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Biochemical and Biomolecular Engineering Commons

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Food Waste To Bio-Products, Elizabeth Handen, Mauricio Diaz Padilla, Hannah Rears, Lyle Rodgers 2017 University of Pennsylvania

Food Waste To Bio-Products, Elizabeth Handen, Mauricio Diaz Padilla, Hannah Rears, Lyle Rodgers

Senior Design Reports (CBE)

The goal of this project was to design and evaluate a project for the collection and processing of food waste and spent oil in Philadelphia. The project was designed to handle 5% of the total commercial waste generated in Philadelphia. This amounted to approximately 9,700 tons/year of food waste and 73,000 gallons/year of spent oil. The process was designed to utilize a BIOFerm™ Dry Fermentation Digestion System. Following the digestion, the biogas produced is passed through a Caterpillar CG132-12 Generator Set, producing electricity to be sold back to the local grid. The digestate from the anaerobic ...


Coffee To Biofuels, Chelsea Giller, Bhavish Malkani, Josh Parasar 2017 University of Pennsylvania

Coffee To Biofuels, Chelsea Giller, Bhavish Malkani, Josh Parasar

Senior Design Reports (CBE)

This project proposes a production plant that will utilize 9.58 million kg/year of spent coffee grounds in order to produce 1.03 million liters/year of coffee biofuel and 2.56 million kg/year of biomass pellets. This plant will be located in northern New Jersey, where the maximum amount of spent coffee grounds can be obtained every day from each of the five boroughs in New York City. Practically, trucks could obtain grounds from 875 Starbucks and Dunkin Donuts coffee shops, collected 3-4 times per week. Overall uptime is taken to be 90%. The biodiesel is formed ...


Low-Cost, Commercial Scale Production Of Sofosbuvir, Joseph Solomon, Jake Hsu, Cynthia Tong, Ryan Bliss 2017 University of Pennsylvania

Low-Cost, Commercial Scale Production Of Sofosbuvir, Joseph Solomon, Jake Hsu, Cynthia Tong, Ryan Bliss

Senior Design Reports (CBE)

Recent advances in antiviral therapeutics have produced highly effective small molecule drugs to treat Hepatitis C, a deadly infection of the liver. Sofosbuvir, a hepatitis C drug developed by Gilead Sciences, is a breakthrough treatment due to its low side effects and high cure rate. However, the cost of treatment is extraordinarily high, priced at $84,000 per treatment in the US. In response to backlash regarding the cost barriers in developing countries, Gilead has reached licensing agreements with generic pharmaceutical companies to produce the drug for markets in low-income countries such as India, Kenya, and Cuba among others. The ...


Direct Route To Phenol From Benzene, Bryan Daowdat, Gerard D. Hoeltzel, Robert Tannenbaum 2017 University of Pennsylvania

Direct Route To Phenol From Benzene, Bryan Daowdat, Gerard D. Hoeltzel, Robert Tannenbaum

Senior Design Reports (CBE)

Phenol production, expected to exceed 13MM metric tons in 2017, is a significant global industry with many flaws in its current manufacturing method. The Hock process essentially converts high value propylene to low value acetone. The proposed process design, detailed in this report, provides an alternative reaction pathway that utilizes a direct synthesis from benzene, as developed at the Council of Scientific and Industrial Research (CSIR) in New Delhi, India. The endorsed proposal is in accordance with US Patent 8,772,552 B2, that describes the production of phenol via vapor phase oxidation of benzene over a copper chromium oxide ...


Optimization Of Oleosin 30g Production For Echocardiography, Annette Krysiewicz, Andrew Parsons, Sergio Rodriguez-Labra 2017 University of Pennsylvania - Biostatistics

Optimization Of Oleosin 30g Production For Echocardiography, Annette Krysiewicz, Andrew Parsons, Sergio Rodriguez-Labra

Senior Design Reports (CBE)

Provided they are uniform in size, monodisperse microbubbles behave as contrast agents to enhance echocardiographic imaging. Compounds like Oleosin 30G with surfactant-like properties help stabilize microbubbles - thereby ensuring their uniform size. Designed herein is an industrial-scale plant to produce medical-grade Oleosin 30G with a process consisting of three steps: 1) upstream production via recombinant E. coli in an integrated batch bioprocessing model, 2) downstream purification, and 3) processing by microfluidic manifolds. Ultimately Oleosin 30G-coated microbubbles are manufactured, ready for injection within one month. Owing to its unique properties and cost-effective production, Oleosin 30G has the potential to outcompete current market ...


