Food Science Commons^™

Scientific Works Of Art Reveal A Hidden World, Gary Bauchan, Jan Suszkiw

Agricultural Research Magazine

It has been said that a picture is worth a thousand words, and at the Agricultural Research Service’s Electron and Confocal Microscopy Unit (ECMU) in Beltsville, Maryland, this adage couldn’t be more true. Led by unit director Gary Bauchan, the ECMU is tasked with producing highresolution images that provide a window to the extraordinary world of the unseen.

“We have observed viruses, bacteria, fungi, nematodes, insects, mites, and parasites that threaten global food security, and we’ve contributed to the discovery of how pathogens spread by helping elucidate their relationship to the environment, hosts, and vectors,” says Bauchan. “We’ve also described …

Novel Ways To Combat Plant Pathogens, Deborah Fravel, Gail C. Wisler

Agricultural Research Magazine

Plant diseases must be managed to successfully and reliably produce crops to meet humanity’s growing food, fiber, feed, and fuel needs. Plant disease management relies on many different types of tools—from disease-resistant plant varieties and pesticides to cultural and biological strategies. Agricultural Research Service scientists are seeking new methods of managing plant diseases, more efficient means of using older methods, and combinations of these methods. Two of the oldest ways of reducing plant diseases are crop rotation and organic amendments to soil.

In Orono, Maine, for example, ARS scientists are evaluating a holistic approach to suppressing soilborne pathogens of potato, …

Possible Links Between Soil Microbial Communities And Stroke Risk, Ann Perry

Agricultural Research Magazine

“A big part of our Agricultural Research Service culture is cross-talking with outside researchers,” says ARS soil and water scientist Patrick Hunt. “Partnerships are what we do.”

So one day in 2011, Hunt called Medical University of South Carolina professor Daniel Lackland to discuss a paper Lackland had published about stroke risk in the state. South Carolina is part of the U.S. “Stroke Belt,” where residents have a significantly higher incidence of stroke than the rest of the U.S. population. South Carolina counties with the highest rates of stroke—between 89 and 115 cases per 100,000 residents—are found within the Southern …

Fungal Foam Tested Against Avocado Threat, Jan Suszkiw

Agricultural Research Magazine

Avocados aren’t just nutritional powerhouses; they’re also the chief ingredient in such party favorites as guacamole dip.

More than 99 percent of the nation’s $322 million avocado crop is grown in south Florida and southern California (less than 1 percent is produced in Hawaii), which makes recent infestations of groves there by invasive, wood-boring ambrosia beetles so alarming. A host of counter strategies are in the works, including a biobased foam originally developed by Agricultural Research Service scientists for use against Formosan subterranean termites.

In Miami-Dade County, Florida, avocado growers are contending with Xyleborus glabratus, the redbay ambrosia beetle. …

Fire Ant Venom Compounds May Be Useful As A Fungicide, Jan Suszkiw, Jian Chen, Xixuan Jin

Agricultural Research Magazine

Red imported fire ants are named for the firelike burn of their sting. Now, the same venom that packs such a painful wallop may actually do some good for a change.

Studies by scientists at the Agricultural Research Service’s Biological Control of Pests Research Unit in Stoneville, Mississippi, have shown that certain alkaloid compounds in the venom—namely, piperideines and piperidines—can hinder growth of Pythium ultimum, a top crop pathogen worldwide.

Chemical controls, delayed plantings, and crop rotation are among methods now used against P. ultimum, which causes damping-off diseases that decay the seed or seedlings of vegetable, horticultural, …

August 2013- Locations Featured In This Magazine Issue

Agricultural Research Magazine

Albany, California

9 research units ■ 241 employees

San Joaquin Valley Agricultural Sciences Center, Parlier, California

3 research units ■ 125 employees

Northwest Watershed Research Center, Boise, Idaho

1 research unit ■ 18 employees

Tucson, Arizona

2 research units ■ 54 employees

U.S. Sheep Experiment Station, Dubois, Idaho

1 research unit ■ 10 employees

Fort Keogh Livestock and Range Research Laboratory, Miles City, Montana

1 research unit ■ 25 employees

Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska

6 research units ■ 117 employees

Grazinglands Research Laboratory, El Reno, Oklahoma

2 research units ■ 43 employees

National …

Ars Validates Soil Moisture Data Collected Via Satellite, Ann Perry

Agricultural Research Magazine

The European Space Agency’s Soil Moisture and Ocean Salinity (SMOS) mission includes the latest advance in using Earth-orbiting satellites for estimating soil moisture across the globe. The SMOS satellite was launched in 2009 and was designed to estimate soil moisture levels to within 4 percent, which is like measuring a teaspoon of water in a handful of dry soil.

