Food Science Commons^™

Trends And Applications Of Food Protein-Origin Hydrolysates And Bioactive Peptides, Yi-Hsieng Samuel Wu, Yi-Chen Chen

Journal of Food and Drug Analysis

It was reported that protein hydrolysates or derived peptides have more functionalities than their parent protein. Most functional protein hydrolysates or peptides are identified from various food products, including plant, fish, and land-animal protein sources. Within a few decades, the application of food protein-origin functional hydrolysates or peptides could be divided into two main categories according to their applied intentions: 1) preservatives and bioactive packing materials; 2) nutraceutical ingredients. According to the literature, the applications of food protein-origin functional hydrolysates or peptides on food preservative and nutraceutical ingredients have attracted much attention. However, the approach method should be changed. Multi-activities, …

A Study On Plant-Derived Small Proteins And Peptides With Antiyeast Activity And Preliminary Investigations In Food Materials, Giulia Mignone

Theses

The discovery of novel antimicrobial molecules would benefit considerably different biotechnological fields, including medicine, agriculture and the food industry, on which this thesis work focuses. Specifically, here are presented fundamental studies on plant-derived antimicrobial proteins for the reduction of food spoilage caused by yeast microorganisms. Small proteins and peptides from the plant innate immune system possess several characteristics which could make them ideal candidates for a novel and natural antispoilage agent. The first Chapter reviews proteins families associated with the plant innate immune system that exhibit antiyeast activity and low molecular weight (<30 kDa), focusing on their structure and antiyeast mode of action. Chapter 2 investigates two peptides, Pn-AMP1 and Pn-AMP2 belonging to the hevein-type peptides family; peptides were synthetically generated using their native amino acid sequence. Synthetic Pn-AMP1 showed fast action activity against common food spoilage yeasts, it resulted potentially safe for consumption, and it was successfully incorporated in UHT milk and Fanta Orange, where it contained the growth of Kluyveromyces lactis and Zygosaccharomyces bailii, respectively. A different family of antimicrobial plant proteins (2S albumins) was studied in Chapters 3, 4, 5 and 6. Chapter 3 describes the selective extraction of an antiyeast protein (labelled WMS1 in this thesis work) of ~14 kDa from white mustard (Brassica hirta) seeds. The protein was purified in a relatively high yield, using a simple protocol that included only one chromatographic method step. In Chapter 4, bioinformatics tools and wet-lab techniques are used to study the structure and classify the antiyeast protein. The WMS1 antiyeast protein was identified as an isoform of the Napin protein Allergen Sin a 1 belonging to the 2S albumins family, characterised by high structural stability which donates to the protein resistance heat treatment and proteolytic degradation. Chapter 5 describes the antimicrobial spectrum of WMS1 , its antiyeast mechanism of action and its validity as preservative agent. Although WMS1 resulted potentially safe for consumption and inhibited the growth of Z. bailii in several beverage systems, its biotechnological potential is limited by its allergic properties. In Chapter 6, the protein WMS1 was cloned in a pET28a-MBP vector and expressed in an E. coli BL21 system. Interestedly, recombinant WMS1 and native WMS1 showed similar activity against the yeast Z. bailii. Thesis abstract 2 Chapter 7 includes a protocol for the rational design of ultra-short antimicrobial peptides. Since ultra-short antimicrobial sequences can be found within the sequence of any larger protein, the protocol was applied using WMS1 primary sequence, and seven ultra-short SinA-AMPs were chemically generated. Among the various SinA-AMPs, the peptide labelled SinA-pepIII had in vitro activity against several common yeast spoilage strains via membrane permeabilisation and it could be employed as a food preservative as it was pH- and heat- stable, potentially safe in terms of human consumption and it prevented the growth of Z. bailii in cranberry juice. The results obtained in this work offer new perspectives in the research for novel and clean-label food preservatives to fight the food waste phenomenon.

