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Articles 3061 - 3090 of 39065

Full-Text Articles in Biology

The Potential Influence Of Immune Modulatory Molecules (Tgf-Βiii And Ctla-4) In Pathogenicity Of Pcos, Mustafa Riyadh Abdullah, Hazima Mossa Alabassi Jul 2024

The Potential Influence Of Immune Modulatory Molecules (Tgf-Βiii And Ctla-4) In Pathogenicity Of Pcos, Mustafa Riyadh Abdullah, Hazima Mossa Alabassi

Karbala International Journal of Modern Science

Polycystic ovary syndrome (PCOS) is characterised by chronic anovulation, hyperandrogenism, polycystic ovaries, and immunological dysregulation. Immune homeostasis and inflammation management require immune functions. T-regulatory cells (Tregs) regulate PCOS's immune system, inflammation, insulin resistance, ovarian function, and homeostasis. Transforming growth factor βIII (TGF-βIII) and Cytotoxic-T-lymphocyte-associated-protein-4 (CTLA-4) receptors on the surfaces of Treg cells are important for controlling the immune system. The study included 68 PCOS patients and 22 non-PCOS women controls aged 20–45. The waist-hip ratio (WHR) determines obesity. We evaluated serum levels and gene expression of TGF-βIII and CTLA-4 via ELISA and real-time PCR. Women with PCOS had significantly higher …


Enhanced Optoelectronics Performance Of Hybrid Self Power Photodetectors Go: Tio2- Ad / N-Si Heterojunctions, Mohammed J. Alsultani, Maysoon F. Alias Jul 2024

Enhanced Optoelectronics Performance Of Hybrid Self Power Photodetectors Go: Tio2- Ad / N-Si Heterojunctions, Mohammed J. Alsultani, Maysoon F. Alias

Karbala International Journal of Modern Science

A GO:TiO2/n-Si heterostructure has been submerged in an anthocyanin dye solution that extracted from the red cabbage plant creates a high responsivity, self-powered UV, and visible photodetectors fabricated by the spray pyrolysis technique. The GO concentrations are varied, whereas TiO2 is fixed. Thin films' structure, shape, and optical characteristics were examined using X-ray diffraction, field emission scanning electron microscopy, and UV-Vis spectrophotometers respectively. Island-like polycrystalline film powders with grain boundaries and granular shapes are created. Two direct energy gaps between 3.33-3.02 and 2.39-2.04 eV exist in all films. J-V characteristics were examined. The saturation current density (J …


Fungi Of Lambuth: A Field Guide, Kelly Maust, Shawn P. Brown, Elizabeth Weston Jul 2024

Fungi Of Lambuth: A Field Guide, Kelly Maust, Shawn P. Brown, Elizabeth Weston

Open Access Textbooks

Fungi of Lambuth is an open-access, beginner-level field guide designed to introduce University of Memphis, Lambuth faculty, staff, and students to the joys of mushroom hunting, and to provide an overview of the types of mushrooms that can be found right on our campus (located at 705 Lambuth Blvd. in Jackson, TN).


Genetics Of Mdh In Humans, Adam Haberman, Celeste N. Peterson Jul 2024

Genetics Of Mdh In Humans, Adam Haberman, Celeste N. Peterson

Biology: Faculty Scholarship

Malate dehydrogenase (MDH) performs key roles in metabolism, but little is known about its function specifically in human health and disease. In this minireview, we describe the incomplete state of our knowledge of human MDH genetics. Humans have three MDH genes with a total of four validated isoforms. MDH1 and MDH2 are widely expressed, while MDH1B is only expressed in a small subset of tissues. Many mutations in MDH1 and MDH2 have been identified in patients, but only a few have been studied to determine what symptoms they cause. MDH1 has been associated with cancer and a neurodevelopmental disorder. MDH2 …


Draft Genome Assemblies Of One Lactobacillus Gasseri Strain And Two Lactobacillus Jensenii Strains Isolated From Voided Urine Samples, Alex Kula, Natalie Stegman, Catherine Putonti Jul 2024

Draft Genome Assemblies Of One Lactobacillus Gasseri Strain And Two Lactobacillus Jensenii Strains Isolated From Voided Urine Samples, Alex Kula, Natalie Stegman, Catherine Putonti

Biology: Faculty Publications and Other Works

We present the draft genome for three Lactobacillus strains isolated from female urine specimens: Lactobacillus gasseri UMB1673, Lactobacillus jensenii UMB1855, and Lactobacillus jensenii UMB5069. Focusing on strains within the female urinary microbiome can provide a more well-rounded understanding of the microbial community and its influence on health and disease.