To capture this data, SMOS uses a new sensor technology that is the first passive L-band system—measuring microwave radiation emitted around the frequency of 1.4 gigahertz—in routine operation. But the accuracy of the information collected by this new technology still needs …

New Strategies To Thwart Pecan Scab, Sharon Durham

Agricultural Research Magazine

Pecans are great for eating out of the shell or in a myriad of recipes. But abundant pickings of high-quality nuts are only possible if the tree escapes the devastating disease called “pecan scab.” Caused by the fungus Fusicladium effusum, it is the most destructive disease of pecan in the southeastern United States. When scab is severe, most often when rainfall is above average, nut size is reduced, and total crop loss might occur.

Scientists at the Southeastern Fruit and Tree Nut Research Laboratory in Byron, Georgia, are working to help pecan growers mitigate the effect of pecan scab. …

Screening Fresh Oranges With Uv Study Pinpoints New Value Of Detection Tactic, David M. Obenland, Joseph L. Smilanick, Marcia Wood

Agricultural Research Magazine

Fresh, deliciously sweet navel oranges, on display at your local supermarket, may have been quickly inspected with ultraviolet (UV) light when they were still at the packinghouse. Usually, the purpose of this special sorting and screening is to see if circular spots—which glow a bright, fluorescent yellow and may be about the size of a quarter or larger—show up on the fruit’s peel.

More often than not, these spots, which scientists refer to as “lesions,” are telltale indicators of the presence of microbes that cause decay, namely Penicillium italicum, responsible for blue mold, or P. digitatum, the culprit …

Agricultural Research Magazine August 2013

Agricultural Research Magazine

Table of Contents

4 Prescription for Curing Citrus Greening: Apply Heat and Wait

7 Screening Fresh Oranges With UV: Study Pinpoints New Value of Detection Tactic

8 Breed Matters: Selecting Rams for Rangeland Production

10 Scientific Works of Art Reveal a Hidden World

13 ARS Validates Soil Moisture Data Collected Via Satellite

14 New Strategies To Thwart Pecan Scab

15 Fire Ant Venom Compounds May Be Useful as a Fungicide

16 Possible Links Between Soil Microbial Communities and Stroke Risk

18 Early Weaning: A Good Bet for Beef Producers in Drought-Stricken Areas

20 Fungal Foam Tested Against Avocado Threat

21 …

Effects Of Composition And Separation Techniques On The Structure Of Aggregates In The Milk Fat Globular Membrane, Tristan Dilbeck, Jose Ibarra, Rafael Jimenez-Flores, Tracey Nguyen

STAR Program Research Presentations

The milk fat globular membrane (MFGM) is a complex structure composed mainly of phospholipids, sphingolipids, and membrane specific proteins. The composition and size of the MFGM differs based on the isolation, purification, and techniques used in analysis of the membrane as well as physiological, chemical, and mechanical factors present during its creation. The purpose of this experiment is to analyze the effects of separation techniques and composition of lipids and proteins on the structure of aggregates in the MFGM. The aggregates found in whey protein isolate (95% pure whey protein), whey buttermilk (whey protein and phospholipids), and sweet buttermilk (whey …

The Effects Of Microfluidization And Homogenization On The Composition And Structure Of Liposomal Aggregates From Whey Buttermilk And Commercial Buttermilk, Tracey Mai T. Nguyen

