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Articles 1 - 30 of 75
Full-Text Articles in Pharmacology, Toxicology and Environmental Health
Development Of The Navigation Guide Evidence-To-Decision Framework For Environmental Health: Version 1.0, Nicholas Chartres, Max T. Aung, Sustan L. Norris, Courtney Cooper, Lia A. Bero, Roger Chou, Devon C. Payne-Sturges, Wendy E. Wagner, Jessica W. Reyes, Lisa M. Askie, Daniel A. Axelrad, Deysi Flores Vigo, Jill E. Johnston, Juleen Lam, Keeve E. Nachman, Eva Rehfuess, Rachel Rothschild, Patrice Sutton, Lauren Zeise, Tracey J. Woodruff
Development Of The Navigation Guide Evidence-To-Decision Framework For Environmental Health: Version 1.0, Nicholas Chartres, Max T. Aung, Sustan L. Norris, Courtney Cooper, Lia A. Bero, Roger Chou, Devon C. Payne-Sturges, Wendy E. Wagner, Jessica W. Reyes, Lisa M. Askie, Daniel A. Axelrad, Deysi Flores Vigo, Jill E. Johnston, Juleen Lam, Keeve E. Nachman, Eva Rehfuess, Rachel Rothschild, Patrice Sutton, Lauren Zeise, Tracey J. Woodruff
Articles
Environmental exposures, including widespread industrial pollution, impact human health and are amplified in more highly exposed communities. Policy and regulatory frameworks for making decisions and recommendations on interventions to mitigate or prevent exposures tend to narrowly focus on exposure and some health-related data related to risks. Typically, such frameworks do not consider other factors, including essentiality, health equity, and distribution of benefits and costs. Further, decisions and recommendations lack transparency regarding how they were developed. We developed the Navigation Guide Evidence-to-Decision Framework for Environmental Health (E2DFEH) to provide a structured and transparent framework incorporating a range of scientific information and …
Confocal Raman Spectroscopy Coupled With In Vitro Permeation Testing To Study The Effects Of Formalin Fixation On The Skin Barrier Function Of Reconstructed Human Epidermis, Hichem Kichou, Franck Bonnier, Amanda C. Caritá, Hugh Byrne, Igor Choupra, Emilie Munnier
Confocal Raman Spectroscopy Coupled With In Vitro Permeation Testing To Study The Effects Of Formalin Fixation On The Skin Barrier Function Of Reconstructed Human Epidermis, Hichem Kichou, Franck Bonnier, Amanda C. Caritá, Hugh Byrne, Igor Choupra, Emilie Munnier
Articles
Confocal Raman Spectroscopy is recognised as a potent tool for molecular characterisation of biological specimens. There is a growing demand for In Vitro Permeation Tests (IVPT) in the pharmaceutical and cosmetic areas, increasingly conducted using Reconstructed Human Epidermis (RHE) skin models. In this study, chemical fixation of RHE in 10% Neutral Buffered Formalin for 24 hours has been examined for storing RHE samples at 4°C for up to 21 days. Confocal Raman Spectroscopy, combined with Principal Components Analysis, revealed the molecular-level effects of fixation, notably in protein and lipid conformation within the stratum corneum and viable epidermis. IVPT by means …
Photopolymer Material Durability And Safety In Holographic Diffusers For Visual Applications, Matthew Hellis, Alan Casey, Edoardo Splendi, Suzanne Martin, Matthew Sheehan, Kevin Murphy
Photopolymer Material Durability And Safety In Holographic Diffusers For Visual Applications, Matthew Hellis, Alan Casey, Edoardo Splendi, Suzanne Martin, Matthew Sheehan, Kevin Murphy
Articles
This study introduces novel holographic diffuser applications employing acrylamide- or diacetone acrylamide-based photopolymers, patterned within the volume on a micron scale by a single-beam holographic recording process. These diffusers have previously been presented as potential treatments for amblyopia and diplopia. This work has now been extended to spectrometric analysis to determine their properties under broadband light. Diffusive elements with higher diffusion efficiencies exhibited a marginal reduction (< 5%) in diffusion efficiency across most of the visible spectrum. Given the intended application of these holographic diffusers, cytotoxicity assessments were also performed. This is significant as there is a difference in toxicity between the crystalline acrylamide (classified as a category 3 material) and diacetone acrylamide (classified as a category 4 material). The findings indicated substantially lower toxicity in holograms produced with diacetone acrylamide-based photopolymer. The accelerated ageing of both formulations of holographic diffusers indicated that the acrylamide-based holographic diffusers did not reduce efficacy in the 292 days of ageing. The diacetone acrylamide-based holographic diffusers exhibited reduced efficacy by day 182. Despite this, both formulations have been shown to perform for prolonged periods as the treatment modality would require. These results emphasise that holographic diffusers exhibit minimal spectral impact, and longevity on the scale of treatment regimes which are crucial considerations for their prospective use case as treatments for amblyopia and diplopia.
