Enhancing Human Schwann Cells Reparative Behavior Using Heparin/Collagen Layer-By-Layer Coatings, 2022 University of Arkansas, Fayetteville
Enhancing Human Schwann Cells Reparative Behavior Using Heparin/Collagen Layer-By-Layer Coatings, Luis Carlos Pinzon-Herrera
Graduate Theses and Dissertations
When a peripheral nerve injury (PNI) occurs, the gold standard for tissue regeneration is the use of autografts. However, due to the secondary effects produced by multiple surgeries involved in the removal and implantation of autografts for very small lesions, it is possible to replace them with the use of Nerve Guide Conduits (NGCs). However, NGCs are limited to short lesions (less than 1 cm). This limitation is caused by the absence of compounds in the extracellular matrix (ECM) that autografts can provide. Since much of the regenerative process takes place on the NGC surface, our work aims to modify …
Life Cycle Assessment Of Novel Electrochemical Struvite Recovery In Municipal Wastewater Treatment At The Plant And U.S. Watershed Scales, 2022 University of Arkansas, Fayetteville
Life Cycle Assessment Of Novel Electrochemical Struvite Recovery In Municipal Wastewater Treatment At The Plant And U.S. Watershed Scales, Karla Morrissey
Graduate Theses and Dissertations
As the supply of phosphorus decreases, nutrient recovery in municipal wastewater treatment has become an important area of research. Recovery methods for phosphorus-containing fertilizers, such as struvite, and other products are currently being developed and assessed for their efficiency and economic feasibility. Potential environmental impacts from nutrient recovery are also being assessed as design for the environment is important, especially with regards to the eutrophying potential of phosphorus in phosphorus-limited freshwater aquatic systems. Life cycle assessment (LCA) is a tool that can be used to estimate potential environmental impacts of a product or service focusing on its entire life cycle. …
Development Of Enzyme-Responsive Polymersomes As A Drug Delivery System For Gm1 Gangliosidosis, 2022 Clemson University
Development Of Enzyme-Responsive Polymersomes As A Drug Delivery System For Gm1 Gangliosidosis, Bipin Chakravarthy Paruchuri
All Dissertations
GM1 gangliosidosis is a lysosomal storage disorder caused by deficiency of β-galactosidase (βgal) and subsequent accumulation of GM1 ganglioside in lysosomes. The rare nature of this disorder presents challenges with prognosis and treatment. Enzyme replacement therapy (ERT) can effectively treat systemic deficiencies, but the intravenously administered enzyme cannot cross the blood-brain barrier (BBB) to treat the central nervous system. A delivery system to encapsulate and deliver the enzyme can facilitate enzyme transport across the BBB with suitable surface modifications. One of the pathological aspects of GM1-affected cells is the upregulation of the lysosomal enzyme hexosaminidase A (HexA). In this dissertation, …
Profiling And Engineering The Unfolded Protein Response In Therapeutic Protein-Producing Cho Cell Lines, 2022 Clemson University
Profiling And Engineering The Unfolded Protein Response In Therapeutic Protein-Producing Cho Cell Lines, Dyllan Rives
All Dissertations
Industrial Chinese hamster ovary (CHO) production of therapeutics requires cell lines with high secretory capacity to avoid an accumulation of improperly folded proteins, or endoplasmic reticulum (ER) stress. This presents a challenging engineering bottleneck. The unfolded protein response (UPR) is initiated to overcome ER stress and reestablish homeostasis. In this dissertation, the impacts of ER stress and the UPR on protein production in mammalian cells are detailed, and both selection- and rational-based strategies for enhancing the ER stress response in productive CHO cell lines are reviewed. This project aims to expand on recent research efforts for engineering ER stress-related responses …
Investigations Of Prokaryotic Defense Systems, 2022 Utah State University
Investigations Of Prokaryotic Defense Systems, Hannah Domgaard
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Bacteria are constantly threatened with infection by mobile genetic elements (MGE) such as bacteriophage and plasmids. Bacteriophage and plasmids require the bacteria's cellular infrastructure to replicate their genomes. Rampant replication can lead to cell death which is one reason why bacteria have developed a diverse array of immune systems to prevent or limit infection. This thesis studies three types of bacterial immune systems, type IV-A CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeat –CRISPR associated), type V-A2 CRISPR-Cas systems, and Wadjet systems.