Tissue-Specific Effects Of Temperature And Salinity On The Cell Cycle And Apoptosis In The Nile Tilapia (Oreochromis Niloticus)., Rachel M. Palmer, Arika Sandbach, Bradley A. Buckley Jul 2024

Tissue-Specific Effects Of Temperature And Salinity On The Cell Cycle And Apoptosis In The Nile Tilapia (Oreochromis Niloticus)., Rachel M. Palmer, Arika Sandbach, Bradley A. Buckley

Biology Faculty Publications and Presentations

The Nile Tilapia (Oreochromis niloticus) evolved in warm, freshwater rivers, but possesses a broad physiological tolerance to a range of environmental conditions. Due to this hardiness and resilience, this species has been successfully introduced to regions widely outside of its native range. Here, we examine the impact of temperature and salinity variation on this species at the sub-lethal level. Specifically, Nile Tilapia were exposed to two temperatures (21 °C or 14 °C) and three salinities (0, 16, 34 ppt) for 1-h. Given their native habitat, the 21 °C / 0 ppt exposure was considered the control condition. Both cell cycle …


The Value Of Using Validated Competency-Based Assessments Across The Career Span Of An Athletic Trainer, Matthew Drescher, Lindsey Eberman Jul 2024

The Value Of Using Validated Competency-Based Assessments Across The Career Span Of An Athletic Trainer, Matthew Drescher, Lindsey Eberman

Clinical Practice in Athletic Training

No abstract provided.


Perceptions And Use Of The Athletic Training Milestones In Education: A Report From The Aate Research Network, Cailee E. Welch Bacon, Barton E. Anderson, Julie M. Cavallario, Bonnie L. Van Lunen, Lindsey E. Eberman Jul 2024

Perceptions And Use Of The Athletic Training Milestones In Education: A Report From The Aate Research Network, Cailee E. Welch Bacon, Barton E. Anderson, Julie M. Cavallario, Bonnie L. Van Lunen, Lindsey E. Eberman

Clinical Practice in Athletic Training

The Athletic Training Milestones (AT Milestones) were developed as an evaluation tool to capture the breadth and depth of athletic training knowledge, skills, and behavior. However, it is unclear whether athletic training programs are implementing this tool or how they are implementing it to gauge the clinical progression of students or residents in athletic training. We aimed to explore the perceptions and use of the AT Milestones among educators in athletic training programs. We used a cross-sectional, web- based survey with open-ended questions to collect data from athletic training programs on if and how they implement the AT Milestones at …


Standardized Patient Evaluation Tool: A Valid Measure Of The Core Competencies, Lindsey E. Eberman, Jessica Edler Nye Jul 2024

Standardized Patient Evaluation Tool: A Valid Measure Of The Core Competencies, Lindsey E. Eberman, Jessica Edler Nye

Clinical Practice in Athletic Training

No abstract provided.


The Development, Implementation, And Value Of The Athletic Training Milestones For Assessing Progressive Clinical Behaviors, Hollie Walusz, Eric Sauers, Mark Laursen, Forrest Pecha Jul 2024

The Development, Implementation, And Value Of The Athletic Training Milestones For Assessing Progressive Clinical Behaviors, Hollie Walusz, Eric Sauers, Mark Laursen, Forrest Pecha

Clinical Practice in Athletic Training

No abstract provided.


