Biology Commons^™

Anything But Another Essay: Understanding Undergraduate Stem Student Perceptions Of Creative, Project-Based Learning, Emily M. Redding, Lorelei E. Patrick

SACAD: Scholarly Activities

The training of innovative and diverse scientists starts with creative teaching practices. Much of the pedagogy literature on incorporating nontraditional teaching practices in college STEM courses focuses on the use of in-class active learning activities. Less study has been devoted to creative, project-based assignments, such as “UnEssays”. The “UnEssay” is a semester-long assignment that challenges students to complete a library research project on a topic of their choosing and present it using anything but an essay. Although gaining popularity, few studies have assessed STEM student's perceptions of the assignments. To do so, Zoology students were asked to complete pre- and …

Screening Environmental Soil Samples For Antibiotic Production, Paige Mattick

SACAD: Scholarly Activities

Antibiotic resistance is a detrimental worldwide challenge, producing bacterial infections that are progressively more difficult to treat and cure. To attempt to help alleviate this issue, we screened soil samples for the presence of antibiotic-producing microorganisms. Soil samples were collected and diluted to 1:100 and 1:1000 ratios of soil and distilled water. These soil mixtures were then streaked onto tryptic soy agar (TSA) plates and incubated at 30℃ until colonies developed. These colonies were then selected and plated on a lawn of Serratia marcescens, which was utilized as the target organism.

Serratia marcescens was selected due to its known resistance …

Presence Of Porcine Endogenous Retrovirus C In Domestic Pigs In Kansas, Avery Welton, Eric T. Gillock

SACAD: Scholarly Activities

There is currently a shortage of organs available to perform allotransplants in humans, prompting the exploration of xenotransplantation as an alternative. Xenotransplantation is the transplantation of living tissues between different species, with porcine tissues being a promising option. However, concerns arise regarding cross-species transmission, particularly in relation to Porcine Endogenous Retroviruses (PERVs). PERVs are retroviruses that are integrated into the germ-line cells of pigs and are permanently embedded in the host cell’s DNA, allowing for vertical transmission. Of significant concern is the infectious nature of two PERV subtypes, A and B, to humans, which can potentially recombine with the third …

Research Proposal: Examination Of Bacterial Communities In Soil Beneath Decaying Organic Matter And The Effect Of Insect Succession, Olivia Fotiadis

2024 Student Academic Showcase

Bacterial species of the Proteobacteria, Acidobacteria, and Bacteroidetes phyla are key components in decomposing organic matter by secreting specialized enzymes into decaying material, facilitating its breakdown and nutrient release. Diverse microbial communities in areas of decomposition are known for their efficiency in decomposition and nutrient cycling. Less is understood about the effect of insects introducing unique microbes alongside initial soil bacteria, like Bacillus subtilis, in the decomposition process. Studying the decomposition of pork samples as a human analog will explore variations in bacterial populations in soil before and after the introduction of insect-mediated microbes and provide information on the efficiency …

The Changes In Oral Flora After Oral Hygiene, Brooklyn Bumbales

2024 Student Academic Showcase

The goal of this project is to study the changes in the oral flora of an individual's mouth pre-cleaning and post-cleaning due to everyday brushing. The oral flora comprises many microorganisms such as Staphylococcus and Lactobacillus that are constantly changing. In previous studies, the diversity and abundance of these microorganisms in the oral cavity are subject to change in the times following pre-cleaning and post-cleaning. This study will examine saliva samples from consenting individuals, will be collected pre-cleaning (brushing) and post-cleaning of the oral cavity. The samples will then be tested for the population size and diversity of different microorganisms …

The Effects Of Ocean Acidification On Sea Urchin Larval Survivorship And Development In Lytechinus Variegatus And Arbacia Punctulata, Emily Pace, Kacie Miller*, Nancy Dalman, Margaret (Meg) Smith

Georgia Journal of Science

Accumulated carbon dioxide in the atmosphere is one of the driving factors in ocean acidification as oceanic absorption of carbon dioxide alters ocean chemistry. Lower concentrations of carbonate ions and higher concentrations of hydrogen ions in the water adversely affect marine organisms, including sea urchin larvae, that use calcium carbonate in their skeletal structures. While there is a wide body of literature demonstrating an impact of lowered pH on sea urchin larval development and survival, it is unclear if the method of pH manipulation and the species being studied influences the results. To address this, we compared two commonly employed …

