Biology Commons^™

Phenological Plasticity Provides Limited Resilience To Climate Warming In A Sea Turtle Species With Temperature-Dependent Sex Determination, Blair P. Bentley, Lisa M. Komoroske, Cintia M. Santos, Armando J. B. Santos, Estefany Argueta, Vic Quennessen, Christina M. Coppenrath, Camille Kynoch, Vincent S. Saba, Claudio Bellini, Rafaely N. M. S. Ventura, J. Wilson White, Mariana M. P. B. Fuentes

Biological Sciences: Faculty Publications

Anthropogenic climate change is threatening global biodiversity, with sea turtles particularly vulnerable as offspring sex and developmental success are strongly influenced by incubation temperature. Behavioral plasticity, including the seasonal distribution of reproductive output, may provide short-term mechanisms for mitigating these impacts. Here, we investigated season-wide hatchling sex ratios and emergence success in a small population of green turtles (Chelonia mydas), tracking individual females across their nesting seasons. Sex ratios varied markedly through the nesting season, with later nests producing a greater proportion of male hatchlings. Moreover, sex ratios were relatively consistent among nests laid by individual females. Overall, females producing …

Enzymatic Synthesis Of Salicylate In Yersina Pestis Proceeds Via Intramolecular Hydroxyl Group Transfer., Clarke Miller

Georgia Journal of Science

The salicylate synthase (YbtS) of Yersinia pestis catalyzes the initial step in the biosynthesis of yersiniabactin, an iron-scavenging siderophore and critical virulence factor. While YbtS is proposed to share a  reaction mechanism with other anthranilate synthase homologs, the exact catalytic pathway has remained unresolved.  In this work, stable isotope (¹⁸O) labeling and mass spectrometry are utilized to clarify the catalytic mechanism of YbtS.  The results of the following set of experiments demonstrate that the salicylate hydroxyl group does not originate from solvent water, directly challenging previously proposed models. Instead, the lack of solvent-labeled oxygen incorporation and the absence …

Contributions Of Arginine And Nitrosative Stress To Huntington’S Disease Progression, Ian D. Gaudet

Electronic Theses and Dissertations 2020 - Present

Neuroinflammation is a well-established component of Huntington's disease (HD) pathogenesis, yet the intricate molecular mechanisms that govern this process remain a topic of ongoing research and debate. This dissertation elucidates a novel pathway linking arginine metabolism and nitrosative stress to core HD pathology. We found that the arginine transporter SLC7A2 is selectively upregulated in various HD cellular models and patient samples. Our findings reveal that this upregulation drives an overactive inflammatory response, leading to abnormally high inducible nitric oxide synthase (iNOS) induction and subsequent nitric oxide (NO) production, which causes excessive protein nitrosylation and an increase in mitochondrial fragmentation.

To …

Comparative Assessment Of Developmental Fidelity In Hpsc-Derived Neural Crest Cells, Michael H. Zepeda

Electronic Theses, Projects, and Dissertations

Neural crest cells (NCCs) are a transient, multipotent cell population that contributes to craniofacial structures, peripheral neurons, melanocytes, and smooth muscle. Defects in NCC development underlie a broad range of congenital disorders known as neurocristopathies. Human pluripotent stem cell (hPSC)-derived NCCs provide an evaluable model for studying early human development; however, existing differentiation protocols produce variable outcomes, and no standardized method consistently generates high-fidelity NCC populations. To address this challenge, we compared five published hPSC-to-NCC differentiation protocols using the H9 human embryonic stem cell line. We hypothesized that specific protocols more faithfully recapitulate neural crest development through coordinated regulation of …

From Genes To Streams: Mitochondrial Approaches To Population Monitoring Of The Santa Ana Sucker Catostomus Santaanae, Mariana Antonio

Electronic Theses, Projects, and Dissertations

This study was developed to use aquatic environmental DNA (eDNA) as a tool to detect the presence of the Santa Ana sucker (Catostomus santaanae), a federally threatened fish species, across the Santa Ana River in Southern California. Environmental DNA is any genetic material that an organism sheds into the environment. We can get an idea of what aquatic species are present when sampling water at a given site. We sequenced the complete mitochondrial genome (mitogenome) of the Santa Ana sucker and designed custom DNA primers to improve eDNA detection accuracy. These primers were selected based on high genetic …