Microrna166: Old Players And New Insights Into Crop Agronomic Traits Improvement, Zhanhui Zhang, Tianxiao Yang, Na Li, Guiliang Tang, Jihua Tang Jul 2024

Microrna166: Old Players And New Insights Into Crop Agronomic Traits Improvement, Zhanhui Zhang, Tianxiao Yang, Na Li, Guiliang Tang, Jihua Tang

Michigan Tech Publications

MicroRNA (miRNA), a type of non-coding RNA, is crucial for controlling gene expression. Among the various miRNA families, miR166 stands out as a highly conserved group found in both model and crop plants. It plays a key role in regulating a wide range of developmental and environmental responses. In this review, we explore the diverse sequences of in major crops and discuss the important regulatory functions of miR166 in plant growth and stress responses. Additionally, we summarize how miR166 interacts with other miRNAs and highlight the potential for enhancing agronomic traits by manipulating the expression of miR166 and its targeted …


An Exploration Of The Drivers Of Intraspecific Variation In Arthropod Phenotypes, Jamie Phelps Jul 2024

An Exploration Of The Drivers Of Intraspecific Variation In Arthropod Phenotypes, Jamie Phelps

LSU Doctoral Dissertations

Herein we explore the various forces that drive phenotypic variation in arthropods focusing on spatial and temporal differences as well as sexual dimorphism. We use both marine, Tigriopus californicus, and terrestrial, Melitaeini, organisms to investigate these forces.

Tigriopus californicus and Heat Hardening: The study explores how Tigriopus californicus, a copepod, adapts to thermal variation along the North American Pacific Coast. Researchers investigated intraspecific variation in heat hardening, measuring differences in upper lethal limits (ULLs) and potential fecundity costs across five populations. We found a cost of heat hardening: the size of the first brood produced after heat shock …


Membrane Vectorial Lipidomic Features Of Coral Host Cells’ Plasma Membrane And Lipid Profiles Of Their Endosymbionts Cladocopium, Tatyana V Sikorskaya, Ekaterina V Ermolenko, Taliya T Ginanova, Andrey V Boroda, Kseniya V Efimova, Mikhail Bogdanov Jul 2024

Membrane Vectorial Lipidomic Features Of Coral Host Cells’ Plasma Membrane And Lipid Profiles Of Their Endosymbionts Cladocopium, Tatyana V Sikorskaya, Ekaterina V Ermolenko, Taliya T Ginanova, Andrey V Boroda, Kseniya V Efimova, Mikhail Bogdanov

Faculty, Staff and Student Publications

The symbiotic relationships between coral animal host and autotrophic dinoflagellates are based on the mutual exchange and tight control of nutritional inputs supporting successful growth. The corals Sinularia heterospiculata and Acropora aspera were cultivated using a flow-through circulation system supplying seawater during cold and warm seasons of the year, then sorted into host cells and symbionts and subjected to phylogenetic, morphological, and advanced lipid analyses. Here we show, that the lipidomes of the dinoflagellates Cladocopium C1/C3 and acroporide-specific Cladocopium hosted by the corals, are determined by lipidomic features of different thermosensitivity and unique betaine- and phospholipid molecular species. Phosphatidylserines and …


Single-Fly Genome Assemblies Fill Major Phylogenomic Gaps Across The Drosophilidae Tree Of Life, Bernard Y. Kim, Hannah R. Gellert, Samuel H. Church, Anton Suvorov, Sean S. Anderson, Olga Barmina, Sofia G. Beskid, Aaron A. Comeault, K. Nicole Crown, Sarah E. Diamond, Steve Dorus, Takako Fujichika, James A. Hemker, Jan Hrcek, Thomas Werner, Et. Al Jul 2024

Single-Fly Genome Assemblies Fill Major Phylogenomic Gaps Across The Drosophilidae Tree Of Life, Bernard Y. Kim, Hannah R. Gellert, Samuel H. Church, Anton Suvorov, Sean S. Anderson, Olga Barmina, Sofia G. Beskid, Aaron A. Comeault, K. Nicole Crown, Sarah E. Diamond, Steve Dorus, Takako Fujichika, James A. Hemker, Jan Hrcek, Thomas Werner, Et. Al

Michigan Tech Publications

AU Long-read: Pleaseconfirmthatallheadinglevelsarerepresentedcorrectly sequencing is driving rapid progress in genome assembly : across all major groups of life, including species of the family Drosophilidae, a longtime model system for genetics, genomics, and evolution. We previously developed a cost-effective hybrid Oxford Nanopore (ONT) long-read and Illumina short-read sequencing approach and used it to assemble 101 drosophilid genomes from laboratory cultures, greatly increasing the number of genome assemblies for this taxonomic group. The next major challenge is to address the laboratory culture bias in taxon sampling by sequencing genomes of species that cannot easily be reared in the lab. Here, we build …


