Food Science Commons^™

Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh

Faculty Works

The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …

Cytoplasmic Filament-Deficient Mutant Of Treponema Denticola Has Pleiotropic Defects, Jacques Izard, William A. Samsonoff, Ronald J. Limberger

Department of Food Science and Technology: Faculty Publications

In Treponema denticola, a ribbon-like structure of cytoplasmic filaments spans the cytoplasm at all stages of the cell division process. Insertional inactivation was used as a first step to determine the function of the cytoplasmic filaments. A suicide plasmid was constructed that contained part of cfpA and a nonpolar erythromycin resistance cassette (ermF and ermAM) inserted near the beginning of the gene. The plasmid was electroporated into T. denticola, and double-crossover recombinants which had the chromosomal copy of cfpA insertionally inactivated were selected. Immunoblotting and electron microscopy confirmed the lack of cytoplasmic filaments. The mutant was further …

Food Safety In The United States And The European Union: Sequel To A Case Study, Bryan Harris

RISK: Health, Safety & Environment (1990-2002)

The author reports on recent regulatory and legislative developments in the United States and European Union relating to toys imbedded in candy.

Practicality Of Large Mylar Bags To Store Corn And Beans In Kenya, Byu Food Quality Assurance Laboratory

Faculty Publications

The purpose of this study is to determine the practicality of using extra large zip-lock mylar bags with oxygen absorber packets to store corn and beans in Chyulu, Kenya. Weevil usually gets in the food after about 5 months, so if the oxygen can be maintained at a low level, the corn and beans could be stored for longer than that. The goal is to be able to store the food for 18 months. In order to determine the practicality of using the mylar bags to store corn and beans, the following questions will need to be answered: · Are …

Quality Of Shredded Carrots As Affected By Packaging Film And Storage Temperature, Catherine Barry-Ryan, David O'Beirne

Articles

Different packaging films and storage temperatures were used to establish a range of equilibrium modified atmospheres for storage of shredded carrots. Quality and storage-life of the packaged shredded carrots were determined using sensory evaluation, microbial counts, and a range of physical tests. Minimal processing steps such as peeling and shredding caused physical damage, physiological stress, and enhanced microbial growth, leading to a reduced shelf life when compared to the whole vegetable. A P-plus microporous film (CO2 permeability of 29103mL.m 2.d 1.atm 1) was the most suitable for the storage of shredded carrots. Findings indicated that deterioration in these products was …

Commercial Irrigation And Nutrition Practices For Strawberries In Western Australia, D. Phillips, D. Gatter, T. Calder, D. Pitman

Horticulture research reports

Irrigation practices employed by five commercial strawberry growers on sandy soils of the Swan Coastal Plain near Perth Western Australia were monitored for two consecutive seasons, 1995 and 1996 during the Spring production period.

Nutritional practices for the same grower group plus one Albany grower were monitored by plant, soil and sap analysis at 14 day intervals throughout the 1996 season.

Irrigation records showed that market quality strawberries could be produced at this time of year (August to December in Perth) on soils with very poor water retention properties with an average crop factor(E Pan) for the season of 0.7 …

Fermentation Of Fructooligosaccharides By Lactic Acid Bacteria And Bifidobacteria, Handan Kaplan, Robert W. Hutkins

Department of Food Science and Technology: Faculty Publications

Lactic acid bacteria and bifidobacteria were screened of their ability to ferment fructooligosaccharides (FOS) on MRS agar. Of 28 strains of lactic acid bacteria and bifidobacteria examined, 12 of 16 Lactobacillus strains and 7 of 8 Bifidobacterium strains fermented FOS. Only strains that gave a positive reaction by the agar method reached high cell densities in broth containing FOS.

The Effect Of Exopolysaccharide-Producing Cultures On The Moisture Retention And Functional Properties Of Low Fat Mozzarella Cheese, David B. Perry

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Low fat Mozzarella cheese was made using exopolysaccharide-producing starter cultures consisting of single strains of Streptococcus thermophilus MR-1C and Lactobacillus delbrueckii ssp. bulgaricus MR-1R with or without the addition of mesophilic exopolysaccharide-producing adjunct mixed culture consisting of Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. cremoris. A control cheese was made using a non-exopolysaccharide-producing starter culture consisting of S. thermophilus TA061 and Lactobacillus helveticus LH100. Cheeses were analyzed for moisture, melt, fat, and protein. Cheeses made with the addition of the mesophilic exopolysaccharide-producing adjunct culture showed significant differences in moisture, but not in melting properties when compared to cheeses …

Affinity Purification Of Bovine Lactoferrin And Bovine Transferrin From Using Immobilized Gangliosides, Seung-Hee Nam

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bovine lactoferrin (BLF) and bovine transferrin (BTF) are major-iron transport and regulation proteins found in bovine whey. BLF and BTF must interact with the eukaryotic cell surface to mediate their biological function of iron delivery and cellular functions of inflammatory and immunological modulation. As common components of the eukaryotic cell surface, gangliosides were used for affinity purification of BLF and BTF.