Asm And A Culture Of Sharing, Caring, And Fun In Education, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

One doesn't always equate a professional scientific society with sharing, caring, and fun, but in this age of public distrust, public engagement with science and active student learning are ways to regain trust. ASM holds a leadership position in promoting and recognizing the value of education in the future of our profession and is there to support your educational journey. From K12 outreach and public engagement, to professional development, to presenting and publishing scholarship of teaching and learning, ASM has been at the forefront of biology education. Under the leadership of visionary directors such as Amy Chang and now Irene …

Temperature And Salinity Effects On The Physiology Of Bullfrog Tadpoles (Lithobates Catesbeianus) From A Suburban Temperate Wetland, Angela Brown

Theses, Dissertations and Culminating Projects

One of the most robust amphibians is the American Bullfrog, Lithobates catesbeianus, which is a widespread species that is invasive worldwide, and is broadly tolerant of a range of environmental conditions. Despite this tolerance, survival and performance of adult L. catesbeianus can be negatively affected by increasing temperatures and salinity resulting from anthropogenic impacts, such as climate change and road salts. This study focuses on the effects of acute salinity exposure (mimicking road salt influx into a pond) on L. catesbeianus tadpoles at different larval stages. Increased salinity was predicted to negatively affect two physiological performance measures. Critical maximum temperature …

Bees In The Trap: Leveraging Bumble Bee (Hymenoptera: Apidae: Bombus) Bycatch For Ecological And Genetic Insights, Tori L. Strausser

All Graduate Theses and Dissertations, Fall 2023 to Present

Managers rely on insect monitoring traps to detect insect pests that harm crops and protect food and other agricultural production. However, these traps also capture non-target insects, including bumble bees, raising concerns about whether repeated trapping could harm pollinator populations. This thesis explores whether bees caught as non-targets, also called bycatch, in routine pest monitoring traps can be used to better understand bumble bee populations, and whether traps pose risks to their long-term persistence. Using bumble bees collected from pest surveillance traps placed in crop fields across Utah, this research examined seasonal patterns in body size, timing of capture, and …

Advancing Management Strategies For Two Promising Mason Bee Species (Osmia Ribifloris And Osmia Bruneri) For Berry Pollination, Miranda L. Jones

All Graduate Theses and Dissertations, Fall 2023 to Present

Bee management can broadly refer to human manipulation of one or multiple parts of a bee’s lifecycle. Managing honey bees, commonly referred to as beekeeping, is responsible for providing most pollination for global food crops. However, using more than one bee species for crop pollination can have many benefits. Benefits include better crop pollination and insurance in case one bee species underperforms or its population collapses. Some bees within the genus Osmia are already widely managed for crop pollination because their developmental lifecycle allows bee managers to readily deploy pollinating bees in sync with varying crop blooms, and for certain …

Integrative Taxonomic Analysis Of Cryptic Species Diversity In The Spiny Witch-Hazel Gall Aphid, Hamamelistes Spinosus (Hemiptera: Aphididae: Hormaphidini) In Eastern North America, Gavin Andrew Powell Munson

All Graduate Theses and Dissertations, Fall 2023 to Present

Taxonomy is the practice of identifying, classifying and categorizing species, providing structure for the vast diversity of life. Aphids are an ecologically important group of soft-bodied insects, but they can be difficult to identify because of their tiny anatomical size, reduced or simplified morphological features, and morphological variation within a single species across different generations. In this study, the taxonomy of the spiny witch-hazel gall aphid, Hamamelistes spinosus, is revised. The complex multi-generational life cycle of the aphids, which alternates between witch-hazel and birch trees, has led to confusion regarding past descriptions. Scientists in the late 1800s who were …

