Open Access. Powered by Scholars. Published by Universities.®

Biochemical and Biomolecular Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

832 Full-Text Articles 1,692 Authors 912,890 Downloads 69 Institutions

All Articles in Biochemical and Biomolecular Engineering

Faceted Search

832 full-text articles. Page 1 of 35.

Isolation And Production Of Tandem Collagen Binding Domain From Clostridial Collagenase Colg And Developments In C1q Reagent Production For Future Molecule Characterization Work, Stephanie Beitle 2022 University of Arkansas, Fayetteville

Isolation And Production Of Tandem Collagen Binding Domain From Clostridial Collagenase Colg And Developments In C1q Reagent Production For Future Molecule Characterization Work, Stephanie Beitle

Chemical Engineering Undergraduate Honors Theses

This thesis covers a two part project: the production methods to create a double collagen binding domain molecule with a growth factor for wound healing applications and the development of a new in-house production method for isolating C1q from bovine blood. The wound healing molecule was created using transformation, sonication, and purification before being tested via electrophoresis SDS page and Western blots to confirm the molecule’s presence. The C1q in-house production method utilizes an ultrafiltration flow cell rather than dialysis at a critical point in the process, allowing for researchers to not only be able to use a single ...


Drug-Facilitated Sexual Assault At The University Of Arkansas, Barrett Weidman 2022 University of Arkansas, Fayetteville

Drug-Facilitated Sexual Assault At The University Of Arkansas, Barrett Weidman

Chemical Engineering Undergraduate Honors Theses

This work was written to fulfill two main purposes. First, to help survivors of Drug-Facilitated Sexual Assault (DFSA) process their experience by compiling the toxicological, pharmacological, and distribution of the three most used date-rape drugs. Second, to gauge the knowledge and interest of University of Arkansas students regarding drug impairments, sexual assault education, and bystander intervention training. A survey was conducted for the latter and revealed that 91.6% of students believe the University’s existing sexual assault prevention education and bystander intervention training have room for improvement. Also, 37.1% of students who have received this education report that ...


Improving The Biocompatibility Of The Bio-Inorganic Interface For Enhanced Photosystem I-Based Biophotovoltaic Device Performance, Alexandra H. Teodor 2022 University of Tennessee, Knoxville

Improving The Biocompatibility Of The Bio-Inorganic Interface For Enhanced Photosystem I-Based Biophotovoltaic Device Performance, Alexandra H. Teodor

Doctoral Dissertations

The world’s energy demands are projected to increase by nearly 50% by the year 2040, and consumption of carbon-based fuels continues to release greenhouse gases such as carbon dioxide and methane into the atmosphere. This has been causally linked with climate change and increased extreme weather events, which has been further linked to adverse health outcomes and negative effects on biodiversity, food security, and increased disease transmission. Clearly, there is a need for a sustainable, carbon-free, and cost-effective method of energy production to meet growing energy production demands. The sun irradiates Earth’s surface annually with ~80,000 terawatts ...


Crowd Control: Regulating The Spatial Organization Of Biopolymers And Gene Expression By Macromolecular Crowding, Gaurav Chauhan 2022 University of Tennessee, Knoxville

Crowd Control: Regulating The Spatial Organization Of Biopolymers And Gene Expression By Macromolecular Crowding, Gaurav Chauhan

Doctoral Dissertations

The intracellular environment is crowded with macromolecules that can occupy a significant fraction of the cellular volume. This can give rise to attractive depletion interactions that impact the conformations and interactions of biopolymers, as well as their interactions with confining surfaces. We used computer simulations to study the effects of crowding on biologically-inspired models of polymers. We showed that crowding can lead to attractive interactions between two flexible ring polymers, and we further characterized the adsorption of both flexible and semiflexible polymers onto confining surfaces. These results indicate that crowding-induced depletion interactions could play a role in the spatial organization ...


Interactions Between Soft Nanoparticles And Mammalian Cells, Mitchell Raith 2022 University of Tennessee, Knoxville

Interactions Between Soft Nanoparticles And Mammalian Cells, Mitchell Raith

Doctoral Dissertations

Nanoparticles have been of interest to the pharmaceutical industry since the 1980s. The first FDA approved nanoparticle-based therapies included liposomal anesthesia agents. Since then, the amount of FDA-approved nanoparticle therapies remains low. This is because nanoparticle-patient interactions can be very complex and are not well understood. Complicating factors also include increasing obesity rates among the patient population and many small animal pre-clinical trials are completed with healthy, lean animals. The biochemical differences between lean and obese patients prevents early studies from accurately predicting nanoparticle clinical behaviors. Many nanoparticles fail in trails. In this thesis, I aimed to uncover how nanoparticles ...