Bioaccumulation Of As, Cd, Cr, Cu, Pb, Zn In Ambrosia Artemisiifolia L. In The Polluted Area By Enterprise For The Production And Processing Of Batteries, Ryzhenko Nataliia, El Amrani Abdelhak, Michelle Giltrap, Furong Tian, Volodymyr Laptev
Bioaccumulation Of As, Cd, Cr, Cu, Pb, Zn In Ambrosia Artemisiifolia L. In The Polluted Area By Enterprise For The Production And Processing Of Batteries, Ryzhenko Nataliia, El Amrani Abdelhak, Michelle Giltrap, Furong Tian, Volodymyr Laptev
Articles
In this paper, the concentration of As, Cd, Cr, Cu, Pb, Zn was investigated in soil and Ambrosia artemisiifolia L. sampling from polluted cite near the enterprises for the production and processing of batteries in the city of Dnipro in Ukraine. The obtained results of the study provided to assess plants regarding bio-monitoring and phytoremediation. Although Ambrosia artemisiifolia L. is a weed that causes serious allergic reactions in humans, this species can also have a high bioaccumulative capacity regarding metals. The metals’ concentration in roots was scientifically higher than in inflorescence part. Zn and Cu had the highest …
In Vitro Toxicological Evaluation Of Mesoporous Silica Microparticles Functionalised 1 With Carvacrol And Thymol, Cristina Fuentes, Ana Fuentes, Hugh Byrne, José Barat, María José Ruiz
In Vitro Toxicological Evaluation Of Mesoporous Silica Microparticles Functionalised 1 With Carvacrol And Thymol, Cristina Fuentes, Ana Fuentes, Hugh Byrne, José Barat, María José Ruiz
Articles
The cytotoxicity of carvacrol- and thymol- functionalised mesoporous silica microparticles (MCM-41) was assessed in the human hepatocarcinoma cell line (HepG2). Cell viability, lactate dehydrogenase (LDH) activity, reactive oxygen species (ROS) production, mitochondrial membrane potential (ΔΨm), lipid peroxidation (LPO) and apoptosis/necrosis analysis were used as endpoints. Results showed that both materials induced cytotoxicity in a time and concentration-dependent manner, being more cytotoxic than free essential oil components and bare MCM-41. This effect was caused by the cell-particle interactions and not from degradation products released to the culture media, as demonstrated in the extract dilution assays. LDH release was seen to be …
Biofabrication Of Magnetic Nanoparticles And Their Use As Carriers For Pectinase And Xylanase, Shady S. Hassan, Brendan Duffy, Gwilym A. Williams, Amit Jaiswal
Biofabrication Of Magnetic Nanoparticles And Their Use As Carriers For Pectinase And Xylanase, Shady S. Hassan, Brendan Duffy, Gwilym A. Williams, Amit Jaiswal
Articles
In this study, superparamagnetic iron oxide nanoparticles (MNPs) were synthesized via exposure of fungal cell filtrate from Aspergillus flavus to aqueous iron ions. The extracellular synthesis of MNPs was monitored by UV–Vis spectrophotometry and showed an absorption peak at 310 nm. The morphology of MNPs was found to be flake-like, as confirmed by Field Emission Scanning Electron Microscopy (FESEM), while the average crystallite size was ∼16 nm, as determined by X-ray diffraction (XRD). Energy dispersive X-ray (EDX) analysis was performed to confirm the presence of elemental Fe in the sample. Pectinase and xylanase were covalently immobilized on MNPs with efficiencies …
Application Of High-Intensity Ultrasound To Improve Food Processing Efficiency: A Review, Prasad Chavan, Pallavi Sharma, Sajeev Rattan Sharma, Tarsem Chand Mittal, Amit Jaiswal
Application Of High-Intensity Ultrasound To Improve Food Processing Efficiency: A Review, Prasad Chavan, Pallavi Sharma, Sajeev Rattan Sharma, Tarsem Chand Mittal, Amit Jaiswal
Articles
The use of non-thermal processing technologies has grown in response to an ever-increasing demand for high-quality, convenient meals with natural taste and flavour that are free of chemical additions and preservatives. Food processing plays a crucial role in addressing food security issues by reducing loss and controlling spoilage. Among the several non-thermal processing methods, ultrasound technology has shown to be very beneficial. Ultrasound processing, whether used alone or in combination with other methods, improves food quality significantly and is thus considered beneficial. Cutting, freezing, drying, homogenization, foaming and defoaming, filtration, emulsification, and extraction are just a few of the applications …
