Pharmacology, Toxicology and Environmental Health Commons^™

Toxicological Effects And Treatment Of Accidental Ingestion Of Simparica Trio® In Felis Catus: An Investigative Review, Aussia Stander

Honors Theses

Simparica TRIO^®, a canine endectocide manufactured by Zoetis, contains sarolaner, moxidectin, and pyrantel pamoate salt in an orally administered chewable tablet. These compounds are all toxic to both cats and dogs in sufficient amounts. As Simparica TRIO^® rises in popularity among pet owners, Felis catus (felines, cats) in multi-pet households (households with both cats and dogs) are at increased risk of overdosage and toxicosis from accidental ingestion of Simparica TRIO^® due to their smaller weight.

Simparica TRIO^® is not for feline use and little literature exists on the symptoms of and treatment for accidental ingestion …

An Analysis Of Pollution In Liverpool, Uk, Luke W. Lawson

Honors Theses

Within this experiment the different levels of pollution in areas around Liverpool were tested. These areas were Stanley Park, Lunt Meadows, and Woolton Road. At Stanley Park one of two transects had a geochemical analysis as well as magnetic measurements run to investigate source attribution within the urban environment resulting in data showing high variability in soil properties among the transect. With data supporting evidence of diamagnetic, paramagnetic, and ferromagnetic properties in soil along the same transect. At Lunt Meadows, through the use of XRF for soil samples and nitrate and phosphate testing for water samples, it was found that …

Monitoring The Leaching Of Bisphenol-A From Feminine Hygiene Products Using Fluorescence Spectroscopy, Madison Easley

Honors Theses

Bisphenol-A (BPA) is a compound commonly used as a stabilizer in plastic products, including food storage containers and thermal paper receipts. Because BPA can bind to and activate estrogen receptors, it is linked to reduced fertility, altered development, and hormone-related cancers. A recent study at NYU Medical School confirmed the presence of BPA in pantyliners, pads, tampons, feminine washes and deodorants. This is concerning due to the high absorption capacity of the vulvar skin.

For the research performed in our lab this summer, the goal was to determine if fluorescence spectrophotometry could be used to determine the presence of BPA …

The Effect Of Cannabinoids And Terpenes On The Activation Of Hmc3 Microglial Cells, Mohamed Marzouk

Honors Theses

Life expectancies of people living with HIV have significantly lengthened due to the availability of antiretroviral therapies. Despite their ability to increase survival, these treatments do not “cure” HIV, nor do the stop the onset of neurological symptoms associated with infection, termed neuroHIV. NeuroHIV describes a myriad of neurological impairments including mood disorders (depression and anxiety), cognitive impairment, neuropathic pain, and motor disinhibition that reduce quality of life for people living with HIV. Mechanistically, the neurological impairments may involve actions of neurotoxic proteins directly produced by the virus. One of these proteins that has been well-characterized is the HIV trans …

Cats Of Unl: An Investigation Into The Human-Feral Cat Relationships On A University Campus In Nebraska, Jacey Skoda

Honors Theses

At the University of Nebraska-Lincoln (UNL), there exists a population of feral cats that is monitored and cared for by a group known as Husker Cats. As the cats wander the sidewalks and gardens of the university, they may appear non-threatening and pleasant to have around. However, a feral cat presence could have detrimental impacts on the surrounding ecosystem, property, and community. The first component of this project, a survey of individuals on UNL campus, ultimately seeks to understand what those in the UNL community know about the feral cats, how they feel about the feral cats, and how they …

Early Life Δ9-Tetrahydrocannabinol Exposure Of F0 Zebrafish Causes Hyperactivity In F1 Offspring, Jenna Cripe

Honors Theses

As cannabis increases in its accessibility, potency, and acceptance across the United States, investigation into the multigenerational effects of Δ9-tetrahydrocannabinol (THC) exposure during key stages of development is critical. The aim of this study was to evaluate whether there would be behavioral impacts in the F1 offspring following a dose-response of THC exposure (0.08, 0.4, or 1 µM) during development in the F0 generation. Zebrafish (Danio rerio) were utilized in this study. Behaviors (locomotive activity and anxiety-like behavior) in the F1 generation were evaluated at 120 hours post-fertilization (hpf) with the larval photometer response (LPR) assay, and at 3, 11, …

