Biology Commons^™

Hagfish Intermediate Filament Proteins: From A Natural Protein System To Functional Biomaterials, Oran Wasserman, Kristin K. Durrant, Katee Stromberg, Brianne E. Bell, Paula E. Oliveira, Justin A. Jones

Biology Student Research

Sourcing protein-based biomaterials directly from nature presents persistent challenges in scalability, processing, and batch-to-batch variability. Recombinant engineering has focused largely on spider silks, and hagfish intermediate filament (HIF) proteins remain a comparatively unexplored alternative, with small non-repetitive subunits that have been produced full-length at high yield. This review examines the biology of naturally produced HIF protein threads from the Pacific hagfish, Eptatretus stoutii, advances in recombinant expression and purification, and their translation into functional biomaterials. We first describe the tripartite domain architecture and biochemical classification of the threonine-rich α- and γ-keratin-like subunits, and then the defensive biomechanics of HIF …

Building Appreciation For Natural History Collections From Biological Collection Data., Jamie Jensen, Seth Bybee

ScholarsArchive Data

These data represent the pre- and post-survey responses by students in an undergraduate, non-majors biology course given before and after a unit on Natural History Collections. We administered the Revised New Ecological Paradigm (R-NEP; Dunlap et al., 2000) survey which measures pro-environmental attitudes using 15 Likert-scale items.

Global Stabilization Of The Mitochondrial Proteome Is Associated With Extreme Anoxia Tolerance In Austrofundulus Limnaeus Ws40ne Cells, Chelsea Hughes, Meranda M. Corona, Dietmar Kültz, Jason E. Podrabsky

Biology Faculty Publications and Presentations

Background: Austrofundulus limnaeus is an extremophile vertebrate native to small temporary ponds of Venezuela. Embryos of A. limnaeus must survive variable and often extreme conditions, including long periods of anoxia. Neuroepithelial cells derived from these embryos, WS40NE cells, provide a unique tool to understand how the proteome changes in response to anoxic stress. Results Using label-free proteomics, 19,604 peptides and 3487 proteins were quantified in normoxic, 4d anoxic, and 24 h recovery WS40NE cells. Of these, 2612 proteins (74.9%) were statistically significantly differentially abundant in at least one comparison: 1988 comparing normoxia to 4 days anoxia (57.0%), 923 comparing 4 …

Parents Don’T Dictate The Menu: Lack Of Strong Host-Specific Parental Effects May Help Maintain Dietary Generalism, Emma J. Sellers, Mayra C. Vidal, Dhaval K. Vyas, Mykaela Tanino-Springsteen, Gina M. Wimp, Mattheau S. Comerford, Mariana Abarca, Shannon M. Murphy

Biological Sciences: Faculty Publications

The environment or experiences of a parent generation can impact the fitness of the next generation, a phenomenon known as parental effects. While a high-quality diet can directly benefit an individual and a low-quality diet can reduce fitness, a parental effect occurs only when there is a transgenerational response to these stimuli that manifests in the individual’s offspring. In dietary generalists, a parental effect could play an important role for offspring performance if the species experiences considerable variation in quality across host plants; posi- tive parental effects may result from host matching between parents and offspring on high-quality host plants, …

Tarantulas Can Use Black And White Cues In A Discrimination Learning Task, Jordan Kolinski, Becky Hansis O'Neill, Amiee Dunlap

Undergraduate Research Symposium

Animals use vision to perceive light, shapes, colors, and movement. Many animals that have poor vision are understudied. The purpose of this experiment was to study color vision in tarantulas (Brachypelma hamorii). Researchers have found genes associated with color vision tarantulas, but no behavioral experiments have been done on this topic. Previous research has also shown that tarantulas can see polarized light. In this experiment, we wanted to know if tarantulas could use visual cues to move away from a noxious stimulus - vibration. There were twelve tarantulas being tested in this experiment for between twelve and fifteen …

Relationship Between Morphological And Behavioral Laterality In Astyanax Mexicanus, Allison Lendman, Michael Tobler, Erik Johnson

