Life Sciences Commons^™

Microplastic Analysis In Fish Larvae (Family: Gonostomatidae And Myctophidae) From The Banggai Sea, Indonesia, Noer Kholis, Luqman Ilyas Ardito, Luqmanul Hakim, Augy Syahailatua, Agus Saleh Atmadipoera, Rina Zuraida, Tuan Nurul Sabiqah Tuan Anuar, Mufti Petala Patria

Makara Journal of Science

Microplastic pollution is recognized as a threat to marine ecosystems, yet its impacts on early life stages of mesopelagic fishes remain poorly understood. This study investigated the occurrence and characteristics of microplastic ingestion in fish larvae of the families Myctophidae and Gonostomatidae from the Banggai Sea, Indonesia. Morphometric measurements and microplastic content were analyzed in 40 larvae (20 per family). Gonostomatidae larvae, despite being smaller in total length, exhibited significantly larger mouth widths and ingested more microplastic particles (mean = 3.90 ± 0.72 particles) than Myctophidae (mean = 3.40 ± 0.38 particles). Correlation analysis showed no significant relationships between morphometric …

In Silico And Toxicological Evaluation Of Crescentia Cujete Aqueous Extract: Potential Anticancer Activity Against Breast Cancer, Rahma Diyan Martha, Fatimah Fatimah, Syahputra Wibowo, Bantari Wisynu Kusuma Wardhani, Josephine Elizabeth Siregar, Elisabeth Farah Novita Coutrier, Alfia Fitrianita, Andri Pramesyanti Pramono, Ita Margaretha Nainggolan, Yudhi Nugraha, Danar Danar, Naufal Abiyyu, Lalu Unsunnidhal, Aswin Rafif Khairullah, Arif Nur Muhammad Ansori, Hesty Parbuntari, Wimbuh Tri Widodo

Makara Journal of Science

Majapahit plant (Crescentia cujete), commonly found in Indonesia, has not been extensively explored for its medicinal properties. Given that many cancer treatments are derived from plants, this study aimed to examine the effects of plants on cancer chemical composition of the C. cujete fruit pulp extract, identify its primary compounds, and assess its potential as an antioxidant, anticancer agent, particularly for breast cancer. An insilico approach was used to evaluate the toxicity and interactions, of the compounds with cancer biomarker proteins, in addition to an in vivo Brine Shrimp Lethality Test (BSLT) for BSLT, toxicity screening using six …

Morphometric And Genetic Analyses Of The Sheat Catfish Genus Ompok And Kryptopterus (Siluriidae) From The Mahakam River, East Kalimantan, Indone-Sia, Jusmaldi Jusmaldi, Rudhy Gustiano, Haryono Haryono, Rusdianto Rusdianto, Gema Wahyudewantoro, Syahroma Husni Nasution, Yulianto Yulianto, Muhammad Hunaina Fariduddin Aththar, Safar Dody, Amran Ronny Syam, Irin Iriana Kusmini, Yosmaniar Yosmaniar

Makara Journal of Science

Anthropogenic impacts within the watershed threaten the survival of sheat catfish. Therefore, species of the genera Ompok and Kryptopterus should be conserved and domesticated to ensure their long-term persistence. Moreover, the accurate documentation of the existence and taxonomic validity of Ompok and Kryptopterus species is crucial. Accordingly, this study aims to assess the species diversity of the genera Ompok and Kryptopterus in the Mahakam River, East Kalimantan, Indonesia. The study was conducted between June and December 2014, with an update in August 2024. Fish samples were collected from the upstream and middle reaches of the Mahakam River. A total of …

Screening For The Suitable Signal Peptide For Secreted Nattokinase Expression In E. Coli Using An In Silico Approach: Precursor Vs. Mature Forms, Dewi Purwani Caya Ningsih, Kartika Sari Dewi, I Wayan Martadi Santika, Ade Andriani, Joko Pebrianto Trinugroho, Muhammad Yusuf

Makara Journal of Science

Nattokinase (NK), an enzyme with thrombolytic activity, has been widely used in treating cardiovascular disease. The expression of NK in the E. coli cell host is favored due to its several benefits, though it often results in inclusion bodies. Appropriate signal peptides can be attached to the N-terminus of a protein to initiate a secretory expression system, thus preventing the nascent protein from aggregating in the cytoplasm. This study aimed to identify suitable signal peptides for the secretory expression of active NK in E. coli and to investigate the impact of pre-protein in NK secretion using an in silico approach. …

