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Integrated Self-Assembly Of The Mms6 Magnetosome Protein To Form An Iron-Responsive Structure, Shuren Feng, Lijun Wang, Pierre E. Palo, Xunpei Liu, Surya K. Mallapragada, Marit Nilsen-Hamilton 2016 Iowa State University

Integrated Self-Assembly Of The Mms6 Magnetosome Protein To Form An Iron-Responsive Structure, Shuren Feng, Lijun Wang, Pierre E. Palo, Xunpei Liu, Surya K. Mallapragada, Marit Nilsen-Hamilton

Surya K Mallapragada

A common feature of biomineralization proteins is their self-assembly to produce a surface consistent in size with the inorganic crystals that they produce. Mms6, a small protein of 60 amino acids from Magnetospirillum magneticum strain AMB-1 that promotes the in vitro growth of superparamagnetic magnetite nanocrystals, assembles in aqueous solution to form spherical micelles that could be visualized by TEM and AFM. The results reported here are consistent with the view that the N and C-terminal domains interact with each other within one polypeptide chain and across protein units in the assembly. From studies to determine the amino acid residues ...


Self-Assembly And Biphasic Iron-Binding Characteristics Of Mms6, A Bacterial Protein That Promotes The Formation Of Superparamagnetic Magnetite Nanoparticles Of Uniform Size And Shape, Lijun Wang, Tanya Prozorov, Pierre E. Palo, Xunpei Liu, David Vaknin, Ruslan Prozorov, Surya K. Mallapragada, Marit Nilsen-Hamilton 2016 Iowa State University

Self-Assembly And Biphasic Iron-Binding Characteristics Of Mms6, A Bacterial Protein That Promotes The Formation Of Superparamagnetic Magnetite Nanoparticles Of Uniform Size And Shape, Lijun Wang, Tanya Prozorov, Pierre E. Palo, Xunpei Liu, David Vaknin, Ruslan Prozorov, Surya K. Mallapragada, Marit Nilsen-Hamilton

Surya K Mallapragada

Highly ordered mineralized structures created by living organisms are often hierarchical in structure with fundamental structural elements at nanometer scales. Proteins have been found responsible for forming many of these structures, but the mechanisms by which these biomineralization proteins function are generally poorly understood. To better understand its role in biomineralization, the magnetotactic bacterial protein, Mms6, which promotes the formation in vitro of superparamagnetic magnetite nanoparticles of uniform size and shape, was studied for its structure and function. Mms6 is shown to have two phases of iron binding: one high affinity and stoichiometric and the other low affinity, high capacity ...


Effect Of Surface Hydrophobicity On The Function Of The Immobilized Biomineralization Protein Mms6, Xunpei Liu, Honghu Zhang, Srikanth Nayak, German Parada, James W. Anderegg, Shuren Feng, Marit Nilsen-Hamilton, Mufit Akinc, Surya K. Mallapragada 2016 Iowa State University

Effect Of Surface Hydrophobicity On The Function Of The Immobilized Biomineralization Protein Mms6, Xunpei Liu, Honghu Zhang, Srikanth Nayak, German Parada, James W. Anderegg, Shuren Feng, Marit Nilsen-Hamilton, Mufit Akinc, Surya K. Mallapragada

Surya K Mallapragada

Magnetotactic bacteria produce magnetic nanocrystals with uniform shapes and sizes in nature, which has inspired in vitro synthesis of uniformly sized magnetite nanocrystals under mild conditions. Mms6, a biomineralization protein from magnetotactic bacteria with a hydrophobic N-terminal domain and a hydrophilic C-terminal domain, can promote formation of magnetite nanocrystals in vitro with well-defined shape and size in gels under mild conditions. Here we investigate the role of surface hydrophobicity on the ability of Mms6 to template magnetite nanoparticle formation on surfaces. Our results confirmed that Mms6 can form a protein network structure on a monolayer of hydrophobic octadecanethiol (ODT)-coated ...


