Food Science Commons^™

Applying Hermetic Storage And Multiple Abiotic-Stressed Germinations To Actuate Desirable Effects In Wheat Phytochemistry, Food, And Biological Functionalities, Bababode Kehinde

Theses and Dissertations--Biosystems and Agricultural Engineering

Wheat is one of the most widely consumed staple foods in the world, supplying both nutritional and caloric requirements for billions of people. The rising global population and increased food demand behooves all stakeholders across the wheat supply chain to significantly increase their output and efficiency. Beyond the target for increased farm yields, dietary, nutritional, and health requirements along with their continuous supply must be satisfied with improved storage and processing schemes. Post-harvest changes in wheat can result in a significant reduction in its quality. Such losses could be qualitative and/or quantitative, impacting its physical, physiological, organoleptic, and nutritional attributes. …

Profiling The Cell Wall Polysaccharides Of Hemp (Cannabis Sativa L.) Inflorescence And Hempseed And Exploring Their Application As Ruminant Feed Ingredients, Miranda Agbana

Theses and Dissertations--Animal and Food Sciences

Hemp (Cannabis sativa L.) is used in the United States as a source of CBD oil, fiber, and grain. While hempseeds are consumed whole or as dehulled hemp hearts, hempseed oil is also a desirable seed oil in human nutrition. The process of collecting hempseed oil leaves behind hempseed cakes that are abundant in protein and fiber. Feeding of hempseed cakes to animals, including ruminants, has only initially been explored. Extraction of CBD from hemp inflorescences generates organic waste that may find a second purpose as a fiber feed for cattle. Little is known about the exact fiber composition …

Plant Cell Wall Composition And In Vitro Fermentation Characteristics Of Cool-Season Forage Grasses From Two Growing Seasons In Central Kentucky, Sophia Danielle Newhuis

Theses and Dissertations--Animal and Food Sciences

Grass cell walls are rich in cellulose, hemicellulosic arabinoxylan (AX) polysaccharides, and lignin. AX structural differences such as degree and pattern of branching and the ester-linked phenolic acid content could affect plants’ digestibility when used as forage for livestock. However, there is little information about how these structural elements change over the growing season in the vegetative tissue of cool-season perennial grasses. Enhanced information about the cell wall composition and carbohydrate structure of forage material will provide a foundation for expanding our knowledge of how forage cell wall carbohydrate structures are utilized by ruminants. The objectives of this study were …

Association Of A Green Tea Extract With Serum Immunoglobulin G Status And Neonatal Vitality In Newborn Dairy Calves, M. E. Reis, Melissa C. Cantor, C. M. M. Bittar, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

Poor vigor at birth has been associated with reduced IgG absorption from colostrum and a reduced vitality in neonatal dairy calves. Some natural compounds, such as green tea extract, may improve vitality in compromised calves. The objective of this randomized controlled trial was to evaluate the potential of supplementing a green tea extract (15 mL) to calves to improve vigor and activity behavior for the first 72 h postnatal. Also, this study aimed to investigate the influence of green tea extract supplementation on calf serum IgG concentration and the apparent efficiency of absorption (AEA) of colostral IgG. Holstein calves (n …

Body Condition Score Change Throughout Lactation Utilizing An Automated Bcs System: A Descriptive Study, Carissa M. Truman, Magnus R. Campler, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

Body condition scoring (BCS) is a traditional visual technique often using a five-point scale to non-invasively assess fat reserves in cattle. However, recent studies have highlighted the potential in automating body condition scoring using imaging technology. Therefore, the objective was to implement a commercially available automated body condition scoring (ABCS) camera system to collect data for developing a predictive equation of body condition dynamics throughout the lactation period. Holstein cows (n = 2343, parity = 2.1 ± 1.1, calving BCS = 3.42 ± 0.24), up to 300 days in milk (DIM), were scored daily using two ABCS cameras mounted …

Form Of Supplemental Selenium Affects The Expression Of Mrna Transcripts Encoding Selenoproteins, And Proteins Regulating Cholesterol Uptake, In The Corpus Luteum Of Grazing Beef Cows, Sarah N. Carr, Benjamin R. Crites, Joy L. Pate, Camilla H. K. Hughes, James C. Matthews, Phillip J. Bridges

