Biology Commons^™

Isolation And Characterization Of Autosomal Male Sterile Mutants In Drosophila Melanogaster, Lynn J. Romrell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In order to study the genetic control of spermiogenesis, recessive, male-sterile, autosomal mutants of Drosophila melanogaster were induced with ethyl methanesulfonate. A total of 31 mutants were recovered, 15 of which were located on the second chromosome and 16 on the third chromosome. Eight second- and 6 third-chromosome mutants demonstrating sterility in all homozygous males were used for further analysis.

Complementation tests showed that 2 of the 8 second chromosome mutants (and none of the 6 third chromosome mutants) were noncomplementing, indicating that two of the mutants produced were alleles of the same locus. Mapping of the second chromosome mutants …

Investigation Of Urolithiasis Occurring In Hatchery-Reared Trout In Utah, Ernest H. Dean Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The urolithiasis condition investigated occurred at a "hard water" hatchery in Utah after the trout were changed from a meat diet to a commercial pelleted fish feed as a sole sustaining diet in 1961. Numerous diet modifications proved unsuccessful in preventing urolithiasis at the hatchery. It was eventually determined that different commercial diets resulted in varied percentages of trout developing urolithiasis. The urolithic deposits found in the kidney tubules were amorphous and composed of calcium phosphate (apatite) and an organic matrix. Bacterial contamination of the kidneys was not an important factor in the formation of urolithiasis. Rainbow trout containing urolithic …

An Electrophysiological Study Of The Oral Plate Sensory Organs Of The Honey Bee (Apis Mellifera L.), Rodney R. Seeley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The oral plate sense organs of the honey bee, Apis mellifera L., have been investigated employing an electrophysiological technique which allows simultaneous stimulation and recording.

The results of the study present evidence that the four bipolar sense cells innervating the sensory papillae on the oral surface of the hypopharynx are chemoreceptors. The sensory papillae respond to cations, glucose, water, and amino acids. Evidence for the absence of a mechanosensory cell is presented. The data concerning the sensory structures agree with the current theories of chemoreception.

Effects Of Dmso And Propham On Mitosis In Root Tips Of Secale Cereale, Max Rosequist

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

DMSO (dimethyl sulfoxide) and propham (isopropyl carbanilate) were investigated to determine their effect singly and in combination on mitoses in rye (Secale cereale) root tip cells. Root tips were prepared for observation by the paraffin and aceto-carmine squash methods. Results showed DMSO caused the following abnormalities: arrested metaphases, multipolar spindles, multinucleate cells, stickiness, shortened chromosomes, and mitotic inhibition. Cells from propham treated root tips exhibited the same abnormalities as those treated with DMSO. In addition, propham treatments resulted in polyploid cells and chromosome granulation. In combination, DMSO and propham caused all the abnormalities mentioned above except polyploidy. In …

Ribose 5'-Phosphate Aminotransferase Of Salmonella Typhimurium, Gulamnabi Y. Vahora

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ribose-5 '-phosphate aminotransferase, an alternate first enzyme in the purine de novo pathway, has been purified about 800-fold from Salmonella typhimurium (pur D-55 ). The enzyme is distinct from ribosylpyrophosphate-5'-phosphate amidotransferase (E C 2.4.2.14), has an approximate molecular weight of 229,000, and requires ribose-5'-phosphate, ATP and either ammonium ion, carbamyl phosphate or L-glutamine, to synthesize 5'-phosphoribosylamine (PRA). A coupled assay system, employing a S. typhimurium (pur G-310) extract as the coupling-agent source, was used to measure the enzyme. PRA production in this system was inhibited by higher ribose-5' -phosphate concentrations and longer (over 10 minutes) incubation periods. It …

The Suitability Of Newton Reservoir To Be Either A Coldwater Or A Warmwater Fishery, Jeffrey H. Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Measurements were made of the water chemistry the distribution and relative abundance of net-plankton, and the reproduction, age and growth, and food habits of largemouth bass in Newton Reservoir, Newton, Utah.

Samples for chemical analysis were collected weekly from three sites and from several depths at each site. Plankton collections were made on a monthly basis. Data were compared with data in the literature and analyzed for possible ecological implications.

Physical and chemical investigations of the water indicated favorable conditions for a warmwater fishery.

