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Overcoming Technological Challenges For The Commercialization Of The Circulating Fluidized Bed Bioreactor For Municipal Wastewater Treatment, Michael J. Nelson 2021 The University of Western Ontario

Overcoming Technological Challenges For The Commercialization Of The Circulating Fluidized Bed Bioreactor For Municipal Wastewater Treatment, Michael J. Nelson

Electronic Thesis and Dissertation Repository

The fluidized bed bioreactor as an attached growth wastewater treatment process has demonstrated advantages over suspended growth processes for municipal wastewater treatment applications. However, previous studies have also demonstrated potentially serious disadvantages in terms of energy consumption and maximum reactor size of high flow applications.

In this work, a cost analysis using the CapdetWorks, supplemented by calibrated model data taken from GPS-X was performed to determine the cost effectiveness of the circulating fluidized bed bioreactor (CFBBR). This study demonstrated that the CFBBR is most cost competitive at low flow below 5 MGD. A 10%-20% reduction in net present values ...


Towards Understanding Protein Immobilization Rules Through Site-Specific Covalent Immobilization Of T4 Lysozyme, Maxwell Hilbert 2021 Clemson University

Towards Understanding Protein Immobilization Rules Through Site-Specific Covalent Immobilization Of T4 Lysozyme, Maxwell Hilbert

All Dissertations

Soluble enzymes have the unique ability to assist the conversion of complex substrates in fewer steps compared to conventional chemical synthesis; however, when immobilized to a surface, the insoluble enzyme's activity and stability can suffer in its new environment. To improve upon future immobilized enzyme efficacy, a fundamental understanding of the interactions across the enzyme, linker, and surface during covalent, site-specific immobilization that can be detrimental to its activity is required. More specifically, using a well-characterized and model enzyme, T4 lysozyme (T4L), 28 different cysteine attachment points' effects on site-specifically immobilized activity are examined. Different combinations of heterobifunctional amine-to-sulfhydryl ...


Antibody-Drug Nanoparticle Induces Synergistic Treatment Efficacies In Her2 Positive Breast Cancer Cells, Muhammad Raisul Abedin, Kaitlyne Powers, Rachel Aiardo, Dibbya Barua, Sutapa Barua 2021 Missouri University of Science and Technology

Antibody-Drug Nanoparticle Induces Synergistic Treatment Efficacies In Her2 Positive Breast Cancer Cells, Muhammad Raisul Abedin, Kaitlyne Powers, Rachel Aiardo, Dibbya Barua, Sutapa Barua

Chemical and Biochemical Engineering Faculty Research & Creative Works

Chemotherapeutic drugs suffer from non-specific binding, undesired toxicity, and poor blood circulation which contribute to poor therapeutic efficacy. In this study, antibody–drug nanoparticles (ADNs) are engineered by synthesizing pure anti-cancer drug nanorods (NRs) in the core of nanoparticles with a therapeutic monoclonal antibody, Trastuzumab on the surface of NRs for specific targeting and synergistic treatments of human epidermal growth factor receptor 2 (HER2) positive breast cancer cells. ADNs were designed by first synthesizing ~ 95 nm diameter × ~ 500 nm long paclitaxel (PTX) NRs using the nanoprecipitation method. The surface of PTXNRs was functionalized at 2′ OH nucleophilic site using carbonyldiimidazole ...


Continuous Liquid-Liquid Extraction Of Lomustine Synthesis, Devna Grover 2021 Purdue University

Continuous Liquid-Liquid Extraction Of Lomustine Synthesis, Devna Grover

The Journal of Purdue Undergraduate Research

No abstract provided.


Metabolic Modeling Of Cystic Fibrosis Airway Microbiota From Patient Samples, Arsh Vyas 2021 University of Massachusetts Amherst

Metabolic Modeling Of Cystic Fibrosis Airway Microbiota From Patient Samples, Arsh Vyas

Masters Theses

Cystic Fibrosis (CF) is a genetic disorder, found with higher prevalence in the Caucasian population, affecting > 30,000 individuals in the United States and > 70,000 worldwide. Due to the astoundingly high rate of mortality among CF patients being attributed to respiratory failure brought on by chronic bacterial infections and subsequent airway inflammation, there has been a lot of focus on systematically analyzing CF lung airway communities. While it is observed traditionally that Pseudomonas aeruginosa is the most threatening and persistent CF colonizer due to high antibiotic resistance, recent studies have elicited the roles of other pathogens and it has ...


