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Biochemical and Biomolecular Engineering Commons

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Physical And Chemical Characteristics Of Supramolecular Complexes Of Glycirrizinic Acid With Phytohormones, DJURAEV Tulqin, ESANOV Rakhmatillo, GAFUROV Makhmudjon, KUSHIEV Khabibjon 2020 Gulistan State University

Physical And Chemical Characteristics Of Supramolecular Complexes Of Glycirrizinic Acid With Phytohormones, Djuraev Tulqin, Esanov Rakhmatillo, Gafurov Makhmudjon, Kushiev Khabibjon

CHEMISTRY AND CHEMICAL ENGINEERING

The process of formation of supramolecular complexes of glycyrrhizinic acid with some phytohormones (indolylacetic acid, indolylbutyric acid, α-naphthyl acetic acid and kinetin) were studied with the aim of creation the plant growth stimulator. The complexes structure was studied using IR and UV spectroscopy methods. The prepared compounds described by some physicochemical parameters. The stability constants and stoichiometric composition of complexes were determined in an aqueous solution at pH of 7,2 using UV spectrometry method. In result, all complexes have composition as 1:1 ratio, except the kinetin complex which has 2:1 ratio. The stability constants were equal: KGA ...


Study Of The Process For The Removal Of Dyeing Matters Of The Plant Occimum Basilicum L. Lamiaceae, USMONJONOVA Khulkar, ATKHAMOVA Saida, DODAEV Kuchkor 2020 Tashkent Chemical-Technological Institute

Study Of The Process For The Removal Of Dyeing Matters Of The Plant Occimum Basilicum L. Lamiaceae, Usmonjonova Khulkar, Atkhamova Saida, Dodaev Kuchkor

CHEMISTRY AND CHEMICAL ENGINEERING

The aim of the work is dedicated isolation of anthocyanins by ethanol from herbal plant of Occimum basilicum L. Lamiaceae basis. The were studied influencing of temperature, concentration of ethanol to isolation of anthocyanins. There were passed investigations on studying influence level of heating and hydromodule system of rav material-solvent to maximal isolating of anthocyanins. The colors define by fotokolorometrical method at wave length 400-600 nm. There are shown at 540 nm maximal peak of anthocyanins and it is geven as a grapfical diagram. From the obtained depends on content of alcohol in aquatic solution from outlet of colors is ...


Study Of Recombinant Protein Fractions From The Bombyx Mori And Pichia Pastoris Using "Native Page" Method, Abdurhakhmanov aloliddin, Sasmakov Sobirdjan, Khasanov Shuhrat, Ashirov Оybek 2020 Institute of the Chemistry of Plant Substances

Study Of Recombinant Protein Fractions From The Bombyx Mori And Pichia Pastoris Using "Native Page" Method, Abdurhakhmanov Aloliddin, Sasmakov Sobirdjan, Khasanov Shuhrat, Ashirov Оybek

CHEMISTRY AND CHEMICAL ENGINEERING

We have studied the recombinant protein fractions of ̴ 24-34 kDa obtained from Bombyx mori - silkworm larvae and yeast Pichia pastoris. Fractions, containing the recombinant proteins by gel-electrophoresis using the “Native PAGE” method, which allows for differentiation of proteins according to their natural (surface) charge and conformational properties were studied. The results showed that the recombinant protein fraction from Bombyx mori (̴ 34 kDa) consisted of four components and fraction from Pichia pastoris (̴ 24 kDa) consisted one component. The most optimal conditions for the “Native PAGE”- the main gel: Tris HCl (pH-8.8) 244 mM, Bis-Acrylamide 10%, TEMED 0.08%, APS 0 ...


