dana M. Blumenthal, Regan Early, Edwin D. Grosholz, Joshua J. Lawler, Luke P. Miller, Cascade J.B. Sorte, Carla M. D'Antonio, Jeffrey M. Diez, Jeffrey S. Dukes, Ines Ibanez, Julian D. Olden Dec 2011

Global Change, Global Trade, And The Next Wave Of Plant Invasions, Bethany A. Bradley, Dana M. Blumenthal, Regan Early, Edwin D. Grosholz, Joshua J. Lawler, Luke P. Miller, Cascade J.B. Sorte, Carla M. D'Antonio, Jeffrey M. Diez, Jeffrey S. Dukes, Ines Ibanez, Julian D. Olden

Faculty Publications, Biological Sciences

Many non-native plants in the US have become problematic invaders of native and managed ecosystems, but a new generation of invasive species may be at our doorstep. Here, we review trends in the horticultural trade and invasion patterns of previously introduced species and show that novel species introductions from emerging horticultural trade partners are likely to rapidly increase invasion risk. At the same time, climate change and water restrictions are increasing demand for new types of species adapted to warm and dry environments. This confluence of forces could expose the US to a range of new invasive species, including many …


Pulmonary Diffusional Screening And The Scaling Laws Of Mammalian Metabolic Rates, Chen Hou, Michael Mayo Dec 2011

Pulmonary Diffusional Screening And The Scaling Laws Of Mammalian Metabolic Rates, Chen Hou, Michael Mayo

Biological Sciences Faculty Research & Creative Works

Theoretical considerations suggest that the mammalian metabolic rate is linearly proportional to the surface areas of mitochondria, capillary, and alveolar membranes. However, the scaling exponents of these surface areas to the mammals' body mass (approximately 0.9-1) are higher than exponents of the resting metabolic rate (RMR) to body mass (approximately 0.75), although similar to the one of exercise metabolic rate (EMR); the underlying physiological cause of this mismatch remains unclear. The analysis presented here shows that discrepancies between the scaling exponents of RMR and the relevant surface areas may originate from, at least for the system of alveolar membranes in …


What Are The Effects Of Raw Vs. Pasteurized Milk Consumption On Growth Rate And Fertility In A Colony Of Mice?, Kari Sholing, Lindsay Ganong, Amy Olson Dec 2011

What Are The Effects Of Raw Vs. Pasteurized Milk Consumption On Growth Rate And Fertility In A Colony Of Mice?, Kari Sholing, Lindsay Ganong, Amy Olson

Nutrition Student Work

Interest in consuming unprocessed foods has led to assumptions that raw milk has health benefits over pasteurized milk. This study was designed to evaluate the belief that raw milk is nutritionally superior. Eighteen mice were randomly assigned to one of two groups: raw milk or pasteurized milk. Mice were grouped into breeding trios and given a fresh supply of milk every four hours between 7 a.m. and 11 p.m. for 55 days. Milk consumption was tracked at each feeding by measuring the amount of milk that was provided and the amount of milk that was left from the previous feeding. …


Characterization Of An S-Layer-Like Protein And Select Antimicrobial Factors Of The Novel Species Lactobacillus Coleohominis, Nicole Ann Marie Ayala Dec 2011

Characterization Of An S-Layer-Like Protein And Select Antimicrobial Factors Of The Novel Species Lactobacillus Coleohominis, Nicole Ann Marie Ayala

Theses & Dissertations

Lactobacilli in the vaginal tract have been reported be protective against infections with vaginal pathogenic microorganisms through the production of antimicrobial factors, competition for adherence to vaginal epithelium, and coaggregation with pathogens to aid in their clearing. In addition, the antimicrobial factors produced by certain species of lactobacilli have even been shown to interrupt the replication or alter the function of select viruses. Vaginal lactobacilli have been investigated for their antimicrobial characteristics, but little is known about the novel species L coleohominis. recently described in vaginal isolates (Nikolaitchouk et al. 2001). It was hypothesized that L coleohominis, found …


Comparison Of Disturbance Impacts To And Spatial Distribution Of Biological Soil Crusts In The Little San Bernardino Mountains Of Joshua Tree National Park, California, Nicole Pietrasiak, Jeffrey R. Johansen, Tasha Ladoux, Robert C. Graham Dec 2011