The Effects Of Adaptogens On The Physical And Psychological Symptoms Of Chronic Stress, Tosin Ajala 2017 Georgia State University

The Effects Of Adaptogens On The Physical And Psychological Symptoms Of Chronic Stress, Tosin Ajala

Georgia State Undergraduate Research Conference

No abstract provided.


Genetic Code Expansion In Biochemical Investigations And Biomedical Applications, Nanxi Wang 2017 University of Nebraska-Lincoln

Genetic Code Expansion In Biochemical Investigations And Biomedical Applications, Nanxi Wang

Student Research Projects, Dissertations, and Theses - Chemistry Department

Genetic code expansion provides a powerful tool for site-specific incorporation of unnatural amino acids (unAAs) with novel biochemical and physiological properties into proteins in live cells and organisms. To achieve this, a nonsense codon suppression system, which consists of an orthogonal aminoacyl-tRNA synthetase (aaRS) and tRNA pair that specifically decodes a nonsense codon (e.g., amber codon and quadruplet codon) with an unAA but do not “cross talk” with their endogenous counterparts, was established. This Ph.D. thesis presents our efforts on evolution and application of nonsense codon suppression systems for biochemical and biomedical investigations.

In Chapter 1, a brief ...


Design And Optimization Of Rsv F For Vaccination, Elizabeth R. Wilson 2017 Iowa State University

Design And Optimization Of Rsv F For Vaccination, Elizabeth R. Wilson

Honors Projects and Posters

Respiratory syncytial virus (RSV) is a common infection that affects most infants by the age of two, as well as a large percentage of the elderly population. Currently, the majority of prevention lies in the hands of the caretakers: wash hands frequently, avoid contact if sick or have babies wear masks, keep young children away from babies, avoid crowds during outbreaks. As these methods are not always feasible, this project aims to develop a vaccine for RSV by focusing on the fusion (F) glycoprotein. This protein is necessary for the virus to infect, as it assists the virus in fusing ...


Influence Of Lysine-Based Biomaterials On Fibroblast To Myofibroblast Differentiation, Catherine Le Denmat 2017 Iowa State University

Influence Of Lysine-Based Biomaterials On Fibroblast To Myofibroblast Differentiation, Catherine Le Denmat

Honors Projects and Posters

Fibrous encapsulation occurs as a result of implantation of devices such as pacemakers, artificial breast implants, and microencapsulated islet cells used in type 1 diabetes treatment. The foreign body response (FBR) is responsible for the development of a fibrous capsule, which is often detrimental to the function of the implanted device and therefore affected patients. One event leading to fibrous capsule formation is contraction of collagen by myofibroblasts. The objective of this project was to significantly reduce the thickness of the fibrous capsule by limiting fibroblast to myofibroblast differentiation. It was hypothesized that using lysine-based biomaterials with amidine-like functional group ...


An Expedited, Regiospecific Para-Bromination Of Activated Aryls, Kathryn E. Dudley 2017 Western Kentucky University

An Expedited, Regiospecific Para-Bromination Of Activated Aryls, Kathryn E. Dudley

Masters Theses & Specialist Projects

Electrophilic Aromatic Substitution (EAS) is one of the most frequently used aryl substitution methods. Aside from the fact that most EAS reactions require an acid and an oxidizer to proceed, the reactions involving activated aryls typically produce a mixture of ortho- and para- products as well as an ortho-/para- disubstituted product. Regiospecificity in aromatic substitution is key in the production of many compounds in a variety of disciplines. Since EAS is one of the most often used substitution methods, it is extremely important to develop an efficient method for regiospecific substitutions. Previous research developed a method of ortho-substitution by ...


Removal Of Chlorine From Chlorine-Nitrogen Mixture In A Film Of Liquid Water, Sarwan S. Sandhu 2017 University of Dayton

Removal Of Chlorine From Chlorine-Nitrogen Mixture In A Film Of Liquid Water, Sarwan S. Sandhu

Sarwan S. Sandhu

In industry there are many examples of absorption of a gas with or without chemical reaction in the liquid phase. In physical absorption, a particular gaseous component is removed from a gas mixture due to its larger solubility in the liquid phase solvent. The removal of butane and pentane from a refinery gas mixture by a heavy oil in the liquid phase is an example of physical absorption. In absorption with chemical reaction, the gaseous component to be removed transfers across the gas-liquid interface due to a difference in the bulk chemical potentials or concentrations in the two phases. The ...