Advances In Emerging Technologies For The Decontamination Of The Food Contact Surfaces, Shubham Sharma, Swarna Jaiswal, Brendan Duffy, Amit Jaiswal
Advances In Emerging Technologies For The Decontamination Of The Food Contact Surfaces, Shubham Sharma, Swarna Jaiswal, Brendan Duffy, Amit Jaiswal
Articles
Foodborne pathogens could be transferred to food from food contact surfaces contaminated by poor hygiene or biofilm formation. The food processing industry has various conditions favouring microbes' adherence, such as moisture, nutrients, and the microbial inoculums obtained from the raw material. The function of the ideal antimicrobial surface is preventing initial attachment of the microbes, killing the microbes or/and removing the dead bacteria. This review article provides detail about the challenges food industries are facing with respect to food contact materials. It also summarises the merits and demerits of several sanitizing methods developed for industrial use. Furthermore, it reviews the …
Emerging Technologies For The Production Of Nanocellulose From Lignocellulosic Biomass, Dileswar Pradhan, Amit Jaiswal, Swarna Jaiswal
Emerging Technologies For The Production Of Nanocellulose From Lignocellulosic Biomass, Dileswar Pradhan, Amit Jaiswal, Swarna Jaiswal
Articles
Nanocellulose is a unique and promising natural nanomaterial and has gained significant attention due to its applications in several important areas. Thus, researchers are continuously looking for the most efficient, sustainable, economically viable, and environmentally friendly production technologies to fulfil its growing demand. Conventional production technologies, which include various physical, chemical, and physicochemical methods, are currently inadequate for this purpose and have several limitations such as long processing time, high energy consumption, low recovery of nanocellulose, and many others. To overcome these shortcomings, scientists have investigated the prospect of utilizing emerging processing technologies such as microwave irradiation, deep eutectic solvent, …
Seaweed Polysaccharide In Food Contact Materials (Active Packaging, Intelligent Packaging, Edible Films, And Coatings), Kalpani Perera, Shubham Sharma, Dileswar Pradhan, Amit Jaiswal, Swarna Jaiswal
Seaweed Polysaccharide In Food Contact Materials (Active Packaging, Intelligent Packaging, Edible Films, And Coatings), Kalpani Perera, Shubham Sharma, Dileswar Pradhan, Amit Jaiswal, Swarna Jaiswal
Articles
Food contact materials (FCMs) are materials that come in contact with food products such as food packaging which play a significant role in food quality and safety. Plastic, which is a major food packaging material, harms the ecosystem, wildlife, and the environment. As a result, numerous researches have been in progress on alternative polymers, which have similar properties as plastic but are also environmentally friendly (biodegradable). In recent years, the utilization of seaweed polysaccharides has piqued interest due to their biodegradability, non-toxicity, antioxidant capabilities, and excellent film formation ability. However, it has a number of drawbacks such as low tensile …
Structure Based Prediction Of A Novel Gpr120 Antagonist Based On Pharmacophore Screening And Molecular Dynamics Simulations, Ajay Pal Mr, Gemma K. Kinsella, James Curtin
Structure Based Prediction Of A Novel Gpr120 Antagonist Based On Pharmacophore Screening And Molecular Dynamics Simulations, Ajay Pal Mr, Gemma K. Kinsella, James Curtin
Articles
The G-protein coupled receptor, GPR120, has ubiquitous expression and multifaceted roles in modulating metabolic and anti-inflammatory processes. Recent implications of its role in cancer progression have presented GPR120 as an attractive oncogenic drug target. GPR120 gene knockdown in breast cancer studies revealed a role of GPR120-induced chemoresistance in epirubicin and cisplatin-induced DNA damage in tumour cells. Higher expression and activation levels of GPR120 is also reported to promote tumour angiogenesis and cell migration in colorectal cancer. Some agonists targeting GPR120 have been reported, such as TUG891 and Compound39, but to date development of small-molecule inhibitors of GPR120 is limited. …