Master's Theses

Milk derived ingredients from the production of cheese and butter can be used as vehicles for nutrients. Buttermilk is a nutritious product of milk that comes from the churning of cream into butter. One of the advantages of buttermilk is that it is enriched in milk fat globule components, such as phospholipids and forms emulsions with fat when treated with high shear. The objective of this work was to explore the effects of shear on regular buttermilk and whey buttermilk in terms of liposomal aggregate size and chemical composition. The effects of microfluidization at 2000 psi and homogenization at 2000 …

Characterization Of Bacillus Bacterial Strains; Optimum Growth Temperature, Proteolytic And Lipolytic Activity, Jamie Anne Boring

STAR Program Research Presentations

Characterization of Bacillus bacterial strains is an important step in identifying and removing biofilms contaminating processing equipment. These biofilms lead to large amounts of spores in the final product. Strains that produce spoilage enzymes cause off-flavors, structural defects and reduced shelf life of milk products. Using four treatments, we determined the best growth protocol for each strain to use in future experiments. Treatment 1: 80 ^o C heat shock and 32 ^o C 24hr incubation, Treatment 2: 80^o C/ 55 ^o C, Treatment 3: 100 ^o C/ 32 ^o C, Treatment 4: 100 ^o C/ 55 ^o C. Our …

Effects Of Xanthan/Locust Bean Gum Mixtures On The Physicochemical Properties And Oxidative Stability Of Whey Protein Stabilized Oil-In-Water Emulsions, Goutham Puli

Masters Theses & Specialist Projects

Scientific evidence shows that dietary intake of the omega-3 polyunsaturated fatty acids is beneficial to human health. Fish oil is a rich source of omega-3 fatty acids. However, fish oil with high levels of omega-3 PUFA is very susceptible to oxidative deterioration during storage. The objective of this study was to investigate the effect of xanthan gum (XG)-locust bean gum (LBG) mixtures on the physicochemical properties of whey protein isolate (WPI) stabilized oil-in-water (O/W) emulsions containing 20% v/v menhaden oil. The O/W emulsions containing XG/LBG mixtures were compared to emulsions with either XG or LBG alone. The emulsions were prepared …

Development Of Tools To Assess The Effects Of Lunasin On Normal Development And Tumor Progression In Drosophila Melanogaster, Gillian E. Jones

Masters Theses & Specialist Projects

Soy contains many bioactive molecules known to elicit anti-cancer effects. One such peptide, Lunasin, has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. In this study we attempt to understand the developmental effects of Lunasin overexpression in vivo and create reagents that will help us understand Lunasin’s anti tumorigenic effects in an intact organism. cDNA encoding lunasin and EGFP-lunasin were cloned into pUAST and microinjected into Drosophila embryos. Tissue-specific overexpression of EGFP-Lun in the resulting transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. …

Development Of Hplc Methods For The Determination Of Water-Soluble Vitamins In Pharmaceuticals And Fortified Food Products, Hung Khiem Trang

All Theses

Though many HPLC methods have been developed and reported in literature for vitamin analysis for the past two decades, applying certain methods directly from literature more than often fails to reproduce the results reported due to many variables of liquid chromatography. This issue was targeted in this project through the examination of chromatographic behaviors of water-soluble vitamins in order to help the analysts better modify methods from literature or even develop new methods from scratch to fit their analytical need with the resources available (e.g., columns, detectors, etc.) in their lab.
The first part of the project investigated the chromatographic …

An Evaluation Of The Nontarget Effects Of Transgenic Bacillus Thuringiensis Maize On Arbuscular Mycorrhizal Fungi In The Soil Ecosystem, Tanya Elizabeth Amy Cheeke

Dissertations and Theses

My dissertation research examined the effect of the cultivation of insect-resistant Bacillus thuringiensis (Bt) maize on the soil environment with a goal of understanding how to obtain a balance between technological advancement and maintenance of a healthy soil ecosystem. Although Bt plants may help to reduce pesticide use, conferring benefits to farm workers and the environment, there are still unresolved questions about how the cultivation of Bt plants affects soil organisms. For this dissertation project, I used 14 different genotypes of Bt maize and non-Bt maize (Zea mays) to investigate the effects of transgenic Bt plants on the colonization ability, …

Influences Of Music Genre And Components On Food Perception And Acceptance, Alexandra Jean Fiegel