The study of the surface net-plankton revealed a predominance of blue-green algae.

The substratum of the …

A Comparison Of The In Vitro Viability Of Ova From Hormone-Treated Mice With Ova From Spontaneous Ovulations, Henry H. Lyon

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Science and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Henry H. Lyon on April 23, 1971.

A Conceptual Draft Of A Dynamic Hydro-Biological Model For Lake Mead, L. G. Everett, Bureau Of Reclamation

Publications (WR)

Food and energy transformation in an aquatic system must be understood in order to develop a simulation approach. This report provides a comprehensive study of an aquatic ecosystem. The objectives of this report are to show: (1) the biological relationships in an aquatic system (2) the role of nutrients in the biological cycle (3) the role of abiotic factors in a limnetic environment (4) the status of the art of "Eutrophication modeling".

A Determination By Thin-Layer Chromatography Of The Chemical Nature Of The Poison Produced By The Bryozoan Lophopodella Carteri (Hyatt), John Michael Dolan

Master's Theses

A study was undertaken to isolate and characterize by thin-layer chromatography the poisonous principle produced by the freshwater bryozoan, Lophopodella carteri (Hyatt).

The bryozoan homogenate was divided into a lipid and non-lipid portion and each was assayed for toxicity. Only the lipid extract was lethal to fishes. The lipid part was divided into six classes by thin-layer chromatography. When tested, four of the six classes were toxic. Thin­ layer chroatography was used to subdivide each class into fractions. Assays showed toxicity in nine of fourteen fractions.

Evidence indicates that the poisonous princi ple is a lipid and the large number …

Cause And Control Of A Common Market Disease Of Lettuce, William Palmore

Masters Theses & Specialist Projects

Head lettuce, Lactuta sativa var. capitata, is susceptible to a number of economically important diseases, the most frequent being russet spot, rib discoloration, and vascular browning (28), which have been shown by Ceponis and Friedman (7) to be caused by Pseudomonas marginalis. Pseudomonads are common plant pathogens and cause such diseases as halo blight in beans (20), bacterial blight in soybeans (20), and bacterial wilt of the bird-of-paradise (27) and tobacco (24).

Plants that develop symptoms similar to russet of lettuce are oats, infected by Pseudomonas cichorii, and tobacco infected with Pseudomonas tobaci (34). In general, russet symptoms …

Immunologic Enhancement Of Murine Sarcoma I Tumor System, Fung-Win Shen

Biology ETDs

The purpose of this project was to study the mechanism of imnunologic enhancement in the mouse a t the cellular level. The Sarcoma I (Sal, H-2^a) murine tumor system was employed as the model for this study. Sal, indigenous to the A/J (H-2^a) strain of mouse, grows progressively in its syngeneic host but is rejected by non-H-2a mice. However, the SaI tumor can be induced to grow progressively in C57BL/KsJ (Ks, H-2d) mice by passive administration of Ks anti-Sal serum at the time of Sal tumor implantation. This phenomenon is termed immunologic enhancement. Hereafter, mice pretreated …

Molecular Weight Distribution Of Proteins Synthesized In Single, Identified Neurons Of Aplysia, David L. Wilson

Biology Articles and Papers

Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-³H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, …

A History And Distributional List Of Ellis County, Kansas, Birds, Charles A. Ely

Fort Hays Studies Series

This paper marks a century of Ornithology in Ellis County most of it centered around Hays and Ellis. This paper is an attempt to collect the scattered observations of numerous birders and to encourage others to record their observations for posterity.

A Study Of The Endogenous Electron Donors For Nitrogen Fixation By Cell-Free Extracts Of Klebsiella Pneumoniae M5al (Aerobacter Aerogenes M5al), Elisabeth Yi-Jan Wu

Electronic Theses and Dissertations

Biological nitrogen fixation, the mechanism of the enzymatic conversion of the inert nitrogen gas into amino acids in the cytoplasm of the microorganism has puzzled many scientists for decades. The recent discovery of some free-living nitrogen-fixing bacteria has encouraged the investigation of this mechanism. A big step taken in the rapid elucidation of this mechanism was the first reproducible cell-free nitrogen-fixing system from Clostridium pasteurianum successfully obtained by Carnahan et al. (9) in 1960. In the following decade, reproducible cell-free systems were demonstrated with the strict aerobe, Azotobacter vinelandii (4), facultative anaerobic Bacillus polymyxa (16) and Klebsiella pneumoniae (31) and …