Ecological Patterns Of Culture-Recoverable Entomopathogenic Fungi From The Maize Rhizosphere Across Three Elevation Zones In West Java, Indonesia, Lilian Rizkie, Tjandra Anggraeni, I Nyoman Pugeg Aryantha, Agus Susanto

Makara Journal of Science

Entomopathogenic fungi (EPF) are important components of soil ecosystems and may support sustainable pest management in agricultural systems. This study characterized culture-recoverable EPF in maize (Zea mays L.) rhizospheric soils collected from 45 points across three elevation zones in West Java, Indonesia, using a spatially nested sampling design. Fungi were isolated by insect baiting with Tenebrio molitor larvae and direct soil plating. The isolates recovered were assigned to genera based on colony morphology and microscopic characteristics. Four EPF genera— Verticillium, Paecilomyces, Beauveria, and Metarhizium—were detected, with 34/45 samples (75.6%) positive for at least one recoverable …

House Sparrow Invasion Success Across Ecological, Immunological, And Molecular Scales, Kailey Marie Mccain

USF Tampa Graduate Theses and Dissertations

Biological invasions offer natural experiments for studying how organisms cope with ecological novelty. Although invasion success is often attributed to enemy release, introduced organisms do not enter enemy-free environments. Instead, they enter heterogeneous landscapes in which some familiar enemies are absent, others remain, and entirely novel enemies may be encountered. The question, then, is how invaders (hereafter non-natives) are poised to respond to these threats? This dissertation examines whether successful invasions in house sparrows (Passer domesticus) is associated with shifts in pathogen exposure, immune dynamics in blood, gut physiology, and gene co-expression network structure.

Chapter 1 is at the population/ecological …

Association Of Social Isolation, Loneliness, Social Strain And Support With Cancer Onset, And Frailty And All-Cause Mortality In People With Cancer, Joanne Elayoubi

USF Tampa Graduate Theses and Dissertations

Cancer is the second leading cause of death both in the United States and globally and for individuals over sixty-five. In 2026, approximately 2,114,850 new cancer cases are projected to emerge according to the National Center for Health Statistics (Siegel et al., 2026). Cancer incidence is increasing, making older adults sixty-five and older at risk of frailty and mortality following cancer. Although social isolation, loneliness, lack of social support, and social strain have been found to be risk factors for poor health and mortality, it is not clear the extent to which social relationships are associated with incident cancer, and …

Ptp4a2 Promotes Leukemogenesis Through Inhibiting The P53 Tumor Suppressor Signaling Pathway In Leukemia-Initiating Cells, Shiyu Xiao, Michihiro Kobayashi, Yunpeng Bai, Wenjie Cai, Sergio Barajas, Mohammed Abdullahel Amin, Sasidhar Vemula, Chonghua Yao, Yuxia Yang, Christopher Borchers

Faculty Work Comprehensive List

Acute myeloid leukemia (AML) is an aggressive hematological malignancy that is sustained by leukemia-initiating cells (LICs). Although protein tyrosine phosphatase 4A2 (PTP4A2), also known as phosphatase of regenerating liver 2 (PRL2), is highly expressed in AML, the mechanisms by which PTP4A2 promotes leukemogenesis are largely unexplored. In this study, we demonstrate that PTP4A2 promotes AML by inhibiting the p53 tumor suppressor pathway in LICs. Using KMT2A-MLLT3–driven AML as a model, we found that PTP4A2 deficiency activates p53 and induces LIC apoptosis and senescence, thereby extending the survival of recipient mice repopulated with Ptp4a2^−/− LICs. Mechanistically, PTP4A2 directly interacts with …

On The Edge Of Science?, Mark Briffa, Lynne U. Sneddon

Animal Sentience

In The Edge of Sentience, Jonathan Birch (2024) provides a thought-provoking synthesis of current perspectives on animal (and artificial) sentience. He addresses the question from conceptual, ethical, regulatory and empirical viewpoints. Here we focus on some of the scientific issues raised, including defining and operationalising the concept of sentience and the implications for policy and regulation. Despite unresolved conceptual challenges, the book makes a compelling case for the continued scientific investigation of animal sentience.