Cortisol Receptor Sensitivity As A Risk Factor For Depression, Michela Michielli

Honors Theses

In 2020, the World Health Organization reported over 264 million people across the world were suffering from depression. Studies have demonstrated that one source of depression is a hormonal imbalance involved in the stress response. Cortisol is a stress hormone regulated by the Hypothalamic-Anterior-Pituitary (HPA) Axis. Its effects on the stress response and other metabolic activities in the body are exerted through the glucocorticoid and mineralocorticoid receptors (GR and MR respectively).

Our research has examined mutations known as single-nucleotide-polymorphisms (SNPs) relating to cortisol-receptor sensitivity and the behavior of cortisol in the body to investigate the link between cortisol activity and …

The Synthesis And Characterization Of Ionic Liquids Using Nitrogen-Based Cations For Transdermal Delivery, Jo Galloway

Honors Theses

The purpose of this research was to explore the synthesis mechanisms of water-soluble ionic liquids with nitrogen-containing cationic bases for future use in transdermal drug delivery and forensic science applications. Ionic liquids are salts with an organic cation and either an organic or inorganic anion. They have asymmetric structures, which means that the molecules don’t pack together as neatly as other salts do, therefore, they don’t crystallize as easily, and their melting points are lower. Ionic liquids have melting points below 100°C, and many are liquid at room temperature. Mechanistic studies reveal that the potency of ILs in enhancing transdermal …

Finding The Optimal Ionic Liquid To Target White Blood Cell Subpopulations Using Nanoparticles, Meghan Gorniak

Honors Theses

Disorders and diseases of the immune system have become more prevalent in recent decades and can have life-threatening effects on those afflicted. Granulocyte disorders disproportionately affect infants and children, while chronic lymphocytic leukemia is the most common type of leukemia in adults. Unfortunately, current treatments have many drawbacks such as everyday injection, short-lived efficacy, and unknown levels of safety and effectiveness. It is imperative to find more effective treatment options that could allow for easier drug delivery to specific populations of white blood cells (WBCs). Nanoscale medicine shows promising application, but complications remain in reaching the desired target site and …

Fast Photochemical Oxidation And Footprinting Of Proteins Via Trifluoromethyl Radical Chemistry, Elaine Morrow

Honors Theses

Fast photochemical oxidation of proteins (FPOP) is a useful tool in proteomics because of the ability for modifications to occur on the scale of microseconds which reduces the modifications to tertiary and quaternary structure allowing for more accurate labeling of the protein. Labels for FPOP are generated from various radicals in our experiments which include hydroxyl radicals and trifluoromethyl radicals. Hydroxyl radicals are easily generated by using an excimer laser (KrF laser, 248 nm) or a UV flash lamp (as a part of the Fox™ System) by the photolysis of hydrogen peroxide. Trifluoromethyl radicals, however, need hydroxyl radicals to be …

Black Carbon And Meteorological Parameters At Two Locations In Northern Mississippi, Ashton Swader

Honors Theses

Black Carbon, which is a component of fine particulate matter, is a known pollutant that has been linked to the development of several diseases including cardiovascular disease. This pollutant can be measured by taking samples of air within a certain region and analyzing them using a transmissometer. It is known that black carbon concentrations tend to be higher in urban areas when compared to rural areas within a region. Black carbon concentrations can also vary depending on certain meteorological parameters such as temperature and humidity. There were no current studies that analyze the air quality in northern Mississippi due to …

Sex Differences In Lateral Hypothalamic Extracellular Glucose Concentrations During Cumulative Dosing Of Methamphetamine In Rats, Joshua A. Jolton

Honors Theses

The present study experimentally investigated sex differences in lateral hypothalamic glucose concentrations following cumulative dosing of methamphetamine. Male (n=17) and female (n=11) rats were surgically implanted with enzyme-based glucose biosensors in the lateral hypothalamus. Rats then received a saline injection followed by four methamphetamine doses (0.025 mg/kg, 0.05 mg/kg, 0.1 mg/kg, and 0.2 mg/kg) spaced 55 minutes apart. We found that while there was a general decrease in glucose baseline throughout the study for both sexes, the magnitude of the decrease was significantly greater in females compared to males. On a rapid timescale, fixed sex effects existed at the lower …