Examination Of Greenhouse Conditions That Influence Scale Infestation Rates On Begonia, Madeline M. Cusick

Honors College Theses

Scale insects are common pests within greenhouse and agricultural settings. I quantified scale infestation on Begonia x Richmondensis, also known as the Dragon Wing begonia, within the Armstrong Campus Greenhouse from March to November of 2023. Six different treatments accounted for variations in sunlight, humidity, and temperature within the greenhouse with the goal of determining the insect’s preferred environmental conditions. Morphological comparisons were also made between host plant Dragon Wing begonia and other begonias present within the greenhouse. Analysis indicated a significant difference in scale presence on replicate plants by zone and sunlight treatments, with more plants hosting at least …

Continuous Mowing Differentially Affects Floral Defenses In The Noxious And Invasive Weed Solanum Elaeagnifolium In Its Native Range, Alejandro Vasquez, Alexa Alaniz, Robert K. Dearth, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

In weeds, disturbance has been found to affect life history traits and mediate trophic interactions. In urban landscapes, mowing is an important disturbance, and we previously showed that continuous mowing leads to enhanced fitness and defense traits in Solanum elaeagnifolium, Silverleaf Nightshade (SLN). However, most studies have been focused on foliar defenses, ignoring floral defenses. In this study we examined whether continuous mowing affected floral defenses in SLN using mowed and unmowed populations in South Texas, their native range. We found flowers of mowed SLN plants larger but lighter than unmowed plants. Additionally, flowers on plants that were mowed …

Investigating The Role Of Circular Rnas In Metastatic Colorectal Cancer Progression, Sidi Zhao

Arts & Sciences Graduate Student Theses and Dissertations

Colorectal cancer (CRC) is the most common gastrointestinal malignancy and a leading cause of cancer deaths in the U.S.A. More than half of CRC patients develop metastatic disease (mCRC) with a 13% average 5-year survival rate. Despite advances in our understanding of primary CRC oncogenesis and biology, the mechanisms of tumor metastasis remain poorly characterized. To date, CRC research has primarily focused on the dysregulation of protein-coding genes to identify oncogenes and tumor suppressors as potential diagnostic and therapeutic targets, thereby under-representing the critical role of many classes of non-coding RNAs (ncRNAs). One emerging class of ncRNAs, circular RNAs (circRNAs), …

Teaching Evolutionary Principles With Ai Image Generators, Jacqueline Garnett

Generative AI Teaching Activities

The purpose of this assignment is for students to practice constructing phylogenies using the principle of parsimony. In this activity, students will use an AI Image Generator to create a grid of images of the same organism and identify a series of traits that vary to build a possible phylogenetic tree.

Dihydroceramide Desaturase Governs Endoplasmic Reticulum And Lipid Droplet Homeostasis To Promote Glial Function In The Nervous System, Yuqing Zhu

Arts & Sciences Graduate Student Theses and Dissertations

Dihydroceramide desaturase converts dihydroceramides to ceramides in the final step of de novo ceramide biosynthesis. Reduction of dihydroceramide desaturase DEGS1 function in humans leads to hypomyelinating leukodystrophy-18, but the etiology of this disease remains unclear. Using mutants from a forward genetic screen, we discovered that infertile crescent (ifc), the sole Drosophila dihydroceramide desaturase, governs central nervous system development and morphology. ifc is primarily expressed in glia and acts cell-autonomously in glia to control glial morphology and function during nervous system development. Loss of ifc leads to severe glial defects, particularly in cortex glia, including endoplasmic reticulum expansion, glial swelling, compromised …

Interleukin-1Α And Leukemia Inhibitory Factor Promote Cancer-Induced Extramedullary Hematopoiesis, Derek Arthur George Barisas

Arts & Sciences Graduate Student Theses and Dissertations

Hematopoiesis, the formation of blood and immune cells from stem and progenitor cells, is a central biological process that alters in response to the organismal state. Many cytokines that accompany diseases have an impact on the rate, cellular output, and underlying mechanisms of hematopoiesis. Deepening our understanding of the alterations to hematopoiesis that occur with disease may offer therapeutics that normalize hematopoiesis and promote health. One component of hematopoiesis that is often overlooked are the non-hematopoietic cells of the stem cell niche that have crucial roles in affecting the parameters of hematopoiesis. While progress in understanding the hematopoietic stem cell …

