Biology Commons^™

Simulating Interventions To Improve Reproducibility In Scientific Publications, Ben G. Fitzpatrick

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Bodine: An Agent-Based Modeling Approach To Studying Localized Coexistence Across Environmental Heterogeneity, Alexandra Campbell

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Comparison Of Efficient Deep Learning Architectures For Lactobacillus Species Identification, Dea Aisyah Rusmawati, Ishak Ariawan, Afrinal Firmanda

Karbala International Journal of Modern Science

Identifying microorganism species, such as Lactobacillus, is essential in ensuring the food products' quality and safety. Traditional laboratory practice requires expert knowledge and experience, but the method is expensive and time-consuming due to complex sample preparation. Faster, more accurate, and cheaper computational methods, such as transfer learning technology, are needed for the Lactobacillus species classification. The technique has been effective in a variety of image recognition contexts. Deep learning architecture can also be applied as an innovative strategy for digital image-based identification. Therefore, this research aims to compare several deep-learning architectures in classifying bacterial strains of Lactobacillus. The four architectures …

Role Of B Cell Mediated Immune Responses During Skin ‘Atopic Dermatitis’ Disease, Hidaya Mohammed Abdul Kader

Thesis/ Dissertation Defenses

Atopic dermatitis (AD) is the most prevalent non-infectious skin allergies with approximately 10-30% adults and 40% children being affected worldwide. It is clinically characterized by eczema tic lesions and elevated IgE levels and is a multifactorial disease that is heterogeneous in nature. AD results in a dominant Th2 polarized immune response. Until date, there is no cure for this disease. B cells are key players of humoral immune responses and contribute significantly to allergic responses through IgE secretion. However, recently a subset of B cells termed regulatory B cells (Bregs) were discovered that were shown to have a protective role …

Anticancer Activity Of Capparis Spinosa Extract Against Colorectal Cancer, Shamaa Abdul Samad

Thesis/ Dissertation Defenses

Colorectal cancer (CRC) is one of the most frequently diagnosed cancers in the world. Plants are becoming a rich source of new anticancer compounds with novel targets. In this study, we evaluated the anticancer effects of Capparis spinosa on two CRC cell lines. HCT-116 and HT-29 CRC cells treated with various concentrations of C. spinosa leaf extract (CSLE) demonstrated inhibition of proliferation in a concentration-dependent manner. Additional analyses such as Western blot analysis, colony forming assay, and wound healing assay were performed to elucidate the mechanisms and pathways involved. The results revealed that non-cytotoxic concentrations of CSLE significantly inhibited the …

Assessing The Impact Of Irrigation Water And Soil Salinity On Date Palm And Desert Soil Associated Microbiota, Subha Chandran

Thesis/ Dissertation Defenses

Soil salinization poses a critical challenge in arid and semi-arid regions, where water scarcity is exacerbated by climate change and unsustainable agricultural practices. This phenomenon not only reduces soil fertility but also significantly impacts groundwater quality, leading to detrimental effects on crop yields and ecosystem health. Date palm (Phoenix dactylifera) cultivation, a key agricultural practice in many arid regions, often relies on saline groundwater for irrigation due to limited freshwater resources and low precipitation rates. The use of saline water can create an intricate balance between providing necessary nutrients and introducing harmful salts into the soil. While the role of …

Red Snapper Neurocranium, Roman Devlin, Michael A. Birnbaum, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Diverse Genome Structures Among Eukaryotes May Have Arisen In Response To Genetic Conflict, Elinor G. Sterner, Auden Cote-L’Heureux, Xyrus X. Maurer-Alcalá, Laura A. Katz

Biological Sciences: Faculty Publications

In contrast to the typified view of genome cycling only between haploidy and diploidy, there is evidence from across the tree of life of genome dynamics that alter both copy number (i.e. ploidy) and chromosome complements. Here, we highlight examples of such processes, including endoreplication, aneuploidy, inheritance of extrachromosomal DNA, and chromatin extrusion. Synthesizing data on eukaryotic genome dynamics in diverse extant lineages suggests the possibility that such processes were present before the last eukaryotic common ancestor. While present in some prokaryotes, these features appear exaggerated in eukaryotes where they are regulated by eukaryote-specific innovations including the nucleus, complex cytoskeleton, …

Boosting The Power Performance Of Microbial Fuel Cells By Using Dual Nanomaterial-Modified Carbon Felt Electrodes, Krishna Thapa, Wenyan Liu, Yuwei Zhang, David J. Westenberg, Yishu Zhou, Risheng Wang

