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A Single Dose Polyanhydride-Based Nanovaccine Against Paratuberculosis Infection, Akanksha Thukral, Kathleen Ross, Chungyi Hansen, Yashdeep Phanse, Balaji Narasimhan, Howard Steinberg, Adel M. Talaat 2020 University of Wisconsin, Madison

A Single Dose Polyanhydride-Based Nanovaccine Against Paratuberculosis Infection, Akanksha Thukral, Kathleen Ross, Chungyi Hansen, Yashdeep Phanse, Balaji Narasimhan, Howard Steinberg, Adel M. Talaat

Chemical and Biological Engineering Publications

Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) causes Johne’s disease in ruminants and is characterized by chronic gastroenteritis leading to heavy economic losses to the dairy industry worldwide. The currently available vaccine (inactivated bacterin in oil base) is not effective in preventing pathogen shedding and is rarely used to control Johne’s disease in dairy herds. To develop a better vaccine that can prevent the spread of Johne’s disease, we utilized polyanhydride nanoparticles (PAN) to encapsulate mycobacterial antigens composed of whole cell lysate (PAN-Lysate) and culture filtrate (PAN-Cf) of M. paratuberculosis. These nanoparticle-based vaccines (i.e., nanovaccines) were well ...


Multimodal Multiplexed Immunoimaging With Nanostars To Detect Multiple Immunomarkers And Monitor Response To Immunotherapies, Yu-Chuan Ou, Xiaona Wen, Christopher A. Johnson, Daniel Shae, Oscar D. Ayala, Joseph A. Webb, Eugene C. Lin, Rossane C. DeLapp, Kelli L. Boyd, Ann Richmond, Anita Mahadevan-Jansen, Marjan Rafat, John T. Wilson, Justin M. Balko, Mohammed N. Tantawy, Anna E. Vilgelm, Rizia Bardhan 2020 Vanderbilt University

Multimodal Multiplexed Immunoimaging With Nanostars To Detect Multiple Immunomarkers And Monitor Response To Immunotherapies, Yu-Chuan Ou, Xiaona Wen, Christopher A. Johnson, Daniel Shae, Oscar D. Ayala, Joseph A. Webb, Eugene C. Lin, Rossane C. Delapp, Kelli L. Boyd, Ann Richmond, Anita Mahadevan-Jansen, Marjan Rafat, John T. Wilson, Justin M. Balko, Mohammed N. Tantawy, Anna E. Vilgelm, Rizia Bardhan

Chemical and Biological Engineering Publications

The overexpression of immunomarker programmed cell death protein 1 (PD-1) and engagement of PD-1 to its ligand, PD-L1, are involved in the functional impairment of cluster of differentiation 8+ (CD8+) T cells, contributing to cancer progression. However, heterogeneities in PD-L1 expression and variabilities in biopsy-based assays render current approaches inaccurate in predicting PD-L1 status. Therefore, PD-L1 screening alone is not predictive of patient response to treatment, which motivates us to simultaneously detect multiple immunomarkers engaged in immune modulation. Here, we have developed multimodal probes, immunoactive gold nanostars (IGNs), that accurately detect PD-L1+ tumor cells and CD8+ T cells simultaneously in ...


Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek 2020 Institute of the Chemistry of Plant Substances, Tashkent, Uzbekistan

Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek

CHEMISTRY AND CHEMICAL ENGINEERING

The article discusses use of the insect cell cultures for biotechnological purposes. Optimal conditions for the cultiva-tion of Spodoptera exigua and Lymantria dispar cell lines were selected.


Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich 2020 Institute of Bioorganic Chemistry, Tashkent, Uzbekistan

Development Of A Method Of Oriented Immobilization Of Lipases On A Solid Carrier, Ziyavitdinov Jamolitdin Fazlitdinovich, Inogamov Utkir Kudratillayevich, Berdiev Nodir Sharifovich, Ishimov Uchkun Jomuradovich

CHEMISTRY AND CHEMICAL ENGINEERING

The methods of oriented immobilization of lipases on a solid carrier - Silochrome C-80 have been developed. The resulting sorbents were tested in the process of obtaining biodiesel from cotton oil. It was shown that immobilized lipase on Silochrome C-80 with NH2 group has the highest transetrification ability and stability, while in sorbents with the enzyme immobilized with -COOH and -SH groups this value was 87 and 53%, respectively. Biocatalysis using an affinity sorbent of immobilized lipase with the –NH2 group has a high operational stability, which, after 240 hours of operation, retains more than 50%, and after 480 hours, 17 ...