Micronutrients And Biological Patterns In Lake Mead, Hasan K. Qashu, Lorne G. Everett, J. S. Carlson, Bureau Of Reclamation

Publications (WR)

Progressive increases in concentration of dissolved solids in the Colorado River water from Lake Powell to Imperial Dam seem to alter plankton dynamics and biological productivity of the river. Also, changes in biological productivity and micronutrients concentrations occur within the same reservoir. Development of a digital simulation model to predict micronutrients concentrations and biological productivity is necessary for diagnosing changes in plankton population and effluent-carrying capacity of the system. The objectives of the study are: (1) to determine trace metal balance at different locations in Lake Mead, (2) to measure biological productivity and conduct plankton population counts at each sampling …

The Effect Of Las Vegas Wash Effluent Upon The Water Quality In Lake Mead, D. A. Hoffman, P. R. Tramutt, F. C. Heller, Bureau Of Reclamation

Publications (WR)

This study developed from observations made during an earlier study on Lake Mead which was reported in CHE-70, Water Quality Study of Lake Mead. Results from that study indicated that poor-quality water was flowing into the Las Vegas Bay reach of Boulder Basin, Lake Mead. Also reports of deteriorating water quality, resulting in taste and odors in domestic water supplies taken from Boulder Basin as well as a reduction in the attractiveness of Las Vegas Bay for recreational uses caused by aquatic plants and algae blooms, indicated a need for a concentrated study concerning the effects of flows from Las …

Combined Microspectrophotometry And Autoradiography Of Late Interphase Nuclei In Physarum Polycephalum, Joseph K. Vlchek

Master's Theses

No abstract provided.

Seasonal Changes In Morphology Of The Gonads Of The Bluegill Lepomis Macrochirus, Alan C. Roman

Master's Theses

No abstract provided.

Notes On The Algae Of Arkansas. 1. Chrysococcus, Kephyrion, Kephyriopsis, Pseudokephyrion And Stenokalyx, Richard L. Meyer

Journal of the Arkansas Academy of Science

The genera Chrysococcus, Kephyrion, Pseudokephyrion and Stonokalyx have received some consideration by investigators in Europe and in the boreal forest and tundra-range regions of the United States. Many of the species are considered to be cold-water stenotherms, thus occurring more frequently in northern habitats. In the present paper 4 species of Chrysococcus, 4 species of Kephyroin, 2 species of Pseudokephyrion and 2 species of Stenokalyx from Arkansas are discussed.

A Variation Of The Standard Steepest Ascent Search Procedure For Response Surface Experiments, George Whitfield Cobb

Theses and Dissertations

The results of this chapter lead to a modification of the standard stopping rule which breathes new life into the method of steepest ascent, but which is unfortunately not sufficient to effect a total resuscitation. As expected, there is no uniformly optimum stopping rule, nor even a uniformly best test statistic; both the optimum statistic and the optimum test level depend on the model parameters y1 and y2. One can, however, adopt a compromise rule which works reasonably well for all parameter combinations by testing at the 25% level with the statistic zn = 1/2(yn -yn-2). In many cases the …

The In Vitro Synthesis Of Melanin In The Avian Pineal When Treated With D, L-Dopa, Bernadette Ann Nowicki

Master's Theses

No abstract provided.

Taxonomic Studies On The Genus Podocotyle (Trematoda: Opecoelidae), Paul Clinton Edmiston

University of the Pacific Theses and Dissertations

The original intent of this study was to reinvestigate and confirm the validity of thirteen species of Podocotyle reported from Dillon Beach, California. Eight species were described by Park (1937) and five described by Johnson (1949-unpublished). Among these species basic similarities were evident in such features as: cirrus sac, seminal vesicle, body shape, extent and distribution of vitellaria, which indicated the possibility of conspecificity. Because four of these species (Podocotly blennicottusi, P. california, P. pedunculata, and P. pacifica) were also reported from fishes of the Pacific Northwest coast by Ching (1960), Pratt and McCauley (1961), Arai (1969), and Hall and …