Reactive Oxygen Species (Ros)-Responsive Size-Reducible Nanoassemblies For Deeper Atherosclerotic Plaque Penetration And Enhanced Macrophage-Targeted Drug Delivery, Jianhua He, Wenli Zhang, Xiaoju Zhou, Fengfei Xu, Jiahui Zou, Qiqi Zhang, Yi Zhao, Hongliang He, Hu Yang, Jianping Liu 2022 Missouri University of Science and Technology

Reactive Oxygen Species (Ros)-Responsive Size-Reducible Nanoassemblies For Deeper Atherosclerotic Plaque Penetration And Enhanced Macrophage-Targeted Drug Delivery, Jianhua He, Wenli Zhang, Xiaoju Zhou, Fengfei Xu, Jiahui Zou, Qiqi Zhang, Yi Zhao, Hongliang He, Hu Yang, Jianping Liu

Chemical and Biochemical Engineering Faculty Research & Creative Works

Nanoparticle-based therapeutics represent potential strategies for treating atherosclerosis; however, the complex plaque microenvironment poses a barrier for nanoparticles to target the dysfunctional cells. Here, we report reactive oxygen species (ROS)-responsive and size-reducible nanoassemblies, formed by multivalent host-guest interactions between β-cyclodextrins (β-CD)-anchored discoidal recombinant high-density lipoprotein (NP3ST) and hyaluronic acid-ferrocene (HA-Fc) conjugates. The HA-Fc/NP3ST nanoassemblies have extended blood circulation time, specifically accumulate in atherosclerotic plaque mediated by the HA receptors CD44 highly expressed in injured endothelium, rapidly disassemble in response to excess ROS in the intimal and release smaller NP3ST, allowing for ...


Model-Guided Design Of Rna-Based Synthetic Circuits For The Dynamic Regulation Of Gene Expression, Jordan R. Ryan 2022 Louisiana State University and Agricultural and Mechanical College

Model-Guided Design Of Rna-Based Synthetic Circuits For The Dynamic Regulation Of Gene Expression, Jordan R. Ryan

LSU Master's Theses

A longstanding goal in synthetic biology has been to build synthetic gene circuits with the ability to harness nature’s capability of precise gene expression regulation. Advancements in RNA technology have established RNA-based regulators with distinct advantages over traditional protein-based regulators such as faster signal propagation, versatile programmability, and low cellular burden, which has created an interest in the field to construct innovative synthetic gene circuits using de novo RNA- based regulators. However, our understanding of the behavior and kinetics of RNA-RNA interactions for the construction of gene circuits is incomplete. This thesis proposes a model-guided design framework that integrates ...


Anaerobic Digestion: Awareness, Implementation, And Simulation, Kieran Tay, Nicholas Sbalbi, Michael Forozis, Flo Sabatini 2022 University of Massachusetts Amherst

Anaerobic Digestion: Awareness, Implementation, And Simulation, Kieran Tay, Nicholas Sbalbi, Michael Forozis, Flo Sabatini

Student Showcase

Anaerobic digestion (AD) is the process in which organic matter is fermented in an oxygen-deprived environment to produce renewable biogas. Our project is multi-faceted, with the overall goal of raising public awareness of AD as a transitional energy source. To that end, our group has named itself Undergraduates Raising Awareness for Anaerobic Digestion (URAAD). We are approaching this goal on three fronts, including (i) a website, (ii) a physical lab-scale digester, and (iii) a computer simulation of anaerobic digestion.

The first component, the website, was developed with the aim of serving as a resource for the public. It includes educational ...


Extraction Of Melanin From Black Knot Fungus: Optimization And Applications, Amara Davis 2022 The University of Akron

Extraction Of Melanin From Black Knot Fungus: Optimization And Applications, Amara Davis

Williams Honors College, Honors Research Projects

The goal of this research project was to optimize the extraction of melanin from black knot fungus and to analyze possible applications of the extracted melanin. An extraction method that was previously created was examined for possible improvements. Improvements that were studied include the elimination of steps in the extraction process while obtaining the same quality of product, examining options for machinery that could be used to increase production rate when moved to a production plant setting, and optimize the concentrations of the raw materials that are used in the extraction for possible reduction of cost without compromising yield or ...


