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Articles 2161 - 2190 of 39062
Full-Text Articles in Biology
Effects Of Predation On Cognition In Brachyrhaphis Rhabdophora, Elizabeth F. Jenkins, Jerald B. Johnson
Effects Of Predation On Cognition In Brachyrhaphis Rhabdophora, Elizabeth F. Jenkins, Jerald B. Johnson
Library/Life Sciences Undergraduate Poster Competition 2025
Does predation environment affect the cognitive abilities of the tropical fish Brachyrhaphis rhabdophora?
Barks Vs Bears: A Ruff Reality, Fern Luttrell, Tom Smith, Stephen Herrero, Lana Ciamiello, Hank Hristienko, Erin Jacoway, Linda Wiggins
Barks Vs Bears: A Ruff Reality, Fern Luttrell, Tom Smith, Stephen Herrero, Lana Ciamiello, Hank Hristienko, Erin Jacoway, Linda Wiggins
Library/Life Sciences Undergraduate Poster Competition 2025
- Will your dog protect you from a bear?
- Do dogs provoke bear attacks?
- Is it safe for your dog to be in bear country?
Assessing Sandy Beach Health Throughout The South Atlantic Bight With Ghost Crabs, Raquel Wilson, Blaine D. Griffen
Assessing Sandy Beach Health Throughout The South Atlantic Bight With Ghost Crabs, Raquel Wilson, Blaine D. Griffen
Library/Life Sciences Undergraduate Poster Competition 2025
To better inform the management and protection of marine ecosystems, we examined human impacts on sandy beach health across the South Atlantic Bight by assessing the abundance and size of ghost crab burrows, and habitat quality. We used an urbanization index to measure the level of human impact. Sandy beach ecosystems are ecologically and economically important, yet they face immense pressure from increasing human activity1,2. Protecting these ecosystems is critical yet challenging due to their large size, but we can evaluate their health and the extent of human impact using a bioindicator species. We used the Atlantic ghost …
Those Dam Fish: Morphological Response In Two Riverine Fish Species Following Dam Construction, Leah Stimpson, Austin Rejcek, Mark C. Belk
Those Dam Fish: Morphological Response In Two Riverine Fish Species Following Dam Construction, Leah Stimpson, Austin Rejcek, Mark C. Belk
Library/Life Sciences Undergraduate Poster Competition 2025
Flow regime is a known predictor of fish shape1. Conspecific populations living in distinct flow environments often exhibit different morphologies.
Dams present a unique opportunity to observe a dramatically transformed flow regime. Fish populations that once occupied a fast-flowing habitat suddenly live in an artificial reservoir.
The Parana Basin in southern Brazil boasts some of the greatest fish diversity in the world. The biological implications of hydroelectric dam construction along its major rivers must be studied.
Previous literature has been limited to a single species or ecological niche2.
➢ Our objective was to describe what morphological …
I’M Just Like You: Do Sympatric Lizard Species Exhibit Convergent Morphology? (Genus Sceloporus), Abigail Lamartine, Alison Whiting
I’M Just Like You: Do Sympatric Lizard Species Exhibit Convergent Morphology? (Genus Sceloporus), Abigail Lamartine, Alison Whiting
Library/Life Sciences Undergraduate Poster Competition 2025
The spiny lizards, genus Sceloporus, are the most speciose genus of reptiles endemic to North America. These lizards are commonly seen doing “push-ups” and head bobs, showing off their bright blue belly and throat patches (figure 5).Sceloporus use these displays to attract mates and defend their territory, and the size and color of patches is the most commonly used character to identify species(see figure 3). Within southwestern Utah, there is a small region where two species, S.occidentalis and S. tristichus, overlap in range (figure 1), and lizards can be found that appear to have patches intermediate between the two …
Frog Barcodes: Scanning The Secret Species Of Pelodryadinae, Kennedy Hunter, Taylor Probst, Alison Whiting
Frog Barcodes: Scanning The Secret Species Of Pelodryadinae, Kennedy Hunter, Taylor Probst, Alison Whiting
Library/Life Sciences Undergraduate Poster Competition 2025
Tree frogs (Hylidae) have unique adaptations such as toe pads for life in the trees (figure 1).
The subfamily Pelodryadinae is poorly known and primarily found in the Australopapuan region.
