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Production Of Single Cell Protein And Astaxanthin Using Methanol As Carbon Source, Corinne Pillarella, Craig Jahnke, Brielle Weiner 2019 University of Pennsylvania

Production Of Single Cell Protein And Astaxanthin Using Methanol As Carbon Source, Corinne Pillarella, Craig Jahnke, Brielle Weiner

Senior Design Reports (CBE)

Singlecell protein (SCP) is the biomass of unicellular organisms, such as bacteria or yeast, which is used commonly as a food source for animals. With a high protein content, a broad amino acid profile, and the ability to produce essential organic compounds and vitamins, SCP is a promising alternative to other classical sources of animal feed. Several processes have been developed to manufacture SCP for use in feedstocks for the sustainable farming of fish and other aquatic life, or aquaculture, which is one of the fastest growing food markets in the world. Here, a process is presented for the production ...


Culture-Derived Human Platelets For Clinical Transfusion, Alayna Choo, Gabrielle Leon, Susanna Jaramillo 2019 University of Pennsylvania

Culture-Derived Human Platelets For Clinical Transfusion, Alayna Choo, Gabrielle Leon, Susanna Jaramillo

Senior Design Reports (CBE)

Platelets are a vital component of human blood due to their role in clotting. Despite the incredible importance of platelets for human survival, there is very little supply of platelets to meet the clinical demand. Currently, platelets are only supplied from donations, whether through whole blood or apheresis. Therefore, there is a need for ex vivo means of producing platelets at a price that is competitive with the current market price. This project seeks to meet 65% of the demand for platelets in Philadelphia by producing platelets ex vivo in a process that utilizes shear stress to induce platelet formation ...


Ethanol To Ethyl Acetate, Kerri Heuser, Vinson Liao, Neha Narain 2019 University of Pennsylvania

Ethanol To Ethyl Acetate, Kerri Heuser, Vinson Liao, Neha Narain

Senior Design Reports (CBE)

Ethyl acetate is a specialty chemical with dominant use as a solvent, and total production exceeding 3.7MMT annually in 2017. Current production methods mainly utilize petrochemical-derived feedstocks of ethanol and acetic acid that undergo Fischer Esterification to produce ethyl acetate. This is traditionally considered to be an unsustainable process. A new startup company, Greenyug, based out of Santa Barbara, CA, has proposed a new design that solely utilizes fermentation ethanol, and, as a result, is considered sustainable. Greenyug describes (in accordance to US Patents US8562921B2, US9079851B2, and US9447018B2) a two-step reaction pathway that first partially dehydrogenates ethanol to acetaldehyde ...


Future Api Manufacturing Excellence, Emily Cunningham, Rachel Wilson, Marc Geagea 2019 University of Pennsylvania

Future Api Manufacturing Excellence, Emily Cunningham, Rachel Wilson, Marc Geagea

Senior Design Reports (CBE)

Many pharmaceutical manufacturing processes are costly, time-intensive, and energy-intensive. Due to the high operational costs, optimization of these processes would result in large economic savings. Fluid bed granulation takes inputs of air temperature, air flow rate, phase duration, binder spray rate, and inlet air humidity. A series of simulations were run to determine optimal operating conditions. It was determined that the process parameters should be limited to the following to meet product standards and reduce costs: air flow rate of 2800 (m3/h), inlet air temperature of 55˚C for spraying and 75˚C for drying, phase duration 124 ...


Process For Sustainably Sourced 2,5 Furan Dicarboxylic Acid, Michael Bell, David Gutierrez, Kevin Wang 2019 University of Pennsylvania

Process For Sustainably Sourced 2,5 Furan Dicarboxylic Acid, Michael Bell, David Gutierrez, Kevin Wang

Senior Design Reports (CBE)

Currently, there is a significant market for terephthalic acid (TPA)--a precursor for the ubiquitous plastic polymer polyethylene terephthalate (PET). As consumer preferences swing to more environmentally-friendly products, sustainable plastics, such as polyethylene furanoate (PEF), become more widely desired. In this project, the authors suggest a process for the synthesis of 2,5-Furandicarboxylic acid (FDCA), a diacid used in the synthesis of PEF, through a multi-step procedure following current industrial practices for TPA oxidation and patents from three sources: Synvina, a joint venture between Archer Daniels Midland (ADM) Company and DuPont Industrial Biosciences (DuPont), and Eastman. The first step of ...


