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Biochemical and Biomolecular Engineering Commons

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Influence Of Flow Rate, Nozzle Speed, Pitch And The Number Of Passes On The Thickness Of S1805 Photoresist In Suss Microtec As8 Spray Coater, Rohan Sanghvi, Gyuseok Kim 2019 Singh Center for Nanotechnology

Influence Of Flow Rate, Nozzle Speed, Pitch And The Number Of Passes On The Thickness Of S1805 Photoresist In Suss Microtec As8 Spray Coater, Rohan Sanghvi, Gyuseok Kim

Tool Data

S1805 positive photoresist has been deposited on single crystalline Si wafers using a Suss MicroTec Alta Spray. The influence of flow rate, nozzle speed, pitch and number of passes on the thickness of the photoresist was studied. Results show that the thickness of S1805 is linearly proportional to the flow rate and number of passes, and inversely proportional to the nozzle speed and pitch.


Immobilization Of The Influenza A M2 Transmembrane Peptide In Virus Envelope−Mimetic Lipid Membranes: A Solid-State Nmr Investigation, Wenbin Luo, Sarah D. Cady, Mei Hong 2019 Iowa State University

Immobilization Of The Influenza A M2 Transmembrane Peptide In Virus Envelope−Mimetic Lipid Membranes: A Solid-State Nmr Investigation, Wenbin Luo, Sarah D. Cady, Mei Hong

Sarah Cady

The dynamic and structural properties of membrane proteins are intimately affected by the lipid bilayer. One property of membrane proteins is uniaxial rotational diffusion, which depends on the membrane viscosity and thickness. This rotational diffusion is readily manifested in solid-state NMR spectra as characteristic line shapes and temperature-dependent line narrowing or broadening. We show here that this whole-body uniaxial diffusion is suppressed in lipid bilayers mimicking the composition of eukaryotic cell membranes, which are rich in cholesterol and sphingomyelin. We demonstrate this membrane-induced immobilization on the transmembrane peptide of the influenza A M2 (AM2-TM) proton channel protein. At physiological temperature ...


Influence Of Naoh Concentration On Transfer Process Of Graphene, Francisco Saldana, Chengyu Wen, George Patrick Watson 2019 Singh Center for Nanotechnology

Influence Of Naoh Concentration On Transfer Process Of Graphene, Francisco Saldana, Chengyu Wen, George Patrick Watson

Protocols and Reports

The process of transferring a monolayer of graphene using two different concentrations of sodium hydroxide (NaOH) solution unto a silicon dioxide (SiO2) coated Si chip using electrochemistry was performed. The transfer process is crucial for the delamination of a continuous graphene monolayer film from copper foil. After examining and inspecting the integrity of the graphene monolayer, it was observed that the lower concentration to NaOH led to slower rate of hydrogen bubble generation; this condition was found to be less destructive and yielded a graphene film with fewer visible tears.


Evaluation Of Biohydrogen Production From Co-Fermentation Of Carbohydrates And Proteins, Emmanuel Andrew Tepari Jr, 2019 The University of Western Ontario

Evaluation Of Biohydrogen Production From Co-Fermentation Of Carbohydrates And Proteins, Emmanuel Andrew Tepari Jr,

Electronic Thesis and Dissertation Repository

The aim of this study was to examine volatile fatty acid (VFA) production from a proteinaceous substrate, bovine serum albumin (BSA) for a pH range of 5 – 9, and to further assess its impact on hydrogen production in a co-fermentation process using starch and BSA at different ratios. The established optimum conditions for VFA production from BSA were an initial pH of 8, incubation time of 3 days and an operating temperature of 37 ℃. Using these fermentation conditions, the stoichiometric reactions that describe the anaerobic degradation of BSA were investigated. A methodology that describes organic acid production from BSA by ...


Renal Specific Rnai Delivery By Fibrillar Nanoparticle Excipients, Sam Wong 2019 The Graduate Center, City University of New York

Renal Specific Rnai Delivery By Fibrillar Nanoparticle Excipients, Sam Wong

All Dissertations, Theses, and Capstone Projects

RNA interference (RNAi) is a powerful tool to manipulate the phenotype of an organism by silencing the expression of specific genes and is viewed as a highly promising platform for treating undruggable targets and disorders where small molecule drugs and antibodies would fail. However, development of RNAi based therapies has faced major barriers including cellular and tissue-specific uptake of the Small Interfering RNA (siRNA). Utilizing different nanoparticles as RNAi excipients, cellular uptake and gene silencing potency can be greatly improved. The research in Dr. McDevitt groups has fibrillar carbon nanotubes (CNT) as carriers for siRNA for gene silencing in vitro ...


Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts 2019 University of Arkansas, Fayetteville

Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts

Theses and Dissertations

Volumetric muscle loss overwhelms skeletal muscle’s ordinarily capable regenerative machinery, resulting in fibrosis and severe functional deficits which have defied clinical repair strategies. My work spans the design and preclinical evaluation of implants intended to drive the cell community of injured muscle toward a regenerative state, as well as the development of an understanding of the molecular responses of this cell community to biomaterial interventions. I demonstrate a new class of biomaterial by leveraging the productive capacity of sacrificial hollow fiber membrane cell culture; I show specifically that unique threads of whole extracellular matrix can be isolated by solvent ...


Characterization Of Tetrazolium Salts And Formazans Using Computational Chemistry For Radiochromic Dosimetry, Teshome G. Bedada 2019 The University of Western Ontario

Characterization Of Tetrazolium Salts And Formazans Using Computational Chemistry For Radiochromic Dosimetry, Teshome G. Bedada

Electronic Thesis and Dissertation Repository

Computational chemistry is a powerful tool to model different materials and predict their physical and chemical properties. The goal of this thesis was to apply different computational chemistry tools to model the properties of two classes of closely related organic compounds: formazans and tetrazolium salts for ionizing radiation dosimetry in water-based gels, whereby the absorbed dose is registered by optical detection of formazan formation from tetrazolium salts. The structural parameters of phenyl and benzothiazolyl-substituted tetrazolium salts and formazans were computed using the APFD density functional theory (DFT) model and 6-311+G(2d,p) basis set. Conformational searching of stereoisomers (TSCA ...


K-Fit: Parameterizing Kinetic Models Of Metabolism Using Multiple Fluxomic Datasets, Costa Maranas 2019 Penn State University, USA

K-Fit: Parameterizing Kinetic Models Of Metabolism Using Multiple Fluxomic Datasets, Costa Maranas

Biochemical and Molecular Engineering XXI

Kinetic models predict the metabolic flows by directly linking metabolite concentrations and enzyme levels to reaction fluxes. Robust parameterization of organism-level kinetic models that faithfully reproduce the effect of different genetic or environmental perturbations remains an open challenge due to the intractability of existing algorithms. This paper introduces K-FIT, an accelerated kinetic parameterization workflow that leverages a novel decomposition approach to identify steady-state fluxes in response to genetic perturbations followed by a gradient-based update of kinetic parameters until predictions simultaneously agree with the fluxomic data in all perturbed metabolic networks. The applicability of K-FIT to large-scale models is demonstrated by ...


The Statistics Of Directed Evolution: From Library Generation To High Throughput Screens, Keith Tyo 2019 Northwestern University, USA

The Statistics Of Directed Evolution: From Library Generation To High Throughput Screens, Keith Tyo

Biochemical and Molecular Engineering XXI

Directed evolution has been recognized as a powerful approach to creating enzymes and cells with desirable properties, such as growth on a new substrate, thermotolerance, or even novel reaction mechanisms. The directed evolution approach is to generate diversity through genetic mutations and screen the diversity for mutants that have improved properties compared to the parent. This diversification and screening can be repeated to generate mutants with superior properties.

While the concept is straightforward, the devil is in the details. How should I create diversity (error prone PCR, mutagenic primers, transposons…)? How much diversity do I need (103, 106 ...


Advanced Technologies And Computational Modeling In Continuous Bioprocessing Heather Brooke, Pall Corporation, Usa, Heather Brooke, Edita Botonjic-Sehic 2019 Pall Corporation, USA

Advanced Technologies And Computational Modeling In Continuous Bioprocessing Heather Brooke, Pall Corporation, Usa, Heather Brooke, Edita Botonjic-Sehic

Biochemical and Molecular Engineering XXI

Biotech companies have been emphasizing the implementation and utilization of process analytical technology (PAT) and chemometrics in biomanufacturing. Advanced technologies and computation modeling have contributed significantly in making this capability come to life. Although there are still gaps in the area of advanced analytics for the process, computation modeling has started to contribute towards process characterization, which will lead to real-time process control. PAT and modeling infrastructure can be utilized in integrated continuous bioprocessing to gain better process understating, reduce production cycle time, improve yield, advance efficiency by converting from a batch process into a continuous process, and reduce cost ...