Papua New Guinea is the island with the greatest frog diversity but many species remain undescribed.
DNA barcoding has been shown to be an effective tool in species delimitation and for examining species diversity in poorly understood groups.
Epigenetic Reprogramming In Pediatric Tumors Predicts Active Pathways In The Tumor Microenvironments, Noah Moffat, Clark Tolbert, Colin Wessman, Matthew H. Bailey
Epigenetic Reprogramming In Pediatric Tumors Predicts Active Pathways In The Tumor Microenvironments, Noah Moffat, Clark Tolbert, Colin Wessman, Matthew H. Bailey
Library/Life Sciences Undergraduate Poster Competition 2025
Figure 1: We collected samples from five different studies that analyzed and generated spatial transcriptomic data from adult and pediatric brain tumors1-4.
Understanding The Individual Behavioral Variation Of Asian Elephants (Elephas Maximus) As An Indicator Of Human Elephant Conflict, Julia Brzezicki
Understanding The Individual Behavioral Variation Of Asian Elephants (Elephas Maximus) As An Indicator Of Human Elephant Conflict, Julia Brzezicki
Masters Theses & Specialist Projects
Human-Elephant Conflict (HEC) is an increasingly common event that arises from resource competition due to overlaps in human and elephant environments. Using a conservation behavior approach, the behaviors of elephants can be studied to enhance conservation efforts to mitigate HEC by better predicting which elephants engage in HEC. To understand the factors that affect individual behavioral types of adult Asian elephants (Elephas maximus), my project examined the behavioral patterns displayed by an elephant population in Sri Lanka. I collected demographic and behavioral data of a subset of 15 adult elephants to determine individual differences in five behavioral categories: Affiliation, aggression, …
A Systematic Study Of The Highly Endemic Spider Beetle Fauna (Coleoptera: Ptinidae: Ptininae) Found In The Coastal Deserts Of Perú, Marlena Askren
A Systematic Study Of The Highly Endemic Spider Beetle Fauna (Coleoptera: Ptinidae: Ptininae) Found In The Coastal Deserts Of Perú, Marlena Askren
Masters Theses & Specialist Projects
Spider beetles are small, often flightless insects that occur in hyperarid environments around the world. They exhibit interesting morphology and behavior that allows them to conserve water they obtain from plant material, dung, and other detritus. One hyperarid region in which they are found is the Pacific coast of Perú and northern Chile. The Sechura Desert, Peruvian Coastal Desert, and Atacama Desert make up the arid coast where spider beetles occur from north to south. Through multiple years of field work and museum collections, populations of spider beetles were found along the coast in isolated pockets of vegetation, separated by …
Biocultural Mobility: The Impact Of Osteoarthritis On Rural Kentuckians, Alexandria Anderson
Biocultural Mobility: The Impact Of Osteoarthritis On Rural Kentuckians, Alexandria Anderson
Mahurin Honors College Capstone Experience/Thesis Projects
Osteoarthritis (OA) is a common chronic degenerative joint disease that results in joint pain and loss of joint function, and it is rising in prevalence alongside an aging population. OA has a complex etiology, with various biological, mechanical, and sociocultural factors affecting its acquisition and progression. Like most diseases, OA has a differential prevalence among populations; it is disproportionally more common in underserved communities, including rural areas. This thesis presents (1) a study comparing selected OA treatment type and patient travel time, (2) a study comparing OA comorbidity amount and rurality, and (3) a discussion of exploratory interdisciplinary methods in …
Characterization Of Legionella Pneumophila Effector Protein Ceg10, Emmanuella Ofoka
Characterization Of Legionella Pneumophila Effector Protein Ceg10, Emmanuella Ofoka
Masters Theses & Specialist Projects
Legionella pneumophila, the causative agent of Legionnaires' disease, utilizes a Type IV secretion system (T4SS) to transport effector proteins into host cells. This bacterium is said to have over 300 effector proteins. Among these, the Ceg10 effector protein had remained uncharacterized, despite evidence suggesting the importance of bacterial effector proteins in the host-pathogen interaction. This study aimed to perform a structural and functional characterization of Ceg10 through transcriptomic, bioinformatics, and molecular analyses. Transcriptomic profiling of HEK 293T cells expressing Ceg10 revealed significant differential gene expression, with approximately 20 upregulated genes, including leucine-rich repeat, as well as other downregulated genes. …