Regional, Functional And Transcriptomic Decoding Of Multidimensional Brain Structure Alterations In Obsessive-Compulsive Disorder, Leonardo Cardoso Saraiva, João Ricardo Sato, Isaac Sebenius, Nadza Dzinalija, Carla Del Río-Torné, Fábio Godinho, Antonio C. Lopes, Thomas V. Fernandez, Monicke O. Lima, Anderson M. Winkler

Human Genetics Publications

Studies of brain morphology in mental illness often focus on a few neuroimaging phenotypes. Here we present a comprehensive morphological characterization in obsessive-compulsive disorder (OCD) in a large sample (2255 OCD, 2264 controls) using nine cortical and four subcortical phenotypes, including several not previously examined in OCD, among them a subcortical structural similarity network phenotype developed here. Spatially distinct regional alterations emerged across structural phenotypes: cortical curvature alterations in default mode and frontoparietal networks, increased structural similarity network node degree in sensorimotor regions, widespread volume reductions associated with medication use, and localized subcortical shape alterations. In brain-behavior predictive models, curvature …

Closing The Gaps: Reforming Maryland Controls On Veterinary Antimicrobial Distribution, Samantha Capaldo, Kerry Mcclaughry

Journal of Food Law & Policy

Antimicrobials have been used in livestock and other food animals in the United States (“U.S.”) since the late 1940s. An antimicrobial is “[a]ny natural, synthetic, or semisynthetic substance that can eradicate or prevent the growth of microbes,” including “[a]ntibacterial, antifungal, antiparasitic, and antiviral medications.” Antimicrobial use in food animals is subject to a complex statutory and regulatory framework at both the state and federal levels. Antimicrobial use in food animals has been widely criticized for contributing to antimicrobial-resistant bacterial infections in humans. In response to this criticism, the U.S. Food and Drug Administration (“FDA”) and several states, including Maryland, have …

The Effect Of Ellagic Acid Isolated From Maclura Pomifera Fruit On The Staling Dynamics During The Storage Of Bread Products, Suvonqul S. Ravshanov, Anvar M. Normatov, Sarvinoz B. Nazarova, Sirojiddin Kh. Sadullayev, GuliraʼNo S. Bosimova

CHEMISTRY AND CHEMICAL ENGINEERING

This article investigates the effect of ellagic acid isolated from Maclura pomifera fruit on the staling dynamics during the storage of bread products. The study aimed to determine the effectiveness of ellagic acid's antioxidant and antimicrobial properties in preserving the microbiological stability and technological quality of bread. Experiments were conducted on bread samples with ellagic acid added at concentrations of 0.05% and other levels. The results showed a slowdown in the staling process, as indicated by moisture content, water retention, shrinkage, and penetrometric measurements. Specifically, at a concentration of 0.05%, ellagic acid helped to maintain the softness and elasticity of …

Humans Rule? Development Of Stewardship Mindsets Among Religious Undergraduates, Jamie Jensen, J Robert Nielson

ScholarsArchive Data

Two cohorts of students enrolled in a non-major introductory biology course were surveyed in 2020 and 2025 using the Revised New Ecological Paradigm (R-NEP) instrument. Open-ended student responses concerning their definition of ‘rule’ as a human prerogative were also analyzed using qualitative coding.

Comparison Of Alos-2 Palsar-2 L-Band Images For Fuel Moisture Discrimination In Forests In Mountainous Areas, Marin Loncar, Brigitte Leblon, Ashlin Richardson, Laura L. Bourgeau-Chavez, Dzhamal Amishev

Michigan Tech Publications

This study compares terrain-corrected polarimetric variables derived from L-band ALOS-2 PALSAR-2 acquired over a forested and mountainous region of southeast British Columbia (BC) during a drier and a wetter date. Backscatter, polarimetric decomposition parameters, and polarimetric discriminators were compared across 5 broad fuel types (forest, sparse, unforested, incomplete burn, complete burn) to examine if values differ between fuel moisture conditions as characterized by the value of the Drought Code (DC). Polarimetric variables suitable for use in fuel moisture modeling were identified using a one-way analysis of variance (ANOVA) test of significance applied to the normalized differences of these variables between …

Emergency Action Plan: Home, Family, Pets, And Livestock, Kristin Black, Michael Pate, Callahan K. Adams

All Current Publications

This document is an emergency family action plan designed to help your entire household prepare for emergencies. It aligns with the Youth Emergency Preparedness and Animal Project Safety Program available through Utah State University (USU). It can be taught by FFA teachers, 4-H leaders, Extension faculty/staff, and afterschool program educators. The Emergency Action Plan is intended for youth to bring home, lead their families in completing the sections, and guide them in preparing for and practicing emergency preparedness.