Knowledge, Attitude And Practices (Kap) Towards Diet And Health Among International Students In Dublin: A Cross-Sectional Study, Xiyao Liu, Haoyue Chen, Qianling Zhou, Huifeng Zhang, Phensiri Asawasirisap, John Kearney
Knowledge, Attitude And Practices (Kap) Towards Diet And Health Among International Students In Dublin: A Cross-Sectional Study, Xiyao Liu, Haoyue Chen, Qianling Zhou, Huifeng Zhang, Phensiri Asawasirisap, John Kearney
Articles
International students may have difficulties in dietary acculturation. This study aimed to evaluate the knowledge, attitude and practices (KAP) of diet and health during the acculturation of international students. A cross-sectional survey was conducted among a convenience sample of 473 international students in Dublin. Knowledge, attitude and practices towards diet and health were evaluated by a questionnaire with open- and closed-ended questions. It was found that 45.3% of participants had a broad concept of a healthy diet, while few knew its specific contents. Furthermore, 75.3% of participants could explain the term functional food, and among them, 62.1% knew the appropriate …
In-Vitro Localisation And Degradation Of Few-Layer Mos2 Submicrometric Plates In Human Macrophage-Like Cells: A Label Free Raman Micro-Spectroscopic Study, Caroline Moore, Andrew Hrvey, Jonathan N. Coleman, Hugh Byrne, Jennifer Mcintyre
In-Vitro Localisation And Degradation Of Few-Layer Mos2 Submicrometric Plates In Human Macrophage-Like Cells: A Label Free Raman Micro-Spectroscopic Study, Caroline Moore, Andrew Hrvey, Jonathan N. Coleman, Hugh Byrne, Jennifer Mcintyre
Articles
Monitoring the uptake, micro-environment and fate of micro or nano scaled particulate materials in cells is of paramount importance for the emerging fields of toxicology and medicine. Such particulate materials are known to interfere with colorimetric assays and many such assays record only a single end-point. Therefore, there is a need for a label-free, cost effective technique with little or no inference from the particulate materials. Raman micro-spectroscopy was used to simultaneously interrogate the integrity of few-layer MoS2 submicrometric plates in human macrophage-like cells, in-vitro, as well as the biochemical characteristics of the local micro-environment in which they are encompassed. …
Data Mining Raman Microspectroscopic Responses Of Cells To Drugs In Vitro Using Multivariate Curve Resolution-Alternating Least Squares, David Perezguaita, Guillermo Quintas, Zeineb Farhane, Roma Tauler, Hugh Byrne
Data Mining Raman Microspectroscopic Responses Of Cells To Drugs In Vitro Using Multivariate Curve Resolution-Alternating Least Squares, David Perezguaita, Guillermo Quintas, Zeineb Farhane, Roma Tauler, Hugh Byrne
Articles
Raman microspectroscopy is gaining popularity for the analysis of time-dependent biological processes such as drug uptake and cellular response. It is a label-free technique which acquires signals from a large variety of components, including cell biomolecules and exogenous compounds such as drugs and nanoparticles, and is commonly employed for in vitro analysis of cells and cell populations with no labelling or staining required. By monitoring the changes to the Raman spectra of the cell as a result of a perturbing agent (e.g. exposure to a drug or toxic agent), one can study the associated changes in cell biochemistry involved in …
Potential Of Raman Spectroscopy For The Analysis Of Plasma/Serum In The Liquid State: Recent Advances, Drishya Rajan Parachalil, Jennifer Mcintyre, Hugh Byrne
Potential Of Raman Spectroscopy For The Analysis Of Plasma/Serum In The Liquid State: Recent Advances, Drishya Rajan Parachalil, Jennifer Mcintyre, Hugh Byrne
Articles
There is compelling evidence in the literature to support the application of Raman spectroscopy for analysis of bodily fluids in their native liquid state. Naturally, the strategies described in the literature for Raman spectroscopic analysis of liquid samples have advantages and disadvantages. Herein, recent advances in the analysis of plasma/serum in the liquid state are reviewed. The potential advantages of Raman analysis in the liquid form over the commonly employed infrared absorption analysis in the dried droplet form are initially highlighted. Improvements in measurement protocols based on inverted microscopic geometries, clinically adaptable substrates, data preprocessing and analysis, and applications for …