Chemistry Faculty Research & Creative Works

Innovative design enhancements to microbial fuel cells (MFCs) are pivotal for improving their viability as renewable energy sources. This study introduces a dual approach to electrode modification, integrating polyaniline (PANI) and gold nanoparticles (AuNPs) on carbon felt (CF) anodes coupled with a unique cathode composed of DNA origami and chitosan. The synergistic effect of PANI and AuNPs significantly increases the hydrophilicity and conductivity of the anode, leading to a considerable surge in power density. Concurrently, the biocompatible DNA origami and chitosan-functionalized cathode facilitates efficient electron transfer without relying on conventional catalysts. Together, these modifications yield a 52.42% increase in the …

Avian Index Of Biotic Integrity: Evaluating Wetland Health In The Red River Basin, Texas/Louisiana, Elexis M. Anderson

Biology Theses

Wetlands play a vital role in maintaining environmental health by filtering pollutants, controlling floods, and providing habitat for diverse species. These ecosystems are essential for sustaining biodiversity and regulating hydrological and biogeochemical cycles. The Red River Basin (The Red River of the South), spanning Texas, Oklahoma, and Louisiana, is home to various types of wetlands, which provide essential habitats to an array of wildlife. However, anthropogenic pressures such as agriculture and urbanization have led to the degradation and loss of these valuable ecosystems. To counter this loss, federal programs such as the Agricultural Conservation Easement Program - Wetland Reserve Easement …

Clock Hijackers: How Integration Of Glioblastoma Into Circadian Circuits Regulates Tumor Progression And Sensitivity To Current Therapies, Maria Felicita Gonzalez

Arts & Sciences Graduate Student Theses and Dissertations

Glioblastoma (GBM) is the most common primary brain tumor in adults with a poor prognosis despite aggressive therapy. A recent, retrospective clinical study found that administering chemotherapy with Temozolomide (TMZ) in the morning increased patient overall survival by 6 months compared to evening. Previous work has shown that murine and human models of GBM have cell-intrinsic circadian rhythms in expression of the core clock genes Bmal1 and Per2 in vitro. This suggests that GBM has circadian rhythms that entrain to the hosts’ central clock. Understanding the mechanisms underlying circadian entrainment and tumor growth in GBM is essential to determine whether …

Classification Manner Utilizing Electroencephalography Signals To Investigate Waveforms, Wessam Al-Salman, Ali Basim Al-Khafaji, Mishall Al-Zubaidie

Karbala International Journal of Modern Science

Waveform detection has been an area of continuous investigation for many years. One important waveform in sleep stage 2 is the k-complex. Numerous researchers have created various strategies for auto-k-complex detection; some of these strategies state that the automated detection techniques are adequate. Because of its analytically relevant resolution, the Electroencephalogram (EEG) is a commonly utilized technique to analyze the k-complexes in order to understand the nervous system activity of the brain. Several researchers have classified waveforms using EEGs in a variety of ways. It appears that the majority of the waveform detection had limitations. The …

Bacterial Cellulose Production From Fermented Fruits And Vegetables Byproducts: A Comprehensive Study On Chemical And Morphological Properties, Yati Maryati, Hakiki Melanie, Windri Handayani, Yasman Yasman

Karbala International Journal of Modern Science

This study aimed to produce α-cellulose from Bacterial Cellulose SCOBY (BCS) using alternative substrates from fermented fruit and vegetable byproducts: katuk leaves (KT), kale leaves (KL), guava (JB), dragon fruit (NG), and banana (PS). BCS production involved juice extraction, SCOBY inoculation, and sucrose addition, followed by 21 days of fermentation. Initially, the NG medium had the highest concentration of Total Reducing Sugars (TRS), but all media showed a decline as sugars were consumed. Fermentation reduced pH and increased total polyphenols, with KL and JB showing the highest rise (0.13-0.15 mg GAE/mL). Flavonoid levels varied, decreasing in KL and PS but …

Metabolite Profiling Of Potential Fraction From Ethyl Acetate Extract Of Ziziphus Mauritiana Leaves By Lc-Ms/Ms Analysis, Nurhayati Bialangi, Weny Ja Musa, Boima Situmeang