Comparison Of Disturbance Impacts To And Spatial Distribution Of Biological Soil Crusts In The Little San Bernardino Mountains Of Joshua Tree National Park, California, Nicole Pietrasiak, Jeffrey R. Johansen, Tasha Ladoux, Robert C. Graham

Biology

Biological soil crust ecology in the hot Mojave Desert is poorly understood with regard to crust distribution and abundance, as well as the impacts of trampling disturbance on crust development. Our objective was to study biological soil crusts in 2 areas of differing disturbance pressures in the high desert region of Joshua Tree National Park, California, with respect to visible crust cover and frequency, chlorophyll a, and soil stability. Impacts on biological soil crusts from 2 disturbance regimes, historic grazing and recent high knot traffic, were compared using a disturbance indicator. In addition, we measured a suite of abiotic and …


Utilizing Fishermen Knowledge And Expertise: Keys To Success For Collaborative Fisheries Research, Noëlle Yochum, Richard M. Starr, Dean Wendt Dec 2011

Utilizing Fishermen Knowledge And Expertise: Keys To Success For Collaborative Fisheries Research, Noëlle Yochum, Richard M. Starr, Dean Wendt

Biological Sciences

Collaborative fisheries research provides a mechanism for integrating the unique knowledge, experience, and skills of fishermen and scientists. It is a joint intellectual endeavor that begins with the inception of a project and continues until its final stages, with each group having mutual investment in—and ownership of— the project. Collaborative fisheries research promotes communication and trust among fishermen, scientists, and managers and can provide much-needed scientifically valid data for fisheries management. It can enhance federal and state management data collection programs, which span broad sections of coastline, by increasing the ability to detect changes in local metapopulations that may be …


Suppression Of Phospholipase Dγs Confers Increased Aluminum Resistance In Arabidopsis Thaliana, Xuemin Wang, Jian Zhao, Cunxi Wang, Mohamed Bedair, Ruth Welti, Lloyd Sumner, Ivan Baxter Dec 2011

Suppression Of Phospholipase Dγs Confers Increased Aluminum Resistance In Arabidopsis Thaliana, Xuemin Wang, Jian Zhao, Cunxi Wang, Mohamed Bedair, Ruth Welti, Lloyd Sumner, Ivan Baxter

Biology Department Faculty Works

Aluminum (Al) toxicity is the major stress in acidic soil that comprises about 50% of the world's arable land. The complex molecular mechanisms of Al toxicity have yet to be fully determined. As a barrier to Al entrance, plant cell membranes play essential roles in plant interaction with Al, and lipid composition and membrane integrity change significantly under Al stress. Here, we show that phospholipase Dγs (PLDγs) are induced by Al stress and contribute to Al-induced membrane lipid alterations. RNAi suppression of PLDγ resulted in a decrease in both PLDγ1 and PLDγ2 expression and an increase in Al resistance. Genetic …


Genotypic And Phenotypic Variation In Pseudomonas Aeruginosa Reveals Signatures Of Secondary Infection And Mutator Activity In Certain Cystic Fibrosis Patients With Chronic Lung Infections, Ashley E. Warren, Carla M. Boulianne-Larsen, Christine B. Chandler, Kami Chiotti, Evgueny Kroll, Scott R. Miller, Francois Taddei, Isabelle Sermet-Gaudelus, Agnes Ferroni, Kathleen Mcinnerney, Michael J. Franklin, Frank Rosenzweig Dec 2011

Genotypic And Phenotypic Variation In Pseudomonas Aeruginosa Reveals Signatures Of Secondary Infection And Mutator Activity In Certain Cystic Fibrosis Patients With Chronic Lung Infections, Ashley E. Warren, Carla M. Boulianne-Larsen, Christine B. Chandler, Kami Chiotti, Evgueny Kroll, Scott R. Miller, Francois Taddei, Isabelle Sermet-Gaudelus, Agnes Ferroni, Kathleen Mcinnerney, Michael J. Franklin, Frank Rosenzweig

Biological Sciences Faculty Publications

Evolutionary adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung is limited by genetic variation, which depends on rates of horizontal gene transfer and mutation supply. Because each may increase following secondary infection or mutator emergence, we sought to ascertain the incidence of secondary infection and genetic variability in populations containing or lacking mutators. Forty-nine strains collected over 3 years from 16 patients were phenotyped for antibiotic resistance and mutator status and were genotyped by repetitive-sequence PCR (rep-PCR), pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). Though phenotypic and genetic polymorphisms were widespread and clustered more strongly within than …