Biochemical and Biomolecular Engineering Commons^™

Materials Education And Research In Art And Design: A New Role For Libraries (Program Sheet), Mark Pompelia

Materials Education and Research in Art and Design: A New Role for Libraries

No abstract provided.

Materials Education And Research In Art And Design: A New Role For Libraries (Program Booklet), Mark Pompelia

Materials Education and Research in Art and Design: A New Role for Libraries

No abstract provided.

Materials Education And Research In Art And Design: A New Role For Libraries (Website), Mark Pompelia

Materials Education and Research in Art and Design: A New Role for Libraries

No abstract provided.

Materials Collection Creation And Administration: A New Role For Libraries (White Paper), Mark Pompelia

Materials Education and Research in Art and Design: A New Role for Libraries

The Problem/Opportunity: To define, identify, and guide design-based materials collections in academic settings and foster community among those with existing collections and/or those considering creating and supporting one.

Contents and topics:

1. What is a materials collection?
2. Why have a materials collection?
3. Acquisition strategies
4. Organizational approaches
5. Programming possibilities
6. Symposium summary
7. Resources

Characterisation Of Ga2o3-Na2o-Cao-Zno- Sio2 Bioactive Glasses, A. W. Wren, T. Keenan, A. Coughlan, F. R. Laffir, D. Boyd, Mark R. Towler, M. M. Hall

Chemical and Biochemical Engineering Faculty Research & Creative Works

The structural role of Gallium (Ga) is investigated when substituted for Zinc (Zn) in a 0.42SiO₂-0.40-xZnO-0.10Na₂O-0.08CaO glass series, (where x = 0.08). Each starting material was amorphous, and the network connectivity (NC) was calculated assuming Ga acts as both a network modifier (1.23), and also as a network former. Assuming a network forming role for Ga the NC increased with increasing Ga concentration throughout the glass series (Control 1.23, TGa-1 2.32 and TGa-2 3.00). X-ray photoelectron spectroscopy confirmed both composition and correlated NC predictions. Raman spectroscopy was employed to investigate Q-structure and found that a shift …

Increasing Round Trip Efficiency Of Hybrid Li-Air Battery With Bifunctional Catalysts, Kan Huang, Yunfeng Li, Yangchuan Xing

Chemical and Biochemical Engineering Faculty Research & Creative Works

Previously it was shown that Pt as cathode catalyst has a large overpotential during charge in rechargeable hybrid Li-air battery with sulfuric acid catholyte. This article demonstrates that a bifunctional catalyst composed of Pt and IrO2 supported on carbon nanotubes can address this problem. The specially designed and synthesized bifunctional catalyst showed significant overpotential reduction and achieved a round-trip energy efficiency of 81% after 10 cycles, higher than many achieved in aprotic Li-O2 batteries. The hybrid Li-air battery was discharged and recharged for 20 cycles at 0.2 mA/cm2, showing a fairly stable cell performance. A specific capacity of 306 mAh/g …

In Vivo Efficacy Of Chitosan/Interleukin-12 In Controlling Lung Metastasis In 4t1 Breast Cancer Model, Jimmy Vo

Biomedical Engineering Undergraduate Honors Theses

Cancer immunotherapy has emerged as a leading front in cancer treatment. In contrast to other treatment methods such as radiation and surgery, immunotherapy trains the patient's body to recognize and eliminate tumors, thus preventing reoccurrence of secondary tumors. IL-12 has been shown to display a potent anti-tumor immune response in mice; however, when clinical trials were conducted, it was shown to be toxic and in some cases fatal when administered systemically.1,2 Because of this, local delivery of IL-12 is under investigation. Our lab has shown that when paired with the polymer chitosan, local, intratumoral (i.t.) injections of IL-12 are retained …

Highly Sensitive Method For Detecting And Separating Pathogens Using Paramagnetic Particles And A Micro-Fluidic System, William Ryan

Biomedical Engineering Undergraduate Honors Theses

Water is one of the most important compounds on Earth. If a water supply is contaminated with a pathogen, it can have devastating results to an individual or a community. This research is directed at constructing an easily reproducible procedure that could increase the sensitivity of detecting and separating bacteria from the current limits of about 100 cells to 10-20 cells per sample with a ultimate goal of a single cell detection. Here, we report a micro-fluidic system to magnetically capture pathogenic bacteria in water followed by rapid impedance detection using Escherichia coli K-12 as a model bacterium. Paramagnetic beads …