Attraction Of Nocturnal Scarab Beetles By Unusual Floral Volatiles In A Banksia (Proteaceae) With Functionally Diverse Pollinators, S. K. Wawrzyczek, B. Bohman, S. L. Krauss, I. M. Butler, G. R. Flematti, K. Farnier, S. E. Hoebee, R. A. Davis, R. D. Phillips

Research outputs 2022 to 2026

Pollination by beetles has evolved multiple times in flowering plants but with relatively few plant species adapted specifically to pollination by scarab beetles (Coleoptera: Scarabaeidae). However, some plant species may produce floral scents and offer floral rewards that attract scarabs alongside other pollinator functional groups. In Banksia attenuata (Proteaceae), nocturnal observations led to the discovery of extensive floral visitation by beetles, alongside birds, mammals and diurnal insects. Given the distinctive melon-like floral scent of B. attenuata, we predicted long-distance attraction of beetles by chemical cues. We undertook floral visitor surveys aided by camera traps and video recordings, and quantified insect …

Land Condition Standards For The Western Australian Nullarbor Pastoral Region, Peter-Jon A. Waddell, Wayne J. Fletcher

Natural resources published reports

This report outlines the quantitative land condition standards that define acceptable pastoral land condition for the Western Australian part of the Nullarbor, to meet the regulatory requirements of the Land Administration Act 1997 and the Soil and Land Conservation Act 1945.

The Department of Primary Industries and Regional Development is developing regional-level pastoral land condition standards for Western Australia as a critical component of the Framework for sustainable pastoral management – revised edition (Fletcher 2022), the purpose of which is to address the Office of the Auditor General’s recommendations to improve the management of pastoral lands in WA …

Fibrous Protein Biomaterials: Characterization Of Solitary Bee Silk And Cocoons, And Development Of Recombinant Hagfish And Bee Silk Materials, Oran Wasserman

All Graduate Theses and Dissertations, Fall 2023 to Present

This dissertation explores two complementary aspects of fibrous protein biomaterials: the characterization of understudied naturally occurring solitary bee silk and cocoons; and recombinant expression and biomaterial formation of recombinant hagfish intermediate filaments (rHIF) and a single Osmia lignaria Say (Hymenoptera: Megachilidae) silk protein. Some solitary bee species, which make up about 75% of all bee species, spin silk to construct protective cocoons during development. Despite the importance of silk and cocoons to the solitary bees' life history, their properties remain largely unexplored. This dissertation presents a novel method for isolating and testing individual silk fibers without interfering with the development …

Carbon Cycling, Salinization, And Long-Term Water Quality Trends In Rocky Mountain Streams, Allyson J. Marrs

All Graduate Theses and Dissertations, Fall 2023 to Present

Humans have greatly affected streams and rivers worldwide through urbanization, agriculture, and water use. The purpose of my dissertation is to increase our understanding of various human impacts on rivers. In chapter two, I aim to determine whether there are differences in greenhouse gas (GHG) emissions between a natural river and manmade canals. I did this by measuring the emissions and dissolved concentrations of three of the most prevalent GHGs, carbon dioxide (CO₂), methane (CH₄), and nitrous oxide (N₂O). I found that most of the sites I studied were emitting CO₂ and CH …

Regional Occurrence And Spatiotemporal Habitat Partitioning Of The Culex Genus Mosquitoes In Salt Lake City And Uintah Basin, Utah, Katelyn Graybeal

All Graduate Theses and Dissertations, Fall 2023 to Present

West Nile virus is the most common mosquito-borne disease in North America and can cause serious illness, including inflammation of the brain and nervous system. Because there is currently no widely available vaccine for humans, preventing mosquito bites and monitoring mosquito populations remain the most effective ways to reduce disease risk. Understanding which mosquito species are present, where they occur, and how they respond to environmental change is critical for protecting public health.

