Studies On Nanocomposite Coating Produced By Laser-Assisted Process To Prevent Silicone Hydrogels From Bio-Fouling, 2016 The University of Western Ontario
Studies On Nanocomposite Coating Produced By Laser-Assisted Process To Prevent Silicone Hydrogels From Bio-Fouling, Vishnuvardhana Wuppaladhodi
Electronic Thesis and Dissertation Repository
In this thesis, silver nanoparticles incorporated into polyvinylpyrrolidone (PVP) were deposited on silicone hydrogel to improve the hydrophilicity of the silicone hydrogel and prevent the growth of bacteria. Two different processes were employed to produce Ag nanoparticles: (1) Process-A is a photochemical reduction; (2) Process-B is laser ablation in liquid. Following that, MAPLE process was employed to deposit the Ag-PVP nanocomposites on the surface of silicone hydrogel. A solid-state pulsed laser (Nd:YAG) with a wavelength of 532 nm at a fluence of 50.4 mJ/cm2 was used in the MAPLE system to deposit Ag-PVP nanocomposite coating. Our ...
Microbial Nanoculture As An Artificial Microniche, 2016 University of Pennsylvania
Microbial Nanoculture As An Artificial Microniche, Tagbo H. R Niepa, Likai Hou, Hongyuan Jiang, Mark Goulian, Hyun Koo, Kathleen J. Stebe, Daeyeon Lee
Departmental Papers (CBE)
Microbes self-organize in microcolonies while transitioning to a sessile form within a protective biofilm matrix. To enable the detailed study of microbial dynamics within these microcolonies, new sessile culture systems are needed that sequester cells and mimic their complex growth conditions and interactions. We present a new nanoliter-scale sessile culture system that is easily implemented via microfluidics-enabled fabrication. Hundreds of thousands of these nanocultures can be easily generated and imaged using conventional or confocal microscopy. Each nanoculture begins as a several nanoliter droplet of suspended cells, encapsulated by a polydimethylsiloxane (PDMS) membrane. The PDMS shell provides long-lasting mechanical support, enabling ...
Directed Self-Assembly Of Block Copolymer, No1, 2016 Singh Center
Directed Self-Assembly Of Block Copolymer, No1, Hiromichi Yamamoto
Protocols and Reports
The PS-rich and neutral PS-b-PMMA block copolymer (BCP) films were spin coated on the neutral random copolymer hydroxyl-terminated PS-r-PMMA layers grafted on the native oxide and 50 nm thick PECVD amorphous silicon oxide layers. Relationship between the grafting density of BCP and surface density of hydroxyl moiety on silicon oxide is discussed. Furthermore, optimization of annealing BCP films is reported, and wetted and de-wetted BCP films are shown in optical microscope images. In addition, finger print and nanopore structures of BCP films are also indicated in SEM images.
Fermentative Processes Requiring Low Solubility Feed Gases: An Investigation Into Gas-Dependent Microorganisms, 2016 The University of Western Ontario
Fermentative Processes Requiring Low Solubility Feed Gases: An Investigation Into Gas-Dependent Microorganisms, Eric W. Doerr
Electronic Thesis and Dissertation Repository
Two bioprocesses were separately investigated based on their common interest of using gaseous substrates that have low solubility. The first process involved the development of a mixed culture using two organisms capable of utilizing glycerol and carbon monoxide separately to increase biobutanol production, while the second process involved an investigation of different production media used in aerobic xanthan production of Xanthomonas campestris with pressurization effects. It was determined that Clostridium pasteurianum should be used with an organism like Clostridium carboxidivorans or Eubacterium limosum in order for butyrate uptake at 3 g/L to occur with a minimum 0.1 g ...
Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, 2016 University of Nebraska - Lincoln
Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin
Biological Systems Engineering--Dissertations, Theses, and Student Research
The establishment of in vitro culture models utilizes tissue engineering principles to design functional mimics of in vivo environments in vitro. Advantages for the use of in vitro culture models include ethical alleviation of animal models for therapeutic testing, cost efficiency, and a greater ability to study specific mechanisms via a systematic, ground-up approach to development. In this thesis, alginate hydrogels are utilized in the development of in vitro culture models of porcine embryo elongation and growth plate cartilage development. First, the effect of scaffold and modifications to the scaffold were explored in both projects. In order to modulate cell-scaffold ...