Undergraduate Research Symposium

Populations within the Astyanax genus have experienced extreme differences in environment, leading to distinct morphological adaptations. The cave populations of these fish are eyeless and have acquired fixed, cranial asymmetries. We explored a possible relationship between this physical asymmetry and behavioral laterality. We tested 28 adult fish, 14 male and 14 female, from the Pachón cave in controlled behavioral trials. We found that both sexes demonstrated behavioral laterality, observed as a consistent left-turn bias in these trials. This supports the hypothesis that these populations display behavioral laterality that is correlated to their physical asymmetries. Ongoing research comparing behavioral patterns in …

Building Genetic And Culturing Tools For The Understudied Soil Bacterium E. Flagellatus, Aaron Butler, Brenda Pratte, Lon Chubiz

Undergraduate Research Symposium

Soil bacteria play essential roles in nutrient cycling, yet many abundant groups remain poorly characterized due to challenges in cultivation and genetic manipulation. One such organism is Edaphobacter flagellatus, a member of the Acidobacteriota frequently detected in soil environments but lacking well-developed experimental tools. To address this limitation, our laboratory has generated a genome-wide mutant library using the Tn5 transposon, enabling random disruption of genes across the population.

This project aims to establish culture conditions necessary to support the use of this library for functional genomic studies. In particular, defined minimal media can impose selective pressures that reveal …

Winter Contents Of Tree Cavities In Oak Woodland Habitats, Lucy V. Roth, Addy Yoder, Jalene M. Lamontagne Phd

Undergraduate Research Symposium

Woodpeckers create cavities in dead and decaying trees and branches of live trees, many of which are used by a range of other animals, and are thus called “ecosystem engineers”. A species of conservation concern in the midwest is the red-headed woodpecker (RHWO). RHWO in the upper midwest are facultative migrators meaning some individuals stay in their territories over winter and others migrate south. Most tree-cavity research is done during the breeding season, leading to a knowledge gap of their use in the non-breeding season; therefore, studying them in winter could help us understand their ecological impact. RHWO require food …

Nontarget Analysis Of The Volatile And Semivolatile Chemical Profile Of Wildland Firefighter Gear: Insight Into Occupational Exposure And Health Implications, Patrica Ivy Agorsor, Husam Kafeenah, Margaret D. Taiwo, Robin M. Verble, Michael O. Eze

Biological Sciences Faculty Research & Creative Works

Prescribed fire releases a complex mixture of volatile and semivolatile organic compounds to the environment. Firefighters are directly exposed to these hazardous mixtures through occupational activities. This study investigated the volatile and semivolatile chemical contamination profile of firefighter protective gear and the effectiveness of laundering as a decontamination strategy. Our results revealed that firefighter gear accumulated a broad spectrum of combustion-derived contaminants, predominantly composed of polycyclic aromatic hydrocarbons and derivatives, phenolic compounds, and aromatic hydrocarbons. Representative compounds identified included toluene, biphenyl, naphthalene, 1-methynaphthalene, 2-methylnaphthalene, acenaphthene, phenol, alkylated phenols, and chlorinated phenols. Quantitative analysis revealed representative combustion-derived compounds at concentrations (mean …

Dr. Contrarian: A Persona-Driven Conversational Ai Agent For Coaching Argumentative Reasoning In Infectious Disease Classroom Debates, Khayriyyah Mohd Hanafiah

Biology Faculty/Staff Publications

Critical thinking and persuasive communication are essential for biology graduates navigating socioscientific dilemmas. While debate pedagogy fosters problem-solving, autonomy, competence, and relatedness aligned with adult learning and self-determination theories, inexperienced students often struggle with the cognitive load and anxiety from this format. To bridge this gap, “Dr. Contrarian,” a persona-based conversational AI agent, was developed to function as an adversarial coach during debate preparation. Survey data (n = 21) adapted from the Technology Acceptance Model show that 85.7% of students found the tool easy to use, and 71.4% would use it again. Thematic analysis of metacognitive reflections suggests that while …

Broadening The Community Of Nudibranch Enthusiasts Through Multilingual Educational Programming To Increase Climate Advocacy Engagement, Richelle Li Tanner, Cintya Felix Mendivil, Ashley Lam, Lorena Muñoz, Micah Kim, Elena G. Morales Poot, Gabrielle Keeler-May