Synthesis Of Green Hydrocarbons Using The Air To Fuels™ Technology, Tyler Durkin, Kyle Kersey, James Paolini 2019 University of Pennsylvania

Synthesis Of Green Hydrocarbons Using The Air To Fuels™ Technology, Tyler Durkin, Kyle Kersey, James Paolini

Senior Design Reports (CBE)

Our AIR TO FUELS™ project is based off a process designed by Carbon Engineering by the same name which seeks to synthesize liquid fuels from capture carbon dioxide captured from the atmosphere. The need for reduced greenhouse gases in our atmosphere is more urgent than ever and becoming more dire by the day. Still, our society’s reliance on liquid fuels like gasoline, diesel, and jet fuel is deeply rooted in our infrastructure and certainly not changing quickly or soon. The AIR TO FUELS™ process seeks to tackle both of these problems by taking carbon dioxide out of the atmosphere ...


Green Glycol: A Novel 2-Step Process, Ingemar Falcones, Sarah Golden, Maria Kowalchuk 2019 University of Pennsylvania

Green Glycol: A Novel 2-Step Process, Ingemar Falcones, Sarah Golden, Maria Kowalchuk

Senior Design Reports (CBE)

Ethylene glycol demand is growing rapidly, particularly in the global polyethylene terephthalate markets.¹ Traditional production of non-renewable ethylene glycol involves steam cracking of ethane or the methanol-to-olefin process to obtain ethylene.6 In response to environmental movements, Coca-Cola® began creating ethylene glycol from renewably sourced ethanol, by producing the ethylene oxide intermediate in a two-step reaction process.² Novel research at Leiden University, entitled Direct conversion of ethanol into ethylene oxide on gold based catalysts, explores a catalyst which produces ethylene oxide in one step, showing potential for a more efficient renewable process.³ This project explores the scaling ...


Understanding Conformational Regulation Of The Integrin I-Domain For Design Of Chimeric Protein Switches, Niall Phelan Terry, Liang Fang, J Vincent Price, Eric T. Boder Dr. 2019 University of Tennessee, Knoxville

Understanding Conformational Regulation Of The Integrin I-Domain For Design Of Chimeric Protein Switches, Niall Phelan Terry, Liang Fang, J Vincent Price, Eric T. Boder Dr.

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Within all complex biological processes intricate proteins are expressed to complete every niche and necessary task. Many express multiple allosterically regulated conformational states, with protein function regulated by effector molecules and other ligands. One such protein is the LFA-1 surface integrin protein and its inserted domain, the I-domain. We Isolated the I-domain for investigation of determining binding properties and understanding conformational regulations of affinity changes to its target ligand ICAM-1, for further use in chimeric protein switch design. A large change in binding affinity was found through the deletion of a sub-sequence of amino acids in I-domain known as the ...


Elucidating Growth Coupling Of Metabolites In E. Coli And Characterizing Modularity., Preston N. Nicely 2019 University of Tennessee, Knoxville

Elucidating Growth Coupling Of Metabolites In E. Coli And Characterizing Modularity., Preston N. Nicely

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

This project seeks to increase the efficiency of rapid engineering strain design by elucidating the functionality of a modular cell. The modular cell theory insinuates that there exists a baseline cellular framework from which cellular products can be produced by inserting a designed production module into the cell. In this study, recombinant gene cloning techniques were used to design metabolite production modules that focused on increasing the production, titer, and yield of ethanol and lactate within Escherichia coli (E. coli). An auxotrophic chassis cell was created by ModCell2 and multi-objective strain design to determine the genes necessary for deletion. The ...


Iron-Containing Nanoparticles For The Treatment Of Chrionic Biofilm Infections In Cystic Fibrosis, Leisha M. A. Martin 2019 University of New Mexico - Main Campus

Iron-Containing Nanoparticles For The Treatment Of Chrionic Biofilm Infections In Cystic Fibrosis, Leisha M. A. Martin

Nanoscience and Microsystems ETDs

Cystic fibrosis (CF) is the most common genetic disease resulting in the morbidity and mortality of Caucasian children and adults worldwide. Due to a genetic mutation resulting in malfunction of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein, CF patients produce highly viscous mucus in their respiratory tract. This leads to impairment of the mucociliary clearance of inhaled microbes. In addition to reduced microbial clearance, anoxic environmental conditions in the lungs promote biofilm-mode growth of the pathogenic bacterial species Pseudomonas aeruginosa. Chronic infections of P. aeruginosa begin in early childhood and typically persist until respiratory failure and death result. The ...


Design Of A Portable Biogas Purification And Storage System, Anikumar Kosna, Bade Shrestha 2019 Western Michigan University

Design Of A Portable Biogas Purification And Storage System, Anikumar Kosna, Bade Shrestha

Faculty Research and Creative Activities Award (FRACAA)

The objective of this study is to propose and design a commercial portable purification and storage system to commercialize biogas (which mainly consists of 45 - 75 % of methane and 15 - 16% of carbon dioxide), produced from household/non-commercial biogas plants. The selection of purification process is based on the portability of the purification system.