Learning In Nonleafcutting Fungus-Gardening Ants, Elizabeth D. Boshers
Learning In Nonleafcutting Fungus-Gardening Ants, Elizabeth D. Boshers
Biology Theses
Social complexity influences learning and other cognitive behaviors, but for insects, this influence is not well understood. Delayed rejection is an aversive learning behavior previously studied exclusively in leafcutting ant species, the most socially complex of the fungus-gardening ants. Fungus-gardening ants are unique in that they do not directly consume the material they forage, using it instead as substrate to cultivate a symbiotic fungus on which the ants are obligately dependent. Here, I attempt to induce delayed rejection in several fungus-gardening ant species that exhibit a range of social complexity, then compare the characteristics of delayed rejection behavior among species. …
Acoustic Monitoring Of Oak Toads (Anaxyrus Quercicus), A Longleaf Pine (Pinus Palustris) Ecosystem Endemic Amphibian, Alex Ward Foote
Acoustic Monitoring Of Oak Toads (Anaxyrus Quercicus), A Longleaf Pine (Pinus Palustris) Ecosystem Endemic Amphibian, Alex Ward Foote
Theses, Dissertations and Capstones
The Longleaf pine ecosystem is one of the most endangered ecosystems in the world with only 3% remaining. Many endemic species to the longleaf pine ecosystem are also in decline and suffer from habitat loss resulting from fire exclusion, urbanization, agriculture, and industrial silviculture. Oak Toads (Anaxyrus quercicus) are a longleaf pine ecosystem obligate; the species is experiencing declines across its range and only has protections in Virginia and North Carolina. We used data from an array of Automated Recoding Units across four years (2013, 2014, 2015, 2019) at three state-owned wildlife management areas to capture audio files …
A Metadata Analysis Of Unionid Mussels Of The Greenup Pool Of The Ohio River Using Occupancy Modeling And Species Area Curves, Tyler Mitchell Annarino
A Metadata Analysis Of Unionid Mussels Of The Greenup Pool Of The Ohio River Using Occupancy Modeling And Species Area Curves, Tyler Mitchell Annarino
Theses, Dissertations and Capstones
Although North America contains the largest diversity of freshwater mussels in the world, only ~24% of species in the United States and Canada have stable populations. This group provides unique hurdles for surveying, and current unionid survey protocols include neither longitudinal studies nor randomization of sites. This project conducts a metadata analysis of freshwater mussels in the Greenup Pool of the Ohio River (GPOR) to answer three questions: 1. Does a combination of longitudinal scope, randomization of sites, and historic methodology of surveying result in useful occupancy/detection models (ODMs) of unionids in large streams at the family level? 2. Does …
Propelling Whole-Cell Biosensor Creation By Leveraging Gene Fusion To Assemble Synthetic Functional Transcription Factors For The Detection Of Biomarkers, Samuel J. Verzino
Propelling Whole-Cell Biosensor Creation By Leveraging Gene Fusion To Assemble Synthetic Functional Transcription Factors For The Detection Of Biomarkers, Samuel J. Verzino
Theses and Dissertations
Biosensors are analytical devices that leverage biological modules for the detection of molecules of interest. As customizable tools designed to aid in a wide array of diagnostics, these instruments are rapidly advancing the field of synthetic biology. While varied in their construction and purpose, biosensors are ultimately concerned with converting a physicochemical stimulus into a detectable signal. Among the many existing variants, whole-cell biosensors (WCBs) stand out due to their versatility and engineering potential. WCBs are a type of biosensor in which a physiologically active cell sustains an array of biomolecular elements - receptors, transporters, regulators, etc. - capable of …
Analysis Of The Influence Of Telomere Length Variation On Flowering Time And Plant Fitness In Arabidopsis, Brianna Spadavecchia
Analysis Of The Influence Of Telomere Length Variation On Flowering Time And Plant Fitness In Arabidopsis, Brianna Spadavecchia