Metabolic Engineering Of An Acid-Tolerant Yeast Strain Pichia Kudriavzevii For Itaconic Acid Production, Wan Sun, Ana Vila-Santa, Na Liu, Tanya Prozorov, Dongming Xie, Nuno Torres Faria, Frederico Castelo Ferreira, Nuno Pereira Mira, Zengyi Shao 2020 Iowa State University and Ames Laboratory

Metabolic Engineering Of An Acid-Tolerant Yeast Strain Pichia Kudriavzevii For Itaconic Acid Production, Wan Sun, Ana Vila-Santa, Na Liu, Tanya Prozorov, Dongming Xie, Nuno Torres Faria, Frederico Castelo Ferreira, Nuno Pereira Mira, Zengyi Shao

Chemical and Biological Engineering Publications

Itaconic acid (IA), or 2-methylenesuccinic acid, has a broad spectrum of applications in the biopolymer industry owing to the presence of one vinyl bond and two acid groups in the structure. Its polymerization can follow a similar mechanism as acrylic acid but additional functionality can be incorporated into the extra beta acid group. Currently, the bio-based production of IA in industry relies on the fermentation of the filamentous fungus Aspergillus terreus. However, the difficulties associated with the fermentation undertaken by filamentous fungi together with the pathogenic potential of A. terreus pose a serious challenge for industrial-scale production. In recent years ...


Prada: Portable Reusable Accurate Diagnostics With Nanostar Antennas For Multiplexed Biomarker Screening, Xiaona Wen, Yu-Chuan Ou, Holly F. Zarick, Xin Zhang, Anthony B. Hmelo, Quinton J. Victor, Eden P. Paul, Joseph M. Slocik, Rajesh R. Naik, Leon M. Bellan, Eugene C. Lin, Rizia Bardhan 2020 Vanderbilt University

Prada: Portable Reusable Accurate Diagnostics With Nanostar Antennas For Multiplexed Biomarker Screening, Xiaona Wen, Yu-Chuan Ou, Holly F. Zarick, Xin Zhang, Anthony B. Hmelo, Quinton J. Victor, Eden P. Paul, Joseph M. Slocik, Rajesh R. Naik, Leon M. Bellan, Eugene C. Lin, Rizia Bardhan

Chemical and Biological Engineering Publications

Precise monitoring of specific biomarkers in biological fluids with accurate biodiagnostic sensors is critical for early diagnosis of diseases and subsequent treatment planning. In this work, we demonstrated an innovative biodiagnostic sensor, portable reusable accurate diagnostics with nanostar antennas (PRADA), for multiplexed biomarker detection in small volumes (~50 μl) enabled in a microfluidic platform. Here, PRADA simultaneously detected two biomarkers of myocardial infarction, cardiac troponin I (cTnI), which is well accepted for cardiac disorders, and neuropeptide Y (NPY), which controls cardiac sympathetic drive. In PRADA immunoassay, magnetic beads captured the biomarkers in human serum samples, and gold nanostars (GNSs) “antennas ...


Lithium Recovery Process From Spent Batteries, Nicholas A. Ross, Lacey Roberts, Jordan Leith, Woodi Woodland 2020 University of Tennessee, Knoxville

Lithium Recovery Process From Spent Batteries, Nicholas A. Ross, Lacey Roberts, Jordan Leith, Woodi Woodland

Chancellor’s Honors Program Projects

No abstract provided.


Production Of Dimethyl Ether (Dme) For Transportation Fuel, Anita S. Yang, Carl V. Antrassian, Julian D. Kurtzman 2020 University of Pennsylvania

Production Of Dimethyl Ether (Dme) For Transportation Fuel, Anita S. Yang, Carl V. Antrassian, Julian D. Kurtzman

Senior Design Reports (CBE)

Dimethyl Ether (DME) is a proposed alternative to diesel fuel that is being looked into by car and truck manufacturers worldwide. The current market, based almost completely in China, is primed for growth and a U.S. based DME total plant that is economical and environmentally feasible stands to pave the way for America’s DME market, especially since states such as California have approved DME for use as vehicle fuel (Fuel Smarts). Conventionally, the DME is produced by feeding Methanol into a xed-bed gas-phase reactor over a ɣ-alumina catalyst (Dimian et al). Using this process and normal operating conditions ...