Comparing Decellularized Muscle Tissue With An Engineered Muscle Biomaterial, Katelin Cherry

Biomedical Engineering Undergraduate Honors Theses

Decellularized tissues composed of extracellular matrix are commonly used for tissue transplantations but this method has many limitations. An alternative approach is to create an extracellular matrix by seeding degradable foams with cells, culturing these foams to allow extracellular matrix to be secreted from the cells, then washing away the degradable foam to collect the material that is produced. This study was interested in comparing decellularized skeletal muscle tissue to an engineered biomaterial that was seeded with skeletal muscle myoblast cells. Collagen content of decellularized tissue and engineered biomaterial were quantified using a hydroxyproline assay to compare the two samples. …

Rapid Detection And Quantification Of Mycobacterium Tuberculosis Using Single-Based Extension And Capillary Electrophoresis, Anh Vu

Biomedical Engineering Undergraduate Honors Theses

In 1993, the World Health Organization (WHO) has declared tuberculosis (TB) a global emergency. Since then, more than 30 million lives have been claimed by that world-wide epidemic. In 2011, 8.7 million people “felt ill” because of TB and not all TB cases were reported to clinicians. Therefore, it is important to accurately identify TB patients by developing a diagnostic method that is sensitive, fast, and cost-effective. However, conventional methods have not met those criteria because they either require lengthy procedures or may misdiagnose TB cases. Hence, the automated Xpert MTB/RIF, endorsed by WHO in 2010, was developed and so …

Expression, Production, And Purification Of Novel Therapeutic Proteins, Mckinzie Shea Fruchtl

Graduate Theses and Dissertations

Interest in the production of recombinant proteins consisting of collagen binding domain (CBD) fused to a bioactive material has increased due to the targeting/attachment capabilities of CBD. For example, CBD fusions can be applied to the reversing of bone density loss and the repair of the eardrum, specifically, by choosing an appropriate fusion partner (parathyroid hormone or epidermal growth factor). The production of CBD fusions was examined using batch and fed-batch culturing of Escherichia coli to express the fusion proteins, and affinity chromatography to isolate the final product.

Different medium formulations, feeding strategies, and induction methods were tested in order …

Bio-Separation Process Improvement Via Genomic Manipulation: Development Of Novel Strains For Use In Immobilized Metal Affinity Chromatography (Imac), Ryan Curtis Haley

Graduate Theses and Dissertations

The dissertation is comprised of three parts. Part I describes proteomic analysis of native bacterial proteins from Escherichia coli (E.coli) that bind during Immobilized Metal Affinity Chromatography (IMAC). Part II describes the value in exploiting proteome based data as a tool toward the design an E. coli expression strain that is particularly useful when Immobilized Metal Affinity Chromatography is employed as the initial capture step of a homologous protein purification process. Part III describes a methodology of chromosomal mapping of all contaminant gene products.

The objective of Part I was to identify all E. coli proteins that bind to Co(II), …

Aluminium-Free Glass Polyalkenoate Cements: Ion Release And In Vitro Antibacterial Efficacy, A. W. Wren, J. P. Hansen, S. Hayakawa, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

Glass polyalkenoate cements (GPCs) have exhibited potential as bone cements. This study investigates the effect of substituting TiO₂ for SiO₂ in the glass phase and the subsequent effect on cement rheology, mechanical properties, ion release and antibacterial properties. Glass characterization revealed a reduction in glass transition temperature (T_g) from 685 to 669 C with the addition of 6 mol % TiO₂ (AT-2). Magic angle spinning nuclear magnetic resonance (MAS-NMR) revealed a shift from -81 ppm to -76pmm when comparing a Control glass to AT-2, indicating de-polymerization of the Si network. The incorporation of TiO_{2 …}

Sensor Application Of Poly (Ethylene Glycol) Diacrylate Hydrogels Chemically-Anchored On Polymer Surface, Zhan Gao, Chang-Soo Kim, David B. Henthorn

Electrical and Computer Engineering Faculty Research & Creative Works

We demonstrate a device process combining two photo patternable polymers, poly (ethylene glycol) (PEG)-diacrylate-based-hydrogel and epoxy-based photoresist SU-8, to implement an optofluidic bioanalytical platform through a surface anchoring technique. As an exemplary sensor application, optical dissolved oxygen sensors are fabricated and their performance characterized. The PEG-rich hydrogel is used as a matrix material for the immobilization of oxygen-responsive fluorophore, dichlorotris (1, 10-phenanthroline) ruthenium (II) hydrate. This hydrogel is chemically anchored on the surface of negative-tone photoresist, SU-8, through a free radical reaction in which 1-hydroxycyclohexyl phenyl ketone served as the surface bound photo initiator. The sensor exhibits a reversible Stern-Volmer …

Method Of Purifying Crude Noscapine, Benjamin M. Marshall, Keith Tomazi

Chemical and Biochemical Engineering Faculty Research & Creative Works

The invention provides a method for separating noscapine from an opium source without substantially changing the color of the noscapine.