Karbala International Journal of Modern Science

In Indonesia, the treatment with leaves as a traditional medicine is still firmly integrated into the community to overcome various health problems experienced, one of which is the treatment with Bidara leaves (Ziziphus mauritiana). Ziziphus mauritiana belongs to family of Rhamnaceae, and it is generally considered a potential source of antioxidant and cholesterol lowering. This study aimed to examine phytochemical characterization, antioxidant potential, and cholesterol-lowering effects of ethyl acetate fraction derived from Ziziphus mauritiana. Extraction was accomplished using ethyl acetate, and the resultant extract was fractionated through chromatography with a blend of n-hexane and ethyl acetate as …

Butterfly Peacock Bass (Cichla Ocellaris) Neurocranium, Jasmyn Todaro, Emma Horton, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Tradeoffs In Longleaf Pine Conservation: Prescribed Fire Management Increases Exotic Ambrosia Beetle Abundance In Pine-Hardwood Forests, Davide Nardi, Hannah Bares, Natalie A. Clay, Robin M. Verble, Davide Rassati, Lorenzo Marini, John Thomason, John J. Riggins

Biological Sciences Faculty Research & Creative Works

Exotic woodboring insects, including some ambrosia beetles (Coleoptera: Curculionidae, Scolytinae, and Platypodidae), are threatening native forests worldwide. Longleaf pine (Pinus palustris Mill.) forest is an endangered habitat in the southeastern United States. One of the most important actions necessary for its conservation is prescribed fire, which maintains its savanna structure. However, prescribed fire is a forest disturbance, possibly creating new niches for colonization by exotic insects. The interaction between prescribed fire and exotic ambrosia species is understudied, especially in highly fire-dependent ecosystems. We carried out a two-year experiment in mixed longleaf pine forests in Alabama, USA. We compared ambrosia beetle …

Australians Support Multi-Pronged Action To Build Ecosystem Resilience In The Great Barrier Reef, Stewart Lockie, Henry Bartelet, Brent Ritchie, Csilla Demeter, Bruce Taylor, Lintje Sie

Quantitative Methods and Information Technology Faculty Publications

The scale and pace of global environmental change calls for a dramatic upscaling of ecosystem restoration and for actions that build the resilience of ecosystems to future environmental change. This research aimed to quantify public perceptions of threats to the health of the Great Barrier Reef (GBR), Australia, and their support for strategies to address those threats including large-scale restoration and resilience-building actions. We examine how these perceptions change over time and across social cohorts including people living closer to the Reef (n = 2621) and the general Australian population (n = 5825). Respondents were concerned about both …

Srebp Versus Nrf1 Behavior Based On Cholesterol Sensing In Niemann-Pick Type C Using A Cos-7 Model, Christine Tran, Jacob Mcintyre, Felicity Sterling Phd

Science University Research Symposium (SURS)

Niemann-Pick disease type C (NPC) is a rare genetic disorder where cholesterol and lipid transport are blocked leading to accumulation in the lysosomes (National Organization of Rare Diseases (NORD), 2024; Boston Children’s Hospital). This accumulation can lead to neurological issues, such as developmental delay, ataxia, seizures, and early onset of dementia (Patterson, 2000). To study this disease more in depth, scientists have identified a drug called U18666A that induces NPC in the cells it is administered to (Lu, et al., 2015) which allows for the study of other structures and biomolecules in the sterol sensing and transport pathways.

Cholesterol has …

Oceanic Breakthroughs: Marine-Derived Innovations In Vaccination, Therapy, And Immune Health, Chiara Gamberi, Chad L. Leverette, Alexis C. Davis, Moayad Ismail, Ilaria Piccialli, Nicola Borbone, Giorgia Oliviero, Caterina Vicidomini, Rosanna Palumbo, Giovanni N. Roviello

Biology

The vast, untapped potential of the world’s oceans is revealing groundbreaking advancements in human health and vaccination. Microalgae such as Nannochloropsis spp. and Dunaliella salina are emerging as resources for recombinant vaccine development with specific and heterologous genetic tools used to boost production of functional recombinant antigens in Dunaliella salina and Nannochloropsis spp. to induce immunoprotection. In humans, several antigens produced in microalgae have shown potential in combating diseases caused by the human papillomavirus, human immunodeficiency virus, hepatitis B virus, influenza virus, Zika virus, Zaire Ebola virus, Plasmodium falciparum, and Staphylococcus aureus. For animals, microalgae-derived vaccine prototypes have been developed …

What Drives Gut Microbial Diversity And Community Structure Among Closely-Related Host Species?, Rylee Jensen

