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Biochemical and Biomolecular Engineering Commons

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Lithium Recovery Process From Spent Batteries, Nicholas A. Ross, Lacey Roberts, Jordan Leith, Woodi Woodland 2020 University of Tennessee, Knoxville

Lithium Recovery Process From Spent Batteries, Nicholas A. Ross, Lacey Roberts, Jordan Leith, Woodi Woodland

Chancellor’s Honors Program Projects

No abstract provided.


Direct Synthesis Of The Phenanthroviridone Skeleton Using A Highly Regioselective Nitroquinone Diels–Alder Reaction, Huangchao Yu, Ellie Fought, Theresa L. Windus, George A. Kraus 2020 Iowa State University

Direct Synthesis Of The Phenanthroviridone Skeleton Using A Highly Regioselective Nitroquinone Diels–Alder Reaction, Huangchao Yu, Ellie Fought, Theresa L. Windus, George A. Kraus

Chemistry Publications

A variety of nucleophiles react efficiently with in situ generated nitroquinones. The reaction with substituted resorcinols led to the direct synthesis of the phenanthroviridinone and lagumycin skeleton via a highly regioselective Diels–Alder reaction.


Production Of Astaxanthin Using Modified E. Coli Cells, Christina M. Eng, Connor S. Gallagher, Maura K. Kimmel 2020 University of Pennsylvania

Production Of Astaxanthin Using Modified E. Coli Cells, Christina M. Eng, Connor S. Gallagher, Maura K. Kimmel

Senior Design Reports (CBE)

There are several promising markets for naturally synthesized Astaxanthin, a carotenoid found in krill, shrimp, salmon, and other marine life that imparts to these creatures a pink coloring of the flesh and has demonstrated human health-promoting anti-inflammatory and antioxidant activity. Compared to Astaxanthin produced through chemical synthesis, Astaxanthin synthesized through natural fermentative processes (in yeast and algae) is esterified, imparting greater antioxidant activity as well as bioavailability and making it the preferred ingredient for nutraceutical formulations. Additionally, as consumer preferences trend toward natural production processes free from the use of petrochemical solvents which may pose hazards to health hazards, fermentative ...


Production Of Dimethyl Ether (Dme) For Transportation Fuel, Anita S. Yang, Carl V. Antrassian, Julian D. Kurtzman 2020 University of Pennsylvania

Production Of Dimethyl Ether (Dme) For Transportation Fuel, Anita S. Yang, Carl V. Antrassian, Julian D. Kurtzman

Senior Design Reports (CBE)

Dimethyl Ether (DME) is a proposed alternative to diesel fuel that is being looked into by car and truck manufacturers worldwide. The current market, based almost completely in China, is primed for growth and a U.S. based DME total plant that is economical and environmentally feasible stands to pave the way for America’s DME market, especially since states such as California have approved DME for use as vehicle fuel (Fuel Smarts). Conventionally, the DME is produced by feeding Methanol into a xed-bed gas-phase reactor over a ɣ-alumina catalyst (Dimian et al). Using this process and normal operating conditions ...


Rare Earth Element Recovery From Phosphogypsum Using A Biolixiviant, Angelica M. Padilla, Andreea-Maria Pana, Roshan A. Patel 2020 University of Pennsylvania

Rare Earth Element Recovery From Phosphogypsum Using A Biolixiviant, Angelica M. Padilla, Andreea-Maria Pana, Roshan A. Patel

Senior Design Reports (CBE)

This project describes the process of recovering of rare earth elements (REE) from phosphogypsum (PG) waste using a novel biolixviant produced by a bacteria strain known as Glucanobacter oxydans and is inspired by novel research occurring at Idaho National Laboratories. This report details design of a plant which has the capacity to produce the biolixiviant through fermentation, use this biolixiviant to leach REE from a solid waste such as PG, and recover the REE in oxide form through crystallization, filtration, and subsequent high temperature decomposition. The plant has the capability of processing nearly 1 million MT of PG a year ...