Rare Earth Element Recovery From Phosphogypsum Using A Biolixiviant, Angelica M. Padilla, Andreea-Maria Pana, Roshan A. Patel 2020 University of Pennsylvania

Rare Earth Element Recovery From Phosphogypsum Using A Biolixiviant, Angelica M. Padilla, Andreea-Maria Pana, Roshan A. Patel

Senior Design Reports (CBE)

This project describes the process of recovering of rare earth elements (REE) from phosphogypsum (PG) waste using a novel biolixviant produced by a bacteria strain known as Glucanobacter oxydans and is inspired by novel research occurring at Idaho National Laboratories. This report details design of a plant which has the capacity to produce the biolixiviant through fermentation, use this biolixiviant to leach REE from a solid waste such as PG, and recover the REE in oxide form through crystallization, filtration, and subsequent high temperature decomposition. The plant has the capability of processing nearly 1 million MT of PG a year ...


Production Of Astaxanthin Using Modified E. Coli Cells, Christina M. Eng, Connor S. Gallagher, Maura K. Kimmel 2020 University of Pennsylvania

Production Of Astaxanthin Using Modified E. Coli Cells, Christina M. Eng, Connor S. Gallagher, Maura K. Kimmel

Senior Design Reports (CBE)

There are several promising markets for naturally synthesized Astaxanthin, a carotenoid found in krill, shrimp, salmon, and other marine life that imparts to these creatures a pink coloring of the flesh and has demonstrated human health-promoting anti-inflammatory and antioxidant activity. Compared to Astaxanthin produced through chemical synthesis, Astaxanthin synthesized through natural fermentative processes (in yeast and algae) is esterified, imparting greater antioxidant activity as well as bioavailability and making it the preferred ingredient for nutraceutical formulations. Additionally, as consumer preferences trend toward natural production processes free from the use of petrochemical solvents which may pose hazards to health hazards, fermentative ...


Waste Co₂ To Co For Energy Storage, Vignesh C. Bhethanabotla, Joseph C. Dennis, Mavis Athene U Chen 2020 University of Pennsylvania

Waste Co₂ To Co For Energy Storage, Vignesh C. Bhethanabotla, Joseph C. Dennis, Mavis Athene U Chen

Senior Design Reports (CBE)

In this report, we present a process design for the storage of electricity using solid oxide fuel cell (SOFC) technology to convert electrical energy to chemical fuel via the reduction of carbon dioxide. The goal of this project was to design a system that could alleviate the issues around the intermittent nature of renewable energy production, which must phase out the use of fossil fuels in the future. The overall process was developed for two storage strategies, and the economics feasibility of the design is considered and reported. The key aspect of this process is its reversible nature. Both the ...


Enzyme Production Using An Extremophilic Biocatalyst, Adithya Sriram, Sean M. Begane, Jazriel A. Ortega 2020 University of Pennsylvania

Enzyme Production Using An Extremophilic Biocatalyst, Adithya Sriram, Sean M. Begane, Jazriel A. Ortega

Senior Design Reports (CBE)

Bio-detergents are useful in many markets because they contain enzymes that can break down proteins and in turn enhance stain removal. However, many of these enzymes deactivate at high temperatures so sterilization and stain removal must be done in two separate processes. This process produces extremozymes, enzymes derived from extremophilic microorganisms that have optimal activity and stability at harsh conditions, to be used in bio-detergents. The thermostable characteristics of this enzyme allow it to accomplish high levels of protein breakdown at temperatures conducive to sterilization. It is expected that this characteristic provides a 15% price premium over the leading incumbant ...


Batch Production Of A Potent Small Molecule Active Pharmaceutical Ingredient, Isuru N. Amarasekera, Jinwan Cho, Jason Y. Li 2020 University of Pennsylvania

Batch Production Of A Potent Small Molecule Active Pharmaceutical Ingredient, Isuru N. Amarasekera, Jinwan Cho, Jason Y. Li

Senior Design Reports (CBE)

Small molecule Active Pharmaceutical Ingredients (APIs) have become increasingly relevant in cancer treatment due to their efficacy, targeted treatment, and clinical value. Commonly manufactured in a batch pharmaceutical process, APIs must satisfy Critical Quality Attributes (CQAs) including chemical purity and physical properties. This process involves a multitude of steps, components, and equipment that are optimized to produce an API in a timely and cost-efficient manner. Here, we consider the process and facility design of a batch production of Halfaxia, a new potent anti-cancer drug from Johnson & Johnson. The process begins with a reaction of a starting material and a second ...