Self-Assembly And Biphasic Iron-Binding Characteristics Of Mms6, A Bacterial Protein That Promotes The Formation Of Superparamagnetic Magnetite Nanoparticles Of Uniform Size And Shape, Lijun Wang, Tanya Prozorov, Pierre E. Palo, Xunpei Liu, David Vaknin, Ruslan Prozorov, Surya K. Mallapragada, Marit Nilsen-Hamilton 2016 Iowa State University

Self-Assembly And Biphasic Iron-Binding Characteristics Of Mms6, A Bacterial Protein That Promotes The Formation Of Superparamagnetic Magnetite Nanoparticles Of Uniform Size And Shape, Lijun Wang, Tanya Prozorov, Pierre E. Palo, Xunpei Liu, David Vaknin, Ruslan Prozorov, Surya K. Mallapragada, Marit Nilsen-Hamilton

Surya K Mallapragada

Highly ordered mineralized structures created by living organisms are often hierarchical in structure with fundamental structural elements at nanometer scales. Proteins have been found responsible for forming many of these structures, but the mechanisms by which these biomineralization proteins function are generally poorly understood. To better understand its role in biomineralization, the magnetotactic bacterial protein, Mms6, which promotes the formation in vitro of superparamagnetic magnetite nanoparticles of uniform size and shape, was studied for its structure and function. Mms6 is shown to have two phases of iron binding: one high affinity and stoichiometric and the other low affinity, high capacity ...


Biomimetic Self-Assembling Copolymer-Hydroxyapatite Nanocomposites With The Nanocrystal Size Controlled By Citrate, Y.-Y. Hu, X. P. Liu, X. Ma, A. Rawal, Tanya Prozorov, Mufit Akinc, Surya K. Mallapragada, Klaus Schmidt-Rohr 2016 Ames Laboratory

Biomimetic Self-Assembling Copolymer-Hydroxyapatite Nanocomposites With The Nanocrystal Size Controlled By Citrate, Y.-Y. Hu, X. P. Liu, X. Ma, A. Rawal, Tanya Prozorov, Mufit Akinc, Surya K. Mallapragada, Klaus Schmidt-Rohr

Surya K Mallapragada

Citrate binds strongly to the surface of calcium phosphate (apatite) nanocrystals in bone and is thought to prevent crystal thickening. In this work, citrate added as a regulatory element enabled molecular control of the size and stability of hydroxyapatite (HAp) nanocrystals in synthetic nanocomposites, fabricated with self-assembling block copolymer templates. The decrease of the HAp crystal size within the polymer matrix with increasing citrate concentration was documented by solid-state nuclear magnetic resonance (NMR) techniques and wide-angle X-ray diffraction (XRD), while the shapes of HAp nanocrystals were determined by transmission electron microscopy (TEM). Advanced NMR techniques were used to characterize the ...


Effect Of Surface Hydrophobicity On The Function Of The Immobilized Biomineralization Protein Mms6, Xunpei Liu, Honghu Zhang, Srikanth Nayak, German Parada, James W. Anderegg, Shuren Feng, Marit Nilsen-Hamilton, Mufit Akinc, Surya K. Mallapragada 2016 Iowa State University

Effect Of Surface Hydrophobicity On The Function Of The Immobilized Biomineralization Protein Mms6, Xunpei Liu, Honghu Zhang, Srikanth Nayak, German Parada, James W. Anderegg, Shuren Feng, Marit Nilsen-Hamilton, Mufit Akinc, Surya K. Mallapragada

Surya K Mallapragada

Magnetotactic bacteria produce magnetic nanocrystals with uniform shapes and sizes in nature, which has inspired in vitro synthesis of uniformly sized magnetite nanocrystals under mild conditions. Mms6, a biomineralization protein from magnetotactic bacteria with a hydrophobic N-terminal domain and a hydrophilic C-terminal domain, can promote formation of magnetite nanocrystals in vitro with well-defined shape and size in gels under mild conditions. Here we investigate the role of surface hydrophobicity on the ability of Mms6 to template magnetite nanoparticle formation on surfaces. Our results confirmed that Mms6 can form a protein network structure on a monolayer of hydrophobic octadecanethiol (ODT)-coated ...


Integrated Self-Assembly Of The Mms6 Magnetosome Protein To Form An Iron-Responsive Structure, Shuren Feng, Lijun Wang, Pierre E. Palo, Xunpei Liu, Surya K. Mallapragada, Marit Nilsen-Hamilton 2016 Iowa State University

Integrated Self-Assembly Of The Mms6 Magnetosome Protein To Form An Iron-Responsive Structure, Shuren Feng, Lijun Wang, Pierre E. Palo, Xunpei Liu, Surya K. Mallapragada, Marit Nilsen-Hamilton

Surya K Mallapragada

A common feature of biomineralization proteins is their self-assembly to produce a surface consistent in size with the inorganic crystals that they produce. Mms6, a small protein of 60 amino acids from Magnetospirillum magneticum strain AMB-1 that promotes the in vitro growth of superparamagnetic magnetite nanocrystals, assembles in aqueous solution to form spherical micelles that could be visualized by TEM and AFM. The results reported here are consistent with the view that the N and C-terminal domains interact with each other within one polypeptide chain and across protein units in the assembly. From studies to determine the amino acid residues ...