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Environmental literacy and advocacy for environmental protections are essential components of responsible stewardship. For coastal communities, stewardship requires knowledge of ocean processes and the biological communities living within these ecosystems. While many studies highlight difficulties in engaging American audiences in environmental concern and stewardship due to hyper-individualistic societal values, tidepooling is one recreational pathway to coastal community engagement that strengthens sense of place, and therefore, responsibility to protect natural resources. We sought to broaden the tidepooling community to include more diverse voices through a participatory science program with inland city-dwelling, multilingual, and non-English speaking adults. Using nudibranchs as an environmental …

Cultivating Collaboration In Biomolviz: Key Attributes For Building A Stem Educator Community Of Practice, Pamela S. Mertz, Charmita Burch, Roderico Acevedo, Josh T. Beckham, Didem Vardar-Ulu, Kristin M. Fox, Lauren A. Genova, Rachel M. Mitton-Fry, Swati Agrawal, Daniel R. Dries, Kristen Procko

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

BioMolViz is an educational community of practice (CoP) focused on creating resources to improve biomolecular visual literacy instruction and assessment. The community of life science instructors developed in three phases, beginning with a small Core Team that emerged from a conference session and established the domain of interest for the CoP. In-person workshops, supported by short-term funding, drove the initial expansion of the community and the development of shared activities in Phase 2. This CoP was sustained through intentional choices about how members interact, collaborate, and share work. As the community matured in Phase 3, it was supported by longer-term …

Patterns Of Autonomous And Conditional Neural Specification For The Brain And Ventral Nerve Cord In Annelida, Nicole B. Webster, Johnny A. Davila-Sandoval, Allan M. Carrillo-Baltodano, Skyler Duda, B. Duygu Özpolat, Neva P. Meyer

Biology

Evolution of central nervous systems is a long-debated topic. Similar mechanisms of conditional neural specification linked to dorsal-ventral (D–V) axis formation have been used to support homology across several taxa. We tested for autonomous versus conditional neural specification in two distantly related annelids, Capitella teleta and Platynereis dumerilii, using blastomere isolations and ablations. Our results demonstrate autonomous specification of anterior neural tissue in isolated first-quartet (1q) micromeres for both annelids. In both annelids, partial larvae derived from isolation of the somatoblast, micromere 2d, also resulted in the formation of trunk neural tissue (ventral nerve cord or VNC), suggesting that this …

Insect Monitoring Without Pitfalls: Seven Steps For Robust Insect Sensing Systems, Jamie Alison, Luca Pegoraro, Jarrett Blair, Yuval Cohen, Birgen Haest, Jacob Idec, Jacob Kamminga, Jenna Lawson, Meng Li, Leandro Aparecido Do Nascimento, Charlotte L. Outhwaite, Benjamin Rutschmann, Maximilian Sittinger, Mariana Abarca, Et Al

Biological Sciences: Faculty Publications

1. Data shortages fuel controversy about an ongoing insect biodiversity crisis. Insects are immensely diverse and functionally critical for ecosystems, yet data on their trends remain patchy and biased. Sensors, ranging from camera traps and acoustic recorders to weather radar stations, are set to transform data collection in entomol- ogy. Meanwhile, AI models that extract biological information from sensors are improving at a startling rate.

2. Realising the potential of automated monitoring means progressing from proof- of-concept studies to scalable insect sensing systems. However, stakeholders face severe operational challenges when adopting a growing suite of sensors, models and protocols for …

Bryophyte Community Composition In A Second-Order Tropical Stream At The Luquillo Experimental Forest, Puerto Rico, Cecilia Sahnow, Jean P. González-Crespo, Amelia Merced

Biology Faculty Publications and Presentations

Streams are important ecosystems that harbor a high diversity of plant species including bryophytes. Studies suggest streams can function as refugia and/or corridors that buffer against the effects of disturbances. In this study, we sampled five sites along a second-order tropical stream in the Luquillo Experimental Forest. We described the riparian bryophyte community composition in conjunction with the physical structure of the stream to understand what environmental factors influence bryophyte composition in the stream. We identified 56 bryophytes, 33 liverworts and 23 mosses; and we recorded cyanobacteria associated with over half of the species of bryophytes. Rocks were the most …

Assessing The Evolving The Culture Of Biology Program Through The Lens Of The Teacher-Centered Systemic Reform Model, Diyala S. Shihadih, Elizabeth Runkel Baez, Mitra Asgari, Justin Berl, Adam J. Chouinard, Stephanie J. Gutzler, Kaleb K. Heinrich, Eden Riordan, Star W. Lee, Erin E. Shortlidge