The type IV-A system lies adjacent to a dinG-like helicase gene. Research has shown that this system can target …
Metabolic Engineering Of Yarrowia Lipolytica For Enhanced Peroxisomal Production Of Polyhydroxyalkanoates, 2022 Clemson University
Metabolic Engineering Of Yarrowia Lipolytica For Enhanced Peroxisomal Production Of Polyhydroxyalkanoates, Michael Spagnuolo
All Dissertations
Traditional chemical processes are made inefficient by the generation of side products and reactions that fail to reach completion. Bioprocesses, on the other hand, lose product potential due to the necessity of growing the organism required to produce the desired compounds. The field of metabolic engineering often seeks to alter this balance between product formation and cell growth to generate more product from the same quantity of feed (reagent). In addition to balance, such organisms may also be engineered to produce the desired products from nontraditional substrates, such as waste compounds. In resource-poor environments, the ability to produce a wanted …
In Vitro Models For The Study Of Liver Biology And Diseases: Advances And Limitations, 2022 Institute of Liver and Biliary Sciences
In Vitro Models For The Study Of Liver Biology And Diseases: Advances And Limitations, Savneet Kaur, Srivatsan Kidambi, Martí Ortega-Ribera, Le Thi Thanh Thuy, Natalia Nieto, Victoria C. Cogger, Wei-Fen Xie, Frank Tacke, Jordi Gracia-Sancho
Department of Chemical and Biomolecular Engineering: Faculty Publications
In vitro models of liver (patho)physiology, new technologies, and experimental approaches are progressing rapidly. Based on cell lines, induced pluripotent stem cells or primary cells derived from mouse or human liver as well as whole tissue (slices), such in vitro single- and multicellular models, including complex microfluidic organ-on-a-chip systems, provide tools to functionally understand mechanisms of liver health and disease. The International Society of Hepatic Sinusoidal Research (ISHSR) commissioned this working group to review the currently available in vitro liver models and describe the advantages and disadvantages of each in the context of evaluating their use for the study of …
Characterizations And Potential Recovery Pathways Of Phosphate Mines Waste Rocks, 2022 Missouri University of Science and Technology
Characterizations And Potential Recovery Pathways Of Phosphate Mines Waste Rocks, Amine El Mahdi Safhi, Hicham Amar, Yahya El Berdai, Mustapha El Ghorfi, Yassine Taha, Rachid Hakkou, Muthanna H. Al-Dahhan, Mostafa Benzaazoua
Chemical and Biochemical Engineering Faculty Research & Creative Works
The Phosphate Ore Production is Steadily Increasing Due to its High Demand for Agriculture, Medicine, and Others. Ore Extraction Generates a Considerable Quantity of Waste Rocks that Are Generally Stocked in Piles. the Current Research Aims to Investigate the Characterization of Phosphate Mine Waste Rocks (PMWR) Generated in Benguerir, Morocco Mine Site. as a Part of a Wide Project Aiming to Recycle Those Stockpiles, Sensor-Based Ore Sorting Will Be Employed to Separate the Different Lithologies. as a Prior Investigation Before Implementing This Technology, Two Samples of 25 Tons from the Destoning and Screening PMWR Piles Were Sampled and Submitted to …
Ab Initio Insight Into The Electrolysis Of Water On Basal And Edge (Fullerene C20) Surfaces Of 4 Å Single-Walled Carbon Nanotubes, 2022 University of Pennsylvania
Ab Initio Insight Into The Electrolysis Of Water On Basal And Edge (Fullerene C20) Surfaces Of 4 Å Single-Walled Carbon Nanotubes, Zhen Jiang, Nadia N. Intan, Qiong Yang
Department of Chemical and Biomolecular Engineering: Faculty Publications