Interfacial Properties And Iron Binding To Bacterial Proteins That Promote The Growth Of Magnetite Nanocrystals: X-Ray Reflectivity And Surface Spectroscopy Studies, Wenjie Wang, Wei Bu, Lijun Wang, Pierre E. Palo, Surya K. Mallapragada, Marit Nilsen-Hamilton, David Vaknin 2016 Iowa State University

Interfacial Properties And Iron Binding To Bacterial Proteins That Promote The Growth Of Magnetite Nanocrystals: X-Ray Reflectivity And Surface Spectroscopy Studies, Wenjie Wang, Wei Bu, Lijun Wang, Pierre E. Palo, Surya K. Mallapragada, Marit Nilsen-Hamilton, David Vaknin

Surya K Mallapragada

Surface sensitive X-ray scattering and spectroscopic studies have been conducted to determine structural properties of Mms6, the protein in Magnetospirillum magneticum AMB-1 that is implicated as promoter of magnetite nanocrystals growth. Surface pressure versus molecular area isotherms indicate Mms6 forms stable monolayers at the aqueous/vapor interface that are strongly affected by ionic conditions of the subphase. Analysis of X-ray reflectivity from the monolayers shows that the protein conformation at the interface depends on surface pressure and on the presence of ions in the solutions, in particular of iron ions and its complexes. X-ray fluorescence at grazing angles of incidence ...


Recovery Of Valuable Chemicals From Agricultural Waste Through Pyrolysis, Mohammad M. Hossain 2016 The University of Western Ontario

Recovery Of Valuable Chemicals From Agricultural Waste Through Pyrolysis, Mohammad M. Hossain

Electronic Thesis and Dissertation Repository

Agricultural crop residues are a source of inexpensive biomass to convert into bioproducts. The recovery of valuable chemicals from plant waste would partly solve the disposal issue and offer a more environmentally friendly alternative to synthetic chemical production. One approach to separate and concentrate valuable chemicals from biomass is pyrolysis using a batch reactor process. A mechanically fluidized reactor (MFR) was developed to pyrolyze biomass from ambient to temperatures near 600 °C, forming gases that are then condensed in an ice-chilled condenser to form a bio-oil. The bio-oil produced by the MFR can be separated within temperature ranges, termed one-dimensional ...


A Study Of Low C/N Ratio Waste Water Treatment Using Fluidized Bed Bioreactor, Kai Li 2016 The University of Western Ontario

A Study Of Low C/N Ratio Waste Water Treatment Using Fluidized Bed Bioreactor, Kai Li

Electronic Thesis and Dissertation Repository

In wastewater treatment field, one of the severe challenges is the handling of ammonia-rich wastewater with a low C/N ratio. The failure of nitrogen treatment can lead to adverse ecological and biological effects including depletion of dissolved oxygen, eutrophication, toxicity to aquatic life, and reduction in the suitability of water for reuse. Conventionally, extra organic carbon dosing is required in treating low C/N wastewater. Alternately, partial nitrification is of interest as an emerging technology for its’ lesser need of organic carbon addition and cost savings in aeration. In this study, a lab-scale partial nitrification fluidized bed bioreactor (PNFBR ...


Polymeric Superparamagnetic Nanoparticles For Drug Delivery Applications, Leena Mohammed 2016 The University of Western Ontario

Polymeric Superparamagnetic Nanoparticles For Drug Delivery Applications, Leena Mohammed

Electronic Thesis and Dissertation Repository

Drug delivery systems based on magnetic nanoparticles present a promising avenue for controlled targeted therapeutics, especially in cancer therapy. Conventional systematic therapeutics encompasses numerous side effects due to its limited selectivity between healthy and cancerous cells. In this thesis, novel polymeric-metallic hybrid nanoaggregates were developed to address this challenge. Magnetite nanoparticles were synthesized via precipitation of iron oxide and was surface modified using a unique chitosan derivative, glycol chitosan (GC), loaded with progesterone for potential hormonal therapy application for breast cancer. Surface characterizations techniques, in vitro drug release kinetics, investigation of progesterone release mechanism by mathematical modeling, and cell cytotoxicity ...


