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Ethanol To Ethyl Acetate, Kerri Heuser, Vinson Liao, Neha Narain 2019 University of Pennsylvania

Ethanol To Ethyl Acetate, Kerri Heuser, Vinson Liao, Neha Narain

Senior Design Reports (CBE)

Ethyl acetate is a specialty chemical with dominant use as a solvent, and total production exceeding 3.7MMT annually in 2017. Current production methods mainly utilize petrochemical-derived feedstocks of ethanol and acetic acid that undergo Fischer Esterification to produce ethyl acetate. This is traditionally considered to be an unsustainable process. A new startup company, Greenyug, based out of Santa Barbara, CA, has proposed a new design that solely utilizes fermentation ethanol, and, as a result, is considered sustainable. Greenyug describes (in accordance to US Patents US8562921B2, US9079851B2, and US9447018B2) a two-step reaction pathway that first partially dehydrogenates ethanol to acetaldehyde ...


Culture-Derived Human Platelets For Clinical Transfusion, Alayna Choo, Gabrielle Leon, Susanna Jaramillo 2019 University of Pennsylvania

Culture-Derived Human Platelets For Clinical Transfusion, Alayna Choo, Gabrielle Leon, Susanna Jaramillo

Senior Design Reports (CBE)

Platelets are a vital component of human blood due to their role in clotting. Despite the incredible importance of platelets for human survival, there is very little supply of platelets to meet the clinical demand. Currently, platelets are only supplied from donations, whether through whole blood or apheresis. Therefore, there is a need for ex vivo means of producing platelets at a price that is competitive with the current market price. This project seeks to meet 65% of the demand for platelets in Philadelphia by producing platelets ex vivo in a process that utilizes shear stress to induce platelet formation ...


Process For Sustainably Sourced 2,5 Furan Dicarboxylic Acid, Michael Bell, David Gutierrez, Kevin Wang 2019 University of Pennsylvania

Process For Sustainably Sourced 2,5 Furan Dicarboxylic Acid, Michael Bell, David Gutierrez, Kevin Wang

Senior Design Reports (CBE)

Currently, there is a significant market for terephthalic acid (TPA)--a precursor for the ubiquitous plastic polymer polyethylene terephthalate (PET). As consumer preferences swing to more environmentally-friendly products, sustainable plastics, such as polyethylene furanoate (PEF), become more widely desired. In this project, the authors suggest a process for the synthesis of 2,5-Furandicarboxylic acid (FDCA), a diacid used in the synthesis of PEF, through a multi-step procedure following current industrial practices for TPA oxidation and patents from three sources: Synvina, a joint venture between Archer Daniels Midland (ADM) Company and DuPont Industrial Biosciences (DuPont), and Eastman. The first step of ...


Chemical Recycling Of Plastics By Dissolution, Pensiri Naviroj, James Treacy, Conrad Urffer 2019 University of Pennsylvania

Chemical Recycling Of Plastics By Dissolution, Pensiri Naviroj, James Treacy, Conrad Urffer

Senior Design Reports (CBE)

There is significant upside potential for the recycling of plastic wastes, especially for waste polyethylene plastic bags. Plastic containers and packaging have the most tonnage of waste plastics sent to landfills, and there are limited number of communities collecting single-use polyethylene plastics bags for recycling. This project explores the dissolution-precipitation technique as a viable plastics recycling method. Beginning with a feed assumed to be primarily low density polyethylene (LDPE), plastic bag waste is cleaned and 50 tons per day of this cleaned LDPE waste enters the main process. The proposed design includes a dissolution and precipitation vessel. The design is ...


Production Of Single Cell Protein And Astaxanthin Using Methanol As Carbon Source, Corinne Pillarella, Craig Jahnke, Brielle Weiner 2019 University of Pennsylvania

Production Of Single Cell Protein And Astaxanthin Using Methanol As Carbon Source, Corinne Pillarella, Craig Jahnke, Brielle Weiner

Senior Design Reports (CBE)

Singlecell protein (SCP) is the biomass of unicellular organisms, such as bacteria or yeast, which is used commonly as a food source for animals. With a high protein content, a broad amino acid profile, and the ability to produce essential organic compounds and vitamins, SCP is a promising alternative to other classical sources of animal feed. Several processes have been developed to manufacture SCP for use in feedstocks for the sustainable farming of fish and other aquatic life, or aquaculture, which is one of the fastest growing food markets in the world. Here, a process is presented for the production ...