Risk Of Invasive Waterfowl Interaction With Poultry Production: Understanding Potential For Avian Pathogen Transmission Via Species Distribution Models, Reilly T. Jackson, Percival Matzinger Marshall, Chris R. Burkhart, Julia Schneck, Grant Kelly, Caleb P. Roberts Jul 2024

Risk Of Invasive Waterfowl Interaction With Poultry Production: Understanding Potential For Avian Pathogen Transmission Via Species Distribution Models, Reilly T. Jackson, Percival Matzinger Marshall, Chris R. Burkhart, Julia Schneck, Grant Kelly, Caleb P. Roberts

Biological Sciences Faculty Publications and Presentations

Recent outbreaks of highly pathogenic avian influenza have devastated poultry production across the United States, with more than 77 million birds culled in 2022–2024 alone. Wild waterfowl, including various invasive species, host numerous pathogens, including highly pathogenic avian influenza virus (HPAIV), and have been implicated as catalysts of disease outbreaks among native fauna and domestic birds. In major poultry-producing states like Arkansas, USA, where the poultry sector is responsible for significant economic activity (>$4 billion USD in 2022), understanding the risk of invasive waterfowl interactions with domestic poultry is critical. Here, we assessed the risk of invasive waterfowl-poultry interaction …


Seed Dispersal By Martu Peoples Promotes The Distribution Of Native Plants In Arid Australia, Rebecca Bliege Bird, Douglas Bird, Christopher T. Martine, Chloe Mcguire, Leanne Greenwood, Desmond Taylor, Tanisha M. Williams, Peter M. Veth Jul 2024

Seed Dispersal By Martu Peoples Promotes The Distribution Of Native Plants In Arid Australia, Rebecca Bliege Bird, Douglas Bird, Christopher T. Martine, Chloe Mcguire, Leanne Greenwood, Desmond Taylor, Tanisha M. Williams, Peter M. Veth

Faculty Journal Articles

Commensal relationships between wild plants and their dispersers play a key ecological and evolutionary role in community structure and function. While non-human dispersers are often considered critical to plant recruitment, human dispersers have received much less attention, especially when it comes to non-domesticated plants. Australia, as a continent historically characterized by economies reliant on non-domesticated plants, is thus a key system for exploring the ecological role of people as seed dispersers in the absence of agriculture. Here, we utilize a controlled observation research design, employing ecological surveys and ethnographic observations to examine how seed dispersal and landscape burning by Martu …


Host-Microbiome Interactions In The Teleost Brain, Amir Mani Varnoosfaderani Jul 2024

Host-Microbiome Interactions In The Teleost Brain, Amir Mani Varnoosfaderani

Biology ETDs

This dissertation investigates host-microbial interactions within the teleost brain, previously considered sterile. Reviewing current literature highlights significant gaps in understanding teleost brain immunity, despite prevalent neurotropic diseases. We mapped the immune profiles of rainbow trout brains and meninges, revealing the olfactory bulbs as the immune effector part of the brain and the optic tectum as a nod2 expression hub. High il6 and il10 levels were noted in the telencephalon. A persistent bacterial community within healthy salmonid brains shares diversity with gut and blood microbiota. Brain bacterial isolates exhibit genomic signatures for brain colonization and polyamine biosynthesis. The microbiota shifts across …


Records Of The Red-Bellied Snake (Storeria Occipitomaculata) In Central And Northeastern Nebraska, Taylor Lowe, Sarah M. Bailey, Dan D. Fogell, Keith Geluso Jul 2024

Records Of The Red-Bellied Snake (Storeria Occipitomaculata) In Central And Northeastern Nebraska, Taylor Lowe, Sarah M. Bailey, Dan D. Fogell, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Red-bellied Snakes (Storeria occipitomaculata) are a distinctive, small species that live in moist woodlands throughout much of eastern North America. In the Great Plains, isolated populations exist in South Dakota, Wyoming, and Nebraska. In Nebraska, the only known extant population occurs in wooded riparian habitats along the Big Bend Reach of the Platte River in southcentral parts of the state. Herein, we report on new county records of Red-bellied Snakes in Nebraska, with two observations from Merrick County along the Platte River adjacent to known populations and two observations of a new population in the extreme northeastern part …