This research examined the mosquito species responsible for spreading West Nile virus in Utah through two complementary studies. The first study focused on the Uinta Basin in …

Drought And Diet Breadth: Does Insect Specialization Influence Herbivory When Plants Are Under Stress?, Jakob Palmer

All Graduate Theses and Dissertations, Fall 2023 to Present

Climate change will alter precipitation worldwide, causing more frequent and severe drought events worldwide. Drought affects many ecological processes, including plant-herbivore interactions, which are critical to biodiversity and ecosystem health. A variety of plant characteristics can influence herbivory – such as how hairy, nutritious, or toxic a leaf is – and drought has been shown to alter many of these traits. Unique characteristics of herbivore species are also important: herbivores can either feed on a variety of plants (generalists) or a group of closely related plants (specialists). Specialists can cope with traits of the plants they eat, while generalists are …

Lizards In The Trees: Structural, Functional, And Behavioral Specializations To A High-Risk Environment, Victor D. Munteanu

All Dissertations

Animals must contend with unexpected changes in their habitat and environment to thrive. Arboreal habitats can consist of a wide variety of environmental features, and failure to adequately contend with these features can result in high risk of injury or death upon falling. Despite these strong selective pressures, many vertebrate lineages have successfully invaded arboreal habitats. Using lizard taxa as a model system, I investigated how morphology and behavior have evolved within this specific habitat transition. I pursued these investigations through complementary analyses including: 1) evaluating the effect of stance height and tail prehensility on perturbation recovery, 2) assessing behavior …

The Past, Present, And Future Of Climate Adaptation In A Widespread Lizard Species, Matthew R. Mcternan

All Dissertations

Climate, as a selective force, shapes patterns of biodiversity globally. However, how species adapt to climate remains a central question of biology. The need to understand climate adaptation is paramount, as global climate change threatens a large portion of global diversity. Climate can impose selection on life by either controlling rates of energy intake and expenditure through effects on body temperature and activity, or by exposing organisms to conditions beyond their physiological limits. Studies of climate adaptation have identified a wide-range of putatively adaptive phenotypic patterns, but genomic evidence is increasingly being used to investigate these patterns and better understand …

Effect Of Food Resource, Preservation Method, And Preservation Duration On Larval Size Of Forensically Important Fly Species (Order: Diptera), John Zukowski

Student Theses

Forensic entomology is a vital discipline that can aid in the proceedings of criminal cases. Larval age and development are important aspects to consider when utilizing blow flies (Family: Calliphoridae) for estimates pertaining to the initial time of colonization (TOC), which contributes to postmortem interval (PMI) estimations. Thus, it is important to understand the factors that influence larval size and development, since larval size is commonly used as a proxy for age. Larvae of three species of flies, Lucilia sericata (Meigen), Calliphora vicina (Robineau-Desvoidy), and Phormia regina (Meigen), were reared on five different food resources. Specimens of L. sericata and …

Transcriptional Regulation In The Uterine Luminal Epithelium, Evelyn A. Carrion

Dissertations and Theses (Open Access)

Transcriptional Regulation in the Uterine Luminal Epithelium

Evelyn A. Carrion

Advisor: Richard R. Behringer, PhD

Abstract

The uterus is an organ comprised of multiple tissues that are essential for women’s health and reproduction. The uterus is composed of multiple tissue layers, including the perimetrium, myometrium, and the endometrium. The endometrium is the inner lining of the uterus, and contains the luminal epithelium, glandular epithelium, and stroma. The luminal epithelium is a simple columnar layer of cells that is necessary for embryo implantation. The uterus is derived in part from the embryonic precursor tissue called the Müllerian duct. There are no …

Alfred Russel Wallace Notes 42: How Accurate Are The Transcriptions Presented At The Alfred Russel Wallace Page Website?, Charles H. Smith

Faculty/Staff Personal Papers

A look is taken at the level of accuracy displayed by the transcriptions of Wallace writings offered at the Alfred Russel Wallace Page website, as determined by a ChatGPT analysis.

