Pharmacology, Toxicology and Environmental Health Commons^™

Identification And Antimicrobial Activities Of Actinomycètes From Soils In Samed Island, And Geldanamycin From Strain Pc4-3, Wijittra Kittivachra, Somboon Tanasupawat, Surattana Amnuoypol, Khanit Suwanborirux

The Thai Journal of Pharmaceutical Sciences

In the course of identification and screening for antimicrobial activities of 100 actinomycète isolates from 35 soil and sand samples collected from the shore of Samed Island, Rayong province, 79 isolates were identified as Streptomyces, 20 were Micromo...

In Vitro Toxicity Assessment Of Silver Nanoparticles In Rat Alveolar Macrophages, Cataleya Carlson

Browse all Theses and Dissertations

The present study was conducted to assess the toxicity of nanosized silver particles (Ag-15nm, 30nm and 55nm) in rat alveolar macrophages. For toxicity evaluations, cellular morphology, mitochondrial function (MTT assay), membrane leakage (LDH assay) and reactive oxygen species generation (ROS) were assessed after a 24h under control and exposed conditions. The morphological appearance of control and exposed cells were observed by an inverted, phase contrast microscope and the uptake of nanoparticles was observed using the CytoViva Ultra Resolution Imaging (URI) system. Morphological images captured at 100x magnification demonstrated that nanoparticle-exposed cells at higher doses became abnormal in size, and most …

Kinetics And Passive Protection Efficacy Induced By Purified Ava Human Immunoglobulin G In Rabbits Against A Bacillus Anthracis Aerosol Challenge, Jennifer Lee Plahovinsak

Browse all Theses and Dissertations

The present study was conducted to determine the half-life, assess the toxicity, and passive protection efficacy of purified immunoglobulin G (IgG) from Anthrax Vaccine Adsorbed (AVA) vaccinated human donors. Half-life determinations were calculated from the reportable values obtained using the anti-PA ELISA assay and the Centers for Disease Control's (CDC) "ELISA for Windows" software. For toxicity evaluations animals were observed clinical for one hour post administration and for 14-days post-treatment. The protection efficacy was determined based upon the mortality results from a lethal Bacillus anthracis aerosol challenge. While no protection was achieved in this delayed exposure scenario, the study yielded …

Spectrophotometric Determination Of Binary Mixtures Of Prednisolone With Some Antibiotics, Abd El-Maboud I. Mohamed, Hesham Salem, Eman Maher

The Thai Journal of Pharmaceutical Sciences

Multivariate (classical least squares and principal components regression) techniques and derivative spectrophotometric techniques (first derivative and derivative ratio) were developed for the assay of two binary mixtures of prednisolone with tetracycli...

A Switch From Polyandry To Serial Monogamy: Results From A Three Year Tagging Study Of Horseshoe Crabs In Long Island Sound, Jennifer Mattei Ph.D., Paul Goodell, Christine Depierro, Meghann Burke

Biology Faculty Publications

Part of an ongoing population study of the North American Horseshoe Crab, Limulus polyphemus, in Long Island Sound was completed during the 2003 – 2005 spawning seasons at Milford Point, in Milford, CT. Horseshoe crabs range more widely throughout Long Island Sound than expected and exhibit weak site fidelity. Animals originally tagged in Milford were found as far west as Stamford and as far east as Clinton, CT. during the 3 year study. Out of 522 female horseshoe crabs tagged in 2004 only 4 came back to spawn again at Milford Pt. in 2005. The sex ratios of tagged and …

The Presence, Prevalence And Antibiotic Resistance Of Microbes In The Pamunkey River Basin, Christina Mcdowell

Honors Theses

This project examined the presence, prevalence and antibiotic resistance of Coliforms (mainly Escherichia coli), Staph/occocus aureus, fecal Streptococcus, andEnterobacteriacae found in the water samples taken from three selected sites on the North Anna, South Anna, and Little Rivers of the Pamunkey River Basin. The diversity and concentration of the microbes isolated from these sites was evaluated in late January and late March allowing for a comparison of the occurrence of microbes at different times and between the different sites. Over the course of two months the concentration of colonies increased based on colony counts from Nutrient agar. The rate of …

The Involvement Of Calcium And Map Kinase Signaling Pathways In The Production Of Radiation-Induced Bystander Effects, Fiona Lyng, Paula Maguire, B. Mcclean, C. Seymour, Carmel Mothersill

Articles

Much evidence now exists regarding radiation-induced bystander effects, but the mechanisms involved in the transduction of the signal are still unclear. The mitogen-activated protein kinase (MAPK) pathways have been linked to growth factor-mediated regulation of cellular events such as proliferation, senescence, differentiation and apoptosis. Activation of multiple MAPK pathways such as the ERK, JNK and p38 pathways have been shown to occur after exposure of cells to radiation and a variety of other toxic stresses. Previous studies have shown oxidative stress and calcium signaling to be important in radiation-induced bystander effects. The aim of the present study was to investigate …