Bovine gangliosides were isolated from fresh buttermilk and covalently immobilized onto controlled-pore glass beads (66 μg/g beads). After the matrix was loaded with whey protein (WPI or WPC), lactoferrin was eluted with 1 M NaCl and identified by N-terminal …

Influence Of Limited Proteolysis, Heat Treatment, And Ph On The Whiteness Of Skim Milk, Xiaoshan Li

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Health consciousness drives people to drink skim milk. Yet, improving the blue-white appearance and watery texture of skim milk is necessary to make consumers happy with skim milk. In this study, the influence of limited proteolysis with soluble or immobilized proteases, heat treatment, and pH on the whiteness of skim milk were examined to meet this goal.

Limited proteolysis with milk-clotting enzymes increased the whiteness of skim milk. the proteases porcine pepsin and chymosin were immobilized onto nonporous ceramic, glass, and controlled pore glass (CPG) beads. The enzymes were coupled to beads either directly or via crosslinker proteins. Pepsin, immobilized …

The Effect Of Buttermilk Fraction Concentrates On Growth And Iron Uptake And Transport By Caco-2 Cell Cultures, Yoo-Hyun Lee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To examine the effect of buttermilk fractions on growth, iron transport, and uptake, Caco-2 cells (human colon adenocarcinoma) were grown in a bicameral chamber. The Caco-2 cell culture system is a useful model to study micronutrient utilization in the human enterocyte, because Caco-2 cells continuously differentiate and form a monolayer, which has high polarity, a well-developed brush border, and a tight junction.

Iron bioavailabilty in various milks is very different depending upon milk composition. The fat fraction especially is known to be associated with iron absorption, because the fat fraction has milk fat globule membrane (MFGM), which contains bioactive molecules …

Effects Of Peeling Methods On The Quality Of Ready-To-Use Carrot Slices, Catherine Barry-Ryan, David O'Beirne

Articles

The effects of methods used for peeling and physiological age (over-wintering) on the quality and storage life of carrot disks packaged in modified atmosphere were examined. Commercial mechanical abrasion peeling using fine or coarse carborundum plates, was compared with carrots peeled by hand, before slicing into disks. Slices which had been peeled by abrasion had higher respiration rates, greater microbial contamination and growth rates, higher pH values, higher rates of weight loss and shorter microbiological shelf-lives than those which had been hand peeled. These results reflected the higher quality of the manually peeled carrots. Micrographs of the peeled surfaces confirmed …

Food For Thought On Cognitive Scripts And Genetically Engineered Food, Ibpp Editor

International Bulletin of Political Psychology

This article identifies some of the common thought sequences seemingly harbored by members of the sociopolitical opposition to the production and sale of genetically engineered food.

The Risks And Benefits Of Genetically Modified Crops: A Multidisciplinary Perspective, Garry D. Peterson, Saul Cunningham, Lisa Deutsch, Jon Erickson, Allyson Quinlan, Ernesto Ráez-Luna, Robert Tinch, Max Troell, Peter Woodbury, Scot Zens

Dartmouth Scholarship

Worldwide, the area planted in genetically modified (GM) crops has increased dramatically in recent years. Between 1996 and 1999, it rose from 1.6 X 10⁶ ha to more than 35 X 10⁶ ha (James 1998, May 1999). This rapid increase has provoked an explosion of concern, particularly in Europe, over the health and environmental impacts of these crops. Despite claims of safety and warnings against popular panic, public concern over GM crops has resulted in changes in their marketing, labeling, planting, and trade. These changes have fueled an increasingly heated debate among environmental advocates, critics of industrial agriculture, …

Integronlike Structures In Campylobacter Spp. Of Human And Animal Origin, Brigid Lucey, D. Crowley, P. Moloney, B. Cryan, M. Daly, F. O'Halloran, E. J. Threlfall, S. Fanning

Department of Biological Sciences Publications

Resistance to antimicrobial agents used to treat severe Campylobacter spp. gastroenteritis is increasing worldwide. We assessed the antimicrobial resistance patterns of Campylobacter spp. isolates of human and animal origin. More than half (n = 32) were resistant to sulphonamide, a feature known to be associated with the presence of integrons. Analysis of these integrons will further our understanding of Campylobacter spp. epidemiology.

Western Australia Soil Acidity Research And Development Update 2000 : Time To Lime, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

We have come a long way over the past three or four years in terms of both, the level of understanding and recognition of Soil Acidity as a major land degradation issue for the wheatbelt of Western Australia, and, in our actions to treat the problem.