Identification Of Soil Microbes Capable Of Utilizing Cellobiosan, Jieni Lian, Jinlyung Choi, Yee Shiean Tan, Adina C. Howe, Zhiyou Wen, Laura R. Jarboe 2016 Iowa State University

Identification Of Soil Microbes Capable Of Utilizing Cellobiosan, Jieni Lian, Jinlyung Choi, Yee Shiean Tan, Adina C. Howe, Zhiyou Wen, Laura R. Jarboe

Chemical and Biological Engineering Publications

Approximately 100 million tons of anhydrosugars, such as levoglucosan and cellobiosan, are produced through biomass burning every year. These sugars are also produced through fast pyrolysis, the controlled thermal depolymerization of biomass. While the microbial pathways associated with levoglucosan utilization have been characterized, there is little known about cellobiosan utilization. Here we describe the isolation and characterization of six cellobiosan-utilizing microbes from soil samples. Each of these organisms is capable of using both cellobiosan and levoglucosan as sole carbon source, though both minimal and rich media cellobiosan supported significantly higher biomass production than levoglucosan. Ribosomal sequencing was used to identify ...


Combining Metabolic Engineering And Electrocatalysis: Application To The Production Of Polyamides From Sugar, Miguel Suastegui, John E. Matthiesen, Jack M. Carraher, Nacú Hernández, Natalia Rodriguez Quiroz, Adam Okerlund, Eric W. Cochran, Zengyi Shao, Jean-Philippe Tessonnier 2016 Iowa State University

Combining Metabolic Engineering And Electrocatalysis: Application To The Production Of Polyamides From Sugar, Miguel Suastegui, John E. Matthiesen, Jack M. Carraher, Nacú Hernández, Natalia Rodriguez Quiroz, Adam Okerlund, Eric W. Cochran, Zengyi Shao, Jean-Philippe Tessonnier

Chemical and Biological Engineering Publications

Biorefineries aim to convert biomass to a spectrum of products ranging from biofuels to specialty chemicals. To achieve economically sustainable conversion it is crucial to streamline the catalytic and downstream processing steps. Here we report a route that integrates bio- and chemical catalysis to convert glucose into bio-based unsaturated nylon 6,6. An engineered strain of Saccharomyces cerevisiae, with the highest reported muconic acid titer of 559.5 mg L-1 in yeast, was used as the initial biocatalyst to convert glucose into muconic acid. Without any separation, muconic acid was further electrocatalytically hydrogenated to 3-hexenedioic acid with 94% yield, despite ...


Ab Initio Study Of Molecular Interactions In Cellulose Iα, Ajitha Devarajan, Sergiy Markutsya, Monica H. Lamm, Xiaolin Cheng, Jeremy C. Smith, John Ysrael Baluyut, Yana Kholod, Mark S. Gordon, Theresa Lynn Windus 2016 Iowa State University

Ab Initio Study Of Molecular Interactions In Cellulose Iα, Ajitha Devarajan, Sergiy Markutsya, Monica H. Lamm, Xiaolin Cheng, Jeremy C. Smith, John Ysrael Baluyut, Yana Kholod, Mark S. Gordon, Theresa Lynn Windus

Mark S. Gordon

Biomass recalcitrance, the resistance of cellulosic biomass to degradation, is due in part to the stability of the hydrogen bond network and stacking forces between the polysaccharide chains in cellulose microfibers. The fragment molecular orbital (FMO) method at the correlated Møller–Plesset second order perturbation level of theory was used on a model of the crystalline cellulose Iα core with a total of 144 glucose units. These computations show that the intersheet chain interactions are stronger than the intrasheet chain interactions for the crystalline structure, while they are more similar to each other for a relaxed structure. An FMO chain ...


Ab Initio Study Of Molecular Interactions In Cellulose Iα, Ajitha Devarajan, Sergiy Markutsya, Monica H. Lamm, Xiaolin Cheng, Jeremy C. Smith, John Ysrael Baluyut, Yana Kholod, Mark S. Gordon, Theresa Lynn Windus 2016 Iowa State University

Ab Initio Study Of Molecular Interactions In Cellulose Iα, Ajitha Devarajan, Sergiy Markutsya, Monica H. Lamm, Xiaolin Cheng, Jeremy C. Smith, John Ysrael Baluyut, Yana Kholod, Mark S. Gordon, Theresa Lynn Windus

Theresa Windus

Biomass recalcitrance, the resistance of cellulosic biomass to degradation, is due in part to the stability of the hydrogen bond network and stacking forces between the polysaccharide chains in cellulose microfibers. The fragment molecular orbital (FMO) method at the correlated Møller–Plesset second order perturbation level of theory was used on a model of the crystalline cellulose Iα core with a total of 144 glucose units. These computations show that the intersheet chain interactions are stronger than the intrasheet chain interactions for the crystalline structure, while they are more similar to each other for a relaxed structure. An FMO chain ...