The Effects Of The Herbicide Atrazine On Human Liver Cells, Erin R. Powell

Honors Program Theses

Atrazine is one of the most commonly used herbicides in the United States, in particular for maize crops. This herbicide is very effective against weeds. However, it has also been associated with many harmful health effects in non-target organisms such as amphibians and mammals. One of the non-target effects of atrazine may be to act as an endocrine disruptor in different cells. To study the endocrine system effects of atrazine in human cells, a human liver cell line (HepG2) was used. In this study, we determined the effects on growth of HepG2 cells after exposure to increasing concentrations of this …

Development Of Single Nanoparticle Optical Assays For Imaging Single Living Cells, William John Brownlow

Chemistry & Biochemistry Theses & Dissertations

Multi-drug resistance (MDR) has been reported in both prokaryotes and eukaryotes; the pathogenic gram-negative bacteria Pseudomonas aeruginosa can extrude a variety of structurally and functionally diverse substrates via a number of membrane transport systems leading to MDR. We have developed a novel nanoparticle assay to characterize both the membrane transport system composed of the MexAB-OprM efflux pump and the membrane permeability induced by antibiotics. Gold (Au) and silver (Ag) nanoparticles were investigated for use as probes to explore membrane transport in P. aeruginosa.

The surface plasmon absorption (color) of Au nanoparticle solutions was found to change in the presence …

Chemical Constitutents And Cytotoxicity Of Some Tanzanian Wild Mushrooms, V. Nyigo, L. D. Baraza, M. H. H. Nkunya, S. J. M. Mdachi, C. C. Joseph, A. Waziri

Tanzania Journal of Science

The sterol ergosterol and ergosta-4,22-diene-3β,7α-diol, together with 4-hydroxybenzaldehyde were isolated from the mushroom species Polyporus molluscensis, Cantharellus isabelinus, C. symoensii and a Podaxis species. Their structures were established on the basis of spectroscopic data. The ethanol extracts of mushroom samples of Agaricus sp. and Termitomyces letestui also showed cytotoxicity against the brine shrimp larvae.

La Flore Qui A Faim : Une Enquête Chimique, Biologique Et Culturelle De La Dimorphisme Rouge/Verte Et Les Applications Médicinales De Nepenthes Madagascariensis, Keirnan Willett

Independent Study Project (ISP) Collection

Le Nepenthes madagascariensis est une plante carnivore endémique du côté sud-est de Madagascar qui attirent la proie arthropode (insectes, araignées etc.) en employant les bouts de feuilles convertis en forme des pichets ou urnes. Après d’être attiré par le suc au bord de l’urne, les insectes devint désorientés, perdent la traction et se coulent dans le liquide riche en enzymes digestifs au dessous (14). Les substances nutritives des cadavres sont absorbées par la plante en compensant pour la lacune d’azote dans le sol. Les couvercles des urnes expriment une couleur rouge ou verte. Notre étude examine les différences chimiques et …

Differences In Pah Tolerance Between Capitella Species: Underlying Biochemical Mechanisms, Lis Bach, Annemette Palmqvist, Lene Juel Rasmussen, Valery E. Forbes

Valery Forbes Publications

The polychaete Capitella capitata consists of a species complex within which differences in tolerance to tox­icants have been observed. For example, it has been shown that Capitella sp. S is more sensitive (e.g., in terms of survival, growth and reproduction) to PAH and other stressors than the more opportunistic Capi­tella sp. I, which is able to take up and biotransform the PAH fluoranthene (Flu). In the present study, an in­vestigation was performed to examine whether differences in tolerance between Capitella species sp. I and sp. S are due to differences in biotransformation, measured as the amount of Flu-metabolites produced by …

The Effects Of An Analgesic (Biofreeze®) On The Motoneuron Pool Excitability Of The Soleus Muscle, Desiree R. Barth

All-Inclusive List of Electronic Theses and Dissertations

Objective: The purpose of this study was to determine the effects on the Hoffman reflex and motor response with an analgesic (Biofreeze®). Design: The study was a 2X6 design. The independent variables in this study were treatment and time. The dependent variables were the H-reflex (volts) and the M response (volts). Treatment was randomly assigned to control for the effects of order. Setting: This study took place in the Exercise Physiology Laboratory located in the Health and Human Performance Building at Indiana State University. Subjects: 14 volunteers who were physically active and had no known neurological problems. Measurements: Skin and …

Risk And Consequence Analysis Focused On Biota Transfers Potentially Associated With Surface Water Diversions Between The Missouri River And Red River Basins, Greg Linder, Ed Little, Lynne Johnson, Chad Vishy, Bruce Peacock, Heather Goeddecke