Atr-Ir Spectroscopy For Rapid Quantification Of Water Content In Deep Eutectic Solvents, Suha Elderderi, Charlotte Leman-Loubiére, Laura Wills, Sandra Henry, Dominique Bertrand, Hugh Byrne, Igor Chourpa, Cécile Enguehard-Gueiffier, Emilie Munnier, Abdalla A. Elbashir, Leslie Boudesocque-Delaye, Franck Bonnier
Atr-Ir Spectroscopy For Rapid Quantification Of Water Content In Deep Eutectic Solvents, Suha Elderderi, Charlotte Leman-Loubiére, Laura Wills, Sandra Henry, Dominique Bertrand, Hugh Byrne, Igor Chourpa, Cécile Enguehard-Gueiffier, Emilie Munnier, Abdalla A. Elbashir, Leslie Boudesocque-Delaye, Franck Bonnier
Articles
Natural Deep Eutectic solvents (NADES) are green, ionic solutions prepared by mixing vegetal cellular constituents. They are renewable and biodegradable solvents composed of hydrogen bonded donor and acceptor compounds. NADES have a wide range of applications, mainly as solvents for extraction of plants, biocatalysis, and nanoparticle synthesis. Water content is very critical, impacting significantly on NADES properties. Therefore, controlling H2O concentration appears essential for optimal use of NADES. In the present study, Attenuated Total Reflectance – infrared spectroscopy (ATR-IR) coupled to multivariate analysis, namely Partial Least Square s regression (PLSR), was investigated as a rapid, label free and cost-effective tool …
Structural Features Of 1,3,4-Thiadiazole-Derived Ligands And Their Zn(Ii) And Cu(Ii) Complexes Which Demonstrate Synergistic Antibacterial Effects With Kanamycin, Dariusz Karcz, Arkadiusz Matwijczuk, Daniel Kaminski, Bernadette Creaven, Katarzyna Lecka-Szlachta, Karolina Starzak
Structural Features Of 1,3,4-Thiadiazole-Derived Ligands And Their Zn(Ii) And Cu(Ii) Complexes Which Demonstrate Synergistic Antibacterial Effects With Kanamycin, Dariusz Karcz, Arkadiusz Matwijczuk, Daniel Kaminski, Bernadette Creaven, Katarzyna Lecka-Szlachta, Karolina Starzak
Articles
Classical synthetic protocols were applied for the isolation of three novel 1,3,4-thiadiazole derivatives which were then complexed with the biologically important Cu(II) and Zn(II) ions. All free ligands and their corresponding complexes were characterized using a number of spectroscopic techniques including Ultraviolet-visible (UV–vis), Fluorescence, Infrared (FT-IR), tandem liquid chromatography-mass (LC-MS), X-ray diffraction (XRD), and Nuclear Magnetic Resonance (NMR) spectroscopy (1H, 13C, HSQC, HMBC). The results obtained are consistent with the formation of dihydrate complexes, in which the chelation of the metal ion occurs via one of the thiadiazole nitrogen atoms and the deprotonated hydroxyl group of the …
Cold Atmospheric Plasma Induces Accumulation Of Lysosomes And Caspase-Independent Cell Death In U373mg Glioblastoma Multiforme Cells, Gillian Conway, Zhonglei He, Ana L. Hutanu, George P. Cribaro, Eline Manaloto, Alan Casey, Damien Traynor, Vladimir Milosavljevic, Orla Howe, Carlos Barcia, James T. Murray, Patrick J. Cullen, James Curtin
Cold Atmospheric Plasma Induces Accumulation Of Lysosomes And Caspase-Independent Cell Death In U373mg Glioblastoma Multiforme Cells, Gillian Conway, Zhonglei He, Ana L. Hutanu, George P. Cribaro, Eline Manaloto, Alan Casey, Damien Traynor, Vladimir Milosavljevic, Orla Howe, Carlos Barcia, James T. Murray, Patrick J. Cullen, James Curtin
Articles
Room temperature Cold Atmospheric Plasma (CAP) has shown promising efficacy for the treatment of cancer but the exact mechanisms of action remain unclear. Both apoptosis and necrosis have been implicated as the mode of cell death in various cancer cells. We have previously demonstrated a caspase-independent mechanism of cell death in p53-mutated glioblastoma multiforme (GBM) cells exposed to plasma. The purpose of this study was to elucidate the molecular mechanisms involved in caspase-independent cell death induced by plasma treatment. We demonstrate that plasma induces rapid cell death in GBM cells, independent of caspases. Accumulation of vesicles was observed in plasma …