Disinfection By-Products Formation After Coagulation Of Algal Extracellular And Intracellular Organic Matters, Tianyiru Chen 2017 The University of Western Ontario

Disinfection By-Products Formation After Coagulation Of Algal Extracellular And Intracellular Organic Matters, Tianyiru Chen

Electronic Thesis and Dissertation Repository

Algal organic matter (AOM) is found in high concentration during algal bloom season in drinking water systems, which is generally categorized into extracellular organic matter (EOM) and intracellular organic matter (IOM). These compounds are not well removed in traditional water treatment methods such as coagulation, and are the precursors of subsequent disinfection by-products (DBPs) during chlorination of water. In this study, EOM and IOM content of four different algae were quantified measuring dissolved organic carbon (DOC), UV absorbance at 254 nm and turbidity. Coagulation using alum (Al2(SO4)3•16H2O)was used to remove the algal ...


High-Density Non-Fouling Bioactive Coatings: Development And Characterization, Bonan Yu, Ramya Raman, Karl Schlike 2017 Oregon State University

High-Density Non-Fouling Bioactive Coatings: Development And Characterization, Bonan Yu, Ramya Raman, Karl Schlike

Biomedical Engineering Western Regional Conference

No abstract provided.


Molecular Modeling Of Antibody-Antigen Binding Near Solid Surfaces, Derek Bush, Thomas Knotts 2017 Brigham Young University

Molecular Modeling Of Antibody-Antigen Binding Near Solid Surfaces, Derek Bush, Thomas Knotts

Biomedical Engineering Western Regional Conference

Antibody microarrays are biosensors that have the potential to revolutionize molecular detection in medicine, scientific research, and national defense. However, current microarrays are not widely used due to problems including poor reproducibility and signal quality, unbalanced antibody performance, and cross-reactivity. Prior work in the area focused mainly on the stability of the antibody alone and not its affinity for its antigen. This presentation shows results of using molecular simulation to determine how different types of surfaces affect antigen binding to surface-tethered antibodies. The results offer an unprecedented, molecular-level view into these protein-protein-surface interactions and how to drive binding to occur.


Candida Albicans Stimulates Streptococcus Mutans Microcolony Development Via Cross-Kingdom Biofilm-Derived Metabolites, Dongyeop Kim, Arjun Sengupta, Tagbo H. R Niepa, Byung-Hoo Lee, Aalim Weljie, Veronica S. Freitas-Blanco, Ramiro M. Murata, Kathleen J. Stebe, Daeyeon Lee, Hyun Koo 2017 University of Pennsylvania

Candida Albicans Stimulates Streptococcus Mutans Microcolony Development Via Cross-Kingdom Biofilm-Derived Metabolites, Dongyeop Kim, Arjun Sengupta, Tagbo H. R Niepa, Byung-Hoo Lee, Aalim Weljie, Veronica S. Freitas-Blanco, Ramiro M. Murata, Kathleen J. Stebe, Daeyeon Lee, Hyun Koo

Departmental Papers (CBE)

Candida albicans is frequently detected with heavy infection of Streptococcus mutans in plaque-biofilms from children affected with early-childhood caries, a prevalent and costly oral disease. The presence of C. albicans enhances S. mutans growth within biofilms, yet the chemical interactions associated with bacterial accumulation remain unclear. Thus, this study was conducted to investigate how microbial products from this cross-kingdom association modulate S. mutans build-up in biofilms. Our data revealed that bacterial-fungal derived conditioned medium (BF-CM) significantly increased the growth of S. mutans and altered biofilm 3D-architecture in a dose-dependent manner, resulting in enlarged and densely packed bacterial cell-clusters (microcolonies). Intriguingly ...


Engineering A Fluorescent Protease Sensor For In Vivo Protein Detection, Thomas C. Kinard 2017 University of Connecticut

Engineering A Fluorescent Protease Sensor For In Vivo Protein Detection, Thomas C. Kinard

Honors Scholar Theses

This report details the results of an ongoing project to engineer a mutant form of Red Fluorescent Protein (RFP) variant mCherry that acts as a real-time in vivo protease sensor. The sought-after mutant only becomes fluorescent when exposed to Tobacco Etch Virus (TEV) Protease, this system’s model protease. This will be accomplished via the insertion of the TEV Protease Recognition Site (TEV-PRS) in such a position that, before cleavage, will prevent the protein from folding to fluorescent conformation, but upon cleavage, will allow for fluorescent conformation to occur. The cylindrical structure of the protein, composed of beta-pleated sheets, contains ...