Animal and Food Sciences Faculty Publications

Selenium (Se)-deficient soils necessitate supplementation of this mineral to the diet of forage-grazing cattle. Functionally, Se is incorporated into selenoproteins, some of which function as important antioxidants. We have previously shown that the source of supplemental Se; inorganic (sodium selenite or sodium selenate; ISe), organic (selenomethionine or selenocysteine; OSe) or 1:1 mix of ISe and OSe (MIX), provided to Angus-cross cows affects concentrations of progesterone (P4) during the early luteal phase of the estrous cycle. In this study, we sought to investigate (1) the effect of form of Se on the expression of mRNA encoding selenoproteins in the corpus luteum …

The Effect Of Batter Characteristics On Protein-Aided Control Of Fat Absorption In Deep-Fried Breaded Fish Nuggets, Lulu Cui, Jiwang Chen, Yuhuan Wang, Youling L. Xiong

Animal and Food Sciences Faculty Publications

Soy protein (SP), egg white protein (EP), and whey protein (WP) at 6% w/w were individually incorporated into the batter of a wheat starch (WS) and wheat gluten (WG) blend (11:1 w/w ratio). Moisture adsorption isotherms of WS and proteins and the viscosity, rheological behavior, and calorimetric properties of the batters were measured. Batter-breaded fish nuggets (BBFNs) were fried at 170 °C for 40 s followed by 190 °C for 30 s, and pick-up of BBFNs, thermogravimetric properties of crust, and fat absorption were determined. The moisture absorption capacity was the greatest for WS, followed by WG, SP, EP, and …

An Evaluation Of Eragrostis Tef For Future Cultivar Development, Eric Wayne Luteyn

Theses and Dissertations--Plant and Soil Sciences

The many benefits of utilizing teff (Eragrostis tef) as small grain is well documented in journal publications. Over time the domestication of this species has become essential to the diets of the local population and the livestock living in the African highlands. Current research points to a high diversity of trait characteristics found in many teff populations and the potential utilization of these traits to increase desirable characteristics in both grain and forage varieties. With the overarching goal of adding to what is already known about teff, this study evaluates the different traits that are of interest to …

Application Of Mass Spectrometry For Characterization Of Plant-Based Phenolics And Alkaloids, Masoumeh Dorrani

Theses and Dissertations--Chemistry

Plant-derived compounds have the potential to produce value-added compounds with a variety of applications. For example, the lignin part of the lignocellulosic biomass, produced in large quantities as waste from the paper and pulp industries, is a rich source of phenolics with potential applications in the renewable energy sector, pharmaceutical, and chemical industries. On the other hand, plant alkaloids are the primary source for developing plant-derived therapeutics. Unfortunately, the recalcitrant nature of plant cell walls, low extraction yields of small secondary metabolites, and the lack of effective analytical methods for a rapid and accurate identification of plant-based compounds and plant’s …

Application Of A Bacteriophage–Sanitizer Combination In Post-Harvest Control Of E. Coli O157:H7 Contamination On Spinach Leaves In The Presence Or Absence Of A High Organic Load Produce Wash, Badrinath Vengarai Jagannathan, Steven Kitchens, Stuart Price, Melissa Morgan, Paul Priyesh Vijayakumar

Animal and Food Sciences Faculty Publications

Foodborne illness due to the consumption of contaminated products continues to be a serious public health issue. Bacteriophages might provide a natural and effective way to control and reduce the pathogenic bacterial population on food products. Researchers have conducted various experiments to prove their effectiveness against different pathogens and their ability to act as a natural intervention to control pathogen populations, especially in the food industry. In this study, a cocktail of bacteriophages (phages) was added to wash water in the presence of a high organic load along with commercially used sanitizers (chlorine or Sanidate 5.0) to study the efficacy …

Protein Oxidation In Foods: Mechanisms, Consequences, And Antioxidant Solutions, Mario Estévez, Youling L. Xiong

Animal and Food Sciences Faculty Publications

No abstract provided.