All NMU Master's Theses

The gut microbiome (GMB) of mammals hosts an important community of microbes that perform countless micro-ecosystem services for the host and are influenced by numerous factors including host physiology, environment, and phylogeny. Phylogeny may have a unique effect on GMB diversity and community structure (i.e., a GMB profile) via phylosymbiosis, a theory in which the evolution of hosts matches the evolution of their associated microbiota. Elucidating phylosymbiosis in mammals is difficult because multiple intrinsic and extrinsic factors collectively drive individual GMB variation. Thus, there is a need to simultaneously analyze the role of physiology, environment, and phylogeny on shaping host …

Assessment Of Heavy Metal Concentration In Orange-Spotted Trevally (Flavocaranx Bajad) From Two Coastal Locations In Abu Dhabi, United Arab Emirates, Kholood Khaled Aljaberi

Theses

This study investigates concentration of heavy metals in the Orange-spotted Trevally (Flavocaranx bajad) from two distinct coastal areas in Abu Dhabi Emirate, Sila and Abu Dhabi. Heavy metals pollution poses a significant risk to both marine ecosystems and human health; thus it is vital to conduct comprehensive assessments of heavy metal contaminations in aquatic life. The main aim of this study is to quantify the levels of heavy metals and assess their concentrations in gill, muscle, and liver tissues of the Orange-spotted Trevally from both sites. The samples were prepared and analyzed using microwave digestion system and ICP …

Role Of B Cell Mediated Immune Response During Skin Atopic Dermatitis Disease, Hidaya Mohammed Abdul Kader

Dissertations

Atopic dermatitis (AD) is the most prevalent non-infectious skin allergy affecting approximately 10-30% adults and 40% children worldwide. It is clinically characterized by eczematic lesions and elevated IgE levels and is a multifactorial disease that is heterogenous in nature. AD results in a dominant Th2 polarized immune response. Until date, there is no cure for this disease. B cells are key players of humoral immune responses and contribute significantly to allergic responses through IgE secretion. However, recently a subset of B cells termed regulatory B cells (Bregs) have been reported to provide protective role by the secretion of anti-inflammatory cytokine …

Pollination By Birds, Non-Flying Mammals, And European Honeybees In A Heathland Shrub, Banksia Catoglypta (Proteaceae), Stanislaw K. Wawrzyczek, Robert A. Davis, Siegfried L. Krauss, Susan E. Hoebee, Louis M. Ashton, Ryan D. Phillips

Research outputs 2022 to 2026

Vertebrate pollination has evolved in many plant lineages, with pollination by birds the most prevalent strategy. However, in Banksia (Proteaceae) many species are visited by a combination of nectarivorous birds, non-flying mammals (NFMs), and insects, though the relative importance of these pollen vectors for reproduction remains poorly understood. Here, we investigate the contribution to fruit set of birds, NFMs, and insects in Banksia catoglypta—a heathland shrub from south-west Australia. This species flowers in winter and produces large, golden-yellow inflorescences that are typically presented near the ground and consist of rigid, strongly scented flowers with a long stigma-nectary distance—suggesting pollination by …

Apoptotic Extracellular Vesicles Carrying Mif Regulate Macrophage Recruitment And Compensatory Proliferation In Neighboring Epithelial Stem Cells During Tissue Maintenance, Safia A Essien, Ivanshi Ahuja, George T Eisenhoffer

Faculty, Staff and Student Publications

Apoptotic cells can signal to neighboring cells to stimulate proliferation and compensate for cell loss to maintain tissue homeostasis. While apoptotic cell-derived extracellular vesicles (AEVs) can transmit instructional cues to mediate communication with neighboring cells, the molecular mechanisms that induce cell division are not well understood. Here, we show that macrophage migration inhibitory factor (Mif)-containing AEVs regulate compensatory proliferation via ERK signaling in epithelial stem cells of larval zebrafish. Time-lapse imaging showed efferocytosis of AEVs from dying epithelial stem cells by healthy neighboring stem cells. Proteomic and ultrastructure analysis of purified AEVs identified Mif localization on the AEV surface. Pharmacological …

Toe Pad Morphology And Adhesion In The Miniaturized Gecko, Chatogekko Amazonicus (Gekkota: Sphaerodactylidae), Aaron H. Griffing, Juan D. Daza, Stuart V. Nielsen, Fernanda P. Werneck, Patrik F. Viana, Tony Gamble

Biological Sciences Faculty Research and Publications

Chatogekko amazonicus is a miniaturized gecko from northern South America and is among the smallest of toe pad bearing lizards. The toe pads of C. amazonicus are miniscule, between 18% and 27% of the plantar surface area. We aimed to investigate the relationship between adhesive toe pad morphology, body size, and adhesive capabilities. Using scanning electron microscopy, we determine that the adhesive pads of C. amazonicus exhibit branched setae similar to those of other geckos, but that are generally much smaller. When compared with other gecko taxa, we show that C. amazonicus setae occupy a similar range of seta length: …