Theses, Dissertations and Capstones
Telomeres are conserved structures at the ends of eukaryotic chromosomes that promote genome stability. In most eukaryotes, the DNA found within these protective end caps consists of short, G-rich nucleotide repeats. Cells with critically short telomeres face a variety of potential adverse outcomes such as end-to-end chromosome fusion, apoptosis, etc. The length of telomeric DNA varies substantially between different plant species and even between genotypes of the same species. A previous study using T-DNA mutants of the model plant Arabidopsis thaliana with long or short telomeres suggested that telomere length may be an adaptive trait. Specifically, for reproductive fitness, the …
Climate And Gypsum Parent Material Shape Biocrust Communities And Moss Ecology In The Chihuahuan And Mojave Deserts, Katelyn Gobbie, Nicole Pietrasiak, Brian M. Jusko, Rebecca E. Drenovsky
Climate And Gypsum Parent Material Shape Biocrust Communities And Moss Ecology In The Chihuahuan And Mojave Deserts, Katelyn Gobbie, Nicole Pietrasiak, Brian M. Jusko, Rebecca E. Drenovsky
2025 Faculty Bibliography
Biological soil crust communities (biocrusts) establishing on gypsum soils have been well-documented for their prolific appearance and rich diversity of lichens and bryophytes. However, studies characterizing gypsum biocrusts have occurred primarily outside of the U.S., most of which lack comparisons to other soil types. We conducted intensive field surveys to evaluate the ground cover and frequency of biocrust functional groups and moss species on gypsum and non-gypsum soils in the U.S. regions with the most extensive gypsum outcrops, the northern Chihuahuan and eastern Mojave Deserts. Study sites were stratified by geomorphology and paired, so that every gypsum site was matched …
Friend And Foe: Tgf-Beta, Its Role In Tumor Development, And An Analysis Of Chung Et Al. (2018), Yosef Sonbolian
Friend And Foe: Tgf-Beta, Its Role In Tumor Development, And An Analysis Of Chung Et Al. (2018), Yosef Sonbolian
The Science Journal of the Lander College of Arts and Sciences
This paper will analyze an important study performed by Chung et al. (2018) regarding transforming growth factor-beta (TGFbeta), which plays an important role in various cellular processes. Paradoxically, TGF-beta plays a different role in the development of cancer at various points. In early-stage tumors, TGF-beta has been known to inhibit cancer growth, but in later stages, it acts as a cancer promoter. Chung et al. (2018) attempted to ascertain the efficaciousness of the drug Sorafenib in suppressing TGF-beta responses, as well as the ways that this drug achieves its goal and the implications that their findings may have for future …
Validation Of Non-Invasive Methods For The Measurement Of Gonadal And Inter-Renal Steroid Hormones In A Desert-Adapted Amphibian (Scaphiopus Couchii), Alexander T. Baugh, Callie Cho , '23, Alice Onyango-Opiyo , '23, Sophie A. Rodner , '25, Senna Mieth , '25, Daniel Oakes , '24, Liam Halstead , '25
Validation Of Non-Invasive Methods For The Measurement Of Gonadal And Inter-Renal Steroid Hormones In A Desert-Adapted Amphibian (Scaphiopus Couchii), Alexander T. Baugh, Callie Cho , '23, Alice Onyango-Opiyo , '23, Sophie A. Rodner , '25, Senna Mieth , '25, Daniel Oakes , '24, Liam Halstead , '25
Biology Faculty Works
For aquatic and semi-aquatic vertebrates like amphibians, it is possible to estimate excreted hormone levels using non-invasive methods such as waterborne and salivary sampling. These techniques allow monitoring of endocrine activity over varying, repeated and simultaneous integration periods while minimizing handling-related stress that can ‘contaminate’ hormone estimates, including estimates of baseline glucocorticoids. Here we have validated the extraction and quantification of three steroid hormones (corticosterone, CORT; 17-b estradiol, E₂; testosterone, TST) in Couch’s spadefoots (Scaphiopus couchii)—a desert-adapted anuran of special interest for physiology, evolution and conservation—using non-invasive waterborne and minimally invasive salivary hormone methods. We combined extraction and …
Noncoding Rnas In Nuclear Organization, Thembalami Dube , '24, Dawn M. Carone
Noncoding Rnas In Nuclear Organization, Thembalami Dube , '24, Dawn M. Carone
Biology Faculty Works
No abstract provided.