Graduate Theses and Dissertations

Managers of consumer goods companies (i.e., restaurants, grocery stores, and bars) have the potential to effectively utilize environmental factors to stimulate desired consumer behaviors. Background music has been identified as one of the most readily manipulated and influential elements to which a shopper or consumer may be exposed to in a service setting. Nevertheless, little is known about the effect of background sound on food perception and acceptance. This research sought whether background music genre and musical components can alter food perception and acceptance, but also to determine how the effect can vary as a function of food type (i.e., …

Kinetic Studies For The Preparation Of Probiotic Cabbage Juice: Impact On Phytochemicals And Bioactivity, Amit Jaiswal, Nissreen Abu-Ghannam

Articles

A kinetic study for the production of probiotic cabbage juice was carried out under controlled pH and dissolved oxygen using several strains of Lactic acid bacteria (LAB). Furthermore, effect of probiotic fermentation on polyphenolic content and antioxidant capacity was investigated. Results showed significant growth in Lactobacillus brevis, Lactobacillus plantarum and Lactobacillus rhamnosus corresponding to 9.19, 9.47 and 10.6 log CFU/ml after 24 h of fermentation, which is satisfying criteria for a food product to be characterized as probiotic. Lactic acid (LA) was the major end product of the fermented cabbage juice attaining the concentrations of 6.97, 9.69 and 12.2 g/l …

Development Of Rapid Detection Methods And Novel Control Measures For Salmonella In Poultry, Si Hong Park

Graduate Theses and Dissertations

This dissertation consists of three research parts: 1) development of rapid detection methods for foodborne pathogens; 2) immune response of chicken cells against Salmonella and bacteriophage P22; 3) evaluation of novel control measures for poultry productions. In order to develop rapid and accurate detection methods for foodborne pathogens, two types of PCR assays were utilized. Three foodborne pathogens included Campylobacter, Escherichia coli and Salmonella in watershed were qualitatively and quantitatively detected by multiplex PCR and qPCR (chapter 2). Since Salmonella species are commonly present in poultry and poultry products as well as most popular foodborne pathogen in …

Antibacterial Effects Of Proteases On Different Strains Of Escherichia Coli And Listeria Monocytogenes, Hanan Eshamah

All Dissertations

Escherichia coli O157:H7 and Listeria monocytogenes are pathogens that have received special attention by federal agencies, food safety researchers and food industries due to their economic and human health impact. To reduce the presence of these pathogens, alternative interventions have been studied. However, increasing consumer's demand for natural ingredients has made the investigations of effectiveness of natural antimicrobials necessary. In this study, in vitro antimicrobial activity of bromelain and papain against E. coli JM109 and L. monocytogenes was investigated. Furthermore, actinidin and papain were evaluated to reduce populations of L. monocytogenes strain and three mixed strains of E. coli O157:H7 …

Mutational Analysis Of Sse1 (Hsp110) Suggests An Integral Role For This Chaperone In Yeast Prion Propagation In Vivo, Ciara Moran, Gemma Kinsella, Zai-Rong Zhang, Sarah Perrett, Gary Jones

Articles

ABSTRACT The yeast Hsp110 chaperone Sse1 is a conserved protein that is a noncanonical member of the Hsp70 protein superfamily. Sse1 influences the cellular response to heat stress and has also been implicated in playing a role in the propagation of prions in yeast. Sse1 can seemingly exert its effects in vivo through direct or indirect actions by influencing the nucleotide exchange activity of canonical cytosolic Hsp70s. Using a genetic screen based on the inability to propagate the yeast [PSI+] prion, we have identified 13 new Sse1 mutants that are predicted to alter chaperone function through a variety of different …

Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pecan Residues In Processed Foods, Denise Tran

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Pecan nuts are becoming increasingly popular due to their link to health benefits. However, the presence of undeclared pecan residues in food products can pose serious health risks for pecan-allergic consumers. Currently, analytical methods for the detection of pecan allergens are limited and have not been validated for use by the food industry to assess the effectiveness of allergen control programs. Therefore, the aim of this study was to develop a sandwich-type ELISA to detect and quantify allergenic pecan residues in processed foods. Several varieties of pecan were mixed, roasted, and ground to immunize a goat, sheep, and rabbits. The …