Attempted Synthesis & Antibacterial Properties Of Apt-6k Against Ndm-1 K. Pneumoniae, Alec Bofetiado

Honors Theses

NDM-1 K. pneumoniae is a highly resistant bacterial organism that is capable of causing debilitating nosocomial infections in immunocompromised patients. Only "last-resort" antibiotics--such as colistin--work against this organism. Therefore, new antibiotics are needed to help fight against these types of infections. APT-6K is a novel compound that was demonstrated to be effective against MRSA with nanomolar concentrations in a prior study. Novel methods of APT-6K synthesis and its testing for antibiotic effects against NDM-1 K. pneumoniae were attempted in this research. APT-6K synthesis was unsuccessful. Commercially-prepared APT-6K also did not demonstrate growth inhibition against NDM-1 K. pneumoniae nor against a …

Pfas Environmental Contamination In Central Maine, Megan H. Andersen

Honors Theses

PFAS (per- and polyfluoroalkyl substances) are toxic, persistent, and bioaccumulative chemicals that are used in a variety of consumer products and industrial applications. Paper mills across the state of Maine have used PFAS in their industrial paper making processes, and their wastes often flow for treatment into municipal sewage treatment facilities. PFAS contaminated municipal wastewater from the general use of PFAS consumer products are also processed by these plants. Liquid waste is condensed into solid waste septage and sludge, and in what was thought to be an environmentally friendly repurposing practice, municipal sludge and septage has been spread on plots …

Examining An Intersection Of Environmental Justice And Covid-19 Risk Assessment: A Review, Ashley Ellis

Honors Theses

This study views the risks associated with the novel coronavirus (COVID-19) as an environmental injustice issue due to the connection between existing environmental disparities and the disproportional negative impacts brought upon by the virus. The social and health determinants attributed to those environmental disparities have traditionally been evaluated as individual risk factors, an approach that fails to gauge the complexity of an environmental injustice issue. This study employs the emerging theory of intersectionality, a belief that phenomena cannot be linked to one principal cause but instead an interconnected web of influences, in order to synthesize the multitude of factors believed …

Environmental Justice Analysis Of Drinking Water Policies In Michigan Cities, Annika Paldan

Honors Theses

This thesis examined environmental justice aspects of drinking water policies in four cities across Michigan: Flint, Birmingham, St. Joseph, and Benton Harbor. An overview of the history of the environmental justice movement, environmental racism, and drinking water policies provided the basis for a four-part evaluative criterion to assess environmental justice components at the municipal level. These criteria include (1) housing tenure, (2) age, size, and service line composition of the infrastructure, (3) public participation in the policy process, and (4) emergency management of the city. Findings indicate that environmental justice has come a long way, with cities now incorporating components …

Ecoturismo Y Empoderamiento: La Ecoinnovación De Las Mujeres Indígenas En América Latina, Kathleen Sinatra

Honors Theses

This thesis focuses on the role of indigenous women in relation to ecotourism efforts and ecoinnovation. Through an analysis of three case studies, I discuss the need to account for both indigenous people and gender when engaging in ecotourism efforts. I first engage in a literature review that develops the complex relationship between the identity factors of indigeneity and gender. I then look at a case study of the hotel Taselotzin in Cuetzalan del Progreso, Mexico in which a cooperative group of indigenous women founded a hotel. I then turn to the Kichwa community in Ecuador and the organization Amukishmi …

Determination Of Metals And Microplastics In Sediment From Oyster Reefs In The Mississippi Sound, Shelby Goza

Honors Theses

This research centers on the determination of metals (Cd, Pb, V, Cr, Mn, Fe, Co, Ni, Cu) and microplastics (MPs) in marine sediment from oyster reefs in the Mississippi Sound Estuary. Oysters (Crassostrea virginica) are a vital part of the Gulf Coast economy, but their population has dramatically declined in recent years due to multiple stressors, including oil spills and fluctuations in salinity from flooding. Increasingly frequent flooding events also introduce high loads of MPs and sediments containing heavy metals from the Mississippi River. These pollutants can have deleterious effects on oyster biology; however, they have not been adequately …