Recruitment, Reprogramming, And Function Of Trpv1+ Sensory Nerves In The Development And Progression Of Head And Neck Cancer, Anthony Restaino
Recruitment, Reprogramming, And Function Of Trpv1+ Sensory Nerves In The Development And Progression Of Head And Neck Cancer, Anthony Restaino
Dissertations and Theses
Head and neck squamous cell carcinomas (HNSCC) are an epithelial cancer prevalent across the world and are divided into two subcategories: human papillomavirus-mediated (HPV+) and carcinogen-mediated (HPV-) HNSCC. These subgroups have distinct patient populations, divergent responses to therapy, and prognoses, however the foundational mechanisms that facilitate these differences are not well understood or defined. Recently, the presence and function of sensory neurons within the tumor environment of various solid tumors has been identified and investigated. Herein, we identify differences in the presence of TRPV1+ sensory neurons and neuron structures in the tumor environment of HPV+ and HPV- HNSCC, correlating with …
What Are The Genetic Influences Of Alcohol Sensitivity And Alcohol Metabolism? A Narrative Review Of Human Studies, Erwin Murray, Karen G. Chartier Phd, Msw
What Are The Genetic Influences Of Alcohol Sensitivity And Alcohol Metabolism? A Narrative Review Of Human Studies, Erwin Murray, Karen G. Chartier Phd, Msw
UROP Posters
Background: Alcohol sensitivity and alcohol metabolism are two phenotypes associated with alcohol use disorder (AUD) influenced by genetics. This narrative review aims to answer “What are the genetic influences of alcohol sensitivity and alcohol metabolism?” to gain a better understanding of the genetic aspects of AUD.
Methods: A database search was performed in PubMed. Empirical human genetic studies published between 2009 and 2025 that focused on alcohol sensitivity (measured by the Self-Rating of the Effects of Alcohol scale) or alcohol-metabolizing genes were eligible for review. Studies were screened to determine inclusion for the current review.
Findings: Fifteen …
Light, Symbiosis, And The Biology Of Corals, Taylor R. Lindsay
Light, Symbiosis, And The Biology Of Corals, Taylor R. Lindsay
Open Access Dissertations
Light is critical for the survival of most shallow water scleractinian corals. Corals are mixotrophs, acquiring energy via both feeding on water column plankton and photosynthesis via a symbiotic relationship with dinoflagellates living within their tissues. Corals rely heavily on photosynthetically derived metabolites through this symbiotic relationship, intrinsically linking their biology to light availability. Corals are known to modulate their biology to maximize light capture. However, there is a limited understanding of the interaction between morphology, physiology, and energetic strategy, as well as how light may affect these relationships. In Chapter 1, we quantified the morphology, physiology and energetic …
Impacts Of Multiple Coastal Stressors Across Life-History Stages In The Eastern Oyster, Amaelia Zyck
Impacts Of Multiple Coastal Stressors Across Life-History Stages In The Eastern Oyster, Amaelia Zyck
Open Access Dissertations
Urbanized estuaries are characterized as a complex of biotic and abiotic stressors, which currently challenge marine life and are expected to intensify and become increasingly unpredictable under the ongoing impacts of climate change. The persistence of coastal species that inhabit these stressful environments will ultimately depend on their ability to adapt. Many of these species have complex life cycles, featuring distinct morphological and physiological developmental stages that can exhibit unique responses to environmental pressures. However, since all stages share the same genome, selective pressures acting on one stage can have cascading effects throughout the life cycle. The larval stage, being …
Non-Coding Rnas As Regulators Of Coral Biology, Jill Ashey
Non-Coding Rnas As Regulators Of Coral Biology, Jill Ashey
Open Access Dissertations
Coral reef ecosystems face increasing threats from climate change, yet knowledge of the molecular mechanisms that underlie coral acclimatization, resilience, and development remains incomplete. Among these mechanisms, non-coding RNAs (ncRNAs), specifically microRNAs (miRNAs), are emerging as key regulators of gene expression in response to environmental and developmental cues. This dissertation explores the diversity, function, and dynamics of ncRNAs in scleractinian corals across ecological, physiological, and developmental contexts. In Chapter 1, I present the first comprehensive analysis of ncRNAs - specifically long ncRNAs (lncRNAs), miRNAs, and piwi-RNAs (piRNAs) - in three reef-building coral species from Mo’orea, French Polynesia: Acropora pulchra, …