Effects Of Sex, Cadmium, And High-Fat-Diet On Accumulation Of Essential Metals In Mouse Kidneys., Justin Y. Xu, Adam Gaweda, Michelle Barati, Michael Merchant, Jun Cai, Lu Cai, Madhavi Rane

Undergraduate Research Events

Effects of cadmium and obesity on accumulation of essential metals in mouse kidney were evaluated. Starting in utero and continuing until 24 weeks postweaning, male/female mice were exposed to cadmium (0, 0.5, or 5 ppm) and were fed on Normal Diet (ND)/High-Fat Diet (HFD) post weaning. ICP-MS analysis was performed to evaluate cadmium and essential metals absorbed in the kidneys of aforementioned mice. Metals data obtained was analyzed using MANOVA with multiple comparisons (Bonferroni correction with p

How To Spark Bio Curiosity: An Innovative High School Biology Curriculum, Audrie Charles

Graduate Research Showcase

This project is a researched creative endeavor focused around the creation of an exemplary high school biology course. This presentation will showcase an original high school biology curriculum designed to ignite students’ innate curiosity and empower meaningful learning of core concepts like cell structure, genetics, evolution and ecology. Students revisit key ideas through a spiral curriculum that builds complexity, tackling open-ended problems and collaborating on hands-on inquiries like formulating hypotheses, designing experiments, collecting data and drawing evidence-based conclusions. Tangible projects allow students to demonstrate content knowledge in addition to critical thinking abilities like synthesizing concepts, evaluating claims and applying learning …

Quantifying Costs And Benefits Of Alternative Overwintering Strategies In A Southeastern Population Of Timber Rattlesnakes (Crotalus Horridus), John Powers, Amber Stubbs, Alfred J. Mead, Dominic L. Desantis

Graduate Research Showcase

Temperate reptile populations are under strong selective pressure to minimize the costs of extreme winter temperatures. For high latitude and high elevation snake populations, this has often favored communal overwintering behavior, wherein large numbers of conspecifics aggregate at optimal sites given the limited availability of such features in the environment. Timber Rattlesnakes (Crotalus horridus) are distributed across a wide latitudinal gradient in North America, and therefore represent ideal models for exploring how variable winter selective regimes might favor different strategies. Here, we leverage a unique intermediate population of C. horridus from central Georgia, USA, that exhibits both communal …

Hedgehog Signaling And Congenital Disorders; The Case Of Anencephaly And Polydactyly, Joshua Towery

First-Year Research Immersion (FRI) Scholarship

The Hedgehog pathway is a fundamental signaling pathway in the human body; involved in carcinogenesis, stem cell maintenance, as well as limb patterning in embryonic development. Originally discovered in drosophila, their initial discovery, and subsequent analysis revealed many novel pieces of information about the mechanisms inside the human body, as many core components of the signaling pathway are carried over from fruit flies to humans. Because of the key roles hedgehog signaling plays in human development, dysfunction and mutations in the pathway can be detrimental. This review will focus on two congenital birth defects thought to be caused by hedgehog …

The Plant Disease Triangle Facing Climate Change: A Molecular Perspective, Charles Roussin-Léveillée, Christina A. M. Rossi, Christian Castroverde, Peter Moffett

Biology Faculty Publications

No abstract provided.

Climate Change: An Existential Threat To Humanity, Brayden Asher Roy

Honors Projects in Biological and Biomedical Sciences

According to President Joe Biden, "Climate change is an existential threat to humanity" (Biden, 2020), one that requires the fullest attention from leaders within the international community. Research into the impacts of anthropogenic behavior on the climate began as early as the 1870s. When the clean energy movement generated support in the 1980s, fossil fuel companies responded to protect their existing business model that a transition to renewable energy would dismantle. Within the United States, through the suppression of information and lobbying of politicians, fossil fuel companies turned the concept of climate change into a partisan issue. Based on scientific …

Ccrg Soil Report, Cassandra Demlow, Cherith Pickett, David Ogboro

Student Papers and Reports

The goal of this project is to measure the ionic composition of a variety of soil samples, primarily using the ion chromatography technique. We also compared the ionic composition of multiple layers of the same sample.