Spatiotemporal Metabolic Modeling Of Pseudomonas Aeruginosa Biofilm Expansion, Robert Sourk 2021 University of Massachusetts at Amherst

Spatiotemporal Metabolic Modeling Of Pseudomonas Aeruginosa Biofilm Expansion, Robert Sourk

Masters Theses

Spatiotemporal metabolic modeling of microbial metabolism is a step closer to achieving higher dimensionalities in numerical studies (in silico) of biofilm maturation. Dynamic Flux Balance Analysis (DFBA) is an advanced modeling technique because this method incorporates Genome Scale Metabolic Modeling (GSMM) to compute the biomass growth rate and metabolite fluxes. Biofilm thickness is pertinent because this variable of biofilm maturation can be measured in a laboratory (in vitro). Pseudomonas aeruginosa (P. aeruginosa) is the model bacterium used in this computational model based on previous research conducted by Dr. Michael Henson, available GSMMs, and the societal significance of patients suffering from ...


Metabolic Modeling Of Bacterial Co-Cultures For Co-To-Butyrate Conversion In Bubble Column Bioreactors, Naresh Kandlapalli 2021 University of Massachusetts Amherst

Metabolic Modeling Of Bacterial Co-Cultures For Co-To-Butyrate Conversion In Bubble Column Bioreactors, Naresh Kandlapalli

Masters Theses

One of the most promising routes to renewable liquid fuels and chemicals is the fermentation of waste carbon by specialized microbes. Commercial development of gas fermentation technology is underway but many fundamental research problems must be addressed to further advance the technology towards economic competitiveness. This thesis addresses the important problem of developing integrated metabolic and transport models that predict gas fermentation performance in industrially relevant bubble column reactors. The computational models describe the conversion of CO-rich waste streams including synthesis gas to the platform chemical butyrate. The proposed modeling approach involves combining genome-scale reconstructions of bacterial species metabolism with ...


Open-Ended Hollow Coaxial Cable Resonator Sensor, Jie Huang, Chen Zhu, Rex E. Gerald II 2021 Missouri University of Science and Technology

Open-Ended Hollow Coaxial Cable Resonator Sensor, Jie Huang, Chen Zhu, Rex E. Gerald Ii

Electrical and Computer Engineering Faculty Research & Creative Works

An open-ended hollow coaxial cable resonator probe configured to receive an aerosol sample for analysis. A metal post shorts the resonator's inner and outer conductors. A metal plate is spaced apart from an open end of the resonator by a dielectric layer that contains the received aerosol sample. Interrogator circuitry coupled to the resonator transmits an electromagnetic wave within the resonator and generates an electric field at the open end of the resonator. The interrogator circuitry is responsive to the generated electric field for determining a resonance frequency and an impedance of the resonator when the aerosol sample is ...


Study Level Recovery Processes Of Thorium And Several Rare Earth Elements From Monazite Ore, Makayla Hyde, Jaclyn Choate 2021 University of Tennessee, Knoxville

Study Level Recovery Processes Of Thorium And Several Rare Earth Elements From Monazite Ore, Makayla Hyde, Jaclyn Choate

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Monazite ore is composed of several minerals including several rare earth element (REE) and thorium phosphates. Many past research projects have looked at the extraction of REE from monazite ore but have discarded the thorium phosphate compound as waste. Since thorium could be a possible fuel for state-of-the-art future nuclear reactors, this research focuses on a way to recover thorium from the monazite ore. The overall purpose of this research is to develop a conceptual recovery process that recovers thorium dioxide from monazite ore. In some parts of the world where uranium sources are limited; then, thorium could be used ...


Co2 Derived Carbon Capture Using Microalgae In A Photobiocrec Unit, Maureen D. Cordoba Perez 2021 The University of Western Ontario

Co2 Derived Carbon Capture Using Microalgae In A Photobiocrec Unit, Maureen D. Cordoba Perez

Electronic Thesis and Dissertation Repository

Microalgae has the potential to contribute to carbon dioxide capture, resulting in the production of alternative fuels and valuable chemical products. To accomplish this, high-efficiency photobioreactors must be conceptualized, designed, and established, in order to achieve high inorganic carbon conversion, superior light utilization, and unique fluid dynamics.