The Effects Of Vitamin D Supplementation On Athletic Performance And Injury Prevention, Amani Adeeb Abushamma

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Vitamin D supplementation has numerous effects on athletic performance and plays a significant role in preventing an athlete’s risk of getting injuries. Vitamin D has an impact on numerous physiological functions such as: bone health, muscle function, inflammatory response, and immune function. An athlete’s bone and muscle health are essential for maximum performance and career success. A bone fracture due to vitamin D deficiency can delay an athlete’s training and ultimately inhibit obtaining a collegiate scholarship and/or contract. A cross sectional study found that more than half of athletic trainers did not view 25-hydroxyvitamin D(25[OH]) testing and vitamin D supplementation …

The Mosaic Oat Genome Gives Insights Into A Uniquely Healthy Cereal Crop, Nadia Kamal, Nikos Tsardakas Renhuldt, Johan Bentzer, Heidrun Gundlach, Georg Haberer, Angéla Juhász, Thomas Lux, Utpal Bose, Jason A. Tye-Din, Daniel Lang, Nico Van Gessel, Ralf Reski, Yong Bi Fu, Peter Spégel, Alf Ceplitis, Axel Himmelbach, Amanda J. Waters, Wubishet A. Bekele, Michelle L. Colgrave, Mats Hansson, Nils Stein, Klaus F.X. Mayer, Eric N. Jellen, Peter J. Maughan, Nicholas A. Tinker, Martin Mascher, Olof Olsson, Manuel Spannagl, Nick Sirijovski

Research outputs 2022 to 2026

Cultivated oat (Avena sativa L.) is an allohexaploid (AACCDD, 2n = 6x = 42) thought to have been domesticated more than 3,000 years ago while growing as a weed in wheat, emmer and barley fields in Anatolia1,2. Oat has a low carbon footprint, substantial health benefits and the potential to replace animal-based food products. However, the lack of a fully annotated reference genome has hampered efforts to deconvolute its complex evolutionary history and functional gene dynamics. Here we present a high-quality reference genome of A. sativa and close relatives of its diploid (Avena longiglumis, AA, 2n = 14) and tetraploid …

Characterization Of Severity In Zellweger Spectrum Disorder By Clinical Findings: A Scoping Review, Meta-Analysis And Medical Chart Review, Mousumi Bose, Christine Yergeau, Yasmin D’Souza, David D. Cuthbertson, Melisa J. Lopez, Alyssa K. Smolen, Nancy E. Braverman

Department of Nutrition and Food Studies Scholarship and Creative Works

Zellweger spectrum disorder (ZSD) is a rare, debilitating genetic disorder of peroxisome biogenesis that affects multiple organ systems and presents with broad clinical heterogeneity. Al-though severe, intermediate, and mild forms of ZSD have been described, these designations are often arbitrary, presenting difficulty in understanding individual prognosis and treatment effectiveness. The purpose of this study is to conduct a scoping review and meta-analysis of existing literature and a medical chart review to determine if characterization of clinical findings can predict severity in ZSD. Our PubMed search for articles describing severity, clinical findings, and survival in ZSD resulted in 107 studies (representing …

Physicochemical, Techno-Functional And Bioactive Properties Of Camel Whey Protein Concentrate As Affected By Spray-Drying And Ultrasonication Treatment, Alanoud Muhsen Al Thaibani

Theses

Whey protein concentrates (WPCs) are gaining importance as a functional ingredient due to their nutritional, techno-functional and bioactive properties. In this study, WPCs were prepared from skimmed camel milk, then either spray-dried (SD) at 170, 185 and 200°C, or treated by ultrasonication (US) (20 kHz) for 5, 10 and 15 min followed by freeze-drying to obtain powders. The characterization of WPC powders was carried out by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Reverse-Phase Ultra Performance Liquid Chromatography (RP-UPLC) which showed that the US treatment degraded the proteins more than SD. The morphology, particle size, and surface charge of WPC …