Conversion Of Omega-3 Fatty Acids From Algae Biomass Produced Biodiesel, Matthew Peters, Jacquelyn Stokes, Ryan Tu 2019 University of Pennsylvania

Conversion Of Omega-3 Fatty Acids From Algae Biomass Produced Biodiesel, Matthew Peters, Jacquelyn Stokes, Ryan Tu

Senior Design Reports (CBE)

As the effects of global warming continue to escalate, carbon-neutral fuels are becoming sought after alternatives to traditional fossil fuels. Biodiesel is a promising source of carbon-neutral fuel that can be produced from organisms such as algae. A new process has been proposed by Yadav for algal oil extraction using sonic waves and carbon dioxide microbubbles (Yadav et al., 2019). Although a novel idea, it currently struggles to compete economically with other types of biodiesel production as well as traditional food sources. However, a small subsection of the FAMEs is able to be further processed into Omega-3 fatty acids. These ...


Synthesis Of Green Hydrocarbons Using The Air To Fuels™ Technology, Tyler Durkin, Kyle Kersey, James Paolini 2019 University of Pennsylvania

Synthesis Of Green Hydrocarbons Using The Air To Fuels™ Technology, Tyler Durkin, Kyle Kersey, James Paolini

Senior Design Reports (CBE)

Our AIR TO FUELS™ project is based off a process designed by Carbon Engineering by the same name which seeks to synthesize liquid fuels from capture carbon dioxide captured from the atmosphere. The need for reduced greenhouse gases in our atmosphere is more urgent than ever and becoming more dire by the day. Still, our society’s reliance on liquid fuels like gasoline, diesel, and jet fuel is deeply rooted in our infrastructure and certainly not changing quickly or soon. The AIR TO FUELS™ process seeks to tackle both of these problems by taking carbon dioxide out of the atmosphere ...


Future Api Manufacturing Excellence, Emily Cunningham, Rachel Wilson, Marc Geagea 2019 University of Pennsylvania

Future Api Manufacturing Excellence, Emily Cunningham, Rachel Wilson, Marc Geagea

Senior Design Reports (CBE)

Many pharmaceutical manufacturing processes are costly, time-intensive, and energy-intensive. Due to the high operational costs, optimization of these processes would result in large economic savings. Fluid bed granulation takes inputs of air temperature, air flow rate, phase duration, binder spray rate, and inlet air humidity. A series of simulations were run to determine optimal operating conditions. It was determined that the process parameters should be limited to the following to meet product standards and reduce costs: air flow rate of 2800 (m3/h), inlet air temperature of 55˚C for spraying and 75˚C for drying, phase duration 124 ...


Green Glycol: A Novel 2-Step Process, Ingemar Falcones, Sarah Golden, Maria Kowalchuk 2019 University of Pennsylvania

Green Glycol: A Novel 2-Step Process, Ingemar Falcones, Sarah Golden, Maria Kowalchuk

Senior Design Reports (CBE)

Ethylene glycol demand is growing rapidly, particularly in the global polyethylene terephthalate markets.¹ Traditional production of non-renewable ethylene glycol involves steam cracking of ethane or the methanol-to-olefin process to obtain ethylene.6 In response to environmental movements, Coca-Cola® began creating ethylene glycol from renewably sourced ethanol, by producing the ethylene oxide intermediate in a two-step reaction process.² Novel research at Leiden University, entitled Direct conversion of ethanol into ethylene oxide on gold based catalysts, explores a catalyst which produces ethylene oxide in one step, showing potential for a more efficient renewable process.³ This project explores the scaling ...


Phylogenetic And Experimental Characterization Of An Acyl-Acp Thioesterase Family Reveals Significant Diversity In Enzymatic Specificity And Activity, Fuyuan Jing, David C. Cantu, Jarmila Tvaruzkova, Jay P. Chipman, Basil J. Nikolau, Marna D. Yandeau-Nelson, Peter J. Reilly 2019 Iowa State University

Phylogenetic And Experimental Characterization Of An Acyl-Acp Thioesterase Family Reveals Significant Diversity In Enzymatic Specificity And Activity, Fuyuan Jing, David C. Cantu, Jarmila Tvaruzkova, Jay P. Chipman, Basil J. Nikolau, Marna D. Yandeau-Nelson, Peter J. Reilly

Marna Yandeau-Nelson

Background: Acyl-acyl carrier protein thioesterases (acyl-ACP TEs) catalyze the hydrolysis of the thioester bond that links the acyl chain to the sulfhydryl group of the phosphopantetheine prosthetic group of ACP. This reaction terminates acyl chain elongation of fatty acid biosynthesis, and in plant seeds it is the biochemical determinant of the fatty acid compositions of storage lipids.