Composition And Oxidative Potential Of Fine Particulate Matter From Pascagoula, Ms, Madison Smith

Honors Theses

Air pollution is one of the top-ranking risk factors for death and disability around the world, accounting for nearly 4.9 million deaths worldwide (State of Global Air, 2019). Specifically, fine particulate matter (PM_2.5) has been linked to many adverse effects on human health, yet there is very little known about what characteristics exactly cause these adverse health effects and how they cause them. Samples for this study were collected from Pascagoula, MS, a small town located on the Mississippi Gulf Coast that is home to many industrial yards, from September 2013 to December 2013. Black carbon (BC) analysis …

Responses To Fine Particulate Matter (Pm2.5) Exposures In Two Wild-Type Zebrafish Strains, Connor Necaise

Honors Theses

Atmospheric fine particulate matter (PM_2.5) exposure poses great health risks across the globe, causing both acute and chronic illnesses in humans. Therefore, a more complete understanding of the mechanisms in which PM_2.5 induces these adverse health effects is urgently needed. Oxidative stress due to PAHs and other common components of PM_2.5 is a proposed mechanism for its adverse health effects. However, little is known about the actual mechanisms of PM_2.5 damage in humans. This study aimed to distinguish behavioral differences in two lines of zebrafish (AB & 5D) as a result of developmental exposure to …

Lc-Ms/Ms Method Development And Analysis Of Tricyclic Antidepressants In Human Plasma, Shahbaz Gul

Honors Theses

Depression has become one of the most prominent problems in society, disrupting both personal and social lives. Tricyclic antidepressants (TCAs) were first developed in the 1950’s and became some of the leading anti-depressant medications on the market later that decade. Today, newer anti-depressants have risen to the forefront, being safer and having a lower side-effect probability. Nonetheless, TCAs continue to be prescribed for severe depression, especially in cases where the newer anti-depressants have failed. However, TCAs are still highly potent, the toxicity associated with these compounds cannot be ignored. They have considerable cardiovascular and neurological toxicity, and in the event …

Narrative Storytelling In Conjunction With Environmental Journalism, Abbey Edmonson

Honors Theses

This thesis explores the effectiveness of long-form narrative storytelling in relation to environmental journalism. It particularly focuses on effectively communicating the seriousness of climate change on a local level through narrative storytelling. The bulk of the information was gathered through multiple interviews with people experiencing the effects of climate change on the Mississippi Gulf Coast. By using interviews with local people interspersed with scientific data, I hope to be able to show the average reader that climate change is real and it can dangerously affect people on a local level, even in rural states like Mississippi.

Through my research, I …

Examining Public Water Systems Servicing Schools And Childcare Facilities To Inform Policy: A Study Of Lead Exposure In The Mississippi Delta, William Farmer

Honors Theses

Abstract

This study was initiated with the intent of identifying elementary schools and childcare facilities in the Mississippi Delta with children ages 6 and under that may have public water systems (PWSs) with lead exceedances of 5 ppb. We also aimed to identify the existence, and further the availability, of information regarding which public schools and childcare facilities were serviced by which public water systems. Based on the results of this study, considerations related to Mississippi’s ability to comply with the 2020 Revisions of the Lead and Copper Rule requiring testing in all public schools were determined. Seven principal counties …

Oxidative Potential And Composition Of Fine Particulate Matter At Two Locations In Mississippi, Victoria Claire Stevens

Honors Theses

Air pollution exposure has negative health effects on individuals across the globe. Negative impacts on human health have been documented in studies following both short-term and long-term exposure to air pollution, and these adverse effects have been seen even at the lowest levels of exposure. Fine particulate matter (PM_2.5) has specifically been linked to these negative health effects. These effects come from many sources, but one large contribution to the negative health effects comes from oxidative stress. In this study, samples were collected from Hernando, MS and Gulfport, MS in September 2013 and November 2013. Black carbon (BC) …