The Effects Of Cofermentation Of Cider And Apple Pomace On Cider Attributes, Abigail D. Affonso

Master's Theses

Phenolics are critical to the sensory attributes and health benefits of hard cider due to their contribution to the flavor, mouthfeel, and antioxidant activity. With the increase in demand for cider, the use of dessert apple varieties has become more common leaving ciders lacking in phenolics. However, a promising method to increase their phenolic content is through maceration with apple pomace. This study evaluated the effect maceration with apple pomace during cider fermentation on the extraction of phenolic compounds, as well as its effect on the sensory properties of the final product. For this study, ciders were fermented with 0% …

The Effect Of Reduced Cp, Synthetic Amino Acid Supplemented Diets On Growth Performance And Nutrient Excretion In Wean To Finish Swine, Caitlin E. Vonderohe, Kayla M. Mills, Shule Liu, Matthew D. Asmus, Emily R. Otto-Tice, Brian T. Richert, Ji-Qin Ni, John Scott Radcliffe

Animal and Food Sciences Faculty Publications

Mixed sex pigs (n = 720) were placed in 12 rooms (Purdue Swine Environmental Research Building) to measure the effect of reduced crude protein (CP), amino acid (AA)-supplemented diets on growth and the carcass. Pigs were blocked by body weight (BW) and gender and allotted to room and pen (10 mixed-sex pigs/pen). Pigs were fed a nine-phase, wean-finish program. Control pigs consumed corn-soybean meal-distiller’s dried grains with solubles (DDGS) diets containing no to minimal (Met) synthetic AA. The 2X diet was formulated to meet the seventh most-limiting AA, and balanced using synthetic AAs to meet all AA needs. The 1X …

High Pressure Processing Of Cashew Milk, Rachel Coggins

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Plant-based foods are becoming increasingly popular because of changing consumer preferences. Most plant-based milks are not a direct replacement for cow’s milk because they do not have the same nutritional value and physicochemical properties. However, these properties could be modified and enhanced through processing. For example, high pressure processing (HPP) can affect irreversibly protein structure and functionality and potentially enhance enzymatic hydrolysis. HPP is used widely in the beverage industry for cold pasteurization. In this study, the effects of HPP treatment on (a) the inactivation of Listeria innocua, a commonly used surrogate for Listeria monocytogenes in HPP research, in …

A Cross-Country Investigation Of Social Image Motivation And Acceptance Of Lab-Grown Meat In Singapore And The United States, Mark Chong, Angela K. Y. Leung, Verity Lua

Research Collection Lee Kong Chian School Of Business

This research has three goals. First, it sets out to compare consumer acceptance of lab-grown meat in the U.S. and in Singapore. Second, it seeks to explain the difference in Americans' and Singaporeans' acceptance of lab-grown meat by examining their eating motivations. Specifically, we focused on social image motivations – the motivations to present oneself positively in social contexts. Third, this study also aims to assess if exposure to information about lab-grown meat communicated by celebrity versus expert social media influencers (SMIs) can impact people's acceptance of lab-grown meat products. Our analysis showed that Singaporean participants had greater acceptance of …

Oxidative Stress Protection By Canary Seed (Phalaris Canariensis L.) Peptides In Caco-2 Cells And Caenorhabditis Elegans, Uriel Urbizo-Reyes, Kee-Hong Kim, Lavanya Reddivari, Joseph M. Anderson

Department of Food Science Faculty Publications

During oxidative stress, degenerative diseases such as atherosclerosis, Alzheimer's, and certain cancers are likely to develop. Recent research on canary seed (Phalaris canariensis) peptides has demonstrated the high in vitro antioxidant potential. Thus, this study aimed to assess the cellular and in vivo antioxidant capacity of a low-molecular-weight (<3 kDa) canary seed peptide fraction (CSPF) using Caco-2 cells and the Caenorhabditis elegans model. The results show that the CSPF had no cytotoxicity effect on Caco-2 cells at any tested concentration (0.3-2.5 mg/mL). Additionally, the cellular antioxidant activity (CAA) of the CSPF was concentration-dependent, and the highest activity achieved was 80% by the CSPF at 2.5 mg/mL. Similarly, incubation with …