Amino-Polymer For Co2 Capture By Vacuum Swing Adsorption, Sean Billy 2022 The University of Akron

Amino-Polymer For Co2 Capture By Vacuum Swing Adsorption, Sean Billy

Williams Honors College, Honors Research Projects

The applications of sorbent compounds in conjunction with amines and other additives is an innovative method at combating CO2 pollution. An ideal solution would use an amine sorbent to trap the high levels of absorbed CO2 with a low binding energy (less than 45 kJ/mol) and subsequently release them into a stored vessel at ambient temperature with a vacuum swing adsorption system. Attempts at finding a best mixture will be conducted through the usage of a vacuum swing system, utilizing a bed syringe containing the sorbent mix and a vacuum syringe to pull on and subsequently release the CO2 ...


Repurposing Human Α-Defensin 6, An Antimicrobial Peptide, As A Beta Amyloid Inhibitor, Eleanor Plaster 2022 The University of Akron

Repurposing Human Α-Defensin 6, An Antimicrobial Peptide, As A Beta Amyloid Inhibitor, Eleanor Plaster

Williams Honors College, Honors Research Projects

Amyloid aggregation and microbial infection have been identified as the two main pathological risk factors that can result in amyloid diseases such as Alzheimer’s disease (AD), type II diabetes, and Parkinson’s disease. These two risk factors have been well studied separately, however only targeting one of these risk factors has shown to not be successful in preclinical trials since amyloid diseases are multifactorial. In this study, a new approach has been proposed to determine the effects of an antimicrobial and anti-amyloid aggregation approach by looking at the inhibition abilities of a heterocomplex of human α-defensin 6 (HD-6), whose ...


Mimicking The Namib Desert Beetle With Hydrophilic And Hydrophobic Surfaces, Tyler Bracewell 2022 The University of Akron

Mimicking The Namib Desert Beetle With Hydrophilic And Hydrophobic Surfaces, Tyler Bracewell

Williams Honors College, Honors Research Projects

The Namib Desert beetle, Stenocara gracilipes, condenses fog that rolls in off the west coast of Namibia to provide itself with the water it needs for the day. By lifting its abdomen into the air to collect the water vapor, it condenses on hydrophilic bumps that are about 500μm in diameter and are spaced apart about 500-1500μm. Once the water reaches a critical size, the droplet rolls off the hydrophilic bump and onto the flat hydrophobic surface of the elytra. Since the beetle is angled with its abdomen in the air, the water rolls down to its mouth. A design ...


A Solid-State Fermentation Scale-Up Of Scopulariopsis Brevicaulis, Timothy Hudak 2022 The University of Akron

A Solid-State Fermentation Scale-Up Of Scopulariopsis Brevicaulis, Timothy Hudak

Williams Honors College, Honors Research Projects

Publication to be delayed until October 2023


Austenitic, Duplex, And Lean Duplex Stainless Steel Critical Pitting Temperature In Simulated Concrete Environment, Bobby Giebel 2022 The University of Akron

Austenitic, Duplex, And Lean Duplex Stainless Steel Critical Pitting Temperature In Simulated Concrete Environment, Bobby Giebel

Williams Honors College, Honors Research Projects

In this work, the critical pitting temperature (CPT) will be observed for a selection of austenitic (316LN and 24100) and duplex (2205), and lean duplex (2304, 2001) stainless steels in simulated concrete pore solution. To study the influence of temperature on the pitting stability of the stainless steels, three temperatures were tested: 25oC, 45oC, and 65oC for cyclic potentiodynamic polarization (CPP) and electrochemical impedance spectroscopy (EIS). Electrochemical properties of the interfaces we studied via EIS analysis. Kinetics were studied via CPP testing. To reveal the critical pitting temperature of the stainless steels, cyclic thermammetry was used. Characterization of the pits ...


Overcoming Technological Challenges For The Commercialization Of The Circulating Fluidized Bed Bioreactor For Municipal Wastewater Treatment, Michael J. Nelson 2021 The University of Western Ontario

Overcoming Technological Challenges For The Commercialization Of The Circulating Fluidized Bed Bioreactor For Municipal Wastewater Treatment, Michael J. Nelson

Electronic Thesis and Dissertation Repository

The fluidized bed bioreactor as an attached growth wastewater treatment process has demonstrated advantages over suspended growth processes for municipal wastewater treatment applications. However, previous studies have also demonstrated potentially serious disadvantages in terms of energy consumption and maximum reactor size of high flow applications.

