High-Density Non-Fouling Bioactive Coatings: Development And Characterization, 2017 Oregon State University
High-Density Non-Fouling Bioactive Coatings: Development And Characterization, Bonan Yu, Ramya Raman, Karl Schlike
Biomedical Engineering Western Regional Conference
No abstract provided.
Molecular Modeling Of Antibody-Antigen Binding Near Solid Surfaces, 2017 Brigham Young University
Molecular Modeling Of Antibody-Antigen Binding Near Solid Surfaces, Derek Bush, Thomas Knotts
Biomedical Engineering Western Regional Conference
Antibody microarrays are biosensors that have the potential to revolutionize molecular detection in medicine, scientific research, and national defense. However, current microarrays are not widely used due to problems including poor reproducibility and signal quality, unbalanced antibody performance, and cross-reactivity. Prior work in the area focused mainly on the stability of the antibody alone and not its affinity for its antigen. This presentation shows results of using molecular simulation to determine how different types of surfaces affect antigen binding to surface-tethered antibodies. The results offer an unprecedented, molecular-level view into these protein-protein-surface interactions and how to drive binding to occur.
Candida Albicans Stimulates Streptococcus Mutans Microcolony Development Via Cross-Kingdom Biofilm-Derived Metabolites, 2017 University of Pennsylvania
Candida Albicans Stimulates Streptococcus Mutans Microcolony Development Via Cross-Kingdom Biofilm-Derived Metabolites, Dongyeop Kim, Arjun Sengupta, Tagbo H. R Niepa, Byung-Hoo Lee, Aalim Weljie, Veronica S. Freitas-Blanco, Ramiro M. Murata, Kathleen J. Stebe, Daeyeon Lee, Hyun Koo
Departmental Papers (CBE)
Candida albicans is frequently detected with heavy infection of Streptococcus mutans in plaque-biofilms from children affected with early-childhood caries, a prevalent and costly oral disease. The presence of C. albicans enhances S. mutans growth within biofilms, yet the chemical interactions associated with bacterial accumulation remain unclear. Thus, this study was conducted to investigate how microbial products from this cross-kingdom association modulate S. mutans build-up in biofilms. Our data revealed that bacterial-fungal derived conditioned medium (BF-CM) significantly increased the growth of S. mutans and altered biofilm 3D-architecture in a dose-dependent manner, resulting in enlarged and densely packed bacterial cell-clusters (microcolonies). Intriguingly ...
Engineering A Fluorescent Protease Sensor For In Vivo Protein Detection, 2017 University of Connecticut
Engineering A Fluorescent Protease Sensor For In Vivo Protein Detection, Thomas C. Kinard
Honors Scholar Theses
This report details the results of an ongoing project to engineer a mutant form of Red Fluorescent Protein (RFP) variant mCherry that acts as a real-time in vivo protease sensor. The sought-after mutant only becomes fluorescent when exposed to Tobacco Etch Virus (TEV) Protease, this system’s model protease. This will be accomplished via the insertion of the TEV Protease Recognition Site (TEV-PRS) in such a position that, before cleavage, will prevent the protein from folding to fluorescent conformation, but upon cleavage, will allow for fluorescent conformation to occur. The cylindrical structure of the protein, composed of beta-pleated sheets, contains ...
An Investigation Of Lsf-Ysz Conductive Scaffolds For Infiltrated Sofc Cathodes, 2017 University of Pennsylvania
An Investigation Of Lsf-Ysz Conductive Scaffolds For Infiltrated Sofc Cathodes, Yuan Cheng, Tae-Sik Oh, Rachel Wilson, Raymond J. Gorte, John M. Vohs
Departmental Papers (CBE)
Porous compostites of Sr-doped LaFeO3 (LSF and yttria-stabilized zirconia (YSZ) were investigated as conductive scaffolds for infiltrated SOFC cathodes with the goal of producing scaffolds for which only a few perovskite infiltration steps are required to achieve sufficient conductivity. While no new phases form when LSF-YSZ composites are calcined to 1623 K, shifts in the lattice parameters indicate Zr can enter the perovskite phase. Measurements on dense, LSF-YSZ composites show that the level of Zr doping depends on the Sr:La ration. Because conductivity of undoped LSF increases with Sr content while both the iconic and electronic conductivities of ...