Promiscuous Mutualisms: John Dupré And The Centrality Of Symbiosis In The Living World, Thomas Pradeu

Presidential Fellows Articles and Research

My goal in this chapter is to show that symbiosis, especially symbiotic interactions between ‘macrobes’ (non-microbial organisms) and microbes, is a central topic in John Dupré’s work. This topic is undeniably one of the topics by which Dupré has gained wide recognition among practicing scientists, in addition to his recognition within the philosophical community. I spell out why symbiosis has turned out to be a sort of ideal trope for Dupré, as it encapsulates many of his main personal themes—from promiscuous realism to process ontology. I then show that immunology offers one interesting and complementary entry point into microbial symbiosis …

Effects Of Anchor Scheme On Torque And Neuromuscular Responses During And Following Fatiguing Isokinetic Forearm Flexion Tasks: Insights From High-Density Electromyography And Wavelet Analysis, Dolores Guadalupe Ortega

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This study examined the effects of anchor scheme on torque and neuromuscular responses during and following fatiguing isokinetic forearm flexion tasks. Thirteen men (mean ± SD; age: 22.0 ± 1.9 yr; height: 183.0 ± 6.4 cm; body mass: 90.2 ± 18.1 kg) completed pretest and posttest maximal, concentric, isokinetic forearm flexion muscle actions at 30°∙s-1 before and after continual, concentric, isokinetic forearm flexion tasks at 30°∙s-1 at an RPE of 6 (RPE6T) and the torque (TRQ6T) produced during the first repetition of the RPE6T. Monopolar electromyographic (EMG) signals were recorded from the biceps brachii using a high-density EMG sensor. A …

Lost In The Weeds No More! Revision Of The Robust Herb Gall Wasps (Hymenoptera, Cynipidae, Aulacideini) In North America, Louis F. Nastasi, Charles K. Davis, José Luis Nieves-Aldrey, Cecil N. Smith, Simon Van Noort, Ebony G. Murrell, Timothy S. Frey, Chris Friesen, M. J. Hatfield, Heather M. Hines, John F. Tooker, Andrew R. Deans

Department of Entomology: Faculty Publications

We revise the North American fauna of Aulacideini (Hymenoptera: Cynipidae) using morphological, ecological, and genetic (DNA barcode) data, incorporating observations from over 5,000 adult specimens and galls. Prior to revision, the fauna included 24 species in four genera. Six new genera and 25 new species are described, all authored by Nastasi: Argyrix gen. nov., Barathria gen. nov., Cosmita gen. nov., and Ketsia gen. nov., for species previously placed in Antistrophus Walsh; Prixima gen. nov., for a new species inducing galls on Pyrrhopappus DC.; and Tookeria gen. nov., for a new species inducing galls on Silphium L.; Antistrophus frieseni sp. nov., …

تأثير ارتفاع ثاني أكسید الكربون ، و الأشعة فوق البنفسجیة - ب، و ارتفاع درجة الحرارة على الكینوا, Mohamed Yousif

Thesis/ Dissertation Defenses

This is an experimental study that focuses on the present status of three factors that shape the current world global climate, and how it affects future agriculture, The study gives an insight on how effective are elevated CO₂, high UV-B irradiation and rising temperature on forage after 50 years’ time, keeping in mind the growing population and the increasing demand for proteins, it focuses on Chenopodium Quinoa, A C3 plant that is highly nutritious and known for its ability to tolerate high temperature, high salt concentration, and drought. Recently it got more focus and is cultivated in many places all …

Effect Of Abiotic Stress On Uae Native Acacia Plants: Salinity, Drought, Uv-B, Co₂, And Temperature Under Open Top Chamber (Otc), Fatima Nasser Alkaabi