Dough Properties And Baking Characteristics Of White Bread, As Affected By Addition Of Raw, Germinated And Toasted Pea Flour, Kim A. Millar, Catherine Barry-Ryan, Roisin Burke, Sinead Mccarthy, Eimear Gallagher
Dough Properties And Baking Characteristics Of White Bread, As Affected By Addition Of Raw, Germinated And Toasted Pea Flour, Kim A. Millar, Catherine Barry-Ryan, Roisin Burke, Sinead Mccarthy, Eimear Gallagher
Articles
Thermal and non-thermal processing may alter the structure and improve the techno-functional properties of pulses and pulse flours, increasing their range of applications in protein-enhanced foods. The effects of germination and toasting of yellow peas (Pisum sativum) on flour and dough characteristics were investigated. Wheat flour was substituted with raw, germinated and toasted pea flour (30%). The resulting bread-baking properties were assessed. Toasting increased dough water absorption and improved dough stability compared with germinated and raw pea flour (p < 0.05). This resulted in bread loaves with comparable specific volume and loaf density to that of a wheat flour control. Significant correlations between dough rheological properties and loaf characteristics were observed. Addition of pea flours increased the protein content of the breads from 8.4% in the control white bread, to 10.1–10.8% (p < 0.001). Toasting demonstrated the potential to improve the techno-functional properties of pea flour. Results highlight the potential application of pea flour in bread-making to increase the protein content.
Qualitative And Quantitative Analysis Of Therapeutic Solutions Using Raman And Infrared Spectroscopy, Alaa A. Makki, Franck Bonnier, Renaud Respaud, Fatma Chtara, Ali Tfayli, Clovis Tauber, Dominique Bertrand, Hugh Byrne, Elhadi Mohammed, Igor Chourpa
Qualitative And Quantitative Analysis Of Therapeutic Solutions Using Raman And Infrared Spectroscopy, Alaa A. Makki, Franck Bonnier, Renaud Respaud, Fatma Chtara, Ali Tfayli, Clovis Tauber, Dominique Bertrand, Hugh Byrne, Elhadi Mohammed, Igor Chourpa
Articles
Anticancer drugs are prescribed and administrated to an increasing number of patients on a daily basis. As a consequence, a number of concerns have been raised about the patient health and safety in the case that the drugs administered are not at the required concentration or even worse not the correct ones. Quality control of therapeutic solutions has therefore been extensively implemented in hospital environments, in order to avoid any failure in the intense workflow faced by administering pharmacists. In the present study, infrared (IR) and Raman spectroscopy have been employed for the analysis of 3 commercially available therapeutic solutions …
Two-Dimensional Correlation Analysis Of Raman Microspectroscopy Of Subcellular Interactions Of Drugs In Vitro, Hugh Byrne, Franck Bonnier, Zeineb Farhane
Two-Dimensional Correlation Analysis Of Raman Microspectroscopy Of Subcellular Interactions Of Drugs In Vitro, Hugh Byrne, Franck Bonnier, Zeineb Farhane
Articles
Two-dimensional (2D) correlation analysis is explored to data mine the time evolution of the characteristic Raman microspectroscopic signatures of the subcellular responses of the nucleoli of human lung cancer cells to the uptake of doxorubicin. A simulated dataset of experimental control spectra, perturbed with systematically time-dependent spectral changes, constituted by a short-term response which represents the initial binding of the drug in the nucleolus, followed by a longer term response of the organelle metabolism, is used to validate the analysis protocol. Applying 2D correlation analysis, the in phase, synchronous correlation coefficients are seen to contain contributions of both response profiles, …
Raman Spectroscopy Of Blood Plasma Samples From Breast Cancer Patients At Different Stages., H. F. Nargis, H. Nawaz, A. Ditta, T. Mahmood, N. Rashid, M. Muddassar, M. I. Majeed, H. N. Bhatti, M. Saleem, K. Jilani, Franck Bonnier, Hugh Byrne
Raman Spectroscopy Of Blood Plasma Samples From Breast Cancer Patients At Different Stages., H. F. Nargis, H. Nawaz, A. Ditta, T. Mahmood, N. Rashid, M. Muddassar, M. I. Majeed, H. N. Bhatti, M. Saleem, K. Jilani, Franck Bonnier, Hugh Byrne
Articles
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.