Characterizing Pm2.5 Air Samples Of Northeast Mississippi, Harley Nabors

Honors Theses

Ambient air pollution has been associated with adverse health effects throughout the entire world. Although epidemiologists study air pollution extensively, rural areas are often the least understood. In 2020, the Mississippi Department of Environmental Quality used less than 10 air sampling locations across the state leaving a large gap in air quality knowledge. In this study, fine particulate matter (PM2.5) air samples were collected from three different rural Mississippi cities–Ackerman, Eupora, and Houston, in the summer of 2020. Following collection onto filters, elemental black carbon analysis and an oxidative potential assay were both used to characterize the samples of interest. …

Surface-Eroding Drug Delivery Films For Sequential And/Or Intermittent Release Of Psychoactive Drugs, Jason Price

Honors Theses

ABSTRACT JASON BLAKE PRICE: Surface-Eroding Drug Delivery Films for Sequential and/or

Intermittent Release of Psychoactive Drugs For patients with psychiatric diseases, adherence to medication schedules, medication

errors, and abuse are common issues. Promising new forms of therapy for these patients, such as micro-dosed lysergic acid diethylamide (LSD), where patients receive 10-20% of a full dose every third day, present further drug delivery challenges. Sequential or intermittent release of drugs from an implanted device could ensure long-term drug compliance, automate drug dosing during the life of the implant, and eliminate potential for abuse and medication errors. To this end, we generated …

Use Of Small Molecule Fanconi Anemia Pathway Inhibitors As Sensitizing Agents To Laromustine., Sam W. Marchant

Honors Theses

Laromustine is an experimental chemotherapeutic sulfonyl hydrazine prodrug shown in clinical trials to be effective against acute myeloid leukemia. The mechanism of action of laromustine involves interstrand crosslinking, via chloroethylation, and enzyme inhibition, caused by carbamoylation. The work described herein aims to investigate whether inhibition of the replication-dependent interstrand crosslink repair Fanconi Anemia pathway further sensitizes cells to laromustine. By measuring metabolic activity immediately after drug exposure, we find laromustine to be equally as cytotoxic towards Fanconi Anemia deficient and wild type cells. However, through clonogenic assays we show Fanconi Anemia mutations sensitize cells to laromustine’s anti-proliferative effect. Furthermore, we …

Pharmacy: The Backbone Of Healthcare, Jordan Raye Myers

Honors Theses

The field of pharmaceuticals is growing and changing everyday. Being a part of the creation of new medicines and new treatments is a dream. Throughout my life my favorite subject has always been science, specifically where the sciences of biology and chemistry intersect. Biology and chemistry have significant overlap in the field of pharmacy. I believe that the passion and love I have for combining these two sciences will reflect in my career as a pharmacist and open doors for me to positively impact the lives of patients and the pharmaceutical field. I chose to pursue a career in pharmacy …

Hiv-1 Tat Interactions With Opioids Are Modulated By Progesterone And Estradiol, Dejun Jackson

Honors Theses

HIV infection and combined substance abuse are comorbid epidemics. Previous studies show that concurrent opioid drug use may potentiate HIV-1-mediated neurotoxicity partly via interactions with opioids. Preclinical studies suggest that the HIV-1 trans-activator of transcription (Tat), an HIV regulatory protein, can synergize with opioids to exacerbate its already neurotoxic effects. However, its interactions with clinical opioids, such as oxycodone, have yet to be elucidated. Additionally, Tat disrupts a number of systems including the dopaminergic system, which contribute to its capacity to potentiate the rewarding effects of abused drugs. Although the neurotoxic effects of Tat may be inhibited by gonadal steroids …

The Impact Of Age/Rage Signaling On Oxidative Stress Under Diabetic Conditions In Cardiac Fibroblasts, Christopher Dorroh

Honors Theses

Diabetes is a major health concern in the United States, with 1.5 million new cases diagnosed each year. Patients who suffer from diabetes have an increased risk of developing heart failure, a form of cardiovascular disease. Heart failure has been shown to result from increased left ventricular stiffness, which in turn is caused by increased remodeling of the extracellular matrix (ECM). This increase in ECM remodeling is a result of AGE/RAGE signaling, which occurs at a heightened level in the cardiac fibroblast cells of diabetics. Studies have shown that diabetics have elevated levels of AGEs (Advanced Glycation End-Products), which bind …