Modification Of Lsf-Ysz Composite Cathodes By Atomic Layer Deposition, 2017 University of Pennsylvania
Modification Of Lsf-Ysz Composite Cathodes By Atomic Layer Deposition, M. Rahmanipour, Yuan Cheng, Tzia M. Onn, A. Donazzi, John M. Vohs, Raymond J. Gorte
Departmental Papers (CBE)
composite, Solid-Oxide-Fuel-Cell (SOFC) electrodes of La0.8Sr0.2FeO3 (LSF) and yttria-stabilized zirconia (YSZ) were prepared by infiltration methods and then modified by Atomic Layer Deposition (ALD) of ZrO2, La2O3, Fe2O3, or La2O3-Fe2O3 codeposited films of different thicknesses to determine the effect of surface composition on cathode performance. Film growth rates for ALD performed using vacuum procedures at 573 K for Fe2O3 and 523 K for ZrO2 and La2O3 were determined to be 0.024 ...
Understanding Glycoside Hydrolase Processivity For Improved Biomass Conversion, 2017 University of Kentucky
Understanding Glycoside Hydrolase Processivity For Improved Biomass Conversion, Suvamay Jana
Theses and Dissertations--Chemical and Materials Engineering
In nature, organisms secrete synergistic enzyme cocktails to deconstruct crystalline polysaccharides, such as cellulose and chitin, to soluble sugars. The cocktails consist of multiple classes of processive and non-processive glycoside hydrolases (GH) that aid in substrate accessibility and reduce product inhibition. Processive GHs attach to chain ends and hydrolyze many glycosidic linkages in sequence to produce disaccharide units before dissociation, and as such, are responsible for the majority of hydrolytic bond cleavages. Accordingly, processive GHs are targets for activity improvements towards efficient and economical biomass conversion. However, the mechanism and factors responsible for processivity are still not understood completely at ...
Biomimetic Devices To Drive A Thermodynamically Uphill Reaction Using Light And To Degrade Industrial Waste Stream Components, Madison Joanne Sloan
Theses and Dissertations--Chemistry
Given the amount of industrial waste produced each year, as well as the accruing amount of greenhouse gases in our atmosphere produced by the burning of fossil fuels, it is imperative that humanity develop environmentally-sustainable sources of energy and methods of remediation. Nature achieves both of these by use of enzymes as catalysts, inspiring interest in designing biomimetic systems capable of harnessing clean energy and remediating industrial waste. This study examined the ability of enzymes in electrochemical and convective flow systems to achieve these tasks. The first portion studied the incorporation of enzymes into an electrochemical system to drive the ...
Vitreous Gel Physics, 2017 University of Massachusetts - Amherst
Vitreous Gel Physics, Svetlana Morozova
The transparent vitreous, which fills the posterior cavity of the eye, is incredibly engineered. The charged polyelectrolyte hyaluronic acid (HA) network swells to maintain the pressure in the eye, while stiff collagen type II bundles help absorb any external mechanical shock. Our investigations have contributed to a few key developments related to the physical properties of the vitreous:
(1) The stiff collagen network that supports the soft gel network is self-assembled from single triple-helix collagen proteins. Electrostatic interactions drive this assembly, such that the size and concentration are optimized at physiological salt concentrations. The width of the assemblies remarkably changes ...
Enzymatically Active Microspheres For Self-Propelled Colloidal Engines, 2017 CUNY City College of New York
Enzymatically Active Microspheres For Self-Propelled Colloidal Engines, Jungeun Park
Micro- and nano-motors have attracted numerous attentions from various scientific areas due to their potential applications. Most studies on self-propelled colloidal engines have exploited catalytic decomposition of hydrogen peroxide to drive the motor. Since the hydrogen peroxide is caustic, it is not suitable to use in biological applications, encouraging people to develop “greener” fuels. The aim of this research is to study a new transduction mechanism for self-propulsion not tied to hydrogen peroxide, and which can in particular be used with biological molecules as fuels. In this study, we focus on making particles with enzymatic activity which can effectively decompose ...