In this PhD Dissertation, experiments with Chlorella vulgaris were carried out, in a 0.175L especially designed PhotoBioCREC unit, under controlled radiation and high mixing conditions. This unique design involves 1 mm-2 mm alumina particles, which keep photoreactor walls always clean, without compromising photon transmittance. Sodium bicarbonate (NaHCO3) was supplied as the ...


Biocompatible In Situ Gelling Hydrogel Formulations For Intra-Articular Drug Delivery, Charlotte E. Thomsen 2021 Western University

Biocompatible In Situ Gelling Hydrogel Formulations For Intra-Articular Drug Delivery, Charlotte E. Thomsen

Undergraduate Student Research Internships Conference

No abstract provided.


Methane Emission Treatment And Potential Upcycling Using Methylomicrobium Alcaliphilum, Jace Parkinson 2021 Utah State University

Methane Emission Treatment And Potential Upcycling Using Methylomicrobium Alcaliphilum, Jace Parkinson

All Graduate Plan B and other Reports

Anthropogenic methane emissions are harmful to the environment and can be difficult to treat. Use of M. alcaliphilum for bioremediation of methane looks to be one of the most promising methods of reducing the negative environmental impacts of methane emissions while simultaneously providing an economic incentive. Successful bioreactor design for M. alcaliphilum based methane treatment requires biokinetic constants and microbial growth information. Methods were established for determining biokinetic constants, including growth rate, biomass yield, specific methane consumption rate, and a correlation between methane oxidation and carbon dioxide production for M. alcaliphilum. Values determined for these constants were used to provide ...


Finite Element Modelling And Exergy Analysis Of A Solar Thermal Hollow Fiber Vacuum Membrane Distillation System, Benjamin Shuldes 2021 University of Nebraska-Lincoln

Finite Element Modelling And Exergy Analysis Of A Solar Thermal Hollow Fiber Vacuum Membrane Distillation System, Benjamin Shuldes

Chemical & Biomolecular Engineering Theses, Dissertations, & Student Research

A finite element model was developed to investigate the performance of a vacuum membrane distillation module under various operating conditions and membrane parameters. Porosity, tortuosity, pore diameter, membrane thickness, and fiber length were varied along with feed temperature, velocity, and flow configuration. In all cases, boundary layer polarization phenomena were seen to inhibit the performance of the module. At certain conditions, for a 7.5 cm fiber, the reduction in permeate flux from 65 LMH (Liter/m2/h) at the inlet to below 45 LMH at the outlet of the fibers was observed. In most cases, salt concentration polarization ...


Metabolic Modeling And Omics-Integrative Analysis Of Single And Multi-Organism Systems: Discovery And Redesign, Mohammad Mazharul Islam 2021 University of Nebraska-Lincoln

Metabolic Modeling And Omics-Integrative Analysis Of Single And Multi-Organism Systems: Discovery And Redesign, Mohammad Mazharul Islam

Chemical & Biomolecular Engineering Theses, Dissertations, & Student Research

Computations and modeling have emerged as indispensable tools that drive the process of understanding, discovery, and redesign of biological systems. With the accelerating pace of genome sequencing and annotation information generation, the development of computational pipelines for the rapid reconstruction of high-quality genome-scale metabolic networks has received significant attention. These models provide a rich tapestry for computational tools to quantitatively assess the metabolic phenotypes for various systems-level studies and to develop engineering interventions at the DNA, RNA, or enzymatic level by careful tuning in the biophysical modeling frameworks. in silico genome-scale metabolic modeling algorithms based on the concept of optimization ...


Degumming Of Hemp Fibers Using Combined Microwave Energy And Deep Eutectic Solvent, Bulbul Ahmed 2021 Louisiana State University and Agricultural and Mechanical College

Degumming Of Hemp Fibers Using Combined Microwave Energy And Deep Eutectic Solvent, Bulbul Ahmed

LSU Master's Theses

Hemp is considered as one of the sustainable agricultural fiber materials. Degumming or surface modification of hemp bast is needed to produce single fibers for ensuing textile and industrial applications. The traditional degumming process necessitates a high amount of alkali, which causes detrimental environmental pollution. This study offers a new method to degum hemp fibers with reduced use of harmful alkali and precious water resources. In this work, hemp bast fibers were degummed by using combined microwave energy and deep eutectic solvent (DES). The properties of hemp fibers manufactured by this method were investigated and compared with the traditional alkali ...