Multifunctional Bioactive Properties Of Plant-Based Protein Hydrolysates Derived From Citrullus Colocynthis (Bitter Apple) And Pennisetum Glaucum (Pearl Millet) Seeds, Munira Jassem Alblooshi

Theses

Protein hydrolysates (PH) from plant-based proteins (PBP) are gaining importance due to the availability, sustainable and economical production when compared to their an-imal origin counterpart. Therefore, this study explored under-utilized PBP hydroly-sates derived from Bitter apple (BA) and Pearl millet (PM) and determined their bio-active properties. Enzymatic hydrolysis (EH) of BA and PM proteins was carried out by enzymes such as alcalase (A), bromelain (B), and chymotrypsin (C) for 3, 6, and 9 h to produce PH possessing high bioactive properties. Highest degree of hydrolysis (DH) was reported in BAPH generated with C9 (87.8%) whereas for PMPH highest DH was …

Inflammatory Diseases And Resections Of The Digestive Tract Influence The Risk Of Circulating Food-Specific-Igg, Jacques Izard, Walker Carson, Joseph Baumert, Jennifer Clarke

Department of Food Science and Technology: Faculty Publications

Objectives: Individuals affected with inflammatory diseases of the digestive tract or have had surgical removal of sections of the digestive tract are often in need to adjust their diet. The symptomatology associated with food intake issues is often informally reported as food sensitivity. We investigated the circulating food-specific-IgG among ten conditions.

Conclusion: The risk of the having circulating food-specific IgG differed widely among the conditions investigated. The maintenance of a colonic environment might influence the risk of food sensitivity in ostomates.

Effect Of Vine Age And Selected Winemaking Techniques On The Sensory Profile Of Central Coast Red Wines, Isabelle Lomonaco

Master's Theses

Descriptive sensory analysis is essential to understanding how differing varietals, viticultural techniques, enological techniques, vintage, regions, etc. impact the sensory characteristics found in wines. These studies analyzed the sensory impacts of vine age, whole cluster and stem addition influences, the frequency of cap management regimes, and selected fermentation variables, on 2019 Zinfandel, 2020 Syrah, 2020 Tannat, 2020 Cabernet Sauvignon, and 2021 Graciano wines respectively, from the Central Coast of California, USA. The investigation of Old vine Zinfandel indicated that these wines were defined as complex due to the wide variety of descriptors used to characterize them, having high color saturation …

Producing Bacterial Cellulose From Coffee (Coffea Arabica) Kombucha Fermentation And Assessment Of Its Efficacy To Remove Pathogenic Bacteria From Contaminated Water And A Pollutant Dye From Aqueous Solution, Federico Bueno

LSU Doctoral Dissertations

Bacterial cellulose (BC), composed of cellulosic nanofibrils, is synthesized by a symbiotic culture of bacteria and yeast during the fermentation of coffee kombucha (CK). BC may be used as a purification agent and adsorbent. The overall goal of this project was to synthesize BC from CK fermentation and assess its efficacy in removing pathogenic bacteria from contaminated water and a pollutant dye from aqueous solution. The objectives of this study were to: (1) compare BCs that were produced from different carbohydrate substrates in the fermentation of CK, (2) characterize dried BCs produced using various drying methods, (3) evaluate BC as …

A Classification System For Defining And Estimating Dietary Intake Of Live Microbes In Us Adults And Children, Maria L. Marco, Robert Hutkins, Colin Hill, Victor L. Fulgoni Iii, Christopher J. Cifelli, Jaime Gahche, Joanne L. Slavin, Daniel Merenstein, Daniel J. Tancredi, Mary E. Sanders

Department of Food Science and Technology: Faculty Publications

Background:

Consuming livemicrobes in foods may benefit human health. Live microbe estimates have not previously been associated with individual foods in dietary databases.