Novel Approaches To Bioengineering Target Identification: A Focus On Non-Genetic Contributions To Complex Bacterial Phenotypes, 2017 University of Colorado at Boulder
Novel Approaches To Bioengineering Target Identification: A Focus On Non-Genetic Contributions To Complex Bacterial Phenotypes, Keesha Elizabeth Erickson
Chemical & Biological Engineering Graduate Theses & Dissertations
Many critical societal issues could be alleviated via advances in biological engineering, specifically those bolstering our ability to design complex phenotypes like bacterial tolerance, resistance, and chemical production. Complex phenotypes are controlled through overlapping regulatory mechanisms encompassing multiple systems, including metabolism, cellular structure, energy consumption, and more. Moreover, immense heterogeneity within and across bacterial populations makes it challenging to discern key genes and pathways contributing to phenotypes of interest. Thus, to enable engineering of complex bacterial phenotypes, it is essential to consider unconventional perspectives from which to identify targets.
Here, we describe efforts concentrated on non-genetic data to ascertain key ...
Surface Enabled Lab-On-A-Chip (Loc) Device For Protein Detection And Separation, 2017 Michigan Technological University
Surface Enabled Lab-On-A-Chip (Loc) Device For Protein Detection And Separation, Zhichao Wang
Dissertations, Master's Theses and Master's Reports
Sensitive and selective chemical/biological detection/analysis for proteins is essential for applications such as disease diagnosis, species phenotype identification, product quality control, and sample examination. Lab-on-a-chip (LOC) device provides advantages of fast analysis, reduced amount of sample requirements, and low cost, to magnificently facilitate protein detection research. Isoelectric focusing (IEF) is a strong and reliable electrophoretic technique capable of discerning proteins from complex mixtures based on the isoelectric point (pI) differences. It has experienced plenty of fruitful developments during previous decades which has given it the capability of performing with highly robust and reproducible analysis. This progress has made ...
Cis,Cis-Muconic Acid Isomerization And Catalytic Conversion To Biobased Cyclic-C6-1,4-Diacid Monomers, 2017 Iowa State University
Cis,Cis-Muconic Acid Isomerization And Catalytic Conversion To Biobased Cyclic-C6-1,4-Diacid Monomers, Jack M. Carraher, Toni Pfennig, Radhika Rao, Brent H. Shanks, Jean-Philippe Tessonnier
Chemical and Biological Engineering Publications
Renewable terephthalic and 1,4-cyclohexanedicarboxylic acids can be produced from biomass via muconic acid using a combination of biological and chemical processes. In this conversion scheme, cis,cis-mucononic acid is first obtained by fermentation using either sugar or lignin monomers as a feedstock. The diunsaturated cis,cis-diacid is then isomerized to trans,trans-muconic acid, reacted with biobased ethylene through Diels–Alder cycloaddition, and further hydrogenated or dehydrogenated to yield the desired 100% renewable cyclic dicarboxylic acid. The isomerization of cis,cis- to trans,trans-muconic acid represents the main bottleneck in this process due to undesired side reactions that promote ring ...
Metabolic Modeling And Engineering Of Gas Fermentation In Bubble Column Reactors, 2017 University of Massachusetts Amherst
Metabolic Modeling And Engineering Of Gas Fermentation In Bubble Column Reactors, Jin Chen
Gas fermentation is an attractive route to produce alternative fuels and chemicals from non-food feedstocks, such as waste gas streams from steel mills and synthesis gas (mainly CO and H2) produced from municipal solid waste through gasification. While commercial development of gas fermentation technology is underway, many research problems must be addressed to further advance the technology towards economic competitiveness. A particularly important challenge is to develop integrated metabolic and transport models that describe gas fermentation in industrially relevant bubble column reactors.
I have developed and evaluated a spatiotemporal metabolic model for bubble column reactors with the syngas fermenting ...
The Influence Of Light On The Electrochemical Characteristics Of Pure Aluminum, 2017 The University of Akron
The Influence Of Light On The Electrochemical Characteristics Of Pure Aluminum, Hongbo M. Cong
Honors Research Projects
The objective of this work was to study the influence of light (wave length ~ 460 nm) on the electrochemical characteristics of pure aluminum. The open circuit potential (OCP) measurements, potentiostatic polarization, cyclic potentiodynamic polarization and Zero Resistance Ammetry (ZRA) were performed in 0.1 M NaCl solution. It was observed that the incident light shifted the OCP in the noble direction by 100 mV. Furthermore, ZRA was performed to measure the galvanic current between illuminated and non-illuminated aluminum electrodes. Cyclic potentiodynamic polarization tests revealed the influence of light on the pitting potential, passive current density, corrosion current density, and repassivation ...