Application Of Automated Radio Telemetry To Explore Space Use Of Golden-Cheeked Warblers (Setophaga Chrysoparia), Michael Gamble Jul 2024

Application Of Automated Radio Telemetry To Explore Space Use Of Golden-Cheeked Warblers (Setophaga Chrysoparia), Michael Gamble

LSU Master's Theses

Understanding the spatiotemporal ecology of birds is essential to inform conservation and management decisions that could help maintain bird populations and reverse current population trends. Advancements in automated radio telemetry systems (ARTS) may improve our ability to monitor animal movements continuously and simultaneously but require further testing and development to maximize their benefits for wildlife research. My goals were (1) to investigate the use of automated telemetry to track fine-scale songbird movements in a forested environment, and (2) to use automated telemetry to quantify home range size, spatial overlap between home ranges, and extraterritorial forays for male and female golden-cheeked …


Examining The Oxidation States Of Metals In Aerosols Emitted By Electronic Cigarettes, Kashala Fabrice Kapiamba, Stephen Yaw Owusu, Yangtao Wu, Yue-Wern Huang, Yi Jiang, Yang Wang Jul 2024

Examining The Oxidation States Of Metals In Aerosols Emitted By Electronic Cigarettes, Kashala Fabrice Kapiamba, Stephen Yaw Owusu, Yangtao Wu, Yue-Wern Huang, Yi Jiang, Yang Wang

Biological Sciences Faculty Research & Creative Works

Electronic cigarettes (ECs) emit many toxic substances, including metals, that can pose a threat to users and the environment. The toxicity of the emitted metals depends on their oxidation states. Hence, this study examines the oxidation states of metals observed in EC aerosols. X-ray photoelectron spectroscopy analysis of the filters that collected EC aerosols identified the oxidation states of five primary metals (based on surface sample analysis), including chromium(III) (close to 100%) under low power setting while a noticeable amount of chromium(VI) (15%) at higher power settings of the EC, and copper(II) (100%), zinc(II) (100%), nickel(II) (100%), lead(II) (65%), and …


Short-Term Time-Series Observations Of Phytoplankton Light-Absorption And Productivity In Prydz Bay, Coastal Antarctica, Sarat C. Tripathy, Anvita U. Kerkar, P. Sabu, Sunil K. Padhi, Sudarsana R. Pandi, Amit Sarkar, Bhaskar V. Parli, Rahul Mohan Jul 2024

Short-Term Time-Series Observations Of Phytoplankton Light-Absorption And Productivity In Prydz Bay, Coastal Antarctica, Sarat C. Tripathy, Anvita U. Kerkar, P. Sabu, Sunil K. Padhi, Sudarsana R. Pandi, Amit Sarkar, Bhaskar V. Parli, Rahul Mohan

Biology Faculty Publications and Presentations

The optical characteristics of coastal Antarctic waters exhibit complexity due to the dynamic hydrography influenced by meltwater intrusion, which alters nutrient levels, thermohaline structure, and optically active substances (OAS) regimes. Studies on bio-optical variability and its implications on phytoplankton productivity (PP) are scanty in coastal polar regions. On this backdrop, time-series measurements (72 h at 6 h intervals) of bio-optical properties such as phytoplankton biomass (chlorophyll-a), absorption (aph), and total suspended matter (TSM) concurrently with PP were measured to understand their interplay and variability in relation to the ambient physicochemical settings in the under-sampled Prydz Bay, coastal Antarctica. Our findings …


Support For Climate Shaping The Mitochondrial Dna Contact Zone In The Audubon's Warbler, Kyana Nina Montoya Jul 2024

Support For Climate Shaping The Mitochondrial Dna Contact Zone In The Audubon's Warbler, Kyana Nina Montoya