Evaluating The Condition Of Age-0 Burbot In Relation To Diet, Cian Verbridge
Evaluating The Condition Of Age-0 Burbot In Relation To Diet, Cian Verbridge
Journal of Earth and Life Science
Burbot are becoming more and more popular for angling and aquaculture. However, populations have been declining across the world. Very little information is available about their early life and their interactions in the ecosystem due to challenges in sampling. Condition estimates and diet metrics were used to better understand the status of burbot in Lake Bemidji. Using backpack electrofishing, 45 young of the year burbot were collected. The lengths ranged from 86 to 146 mm and the wet weights ranged from 4.29 to 24.36 g. The stomach contents were removed and stored in ethanol for later analysis. The burbot were …
Modeling Wild Rice Bed Health: A Dual Metric Approach For Bed Strength And Invasive Impact, Seth Sisneros-Martinez
Modeling Wild Rice Bed Health: A Dual Metric Approach For Bed Strength And Invasive Impact, Seth Sisneros-Martinez
Journal of Earth and Life Science
Wild rice (mannoomin; Zizania palustris) is an aquatic grain that grows in slow-moving rivers and shallow bays throughout northern Minnesota. In Headquarters Bay on Leech Lake, wild rice has served as a cultural staple for Indigenous communities for centuries. However, its population has declined in recent years due to multiple environmental issues. One potential contributor is Eurasian watermilfoil (Myriophyllum spicatum; EWM), an invasive species that forms dense mats capable of outcompeting native aquatic vegetation, including wild rice. EWM was first recorded on the southern shoreline of Leech Lake in 2005 and has since become widespread. This study …
René Dubos: An Annotated Bibliography, Carol L. Moberg
René Dubos: An Annotated Bibliography, Carol L. Moberg
René Dubos: An Annotated Bibliography
René Dubos (1901–1982), a prominent 20th century scientist, humanist, and philosopher, was a prolific author. Affiliated for more than 50 years with The Rockefeller Institute for Medical Research/The Rockefeller University, his studies ranged from antibiotics and the role of bacteria in human diseases to formulating broad ecological and environmental questions affecting human health and disease. His seminal writings explore a lifelong belief that a living organism—whether microbe, human being, society, or the Earth itself—could be understood only through its relationships with everything else.
This comprehensive bibliography of more than a thousand works by René Dubos includes brief annotations for each …
The Effect Of Zebra Mussels On Minnesota Fish Communities, Benjamin D. Strei
The Effect Of Zebra Mussels On Minnesota Fish Communities, Benjamin D. Strei
Journal of Earth and Life Science
Zebra mussels Dreissena polymorpha are an aquatic invasive species that has been spreading quickly throughout Minnesota water bodies since 1989. Zebra mussels are known to change certain morphological conditions in lakes such as turbidity, phytoplankton abundance, and aquatic invertebrate populations. However, studies showing impacts of the infestation of zebra mussels on the health of a fish community have been hard to prove due to a lack of repeated surveys and a suite of other confounding variables such as natural and seasonal variability, plant community, and lake morphometry. Therefore, the objective of this study is to determine whether zebra mussels have …
Identification Of Benzothiophene-Derived Inhibitors Of Flaviviruses By Targeting Rna-Dependent Rna Polymerase, Leah Liu Wang, Shazeed-Ul Karim, Aidan Hand, Ryan Brunkhorst, Mackenna Petersen, Sarah Altam, Yi Liu, Luwen Zhang, Fengwei Bai, Shi-Hua Xiang
Identification Of Benzothiophene-Derived Inhibitors Of Flaviviruses By Targeting Rna-Dependent Rna Polymerase, Leah Liu Wang, Shazeed-Ul Karim, Aidan Hand, Ryan Brunkhorst, Mackenna Petersen, Sarah Altam, Yi Liu, Luwen Zhang, Fengwei Bai, Shi-Hua Xiang
School of Biological Sciences: Faculty Publications
Flaviviruses such as Dengue, West Nile, and Zika viruses are mosquito-borne RNA viruses that can cause serious diseases in humans. To develop effective drugs for treating these viruses’ infections, we create a new approach for developing common or shared drugs that may work for several different viral species of flaviviruses. It is based on the conserved RNA-dependent RNA polymerase (RdRp), which is the key enzyme for viral replication. We built up a common structure of RdRps (POLcon) from their consensus sequence. A conserved Triple-D structural motif was identified at the active site of POLcon that has been used for virtual …