Impacts Of Altitude On Plant Green Leaf, Fresh Litter, And Soil Stoichiometry In Subtropical Forests, Dehuang Zhu, Yan Zhou, Suhong Peng, Weiping Hua, Bin Luo, Dafeng Hui

Biology Faculty Research

BackgroundEcological stoichiometric characteristics of carbon (C), nitrogen (N), phosphorus (P), and potassium (K) serve as crucial indicators of nutrient cycling and limitation in terrestrial ecosystems. However, our current understanding of stoichiometric characteristics in subtropical forests and their response to different climate conditions is still limited.MethodsWe selected six altitudes ranging from 700 m to 1,200 m to simulate different climate conditions of an evergreen broadleaf forest in Wuyi Mountain, Fujian Province, China. We investigated C, N, P, and K stoichiometry and homeostasis in the green leaves, newly senesced leaf litter (fresh litter), and soil of this forest.ResultsLeaf P and K levels …

Investigation Of The Growth Of Listeria In Plant-Based Beverages, Klaudia Bartula, Sambou Biagui, Máire Begley, Michael Callanan

Department of Biological Sciences Publications

The objective of the present study was to evaluate whether the content of sugar, protein, fat, or fibre in commercially available and specially formulated plant-based beverages (oat, soya and pea) influences the growth rates of Listeria. Beverages were inoculated with a strain cocktail of Listeria (approximately 1 × 103 CFU/mL), and the data demonstrated that Listeria could proliferate in all tested beverages. Moreover, varying concentrations of naturally occurring or added sugar (0–3.3%), protein (3.3–5%), fat (1.1–3.5%) and added fibre (0–1.5%) did not have a statistically significant (p > 0.05) impact on the growth rates of Listeria in the tested …

Exploration Of Cytotoxicity Of _Rabidosa Rabida_ Venom On Invertebrate And Vertebrate Cell Cultures, Martina Gooden

Honors Theses

Venom has been observed in thousands of species across different taxa. It can be adapted for different purposes, including prey capture or predator defense. Venom is found almost universally in spiders, but there has been little exploration of spiders that are not medically significant. Rabidosa rabida, the rabid wolf spider, is a common species whose venom has not been adequately investigated. Previous studies indicate that they do not require venom to apprehend easy prey in a laboratory setting, raising a question as to what their venom is adapted to do. To evaluate its effect on mammalian cells, L929 murine fibroblasts …

Development Of A Novel Biology Laboratory Course For Incarcerated Students, Elsa H.K. Spencer, Joanna R. Vondrasek

Inquiry: The Journal of the Virginia Community Colleges

Federal second chance Pell grants were recently made widely available to incarcerated students in the United States to fund undergraduate education. Piedmont Virginia Community College was a pilot site for this expansion and began full scale implementation of transfer-oriented associate degrees at three correctional centers in its service area. The college did not have a biology laboratory curriculum that would meet general education requirements and be feasible in a correctional center, where students do not have internet access or even reliable computer (word processing or spreadsheet) access and where materials are restricted for entry. This paper will serve to introduce …

Varecia Rubra (Red Ruffed Lemur) Diel Activity And Calling At Andranobe, Masoala National Park, Madagascar, Hannah Elena Hilden-Reid

Dissertations and Theses

With respect to the natural variation in abiotic and biotic conditions present between daytime and nighttime periods, many animal species show evolutionary adaptations specialized for diurnality, nocturnality, or crepuscularity. Biologists have traditionally viewed categorizations of this kind as fixed within taxonomic groups, emphasizing how intricately species’ survival is contingent on the ability to adapt to low light, high light, or twilight conditions. However, movement away from such distinct temporal niche categories began within chronobiological studies following the discovery of more flexible patterns of activity in numerous taxa during the late 1970s. This shift generated increased interest in elucidating the complex …

Spiking Frequency Dependence On Input Current In Relation With The Leaky Integrate-And-Fire Neuron Model, Maria Chiara Cossio