Numerically Modelling Time And Dose Dependent Cytotoxicity, Hugh Byrne, Marcus A. Maher
Numerically Modelling Time And Dose Dependent Cytotoxicity, Hugh Byrne, Marcus A. Maher
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Dose-response curves are fundamental tools of in vitro toxicology, extensively employed in toxicant or drug screening. They are expressed by a variety of end-points and assays, measured at different time-points in a choice of cell-lines, but are typically quantified only using the mean concentration for 50% response (e.g. drug efficacy or pathway inhibition) as an indicator of overall effect. However, the response is the result of a complex and dynamic cascade of events which occur between the initial exposure and the measured end-point, and the characteristic rates of the contributing stages govern the dose response and ultimately the measured characteristic …
Raman Spectroscopic Analysis Of Saliva For The Diagnosis Of Oral Cancer: A Systematic Review, Genecy Calado, Isha Behl, Amuthachelvi Daniel, Hugh Byrne, Fiona Lyng
Raman Spectroscopic Analysis Of Saliva For The Diagnosis Of Oral Cancer: A Systematic Review, Genecy Calado, Isha Behl, Amuthachelvi Daniel, Hugh Byrne, Fiona Lyng
Articles
Abstract Oral squamous cell carcinoma (OSCC) is one of the most common malignancies worldwide, and new protocols for routine and early detection are required. Raman spectroscopy is an optical based method that can provide sensitive and non-invasive real time detailed information on the biochemical content of a sample like saliva, through the unique vibrations of its constituent molecules and this is sensitive to changes associated with disease. A comprehensive systematic review of the available scientific literature related to Raman spectroscopy of human saliva for diagnosis of OSCC was performed. The 785 nm laser line was most applied wavelength along with …
Principal Components Analysis Of Raman Spectral Data For Screening Of Hepatitis C Infection, A. Dita, H. Nawaz, T. Mahmood, M. Tahir, N. Rashid, Muhammad Mudassar, A.A.H. Alsaadi, Hugh Byrne
Principal Components Analysis Of Raman Spectral Data For Screening Of Hepatitis C Infection, A. Dita, H. Nawaz, T. Mahmood, M. Tahir, N. Rashid, Muhammad Mudassar, A.A.H. Alsaadi, Hugh Byrne
Articles
In the current study, Raman spectroscopy is employed for the identification of the biochemical changes taking place during the development of Hepatitis C. The Raman spectral data acquired from the human blood plasma samples of infected and healthy individuals is analysed by Principal Components Analysis and the Raman spectral markers of the Hepatitis C Virus (HCV) infection are identified. Spectral changes include those associated with nucleic acidsat720 cm−1, 1077 cm−1 1678 (CO stretching mode of dGTP of RNA), 1778 cm−1 (RNA), with proteins at 1641 cm−1(amide-I), 1721 cm−1(CC stretching of proteins) and lipids at 1738 cm−1(CO of ester group in …
A Review Of Human Factors And Food Safety In Ireland, Ciara Walsh, Maria Chiara Leva
A Review Of Human Factors And Food Safety In Ireland, Ciara Walsh, Maria Chiara Leva
Articles
Increased product shelf-life and globalisation have led to longer and vastly more complicated food chains; potentially presenting greater opportunity for contamination and microbial growth to occur. Modern food safety management systems risk assess hazards (based on current scientific data) and strive to control these, often extensive and convoluted, food production/supply chains through appropriate good hygiene practices (GHP) and HACCP. Contamination of food as a result of human error can undermine even the most carefully prepared and executed HACCP system – culminating in foodborne infection, outbreaks, reputable damage, and erosion of consumer trust. This paper provides a review on the effect …
Arsenic In Groundwater In South West Ireland - Occurrence, Controls, And Hydrochemistry, Ellen R. Mcgrory, Emma Holian, Alberto Alvarez-Iglesias, Norma Bargary, Eoin Mcgillicuddy, Henry Tiernan, Liam Morrison