Excretion Of Phenolic Compounds After Consumption Of Fresh Versus Aged Chokeberry Juice In Rats, Sarah Elizabeth Graves

Graduate Theses and Dissertations

Flavonoids, such as anthocyanins, may be responsible for the health benefits of chokeberries, as they are present in large quantities in chokeberries and are potent antioxidant and anti-inflammatory agents. Despite these health properties, previous investigations on the bioavailability of flavonoids illustrate that little of the ingested amount reaches the bloodstream. This study investigated two factors that may help to explain these inconsistencies. First, storage of berry fruits and berry fruit products leads to extensive polymerization of their anthocyanins with procyanidins. Additionally, after consumption flavonoids may undergo metabolism into phenolic acids and subsequent conjugation to an extent that may explain their …

The View From The Table: An Analysis Of Participant Reactions To Community-Based Dialogues On Food And Justice, Jennifer Turner

Dissertations and Theses

While Portland, Oregon's sustainable food movement wins accolades for explicitly situating itself in opposition to the industrialized global food system, it often fails to address systems of oppression that are reproduced within the alternative agri-food movement itself. This demonstrated aversion towards the messy, complex, contingent nature of the social world reflects larger processes of "de-politicization" of the overall sustainability agenda, which leads to the favoring of technological and/or spatial solutions that may undermine the social equity and justice dimensions of the "triple bottom line." This thesis focuses on an action research project involving a series of community dialogues that provided …

Fearless: Heather Thomas, Heather L. Thomas

SURGE

This week we would like to recognize recent graduate and AmeriCorps VISTA volunteer Heather Thomas ’13, who is returning to Gettysburg as the fearless new coordinator for the Adams County Food Policy Council. [excerpt]

Milking The System: Do Poor People Deserve Fresh Food?, Melanie M. Meisenheimer

SURGE

Poor Americans are all lazy, selfish people who must first prove their worth as human beings if they want to be able to feed their children.

It sounds harsh, stereotypical, and judgmental when you put it like that, and few people would feel comfortable saying that exact phrase. However, it’s a perception of poverty in America that I’ve found still has a strong grip on our way of thinking. [excerpt]

Storage Technique Preserves Citrus, Kills Pathogens, Gayle Volk, Jan Suszkiw

Agricultural Research Magazine

Americans consume about 84 pounds of fresh and processed citrus per person each year, with oranges topping the list at 61 pounds annually. The availability of these favored fruits in grocery stores, fresh markets, and other consumer outlets is a testament to growers’ success in managing the ever-present threat of pests and diseases to the nation’s $3.4 billion citrus crop (2011-12), most of which is produced in Florida and California.

A major threat is citrus greening disease, also known as “Huanglongbing.” First detected in Florida in August 2005, citrus greening today is the target of a multifaceted effort by federal, …

Detecting Deadly Colonies Of E. Coli, Rosalie Marion Bliss

Agricultural Research Magazine

Shiga-toxin-producing Escherichia coli (STEC) serogroup O157:H7 has long been associated with severe forms of foodborne illnesses. But these bacterial bad guys have many cousins, and six other STEC groups have also been linked to serious illnesses and outbreaks of disease.

In 2011, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced a regulation that, as of June 2012, certain beef products that test positive for any of these six groups, nicknamed the “Big Six,” would be banned from being sold for public consumption. As USDA’s regulatory arm, FSIS employs inspectors who collect meat samples on the front …

Current Challenges Can Help Fuel Future Opportunities, Jeffrey J. Steiner

Agricultural Research Magazine

Native juniper trees and invasive plants pose an expanding threat to the survival of the sagebrush ecosystem in arid western rangelands. As the trees mature, they compete with other native plants that are valuable food sources for wildlife and cattle. After these plants die back, the bare patches of soil that remain are vulnerable to erosion and encroachment from other species like cheatgrass, an invasive nonnative annual that fuels wildfires.

Wildfires threaten livestock ranches, are suppressed in large part by efforts funded with taxpayer dollars, and increase the probability that invasive plants will spread and thrive in the postfire landscape. …