Posters - 2024

Neurons are the most fundamental part of the nervous system and are diverse in structure and function containing a cell body, dendrites, and axons (Ludwig, et al., 2022). These electrically excitable cells receive and transmit electrical and chemical signals. These electrical impulses are known as action potentials in response to an input, and aids in the transmission of neurological information to neighboring neurons. An action potential (AP) is a rapid sequence of changes in the voltage across a membrane and it's an all-or-nothing event. It has three main stages: depolarization, repolarization, and hyperpolarization (Grider et al., 2023). Threshold depends on …

Influences Of Sex, Morph, And Weather On Roosting Distributions In Overwintering White-Throated Sparrows (_Zonotrichia Albicollis_), Rebecca Stephens

Honors Theses

Habitat choice influences survival by affecting access to resources, shelter from predators, mating opportunities, and numerous other costs and benefits. As such, habitat conservation is crucial, especially for migratory songbirds which are declining primarily due to habitat destruction. White-throated Sparrows (Zonotrichia albicollis) are a well-studied migratory songbird, but little is known about their roosting behaviors or how those behaviors are affected by sex, color morph, or weather. To determine this, we tracked White-throated Sparrows to their roosts, determined core home ranges, and analyzed the average distances of roost sites to the home range centroids according to these factors. …

Ecological Niche Modeling Of Endemic Texas Crayfish Species Under Current Conditions, Sarah A. Burgett

Biology Theses

Crayfish play many important roles in freshwater ecosystems such as ecosystem engineering, bioindicator, and food resource. Even though they are important to ecosystems, they are highly understudied. There has been previous work done on ecological niche modeling on Texas crayfish species, but this study takes it a step further by combining soil, climate, and hydrology variables to model the ecological niches of three endemic species: Procambarus texanus, Procambarus nueces, and Procambarus fayettei. Ecological niche models were created for the three species of interest using the program Maxent. I found that P. texanus and P. fayettei have indistinguishable …

New Genera And Species Of Caulobacter And Brevundimonas Bacteriophages Provide Insights Into Phage Genome Evolution, Bert Ely, Michael Hils, Aaron Clarke, Maegan Albert, Nadia Holness, Jacob Lenski, Tannaz Mohammadi

Faculty Publications

Previous studies have identified diverse bacteriophages that infect Caulobacter vibrioides strain CB15 ranging from small RNA phages to four genera of jumbo phages. In this study, we focus on 20 bacteriophages whose genomes range from 40 to 60 kb in length. Genome comparisons indicated that these diverse phages represent six Caulobacter phage genera and one additional genus that includes both Caulobacter and Brevundimonas phages. Within species, comparisons revealed that both single base changes and inserted or deleted genetic material cause the genomes of closely related phages to diverge. Among genera, the basic gene order and the orientation of key genes …

Rodent-Mediated Seed Limitation Affects Woody Seedling Establishment More Than Invasive Shrubs And Downed Woody Debris, Brian M. Connolly, Chad Zirbel, John L. Orrock

Biology Faculty Scholarship

Seedling establishment is crucial for the development of self-regenerating tree populations. Determinants of tree establishment vary widely and may compound in their effects. Using a factorial experiment, we manipulated invasive shrubs, downed woody debris (DWD), and rodent access to evaluate factors limiting the establishment of six woody species (five native trees, one invasive shrub). Our results suggest these factors independently contribute to tree seedling establishment. Exclusion of rodents increased establishment threefold. Invasive shrub removal (Elaeagnus umbellata; Lonicera maackii) and the presence of DWD promoted establishment of two native trees (Pinus strobus; Sassafras albidum). Notably, …

Parasite Diversity Of The King Cobra (Ophiophagus Hannah), Indian Rat Snake (Ptyas Mucosa), And Spectacled Cobra (Naja Naja), Bailey Tator

ALL - Honors Theses

Thus far, there has been little research on the parasitic biodiversity in wild reptiles, particularly in India. Research has been done on the parasite diversity of certain species, such as the King cobra (Ophiophagus hannah) and the Spectacled cobra (Naja naja), however these samples were purely from captive animals (Rajesh, 2015). This gap in knowledge is important to address because of how critical parasites are to the ecosystem and understanding their host population (Hudson, 2006). Parasites are some of the most biodiverse organisms, yet there is little work done to describe them in biodiversity hotspots such as the Western Ghats …