United States Geological Survey: Publications

Section 1 provides a brief overview of the project, including a cursory summary of the history of the “Garrison Diversion” and how that history relates to this work focused on the analysis of risks and consequences potentially associated with interbasin biota transfers. The present study was initiated under the auspices of the Dakota Water Resources Act (DWRA) of 2000, which directed the Secretary of the Interior to conduct a comprehensive study of the water quality and quantity needs of the Red River Valley and the options for meeting those needs. As such, the Bureau of Reclamation (Reclamation) requested technical support …

Manganese Distribution Across The Blood-Brain Barrier. Iv. Evidence For Brain Influx Through Store-Operated Calcium Channels, Janelle S. Crossgrove, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

Manganese (Mn) is a required co-factor for many ubiquitous enzymes; however, chronic Mn overexposure can cause manganism, a parkinsonian-like syndrome. Previous studies showed Mn influx into brain is carrier-mediated, though the putative carrier(s) were not established. Studies conducted with cultured bovine brain microvascular endothelial cells (bBMECs), which comprise the blood–brain barrier, revealed ⁵⁴Mn (II) uptake positively correlated with pH, was temperature-dependent, and was sodium- and energy-independent. Brain ⁵⁴Mn uptake correlated inversely with calcium (Ca) concentration, but ⁴⁵Ca uptake was unaltered by high Mn concentration. Lanthanum (La), a non-selective inhibitor of several Ca channel types, as well as …

Prenatal Cocaine Exposure And Cardiac Programming, Soochan Bae

Loma Linda University Electronic Theses, Dissertations & Projects

Human epidemiological studies have shown a clear association of adverse intrauterine environment and an increased risk of ischemia heart disease in later adult life. Cocaine abuse during pregnancy has been shown to cause abnormities in the heart during fetal and postnatal development, but mechanisms underlying the detrimental effects of cocaine on the developing heart are not fully understood. The central hypothesis is that cocaine exposure during fetal development may cause in utero programming of apoptotic pathways, which may has lasting and prolong effect on heart development postnatally.

In a pregnant rat model, chronic hypoxia increased apoptosis in the fetal heart …

Environmental Factors In Autoimmunity: Assessment Of Immunotoxicity Of Phthalates In The Induction Of Lupus-Type Anti-Dna Antibodies, So-Yon Lim

All-Inclusive List of Electronic Theses and Dissertations

Objectives: Little is known of the role of specific environmental factors in promoting autoimmune disorders such as systemic lupus erythematosus (SLE). The broad objective of this study was to address how exposure to phthalates, common environmental factors m foods, and biomedical devices could affect the immune functions of resistant and autoimmune-prone mice. Methods: The environmental chemical model used in this study is phthalates, which are widely used in various forms and readily occur during day-to-day human contacts. Groups of nonautoimmune-prone (BALB/c, DBA/2 and C57BL/6) and susceptible (NZB, NZB/W Fl) mice were immunized with phthalates ( ortho, meta and para) conjugated …

Protective Effects Of Ginseng Components In A Rodent Model Of Neurodegeneration (Abstract), Xiao-Yuan Lian, Zhizhen Zhang, Janet L. Stringer

NCPC Publications and Patents

No abstract provided.

Harmful Garden Plants In Western Australia, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

Many garden plants can cause harm. Some are commercially available and very popular; others are no longer readily available but still exist in older gardens; and yet others are favourite indoor ornamentals, cut-flowers, weeds, or even fruit and vegetables that we consume frequently, often without realising that other parts of those same plants are harmful.

It is impractical and unnecessary to remove from our gardens every single plant that could conceivably be harmful. A more sensible approach is to be aware of the potential danger of a particular plant, and then assess how much or how little risk it poses …

In Vitro Toxicity Of Cadmium Oxide Particles In Brl 3a Rat Liver Cells, Janice M. Shelley

Theses and Dissertations

For the last decade or so, in vitro toxicology has been studied as a method for determining human toxicity, as well as hazard identification and characterization. One purpose of in vitro studies is to determine the possibility of developing systems in which the toxicity of chemicals can be quantified, without heavy reliance on animal experimentation. Specific data pertaining to cadmium toxicity in humans through use of in vitro methods and models is sparse, at best. There is a need to complete more studies and collect more data to study particular chemicals and their effects and to complete proper evaluation and …

Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts, Arati Limaye

Electronic Theses and Dissertations

The skyrocketing costs of prescription medicine in developed countries and their lack of availability in developing countries are the most challenging problems of human health. Primary reasons for such high cost are fermentation-based production, expensive purification methods, the need for low temperature storage and transportation and the delivery through sterile injections. Most of these expenses could be minimized or eliminated when therapeutic proteins are expressed and orally delivered via plant cells. Chloroplasts have the machinery to fold complex and biologically active eukaryotic proteins in the soluble chloroplast stromal compartment. Protein expression through chloroplast transformation system offers a number of advantages …

Discovery Shook Medical, Pharmaceutical Industries, Aldemaro Romero Jr., David Gilmore

Publications and Research

No abstract provided.