Objectives:

We aimed to estimate intake of live microbes in US children (aged 2–18 y) and adults (≥19 y) (n = 74,466; 51.2% female).

Methods:

Using cross-sectional data from the NHANES (2001–2018), experts assigned foods an estimated level of live microbes per gram [low (Lo), <10⁴ CFU/g; medium (Med), 10⁴–10⁷ CFU/g; or high (Hi), >10⁷ CFU/g]. Probiotic dietary supplements were also assessed. The mean intake of each live microbe …

Becky Landes, Becky Landes, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Becky Landes is a current Food Service Director for Manchester Community Schools in Indiana. For over 20 years, Becky has been in Child Nutrition. She holds a Level 4 Certificate from the School Nutrition Association and is pursuing a Bachelor’s degree in Business Administration and Management. Becky is involved in Farm to School in Indiana. She has collaborated with the Indiana Department of Education, Indiana Grown for Schools, and various other stakeholders in procuring and serving local foods in schools. Becky likes to be involved in the many facets of Child Nutrition. She participates in and is a member of …

Large-Scale Analyses Of Heat Shock Transcription Factors And Database Construction Based On Whole-Genome Genes In Horticultural And Representative Plants, Tong Yu, Yun Bai, Zhuo Liu, Zhiyuan Wang, Qihang Yang, Tong Wu, Shuyan Feng, Yu Zhang, Shaoqin Shen, Qiang Li, Liqiang Gu, Xiaoming Song

Department of Food Science and Technology: Faculty Publications

Heat shock transcription factor (Hsf) plays a critical role in regulating heat resistance. Here, 2950 Hsf family genes were identified from 111 horticultural and representative plants. More Hsf genes were detected in higher plants than in lower plants. Based on all Hsf genes, we constructed a phylogenetic tree, which indicated that Hsf genes of each branch evolved independently after species differentiation. Furthermore, we uncovered the evolutionary trajectories of Hsf genes by motif analysis. There were only six motifs (M1–M6) in lower plants, and then four novel motifs (M7–M10) appeared in higher plants. However, the motifs of some Hsf …

Ultraviolet Light-A: A Novel Agent To Dehydrate Foods, Luis J. Bastarrachea

Funded Research Records

No abstract provided.

John Dupre, John Dupre, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

John Dupre recently retired after 25 years as State Director of Nutrition Programs for the State of Louisiana.

Cytotoxic Activity Of Non-Specific Lipid Transfer Protein (Nsltp1) From Ajwain (Trachyspermum Ammi) Seeds, Saud O. Alshammari, Taibah Aldakhil, Qamar A. Alshammari, David Salehi, Aftab Ahmed

Pharmacy Faculty Articles and Research

Background

Trachyspermum ammi, commonly known as Ajwain, is a member of the Apiaceae family. It is a therapeutic herbal spice with diverse pharmacological properties, used in traditional medicine for various ailments. However, all previous studies were conducted using small molecule extracts, leaving the protein’s bioactivity undiscovered.

Aim

The current study aimed to demonstrate the cytotoxic activity of Ajwain non-specific lipid transfer protein (nsLTP1) in normal breast (MCF10A), breast cancer (MCF-7), and pancreatic cancer (AsPC-1) cell lines. Also, to evaluate its structural stability in human serum as well as at high temperature conditions.