Photoelectrochemical Cell Constructed From Bby Membrane With Various Substrate Materials, Yang Liu 2017 Virginia Community Colleges

Photoelectrochemical Cell Constructed From Bby Membrane With Various Substrate Materials, Yang Liu

Theses and Dissertations

Photoelectrochemical cells have been intensively studied in recent years with regard to using thylakoid and photosynthesis system I/II. BBY membrane is another protein complex that has potential to be utilized to build photoelectrochemical cells. Within the BBY membrane lies the highly active photosynthesis system II complex, which upon light activation, generates electrons transported within the electron transport chain during photosynthesis in green plants. This study presents an approach of immobilizing thylakoid or BBY membrane onto gold nanoparticle modified gold plate or multi-walled carbon nanotube (MWCNT) modified indium tin oxide vi (ITO) coated glass substrate. The results show that BBY ...


An Investigation Of Lsf-Ysz Conductive Scaffolds For Infiltrated Sofc Cathodes, Yuan Cheng, Tae-Sik Oh, Rachel Wilson, Raymond J. Gorte, John M. Vohs 2017 University of Pennsylvania

An Investigation Of Lsf-Ysz Conductive Scaffolds For Infiltrated Sofc Cathodes, Yuan Cheng, Tae-Sik Oh, Rachel Wilson, Raymond J. Gorte, John M. Vohs

Departmental Papers (CBE)

Porous compostites of Sr-doped LaFeO3 (LSF and yttria-stabilized zirconia (YSZ) were investigated as conductive scaffolds for infiltrated SOFC cathodes with the goal of producing scaffolds for which only a few perovskite infiltration steps are required to achieve sufficient conductivity. While no new phases form when LSF-YSZ composites are calcined to 1623 K, shifts in the lattice parameters indicate Zr can enter the perovskite phase. Measurements on dense, LSF-YSZ composites show that the level of Zr doping depends on the Sr:La ration. Because conductivity of undoped LSF increases with Sr content while both the iconic and electronic conductivities of ...


Modification Of Lsf-Ysz Composite Cathodes By Atomic Layer Deposition, M. Rahmanipour, Yuan Cheng, Tzia M. Onn, A. Donazzi, John M. Vohs, Raymond J. Gorte 2017 University of Pennsylvania

Modification Of Lsf-Ysz Composite Cathodes By Atomic Layer Deposition, M. Rahmanipour, Yuan Cheng, Tzia M. Onn, A. Donazzi, John M. Vohs, Raymond J. Gorte

Departmental Papers (CBE)

composite, Solid-Oxide-Fuel-Cell (SOFC) electrodes of La0.8Sr0.2FeO3 (LSF) and yttria-stabilized zirconia (YSZ) were prepared by infiltration methods and then modified by Atomic Layer Deposition (ALD) of ZrO2, La2O3, Fe2O3, or La2O3-Fe2O3 codeposited films of different thicknesses to determine the effect of surface composition on cathode performance. Film growth rates for ALD performed using vacuum procedures at 573 K for Fe2O3 and 523 K for ZrO2 and La2O3 were determined to be 0.024 ...


Surface Enabled Lab-On-A-Chip (Loc) Device For Protein Detection And Separation, Zhichao Wang 2017 Michigan Technological University

Surface Enabled Lab-On-A-Chip (Loc) Device For Protein Detection And Separation, Zhichao Wang

Dissertations, Master's Theses and Master's Reports

Sensitive and selective chemical/biological detection/analysis for proteins is essential for applications such as disease diagnosis, species phenotype identification, product quality control, and sample examination. Lab-on-a-chip (LOC) device provides advantages of fast analysis, reduced amount of sample requirements, and low cost, to magnificently facilitate protein detection research. Isoelectric focusing (IEF) is a strong and reliable electrophoretic technique capable of discerning proteins from complex mixtures based on the isoelectric point (pI) differences. It has experienced plenty of fruitful developments during previous decades which has given it the capability of performing with highly robust and reproducible analysis. This progress has made ...


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