The Effects Of Adaptogens On The Physical And Psychological Symptoms Of Chronic Stress, 2017 Georgia State University
The Effects Of Adaptogens On The Physical And Psychological Symptoms Of Chronic Stress, Tosin O. Ajala
DISCOVERY: Georgia State Honors College Undergraduate Research Journal
Stress is a state of disharmony or threatened homeostasis. The maintenance of homeostasis in stages of internal or external challenges, called stressors, requires constant adjustments of hormonal, behavioral, and autonomic functions. The nuances of life may bring about unwanted stress to the human body. Some of the top causes of stress in America include work, finances, relationships, and health. There are numerous physical and psychological symptoms associated with long-term “chronic” stress, which include chronic fatigue, frustration, irritability, insomnia, frequent headaches, chest and back pain, weakness, and weight gain or weight loss. These symptoms of chronic stress are associated with depression ...
Designing And Development Of A Photobioreactor For Optimizing The Growth Of Micro Algae And Studying Its Growth Parameters, 2017 South Dakota State University
Designing And Development Of A Photobioreactor For Optimizing The Growth Of Micro Algae And Studying Its Growth Parameters, Sarmila Katuwal
Electronic Theses and Dissertations
This thesis presents the estimated value of materials required to grow 1g of biomass and the analysis of the light intensity with respect to flow path and flow rate. This thesis aims to design the sparger for a flat plate Photobioreactor, study the flow patterns at different flow rate of air flow and check the performance of flat plate PBR by growing the cyanobacteria. The estimated value to produce 1g of biomass (C44.6H7O25N7.68P0.9S0.3) was 0.099g of N, 0.493g of C, 0.160 g ...
Photoelectrochemical Cell Constructed From Bby Membrane With Various Substrate Materials, 2017 Virginia Community Colleges
Photoelectrochemical Cell Constructed From Bby Membrane With Various Substrate Materials, Yang Liu
Theses and Dissertations
Photoelectrochemical cells have been intensively studied in recent years with regard to using thylakoid and photosynthesis system I/II. BBY membrane is another protein complex that has potential to be utilized to build photoelectrochemical cells. Within the BBY membrane lies the highly active photosynthesis system II complex, which upon light activation, generates electrons transported within the electron transport chain during photosynthesis in green plants. This study presents an approach of immobilizing thylakoid or BBY membrane onto gold nanoparticle modified gold plate or multi-walled carbon nanotube (MWCNT) modified indium tin oxide vi (ITO) coated glass substrate. The results show that BBY ...
Protein Release Study Using Heparin Modified Methacrylamide Chitosan Hydrogels, 2017 The University of Akron
Protein Release Study Using Heparin Modified Methacrylamide Chitosan Hydrogels, Dakotah G. Cox
Honors Research Projects
This study was performed to improve the release of proteins from hydrogels in order to increase their effectiveness in treating spinal cord injuries. Heparin, which has the ability to bind to proteins and slow their release, underwent methacrylation, was added to prepared methacrylamide chitosan (MAC) solutions with a model protein (SDF-1α) and photopolymerized to create MAC-heparin hydrogels. Varying weight percentages of methacrylated heparin were tested in order to determine the optimal amount needed to improve the release profile. The pure MAC hydrogels and gels with 10 wt% heparin had a rapid release of the SDF-1α (>23% and >29%, respectively, in ...
Theoretical And Experimental Investigation Of Water-Gas Shift Reaction Over Supported Copper/Iron Oxide Catalysts, Ozgen Yalcin
Theses and Dissertations
Industrial hydrogen production from various resources is driven by the worldâs advancement towards a hydrogen economy. The water-gas shift (WGS) catalytic reaction is an intermediate step between reforming of carbonaceous materials and hydrogen production. This catalytic reaction is important for hydrogen enrichment and carbon monoxide reduction. Copper-supported chromium-iron oxide (Fe3O4-Cr2O3-CuO) is the main catalyst system for the high temperature (HT) WGS redox reaction.Density functional theory calculations were carried out to investigate the role of the chromium and copper, which were demonstrated to act as textural and catalytic promoters, respectively, for the Fe3O4-Cr2O3-CuO catalyst system by in situ experimental ...