Seizure Susceptibility And Morphological Analysis Of A Missense Mutation In Hcfc1a Protein, Jose Manuel Fabela

Open Access Theses & Dissertations

Missense mutations in the HCFC1 gene cause methymalonic acidemia with homocystinuria cblX type (MIM#309541). cblX is an X-linked disorder that is associated with multiple phenotypes such as metabolic defects, failure to thrive, intractable epilepsy, facial dysmorphia, movement disorders, and intellectual disability. The mechanisms underlying these phenotypes are not completely understood. Here we used a zebrafish germline mutant allele (hcfc1aco64/co64) to study seizure susceptibility and adult brain structural malformations. Previous studies have established neural progenitor cell defects and hypoactive mechanistic target of rapamycin (mTOR) in this allele. Given the known role of mTOR in seizure phenotypes, we hypothesized that missense mutation …

An Analysis Of Mating Strategies In Rotifers Inhabiting Temporary Habitats, Ariel Bollinger

Open Access Theses & Dissertations

Reproduction is critical for organisms inhabiting ephemeral aquatic habitats where desiccation risk favors rapid and successful mating. Monogonont rotifers rely on sexual reproduction to produce diapausing eggs that withstand harsh conditions. I investigated how mating behaviors vary among populations of five rotifer species based on the hydroperiod of their habitat: Epiphanes brachionus and E. chihuahuaensis were sampled from habitats with short (SH) and long (LH) hydroperiods; Euchlanis dilatata was collected from a short (SH) and a permanent habitat; and Plationus patulus and Sinantherina socialis were collected from a long (LH) and a permanent (Perm) habitat. I hypothesized that SH populations …

Secondary Biology Teachers’ Understandings Of Growth Mindset, Kathryn Chapman

Dissertations

This case study investigates how high school biology teachers understand and apply growth mindset principles in their daily instruction. Using surveys, semi‑structured interviews, and focus groups, the study examines how biology teachers interpret mindset, how they attempt to enact it, and how curriculum demands shape their efforts. Findings show that teachers’ beliefs about intelligence strongly influence their feedback, expectations, and instructional decisions. Teachers described biology as a subject in which struggle is highly visible, emotionally charged, and often tied to students’ prior knowledge, making mindset work both necessary and challenging. Results also indicate that students’ mindsets are shaped by broader …

The Massbank Contributions Of The Mfam Collaboration, Anusha Ahlendorf, Asaph Aharoni, Khabat Vahabi, Tillmann G. Fischer, Pierre Marie Allard, Megan Augustin, Ulschan Bathe, David Broadhurst, Corey Broeckling, Joerg Buescher, Adrian Covaci, Katyeny Manuela Da Silva, Ric C.H. De Vos, Micha Gracianna Devi, Stefanie Döll, Maximilian Frey, Andrej Frolov, Emmanuel Gaquerel, Vasuk Gautam, Esteban Charria-Girón, Alain Goossens, Jeremy Grosjean, Maria Halabalaki, Elias Iturrospe, Kim Kultima, Stephanie Herman, Toni M. Kutchan, Romain Larbat, René Meier, Eleni V. Mikropoulou, Gregory Mouille, Luca Nicolotti, Nir Shahaf, François Perreau, Pierre Pétriacq, Michael Reichelt, Stacey N. Reinke, Rani Robeyns, Alena Soboleva, Otmar Spring, Akshai Parakkal Sreenivasan, Alain Tissier, Jean Chrisologue Totozafy, Hiroshi Tsugawa, Josep Valls-Fonayet, Maria Van De Lavoir, Justin J.J. Van Der Hooft, Fredd Vergara, David Wishart, Ludger A. Wessjohann, Jean-Luc Wolfender, Jörg Ziegler, Gerd Ulrich Balcke, Steffen Neumann

Research outputs 2022 to 2026

Introduction: The analysis of metabolic profiles using high resolution mass spectrometry (MS) data provides deep insights into biological processes. In metabolomics, MS analysis generates a large number of features that represent metabolites. However, identifying specific metabolites from these features can be challenging. One of the major bottlenecks in the metabolomics field is the identification of MS features, which is a prerequisite for any biochemical interpretation. By identifying similarities and differences within a metabolite family (mFam), evaluating MS features at the metabolite family level can help assigning functional roles to individual MS features. These data can help interpreting metabolic pathways and …

Long-Term Spatiotemporal Change In Coral Reef Fish Populations And Communities In The Philippines, John Christopher Whalen