Biology Faculty Publications and Presentations

There are persistent national calls to advance evidence-based instructional practices to increase the number of students graduating with science degrees. Evolving the Culture of Biology is a program designed to improve undergraduate education by prioritizing graduate teaching assistant teaching professional development (TA-TPD). Guided by the Teacher-Centered Systemic Reform (TCSR) model, this study examined how TA TPD practitioners’ (participants) attitudes, personal factors, institutional contexts, and instructional practices shape TA-TPD reform outcomes. Using a pre-program survey and end-of-year semi-structured interviews, we gained insight into participants’ experiences as they developed and implemented reformed TA-TPD. We investigated how TCSR components differentially influenced participant progress, …

Insights Into Longevity And Virus-Driven Adaptation From Myotis Bat Genomes, Juan M. Vazquez, M. Elise Lauterbur, Saba Mottaghinia, Léa Gaucherand, Sarah Maesen, Michael Singer, Sarah Villa, Melanie Bucci, Devaughn Fraser, Genavieve Gray-Sandoval, Zeinab R. Haidar, Melissa Han, William Kohler, Tanya Lama, Et Al

Biological Sciences: Faculty Publications

The genus Myotis is one of the largest clades of bats, and it exhibits some of the most extreme variation in lifespans among mammals, alongside unique adaptations to viral tolerance and immune defence^1–3. Here, to study the evolution of these phenotypes, we generated cell lines and near-complete genome assemblies for eight closely related Myotis species. Using genome-wide screens of positive selection, analyses of structural variation and functional experiments in primary cells, we identify patterns of adaptation contributing to longevity, cancer resistance and viral interactions. We demonstrate distinct modes of adaptation to DNA and RNA viruses compared with all …

The Role Of Seed Mix Characteristics And Soil Amendments During Post-Agricultural Prairie Restoration, Valerie N. Thurston, Michaela J. Woods, Madelaine Gregory, Mary C. Lloyd, Ryan W. Mcewan

Biology Faculty Publications

Introduction: Prairie restoration on degraded lands may be influenced by seed mix characteristics, including species richness and legume establishment. Soil amendments may facilitate restoration; however, the practical usefulness of techniques such as applications of whole soil and leaf mulch remains relatively unclear.

Objectives: We assessed whether increasing species richness and legume content in seed mixes enhances prairie restoration success and examined how soil amendments interact with seed mix composition to influence cover, species richness, diversity, and legume establishment.

Methods: We conducted a field experiment on a 12-ha post-agricultural site in Ohio, U.S.A. Twenty 50 × 50 m plots received one …

Hydrogels For Local Drug Delivery In Biofilm-Associated Periprosthetic Joint Infection: Current Progress And Future Directions, Karolina Kraus, Pawel Mikziński, Bindu Subhadra, Emil Paluch

Faculty Publications - Biological Sciences

Periprosthetic joint infection (PJI) remains one of the most serious complications of arthroplasty, largely due to the formation of microbial biofilms on implant surfaces. Biofilm-associated infections exhibit increased tolerance to antimicrobial therapy and host immune responses, making eradication difficult and often requiring repeated surgical interventions. Consequently, there is a growing need for effective local therapeutic strategies capable of delivering high concentrations of antimicrobial agents directly to the site of infection while minimizing systemic toxicity. Hydrogels have emerged as promising drug delivery platforms for the management of biofilm-associated PJI. Their biocompatibility, injectability, high water content, and tunable physicochemical properties enable controlled …

Complete Genome Sequence Of Strain Dk8, A Thermoacidophilic Archaeon From Lassen Volcanic National Park, Jonathan Abshier, Kenneth Stedman

Biology Faculty Publications and Presentations

Here, we report the genome sequence of strain DK8, a thermoacidophilic archaeal isolate from the Devil's Kitchen geothermal area of Lassen Volcanic National Park, California, USA. The genome is 2,783,064 bp, with a guanine-cytosine content of 35.02%. Strain DK8 is most closely related to L.S.2.15.