Tailoring Zsm-5 Zeolites For The Fast Pyrolysis Of Biomass To Aromatic Hydrocarbons, 2016 Iowa State University
Tailoring Zsm-5 Zeolites For The Fast Pyrolysis Of Biomass To Aromatic Hydrocarbons, Thomas W. Hoff, David W. Gardner, Rajeeva Thilakar, Kaige Wang, Thomas W. Hansen, Robert C. Brown, Jean-Philippe Tessonnier
Chemical and Biological Engineering Publications
The production of aromatic hydrocarbons from cellulose by zeolite-catalyzed fast pyrolysis involves a complex reaction network sensitive to the zeolite structure, crystallinity, elemental composition, porosity, and acidity. The interplay of these parameters under the reaction conditions represents a major roadblock that has hampered significant improvement in catalyst design for over a decade. Here, we studied commercial and laboratory-synthesized ZSM-5 zeolites and combined data from 10 complementary characterization techniques in an attempt to identify parameters common to high-performance catalysts. Crystallinity and framework aluminum site accessibility were found to be critical to achieve high aromatic yields. These findings enabled us to synthesize ...
In Silico Driven Metabolic Engineering Towards Enhancing Biofuel And Biochemical Production, 2016 University of Tennessee - Knoxville
In Silico Driven Metabolic Engineering Towards Enhancing Biofuel And Biochemical Production, Richard Adam Thompson
The development of a secure and sustainable energy economy is likely to require the production of fuels and commodity chemicals in a renewable manner. There has been renewed interest in biological commodity chemical production recently, in particular focusing on non-edible feedstocks. The fields of metabolic engineering and synthetic biology have arisen in the past 20 years to address the challenge of chemical production from biological feedstocks. Metabolic modeling is a powerful tool for studying the metabolism of an organism and predicting the effects of metabolic engineering strategies. Various techniques have been developed for modeling cellular metabolism, with the underlying principle ...
Model Validation And Transition To Turbulence In Orbiting Culture Dishes., 2016 University of Louisville
Model Validation And Transition To Turbulence In Orbiting Culture Dishes., Jonathan Michael D. Thomas
Electronic Theses and Dissertations
Wall shear stress (WSS) is commonly accepted as the primary influence affecting characteristics of anchored endothelial cells when subjected to fluid flow. Orbital shakers are commonly used to study cellular responses due to their ease of use, ability to run several experiments simultaneously, and since they exert physiologically relevant oscillatory shear. These studies require comprehensive resolution of WSS, however the fluid dynamics inside orbiting culture dishes has not yet been well described since the flow is complex and difficult to quantify analytically. A computational fluid dynamics (CFD) model of flow in an orbiting dish has been developed that yields detailed ...
Investigating The Modulation Of Aggregating Amyloid Beta 40, 2016 University of Arkansas, Fayetteville
Investigating The Modulation Of Aggregating Amyloid Beta 40, Marlee J. Motes
Chemical Engineering Undergraduate Honors Theses
Amyloid beta protein has been linked to the formation of Alzheimer’s disease in patients.¹ Plaques form from amyloid beta fibrils. The formation of these plaques between neural connections in the brain are associated with Alzheimer’s disease.² The reduction of the formation of fibrils can be linked to utilizing protein mimics. The protocols that are used to reproduce the simulation of amyloid beta in the brain can be very important. Also, the structure of the protein mimic that is being used to inhibit the formation of fibrils can determine how the amyloid beta plaques are reduced.
Development Of A Simple Handheld Biosensor For Waterborne Pathogens, 2016 University of Arkansas, Fayetteville
Development Of A Simple Handheld Biosensor For Waterborne Pathogens, Bryce C. Jones
Chemical Engineering Undergraduate Honors Theses
Current methods of detecting waterborne pathogens involve testing strips which take 24-48 hours to yield results, or require expensive equipment in order to function. In minimalist environments, these two technologies are not always applicable to test water quality. With the emergence of a new method of PCR, named LAMP PCR, it is possible to quickly and accurately detect pathogen DNA in a water sample. In order to scale this technique into a simple device, the aspects of the reaction must be accommodated, and a visual detection method chosen. A handheld device which keeps the isothermal LAMP PCR stable for the ...