During 1999 there were three very significant changes relating to acid soils in Western Australia.

• Lime use in WA increased by over 200,000 tonnes from 1998 to 1999 to a record 653,000 tonnes, which was double the amount applied in 1997.
• The Agricultural Lime Industry adopted a voluntary Lime Industry Code of Practice in …

The Intellectual And Technical Property Components Of Pro-Vitamin A Rice (Goldenricetm): A Preliminary Freedom-To-Operate Review, R. David Kryder, Stanley P. Kowalski, Anatole F. Krattiger

Law Faculty Scholarship

Rice is a staple food for millions of people, predominantly in Asia, but lacks essential nutritional components such as Vitamin A. This is very important for over 180 million children and women of child bearing age who suffer from Vitamin A deficiency in Asia alone. For this reason, an improvement was made under an effort led by Profs. Ingo Potrykus and Peter Beyer by inserting several genes into rice to produce an improved product called GoldenRice. Because GoldenRice has the potential to be easily integrated into the farming systems of the world's poorer regions, the advent of GoldenRice promises to …

Micronesia: Steps In The Right Direction, Aydin Nazmi

Food Science and Nutrition

As I watch the hazy orange sun sink into the still blue sea overlooking Chuuk lagoon, I marked the end of another seemingly ordinary day in the Federated States of Micronesia. But this October day was special—it marked the beginning of a promising new era in Chuuk’s fight against HIV and AIDS. For the past few months, several proceedings had set into motion a cascade of events that would ultimately (hopefully) benefit and educate the people of Chuuk state.

The Ins And Outs Of Fluoride In Human Nutrition, Georgia C. Lauritzen, Deloy G. Hendricks, Ann Baker

All Archived Publications

No abstract provided.

The Potato, Utah State University

All Archived Publications

No abstract provided.

Vegetable Canning Methods In The Pressure Cooker, Georgia C. Lauritzen

Archived Food and Health Publications

This publication is estimated to have been produced in 2000 but the exact date is unknown.

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 1 2000, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Compositional Changes In Selected Minimally Processed Vegetables, Emer O'Reilly

Masters

Compositional, physiological and microbiological changes in selected minimally processed vegetables packaged under a modified atmosphere of 2% oxygen and 5% carbon dioxide were monitored over a ten day storage period at 40 C and 80 C. The analysis targeted specific changes in the nutritional, chemical and physiological make up of the vegetables as well as the changes in the microbial levels. In addition the changes in the gas atmospheres within the packs were monitored. It has been widely accepted that modified atmospheres used to package fresh produce can preserve the product quality and thus extend the shelf life of the …

Letter From The Dean, Charles J. Scifres

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Prediction Of Rice Sensory Texture Attributes Using Spectral Stress Strain Analysis And Jack-Knife Model Optimization, Marura Lenjo, Jean-Francois Meullenet

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Sensory texture characteristics of cooked rice were predicted using an extrusion test and a novel multivariate analysis method. Eleven sensory texture characteristics were evaluated via a trained descriptive panel and predicted for force/deformation spectra with partial least squares regression. Only four sensory attributes—adhesion to lips (Rcal = 0.83), cohesion of bolus (Rcal = 0.78), cohesiveness (Rcal = 0.69), and hardness (Rcal = 0.72)—were successfully predicted from instrumental measurements.

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Stock Market Reactions To Unfavorable Product Information: A Case Study Of Comments On Beef Safety Made On An Oprah Winfrey Show, Elpida Ormanidou, Michael Thomsen

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

This study examines the impact of unfavorable media coverage on the stock market prices of major food companies, an issue of increasing importance to the food industry. The study focuses on the 16 April 1996 Oprah Winfrey Show, a popular television program that raised questions about the safety of the U.S. beef supply. The show resulted in considerable controversy, and some cattle feeders blamed the show for a drop in cattle prices. The focus of this study is on the impact of the program at other stages of the food system. We examined the stock returns of two major beef …

Fissure Characterization Of Rice Kernels Using Video Microscopy, Jerry W. Fendley, Terry J. Siebenmorgen, Rustico C. Bautista

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Fissures are fractures of a rice kernel that can be created during the drying and tempering process. They cause tremendous postharvest losses in milling yield. Understanding why and how rice kernels fissure will lead to optimal drying and tempering operations. This information could also provide input to plant breeders for producing rice cultivars that are more resistant to fissuring. Rice kernels were dried using various air conditions in a controlled environment chamber. The kernels were viewed by video microscopy to observe the occurrence of fissures. A videocassette recorder recorded the images for a 24-hour period after the drying process. The …

Abstracts From The Student Presentations Of The Arkansas Chapter Of Gamma Sigma Delta, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.