Parental Thermal Environment Affects Offspring Resilience To Winter And Spring Weather Anomalies, Ruzhuo Chen, Mariel Dunn, Caroline A. Curtis, Mariana Abarca

Biological Sciences: Faculty Publications

Recent increases in the frequency and intensity of thermal anomalies can result in recurrent stressful weather events. Among annual insects, this phenomenon may expose parents and offspring to a succession of different weather anomalies (e.g. summer heatwaves and late spring frosts). Parental stress can alter offspring physiology and performance, potentially affecting their tolerance of environmental variation. We investigated the transgenerational effects of thermal anomalies on a univoltine wetland butterfly. We induced parental thermal stress by exposing pupae to a constant temperature, ranging from stressfully cold to stressfully hot (10 ◦C, 26 ◦C, 28 ◦C, 32 ◦C), for three days. Offspring …

Ai-Enhanced Fluorescein Angiography Detection Of Diabetes-Induced Silent Retinal Capillary Dropout And Rna-Seq Identification Of Pre-Symptomatic Biomarkers, Yiyan Peng, Huishi Toh, Dennis Clegg, Peng Jiiang

Biological, Geological, and Environmental Faculty Publications

Objective: Retinal capillary dropout, characterized by acellular capillaries or "ghost vessels," is an early pathological sign of diabetic retinopathy (DR) that remains undetectable through standard clinical imaging techniques until visible morphological changes, such as microaneurysms or hemorrhages, occur. This study aims to develop a non-destructive artificial intelligence (AI)-based method using fluorescein angiography (FA) images to detect early-stage, silent retinal capillary dropout.

Methods: We utilized 94 FA images and corresponding destructive retinal capillary density measurements obtained through retinal trypsin digestion from 51 Nile rats. Early capillary dropout was defined as having an acellular capillary density of >= 18 counts per mm2. …

Phenological Plasticity Provides Limited Resilience To Climate Warming In A Sea Turtle Species With Temperature-Dependent Sex Determination, Blair P. Bentley, Lisa M. Komoroske, Cintia M. Santos, Armando J. B. Santos, Estefany Argueta, Vic Quennessen, Christina M. Coppenrath, Camille Kynoch, Vincent S. Saba, Claudio Bellini, Rafaely N. M. S. Ventura, J. Wilson White, Mariana M. P. B. Fuentes

Biological Sciences: Faculty Publications

Anthropogenic climate change is threatening global biodiversity, with sea turtles particularly vulnerable as offspring sex and developmental success are strongly influenced by incubation temperature. Behavioral plasticity, including the seasonal distribution of reproductive output, may provide short-term mechanisms for mitigating these impacts. Here, we investigated season-wide hatchling sex ratios and emergence success in a small population of green turtles (Chelonia mydas), tracking individual females across their nesting seasons. Sex ratios varied markedly through the nesting season, with later nests producing a greater proportion of male hatchlings. Moreover, sex ratios were relatively consistent among nests laid by individual females. Overall, females producing …

Asm And A Culture Of Sharing, Caring, And Fun In Education, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

One doesn't always equate a professional scientific society with sharing, caring, and fun, but in this age of public distrust, public engagement with science and active student learning are ways to regain trust. ASM holds a leadership position in promoting and recognizing the value of education in the future of our profession and is there to support your educational journey. From K12 outreach and public engagement, to professional development, to presenting and publishing scholarship of teaching and learning, ASM has been at the forefront of biology education. Under the leadership of visionary directors such as Amy Chang and now Irene …

Attraction Of Nocturnal Scarab Beetles By Unusual Floral Volatiles In A Banksia (Proteaceae) With Functionally Diverse Pollinators, S. K. Wawrzyczek, B. Bohman, S. L. Krauss, I. M. Butler, G. R. Flematti, K. Farnier, S. E. Hoebee, R. A. Davis, R. D. Phillips

Research outputs 2022 to 2026

Pollination by beetles has evolved multiple times in flowering plants but with relatively few plant species adapted specifically to pollination by scarab beetles (Coleoptera: Scarabaeidae). However, some plant species may produce floral scents and offer floral rewards that attract scarabs alongside other pollinator functional groups. In Banksia attenuata (Proteaceae), nocturnal observations led to the discovery of extensive floral visitation by beetles, alongside birds, mammals and diurnal insects. Given the distinctive melon-like floral scent of B. attenuata, we predicted long-distance attraction of beetles by chemical cues. We undertook floral visitor surveys aided by camera traps and video recordings, and quantified insect …

Land Condition Standards For The Western Australian Nullarbor Pastoral Region, Peter-Jon A. Waddell, Wayne J. Fletcher

Natural resources published reports

This report outlines the quantitative land condition standards that define acceptable pastoral land condition for the Western Australian part of the Nullarbor, to meet the regulatory requirements of the Land Administration Act 1997 and the Soil and Land Conservation Act 1945.