Cloning, Fed-Batch Expression And Purification Of A Novel Anti-Candida Peptide And Development Of A Cleavage Resistant Variant Of Green Fluorescence Protein, 2016 University of Arkansas, Fayetteville
Cloning, Fed-Batch Expression And Purification Of A Novel Anti-Candida Peptide And Development Of A Cleavage Resistant Variant Of Green Fluorescence Protein, Rudra Palash Mukherjee
Theses and Dissertations
This work illustrates that the intelligent design of a bioprocess for the production of peptides of various length is possible by having a prior knowledge of desired product, cell line, fermentation conditions, and choices for downstream recovery. Cloning, expression, and recovery of a novel 12-mer anti-Candida peptide served as an illustrative case for a proposed Escherichia coli platform. The antifungal peptide, expressed as a fusion to GFPUV during high call density fed-batch, was recovered from cell lysate, concentrated using ion exchange chromatography, and finally cleaved with cyanogen bromide. The platform was capable of producing active peptide capable of arresting the ...
Local Delivery Of Ctla-4 Blockade Inhibits Growth Of Pancreatic Tumors, 2016 University of Arkansas, Fayetteville
Local Delivery Of Ctla-4 Blockade Inhibits Growth Of Pancreatic Tumors, Jack Baltz
Biomedical Engineering Undergraduate Honors Theses
Immune checkpoint blockade has demonstrated great potential in activating antitumor immunity. Ipilimumab is a monoclonal antibody which targets cytotoxic T-lymphocyte antigen-4. CTLA-4 belongs to the CD28 class of receptors and is found on the surface of CD4+ and CD8+ T cells. CTLA-4 acts to suppress the immune system when bound to CD80 and CD86 receptors on antigen presenting cells. Ipilimumab, or anti-CTLA-4, has shown to be effective in significantly extending the survival of patients with metastatic melanoma. However, systemic delivery of Ipilimumab also induces significant side effects such as: colitis, dermatitis, uveitis, and hypophysitis. In order to minimize toxicity, we ...
The Development Of A Virtual Sensor In Glucose Monitoring For Non-Insulin Dependent People, 2016 Iowa State University
The Development Of A Virtual Sensor In Glucose Monitoring For Non-Insulin Dependent People, Sophia Masters, Sarah Jacobson
Honors Projects and Posters
The project involves an Android application that acts as a continuous-time monitor of glucose levels for people with Type II diabetes. Our objective was to get information from a glucose lancet meter to automatically upload via Bluetooth into the application. This allows a person to easily monitor their insulin levels without manually inputting glucose levels into the application. The glucose meter used was a ForaCare Test N’ Go system. To aid in the Bluetooth process, we were granted open access to the glucose meter from Fora Care. The process involved using the programming language of Java in the Android software ...
Biomolecular Interactions Control The Shape Of Stains From Drying Droplets Of Complex Fluids, 2016 University of Arizona
Biomolecular Interactions Control The Shape Of Stains From Drying Droplets Of Complex Fluids, Cedric Hurth, Ranjeesh Bhardwaj, Sahar Andalib, Christophe Frankiewicz, Andrew Dobos, Daniel Attinger, Frederic Zenhausern
When a sessile droplet of a complex fluid dries, a stain forms on the solid surface. The structure and pattern of the stain can be used to detect the presence of a specific chemical compound in the sessile droplet. In the present work, we investigate what parameters of the stain or its formation can be used to characterize the specific interaction between an aqueous dispersion of beads and its receptor immobilized on the surface. We use the biotin-streptavidin system as an experimental model. Clear dissimilarities were observed in the drying sequences on streptavidin-coated substrates of droplets of aqueous solutions containing ...
Minor Whey Protein Purification Using Ion-Exchange Column Chromatography, 2016 The University of Western Ontario
Minor Whey Protein Purification Using Ion-Exchange Column Chromatography, Naeimeh Faraji Dizaji
Electronic Thesis and Dissertation Repository
This thesis is concerned with application of mechanistic models for recovery and purification of two minor milk proteins to develop an efficient and robust process. A fundamental and quantitative understanding of the underlying mechanisms assists to evaluate chances and challenges in non-linear chromatography.
The first chapter considers adsorption isotherm data of two minor whey proteins on cation exchanger under various conditions and used as the basis to develop a predictive approach for correlating adsorption behavior using a mechanistic isotherm model. The SMA isotherm model explicitly considers the contributions of protein-adsorbent and protein-protein interactions in the simulation of salt gradients in ...