Role Of Male Gonad-Enriched Micrornas In Sperm Production In Caenorhabditis Elegans, Lu Lu, Allison L. Abbott

Biological Sciences Faculty Research and Publications

Germ cell development and gamete production in animals require small RNA pathways. While studies indicate that microRNAs (miRNAs) are necessary for normal sperm production and function, the specific roles for individual miRNAs are largely unknown. Here, we use small RNA sequencing (RNA-seq) of dissected gonads and functional analysis of new loss-of-function alleles to identify functions for miRNAs in the control of fecundity and sperm production in Caenorhabditis elegans males and hermaphrodites. We describe a set of 29 male gonad-enriched miRNAs and identify a set of individual miRNAs (mir-58.1 and mir-235) and a miRNA cluster (mir-4807-4810.1) that …

Structural Molecular And Developmental Evidence For Cell-Type Diversity In Cnidarian Mechanosensory Neurons, Julia Baranyk, Kristen Malir, Miguel A.P. Silva, Sakura Rieck, Gracie Scheve, Nagayasu Nakanishi

Biological Sciences Faculty Publications and Presentations

Deploying a conserved mechanosensory neuron known as the concentric hair cell, cnidarians have evolved diverse mechanoreceptors from hydroid filiform tentacles to jellyfish statocysts. However, it is unknown whether cnidarian mechanoreceptor evolution has relied solely on repurposing a single ancestral mechanosensory neuron type. Here we report evidence for cell-type diversity of mechanosensory neurons in sea-anemone cnidarian Nematostella vectensis. Uncovered in the ectoderm of feeding tentacles are conventional type I hair cells and previously unrecognized type II hair cells differing in the structure of apical sensory apparatus and synapses. Moreover, we identify TRP channel-encoding gene polycystin-1 as a type-II-hair-cell-specific essential mediator of …

Ad Fontes: Divergence-Time Estimation And The Age Of Angiosperms, Stephen A. Smith, Jeremy M. Beaulieu

Biological Sciences Faculty Publications and Presentations

Accurate divergence times are essential for interpreting and understanding the context in which lineages have evolved. Over the past several decades, debates have surrounded the discrepancies between the inferred molecular ages of crown angiosperms, often estimated from the Late Jurassic into the Permian, and the fossil record, placing angiosperms in the Early Cretaceous. That crown angiosperms could have emerged as early as the Permian or even the Triassic would have major implications for the paleoecological context of the origin of one of the most consequential clades in the tree of life. Here, we argue, and demonstrate through simulations, that the …

The Divergent Responses Of Salinity Generalists To Hyposaline Stress Provide Insights Into The Colonisation Of Freshwaters By Diatoms, Kathryn J. Judy, Eveline Pinseel, Kala M. Downey, Jeffrey A. Lewis, Andrew J. Alverson

Biological Sciences Faculty Publications and Presentations

Environmental transitions, such as the salinity divide separating marine and fresh waters, shape biodiversity over both shallow and deep timescales, opening up new niches and creating opportunities for accelerated speciation and adaptive radiation. Understanding the genetics of environmental adaptation is central to understanding how organisms colonise and subsequently diversify in new habitats. We used time-resolved transcriptomics to contrast the hyposalinity stress responses of two diatoms. Skeletonema marinoi has deep marine ancestry but has recently invaded brackish waters. Cyclotella cryptica has deep freshwater ancestry and can withstand a much broader salinity range. Skeletonema marinoi is less adept at mitigating even mild …

Effects Of Retinal Gonadotropin-Releasing Hormone On Reproductive Behavior And Retinal Activity In The Cichlid Fish, Astatotilapia Burtoni, Chase Anselmo

LSU Master's Theses

Many animals use visual signals to evade predators, forage for food, and communicate with conspecifics. Some animals that shift into and out of breeding stages, such as fish, can modulate their visual sensitivity to better adapt to changing internal and external conditions. Previous work in our lab demonstrated that females of the cichlid fish Astatotilapia burtoni have improved visual sensitivity when they enter reproductive readiness. However, little is known about how fish and vertebrates more broadly modulate visual sensitivity across their reproductive cycle. We hypothesized that the hormone and neuromodulator gonadotropin-releasing hormone (GnRH) could modulate visual sensitivity because GnRH is …