The Department of Primary Industries and Regional Development is developing regional-level pastoral land condition standards for Western Australia as a critical component of the Framework for sustainable pastoral management – revised edition (Fletcher 2022), the purpose of which is to address the Office of the Auditor General’s recommendations to improve the management of pastoral lands in WA …

Alfred Russel Wallace Notes 42: How Accurate Are The Transcriptions Presented At The Alfred Russel Wallace Page Website?, Charles H. Smith

Faculty/Staff Personal Papers

A look is taken at the level of accuracy displayed by the transcriptions of Wallace writings offered at the Alfred Russel Wallace Page website, as determined by a ChatGPT analysis.

The Massbank Contributions Of The Mfam Collaboration, Anusha Ahlendorf, Asaph Aharoni, Khabat Vahabi, Tillmann G. Fischer, Pierre Marie Allard, Megan Augustin, Ulschan Bathe, David Broadhurst, Corey Broeckling, Joerg Buescher, Adrian Covaci, Katyeny Manuela Da Silva, Ric C.H. De Vos, Micha Gracianna Devi, Stefanie Döll, Maximilian Frey, Andrej Frolov, Emmanuel Gaquerel, Vasuk Gautam, Esteban Charria-Girón, Alain Goossens, Jeremy Grosjean, Maria Halabalaki, Elias Iturrospe, Kim Kultima, Stephanie Herman, Toni M. Kutchan, Romain Larbat, René Meier, Eleni V. Mikropoulou, Gregory Mouille, Luca Nicolotti, Nir Shahaf, François Perreau, Pierre Pétriacq, Michael Reichelt, Stacey N. Reinke, Rani Robeyns, Alena Soboleva, Otmar Spring, Akshai Parakkal Sreenivasan, Alain Tissier, Jean Chrisologue Totozafy, Hiroshi Tsugawa, Josep Valls-Fonayet, Maria Van De Lavoir, Justin J.J. Van Der Hooft, Fredd Vergara, David Wishart, Ludger A. Wessjohann, Jean-Luc Wolfender, Jörg Ziegler, Gerd Ulrich Balcke, Steffen Neumann

Research outputs 2022 to 2026

Introduction: The analysis of metabolic profiles using high resolution mass spectrometry (MS) data provides deep insights into biological processes. In metabolomics, MS analysis generates a large number of features that represent metabolites. However, identifying specific metabolites from these features can be challenging. One of the major bottlenecks in the metabolomics field is the identification of MS features, which is a prerequisite for any biochemical interpretation. By identifying similarities and differences within a metabolite family (mFam), evaluating MS features at the metabolite family level can help assigning functional roles to individual MS features. These data can help interpreting metabolic pathways and …

Nh Dfl - 25 Years Of Hwa - Peterborough_2026_Aug_11, Ryan Crandall

UNH Cooperative Extension

No abstract provided.

Thermoclock:A Novel Automated Temperature Regulation Device That Can Model Circadian Entrainment And Disruption In 2d And 3d In Vitro Models, Kaiyin Kelly Zhang, Cathryn A. Cutia, Chanelle A. Moise, Bhavna Kalyanaraman, Kaitlyn Chhe, Jiexin Wang, Michelle E. Farkas, Ilia N. Karatsoreos, Mary Harrington, Meghan E. Huber, Cathal J. Kearney

Biological Sciences: Faculty Publications

Circadian rhythms are critical for maintaining homeostasis and regulating physiological functions, and consequentially impact disease progression; yet, they remain largely overlooked in in vitro models used in preclinical research. One major barrier to rigorously testing the role of circadian rhythms in these models is the lack of accessible tools that seamlessly integrate into standard culture setups and are capable of sustainably delivering time cues to cells and tissues in long term experiments. Here, we present the ThermoClock, a low-cost, Arduino-based automated temperature control system capable of delivering independent temperature programs to multiple cultures simultaneously. Using circadian reporter U2OS cell lines …