Biology ETDs

Mitochondrial introgression has potential consequences for physiology and adaptation, but its causes are poorly understood. The Audubon’s Warbler (AUWA; Setophaga coronata auduboni) provides a case study to examine those causes as mitochondrial introgression has swept through part of the range. The northern mitochondrial genome (mtDNA) introgressed from the Myrtle Warbler (MYWA; S. c. coronata) and is 4% divergent from the southern mtDNA. Incomplete introgression suggests discordant AUWAs conferred a selective advantage in the north; it was hypothesized that energetic demands associated with migration formed this advantage. We tested two hypotheses to explain partial introgression and the mtDNA distribution …


Ponderosa Pine Plant Community Response To High-Severity Wildfire In The Jemez Mountains, Samantha Brodley Jul 2024

Ponderosa Pine Plant Community Response To High-Severity Wildfire In The Jemez Mountains, Samantha Brodley

Biology ETDs

Climate change related temperature increases, and precipitation shifts, along with an increase in fuel loads have rendered large areas of the Southwest more prone to high-severity fire for longer periods of time throughout each year. Fire adapted forests that experience high-severity, stand replacing fires are not consistently experiencing dominant tree species regeneration, and appear, in some cases, to be shifting into novel systems. We collected and analyzed up to 11 years of understory plant community data in ponderosa pine (Pinus ponderosa) forests in the Jemez Mountains, New Mexico, to address early forest regeneration after high and low severity …


Conservation Genomic Investigation Of A Threatened Trout Species Endemic To The Gila River, David Thomas Camak Jul 2024

Conservation Genomic Investigation Of A Threatened Trout Species Endemic To The Gila River, David Thomas Camak

Biology ETDs

Climate change is a looming threat to the persistence of species, especially aquatic, imperiled species. Species native to desert streams are at a particular disadvantage, as many species already face drought, floods, wildfires, and threats from nonnative species, all of which are thought to be exacerbated by climate change. Species such as the threatened Gila Trout (Oncorhynchus gilae) already have small ranges and persist in fragmented and isolated habitats that limits gene flow between populations. Therefore, it is important to understand the current genomic status of such populations to be able to predict the future effects of climate …


Otocolobus Manul (Carnivora: Felidae), Paula Noonan, Zoe Baker, Virginia Hayssen Jul 2024

Otocolobus Manul (Carnivora: Felidae), Paula Noonan, Zoe Baker, Virginia Hayssen

Biological Sciences: Faculty Publications

Otocolobus manul (Pallas, 1776) is a small, solitary, terrestrial felid commonly called the Manul and is the sole species of its genus. It occurs in Afghanistan, Armenia, Bhutan, China, India, the Islamic Republic of Iran, Kazakhstan, Kyrgyzstan, Mongolia, Nepal, Pakistan, the Russian Federation, and Turkmenistan. Otocolobus manul might still occur in Tajikistan and Uzbekistan but might be extinct in Azerbaijan. As assessed in 2020, O. manul is listed as “Least Concern” (LC) on the International Union for Conservation of Nature and Natural Resources Red List of Threatened Species, with an estimate of 58,000 adults, although the population might be decreasing.


Lymphoid Tissues Contribute To Plasma Viral Clonotypes Early After Antiretroviral Therapy Interruption In Siv-Infected Rhesus Macaques, Antonio Solis-Leal, Nongthombam Boby, Suvadip Mallick, Yilun Cheng, Fei Wu, Grey De La Torre, Jason Dufour, Xavier Alvarez, Vinay Shivanna, Yaozhong Liu, Christine M. Fennessey, Jeffrey D. Lifson, Qingsheng Li, Brandon F. Keele, Binhua Ling Jul 2024

Lymphoid Tissues Contribute To Plasma Viral Clonotypes Early After Antiretroviral Therapy Interruption In Siv-Infected Rhesus Macaques, Antonio Solis-Leal, Nongthombam Boby, Suvadip Mallick, Yilun Cheng, Fei Wu, Grey De La Torre, Jason Dufour, Xavier Alvarez, Vinay Shivanna, Yaozhong Liu, Christine M. Fennessey, Jeffrey D. Lifson, Qingsheng Li, Brandon F. Keele, Binhua Ling