Obtaining Of The “Green Flourecent Protein - Gfp” In Yeast Of Pichia Pastoris As Repoter Of Heterological Protein Expression, ASHIRОV Оybek, SASMAKОV Sobirdjan, ABDURAKHMANОV Jaloliddin, HASANОV Shuhrat 2020 Institute of the Chemistry of Plant Substances, Tashkent, Uzbekistan

Obtaining Of The “Green Flourecent Protein - Gfp” In Yeast Of Pichia Pastoris As Repoter Of Heterological Protein Expression, Ashirоv Оybek, Sasmakоv Sobirdjan, Abdurakhmanоv Jaloliddin, Hasanоv Shuhrat

CHEMISTRY AND CHEMICAL ENGINEERING

Based on pPIC3.5 and pPIC9 yeast vectors the recombinant plasmid DNA pPIC3.5-GFP (8464 bp) and pPIC9-GFP (8718 bp) containing cDNA (719 bp) of Green fluorescent protein (GFP) were cloned. The obtained plasmids were transformed into yeast cells of the GS115 Pichia pastoris strain. Transformants are selected on histidine-deficient medium. Recombinant clones of pPIC3.5-GFP and pPIC9-GFP were identified by detecting cell fluorescence. GFP expression in yeast is confirmed by green fluorescence. Using electrophoresis in PAAG the molecular weight of the synthesized protein (≈27 kDa) is determined. The resulting reporter constructs and recombinant strains can be used to study ...


Optimization And Validation Of The Neurolux Wireless Optoelectronics System For Optogenetics, Karis Courey, Su Hyun Lee Ph.D., Adam Smith Ph.D., Nicholas Cilz Ph.D., Sarah K. Williams Avram Ph.D., Adi Cymerblit-Sabba Ph.D., June Song, Nicholas Leipzig Ph.D., W. Scott Young M.D., Ph.D. 2020 University of Akron

Optimization And Validation Of The Neurolux Wireless Optoelectronics System For Optogenetics, Karis Courey, Su Hyun Lee Ph.D., Adam Smith Ph.D., Nicholas Cilz Ph.D., Sarah K. Williams Avram Ph.D., Adi Cymerblit-Sabba Ph.D., June Song, Nicholas Leipzig Ph.D., W. Scott Young M.D., Ph.D.

Williams Honors College, Honors Research Projects

Utilizing light and genetic engineering, optogenetics permits the manipulation of events within cells via light using the light-sensitive properties of single-component microbial opsins. Microbial opsins are activated by a light source, such as lasers, light-emitting diodes, and incandescent sources that deliver light to the region of interest either directly or indirectly, such as through fiberoptics. In classical in vivo optogenetics, the wiring of optic fibers necessitates tethering of animals by the optic fiber to the light source. The novel NeuroLux wireless optoelectronic system for optogenetics circumvents issues pertaining to classical optogenetics by utilizing near-field power transfer via magnetic coil antennae ...


Optimization Of E-Textiles For Lactic Acid Detection, Andrew Laboy, Mallory David 2020 The University of Akron

Optimization Of E-Textiles For Lactic Acid Detection, Andrew Laboy, Mallory David

Williams Honors College, Honors Research Projects

The overall goal of the project is to develop an e-textile that will be able to determine different characteristics of an athlete's health through multiple sensors. The goal of these sensors is to monitor physiological parameters for health and fitness uses in real time. Building from the existing sodium concentration sensor, these new sensors will detect lactate levels within sweat to monitor instantaneous health which allows customization of workout and overall health regimen.