Results: To explore acyl-ACP TE diversity and to identify novel acyl ACP-TEs, 31 acyl-ACP TEs from wide-ranging phylogenetic sources were characterized to ascertain their in vivo activities and substrate specificities. These acylACP TEs were chosen by two different approaches: 1) 24 TEs were ...


A Review Of Dissolved Oxygen Concentration Measurement Methods For Biological Fermentations, Samuel T. Jones, Theodore J. Heindel 2019 Iowa State University

A Review Of Dissolved Oxygen Concentration Measurement Methods For Biological Fermentations, Samuel T. Jones, Theodore J. Heindel

Theodore J. Heindel

Dissolved oxygen levels in biological processes depend on the biological, chemical, and physical properties of the process being monitored. The analysis of dissolved oxygen concentration is a key test for process control and optimization. A review of the measurement methods for dissolved oxygen concentrations will be presented in this paper. Included in this review are the chemical, volumetric, tubing, electrochemical electrode, and optode methods. Advantages and disadvantages of these methods are discussed and key considerations for their use are summarized.


The Influence Of External Airlift Loop Bioreactor Configuration On Bioreactor Hydrodynamics, Samuel T. Jones, Theodore J. Heindel 2019 Iowa State University

The Influence Of External Airlift Loop Bioreactor Configuration On Bioreactor Hydrodynamics, Samuel T. Jones, Theodore J. Heindel

Theodore J. Heindel

Airlift bioreactors have the potential of being used in many fermentation processes, and understanding their hydrodynamics is key to their use. To this end, gas holdup (i.e., volumetric gas fraction) and superficial liquid velocity in the downcomer and riser are studied in an external loop airlift bioreactor with an area ratio of 1:16. Two downcomer configurations are investigated consisting of the downcomer open or closed to the atmosphere. Experiments for these two configurations are carried out, over a range of superficial gas velocities from UG = 0.5 to 20 cm/s, using three aeration plates with open area ...


Understanding Conformational Regulation Of The Integrin I-Domain For Design Of Chimeric Protein Switches, Niall Phelan Terry, Liang Fang, J Vincent Price, Eric T. Boder Dr. 2019 University of Tennessee, Knoxville

Understanding Conformational Regulation Of The Integrin I-Domain For Design Of Chimeric Protein Switches, Niall Phelan Terry, Liang Fang, J Vincent Price, Eric T. Boder Dr.

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Within all complex biological processes intricate proteins are expressed to complete every niche and necessary task. Many express multiple allosterically regulated conformational states, with protein function regulated by effector molecules and other ligands. One such protein is the LFA-1 surface integrin protein and its inserted domain, the I-domain. We Isolated the I-domain for investigation of determining binding properties and understanding conformational regulations of affinity changes to its target ligand ICAM-1, for further use in chimeric protein switch design. A large change in binding affinity was found through the deletion of a sub-sequence of amino acids in I-domain known as the ...


Elucidating Growth Coupling Of Metabolites In E. Coli And Characterizing Modularity., Preston N. Nicely 2019 University of Tennessee, Knoxville

Elucidating Growth Coupling Of Metabolites In E. Coli And Characterizing Modularity., Preston N. Nicely

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

This project seeks to increase the efficiency of rapid engineering strain design by elucidating the functionality of a modular cell. The modular cell theory insinuates that there exists a baseline cellular framework from which cellular products can be produced by inserting a designed production module into the cell. In this study, recombinant gene cloning techniques were used to design metabolite production modules that focused on increasing the production, titer, and yield of ethanol and lactate within Escherichia coli (E. coli). An auxotrophic chassis cell was created by ModCell2 and multi-objective strain design to determine the genes necessary for deletion. The ...


Design Of A Portable Biogas Purification And Storage System, Anikumar Kosna, Bade Shrestha 2019 Western Michigan University

Design Of A Portable Biogas Purification And Storage System, Anikumar Kosna, Bade Shrestha

Faculty Research and Creative Activities Award (FRACAA) Recipients

The objective of this study is to propose and design a commercial portable purification and storage system to commercialize biogas (which mainly consists of 45 - 75 % of methane and 15 - 16% of carbon dioxide), produced from household/non-commercial biogas plants. The selection of purification process is based on the portability of the purification system.