Biologicalisation: A Nature-Based Digital Manufacturing Revolution, William Whitford 2019 GE Healthcare, USA

Biologicalisation: A Nature-Based Digital Manufacturing Revolution, William Whitford

Biochemical and Molecular Engineering XXI

Industry 4.0 is changing our manufacturing concepts [1]. Biologicalisation is the use of 4.0 principals in concert with biological and bio-inspired materials, chemistries and functions to support an efficient and sustainable manufacturing schema. From product design, to development and manufacturing, biomimetic product designs and bio-integrated manufacturing systems describe this biological transformation of manufacturing [2].

Please click Additional Files below to see the full abstract.


Conference Program, Christina Chan, Mattheos Koffas, Steffen Schaffer, Rashmi Kshirsagar 2019 Michigan State University

Conference Program, Christina Chan, Mattheos Koffas, Steffen Schaffer, Rashmi Kshirsagar

Biochemical and Molecular Engineering XXI

No abstract provided.


Beneficial Reuse Of Industrial Co2 Emissions Using A Microalgae Photobioreactor: Waste Heat Utilization Assessment, Daniel T. Mohler, Michael H. Wilson, Zhen Fan, John G. Groppo, Mark Crocker 2019 University of Kentucky

Beneficial Reuse Of Industrial Co2 Emissions Using A Microalgae Photobioreactor: Waste Heat Utilization Assessment, Daniel T. Mohler, Michael H. Wilson, Zhen Fan, John G. Groppo, Mark Crocker

Center for Applied Energy Research Faculty Publications

Microalgae are a potential means of recycling CO2 from industrial point sources. With this in mind, a novel photobioreactor (PBR) was designed and deployed at a coal-fired power plant. To ascertain the feasibility of using waste heat from the power plant to heat algae cultures during cold periods, two heat transfer models were constructed to quantify PBR cooling times. The first, which was based on tabulated data, material properties and the physical orientation of the PBR tubes, yielded a range of heat transfer coefficients of 19–64 W m−2 K−1 for the PBR at wind speeds of ...


Promoting Microbial Utilization Of Phenolic Substrates From Bio-Oil, Kirsten Davis, Marjorie R. Rover, Davinia Salvachúa, Ryan G. Smith, Gregg T. Beckham, Zhiyou Wen, Robert C. Brown, Laura R. Jarboe 2019 Iowa State University

Promoting Microbial Utilization Of Phenolic Substrates From Bio-Oil, Kirsten Davis, Marjorie R. Rover, Davinia Salvachúa, Ryan G. Smith, Gregg T. Beckham, Zhiyou Wen, Robert C. Brown, Laura R. Jarboe

Mechanical Engineering Publications

The economic viability of the biorefinery concept is limited by the valorization of lignin. One possible method of lignin valorization is biological upgrading with aromatic-catabolic microbes. In conjunction, lignin monomers can be produced by fast pyrolysis and fractionation. However, biological upgrading of these lignin monomers is limited by low water solubility. Here, we address the problem of low water solubility with an emulsifier blend containing approximately 70 wt% Tween® 20 and 30 wt% Span® 80. Pseudomonas putida KT2440 grew to an optical density (OD600) of 1.0 ± 0.2 when supplied with 1.6 wt% emulsified phenolic monomer-rich product produced ...


Modeling The Interplay Between Photosynthesis, Co2 Fixation, And The Quinone Pool In A Purple Non-Sulfur Bacterium, Adil Alsiyabi 2019 University of Nebraska - Lincoln

Modeling The Interplay Between Photosynthesis, Co2 Fixation, And The Quinone Pool In A Purple Non-Sulfur Bacterium, Adil Alsiyabi

Chemical & Biomolecular Engineering Theses, Dissertations, & Student Research

Rhodopseudomonas palustris CGA009 is a purple non-sulfur bacterium (PNSB) that can fix CO2 and nitrogen or break down organic compounds for its carbon and nitrogen requirements. Light, inorganic, and organic compounds can all be used for its source of energy. Excess electrons produced during its metabolic processes can be exploited to produce hydrogen gas or biodegradable polyesters (polyhydroxybutyrate). A genome-scale metabolic model of the bacterium was reconstructed to study the interactions between photosynthesis, carbon dioxide fixation, and the redox state of the quinone pool. A comparison of model-predicted flux values with published in vivo MFA fluxes resulted in predicted ...


Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian 2019 University of Nebraska - Lincoln

Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian

Chemical & Biomolecular Engineering Theses, Dissertations, & Student Research

The development of recombinant-based liquid fibrin tissue sealants having enhanced hemostatic and wound healing properties will involve understanding as yet not well characterized interactions between fibrinogen, fibrin (Fbn) factor XIII, thrombin and fibronectin. We study these phenomena in the context of comparing plasma derived fibrinogen to recombinant fibrinogen (rFI) produced in the milk of transgenic cows. An abundance of purified γγ and γγ’ FI subspecies enables detailed study of γγ or γγ’ biomonomer and their respective Fbn biopolymer formation as having different substrate behaviors of activated plasma derived factor XIII (pFXIIIa2b2). High pressure size exclusion (HPSEC) and ...


Stress Response To Co2 Deprivation By Arabidopsis Thaliana In Plant Cultures, Souvik Banerjee, Oskar Siemianowski, Meiling Liu, Kara R. Lind, Xinchun Tian, Dan Nettleton, Ludovico Cademartiri 2019 Iowa State University

Stress Response To Co2 Deprivation By Arabidopsis Thaliana In Plant Cultures, Souvik Banerjee, Oskar Siemianowski, Meiling Liu, Kara R. Lind, Xinchun Tian, Dan Nettleton, Ludovico Cademartiri

Dan Nettleton

After being the standard plant propagation protocol for decades, cultures of Arabidopsis thaliana sealed with Parafilm remain common today out of practicality, habit, or necessity (as in co-cultures with microorganisms). Regardless of concerns over the aeration of these cultures, no investigation has explored the CO2 transport inside these cultures and its effect on the plants. Thereby, it was impossible to assess whether Parafilm-seals used today or in thousands of older papers in the literature constitute a treatment, and whether this treatment could potentially affect the study of other treatments.For the first time we report the CO2concentrations in Parafilm-sealed cultures ...


Biomethane Production From Distillery Wastewater, Zachary Christman 2019 University of Nebraska - Lincoln

Biomethane Production From Distillery Wastewater, Zachary Christman

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Distillery wastewater treatment is a great ecological problem, for example, India produces 2.7 billion liters of alcohol that results in 40 billion liters of wastewater. However, this material can be seen as a resource since 11 million cubic meters of biogas at 60% methane could be produced in addition to cleaning the water. The distillery has two options of what to do with the biogas. The first is to use the biogas to fuel the distillery making the production plant more energy efficient and removing some of the need to buy natural gas. The other is to upgrade the ...


Conversion Of Omega-3 Fatty Acids From Algae Biomass Produced Biodiesel, Matthew Peters, Jacquelyn Stokes, Ryan Tu 2019 University of Pennsylvania

Conversion Of Omega-3 Fatty Acids From Algae Biomass Produced Biodiesel, Matthew Peters, Jacquelyn Stokes, Ryan Tu

Senior Design Reports (CBE)

As the effects of global warming continue to escalate, carbon-neutral fuels are becoming sought after alternatives to traditional fossil fuels. Biodiesel is a promising source of carbon-neutral fuel that can be produced from organisms such as algae. A new process has been proposed by Yadav for algal oil extraction using sonic waves and carbon dioxide microbubbles (Yadav et al., 2019). Although a novel idea, it currently struggles to compete economically with other types of biodiesel production as well as traditional food sources. However, a small subsection of the FAMEs is able to be further processed into Omega-3 fatty acids. These ...


Chemical Recycling Of Plastics By Dissolution, Pensiri Naviroj, James Treacy, Conrad Urffer 2019 University of Pennsylvania

Chemical Recycling Of Plastics By Dissolution, Pensiri Naviroj, James Treacy, Conrad Urffer

Senior Design Reports (CBE)

There is significant upside potential for the recycling of plastic wastes, especially for waste polyethylene plastic bags. Plastic containers and packaging have the most tonnage of waste plastics sent to landfills, and there are limited number of communities collecting single-use polyethylene plastics bags for recycling. This project explores the dissolution-precipitation technique as a viable plastics recycling method. Beginning with a feed assumed to be primarily low density polyethylene (LDPE), plastic bag waste is cleaned and 50 tons per day of this cleaned LDPE waste enters the main process. The proposed design includes a dissolution and precipitation vessel. The design is ...


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