The Effect Of Drying On The Release Of Small Hydrophilic Molecular Drugs From Polyelectrolyte Complex Beads, Dounsavanh Letdara 2016 The University of Akron

The Effect Of Drying On The Release Of Small Hydrophilic Molecular Drugs From Polyelectrolyte Complex Beads, Dounsavanh Letdara

Honors Research Projects

The effects of bead composition and dryness on the release rate of small molecules, like a drug or dye, from polyelectrolyte complex (PEC) beads were studied. Release studies were performed on dried and not dried beads prepared using different PEC to drug ratios. The beads were dried for two hours, which allowed them to reach a constant weight. The beads were loaded with a dye, so their release could be detected through the visible light regime. A plate reader was used to determine the absorbance of a sample, and then the amount of dye released was determined. The data presented ...


State Estimation Of Glucose And Insulin Dynamics, Morgan Nicholas Miller 2016 Cleveland State University

State Estimation Of Glucose And Insulin Dynamics, Morgan Nicholas Miller

ETD Archive

Process simulation and state estimation have very important applications in chemical engineering as well as the biomedical field. Diabetes is a rapidly growing disease in the United States with 29 million people already diagnosed. The estimation of glucose and insulin concentration in patients is necessary in order to effectively treat diabetes. The Bergman Minimal Model is a popular process model that is used to simulate glucose and insulin dynamics. A simulation of this model was created based on estimated parameters for the model from historical data. This thesis investigated the estimation of glucose concentration, insulin concentration, and effect of active ...


Molecular Design And Engineering Of Photonic Crystal Hydrogels For Biosensing Applications, Kelsey Irene MacConaghy 2016 University of Colorado Boulder

Molecular Design And Engineering Of Photonic Crystal Hydrogels For Biosensing Applications, Kelsey Irene Macconaghy

Chemical & Biological Engineering Graduate Theses & Dissertations

The rise of personalized medicine, increasing threat of bioterrorism, and growing concern of environmental pollutants necessitates the development of alternative biosensing techniques. Towards this end, we investigated the utility of using chemically and structurally modified photonic hydrogels for optical biosensing applications. Photonic crystal hydrogels are comprised of a crystalline colloidal array polymerized into a stimuli-responsive hydrogel. The crystalline colloidal array is comprised of highly charge nanoparticles that self-assemble into a photonic crystal that Bragg diffracts. The hydrogel is designed to undergo a volume transition in the presence of a target analyte. Altering the hydrogel volume in turn alters the lattice ...


Purification And Recovery Of Infectious Virus Particles Using Osmolyte Flocculation, Ashish Saksule 2016 Michigan Technological University

Purification And Recovery Of Infectious Virus Particles Using Osmolyte Flocculation, Ashish Saksule

Dissertations, Master's Theses and Master's Reports

Viral infectious diseases cause millions of deaths and severe illness all around the world affecting public health and economics. Viral vaccines are helping to fight against viral diseases, but current vaccines are expensive and unavailable, especially in poor and developing countries. When measuring the entire manufacturing processing, the downstream processing of vaccines is the major cost of production. Our goal for this research is to develop a low-cost alternative downstream processing platform for new vaccine manufacturing infrastructures. We have developed a novel osmolyte flocculation method for viral particles. To create a platform purification for several types of viral particles, we ...


Toward The Synthesis And Characterization Of Pb-Doped Zns Nanocrystals For Solar Energy Applications, Allison Durr 2016 Eastern Kentucky University

Toward The Synthesis And Characterization Of Pb-Doped Zns Nanocrystals For Solar Energy Applications, Allison Durr

Online Theses and Dissertations

Efficient and affordable energy conversion and energy storage technologies are required to meet society’s increasing demands. Semiconductor nanocrystals are particularly attractive materials for solar energy conversion applications, as their tunable optoelectronic properties can be manipulated to both optimize the absorbance of solar photons and to afford desirable electronic properties. Further tunability of binary semiconductor nanocrystal systems can be realized through substitutional doping. However, doping can be difficult, as the dopants can cause significant lattice strain in the host crystals. Lead-doped ZnS nanocrystals are one promising material for the conversion of solar photons into storable fuels such as hydrogen gas ...


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