Batch Production Of A Potent Small Molecule Active Pharmaceutical Ingredient, Isuru N. Amarasekera, Jinwan Cho, Jason Y. Li 2020 University of Pennsylvania

Batch Production Of A Potent Small Molecule Active Pharmaceutical Ingredient, Isuru N. Amarasekera, Jinwan Cho, Jason Y. Li

Senior Design Reports (CBE)

Small molecule Active Pharmaceutical Ingredients (APIs) have become increasingly relevant in cancer treatment due to their efficacy, targeted treatment, and clinical value. Commonly manufactured in a batch pharmaceutical process, APIs must satisfy Critical Quality Attributes (CQAs) including chemical purity and physical properties. This process involves a multitude of steps, components, and equipment that are optimized to produce an API in a timely and cost-efficient manner. Here, we consider the process and facility design of a batch production of Halfaxia, a new potent anti-cancer drug from Johnson & Johnson. The process begins with a reaction of a starting material and a second ...


Waste Co₂ To Co For Energy Storage, Vignesh C. Bhethanabotla, Joseph C. Dennis, Mavis Athene U Chen 2020 University of Pennsylvania

Waste Co₂ To Co For Energy Storage, Vignesh C. Bhethanabotla, Joseph C. Dennis, Mavis Athene U Chen

Senior Design Reports (CBE)

In this report, we present a process design for the storage of electricity using solid oxide fuel cell (SOFC) technology to convert electrical energy to chemical fuel via the reduction of carbon dioxide. The goal of this project was to design a system that could alleviate the issues around the intermittent nature of renewable energy production, which must phase out the use of fossil fuels in the future. The overall process was developed for two storage strategies, and the economics feasibility of the design is considered and reported. The key aspect of this process is its reversible nature. Both the ...


Commercial Process For The Conversion Of Graphite To Graphite Oxide, Alexander Goodisman, Yash Jain, Dong Bien Kwon 2020 University of Pennsylvania

Commercial Process For The Conversion Of Graphite To Graphite Oxide, Alexander Goodisman, Yash Jain, Dong Bien Kwon

Senior Design Reports (CBE)

With steel and other everyday materials reaching their limits in terms of structural integrity and chemical properties, new materials are needed to provide the next breakthroughs in construction and R&D. Graphene provides incredible strengths and elasticities along with amazing electrical and thermal conductivity. Graphene based materials, the so-called supermaterials, are often limited by the lack of availability due to cost and volume. This report discusses the first large scale production of an important precursor to graphene, graphite oxide (GrO). Based on improvements of Hummer’s method, this project analyses the potential challenges associated with dealing with large quantities of ...


Renewable Ammonia, Kolbein A. Finsnes, James P. Kwon, Dakota M. Wallach 2020 University of Pennsylvania

Renewable Ammonia, Kolbein A. Finsnes, James P. Kwon, Dakota M. Wallach

Senior Design Reports (CBE)

Ammonia is one of the most widely used chemicals that is commercially produced today given the wide need for fertilizer to sustain the world’s ever-growing populations. Given the high world demand for ammonia, which increases every day, one can see how beneficial to the environment that a zero emission large-scale ammonia plant would be. Through the use of energy from Norwegian wind farms, which produce an excess of energy during off-peak hours, our plant design seeks to turn this wasted energy into useful ammonia products at a production rate of 67.2 kmol/hr.

The design of this ammonia ...


Enzyme Production Using An Extremophilic Biocatalyst, Adithya Sriram, Sean M. Begane, Jazriel A. Ortega 2020 University of Pennsylvania

Enzyme Production Using An Extremophilic Biocatalyst, Adithya Sriram, Sean M. Begane, Jazriel A. Ortega

Senior Design Reports (CBE)

Bio-detergents are useful in many markets because they contain enzymes that can break down proteins and in turn enhance stain removal. However, many of these enzymes deactivate at high temperatures so sterilization and stain removal must be done in two separate processes. This process produces extremozymes, enzymes derived from extremophilic microorganisms that have optimal activity and stability at harsh conditions, to be used in bio-detergents. The thermostable characteristics of this enzyme allow it to accomplish high levels of protein breakdown at temperatures conducive to sterilization. It is expected that this characteristic provides a 15% price premium over the leading incumbant ...


Production Of Dl-Methionine From Corn Syrup Via Biosynthesis With Corynebacterium Glutamicum, Dayoung Shin, Yvonne N. Szustakiewicz, Allison P. Walter 2020 University of Pennsylvania

Production Of Dl-Methionine From Corn Syrup Via Biosynthesis With Corynebacterium Glutamicum, Dayoung Shin, Yvonne N. Szustakiewicz, Allison P. Walter

Senior Design Reports (CBE)

DL-methionine is an important feed additive for poultry and swine diets. The amino acid is not produced by animals so it can only be obtained through diet. Methionine is important for protein growth and helps the health of livestock. Recent increase in meat consumption in Latin America and Asia, along with a growing consumer concern of animal welfare, has driven a growth in the methionine market. The methionine market is predicted to continue to grow for at least another five years (Methionine Market 2019). The proposed design is for a process that produces DL-methionine by fermentation with the bacteria, Corynebacterium ...


