Biochemical and Biomolecular Engineering Commons^™

C. Difficile Detection Method For First Responder Glove Application, Alli N. Senedak

Williams Honors College, Honors Research Projects

Clostridioides Difficile (C. diff) is a Anaerobic Gram-positive bacillus that is capable of spore formation, making it difficult to control its spread and duration in clinical environments. This phenomenon can provide danger to first responders, healthcare workers, and patients. The goal of this project is to create a biosensor capable of detecting C. diff in a clinical setting that can be applied to a glove apparatus. The project will involve the use of C. diff aptamers activated on the surface of an electrode. Once surface activation has been verified via surface analysis, the electrodes will be exposed to C. diff …

De Novo Protein Binding To Zinc Oxide Through Biomineralization Pathways, Jean-Mark A. Francis

Theses and Dissertations

De novo proteins are structurally distinct from proteins found in nature and thus capable of having their amino acid sequence modified to accomplish tasks such as increasing protein-nanoparticle binding without unfolding or decomposing. Zinc Oxide nanoparticles are functionally distinct from their bulk counterparts and are widely used as semiconductors in a variety of fields such as medicine and agriculture. With demand for these nanoparticles increasing, environmentally sustainable methods of Zinc Oxide nanoparticle synthesis are being investigated as an eco-friendly alternative to currently utilized but environmentally hazardous chemical and physical techniques. This research investigates the binding characteristics between Zinc Oxide nanoparticles …

Age-Related Hearing Loss Involves Mitochondrial Dna Instability And Copy Number Depletion In The Cochlea: Insights From In Vivo And In Vitro Models, Akil Turner, Bo Ding, Xiaoxia Zhu, Tam Nguyen, Parveen Bazard, Robert D. Frisina

Chemical and Biochemical Engineering Faculty Research & Creative Works

Age-related hearing loss (ARHL) is a highly prevalent sensory neurodegenerative disorder that involves various molecular mechanisms. The present study investigated if age-related oxidative stress (OS) induces alterations in mitochondrial DNA (mtDNA) copy number and heteroplasmy, using complementary in vivo and in vitro models. Aged (30-month) CBA/CaJ mice have elevated auditory brainstem response (ABR) thresholds amplitudes vs. young (3-month) CBA/CaJ mice, a key physiological characteristic of ARHL The in vitro experiments employed the strial SV-K1 cochlear cell line treated with hydrogen peroxide (H₂O₂). Both the aged cochleae and H₂O₂-treated cells exhibited a significant …

A Four-Step Thermocatalytic Route For Converting Co2 Into High-Purity Oxalic Acid, Hao Feng, Muhammad Kashif Khan, Lei Li, Hongyan Ma, Xinhua Liang

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

This research outlines a four-step methodology for transforming CO₂ into high-purity oxalic acid. CO2 was first sequestered as KHCO₃ through its reaction with aqueous KOH, followed by hydrogenation using a supported AgPd/CNT catalyst in a batch reactor at an initial H₂ pressure of 60 bar and a temperature of 150°C. The formate yield reached 91.8% at optimum conditions. Potassium formate in the solid state was thermally coupled in the presence of KOH at 400°C under N2 flow, yielding potassium oxalate of up to 76% yield. The final step was the selective precipitation of potassium oxalate with ferrous …

Evaluation Of Chromium-Crosslinked Amps-Hpam Copolymer Gels: Effects Of Key Parameters On Gelation Time And Strength, Maryam Sharifi Paroushi, Baojun Bai, Thomas P. Schuman, Yin Zhang, Mingzhen Wei

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Controlling CO₂ channeling in heterogeneous reservoirs remains a major challenge for both enhanced oil recovery (EOR) and secure geological storage. AMPS-HPAM copolymers exhibit high-temperature resistance and brine tolerance compared with conventional HPAM gels, making them well suited for the harsh environments associated with CO₂ injection. Chromium-based crosslinkers (CrAc and CrCl₃) were investigated because sulfonic acid groups in AMPS can coordinate with trivalent chromium ions, enabling dual ionic crosslinking and the formation of a robust gel network. While organic crosslinked AMPS-HPAM gels have been widely studied, the behavior of chromium-crosslinked AMPS-containing systems, particularly their gelation kinetics under …

Effect Of Ionic Strength On Gelation Time And Strength Of Amps-Based Polymer Gels, Maryam Sharifi Paroushi, Xuyang Tian, Baojun Bai, Thomas P. Schuman, Yin Zhang, Mingzhen Wei