Thesis/ Dissertation Defenses

The presence of abiotic stressors such as salinity, water shortage, hot weather, increase in atmospheric concentration of carbon dioxide and increase in UV-B radiations are major limiting factors to plant growth and survival, especially in desert and semi-desert conditions. These environmental limitations increase the exposure of the United Arab Emirates (UAE) to the lack of freshwater, extensive soil salinization, and rapid effects of climate change. As a result, the discovery of native plant species that can survive under various abiotic stress salinity and drought levels is the key to establishing the sustainable, ecologically adaptive landscaping and the successful ecological rehabilitation …

Advocating For A Care-Based Ethic: Stories Of Pregnancy And Birth From Caregivers Of Children With Down Syndrome, Lydia Ocasio-Stoutenburg, Nayma Sultana Mim

Developmental Disabilities Network Journal

Down syndrome (DS) impacts about 1 in every 640 childbirths in the United States. While quality of life has improved for people with DS, systemic inequities and deficit-based perceptions have persisted throughout history, with material impacts on people with DS and their caregivers. Researchers have documented how caregivers of children with DS have had negative experiences with healthcare providers and genetic counselors, whose biased views about DS translate into subtle and overt pressures. Few studies explicitly focus on the experiences of Caregivers of Color, multilingual families, as well as families with varying economic experiences who have children with DS. This …

Ion Channels As Targets For Parasiticides: A Brief Review Of Past, Present, And Future Directions, Josette V. Ricker, Mallory Mendenhall

Georgia Journal of Science

Scientists and healthcare providers have tried for decades to find parasite treatments that balance patient safety with efficacy in eliminating the pathogen. One approach is to target specifically the parasite’s important bodily functions while sparing the host. This brief review examines the historically significant antiparasitics - pyrethrins, picrotoxin, piperazine, and avermectins – all which specifically target parasite ion channels. We discuss the history of their discovery and use, which subsequently led to the development of new generations of parasiticides, and continues to inspire the search for safe and effective treatments.

Parental Effects In Fall Webworm, Emma Sellers, Mykaela Tanino-Springsteen, Mayra Vidal, Dhaval Vyas, Gina Wimp, Mariana Abarca, Mattheau Comerford, Shannon Murphy

Data

The environment or experiences of a parent generation can impact the fitness of the next generation, a phenomenon known as parental effects. While a high-quality diet can directly benefit an individual and a low-quality diet can reduce fitness, a parental effect occurs only when there is a transgenerational response to these stimuli that manifests in the individual’s offspring. In dietary generalists, a parental effect could play an important role for offspring performance if the species experiences considerable variation in quality across host plants; positive parental effects may result from host matching between parents and offspring on high-quality host plants, whereas …

Editorial: Plant Responses To Abiotic Stress: Unraveling Complex Mechanisms Through Genomics And Physiology, Mizanur Rahman, Takashi Asaeda, Md Harun Rashid

School of Earth, Environmental, & Marine Sciences Faculty Publications

No abstract provided.

Understanding The Needs And Practices Of Cut Flower Growers In Louisiana, Jessie M. Hoover

LSU Doctoral Dissertations

The specialty cut flower industry is expanding nationwide, including southeastern states such as Louisiana. Existing studies and market analyses focus on national trends of other regions with established floriculture networks, leaving a critical gap in understanding the unique challenges and opportunities faced by growers in Louisiana. This lack of data limits the ability of extension professionals, policymakers, and industry stakeholders to develop targeted strategies that support local production and market development. This study used qualitative case study methods to investigate the practices, challenges, and needs of 13 specialty cut flower growers in Louisiana. The study utilized Urie Bronfenbrenner’s (1979, 1986) …

Rna G-Quadruplexes Function As A Tunable Switch Of Fus Phase Separation, Jenny L. Carey, Miyuki Hayashi, Emily A. Welebob, Laura R. Ganser, Huan Wang, Kerry Buckhaults, Jacquelyn A. Depierro, Zheng Shi, James Shorter, Sua Myong, Aaron R. Haeusler, Lin Guo