Novel Approaches Towards Improved Purity In High Yield Transcription Reactions, Elvan Cavac 2021 University of Massachusetts Amherst

Novel Approaches Towards Improved Purity In High Yield Transcription Reactions, Elvan Cavac

Doctoral Dissertations

High yields of RNA (e.g., mRNA, gRNA, lncRNA) are routinely prepared following a two-step approach: high yield in vitro transcription using T7 RNA polymerase, followed by extensive purification using gel or chromatic methods. In high yield transcription reactions, as RNA accumulates in solution, T7 RNA polymerase rebinds and extends the encoded RNA (using the RNA as a template), resulting in a product pool contaminated with longer than desired, (partially) double stranded impurities. Current purification methods often fail to fully eliminate these impurities which, if present in therapeutics, can stimulate the innate immune response with potentially fatal consequences. This study ...


Creation And Application Of Various Tools For The Reconstruction, Curation, And Analysis Of Genome-Scale Models Of Metabolism, Wheaton L. Schroeder 2021 University of Nebraska - Lincoln

Creation And Application Of Various Tools For The Reconstruction, Curation, And Analysis Of Genome-Scale Models Of Metabolism, Wheaton L. Schroeder

Chemical & Biomolecular Engineering Theses, Dissertations, & Student Research

Systems biology uses mathematics tools, modeling, and analysis for holistic understanding and design of biological systems, allowing the investigation of metabolism and the generation of actionable hypotheses based on model analyses. Detailed here are several systems biology tools for model reconstruction, curation, analysis, and application through synthetic biology. The first, OptFill, is a holistic (whole model) and conservative (minimizing change) tool to aid in genome-scale model (GSM) reconstructions by filling metabolic gaps caused by lack of system knowledge. This is accomplished through Mixed Integer Linear Programming (MILP), one step of which may also be independently used as an additional curation ...


Effect Of Developer Temperature On Photoresist Contrast In Grayscale Lithography, Dale Farnan, George Patrick Watson 2021 Singh Center for Nanotechnology

Effect Of Developer Temperature On Photoresist Contrast In Grayscale Lithography, Dale Farnan, George Patrick Watson

Protocols and Reports

SPR 220-3 photoresist was spin-coated onto a silicon wafer, exposed using a Heidelberg DWL66+ laserwriter at different laser powers, and developed at different temperatures. The effect of developer temperature on photoresist contrast was examined. Results show that increasing developer temperature decreased photoresist contrast and increased required dose.


Exploration Of The Sludge Biodiesel Pathway, Zachary Christman 2021 University of Nebraska - Lincoln

Exploration Of The Sludge Biodiesel Pathway, Zachary Christman

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Wastewater sludge is an overlooked source of fat, oil, and grease (FOG) that could be converted into biodiesel. The United States produces about 8 million tons of sludge per year. The disposal cost for this amount of sludge is about 2 billion dollars. The widespread availability and low cost of sludge compared to other biodiesel raw materials make it an economical choice for a renewable fuel. Using sludge as a raw material can produce 25 to 30 mg per gram of fatty acid methyl ester (FAME); the main component of biodiesel. Sludge biodiesel has the potential of transforming a portion ...


Investigative Study Of Microalgal And Electrochemical Wastewater Treatment Systems And Modeling Of The Wafer-Enhanced Electrodeionization Using Supervised Learning, Humeyra Betul Ulusoy Erol 2021 University of Arkansas, Fayetteville

Investigative Study Of Microalgal And Electrochemical Wastewater Treatment Systems And Modeling Of The Wafer-Enhanced Electrodeionization Using Supervised Learning, Humeyra Betul Ulusoy Erol

Graduate Theses and Dissertations

Wastewater has a serious impact on environment and public health due to its high concentration of nutrients and toxic contaminants. Without proper treatment, excess nutrients discharged in wastewater can cause a damage to the ecosystem such as undesirable pH shifts, cyanotoxin production, and low dissolved oxygen concentrations.

Main objectives of this dissertation work were to investigate i) the biofuel potential of P. cruentum when grown in swine wastewater, ii) the influence of four most commonly used ion exchange resins on the system efficiency and selectivity for the removal of sodium, calcium, and magnesium ions, and iii) the modeling of wafer-enhanced ...


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