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Polymer gel treatment has been widely applied for improving sweep efficiency and controlling excessive water and gas production. Their performance depends on gelation time and final gel strength. In most studies, brine salinity is used to describe the effect of formation water on gel behavior. However, changing salinity also changes ionic strength and ion composition at the same time. Because of this coupling, it is difficult to identify the mechanisms controlling gelation, which has led to inconsistent trends in the literature. Increasing salinity has been reported to either slow or accelerate gelation and to weaken or strengthen gels depending on …

Solubility And Dissolution Mechanism Of Novel Multi-Ester Headgroup Surfactants In Supercritical Co2, Ning Xu, Yan Ling Wang, Baojun Bai, Shi Zhang Cui, Yu Zhang, Wen Jing Shi, Zhao Nian Zhang, Wen Hui Ding, Pei Xu Ma, Zan Gao

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

To address the limited solubility and applicability of conventional hydrocarbon surfactants in supercritical CO₂, a series of multi-ester headgroup surfactants were designed and synthesized by leveraging the CO₂-philic properties of ester groups. The molecular structures were characterized using Fourier transform infrared (FT-IR) spectroscopy and ¹H NMR. A custom-designed laser-based apparatus was developed to quantify surfactant solubility and systematically investigate phase behavior in CO2. Molecular dynamics (MD) simulations were employed to elucidate structure–solubility relationships across multiple scales, including solubility parameters, interaction energies, radial distribution functions (RDFs), and free volume fractions. Results indicate that, at 323.15 K, …

Evaluation Of A Novel Re-Crosslinkable Preformed Particle Gel For Conformance Control In Ultra-High Temperature Reservoirs, Yanbo Liu, Tao Song, Caleb Kwasi Darko, Thomas P. Schuman, Mingzhen Wei, Baojun Bai

Chemistry Faculty Research & Creative Works

Preferential fluid flow remains a major challenge in subsurface energy production and gas storage operations, resulting in excessive water production in mature oil fields, reduced heat extraction in geothermal reservoirs, and low sweep and storage efficiency in CO₂-EOR projects. Polymer gels are widely used to mitigate high-permeability channels; however, conventional systems exhibit limited plugging efficiency and short lifetimes in ultra-high-temperature reservoirs due to poor thermal stability. This study presents a novel ultra-high-temperature-resistant preformed particle gel (UHT-PPG) developed for conformance control in reservoirs with temperatures of 150–275 °C and severe super-K or channeling problems. The material was evaluated in …

Calibration And Validation Of Cyclic Stretching Platform For Stiffness Testing Of Engineered Heart Tissue, Jillian Welsh

Chemical Engineering Undergraduate Honors Theses

Cardiac fibrosis (CF) is a major contributor to the high mortality rate of heart failure which affects over 23 million people worldwide. CF involves cardiac fibroblasts depositing extracellular matrix, made primarily of collagen type I, which stiffens the heart walls over time. As testing new drugs for CF is deterred by the high cost and time required for animal and clinical trials, this research helps to solve the problem by providing an in vitro platform to study fibrosis.

The work involved creating tissues containing human cardiac fibroblast (HCFB) cells. Spring testing was conducted to determine the force applied by the …

Purifying Circular Rna Therapeutics, Karen Guillen-Cuevas

All Dissertations

Circular RNA (circRNA) has emerged as a promising therapeutic modality due to its enhanced stability and resistance to exonuclease degradation compared with linear mRNA. However, its stability and functionality depend strongly on achieving high purity, and current methods such as size-exclusion chromatography suffer from peak overlap, low yields, and poor scalability. This dissertation addresses these limitations by developing ultrafiltration-based strategies to purify circRNA directly from in vitro transcription (IVT) and self-splicing (circularization) reactions. First, the feasibility of ultrafiltration was demonstrated using polyethersulfone membranes, where sieving coefficients and critical flux values of circRNA, precursor RNA, and nicked RNA were measured to …

Bone Void Filler Materials For Augmentation In Comminuted Fractures: A Comprehensive Review, S. Sina Mohammadi, Sunjeev S. Phull, B. Sonny Bal, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