Lichens Of The Genus Parmelia From Columbia, Costa Rica, And Guatemala, Donald G. Flenniken

Dissertations and Theses @ UNI

During the summer of 1969 a total of eighty-four species of lichens belonging to four subgenera were collected in Colombia, Costa Rica, and Guatemala. Twelve of these are described as new. The subgenus Amphigymnia is represented by thirty-six species, three of which are described as new. The subgenus Parmelia is represented by forty-one species, eight of which are described as new. The subgenera Everniiformes and Xanthoparmelia are represented by two and five species, respectively, with no new taxa recognized. The lichen product strepsiline, previously known only in the lichen Cladonia strepsilis (Ach.) Vain., was found by microchemical tests in Parmelia …

Radio-Biotelemetry Studies Of Circadian Body Temperature Rhythms And Activity Levels [Of The] Zebra Finch (Peophilia Guttata), Vaughan Arthur Langman

University of the Pacific Theses and Dissertations

The object of this study was to determine the normal thermoregulatory patterns of zebra finches (Poephilia guttat) in as near a normal physiological and behavioral state as possible. Surface body temperature and level of activity were the two primary channels of information. Radiobioelemetry allowed the reduction of external disturbance during long periods of continuous recording.

Temperature Induced Changes In The Territorial Dynamics Of Cyprinodon Macularius Baird And Girard, Don Victor Plantz Jr.

University of the Pacific Theses and Dissertations

Behavior is qualitatively and quantitatively modified by the environment. Thus, any consideration of behavior must take into consideration the environmental conditions present. The present study evaluates the interrelationship between one environmental condition (temperature) and a measurable behavioral phenomenon (territoriality).

The Effects Of Para-Chlorophenylalanine On A Conditioned Emotional Response In Rats, Roy J. Hartmann

Theses & Dissertations

The initial intent of this study was to investigate p-CPA's actions on conditioned "anxiety" (CER) in rats in order to ascertain whether the derived effects were qualitatively similar to those previously reported for "conflict" behavior. Specifically, the goals of this study were to determine if p-CPA would reinstate lever-pressing behavior that had been suppressed by a series of tone-shock pairings. If a change in behavior did occur, would the reintroduction of serotonin, through the use of 5-HTP, return the behavior to pre-drug control levels.

Population Variation In Fruit Material Of Acer Negundo L., Robert Williams Jr.

Masters Theses & Specialist Projects

1. Comparisons of the mean weight per hundred fruits of fourteen populations demonstrate a definite cline, seed originating from the northern habitats being heavier.

2. The fruit length, wing length and total length of the fruit material from thirteen populations increases with increase in latitude.

3. The average caloric value for all populations tested was 5,092 +/- 165 calories per gram. Due to the heavier weight of the fruit from the northern provinces, an higher energy content per fruit is indicated with increase in latitude.

4. Stratification and germination tests separate the populations into two large geographical groups with the …

The Application And Refinement Of Thin Layer Chromatographic Procedures In Analyzing The Ribonucleic Acids Present During The Morphological Development Of Two Varieties Of Avena Sativa L., Dorothy Anne Hackett

Masters Theses

No abstract provided.

An Evaluation Of Student Attitudes In The Audio-Tutorial Biological Science Program At Western Michigan University And How They Are Reflected In Student Achievement Versus Time Spent In Laboratory, Dorothy Anne Hackett

Masters Theses

No abstract provided.

Nesting And Brooding Characteristics Of Sharp-Tailed Grouse (Pedioecetes Phasianellus Jamesi Lincoln) In Southwestern North Dakota, Carter Drew Christenson

Theses and Dissertations

A study of the nesting and brood habitat preferences of sharp-tailed grouse (Pedioecetes phasianellus jamesi Lincoln) was conducted in southwestern North Dakota during the reproductive seasons of 1969 and 1970. Radio telemetry was used to monitor the spring and summer activities of seventeen sharp-tail hens. Eight brooding hens were semi-continuously radio-tracked for movement and cover selection. A quantitative and qualitative analysis of seventeen nests and eight renests was made to determine the preferred nesting cover and the effects of intensive grazing upon it .. Nests were established either in non-use areas or in areas of light to moderate grazing. These …