Arsenic In Groundwater In South West Ireland - Occurrence, Controls, And Hydrochemistry, Ellen R. Mcgrory, Emma Holian, Alberto Alvarez-Iglesias, Norma Bargary, Eoin Mcgillicuddy, Henry Tiernan, Liam Morrison
Articles
Globally numerous regions have been identified with elevated arsenic within groundwater which can result in potential adverse health risks. In Ireland, a previous national-scale research assessment of groundwater identified isolated clusters of elevated arsenic and indicated that lithology was a major controlling factor on arsenic in groundwater. Complementary comparisons of national-scale and regional-scale groundwater assessments of arsenic are lacking in Europe when compared to other global regions. The aims of this study were to demonstrate the value of a regional-scale groundwater hydrochemistry dataset with an existing national-scale approach, describe anomalies that can become the focus of attention for public health …
Raman Spectroscopy Detects Biochemical Changes Due To Different Cell Culture Environments In Live Cells In Vitro, Mahmoud Gargotti, Esen Efeoglu, Hugh Byrne, Alan Casey
Raman Spectroscopy Detects Biochemical Changes Due To Different Cell Culture Environments In Live Cells In Vitro, Mahmoud Gargotti, Esen Efeoglu, Hugh Byrne, Alan Casey
Articles
The in vitro cell culture environment can impact on cell biochemistry and cell cycle. The manifestation of such substrate-induced changes in cell cycle in the Raman microspectroscopic profiles of cell cultures is investigated at the level of nucleolus, nucleus and cytoplasm. HeLa immortalised human cervical cells and HaCaT dermal cells were cultured on three different substrates, conventional polystyrene cell culture dishes, CaF2 slides as a commonly used Raman substrate, and glass slides coated with collagen rat tail, as a mimic of the extra-cellular matrix (ECM) environment. A cell cycle study, based on percentage DNA content, as determined using propidium iodide …
Combating Staphylococcus Aureus And Its Methicillin Resistance Gene (Meca) With Cold Plasma, Xinyu Liao, Patrick J. Cullen, Donghong Liu, Aliyu Idris Muhammad, Shiguo Chen, Xingqian Ye, Jun Wang, Tian Ding
Combating Staphylococcus Aureus And Its Methicillin Resistance Gene (Meca) With Cold Plasma, Xinyu Liao, Patrick J. Cullen, Donghong Liu, Aliyu Idris Muhammad, Shiguo Chen, Xingqian Ye, Jun Wang, Tian Ding
Articles
The increase in antibiotic resistance has become a global challenge to public health. In this study, an atmospheric cold plasma (ACP) system was applied for combating methicillin-resistant Staphylococcus aureus (MRSA) and its methicillin resistance gene (mecA) during food wastewater treatment. The plate count and flow cytometry methods were employed to estimate the damage in MRSA induced by plasma treatment. A quantitative real-time PCR (qPCR) method was used to assess the plasma-induced degradation of the mecA genes. The inactivation of MRSA and degradation of extracellular (e-) and intracellular (i-)mecA genes were investigated in phosphate buffered solution as a function of plasma …
Industrial Grade 2d Molybdenum Disulphide (Mos2): An In-Vitro Exploration Of The Impact On Cellular Uptake, Cytotoxicity, And Inflammation, Caroline Moore, Hugh Byrne, Jonathan N. Coleman, Yuri Volkov, Jennifer Mcintyre
Industrial Grade 2d Molybdenum Disulphide (Mos2): An In-Vitro Exploration Of The Impact On Cellular Uptake, Cytotoxicity, And Inflammation, Caroline Moore, Hugh Byrne, Jonathan N. Coleman, Yuri Volkov, Jennifer Mcintyre
Articles
The recent surge in graphene research, since its liquid phase monolayer isolation and characterization in 2004, has led to advancements which are accelerating the exploration of alternative 2D materials such as molybdenum disulphide (MoS2), whose unique physico-chemical properties can be exploited in applications ranging from cutting edge electronic devices to nanomedicine. However, to assess any potential impact on human health and the environment, the need to understand the bio-interaction of MoS2 at a cellular and sub-cellular level is critical. Notably, it is important to assess such potential impacts of materials which are produced by large scale production techniques, rather than …