Design Of Car-T Cell Manufacturing Process, Liam J. Bartie, Lauren R. Duhamel, Ruby C. Pan 2020 University of Pennsylvania

Design Of Car-T Cell Manufacturing Process, Liam J. Bartie, Lauren R. Duhamel, Ruby C. Pan

Senior Design Reports (CBE)

CAR T-cell therapy is at the frontier of personalized immunotherapy. It is a therapy that essentially reprograms a patient’s own T-cells to attack certain blood cancers. A sample of a patient's T cells are collected from the blood, then modified to produce chimeric antigen receptors (CARs) on their surface. When these CAR T cells are reinfused into the patient, the new receptors enable them to latch onto a specific antigen on the patient's tumor cells and kill them, ideally sending the patient into remission and essentially curing their cancer. Currently, CAR T-cell therapy is FDA approved as ...


Mixed Plastics Waste To Ethylene And Propylene Feedstocks, Promise O. Adebayo-Ige, Sarah M. Engelhardt, Matthew C. Larson 2020 University of Pennsylvania

Mixed Plastics Waste To Ethylene And Propylene Feedstocks, Promise O. Adebayo-Ige, Sarah M. Engelhardt, Matthew C. Larson

Senior Design Reports (CBE)

Circular recycle of waste plastic holds significant environmental benefit in reducing the need for crude oil feed to produce plastic monomers and in addressing massive global accumulation of plastic waste. A two-stage cracking process is here explored for the reduction of long-chain polyethylene (PE), polypropylene (PP), and polystyrene (PS) to ethylene and propylene. The reaction yields useful byproducts, such as liquid fuel used to sustain the high energy demands of the process, and pressurized steam. A feed of 70 MT/day of PE, PP, and PS is assumed to be treated first in a rotary kiln pyrolysis reactor and secondly ...


Process Water Recycling, Adrian Barron, Josiah Snider, Kyle Serpe 2020 Olivet Nazarene University

Process Water Recycling, Adrian Barron, Josiah Snider, Kyle Serpe

Scholar Week 2016 - present

The Millipore Sigma-Kankakee Site uses high volumes of water for many operations. One operation utilizes approximately 3600 L of water per batch every couple days and more than 3.8MM liters of water per year. The water is utilized as a component of the buffer washes used in the extraction process for cholesterol that involves various other components such as silica and ions such as sodium, sulfur, and phosphate. The washes that go through the process involve these components and large amounts of water that are then drained out of the facility. This volume is roughly 7% of the site ...


Effective Dispersion Of Mgo Nanostructure On Biochar Support As A Basic Catalyst For Glucose Isomerization, Season S. Chen, Yang Cao, Daniel C.W. Tsang, Jean-Philippe Tessonnier, Jin Shang, Deyi Hou, Zhengtao Shen, Shicheng Zhang, Yong Sik Ok, Kevin C.-W. Wu 2020 The Hong Kong Polytechnic University and National Taiwan University

Effective Dispersion Of Mgo Nanostructure On Biochar Support As A Basic Catalyst For Glucose Isomerization, Season S. Chen, Yang Cao, Daniel C.W. Tsang, Jean-Philippe Tessonnier, Jin Shang, Deyi Hou, Zhengtao Shen, Shicheng Zhang, Yong Sik Ok, Kevin C.-W. Wu

Chemical and Biological Engineering Publications

Glucose isomerization to fructose is one of the most important reactions in the field of biomass valorization. We demonstrate wood waste valorization with MgCl2 salt to synthesize an environment-friendly catalyst (i.e., MgO-biochar), which exhibits effective glucose-to-fructose isomerization with over 30% fructose yield and 80% selectivity at only 100 °C for 30 min in water as a green medium. This study highlights that one-step synthesis can effectively disperse and tether MgO nanostructures to the biochar matrix, which displays a significant reduction of Mg leaching compared to MgO-biochars produced by two-step synthesis and pure MgO. The MgCl2 acts as a porogen ...