Amphiphilic Polyanhydride-Based Recombinant Muc4Β-Nanovaccine Activates Dendritic Cells, Kasturi Banerjee, Shailendra K. Gautam, Prakash Kshirsagar, Kathleen A. Ross, Gaelle Spagnol, Paul Sorgen, Michael J. Wannemeuhler, Balaji Narasimhan, Joyce C. Solheim, Sushil Kumar, Surinder K. Batra, Maneesh Jain 2019 University of Nebraska Medical Center

Amphiphilic Polyanhydride-Based Recombinant Muc4Β-Nanovaccine Activates Dendritic Cells, Kasturi Banerjee, Shailendra K. Gautam, Prakash Kshirsagar, Kathleen A. Ross, Gaelle Spagnol, Paul Sorgen, Michael J. Wannemeuhler, Balaji Narasimhan, Joyce C. Solheim, Sushil Kumar, Surinder K. Batra, Maneesh Jain

Chemical and Biological Engineering Publications

Mucin 4 (MUC4) is a high molecular weight glycoprotein that is differentially overexpressed in pancreatic cancer (PC), functionally contributes to disease progression, and correlates with poor survival. Further, due to its aberrant glycosylation and extensive splicing, MUC4 is a potential target for cancer immunotherapy. Our previous studies have demonstrated the utility of amphiphilic polyanhydride nanoparticles as a useful platform for the development of protein-based prophylactic and therapeutic vaccines. In the present study, we encapsulated purified recombinant human MUC4-beta (MUC4β) protein in polyanhydride (20:80 CPTEG:CPH) nanoparticles (MUC4β-nanovaccine) and evaluated its ability to activate dendritic cells and induce adaptive immunity ...


Stress Response To Co2 Deprivation By Arabidopsis Thaliana In Plant Cultures, Souvik Banerjee, Oskar Siemianowski, Meiling Liu, Kara R. Lind, Xinchun Tian, Dan Nettleton, Ludovico Cademartiri 2019 Iowa State University

Stress Response To Co2 Deprivation By Arabidopsis Thaliana In Plant Cultures, Souvik Banerjee, Oskar Siemianowski, Meiling Liu, Kara R. Lind, Xinchun Tian, Dan Nettleton, Ludovico Cademartiri

Statistics Publications

After being the standard plant propagation protocol for decades, cultures of Arabidopsis thaliana sealed with Parafilm remain common today out of practicality, habit, or necessity (as in co-cultures with microorganisms). Regardless of concerns over the aeration of these cultures, no investigation has explored the CO2 transport inside these cultures and its effect on the plants. Thereby, it was impossible to assess whether Parafilm-seals used today or in thousands of older papers in the literature constitute a treatment, and whether this treatment could potentially affect the study of other treatments.For the first time we report the CO2concentrations in Parafilm-sealed cultures ...


Streamlined Assessment Of Membrane Permeability And Its Application To Membrane Engineering Of Escherichia Coli For Octanoic Acid Tolerance, Miguel C. Santoscoy, Laura R. Jarboe 2019 Iowa State University

Streamlined Assessment Of Membrane Permeability And Its Application To Membrane Engineering Of Escherichia Coli For Octanoic Acid Tolerance, Miguel C. Santoscoy, Laura R. Jarboe

Chemical and Biological Engineering Publications

The economic viability of bio-production processes is often limited by damage to the microbial cell membrane and thus there is a demand for strategies to increase the robustness of the cell membrane. Damage to the microbial membrane is also a common mode of action by antibiotics. Membrane-impermeable DNA-binding dyes are often used to assess membrane integrity in conjunction with flow cytometry. We demonstrate that in situ assessment of the membrane permeability of E. coli to SYTOX Green is consistent with flow cytometry, with the benefit of lower experimental intensity, lower cost, and no need for a priori selection of sampling ...


Analyzing Drug Release Kinetics From Water-Soluble Polymers, Sean M. Kelly, Arun K. Upadhyay, Akash Mitra, Balaji Narasimhan 2019 Iowa State University

Analyzing Drug Release Kinetics From Water-Soluble Polymers, Sean M. Kelly, Arun K. Upadhyay, Akash Mitra, Balaji Narasimhan

Chemical and Biological Engineering Publications

The ability to develop predictive mathematical models of therapeutic release from pharmaceutical formulations has enormous potential to enhance our understanding of such systems and improve the controlled release of the payload. The current work describes the development and testing of a one-dimensional model of drug transport from amorphous, swelling/dissolving polymers. Model parameters such as the diffusivities of water and drug, the initial loading of the drug, the polymer dissolution rate, drug-polymer interactions, and the tablet thickness were varied, demonstrating the ability to tune the release to be controlled by either drug diffusion or polymer chain disentanglement. In addition, predictions ...