Physicochemical And Microstructural Characterization Of Whey Protein Films Formed With Oxidized Ferulic/Tannic Acids, Yaosong Wang, Youling L. Xiong

Animal and Food Sciences Faculty Publications

Protein-based biodegradable packaging films are of environmental significance. The effect of oxidized ferulic acid (OFA)/tannic acid (OTA) on the crosslinking and film-forming properties of whey protein isolate (WPI) was investigated. Both of the oxidized acids induced protein oxidation and promoted WPI crosslinking through the actions of quinone carbonyl and protein sulfhydryl, and amino groups. OTA enhanced the tensile strength (from 4.5 MPa to max 6.7 MPa) and stiffness (from 215 MPa to max 376 MPa) of the WPI film, whereas OFA significantly increased the elongation at break. The water absorption capability and heat resistance of the films were greatly improved …

Partial Removal Of Phenolics Coupled With Alkaline Ph Shift Improves Canola Protein Interfacial Properties And Emulsion In In Vitro Digestibility, Jiang Jiang, Yunqing Nie, Xuemei Sun, Youling L. Xiong

Animal and Food Sciences Faculty Publications

The effect of polyphenol removal (“dephenol”) combined with an alkaline pH shift treatment on the O/W interfacial and emulsifying properties of canola seed protein isolate (CPI) was investigated. Canola seed flour was subjected to solvent extraction to remove phenolic compounds, from which prepared CPI was exposed to a pH₁₂ shift to modify the protein structure. Dephenoled CPI had a light color when compared with an intense dark color for the control CPI. Up to 53% of phenolics were removed from the CPI after the extraction with 70% ethanol. Dephenoled CPI showed a partially unfolded structure and increased surface hydrophobicity …

Nutraceutical Intervention With Colostrum Replacer: Can We Reduce Disease Hazard, Ameliorate Disease Severity, And Improve Performance In Preweaned Dairy Calves?, Melissa C. Cantor, D. L. Renaud, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

The objective of this randomized clinical trial was to determine if an intervention with colostrum replacer (CR; Premolac Plus, Zinpro; 125 g/d fed for 3 d) or a placebo milk replacer (Cows Match, Land O'Lakes Inc.; 125 g/d for 3 d) following a triggered alarm could ameliorate disease bouts in dairy calves. The alarm was set to detect negative deviations of milk intake (20% reduction) or drinking speed (30% reduction) in relation to a calf's 12-d rolling average feeding behavior. Calves were enrolled on this study (n = 42 CR, n = 42 placebo) when they triggered an alarm from …

Efficacy Of Bacteriophage Cocktail To Control E. Coli O157:H7 Contamination On Baby Spinach Leaves In The Presence Or Absence Of Organic Load, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan

Animal and Food Sciences Faculty Publications

Fruits and vegetables are high in nutrients that are essential for a healthy lifestyle. However, they also harbor an extensive array of microorganisms such as bacteria, which can be beneficial, neutral, or pathogenic. Foodborne pathogens can contaminate produce at any stage from the farm to the consumer’s table. Appropriate washing techniques using sanitizers can reduce the risk of pathogen contamination. Issues related to maintaining concentration, efficacy, and other problems have been a challenge for the food industry and, when left unresolved, have led to different outbreaks of foodborne illnesses. In this study, the efficacy of a lytic bacteriophage cocktail was …

Coccidia Vaccine Challenge And Exogenous Enzyme Supplementation In Broiler Chicken 1. Effect On Digesta Viscosity, Diet Energy Utilization, And Apparent Metabolizable Energy Value Of Wheat, Andrew E. Dunaway, Sunday A. Adedokun