Utilizing Virtual High-Throughput Screens To Identify Promising Inhibitors Of Complement Factor C1s, Lyndsey Schmucker 2020 The University of Akron

Utilizing Virtual High-Throughput Screens To Identify Promising Inhibitors Of Complement Factor C1s, Lyndsey Schmucker

Williams Honors College, Honors Research Projects

A large study was conducted to identify possible drug candidates to treat various diseases using computer algorithms, virtual high-throughput screens, and experimental validation of activity. Since the algorithm has the capability to screen millions of compounds, it is beneficial to pharmaceutical development as it allows a larger pool of compounds to be considered that would have otherwise been overlooked. As part of this larger study, this project attempts to identify drug candidates to treat human complement factor C1, a protein which causes tissue damage when underregulated1. A series of designed experiments validate candidates and confirm the performance of the ...


Detection And Thermal Stabilization Of Virus Based On Surface Properties, Xue Mi 2020 Michigan Technological University

Detection And Thermal Stabilization Of Virus Based On Surface Properties, Xue Mi

Dissertations, Master's Theses and Master's Reports

Viral diseases take the lives of millions of people each year. The most effective methods to prevent viral disease outbreak are viral detection to reduce contact with viral pathogens and vaccines to prevent disease. To reduce the costs of the detection of viruses and improve vaccine formulation, we explored viral surface properties. The properties we have focused on are viral hydrophobicity and surface charge using chemical force microscopy (CFM). CFM is a single-particle technique that measures the adhesion force of a functionalized atomic force microscopy (AFM) probe, and in this study, a virus covalently bound to a surface. The non-enveloped ...


Synthesis And Applications Of Theranostic Oligonucleotides Carrying Multiple Fluorine Atoms, Valeriy G. Metelev, Alexei A. Bogdanov 2020 University of Massachusetts Medical School

Synthesis And Applications Of Theranostic Oligonucleotides Carrying Multiple Fluorine Atoms, Valeriy G. Metelev, Alexei A. Bogdanov

Open Access Articles

The use of various oligonucleotide (ON) syntheses and post-synthetic strategies for targeted chemical modification enables improving their efficacy as potent modulators of gene expression levels in eukaryotic cells. However, the search still continues for new approaches designed for increasing internalization, lysosomal escape, and tissue specific delivery of ON. In this review we emphasized all aspects related to the synthesis and properties of ON derivatives carrying multifluorinated (MF) groups. These MF groups have unique physico-chemical properties because of their simultaneous hydrophobicity and lipophobicity. Such unusual combination of properties results in the overall modification of ON mode of interaction with the cells ...


Elucidating The Properties And Mechanism For Cellulose Dissolution In Tetrabutylphosphonium-Based Ionic Liquids Using High Concentrations Of Water, Brad Crawford 2020 West Virginia University

Elucidating The Properties And Mechanism For Cellulose Dissolution In Tetrabutylphosphonium-Based Ionic Liquids Using High Concentrations Of Water, Brad Crawford

Graduate Theses, Dissertations, and Problem Reports

The structural, transport, and thermodynamic properties related to cellulose dissolution by tetrabutylphosphonium chloride (TBPCl) and tetrabutylphosphonium hydroxide (TBPH)-water mixtures have been calculated via molecular dynamics simulations. For both ionic liquid (IL)-water solutions, water veins begin to form between the TBPs interlocking arms at 80 mol % water, opening a pathway for the diffusion of the anions, cations, and water. The water veins allow for a diffusion regime shift in the concentration region from 80 to 92.5 mol % water, providing a higher probability of solvent interaction with the dissolving cellulose strand. The hydrogen bonding was compared between small and ...


A Framework For Heterologous Biosynthesis Of Natural Products In Mammalian Cells Via Polymer-Mediated Transfections, Logan Warriner 2020 University of Kentucky

A Framework For Heterologous Biosynthesis Of Natural Products In Mammalian Cells Via Polymer-Mediated Transfections, Logan Warriner

Theses and Dissertations--Chemical and Materials Engineering

With the promise to treat a multi-faceted list of serious inherited and acquired diseases, such as cancer, neurodegenerative and infectious diseases, and inherited genetic indications, gene therapy has continued to push the boundaries of traditional medicine since its earliest implementation. While much progress has been made, clinical success has largely remained elusive. Immunogenicity, difficulty producing commercially relevant quantities, and having a limited genetic payload still limits the ability of viruses to act as directed delivery agents for genetic material. As such, researchers have turned to cationic synthetic materials as a means of delivering nucleic acids, which can circumvent the immune ...