Renewable Ammonia, Kolbein A. Finsnes, James P. Kwon, Dakota M. Wallach 2020 University of Pennsylvania

Renewable Ammonia, Kolbein A. Finsnes, James P. Kwon, Dakota M. Wallach

Senior Design Reports (CBE)

Ammonia is one of the most widely used chemicals that is commercially produced today given the wide need for fertilizer to sustain the world’s ever-growing populations. Given the high world demand for ammonia, which increases every day, one can see how beneficial to the environment that a zero emission large-scale ammonia plant would be. Through the use of energy from Norwegian wind farms, which produce an excess of energy during off-peak hours, our plant design seeks to turn this wasted energy into useful ammonia products at a production rate of 67.2 kmol/hr.

The design of this ammonia ...


Mixed Plastics Waste To Ethylene And Propylene Feedstocks, Promise O. Adebayo-Ige, Sarah M. Engelhardt, Matthew C. Larson 2020 University of Pennsylvania

Mixed Plastics Waste To Ethylene And Propylene Feedstocks, Promise O. Adebayo-Ige, Sarah M. Engelhardt, Matthew C. Larson

Senior Design Reports (CBE)

Circular recycle of waste plastic holds significant environmental benefit in reducing the need for crude oil feed to produce plastic monomers and in addressing massive global accumulation of plastic waste. A two-stage cracking process is here explored for the reduction of long-chain polyethylene (PE), polypropylene (PP), and polystyrene (PS) to ethylene and propylene. The reaction yields useful byproducts, such as liquid fuel used to sustain the high energy demands of the process, and pressurized steam. A feed of 70 MT/day of PE, PP, and PS is assumed to be treated first in a rotary kiln pyrolysis reactor and secondly ...


Commercial Process For The Conversion Of Graphite To Graphite Oxide, Alexander Goodisman, Yash Jain, Dong Bien Kwon 2020 University of Pennsylvania

Commercial Process For The Conversion Of Graphite To Graphite Oxide, Alexander Goodisman, Yash Jain, Dong Bien Kwon

Senior Design Reports (CBE)

With steel and other everyday materials reaching their limits in terms of structural integrity and chemical properties, new materials are needed to provide the next breakthroughs in construction and R&D. Graphene provides incredible strengths and elasticities along with amazing electrical and thermal conductivity. Graphene based materials, the so-called supermaterials, are often limited by the lack of availability due to cost and volume. This report discusses the first large scale production of an important precursor to graphene, graphite oxide (GrO). Based on improvements of Hummer’s method, this project analyses the potential challenges associated with dealing with large quantities of ...


Production Of Dl-Methionine From Corn Syrup Via Biosynthesis With Corynebacterium Glutamicum, Dayoung Shin, Yvonne N. Szustakiewicz, Allison P. Walter 2020 University of Pennsylvania

Production Of Dl-Methionine From Corn Syrup Via Biosynthesis With Corynebacterium Glutamicum, Dayoung Shin, Yvonne N. Szustakiewicz, Allison P. Walter

Senior Design Reports (CBE)

DL-methionine is an important feed additive for poultry and swine diets. The amino acid is not produced by animals so it can only be obtained through diet. Methionine is important for protein growth and helps the health of livestock. Recent increase in meat consumption in Latin America and Asia, along with a growing consumer concern of animal welfare, has driven a growth in the methionine market. The methionine market is predicted to continue to grow for at least another five years (Methionine Market 2019). The proposed design is for a process that produces DL-methionine by fermentation with the bacteria, Corynebacterium ...