School of Biological Sciences: Faculty Publications

The rebound-competent viral reservoir, composed of a virus that is able to persist during antiretroviral therapy (ART) and mediate reactivation of systemic viral replication and rebound viremia after ART interruption (ATI), remains the biggest obstacle to treating HIV infection. A better understanding of the cellular and tissue origins and the dynamics of viral populations that initiate rebound upon ATI could help develop therapeutic strategies for reducing the rebound-competent viral reservoir. In this study, barcoded simian immunodeficiency virus (SIV), SIVmac239M, was used to infect rhesus macaques to enable monitoring of viral barcode clonotypes contributing to virus detectable in plasma after ATI. …


Role Of Sds-22/Pp1 Phosphatase In Polarizing The Germplasm Of Caenorhabditis Elegans Embryo, Aparna Nurni Ravi Jul 2024

Role Of Sds-22/Pp1 Phosphatase In Polarizing The Germplasm Of Caenorhabditis Elegans Embryo, Aparna Nurni Ravi

Dartmouth College Ph.D Dissertations

During asymmetric cell division, cell polarity and cell cycle are tightly coupled to ensure robust segregation of cell fate determinants and generation of cellular diversity. In the one-cell C. elegans embryo, multiple cell cycle kinases regulate cell polarization and posterior segregation of germline fate determinants (germplasm). For example, PLK-1 kinase inhibits the retention of its germplasm substrate POS-1 in the anterior, driving POS-1 segregation to the posterior (Han et al., 2018). Additionally, MBK-2 kinase disassembles P granules in the anterior through phosphorylation of the P granule scaffold MEG-3, helping to drive P granule segregation to the posterior (Wang et al., …


Inter-Kingdom Communication And The Sympoietic Way Of Life, Scott F. Gilbert Jul 2024

Inter-Kingdom Communication And The Sympoietic Way Of Life, Scott F. Gilbert

Biology Faculty Works

Organisms are now seen as holobionts, consortia of several species that interact metabolically such that they sustain and scaffold each other’s existence and propagation. Sympoiesis, the development of the symbiotic relationships that form holobionts, is critical for our understanding the origins and maintenance of biodiversity. Rather than being the read-out of a single genome, development has been found to be sympoietic, based on multigenomic interactions between zygote-derived cells and symbiotic microbes. These symbiotic and sympoietic interactions are predicated on the ability of cells from different kingdoms of life (e.g., bacteria and animals) to communicate with one another and to have …


Improved Whole-Mount Immunofluorescence Protocol For Consistent And Robust Labeling Of Adult Drosophilamelanogaster Adipose Tissue, Rachael K. Ott, Isaiah H. Williams, Alissa R. Armstrong Jul 2024

Improved Whole-Mount Immunofluorescence Protocol For Consistent And Robust Labeling Of Adult Drosophilamelanogaster Adipose Tissue, Rachael K. Ott, Isaiah H. Williams, Alissa R. Armstrong

Faculty Publications

Energy storage and endocrine functions of the Drosophila fat body make it an excellent model for elucidating mechanisms that underlie physiological and pathophysiological organismal metabolism. Combined with Drosophila's robust genetic and immunofluorescence microscopy toolkits, studies of Drosophila fat body function are ripe for cell biological analysis. Unlike the larval fat body, which is easily removed as a single, cohesive sheet of tissue, isolating intact adult fat body proves to be more challenging, thus hindering consistent immunofluorescence labeling even within a single piece of adipose tissue. Here, we describe an improved approach to handling Drosophila abdomens that ensures full access of …


Development Of A Simple And Accurate Polymerase Chain Reaction Screen For Human Papillomavirus In College Students, Emily Bishop, Owen Smith, Paul E. Richardson Jul 2024

Development Of A Simple And Accurate Polymerase Chain Reaction Screen For Human Papillomavirus In College Students, Emily Bishop, Owen Smith, Paul E. Richardson

Journal of the South Carolina Academy of Science

Human Papillomavirus (HPV) is, according to the CDC, the most common sexually transmitted infection (STI) with many infections developing in a person's late teens or early 20’s. HPV is a nonenveloped, relatively small, icosahedral DNA virus that infects squamous epithelial cells. This infection can happen in genital, anal, and oral cavities. 90% of these infections will self-clear in two years according to the CDC. Of the 10% of infections that do not clear in two years, they could develop into carcinomas later in life. The goal of this developmental research is to create a polymerase chain reaction (PCR) that can …