In this work, a cost analysis using the CapdetWorks, supplemented by calibrated model data taken from GPS-X was performed to determine the cost effectiveness of the circulating fluidized bed bioreactor (CFBBR). This study demonstrated that the CFBBR is most cost competitive at low flow below 5 MGD. A 10%-20% reduction in net present values ...


Antibody-Drug Nanoparticle Induces Synergistic Treatment Efficacies In Her2 Positive Breast Cancer Cells, Muhammad Raisul Abedin, Kaitlyne Powers, Rachel Aiardo, Dibbya Barua, Sutapa Barua 2021 Missouri University of Science and Technology

Antibody-Drug Nanoparticle Induces Synergistic Treatment Efficacies In Her2 Positive Breast Cancer Cells, Muhammad Raisul Abedin, Kaitlyne Powers, Rachel Aiardo, Dibbya Barua, Sutapa Barua

Chemical and Biochemical Engineering Faculty Research & Creative Works

Chemotherapeutic drugs suffer from non-specific binding, undesired toxicity, and poor blood circulation which contribute to poor therapeutic efficacy. In this study, antibody–drug nanoparticles (ADNs) are engineered by synthesizing pure anti-cancer drug nanorods (NRs) in the core of nanoparticles with a therapeutic monoclonal antibody, Trastuzumab on the surface of NRs for specific targeting and synergistic treatments of human epidermal growth factor receptor 2 (HER2) positive breast cancer cells. ADNs were designed by first synthesizing ~ 95 nm diameter × ~ 500 nm long paclitaxel (PTX) NRs using the nanoprecipitation method. The surface of PTXNRs was functionalized at 2′ OH nucleophilic site using carbonyldiimidazole ...


Towards Understanding Protein Immobilization Rules Through Site-Specific Covalent Immobilization Of T4 Lysozyme, Maxwell Hilbert 2021 Clemson University

Towards Understanding Protein Immobilization Rules Through Site-Specific Covalent Immobilization Of T4 Lysozyme, Maxwell Hilbert

All Dissertations

Soluble enzymes have the unique ability to assist the conversion of complex substrates in fewer steps compared to conventional chemical synthesis; however, when immobilized to a surface, the insoluble enzyme's activity and stability can suffer in its new environment. To improve upon future immobilized enzyme efficacy, a fundamental understanding of the interactions across the enzyme, linker, and surface during covalent, site-specific immobilization that can be detrimental to its activity is required. More specifically, using a well-characterized and model enzyme, T4 lysozyme (T4L), 28 different cysteine attachment points' effects on site-specifically immobilized activity are examined. Different combinations of heterobifunctional amine-to-sulfhydryl ...


Greenhouse Tomatoes: Process Simulation, Juan Gabriel Marin Jr. 2021 University of Arkansas, Fayetteville

Greenhouse Tomatoes: Process Simulation, Juan Gabriel Marin Jr.

Graduate Theses and Dissertations

Growing population demand and challenges brought on by climate change have spurred the need for more resilient fruit and vegetable supply chains. One agricultural technology of significant interest is the use of greenhouses for food production. Greenhouses create a stable and adaptable environment for crops such as tomatoes to grow year-round. Fresh tomatoes are the second most consumed vegetable per capita in U.S. diets, currently averaging 20.7 pounds. The growing consumption of fresh tomatoes has been the result of increasing cultural diversity in the United States.

To meet the growing demand, Venlo-type greenhouses have been frequently used by ...


Continuous Liquid-Liquid Extraction Of Lomustine Synthesis, Devna Grover 2021 Purdue University

Continuous Liquid-Liquid Extraction Of Lomustine Synthesis, Devna Grover

The Journal of Purdue Undergraduate Research

No abstract provided.


Metabolic Modeling Of Cystic Fibrosis Airway Microbiota From Patient Samples, Arsh Vyas 2021 University of Massachusetts Amherst

Metabolic Modeling Of Cystic Fibrosis Airway Microbiota From Patient Samples, Arsh Vyas

Masters Theses

Cystic Fibrosis (CF) is a genetic disorder, found with higher prevalence in the Caucasian population, affecting > 30,000 individuals in the United States and > 70,000 worldwide. Due to the astoundingly high rate of mortality among CF patients being attributed to respiratory failure brought on by chronic bacterial infections and subsequent airway inflammation, there has been a lot of focus on systematically analyzing CF lung airway communities. While it is observed traditionally that Pseudomonas aeruginosa is the most threatening and persistent CF colonizer due to high antibiotic resistance, recent studies have elicited the roles of other pathogens and it has ...


Digital Commons powered by bepress