Design Of Car-T Cell Manufacturing Process, Liam J. Bartie, Lauren R. Duhamel, Ruby C. Pan 2020 University of Pennsylvania

Design Of Car-T Cell Manufacturing Process, Liam J. Bartie, Lauren R. Duhamel, Ruby C. Pan

Senior Design Reports (CBE)

CAR T-cell therapy is at the frontier of personalized immunotherapy. It is a therapy that essentially reprograms a patient’s own T-cells to attack certain blood cancers. A sample of a patient's T cells are collected from the blood, then modified to produce chimeric antigen receptors (CARs) on their surface. When these CAR T cells are reinfused into the patient, the new receptors enable them to latch onto a specific antigen on the patient's tumor cells and kill them, ideally sending the patient into remission and essentially curing their cancer. Currently, CAR T-cell therapy is FDA approved as ...


Process Water Recycling, Adrian Barron, Josiah Snider, Kyle Serpe 2020 Olivet Nazarene University

Process Water Recycling, Adrian Barron, Josiah Snider, Kyle Serpe

Scholar Week 2016 - present

The Millipore Sigma-Kankakee Site uses high volumes of water for many operations. One operation utilizes approximately 3600 L of water per batch every couple days and more than 3.8MM liters of water per year. The water is utilized as a component of the buffer washes used in the extraction process for cholesterol that involves various other components such as silica and ions such as sodium, sulfur, and phosphate. The washes that go through the process involve these components and large amounts of water that are then drained out of the facility. This volume is roughly 7% of the site ...


Effective Dispersion Of Mgo Nanostructure On Biochar Support As A Basic Catalyst For Glucose Isomerization, Season S. Chen, Yang Cao, Daniel C.W. Tsang, Jean-Philippe Tessonnier, Jin Shang, Deyi Hou, Zhengtao Shen, Shicheng Zhang, Yong Sik Ok, Kevin C.-W. Wu 2020 The Hong Kong Polytechnic University and National Taiwan University

Effective Dispersion Of Mgo Nanostructure On Biochar Support As A Basic Catalyst For Glucose Isomerization, Season S. Chen, Yang Cao, Daniel C.W. Tsang, Jean-Philippe Tessonnier, Jin Shang, Deyi Hou, Zhengtao Shen, Shicheng Zhang, Yong Sik Ok, Kevin C.-W. Wu

Chemical and Biological Engineering Publications

Glucose isomerization to fructose is one of the most important reactions in the field of biomass valorization. We demonstrate wood waste valorization with MgCl2 salt to synthesize an environment-friendly catalyst (i.e., MgO-biochar), which exhibits effective glucose-to-fructose isomerization with over 30% fructose yield and 80% selectivity at only 100 °C for 30 min in water as a green medium. This study highlights that one-step synthesis can effectively disperse and tether MgO nanostructures to the biochar matrix, which displays a significant reduction of Mg leaching compared to MgO-biochars produced by two-step synthesis and pure MgO. The MgCl2 acts as a porogen ...


High-Throughput Synthesis And Screening Of Rapidly-Degrading Polyanhydride Nanoparticles, Adam S. Mullis, Sarah J. Jacobson, Balaji Narasimhan 2020 Iowa State University

High-Throughput Synthesis And Screening Of Rapidly-Degrading Polyanhydride Nanoparticles, Adam S. Mullis, Sarah J. Jacobson, Balaji Narasimhan

Chemical and Biological Engineering Publications

Combinatorial techniques can accelerate the discovery and development of polymeric nanodelivery devices by pairing high-throughput synthesis with rapid materials characterization. Biodegradable polyanhydrides demonstrate tunable release, high cellular internalization, and dose sparing properties when used as nanodelivery devices. This nanoparticle platform shows promising potential for small molecule drug delivery, but the pace of understanding and rational design of these nanomedicines is limited by the low throughput of conventional characterization. This study reports the use of a high-throughput method to synthesize libraries of a newly synthesized, rapidly-eroding polyanhydride copolymer based on 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) and sebacic acid (SA) monomers ...


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