Comminuted fractures, characterized by multiple bone fragments, present significant challenges in orthopedic surgery. Effective treatment often requires augmentation techniques to enhance fixation stability and promote bone regeneration. This review explores the application of bone void filler materials, including autografts, allografts, polymethyl methacrylate (PMMA), and synthetic bone substitutes such as calcium phosphate ceramics and bioactive glass, in managing comminuted fractures. Autografts are the gold standard due to their superior osteogenic potential but are limited by donor site morbidity and availability. Allografts mitigate these issues but face concerns regarding immunogenicity and reduced biological activity. PMMA, widely used for structural augmentation, provides immediate …

Fluorosilane-Induced Softening And Collapse Of Micropillar Arrays, Gabriela Moreira Lana, Maja Fehlberg, Petra Herbeck-Engel, Gisela Heppe, Raimund Schlüßler, Torsten Jähnke, Eduard Arzt, Roland Bennewitz

Chemical and Biochemical Engineering Faculty Research & Creative Works

Replica molding is a widely used technique for the fabrication of polymer microstructures. As structural dimensions decrease, anti-stick surface treatment of the mold becomes increasingly critical to ensure clean demolding and preserve structural integrity. We fabricated arrays of micropillars with 20 µm diameter and 60 µm height using medical-grade polydimethylsiloxane (PDMS), MDX4-4210, and observed a high fraction of collapsed pillars for the first molding after fluorosilanization of the mold to reduce sticking. To address this issue, we systematically investigated the surface treatment protocol for the molds, made from the PDMS Sylgard 184. We provide results from complementary measurement methods, to …

Synergistic Doping And Coating Strategy For Boosting Na0.7mno2 Cathode Performance In Sodium-Ion Batteries, Ahmad Helaley, Han Yu, Manashi Nath, Xinhua Liang

Chemistry Faculty Research & Creative Works

Advancing sodium-ion battery (SIB) technology requires novel approaches to optimize cathode materials for improved electrochemical performance. In this study, we employ atomic layer deposition (ALD) to precisely modify the surface of P2-type Na_0.7MnO₂ cathodes with different coating materials including ZnO, NiO, and Al₂O₃, followed by post-annealing at 750°C for 10 hours. The strategic combination of ALD and thermal treatment can achieve thin film coating on particle surface and promote element doping into the near-surface lattice, as confirmed by electron energy loss spectroscopy (EELS) analysis. The incorporation of Zn, Al, and Ni results in …

A Comprehensive Overview Of Chronic Wound Infections And Current Treatment Methods, Sarah Fakher, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

Chronic wound infections pose significant healthcare challenges due to persistent biofilms, antibiotic resistance, and impaired healing pathways. These wounds are characterized by prolonged inflammation, microbial colonization, and disrupted tissue regeneration, leading to substantial morbidity and healthcare costs. This article reviews current knowledge on chronic wound types, their pathophysiology, and the mechanisms underlying biofilm formation to examine the latest advancements in antimicrobial-based strategies aimed at addressing these challenges. It highlights how diverse materials and technologies have been engineered to improve infection management, enhance tissue regeneration, and overcome the limitations of traditional treatments as well as advances that leverage innovations such as …

Few-Layered Conductive Graphene Foams For Electrical Transdifferentiation Of Mesenchymal Stem Cells Into Schwann Cell-Like Phenotypes, Ekin G. Simsar, Peifu Cheng, Tugce Dogruel, Maxsam Donta, Juhyung Jung, Naomi A. Asante, Donald S. Sakaguchi, Surya K. Mallapragada, Piran R. Kidambi, Metin Uz

Chemical & Biomedical Engineering Faculty Publications

This study investigates the potential of few-layered conductive graphene foams as 3D platforms for the electrical transdifferentiation of mesenchymal stem cells (MSCs) into Schwann cell (SC)-like phenotypes for peripheral nerve injury (PNI) treatment. The 3D graphene foams (3D-GF) are cytocompatible with MSCs and created a favorable microenvironment for the cells to attach, grow, proliferate, and transdifferentiate. We demonstrated that MSCs cultured within 3D-GF can be transdifferentiated into SC-like phenotypes using the synergistic effects of electrical stimulation and 3D porous and conductive structure. Our immunocytochemistry and gene expression analyses showed the expression of Schwann cell markers and enhanced secretion of growth …

Condition Optimization For The Synthesis Of Castor Oil Templated Mesoporous Silica, Godlisten Namwel Shao

Tanzania Journal of Engineering and Technology (TJET)