The extreme surface reactivity of 4 Å single-walled carbon nanotubes (SWCNTs) makes for a very promising catalytic material, however, controlling it experimentally has been found to be challenging. Here, we employ ab initio calculations to investigate the extent of surface reactivity and functionalization of 4 Å SWCNTs. We study the kinetics of water dissociation and adsorption on the surface of 4 Å SWCNTs with three different configurations: armchair (3,3), chiral (4,2) and zigzag (5,0). We reveal that out of three different configurations of 4 Å SWCNTs, the surface of tube (5,0) is the most reactive due to its small HOMO–LUMO …
Correlating The Macrostructural Variations Of An Ion Gel With Its Carbon Dioxide Sorption Capacity, 2022 University of Nebraska-Lincoln
Correlating The Macrostructural Variations Of An Ion Gel With Its Carbon Dioxide Sorption Capacity, Tung Nguyen,, Mona Bavarian, Siamak Nejati
Department of Chemical and Biomolecular Engineering: Faculty Publications
We report on a direct correlation between the macroscale structural variations and the gas sorption capacities of an ion gel. Here, we chose 1-ethyl-3-methylimidazolium bis(trifluoromethyl sulfonyl)imide ([Emim][TF2N]) and poly(vinylidene fluoride)-co-hexafluoropropylene (PVDF-HFP) as the ionic liquid and host polymer, respectively. The CO2 sorption in the thin films of the IL-polymer was measured using the gravimetric method. The results of our experiment showed that the trend in CO2 uptake of these mixtures was nonlinearly correlated with the content of IL. Here, we highlight that the variations in the molecular structure of the polymers were the main reason behind …
Frontiers In The Self-Assembly Of Charged Macromolecules, 2022 University of Massachusetts Amherst
Frontiers In The Self-Assembly Of Charged Macromolecules, Khatcher O. Margossian
Doctoral Dissertations
The self-assembly of charged macromolecules forms the basis of all life on earth. From the synthesis and replication of nucleic acids, to the association of DNA to chromatin, to the targeting of RNA to various cellular compartments, to the astonishingly consistent folding of proteins, all life depends on the physics of the organization and dynamics of charged polymers. In this dissertation, I address several of the newest challenges in the assembly of these types of materials. First, I describe the exciting new physics of the complexation between polyzwitterions and polyelectrolytes. These materials open new questions and possibilities within the context …
Electrophilic Aldehyde 4-Hydroxy-2-Nonenal Mediated Signaling And Mitochondrial Dysfunction, 2022 Louisiana State University Health Sciences Center
Electrophilic Aldehyde 4-Hydroxy-2-Nonenal Mediated Signaling And Mitochondrial Dysfunction, Sudha Sharma, Papori Sharma, Tara Bailey, Susmita Bhattarai, Utsab Subedi, Chloe Miller, Hosne Ara, Srivatsan Kidambi, Hong Sun, Manikandan Panchatcharam, Sumitra Miriyala
Department of Chemical and Biomolecular Engineering: Faculty Publications
Reactive oxygen species (ROS), a by-product of aerobic life, are highly reactive molecules with unpaired electrons. The excess of ROS leads to oxidative stress, instigating the peroxidation of polyunsaturated fatty acids (PUFA) in the lipid membrane through a free radical chain reaction and the formation of the most bioactive aldehyde, known as 4-hydroxynonenal (4-HNE). 4-HNE functions as a signaling molecule and toxic product and acts mainly by forming covalent adducts with nucleophilic functional groups in proteins, nucleic acids, and lipids. The mitochondria have been implicated as a site for 4-HNE generation and adduction. Several studies clarified how 4-HNE affects the …