Takagi–Sugeno Fuzzy Modeling Of Skin Permeability, Deepak R. Keshwani, David D. Jones, Rhonda M. Brand

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The skin is a major exposure route for many potentially toxic chemicals. It is, therefore, important to be able to predict the permeability of compounds through skin under a variety of conditions. Available skin permeability databases are often limited in scope and not conducive to developing effective models. This sparseness and ambiguity of available data prompted the use of fuzzy set theory to model and predict skin permeability. Using a previously published database containing 140 compounds, a rule-based Takagi–Sugeno fuzzy model is shown to predict skin permeability of compounds using octanol-water partition coefficient, molecular weight, and temperature as inputs. Model …

Skin Permeation Enhancement Of Ketoprofen Supersaturated Solutions With Antinucleant Polymers(การเพิ่มการซึมผ่านผิวหนังของสารละลายอิ่มตัวยิ่งยวดของ..., Tanasait Ngawhirunpat, Praneet Opanasopit, Suwannee Panomsuk, Theerasak Rojanarata, Usanee Kumprakrob

The Thai Journal of Pharmaceutical Sciences

Skin permeation enhancement of ketoprofen (KP) from supersaturated systems and effects of three antinucleant polymers, polyvinylpyrrolidone (PVP), hydroxypropylmethylcellulose (HPMC) and sodium carboxymethylcellulose (SCMC), on stability and skin permeat...

Antimutagenicity Of 4-Hexylresorcinol Against Urethane In Somatic Mutation And Recombination Test(ผลของการต้านฤทธิ์ก่อกลายพันธุ์ของ 4-เฮ็กซิลรีซอร์ซินอล..., Kaew Kangsadalampai, Suphaphan Soiphet

The Thai Journal of Pharmaceutical Sciences

This study examined the effect of 50, 500 and 5000 ppm of 4-hexylresorcinol (4-HR) on the mutagenicity of 2500 ppm urethane (URE) through somatic mutation and recombination testing using a high bioactivation cross of Drosophila melanogaster as the indica...

Effects Of Monascus Colorants On The Mutagenicity Of Nitrite-Treated 1-Aminopyrene Using Ames Test(ผล ของส ีท ี่ได ้จ าก เชื้อ รา Monascus ต่อฤทธก,อ ก ล ายพ ัน ธ ุข อ ง..., Kalyarat Kruawan, Kaew Kangsadalampai, Busaba Yongsmith, Thayat Sriyapai

The Thai Journal of Pharmaceutical Sciences

We studied the antimutagenicity of natural colorants synthesized by Monascus purpureus (ATCC 16365, NRRL 2897) and unidentified Monascus sp. (KB13.1, KB10M16 and KB20M10.2) against the reaction product (nitrite-treated 1-aminopyrene) produced from the re...

Properties Testing Of The Retranslated Sf-36 (Thai Version)(การทดสอบคุณสมบัติของแบบสอบถาม Sf-36 ภาษาไทยฉบับแปลใหม่), Watcharee Leurmarnkul, Paranee Meetam

The Thai Journal of Pharmaceutical Sciences

Quality of life assessment is an outcome measurement that has been increasingly focused by clinicians. At present, SF-36 (Thai version) is a widely used instrument. We previously reported validation results of the SF-36 which was proved unsatisfactory on...

Evaluation Of Piroxicam Gel Formulations: Comparison Between In Vitro Permeation, In Vivo Pharmacological And Skin Stripping Techniques(การประเมินสูตรตำรับไพรอกซิแคมเจล : การเปร..., Parkpoom Tengamnuay, Witthaya Janthasoot, Maytinee Limsiriwong

The Thai Journal of Pharmaceutical Sciences

Purpose. To evaluate the topical bioavailability and efficacy of various piroxicam gel products using in vitro permeation, in vivo pharmacological and skin stripping techniques and to compare these methods for possible correlation. Methods. Four commerci...

Key Word Index, Author Index(ดรรชนีกุญแจคำ, ดรรชนีผู้แต่ง), N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Development And Testing Of Thai Smoking Cessation Pharmaceutical Therapy-Related Quality Of Life Assessment Instrument(การสร้างและทดสอบเครื่องมือประเมินคุณภาพชีวิตจ..., Supakit Wongwiwatthananukit, Rawadee Dhumma-Upakorn, Thitipom Naktuan

The Thai Journal of Pharmaceutical Sciences

The purpose of this methodological research was to develop a smoking cessation pharmaceutical therapy-related quality of life assessment instrument and examine the validity and reliability of this measure. This research involves 3 steps: (1) instrument d...