Department of Biochemistry and Molecular Biology Faculty Papers

Fused in sarcoma (FUS) undergoes liquid-liquid phase separation (LLPS) to support essential cellular functions, but aberrant phase transitions promote toxic aggregation in neurodegenerative disease. Short RNA oligonucleotides can reverse this behavior, yet the structural determinants that govern RNA activity remain poorly defined. Here, we identify RNA G-quadruplexes (rG4s) as tunable structural motifs that potently modulate FUS LLPS. rG4 activity depends on its concentration and is modulated by rG4 length and stability: increasing repeat number switches rG4s from inhibitor to nucleator of FUS assembly, whereas chemical modifications that stabilize rG4 enhance inhibitory function and render these activities resilient to ionic perturbation. …

An Inf2-Dependent Actin-Mediated Step In Inositol 1,4,5-Trisphosphate Receptor Cluster Formation And Activity, Maite R. Zavala, Amrapali Ghosh, Suresh K. Joseph, Rajarshi Chakrabarti

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Intracellular calcium signaling plays a vital role in regulating various cellular processes including gene regulation, motility, metabolism, and cell death. Inositol 1,4,5-trisphosphate receptors (IP3Rs) on the endoplasmic reticulum (ER) are a major cation channel that regulates stimulus-induced calcium release from the ER. While several molecular players regulate the activity of IP3R, its regulation by actin filaments was uncharacterized. Here, we show that actin filaments polymerized by the specific actin nucleator INF2 facilitate agonist-induced IP3R activity. Our results demonstrate that INF2-mediated actin filaments regulate the formation and/or stability of IP3R clusters on the ER that have been previously shown to be …

Quantitative Genetic Analysis Of Baboon Facial Variation Challenges A Classic Papionin Trait, A. Thiebaut, C. C. Roseman, Michael Mahaney, Leslea J. Hlusko

Human Genetics Publications

The Linnaean family of monkeys that includes baboons, macaques, and mandrills (among others) is notably characterized by large, extended muzzles that vary in lateral nasal dorsum profile. In paleontological studies, this variation is referred to as the anteorbital drop (AOD). Here, we first devise a method to quantify this phylogenetic character. We then employ quantitative genetic analyses using a MCMCglmm model of facial variation in a captive, pedigreed baboon colony (n = 934), including admixed individuals derived from at least two Papio species, from the Southwest National Primate Research Center to assess the quantitative variation of the AOD, its …

Bovine Tuberculosis In The Middle East And North Africa: A Systematic Review And Meta-Analysis On Prevalence And Mycobacterium Bovis Clonal Complexes, Tsegaye Shamebo, Aboma Zewude, Temesgen Mohammed, Nabeeha Abdelgaleel Hassan, Mohammed Saleh Albreiki, Yassir Mohammed Eltahir, Atef Oreiby, Robert Barigye, Markos Tibbo, Ali Artaman, Gobena Ameni

All Works

Introduction – Bovine tuberculosis (bTB) is endemic in the Middle East and North Africa (MENA) region causing major threat to dairy production and milk consumers. This paper presents a comprehensive systematic review and meta-analysis of bTB prevalence and Mycobacterium bovis clonal complexes in the MENA region based on studies published between 2000 and 2025. Methods – A comprehensive literature search was conducted in PubMed, Scopus, Web of Science, Embase, and relevant regional databases. Thirty-eight studies met the criteria and included in the meta-analysis. Random-effects meta-analysis was used to estimate pooled prevalence. Additional analyses were done on sensitivity, meta-regression, and publication …

Butterfly Taxa Discovery Via Genomic Analysis, Jing Zhang, Qian Cong, Jinhui Shen, Leina Song, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

Expanding a genomics-guided assessment of butterfly taxonomy, we integrate phylogenetic evidence from all protein-coding genes with morphological considerations to refine our understanding of butterfly systematics and discover new taxa. As a result, 2 subgenera, 39 species, and 5 subspecies are proposed as new (type species in original combinations or type localities are listed in parentheses): Erebia (Magda) magdalena uintana Grishin, ssp. n. (USA: Utah, Uintah Co.) in Nymphalidae Rafinesque, 1815; Myselasia rondea Grishin, sp. n. (Brazil: Rondônia), Myselasia pseudocrinon Grishin, sp. n. (Ecuador: Pastaza), Myselasia paramatuta Grishin, sp. n. (Ecuador: Morona-Santiago), Euselasia anapellos Grishin, sp. n. (Peru: Loreto), …