Animal and Food Sciences Faculty Publications

This study examined the effect of exogenous mixed-enzyme supplementation (xylanase, β-glucanase, and pectinase) to a corn-SBM (CS) and a wheat-CS-based (WCS) diet in birds challenged with coccidia vaccine (Coccivac B-52™). The WCS-based diet was produced by replacing 30% of the energy-yielding portions of the CS-based diet with wheat. On day 14, 448 (n = 7) Cobb by-product breeder male broilers were assigned to a 2 (diet types) × 2 (with or without enzyme supplementation) × 2 (0 or 20 × coccidia vaccine challenge; CVC) factorial arrangement of treatments in a completely randomized design for the determination of the apparent …

Broiler Genetics Influences Proteome Profiles Of Normal And Woody Breast Muscle, Xue Zhang, K. Virellia To, Tessa R. Jarvis, Yan L. Campbell, Jasmine D. Hendrix, Surendranath Suman, Shuting Li, Daniel S. Antonelo, Wei Zhai, Jing Chen, Haining Zhu, M. Wes Schilling

Animal and Food Sciences Faculty Publications

Wooden or woody breast (WB) is a myopathy of the pectoralis major in fast-growing broilers that influences the quality of breast meat and causes an economic loss in the poultry industry. The objective of this study was to evaluate growth and proteome differences between 5 genetic strains of broilers that yield WB and normal breast (NB) meat. Eight-week-old broilers were evaluated for the WB myopathy and divided into NB and WB groups. Differential expression of proteins was analyzed using 2-dimensional gel electrophoresis and LC-MS/MS to elucidate the mechanism behind the breast myopathy because of the genetic …

Animal And Plant Protein Oxidation: Chemical And Functional Property Significance, Youling L. Xiong, Anqi Guo

Animal and Food Sciences Faculty Publications

Protein oxidation, a phenomenon that was not well recognized previously but now better understood, is a complex chemical process occurring ubiquitously in food systems and can be induced by processing treatments as well. While early research concentrated on muscle protein oxidation, later investigations included plant, milk, and egg proteins. The process of protein oxidation involves both radicals and nonradicals, and amino acid side chain groups are usually the site of initial oxidant attack which generates protein carbonyls, disulfide, dityrosine, and protein radicals. The ensuing alteration of protein conformational structures and formation of protein polymers and aggregates can result in significant …

Procuring Produce In A Rural, Appalachian County: A Thematic Analysis Of Community Member Experiences, Caroline Blincoe

Theses and Dissertations--Nutrition and Food Systems

Rates of obesity and other health disparities are exceptionally high in rural Appalachian counties compared to the nation as a whole. One causal factor of these health disparities in Appalachian counties is the inequitable allocation of healthy food. Food insecurity and the local food environment are large drivers for obesity experienced by Martin County, Kentucky residents. Successful socioecological model (SEM) and policy, systems, and environmental (PSE) interventions have shown promising results in reducing obesity and enhancing food security in this population. Through the transcription of semi-structured focus group interviews, thematic analysis aimed to obtain perspectives on the local food system. …

Physicochemical And Functional Property Modification Of Myofibrillar Protein By Phenolic Compounds Under Oxidative Stress, Anqi Guo

Theses and Dissertations--Animal and Food Sciences

Polyphenol-rich spices and extracts of phenolic compounds are widely utilized in meat processing to modify product flavors. Chemically, polyphenols are reactive with myofibrillar protein (MP), the most functional fraction of all muscle proteins responsible for texture development in comminuted meat products. Such protein–polyphenol interaction is prevalent under oxidative conditions that are common in meat processing. As a large group of phytochemicals with diverse structures, phenolic compounds are known to interact with MP with varying efficacies. Yet, the structure-function relationship of polyphenols in eliciting modification of MP is poorly understood. The overall objective of this dissertation research was to elucidate the …

Arabinoxylan Structural Profiling Of Cool-Season Pasture Grasses Via High-Performance Anion-Exchange Chromatography With Pulsed Amperometric Detection (Hpaec-Pad) Analysis Of Endoxylanase Digests, Glenna Erin Joyce

Theses and Dissertations--Animal and Food Sciences

Arabinoxylan (AX) is a major structural polysaccharide found in the cell walls of monocots such as cereal grains and pasture grasses. The variety of AX structural components and substitution patterns contribute to AX structural diversity between different monocot species as well as plant tissues.