Biological Sciences Theses & Dissertations

The Visayas Region of the Philippines is a global epicenter of marine fish biodiversity. Hypotheses attribute this peak of species richness within the Coral Triangle to processes that occur at geological time scales. However, changes in biodiversity may occur on ecological time scales, allowing for the assessment of the potential impact of anthropogenic activities. Recent declines in genetic, species, and ecosystem diversity have been observed in the tropics, including the Philippines. Within the Philippines the Visayas Region is considered the epicenter of species adversity due to anthropogenic stressors such as habitat degradation, overfishing, and harvesting individuals for the aquarium trade. …

Modulation Of Electrophysiology And Plaque Aggregation In Huntington’S Disease Patient-Derived Cells By Picosecond Pulsed Electric Fields, Mackenzie A. Tardif-Kunk

Biomedical Sciences Theses & Dissertations

Huntington’s Disease (HD) is a fatal, autosomal dominant neurodegenerative disorder characterized by progressive motor and cognitive decline. Unstable expansion of cytosine-adenine-guanine (CAG) trinucleotide repeats in the huntingtin gene, often exceeding 35–40 repeats, results in an extended polyglutamine (PolyQ) tract in the translated protein. Abnormal folding of the mutant huntingtin (mHTT) protein leads to accumulation and aggregation within the cell, dysregulating various cellular processes through molecular sequestration. While several therapeutic strategies have been proposed, this disease remains incurable with treatments limited to symptom management.

Pulsed electric fields (PEF), primarily in the microsecond and nanosecond ranges, have been studied extensively for the …

The Feeding Ecology, Habitat Use, And Monitoring Of Juvenile Alosines: Alewife (Alosa Pseudoharengus), Blueback Herring (A. Aestivalis), And American Shad (A. Sapidissima) In The Kennebec River, Maine, Brian Determan

All Student Scholarship

The Kennebec River, Maine is home to three species of anadromous fish of the genus Alosa: alewife (A. pseudoharengus) and blueback herring (A. aestivalis), jointly referred to and managed as river herring, as well as American shad (A. sapidissima). These ecologically, economically, and culturally important fish populations are depressed compared to historic levels and have been subject to extensive restoration efforts. While spawning adults have been well-studied, much less is known about the juveniles including whether they engage in resource partitioning to minimize competition and facilitate coexistence. In Chapter 1, I use stomach contents to compare diets as well as …

Extinction Risk Of Coral Reef Fishes In The Philippine Marine Ornamental Trade, Jemelyn Grace Baldisimo Sepulveda

Biological Sciences Theses & Dissertations

Coral reef ecosystems in the Philippines face severe threats from intense fishing and habitat degradation. This dissertation evaluated the extinction risk of fishes in the Philippine Marine Aquarium Trade (MAT) using Productivity Susceptibility Analysis (PSA) and temporal genomics. A global PSA framework was modified by including local susceptibility factors. Of the 103 species evaluated, six were categorized as highly vulnerable and 62 as moderately vulnerable, revealing increased local vulnerability and underscoring the importance of local assessments to guide conservation efforts.

Temporal genomics compared historical (1908–1909 U.S.S. Albatross Expedition) and contemporary populations of the MAT-targeted Sphaeramia nematoptera and Pseudanthias squamipinnis, …

Evaluating Pfas And Marine Microbial Interactions In Estuarine Sediments With Varying Urbanization And Assessments Of Microbial Responses To Pfos, Zachary J. Padgett

All Theses

Per- and polyfluoroalkyl substances (PFAS) are a diverse class of synthetic chemicals that have become globally distributed due to their extensive use in industrial and consumer products and their extraordinary resistance to environmental degradation. Their persistence and documented toxicity have made them a growing concern in coastal environments, particularly in estuaries where urban runoff, wastewater, and industrial discharges concentrate these compounds. Estuaries are unique in that tidal mixing and interactions with fine-grained sediments and organic matter promote PFAS retention, while the microbial communities living directly within estuarine sediments are positioned where PFAS preferentially accumulate, creating conditions for prolonged exposure and …