Fabricating 3-Dimensional Human Brown Adipose Microtissues For Transplantation Studies, 2022 University of Nebraska-Lincoln
Fabricating 3-Dimensional Human Brown Adipose Microtissues For Transplantation Studies, Ou Wang, Li Han, Haishuang Lin, Mingmei Tian, Shuyang Zhang, Bin Duan, Soonkyu Chung, Chi Zhang, Xiaojun Lian, Yong Wang, Yuguo Lei
Department of Chemical and Biomolecular Engineering: Faculty Publications
Transplanting cell cultured brown adipocytes (BAs) represents a promising approach to prevent and treat obesity (OB) and its associated metabolic disorders, including type 2 diabetes mellitus (T2DM). However, transplanted BAs have a very low survival rate in vivo. The enzymatic dissociation during the harvest of fully differentiated BAs also loses significant cells. There is a critical need for novel methods that can avoid cell death during cell preparation, transplantation, and in vivo. Here, we reported that preparing BAs as injectable microtissues could overcome the problem. We found that 3D culture promoted BA differentiation and UCP-1 expression, and the optimal initial …
Prototype Catalytic Membrane Reactor For Dimethyl Ether Synthesis Via Co2hydrogenation, 2022 Missouri University of Science and Technology
Prototype Catalytic Membrane Reactor For Dimethyl Ether Synthesis Via Co2hydrogenation, Qiaobei Dong, Weiwei L. Xu, Xiao Fan, Huazheng Li, Naomi Klinghoffer, Travis Pyrzynski, Howard S. Meyer, Xinhua Liang, Miao Yu, Shiguang Li
Chemical and Biochemical Engineering Faculty Research & Creative Works
Dimethyl ether (DME) has become attractive as a potential environmentally friendly substitute for diesel and liquefied petroleum gas (LPG) due to its similar properties to those of LPG, high cetane number, but less carbon emissions. In this work, we developed a novel prototype-scale catalytic membrane reactor to synthesize DME directly from CO2and renewable H2, which could address the environmental and fuel security issues in a cost-effective way. This membrane reactor was equipped with superior hydrophilic NaA zeolite membranes and bifunctional Cu-ZnO-ZrO2-Al2O3/HZSM-5 catalysts. The effects of the reaction temperature and gas …
Reduced Building Energy Consumption By Combined Indoor Co2 And H2o Composition Control, 2022 Missouri University of Science and Technology
Reduced Building Energy Consumption By Combined Indoor Co2 And H2o Composition Control, Anshuman Sinha, Harshul Thakkar, Fateme Rezaei, Yoshiaki Kawajiri, Matthew J. Realff
Chemical and Biochemical Engineering Faculty Research & Creative Works
Rapid growth in global energy consumption has raised concern on the environmental impacts such as ozone layer depletion and climate change. Enclosed space, such as commercial buildings, accounts for about 40% of global energy consumption and the demand is constantly increasing due to increasing population, urbanization, and economic development. The energy demands in the building sector calls for strategic measures to develop energy efficient technologies. This paper presents a strategy to decrease energy demands inside buildings by proposing a ventilation system which regulates the enclosed air quality resulting in reduced air conditioning. The system consists of multiple adsorption beds with …
Atomic Layer Deposited Pt/Tio2-Sio2 And Pt/Zro2-Sio2 For Sequential Adsorption And Oxidation Of Vocs, 2022 Missouri University of Science and Technology
Atomic Layer Deposited Pt/Tio2-Sio2 And Pt/Zro2-Sio2 For Sequential Adsorption And Oxidation Of Vocs, Busuyi O. Adebayo, Han Yu, Ali A. Rownaghi, Xinhua Liang, Fateme Rezaei
Chemical and Biochemical Engineering Faculty Research & Creative Works