Methods

The cytotoxic activity of Ajwain nsLTP1 was …

Paternal Obesity And Effect Of Fish Oil Supplementation On Offspring Metabolic Health, Akriti Shrestha

Theses - ALL

Obesity and overweight arise due to an abnormal fat accumulation in the body that presents a risk to health. Individuals with body mass index (BMI) of greater than 30 kg/m2 are considered obese. CDC data show that 43% of the adults in the US are obese. The prevalence of obesity as well as its strong association with cardiovascular and metabolic diseases are the serious concerns of public health which calls for the need of appropriate diet/nutrition that can minimize the negative effect of obesity. Literature suggests that 50% of the contribution to obesity can be attributed to genetics. However, studies …

Assessing The Microbiological Profile Of Raw Goat Milk And Understanding Producer Practices And Perceptions, Jacinda Skye Leopard

Theses and Dissertations

Raw milk sales are legal in 30 states, including Mississippi, where regulations are largely management-based and not food-safety related. The research objectives were to analyze the microbial profile of raw goat milk from Mississippi farms and assess food safety practices and perceptions of dairy goat farmers. Deficiencies and opportunities for improvement in raw goat milk safety were identified. Staphylococcus spp. was present in most (70%) milk samples but aligned with drying-off. E. coli, Enterobacteriaceae, and coliforms were observed in 4, 10, and 12 samples, respectively; no Salmonella spp. or Listeria monocytogenes were detected. The survey highlighted farmers' need for …

Improving Nutrition And Food Safety Knowledge Of Small-Scale Fish Processors In Delta State, Nigeria, Grace Adeola Adegoye

Theses and Dissertations

Introduction: Fish is an affordable animal source food that provides nutrition and serves as a source of income for many people especially women in Nigeria. Smoking and sun drying are the processing methods in practice that expose the fish products to possible contaminants which may consequentially negate their nutritive value.

Aim: To improve the knowledge of fish processors on nutrition and safe fish handling.

Methodology: A 3-day participatory training was organized to train 122 fish processors, 95 women, and 25 men. The training was conducted in the three senatorial districts in Delta State, Nigeria. Knowledge was assessed using the pre …

Growing Tea In Mississippi, Qianwen Zhang

Theses and Dissertations

Tea is the most popular beverage worldwide, second only to water. The United States is the third largest importer of tea in the world, importing a total of about 260 million pounds tea annually. With increased demand for locally sourced food products, questions are raised regarding whether domestic production of tea is feasible. Compared to major tea producing countries with centuries of growing history, tea production in the U.S. is limited and requires research support in many aspects of tea production. The objectives of this study were to investigate plant growth, leaf morphology, and seasonal chemical compositions in tea leaves …

Evaluation Of The Major Seed Storage Proteins, The Conglutins, Across Genetically Diverse Narrow-Leafed Lupin Varieties, Arineh Tahmasian, Angéla Juhász, James A. Broadbent, Mitchell G. Nye-Wood, Thao T. Le, Michelle L. Colgrave

Research outputs 2022 to 2026

Lupin seeds have an excellent nutritional profile, including a high proportion of protein and dietary fiber. These qualities make lupin seeds an ideal candidate to help meet the growing global demand for complementary sources of protein. Of consequence to this application, there are nutritional and antinutritional properties assigned to the major lupin seed storage proteins—referred to as α-, β-, δ- and γ-conglutins The variation in the abundance of these protein families can impact the nutritional and bioactive properties of different lupin varieties. Hence, exploring the conglutin protein profiles across a diverse range of lupin varieties will yield knowledge that can …

The Application Of Food-Grade Chitosan And Another Food-Grade Compound In Coatings To Control Tyrophagus Putrescentiae (Schrank, Family: Acaridae) Growth On Dry-Cured Hams And Their Impact On Dry-Cured Ham Quality, Wenjie Shao

Theses and Dissertations

The objective of this research was to evaluate if chitosan and another food-grade compound can be incorporated into food-grade coatings in conjunction with propylene glycol and polysaccharides to control Tyrophagus putrescentiae growth without affecting sensory attributes, water activity, and moisture content of dry-cured hams. Each coating solution was coated on ham cubes (2.5 × 2.5 × 2.5 cm³, n = 5/treatment) or infused in ham nets, and dry-cured ham cubes were wrapped in the ham nets prior to inoculation with 20 mixed-sex adult mites. Randomized complete block designs with two replications were utilized to evaluate the efficacy of …