Amphiphilic Polyanhydride-Based Recombinant Muc4Β-Nanovaccine Activates Dendritic Cells, Kasturi Banerjee, Shailendra K. Gautam, Prakash Kshirsagar, Kathleen A. Ross, Gaelle Spagnol, Paul Sorgen, Michael J. Wannemeuhler, Balaji Narasimhan, Joyce C. Solheim, Sushil Kumar, Surinder K. Batra, Maneesh Jain 2019 University of Nebraska Medical Center

Amphiphilic Polyanhydride-Based Recombinant Muc4Β-Nanovaccine Activates Dendritic Cells, Kasturi Banerjee, Shailendra K. Gautam, Prakash Kshirsagar, Kathleen A. Ross, Gaelle Spagnol, Paul Sorgen, Michael J. Wannemeuhler, Balaji Narasimhan, Joyce C. Solheim, Sushil Kumar, Surinder K. Batra, Maneesh Jain

Chemical and Biological Engineering Publications

Mucin 4 (MUC4) is a high molecular weight glycoprotein that is differentially overexpressed in pancreatic cancer (PC), functionally contributes to disease progression, and correlates with poor survival. Further, due to its aberrant glycosylation and extensive splicing, MUC4 is a potential target for cancer immunotherapy. Our previous studies have demonstrated the utility of amphiphilic polyanhydride nanoparticles as a useful platform for the development of protein-based prophylactic and therapeutic vaccines. In the present study, we encapsulated purified recombinant human MUC4-beta (MUC4β) protein in polyanhydride (20:80 CPTEG:CPH) nanoparticles (MUC4β-nanovaccine) and evaluated its ability to activate dendritic cells and induce adaptive immunity ...


Analyzing Drug Release Kinetics From Water-Soluble Polymers, Sean M. Kelly, Arun K. Upadhyay, Akash Mitra, Balaji Narasimhan 2019 Iowa State University

Analyzing Drug Release Kinetics From Water-Soluble Polymers, Sean M. Kelly, Arun K. Upadhyay, Akash Mitra, Balaji Narasimhan

Chemical and Biological Engineering Publications

The ability to develop predictive mathematical models of therapeutic release from pharmaceutical formulations has enormous potential to enhance our understanding of such systems and improve the controlled release of the payload. The current work describes the development and testing of a one-dimensional model of drug transport from amorphous, swelling/dissolving polymers. Model parameters such as the diffusivities of water and drug, the initial loading of the drug, the polymer dissolution rate, drug-polymer interactions, and the tablet thickness were varied, demonstrating the ability to tune the release to be controlled by either drug diffusion or polymer chain disentanglement. In addition, predictions ...


Enhancing The Co-Utilization Of Biomass-Derived Mixed Sugars By Yeasts, Meirong Gao, Deon Ploessl, Zengyi Shao 2019 Iowa State University

Enhancing The Co-Utilization Of Biomass-Derived Mixed Sugars By Yeasts, Meirong Gao, Deon Ploessl, Zengyi Shao

Chemical and Biological Engineering Publications

Plant biomass is a promising carbon source for producing value-added chemicals, including transportation biofuels, polymer precursors, and various additives. Most engineered microbial hosts and a select group of wild-type species can metabolize mixed sugars including oligosaccharides, hexoses, and pentoses that are hydrolyzed from plant biomass. However, most of these microorganisms consume glucose preferentially to non-glucose sugars through mechanisms generally defined as carbon catabolite repression. The current lack of simultaneous mixed-sugar utilization limits achievable titers, yields, and productivities. Therefore, the development of microbial platforms capable of fermenting mixed sugars simultaneously from biomass hydrolysates is essential for economical industry-scale production, particularly for ...


Single Enzyme Synthesis And Bio-Imitation Of Functional Nanocrystals, Abdolhamid Sadeghnejad 2019 Lehigh University

Single Enzyme Synthesis And Bio-Imitation Of Functional Nanocrystals, Abdolhamid Sadeghnejad

Theses and Dissertations

Green synthesis methods of inorganic materials have gained lots of attention due to lower pollution and toxic precursors during the synthesis. One of the modern green methods of synthesis is biosynthesis in which a DNA, small peptide molecule, or amino acids plays the main role of synthesis. Biomineralization, the biosynthesis process happens in the nature by elements, has been considered as a green, low cost, and scalable method of synthesis. In comparison with conventional synthesis methods, biomineralization happens under ambient conditions in aqueous phase that can be applied for synthesis of inorganic nanostructure metal sulfide semiconductors gained interest due to ...


The Endocytotic Fate Of A Mesoporous Silica Nanoparticle Supported Lipid Bylayer Crispr Delivery Vehicle, Angelea Maestas-Olguin 2019 University of New Mexico - Main Campus

The Endocytotic Fate Of A Mesoporous Silica Nanoparticle Supported Lipid Bylayer Crispr Delivery Vehicle, Angelea Maestas-Olguin

2019 Award Winners

No abstract provided.


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