In this work, Pt nanoparticles were loaded on SiO2, TiO2-thin-film-modified SiO2 (TiO2-SiO2), or ZrO2-thin-film-modified SiO2 (ZrO2-SiO2) particles and the composites were investigated for sequential adsorption and desorption/catalytic oxidation of benzene. The SiO2 was prepared via sol–gel method, while TiO2-SiO2 and ZrO2-SiO2 were synthesized via atomic layer deposition (ALD) thin film coating of TiO2 or ZrO2 on SiO2 particles substrate. In the sequential capture-reaction tests, the materials were first exposed to ca. 500 ppmv benzene …
Ionic Liquid Pilocarpine Analog As An Antiglaucoma Drug Candidate, 2022 Missouri University of Science and Technology
Ionic Liquid Pilocarpine Analog As An Antiglaucoma Drug Candidate, Juan Wang, Boxuan Li, Jing Tang, Li Qiu, Xin Qiao, Na Xu, Hu Yang
Chemical and Biochemical Engineering Faculty Research & Creative Works
Ionic liquid pilocarpine analog [Pilo-OEG]Cl, the first-of-its-kind ionic liquid antiglaucoma drug candidate, is synthesized and reported. To synthesize [Pilo-OEG]Cl, pilocarpine was reacted with the oligo-polyethylene glycol chloride, 2-[2-(2-chloroethoxy)ethoxy] ethanol, to form an ionic liquid molecule with an imidazole cation and a chloride anion. The chemical structure of [Pilo-OEG]Cl was confirmed with 1H NMR spectroscopy. Compared with pilocarpine (Pilo) and pilocarpine hydrochloride (PiloHCl), [Pilo-OEG]Cl has improved structural stability according to pH measurements and LC-MS analysis. The corneal permeability coefficient of [Pilo-OEG]Cl is 2-fold higher than that of Pilo and 8-fold higher than that of PiloHCl. [Pilo-OEG]Cl does not show apparent toxicity …
Teb 406 Reflection, 2022 Western University
Teb 406 Reflection, Ryan Alfonso Falcon
Undergraduate Student Research Internships Conference
Slides for a non-exhaustive reflection on my work in summer
Improving The Crystal Habit Of Rufinamide, 2022 Western University
Improving The Crystal Habit Of Rufinamide, Peter M. Sadaka
Undergraduate Student Research Internships Conference
This research was developed by utilizing the knowledge gathered and learned about crystallization through my USRI project. The goal was to improve the crystal habit of a selected drug from the many that were available in the lab. To choose this, we looked at all of them and chose the drug with the worst habit and then began working. It was created by organizing my findings for each week and then making it all much more concise for the form of a simple presentation that most could understand.
Nonordered Dendritic Mesoporous Silica Nanoparticles As Promising Platforms For Advanced Methods Of Diagnosis And Therapies, 2022 University of Manchester
Nonordered Dendritic Mesoporous Silica Nanoparticles As Promising Platforms For Advanced Methods Of Diagnosis And Therapies, S. Malekmohammadi, Riaz Ur Rehman Mohammed, H. Samadian, A. Zarebkohan, A. García-Fernández, G.R. Kokil, F. Sharifi, J. Esmaeili, M. Bhia, M. Razavi, M. Bodaghi, T. Kumeria, R. Martínez-Máñez
Department of Chemical and Biomolecular Engineering: Faculty Publications
Dendritic mesoporous silica nanoparticles (DMSNs) are a new generation of porous materials that have gained great attention compared to other mesoporous silicas due to attractive properties, including straightforward synthesis methods, modular surface chemistry, high surface area, tunable pore size, chemical inertness, particle size distribution, excellent biocompatibility, biodegradability, and high pore volume compared with conventional mesoporous materials. The last years have witnessed a blooming growth of the extensive utilization of DMSNs as an efficient platform in a broad spectrum of biomedical and industrial applications, such as catalysis, energy harvesting, biosensing, drug/gene delivery, imaging, theranostics, and tissue engineering. DMSNs are considered great …