Sustained Cell Differentiation Of 2d H9 Human Embryonic Stem Cells Into Mesenchymal Stem Cells, 2016 University of Nebraska-Lincoln
Sustained Cell Differentiation Of 2d H9 Human Embryonic Stem Cells Into Mesenchymal Stem Cells, Hannah M. Christian
UCARE Research Products
This experiment consisted of the controlled differentiation of H9 embryonic stem cells to mesenchymal stem cells. Though this experiment was repeated twice and improvement was seen in these repetitions, the cells were only able to be partially differentiated. However, the morphology of the differentiated cells is similar to those of healthy adult mesenchymal stem cells.
The progression of the differentiation can be seen in the microscope slide photos below. Throughout the differentiation, there occurred a decrease in cell survival and reduction of cell growth, but an increase in mesenchymal stem cell morphology. Throughout the last week of the differentiation, very ...
Effect Of Packaging Material On Color Properties Of Catsup Tomato Sauce, 2016 Department of Food Health and Safety, School of Health, Qazvin University of Medical Sciences, Qazvin, Iran
Effect Of Packaging Material On Color Properties Of Catsup Tomato Sauce, Naser Kohan-Nia, Babak Pakbin, Razzagh Mahmoudi, Omid Fakhri
Journal of Applied Packaging Research
Catsup and tomato sauce products currently are one of the most popular and commercially available table sauces particularly consumed by young people and families around the world in recent decades. In this study colorimetric properties of catsup sauce samples including L*, a* and b* values in different packaging materials including PP, PE, PET, Glass and Sachet have been investigated during and after 180 days storage time at 22̊ C (environmental temperature). Hunter Lab system has been used to evaluate the color values of sauce samples. According to obtained results of experimental measurements and sensory evaluations, PP and PE packaging materials ...
Brain Tumor In A Dish: Glioma/Astrocyte Co-Cultures As A Model For In Vitro Studies, 2016 University of Nebraska-Lincoln
Brain Tumor In A Dish: Glioma/Astrocyte Co-Cultures As A Model For In Vitro Studies, Erin Eickman, Christina Wilson, Srivatsan Kidambi
UCARE Research Products
This study seeks to engineer an in vitro co-culture model to elucidate the role of glioma-astrocyte interactions on molecular changes in the tumor microenvironment. The use of patterned co-cultures created with polyelectrolyte multilayers and micromolding in capillaries will allow tthe investigation of cell-cell communication. This study will lead to better understanding of the role of healthy cells in cancer progression and potential treatment options.
Recycling Of Neodymium And Dysprosium From Permanent Magnets, 2016 University of Pennsylvania
Recycling Of Neodymium And Dysprosium From Permanent Magnets, Alan X. Dai, Connor A. Lippincott, Michael E. Nissan, Richard Shim
Senior Design Reports (CBE)
This project seeks to recycle neodymium and dysprosium from used neodymium-iron-boron, NdFeB, permanent magnets. Our alternative recycling strategy is based on a method developed by Dr. Eric Schelter of the University of Pennsylvania Department of Chemistry and his research group. This process involves the use of a novel tripodal nitroxide ligand, H3TriNOX. The proposed process design converts used NdFeB magnets to neodymium and dysprosium. The goal production of neodymium oxide is 126,000 kg/year and of dysprosium oxide is 14,000 kg/year, which would cost $12,500,000 with the current price rate of neodymium and ...
Large-Scale Fermentation Of E. Coli For The Production Of High-Purity Isoprene, 2016 University of Pennsylvania
Large-Scale Fermentation Of E. Coli For The Production Of High-Purity Isoprene, Phillip A. Taylor, Yuta Inaba, Ian J. Pinto
Senior Design Reports (CBE)
We present a process for the production of isoprene via the fermentation of glucose. Based on our current specifications, we conclude that the use of recombinant E.coli for the fermentation of glucose is a novel yet unprofitable venture. Our current design entails the continuous production of isoprene using 3 pre-seed, 3 seed, and 5 production fermenters each with a production fermentation time of 72 hours. Our scheduling of the fermenters allowed us to produce isoprene continuously at a steady rate, and the liquid by-products of the fermentation were removed and sterilized at the end of each batch. Isoprene was ...