Biodegradable Active Bio-Nanocomposite Film For The Enhanced Shelf Life Of Tomatoes, Kalpani Y. Perera, Shubham Sharma, Brendan Duffy, Amit K. Jaiswal, Swarna Jaiswal

Articles

The increased environmental pollution has led to finding sustainable solutions for non-renewable plastic-based food packaging materials. Thus, the use of biomaterial-based packaging material has become an immense trend. This work aims at developing an antimicrobial biodegradable chitosan-alginate bio-nano composite film with TiO2 nanoparticle (NPs) for food packaging applications. The film was developed by a solution casting method. The chemical, mechanical, thermal, barrier, antimicrobial, and biodegradable properties of the packaging films were evaluated. Packaging studies were performed for 15 days for cherry tomatoes. The designed packaging material had enhanced the mechanical properties with a significantly (p < 0.05) higher tensile strength of 15.76 folds and 2 fold higher elongation at break. The UV barrier properties increased by 88.6%, while the film transparency decreased by 87.23%. Molecular interaction of N-H covalent bonds was observed between alginate and chitosan together with TiO2 NPs. The developed bio-nano composite film showed antimicrobial activity against foodborne pathogens E. coli, S. aureus, S. typhi, and L. monocytogene with a log reduction of 7.08, 7.28, 6.04 & 6.02 log CFU/ml respectively at 24 hours incubation period. The film was completely biodegraded and a weight loss of 89.06% was observed in bio-nanocomposite film during the 3 months. Shelf-life estimation of cherry tomato using developed packaging films showed an increase in the shelf-life up to 8 days with stable pH, total soluble solids, and weight with no bacterial growth when packaged with prepared film. Owing to their improved mechanical, UV barrier, antibacterial, and biodegradability, the prepared active bio-nano composite packaging films could be considered a potential candidate for fruit packaging.

Market Pressure Based On International Food Standards In Export-Scale Urban Farming: Political Ecology Perspective, Kinanti Indah Safitri, Oekan Soekotjo Abdoellah, Budhi Gunawan, Yusep Suparman, Parikesit Parikesit

The Qualitative Report

Urban farming has been transformed into urban agricultural activities oriented towards optimizing economic benefits through export market involvement. However, the expansion of the market has consequences for farmers. The involvement of urban farmers in export trade causes market pressures that affect agricultural production practices. This research used qualitative research methods. There were 27 informants in this study. Researchers collected data to determine market pressures faced by export-scale urban farmers in Bandung Metropolitan. Data collection techniques used in-depth interviews, observation, and documentation. The results showed that the market had put pressure on export-scale urban farmers in Bandung Metropolitan to meet international …

Comparative Analysis Of Aflatoxin Content In Bitter Almond Fruits (Prunus Dulcis Var. Achchiq) Growing In Various Geographical Region Uzbekistan By High-Performance Liquid Chromatography, Abdukhalil Tojikulov, Khabibjon Kushiyev, Tulkin Djuraev, Hasan Bekpulatov

CHEMISTRY AND CHEMICAL ENGINEERING

The aim of the study was to optimize the process of extraction of aflatoxins secreted by fungi of the genus Aspergillus from almonds, as well as to analyze the content of aflatoxins using high performance liquid chromatography (HPLC). An HPLC LC 2030 C3D Plus (Shimadzu) column Shim-pack GIST-HP C18 150x4.6 mm 3 µm (Shimadzu) was used at a ratio of H₂O/H₃C-CN = 40/60 as an eluent at a thermostat temperature of 40 degrees Celsius. Based on the analysis on a fluorescent detector RF-20A with an excitation wavelength of 365-450 nm and a flow rate of 0.5 …