Oxidized Phospholipid Regulation Of Chronic Inflammation In Endothelial Cells, Cameron Scott Brutsche 2020 Western Michigan University

Oxidized Phospholipid Regulation Of Chronic Inflammation In Endothelial Cells, Cameron Scott Brutsche

Master's Theses

Atherosclerosis, the primary cause of Coronary Heart Disease (CHD), has become one of the top causes of death in the world. As low-density lipoprotein (LDL) particles become trapped and oxidized inside arterial walls, the primary oxidation products of 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphatidylcholine (PAPC) induce the binding of monocytes which can potentially lead to fatty plaque build ups. A fragment of one of these oxidation products, OxPAPC, has previously been studied and can act as standard for testing new phospholipids and how they affect protein expression levels. The central hypothesis of this study was that three newly studied classes of oxidized fatty ...


Automation Of Cervical Cancer Cytology, Julian Donovan 2020 University of Portland

Automation Of Cervical Cancer Cytology, Julian Donovan

Honors Projects

No abstract provided.


Cancer Immunoimaging With Smart Nanoparticles, Yu-Chuan Ou, Xiaona Wen, Rizia Bardhan 2020 Vanderbilt University

Cancer Immunoimaging With Smart Nanoparticles, Yu-Chuan Ou, Xiaona Wen, Rizia Bardhan

Chemical and Biological Engineering Publications

Dynamic immunoimaging in vivo is crucial in patient-tailored immunotherapies to identify patients who will benefit from immunotherapies, monitor therapeutic efficacy post treatment, and determine alternative strategies for nonresponders. Nanoparticles have played a major role in the immunotherapy landscape. In this review, we summarize recent findings in immunoimaging where smart nanoparticles target, detect, stimulate, and deliver therapeutic dose in vivo. Nanoparticles interfaced with an immunoimaging toolbox enable the use of multiple modalities and achieve depth-resolved whole-body tracking of immunomarkers with high accuracy both before and after treatment. We highlight how functional nanoparticles track T cells, dendritic cells (DCs), tumor-associated macrophages (TAMs ...


High-Throughput Synthesis And Screening Of Rapidly-Degrading Polyanhydride Nanoparticles, Adam S. Mullis, Sarah J. Jacobson, Balaji Narasimhan 2020 Iowa State University

High-Throughput Synthesis And Screening Of Rapidly-Degrading Polyanhydride Nanoparticles, Adam S. Mullis, Sarah J. Jacobson, Balaji Narasimhan

Chemical and Biological Engineering Publications

Combinatorial techniques can accelerate the discovery and development of polymeric nanodelivery devices by pairing high-throughput synthesis with rapid materials characterization. Biodegradable polyanhydrides demonstrate tunable release, high cellular internalization, and dose sparing properties when used as nanodelivery devices. This nanoparticle platform shows promising potential for small molecule drug delivery, but the pace of understanding and rational design of these nanomedicines is limited by the low throughput of conventional characterization. This study reports the use of a high-throughput method to synthesize libraries of a newly synthesized, rapidly-eroding polyanhydride copolymer based on 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) and sebacic acid (SA) monomers ...


A Single Dose Polyanhydride-Based Nanovaccine Against Paratuberculosis Infection, Akanksha Thukral, Kathleen Ross, Chungyi Hansen, Yashdeep Phanse, Balaji Narasimhan, Howard Steinberg, Adel M. Talaat 2020 University of Wisconsin, Madison

A Single Dose Polyanhydride-Based Nanovaccine Against Paratuberculosis Infection, Akanksha Thukral, Kathleen Ross, Chungyi Hansen, Yashdeep Phanse, Balaji Narasimhan, Howard Steinberg, Adel M. Talaat

Chemical and Biological Engineering Publications

Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) causes Johne’s disease in ruminants and is characterized by chronic gastroenteritis leading to heavy economic losses to the dairy industry worldwide. The currently available vaccine (inactivated bacterin in oil base) is not effective in preventing pathogen shedding and is rarely used to control Johne’s disease in dairy herds. To develop a better vaccine that can prevent the spread of Johne’s disease, we utilized polyanhydride nanoparticles (PAN) to encapsulate mycobacterial antigens composed of whole cell lysate (PAN-Lysate) and culture filtrate (PAN-Cf) of M. paratuberculosis. These nanoparticle-based vaccines (i.e., nanovaccines) were well ...


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