The rumen is the first digestion site of masticated food material in cattle and provides 70% of energy to host through fermentation of forage. There are many species of pasture grasses that act as a forage source. Differences in AX structure found in these pasture grasses may impact rumen microbial fermentation. Understanding the AX structure of …

Regression-Derived Ileal Endogenous Amino Acid Losses In Broiler Chickens And Cannulated Pigs Fed Corn Fiber, Wheat Bran, And Pectin, Sunday A. Adedokun, Olayiwola Adeola

Animal and Food Sciences Faculty Publications

The objective of these studies was to determine the effect corn fiber (CF), wheat bran (WB) and pectin (PEC) on basal ileal endogenous amino acid (EAA) losses in broiler chickens (Exp. 1) and cannulated pigs (Exp. 2) using the regression method. Semi-purified diets containing 100 g/kg of CF, WB, or PEC (broiler chickens) and CF or PEC (pigs) were fed to replicate cages consisting of eight birds per cage of 21-d-old broiler chickens and six replicates of pigs consisting of one pig per pen. Ileal endogenous His, Glu, and Pro losses were higher (p < 0.05) for CF and WB compared with birds fed diets containing PEC. Contrasts between CF and WB showed higher (p < 0.05) ileal endogenous nitrogen, total amino acid, His, Ile, Met, Glu, Pro, and Tyr losses in birds fed the CF diets (Exp. 1). Contrasts of EAA losses between birds fed the WB and PEC diets showed higher (p < 0.05) losses for His, Glu, and Pro. In the cannulated pigs, CF resulted in higher (p < 0.05) ileal endogenous His, Leu, and Tyr losses. In summary, CF induced higher ileal EAA losses in broiler chickens and cannulated pigs.

Effects Of Housing System On Dairy Heifer Replacement Cost From Birth To Calving: Evaluating Costs Of Confinement, Dry-Lot, And Pasture-Based Systems And Their Impact On Total Rearing Investment, Anna Catherine Hawkins, Kenneth H. Burdine, Donna M. Amaral-Phillips, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

Replacement heifer rearing is critical for the future of dairy operations, to improve genetic merit and maintain herd size. A myriad of options exist on how to manage, feed, and ultimately raise replacement heifers. Pasture is perceived to offer optimal welfare and an economical housing system for replacement animals, but confinement systems are gaining popularity. This study investigates the costs associated with replacement heifer management decisions from birth to calving, considering the factors of housing systems, labor, feed, and health. The objective of this study was to develop an economic model to determine the cost of raising a replacement heifer …

Detection Of Organic Free Radicals And Reactive Oxygen Substances Using Chemically-Stimulated Luminescence From Structured Compound Semiconductors, William L. Boatright

Animal and Food Sciences Faculty Patents

A method of monitoring for the presence of an oxidizing chemical species in an aqueous or non-polar environment includes steps of exposing a structured compound semiconductor material to the oxidizing chemical species in the environment and detecting electromagnetic radiation emitted by the structured compound semiconductor material upon exposure to the oxidizing chemical species. The structured compound semiconductor material is a phytate scaffold material and a metal dopant.

Detection Of Hydroperoxides Using Chemically-Stimulated Luminescence From Structured Compound Semiconductors, William L. Boatright

Animal and Food Sciences Faculty Patents

A method of monitoring for the presence of a hydroperoxide in an aqueous or non-polar environment includes steps of exposing a structured compound semiconductor material with peroxidase activity to the hydroperoxide in the environment and detecting electromagnetic radiation emitted by the structured compound semiconductor material with peroxidase activity upon exposure to the hydroperoxide. The luminescence emitting semiconductor material with peroxidase activity consists of a primary semiconductor material with a dopant prepared on a phytate scaffold.