Carbothermal Synthesis Of Titanium Oxycarbide As Electrocatalyst Support With High Oxygen Evolution Reaction Activity, Kan Huang, Yunfeng Li, Yangchuan Xing

Chemical and Biochemical Engineering Faculty Research & Creative Works

Carbothermal reduction of semiconducting TiO₂ into highly conductive titanium oxycarbide (TiOxCy) was investigated. The thermally produced uniform carbon layer on TiO₂ (Degussa P25) protects the TiO₂ nanoparticles from sintering and, at the same time, supplies the carbon source for doping TiO₂ with carbon. At low temperatures (e.g., 700 °C), carbon only substitutes part of the oxide and distorts the TiO₂ lattice to form TiO₂-xCx with only substitutional carbon. When the carbon-doped TiO₂ is annealed at a higher temperature (1100 °C), x-ray diffraction and x-ray photoelectron spectroscopy results showed that TiOxCy, a solid …

Picture Of A Chelate In Exchange: The Crystal Structure Of Nahodotma, A 'Semi'-Hydrated Chelate, Katherine M. Payne, Edward J. Valente, Silvio Aime, Mauro Botta, Mark Woods

Chemistry Faculty Publications and Presentations

Crystallography generally only provides static structural information. This can render it an ineffective technique for probing dynamic solution state processes. A crystal of HoDOTMA affords unique structures that effectively represent that of a lanthanide tetra-acetate chelate mid-way through the water exchange process.

Numerical Evaluation Of The Stability Of Stationary Points Of Index-2 Differential-Algebraic Equations: Applications To Reactive Flash And Reactive Distillation Systems, D. A. Harney, T. K. Mills, N. L. Book

Chemical and Biochemical Engineering Faculty Research & Creative Works

The dynamic behavior of many chemical processes can be represented by an index-2 system of differential-algebraic equations. This index can be reduced by differentiation, but unfortunately the index reduced systems are not guaranteed to possess the same stability characteristics as that of the original system. When the set of differential-algebraic equations can be written in Hessenberg form, the matrix pencil of the linearized system can be used to directly evaluate the stability of a steady state without the need for index reduction. Direct evaluations of stability of reactive flash and reactive distillation are presented. It is also shown that a …

A New Method For Online Flow Regime Monitoring In Bubble Column Reactors Via Nuclear Gauge Densitometry, Ashfaq Shaikh, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

In the Current Work, Nuclear Gauge Densitometry (NGD), Which is Commercially Available and Industrially Used for Liquid/slurry Level Measurement and Control, Has Been Employed to Identify Fingerprints of the Prevailing Hydrodynamic Flow Regime. Experiments Were Performed using an Air-Water System in 0.1012. M Diameter and 1.2. M Long Bubble Column at Ambient Pressure. the Superficial Gas Velocities Were Varied from 1. Cm/s to 12. Cm/s with an Interval of 0.5. Cm/s Near Transition Region. the Flow Regime Boundaries Were Defined using the Conventional Methods of Flow Regime Demarcation Such as the Change in the Slope of the overall Gas Holdup …

Comparison Of A Sio2-Cao-Zno-Sro Glass Polyalkenoate Cement To Commercial Dental Materials: Glass Structure And Physical Properties, A. W. Wren, A. Coughlan, F. R. Laffir, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

Glass polyalkenoate cements (GPCs) have previously been considered for orthopedic applications. A Zn-GPC (BT 101) was compared to commercial GPCs (Fuji IX and Ketac Molar) which have a setting chemistry analogous to BT 101. Handling properties (working, T_w and setting, T_s times) for BT 101 were shorter than the commercial GPCs. BT 101 also had a higher setting exotherm (S_x - 34 °C) than the commercial GPCs (29 °C). The maximum strengths for BT 101, Fuji IX, and Ketac Molar were 75, 238, and 216 MPa (compressive, σ_c), and 34, 54, and 62 MPa (biaxial …