Degradation And Remodeling Of Epitaxially Grown Collagen Fibrils, Juan Wang, Anuraag Boddupalli, Joseph Koelbl, Dong Hyun Nam, Xin Ge, Kaitlin M. Bratlie, Ian C. Schneider 2019 Iowa State University

Degradation And Remodeling Of Epitaxially Grown Collagen Fibrils, Juan Wang, Anuraag Boddupalli, Joseph Koelbl, Dong Hyun Nam, Xin Ge, Kaitlin M. Bratlie, Ian C. Schneider

Materials Science and Engineering Publications

Introduction: The extracellular matrix (ECM) in the tumor microenvironment contains high densities of collagen that are highly aligned, resulting in directional migration called contact guidance that facilitates efficient migration out of the tumor. Cancer cells can remodel the ECM through traction force controlled by myosin contractility or proteolytic activity controlled by matrix metalloproteinase (MMP) activity, leading to either enhanced or diminished contact guidance.

Methods: Recently, we have leveraged the ability of mica to epitaxially grow aligned collagen fibrils in order to assess contact guidance. In this article, we probe the mechanisms of remodeling of aligned collagen fibrils on mica by ...


Computational Framework For The Identification Of Bioprivileged Molecules, Xiaowei Zhou, Zachary J. Brentzel, George A. Kraus, Peter L. Keeling, James A. Dumesic, Brent H. Shanks, Linda J. Broadbelt 2019 Northwestern University

Computational Framework For The Identification Of Bioprivileged Molecules, Xiaowei Zhou, Zachary J. Brentzel, George A. Kraus, Peter L. Keeling, James A. Dumesic, Brent H. Shanks, Linda J. Broadbelt

Chemistry Publications

Bioprivileged molecules are biology-derived chemical intermediates that can be efficiently converted to a diversity of chemical products including both novel molecules and drop-in replacements. Bridging chemical and biological catalysis by bioprivileged molecules provides a useful and flexible new paradigm for producing biobased chemicals. However, the discovery of bioprivileged molecules has been demonstrated to require extensive experimental effort over a long period of time. In this work, we developed a computational framework for identification of all possible C6HxOy molecules (29252) that can be honed down to a manageable number of candidate bioprivileged molecules based on analysis of structural ...


Enhancing The Co-Utilization Of Biomass-Derived Mixed Sugars By Yeasts, Meirong Gao, Deon Ploessl, Zengyi Shao 2019 Iowa State University

Enhancing The Co-Utilization Of Biomass-Derived Mixed Sugars By Yeasts, Meirong Gao, Deon Ploessl, Zengyi Shao

Chemical and Biological Engineering Publications

Plant biomass is a promising carbon source for producing value-added chemicals, including transportation biofuels, polymer precursors, and various additives. Most engineered microbial hosts and a select group of wild-type species can metabolize mixed sugars including oligosaccharides, hexoses, and pentoses that are hydrolyzed from plant biomass. However, most of these microorganisms consume glucose preferentially to non-glucose sugars through mechanisms generally defined as carbon catabolite repression. The current lack of simultaneous mixed-sugar utilization limits achievable titers, yields, and productivities. Therefore, the development of microbial platforms capable of fermenting mixed sugars simultaneously from biomass hydrolysates is essential for economical industry-scale production, particularly for ...


Multi-Responsive Macromolecular Coacervate Complexes For Efficient Biomolecular Encapsulation And Controlled Release, Adrian Lorenzana 2019 Wayne State University

Multi-Responsive Macromolecular Coacervate Complexes For Efficient Biomolecular Encapsulation And Controlled Release, Adrian Lorenzana

ROEU 2018-19

The project investigates the mechanistic (entropic) formation of organic-inorganic coacervates with improved mechanical strength. We seek to control and modify material properties and stimuli-responsive characteristics of hybrid coacervates, and to explore new practical applications of organic-inorganic coacervate complexes, i.e. (bio)separation, wastewater treatment, drug delivery, battery electrolyte and electrodes.


Synthesis Of Drug Encapsulated Chitosan-Heparin Based Nanoparticles And Release Study, Trent Richwine 2019 The University of Akron

Synthesis Of Drug Encapsulated Chitosan-Heparin Based Nanoparticles And Release Study, Trent Richwine

Williams Honors College, Honors Research Projects

The main objective of this honor’s project is to encapsulate drugs with different molecular weights inside of chitosan-heparin based nanoparticles (Cs-Hep NPs). Three different drugs with low (NNC), medium (IFN-γ), and large (BSA) molecular weights were chosen in order to compare all types of drugs and how they react with the nanoparticles. Analysis of particle size, zeta potential, and a release study was performed. The release took place over a 168-hour time period and liquid samples were taken throughout. The hypothesis was that the molecular weight of the drug will correlate to the physical properties of the nanoparticles. The ...


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