Synthesis And Characterization Of Rapidly-Degrading Polyanhydrides As Vaccine Adjuvants, Sean M. Kelly, Akash Mitra, Srishti Mathur, Balaji Narasimhan 2019 Iowa State University

Synthesis And Characterization Of Rapidly-Degrading Polyanhydrides As Vaccine Adjuvants, Sean M. Kelly, Akash Mitra, Srishti Mathur, Balaji Narasimhan

Chemical and Biological Engineering Publications

There is a currently a need to develop adjuvants that are best suited to simultaneously enhance immune responses, induce immunologic memory, improve patient compliance (i.e., reduce doses and inflammation), and provide vaccine shelf stability for stockpiling and global deployment to challenging environments. Biodegradable polyanhydrides have been investigated extensively to overcome such challenges. It has been shown that controlling copolymer composition can result in chemistry-dependent immunomodulatory capabilities. These studies have revealed that copolymers rich in sebacic acid (SA) are highly internalized by antigen presenting cells and confer improved shelf stability of encapsulated proteins, while copolymers rich in 1,8-bis(p-carboxyphenoxy ...


Common Secondary And Tertiary Structural Features Of Aptamer–Ligand Interaction Shared By Rna Aptamers With Different Primary Sequences, Muslum Ilgu, Shuting Yan, Ryan M. Khounlo, Monica H. Lamm, Marit Nilsen-Hamilton 2019 Iowa State University, Aptalogic Inc., and Middle East Technical University

Common Secondary And Tertiary Structural Features Of Aptamer–Ligand Interaction Shared By Rna Aptamers With Different Primary Sequences, Muslum Ilgu, Shuting Yan, Ryan M. Khounlo, Monica H. Lamm, Marit Nilsen-Hamilton

Chemical and Biological Engineering Publications

Aptamer selection can yield many oligonucleotides with different sequences and affinities for the target molecule. Here, we have combined computational and experimental approaches to understand if aptamers with different sequences but the same molecular target share structural and dynamical features. NEO1A, with a known NMR-solved structure, displays a flexible loop that interacts differently with individual aminoglycosides, its ligand affinities and specificities are responsive to ionic strength, and it possesses an adenosine in the loop that is critical for high-affinity ligand binding. NEO2A was obtained from the same selection and, although they are only 43% identical in overall sequence, NEO1A and ...


Cdse Quantum Dots Synthesis Laboratory Course For High School Students, Danlin Zuo, Gyuseok Kim, David Jones 2019 Singh Center for Nanotechnology

Cdse Quantum Dots Synthesis Laboratory Course For High School Students, Danlin Zuo, Gyuseok Kim, David Jones

Protocols and Reports

Cadmium selenide quantum dot is a fascinating subject for leading high school students to the quantum world. An 8-hour laboratory course for up to 12 high school students is proposed. The 8-hour course consist of two 4-hours sections. This laboratory course includes the quantum dot syntheses, absorption and emission characterization, and data analysis. The proposes process runs at relatively lower temperature which means safe and easy, and shows apparent experimental results.


Bioluminescence Resonance Energy Transfer (Bret) - Based Nanostructured Biosensor For Detection Of Glucose, Eugene Hwang 2019 The University of Western Ontario

Bioluminescence Resonance Energy Transfer (Bret) - Based Nanostructured Biosensor For Detection Of Glucose, Eugene Hwang

Electronic Thesis and Dissertation Repository

Bioluminescence resonance energy transfer (BRET) is a distance dependent, non-radiative energy transfer, which uses a bioluminescent protein to excite an acceptor through resonance energy transfer. In this thesis, BRET technology is incorporated into a sensor comprised of a recombinant protein and quantum dots. The recombinant protein, which includes the bioluminescent protein, Renilla luciferase (Rluc), is used as the donor molecule and cadmium tellurium quantum dots as the acceptor molecules. Separating the donor-acceptor pair is a recombinant protein, glucose binding protein, which changes conformation upon binding glucose and brings the pair closer together, thus allowing BRET to occur. Optimization of the ...