Synthetic Alkaloid Treatment Influences The Intestinal Epithelium And Mesenteric Adipose Transcriptome In Holstein Steers, Kyle J. Mclean, Ransom L. Baldwin Vi, Congjun Li, James L. Klotz, J. Lannett Edwards, Kyle R. Mcleod

Animal and Food Sciences Faculty Publications

Holstein steers (n = 16) were used to determine if a synthetic alkaloid, bromocriptine, would alter the transcriptome of the small intestine and adjacent mesenteric adipose. On d 0, steers were assigned to one of two treatments: control (CON; saline only) or bromocriptine (BROMO; 0.1 mg/kg BW bromocriptine mesylate injected intramuscularly every 3 d for 30 d). Steers were slaughtered and midpoint sections of jejunal epithelium and associated mesenteric fat were collected for RNA isolation. Transcriptome analysis was completed via RNA-Seq to determine if BROMO differed compared with CON within intestinal epithelium or mesenteric adipose mRNA isolates. Differential expression …

Effects Of Supplementation Of Microalgae (Aurantiochytrium Sp.) To Laying Hen Diets On Fatty Acid Content, Health Lipid Indices, Oxidative Stability, And Quality Attributes Of Meat, Bing Liu, Jiang Jiang, Dongyou Yu, Gang Lin, Youling L. Xiong

Animal and Food Sciences Faculty Publications

The present study is conducted to investigate the effects of dietary docosahexaenoic acid (DHA)-rich microalgae (MA, Aurantiochytrium sp.) on health lipid indices, stability, and quality properties of meat from laying hens. A total of 450 healthy 50-wk-old Hy-Line Brown layers were randomly allotted to 5 groups (6 replicates of 15 birds each), which received diets supplemented with 0, 0.5, 1.0, 1.5, and 2.0% MA for 15 weeks. Fatty acid contents and quality properties of breast and thigh muscles from two randomly selected birds per replicate (n = 12) were measured. The oxidative stability of fresh, refrigerated, frozen, and cooked …

Potential For Bacteriophage Cocktail To Complement Commercial Sanitizer Use On Produce Against Escherichia Coli O157:H7, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan

Animal and Food Sciences Faculty Publications

The increasing concern for food safety has created a need to evaluate novel techniques to eliminate or control pathogens, resulting in safe food. In this study, four bacteriophages of bovine origin, specific to E. coli O157:H7, were successfully isolated and characterized. A microplate reader assay demonstrated the efficacy of the bacteriophage (phage) cocktail against E. coli O157:H7 resulting in a significant reduction (p < 0.01) in the target pathogen population. The phage cocktail demonstrated significant efficacy (p < 0.05) against E. coli O157:H7 in the presence of the most utilized sanitizers in the United States, namely 100 parts per million (ppm) free chlorine and 100-ppm peroxyacetic acid. Survival in the sanitizer concentrations demonstrates the potential …

Response Of Broiler Chickens In The Starter And Finisher Phases To 3 Sources Of Microbial Phytase, O. O. Babatunde, J. A. Jendza, P. Ader, P. Xue, Sunday A. Adedokun, O. Adeola

Animal and Food Sciences Faculty Publications

A broiler chicken study was conducted for 42 D to evaluate their responses to 3 commercially available microbial phytases. Growth performance, nutrient digestibility, and bone mineralization at days 21 and 42 posthatching were used as parameters of evaluation. The study was a randomized complete block design with 12 treatments, 8 replicate pens, and 25 birds per pen. Treatments included a positive control (PC), a negative control (NC) with crude protein (CP), nonphytate phosphorus (P), and calcium (Ca) reduced by 18, 1.5, and 1.8 g/kg, respectively; the NC + 4 levels of phytase A (250, 500, 750, 1,000 FTU/kg), 3 levels …

Food Frontiers: An Academically Sponsored New Journal, Li-Shu Wang, Jesus Simal-Gandara, Ming Du, Baiyi Lu, José L. Quiles, Zhen-Yu Chen, Bernhard Hennig, Mingfu Wang, Hang Xiao, Jayashree Arcot, Tianli Yue, Baodong Zheng, Xiaobo Zou, Yoshinori Marunaka, Lianzhong Ai, Weibin Bai, Maurizio Battino, Francesca Giampieri, Milen I. Georgiev, Xiaojun Liao, Youling L. Xiong

Superfund Research Center Faculty Publications

No abstract provided.