The present work provides suitable conditions for the synthesis of mesoporous materials with improved porosity using an optimization technique. The proposed preparation method involves forming final products by varying the amount of castor oil, media, and template removal. The obtained samples were examined by TGA, XRD, DRIFT and nitrogen physisorption studies. It was observed that the porosity of the obtained samples was dependent on the conditions under which the materials were synthesized. All synthesized materials showed the Type IV adsorption-desorption isotherm features of mesoporous, regardless of the conditions utilized during the synthesis. The sample synthesized using 2.5 g of a …

Laboratory Investigation Of High-Temperature Preformed Particle Gels For Fluid Control In Granite Cores For Geothermal Applications, K. Caleb Darko, Yanbo Liu, Thomas P. Schuman, Mingzhen Wei, Baojun Bai

Chemistry Faculty Research & Creative Works

To understand the applicability of high-temperature preformed particle gel (HT-PPG) for control of short-circuiting in enhanced geothermal systems (EGSs), core flooding experiments were conducted on fractured granite cores under varying fracture widths, gel particle sizes and swelling ratios. Key parameters such as injection pressure, water breakthrough pressure, and residual resistance factor were measured to evaluate HT-PPG performance. The gel exhibited strong injectability, entering granite fractures at pressure gradients as low as 0.656 MPa/m; HT-PPG yields a superior sealing performance by significantly reducing the permeability; and dehydration occurs during HT-PPG propagation, with a dehydration ratio ranging from 4.71% to 11.36%. This …

Effect Of Clays On The Spontaneous Imbibition Behavior Of Surfactant Solutions In Shale Oil Reservoirs, Ning Xu, Yanling Wang, Baojun Bai, Chuanbao Zhang, Shizhang Cui, Yu Zhang, Di Li, Wenjing Shi, Wenhui Ding

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Surfactant-enhanced spontaneous imbibition is a proven method of enhancing oil recovery from shale reservoirs. However, a significant knowledge gap concerning the impact of clay minerals on surfactant-enhanced imbibition in shale reservoirs remains. Therefore, this study first analyzed the mineral composition and pore structure of the shale reservoirs. Subsequently, three surfactants were selected for spontaneous imbibition experiments. The influence of various clay minerals on the surfactant imbibition was assessed by considering various properties of the imbibition system. The results showed that the shale pores primarily consisted of macropores (44.03%) and mesopores (43.94%). Surfactants enhance the imbibition recovery by altering the wettability …

Coumarin/Nitrogen-Bearing Heterocyclic Hybridloaded Electrospun Pmma/Pvp Nanofibrous Scaffolds For Accelerating Topical Wound Healing Rates: Synthesis And In Vitro Bio-Evaluation, Samar A. Salim, Mohamed Ali, Tasneem Abed, El Badawy Kamoun

Nanotechnology Research Centre

Coumarin-nitrogen heterocyclic compounds (e.g. quinoline, acridine, and phthalazine) were synthesized by facile reactions and elucidated by FTIR, 1H, and 13C-NMR analyses, which showed consistency with the expected structures. Coumarin-quinoline (drug A)- and coumarin-acridine (drug B)-loaded electrospun PMMA/PVP nanofibrous scaffolds were fabricated using electrospinning techniques. Results showed a successful PMMA/PVP blend, which formed the matrix that was used as the main scaffold for drug loading/release. The drugs (A and B) were encapsulated within the matrix as verified by IR and SEM results. Bio-evaluation through cytotoxicity and anticancer screening was conducted using the MTT assay against lung fibroblast (Wi-38), colon carcinoma (Caco-2), …

Upcycling Waste To Wealth: Cuo-Sio₂/Reduced Graphene Nanocomposite From Pomegranate Peels For One-Pot Low-Temperature Conversion Of Waste Oils Into Valuable Fatty Acid Monomers, Attia Attia, Shahenda Mahran Dr., Maria Centeno Prof., Basudeb Saha

Petroleum Engineering and Gas

and hence reduce the price of the fatty acid monomers. This study reports for the first time a novel, environmentally benign, highly active copper oxide-silica oxide/reduced graphene oxide (CuO-SiO2/RGO), heterogeneous nano-catalyst derived from waste pomegranate peels, for the one-pot, low-temperature synthesis of fatty acid monomers from high-acid-value waste vegetable oil (WVO). The synthesised nano-catalyst was extensively characterised using XRD, FT-IR, TEM, SEM, EDX and TGA-DTA. Further, it was utilised to synthesise fatty acidrich oleic phenoxypropyl acrylate (OPA) monomer from high acid value WVO via a single-step reaction.