Effect Of Annealing On The Contact Resistance Of Aluminum On A P-Type Substrate, Shrey Shah, George Patrick Watson 2019 Singh Center for Nanotechnology

Effect Of Annealing On The Contact Resistance Of Aluminum On A P-Type Substrate, Shrey Shah, George Patrick Watson

Protocols and Reports

Aluminum contacts are widely used to form both ohmic and rectifying contacts. The process to form these contacts involves annealing, thus it is important to study the effect of annealing on the electrical properties of the contacts. Here, we present a way to measure the contact resistance of aluminum contacts formed on a p-type silicon substrate. It was found the contact resistivity decreased by an average of 18%. It was thus found that annealing at 400°C in a forming gas environment improves the electrical properties of aluminum contacts.


In-Situ Extraction And Impregnation Of Black Walnut Husk Into Polyethylene Film Using Supercritical Carbon Dioxide With An Ethanol Modifier, Johnathon Wenzel, Veronica Moorman, Lihua Wang, Cheryl S. Samaniego, Michelle Ammerman, Isaiah Spencer-Williams, Mitchell Hall 2019 Kettering University

In-Situ Extraction And Impregnation Of Black Walnut Husk Into Polyethylene Film Using Supercritical Carbon Dioxide With An Ethanol Modifier, Johnathon Wenzel, Veronica Moorman, Lihua Wang, Cheryl S. Samaniego, Michelle Ammerman, Isaiah Spencer-Williams, Mitchell Hall

Chemical Engineering Publications

Walnuts are commonly cultivated for their kernel, which is a rich source of antioxidant phenolic compounds. The husk likewise contains antioxidant and antimicrobial compounds, but is typically discarded without further processing. Antioxidant compounds are useful in creating active packaging films, but typically decompose at melt extrusion temperatures in polymer processing. Due to carbon dioxide's low critical point and ability to swell polymer films, supercritical carbon dioxide may be used to impregnate phenolic compounds into polymers. For this study, a novel technique is used to simultaneously produce walnut husk extracts and impregnate the extract into polymer films in the same ...


Gradient Precipitation Of Recombinant Proteins Contained In Silkworm Larvae (Bombyx Mori) With Ammonium Sulphate, Abdurhakhmanov Jaloliddin, Sasmakov Sobirdjan, Khasanov Shuhrat, Ashirov Оybek 2019 Institute of the Chemistry of Plant Substances, Tashkent, Uzbekistan

Gradient Precipitation Of Recombinant Proteins Contained In Silkworm Larvae (Bombyx Mori) With Ammonium Sulphate, Abdurhakhmanov Jaloliddin, Sasmakov Sobirdjan, Khasanov Shuhrat, Ashirov Оybek

CHEMISTRY AND CHEMICAL ENGINEERING

We investigated the protein extract of silkworm larvae. For the first time, an extract containing proteins from silkworm larvae, obtained by fractional precipitation using ammonium sulfate, was studied. As a result of experiment is established, that solutions of ammonium sulfate with a concentration from 20% to 65% are optimal for the sedimentation of protein molecules with a mass in the range of 25 and 45 kDa. Optimal results of the study proposed to use in the process of purification of recombinant proteins.


Vitamin A Deficiency Impairs The Immune Response To Intranasal Vaccination And Rsv Infection In Neonatal Calves, Jodi L. McGill, Sean M. Kelly, Mariana Guerra-Maupome, Emma Winkley, Jamie Henningson, Balaji Narasimhan, Randy E. Sacco 2019 Iowa State University

Vitamin A Deficiency Impairs The Immune Response To Intranasal Vaccination And Rsv Infection In Neonatal Calves, Jodi L. Mcgill, Sean M. Kelly, Mariana Guerra-Maupome, Emma Winkley, Jamie Henningson, Balaji Narasimhan, Randy E. Sacco

Chemical and Biological Engineering Publications

Respiratory syncytial virus (RSV) infection is a leading cause of severe acute lower respiratory tract infection in infants and children worldwide. Vitamin A deficiency (VAD) is one of the most prevalent nutrition-related health problems in the world and is a significant risk factor in the development of severe respiratory infections in infants and young children. Bovine RSV (BRSV) is a primary cause of lower respiratory tract disease in young cattle. The calf model of BRSV infection is useful to understand the immune response to human RSV infection. We have previously developed an amphiphilic polyanhydride nanoparticle (NP)-based vaccine (i.e ...


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